Device and method for preparing gastric cancer organoid

Abstract

The invention discloses a device and method for preparing gastric cancer organs, and belongs to the technical field of cell culture. Comprising a carrying seat, a culture mechanism is arranged in the carrying seat, a culture cavity block is arranged at the top end of the carrying seat, a putting mechanism is arranged on one side of the culture cavity block, and through arrangement of the culture mechanism, the putting mechanism and a treatment mechanism, a fixed carrier and an offset assembly can rapidly perform timely batch treatment on gastric cancer tissue samples; then the sample is sent to a lower culture point by the point injection part, so that activity loss of the sample due to long-time contact with the outside is reduced, matching and multi-batch culture are carried out on the gastric cancer cells in a short time, the operation time is shortened, meanwhile, the formation condition and the growth state of the organoid can be observed in the cavity, and when the cell state has a problem, the operation is convenient. And through the cooperative use of the other group of offset component and the point injection component, the culture medium and the digestion liquid are accurately fed into the cells in time under the conditions that the personnel contact is reduced and the existing culture environment is not damaged.

Description

Technical Field

[0001] The present invention relates to the technical field of cell culture, and in particular to a device and method for preparing gastric cancer organoids. Background Art

[0002] Gastric cancer organoid cell culture refers to culturing tumor cells or stem cells from gastric cancer patients under specific culture conditions to form a growth environment similar to the gastric cancer tissue in the patient's body. During the culture process, gastric cancer cells will gradually form three-dimensional cell spheres or cell clusters. In general, the cultured gastric cancer cells can be used as substitutes for drug testing and disease research, providing clinicians with a more intuitive and reliable basis for selecting anti-gastric cancer drugs;

[0003] However, in actual operation, various problems may arise. For example, if the matrix gel is not handled properly, it will solidify and cause culture failure. When the organoid culture needs to add culture medium, the cells will be exposed to the outside air when the culture liquid is taken out. However, the cells will die due to contamination by microorganisms such as bacteria and fungi. Frequent switching of the culture device will also damage the culture ecosystem inside the device. At the same time, during the culture, the personnel have limited energy because they need to culture multiple experimental subjects. As the culture progresses, the subsequent culture efficiency will become slower and slower, causing the gastric cancer cells to lose some vitality, resulting in an increased probability of subsequent failure. Summary of the invention

[0004] The purpose of the present invention is to provide a device and method for preparing gastric cancer organoids, which solves the problems in the background technology.

[0005] To achieve the above-mentioned purpose, the present invention provides the following technical solution: A device for preparing gastric cancer organoids, comprising a carrier, a culture mechanism is arranged inside the carrier, a culture cavity block is arranged at the top of the carrier, a delivery mechanism is arranged on one side of the culture cavity block, a carrier plate is fixedly installed on the top of the inner wall of the culture cavity block, a processing mechanism is rotatably connected inside the carrier plate, and a closed door is hinged on the surface of the carrier via a hinge.

[0006] Furthermore, the culture mechanism includes a rotating rod arranged inside the carrier, the top of the rotating rod is fixedly connected to a simulated stomach, the surface of the simulated stomach is movably provided with a plurality of culture cavities, the bottom end of the rotating rod is fixedly connected to a toggle ring body, and the surface of the toggle ring body is provided with anti-slip grooves.

[0007] Furthermore, the delivery mechanism includes a fixed carrier arranged on one side of the carrier, two groups of circular liquid storage tanks are arranged inside the fixed carrier, liquid holes corresponding to the circular liquid storage tanks are arranged on the surface of the fixed carrier, a flat-mouth bottle plug is arranged inside the liquid holes, a sealing block is arranged at the inner upper end of the circular liquid storage tank, and a liquid guiding hose is arranged near the sealing block inside the two groups of circular liquid storage tanks.

[0008] Furthermore, the processing mechanism includes a movable ring body rotatably connected to the inside of the carrier plate, and two groups of semicircular carriers are symmetrically arranged inside the movable ring body. The surfaces of the two groups of semicircular carriers are threadedly connected to straight plate carriers, and the surfaces of the straight plate carriers begin to have several groups of rectangular grooves, one of which has an offset component arranged inside the rectangular groove. A cylinder is arranged on the upper end of the straight plate carrier, and a gear pump is fixedly connected to the side of the upper end of the straight plate carrier away from the cylinder, and the interior of the gear pump is connected to the end of the liquid guide hose.

[0009] Furthermore, the surface of the offset assembly is slidably connected with four groups of sliding blocks, the surface of each sliding block is rotatably connected with a movable rod body, the surface of the movable rod body is slidably connected with a guide block body, the upper end of the guide block body is fixedly connected with an L-shaped block, the lower surface of the L-shaped block is fixedly connected to the output end of the cylinder, and the side of the sliding block surface away from the movable rod body is connected to the injection component through a thread.

[0010] Furthermore, a liquid guide tube body is clamped inside the injection component, a lower end of the liquid guide tube body is fixedly connected to a lower liquid needle body and the interiors are interconnected, a correction block is also provided on the surface of the liquid guide tube body, an upper end of the liquid guide tube body is fixedly connected to a liquid flow hose, each end of the liquid flow hose is fixedly connected to a loading arc block, and the interior of the loading arc block is connected to the interior of the gear pump.

[0011] Furthermore, a plurality of groups of flow holes are provided on the surface of the straight plate carrier at positions staggered from the offset assembly.

[0012] Furthermore, a threaded hole is provided at the top of the movable ring body, and a rotating handle is threadedly connected through the threaded hole.

[0013] Furthermore, heating plates are arranged at the edges of both sides of the inner wall of the culture chamber block, and the heating plates on the inner wall of the culture chamber block close to the carrier plate are not in contact with the sealed door.

[0014] A method for preparing a gastric cancer organoid device, the method of use is as follows:

[0015] First, the researchers obtained gastric cancer tissue samples from the patient. Then, they cleaned the samples in time, removed excess tissue, and retained the lesion. Then, they crushed the useful parts into 1-3 mm 3 At this time, turn on the heating plate to heat the inside of the culture chamber block to 37 degrees. At the same time, preheat the flow hose, the liquid guide tube body and the liquid guide hose. Then, add an appropriate amount of tissue digestion liquid to the shredded tissue and observe its digestion status. When cell clusters below 70um are generated, filter the digested liquid with a cell screen, collect the filtrate and centrifuge it, and remove the supernatant.

[0016] Subsequently, a proper amount of matrix gel is placed into one of the circular liquid reservoirs of the delivery mechanism. When the matrix gel is injected, the processed cell sediment is injected into the circular liquid reservoir to form a cell suspension. At this time, the gear pump connected to one set of liquid guide hoses is started. As the gear pump works, the cell suspension inside the delivery mechanism is guided to the inside of the loading arc block. Since the gear pump is connected to the liquid guide hose and the loading arc block, the matrix gel will not coagulate when it is guided to the specified position. At this time, after the inoculated cells are sent to the inside of the loading arc block, they are then separated into several groups and circulated to the inside of the liquid flow hose. , and then enters the interior of the catheter body. It should be noted that the internal parameters of the culture cavity block should be controlled at 37°C and 5% CO2 at this time. Then, the personnel will close the sealed door to create a constant temperature inside the culture cavity block to avoid temperature loss. Then, turn the dial ring under the rotating rod to make the surface culture cavity of the simulated stomach perpendicular to the catheter body. At this time, the liquid enters the interior of the lower liquid needle through the catheter body, and then falls into the interior of the culture cavity. When the organoid mixture has been dripped into the interior of the culture cavity, the culture medium is injected into another circular liquid storage tank. At the same time, the working gear pump is turned off. Open the corresponding cylinder. When the cylinder works, it will drive the L-shaped block to operate. At this time, the L-shaped block will drive the guide block to move up and down. When the guide block works upward or downward, it will drive each movable rod on it to work, so that the movable rod drives each corresponding sliding block to slide, and the liquid guide tube body expands outward or slides inward. At this time, the liquid guide tube body will move to the corresponding culture cavity below where no liquid is being discharged. Then the gear pump works to send the mixed liquid into the culture cavity, and then repeat this operation until all the culture cavities in the same row are filled. After the liquid delivery is completed, the gear pump and the cylinder stop working, and the handle at the top of the movable circle is rotated to rotate it to the other side, and then the liquid is delivered to the lower culture chamber on the other side. At this time, another set of relative gear pumps and cylinders start working. When culture medium needs to be added, the supplementary culture medium is delivered to the culture chamber where the liquid delivery has been completed. It should be noted that the amount of culture medium delivered must be strictly calculated so that it can be delivered to multiple culture chambers simultaneously, or other auxiliary reagents can be added, such as growth factors, antibiotics, inhibitors, etc., to optimize the growth conditions of organoids, or perform experimental interventions;

[0017] If it is necessary to simulate the complex structure of the extracellular matrix in the body, remove the culture cavity and perform the above-mentioned operation steps on the simulated stomach to provide a three-dimensional growth environment for gastric cancer organoids.

[0018] Compared with the prior art, the present invention has the following beneficial effects:

[0019] The present invention provides an apparatus and method for preparing gastric cancer organoids. Through the arrangement of a culture mechanism, a delivery mechanism and a processing mechanism, a fixed carrier and an offset component can quickly and timely process gastric cancer tissue samples in batches, and then the samples are delivered to the culture points below by an injection component, thereby reducing the loss of activity of the samples due to long-term contact with the outside world, and gastric cancer cells are proportioned and cultured in multiple batches in a short time, thereby reducing the operation time. At the same time, the formation and growth status of the organoids can be observed in the cavity. When problems occur in the cell status, another set of offset components and the injection component are used in coordination, and the culture medium and the passage digestion fluid can be accurately dosed to the cells in a timely manner while reducing personnel contact and without destroying the existing culture environment. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 It is an overall schematic diagram of the device of the present invention;

[0021] Figure 2 It is a schematic diagram of the overall structure of the device of the present invention from a second viewing angle;

[0022] Figure 3 It is a schematic diagram of the cross-sectional structure of the entire device of the present invention;

[0023] Figure 4 It is a schematic diagram of a partial cross-section of the entire device of the present invention and a partial second viewing angle structure;

[0024] Figure 5 It is a schematic diagram of the processing mechanism structure of the present invention;

[0025] Figure 6 It is a schematic diagram of the flipping structure of the processing mechanism of the present invention;

[0026] Figure 7 It is a schematic diagram of a half-section structure of the processing mechanism of the present invention;

[0027] Figure 8 It is a further cross-sectional structural schematic diagram of the processing mechanism of the present invention;

[0028] Fig. 9 It is a schematic diagram of the disassembled structure of the processing mechanism part of the present invention;

[0029] Fig.10 It is a schematic diagram of the offset assembly of the present invention;

[0030] Fig.11 It is a schematic diagram of the structure of the gear pump, the liquid guiding tube body and the liquid lowering needle body of the present invention;

[0031] Fig.12 It is a schematic diagram of the disassembled structure of the liquid guiding tube body and the liquid injection needle body of the present invention.

[0032] In the figure: 1. carrier; 2. culture mechanism; 21. rotating rod; 22. simulated stomach; 23. toggle ring; 3. culture cavity block; 4. delivery mechanism; 41. fixed carrier; 42. flat bottle stopper; 43. blocking block; 44. liquid guiding hose; 5. carrier plate; 6. processing mechanism; 61. movable ring; 62. semicircular carrier; 63. straight carrier; 64. rectangular slide; 65. offset assembly; 651. sliding block; 652. movable rod; 653. guide block; 654. L-shaped block; 655. injection component; 6551. liquid guiding tube; 6552. liquid lowering needle; 6553. correction block; 6554. liquid flow hose; 6555. loading arc block; 66. cylinder; 67. gear pump; 7. closed door. DETAILED DESCRIPTION

[0033] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0034] In order to further understand the content of the present invention, the present invention is described in detail in conjunction with the accompanying drawings.

[0035] Combination Figure 1-Figure 10 , comprising a carrier 1, a culture mechanism 2 is arranged inside the carrier 1, a culture cavity block 3 is arranged at the top of the carrier 1, a delivery mechanism 4 is arranged on one side of the culture cavity block 3, a carrier plate 5 is fixedly installed on the top of the inner wall of the culture cavity block 3, a processing mechanism 6 is rotatably connected inside the carrier plate 5, and a closed door 7 is hinged on the surface of the carrier 1 through a hinge.

[0036] The sealed door 7 and the culture chamber block 3 are both made of glass, so that personnel can observe the organoid culture status inside the device in time and take photos for preservation when operating the device. During the specific implementation of the culture, it is necessary to take photos of the culture site regularly for preservation.

[0037] Combination Figure 2-Figure 12 The culture mechanism 2 includes a rotating rod 21 arranged inside the carrier 1, the top of the rotating rod 21 is fixedly connected to a simulated stomach 22, the surface of the simulated stomach 22 is movably provided with a plurality of culture cavities, the bottom end of the rotating rod 21 is fixedly connected to a toggle ring 23, the surface of the toggle ring 23 is provided with anti-slip grooves.

[0038] The delivery mechanism 4 includes a fixed carrier 41 arranged on one side of the carrier 1, and two groups of circular liquid storage tanks are arranged inside the fixed carrier 41. Liquid holes corresponding to the circular liquid storage tanks are arranged on the surface of the fixed carrier 41. A flat-mouth bottle plug 42 is arranged inside the liquid hole. A sealing block 43 is arranged at the upper end of the inner part of the circular liquid storage tank. A liquid guiding hose 44 is arranged near the sealing block 43 inside the two groups of circular liquid storage tanks.

[0039] The processing mechanism 6 includes a movable ring body 61 rotatably connected to the inside of the carrier plate 5, and two groups of semicircular carriers 62 are symmetrically arranged inside the movable ring body 61. The surfaces of the two groups of semicircular carriers 62 are connected to straight plate carriers 63 through threads, and the surface of the straight plate carrier 63 begins to have several groups of rectangular slide grooves 64, one of the rectangular slide grooves 64, and an offset component 65 is arranged inside the rectangular slide groove 64. The upper end of the straight plate carrier 63 is provided with a cylinder 66, and the upper end of the straight plate carrier 63 is fixedly connected to a gear pump 67 away from the cylinder 66. The interior of the gear pump 67 is connected to the end of the liquid guide hose 44.

[0040] The surface of the offset assembly 65 is slidably connected with four groups of sliding blocks 651, the surface of each sliding block 651 is rotatably connected with a movable rod 652, the surface of the movable rod 652 is slidably connected with a guide block 653, the upper end of the guide block 653 is fixedly connected with an L-shaped block 654, the lower surface of the L-shaped block 654 is fixedly connected to the output end of the cylinder 66, and the side of the surface of the sliding block 651 away from the movable rod 652 is connected to the injection component 655 through a thread.

[0041] The four groups of sliding blocks 651 slide on the surface of the offset assembly 65 , and the guide block 653 is pulled by the L-shaped block 654 through the output end of the cylinder 66 so that the four groups of sliding blocks 651 are directional expanded or contracted.

[0042] The inside of the injection component 655 is clamped with a liquid guide tube body 6551, the lower end of the liquid guide tube body 6551 is fixedly connected to a lower liquid needle body 6552 and the interiors are interconnected, a correction block 6553 is also provided on the surface of the liquid guide tube body 6551, the upper end of the liquid guide tube body 6551 is fixedly connected to a liquid flow hose 6554, each end of the liquid flow hose 6554 is fixedly connected to a loading arc block 6555, and the interior of the loading arc block 6555 is communicated with the interior of the gear pump 67.

[0043] The cell suspension inside the delivery mechanism 4 will be guided to the inside of the loading arc block 6555 due to the operation of the gear pump 67. Since the gear pump 67 is connected to the liquid guide hose 44 and the loading arc block 6555, the correction block 6553 is used to make a certain degree of interval correction between the liquid guide tube body 6551 and the sliding block body 651 when the liquid guide tube body 6551 is in motion, so as to avoid unnecessary skewing, thereby causing the lower liquid needle body 6552 to be unable to be vertical and causing liquid delivery problems.

[0044] The surface of the straight plate carrier 63 is provided with a plurality of groups of flow holes at positions staggered from the offset assembly 65 .

[0045] Several groups of flow holes are opened on the surface of the straight plate carrier 63 so that the injection component 655 on the device can be heated in time to reach the set temperature, and at the same time, the weight of the material is reduced so that the injection component 655 can operate more stably.

[0046] A threaded hole is formed at the top of the movable ring body 61, and a rotating handle is threadedly connected through the threaded hole.

[0047] The rotating handle of the movable ring body 61 is for better operating the movable ring body 61, because manual operation by personnel is also an important part in cell culture. By controlling the key parts of personnel, the survival rate of cell culture can be made higher.

[0048] Heating plates are arranged at the edges of both sides of the inner wall of the culture chamber block 3 , and the heating plates on the inner wall of the culture chamber block 3 close to the carrier plate 5 are not in contact with the sealed door 7 .

[0049] The upper and lower groups of heating plates preheat the fluid hose 6554, the fluid guide tube body 6551 and the fluid guide tube 44 in order to quickly reach an appropriate culture temperature inside the device so that cells can be better cultured inside the device.

[0050] Specific operation steps:

[0051] The heating plate is turned on to heat the inside of the culture chamber block 3 to 37 degrees. At the same time, the liquid flow hose 6554, the liquid guide tube body 6551 and the liquid guide hose 44 are preheated, and a proper amount of matrix glue is put into one of the circular liquid storage tanks of the delivery mechanism 4. After the injection of the matrix glue is completed, the treated cell sediment is injected into the circular liquid storage tank to form a cell suspension. At this time, the gear pump 67 connected to one group of liquid guide hoses 44 is started. As the gear pump 67 works, the cell suspension in the delivery mechanism 4 is guided to the inside of the loading arc block 6555. Since the gear pump 67 is connected to the liquid guide hose 44 and the loading arc block 6555, the matrix glue will not coagulate when it is guided to the specified position. At this time, after the inoculated cells are sent to the interior of the loading arc block 6555, they are then separated into several groups and circulated to the interior of the fluid hose 6554, and then enter the interior of the liquid guide tube 6551. It should be noted that the internal parameters of the culture cavity block 3 should be controlled at 37°C and 5% CO2 at this time. Then, the personnel close the sealed door 7 to generate a constant temperature inside the culture cavity block 3 to avoid temperature loss. Then, the dial ring 23 under the rotating rod 21 is rotated to make the surface culture cavity of the simulated stomach 22 perpendicular to the liquid guide tube 6551. At this time, the liquid enters the interior of the lower liquid needle 6552 through the liquid guide tube 6551, and then falls into the interior of the culture cavity. When the organoid mixture has been dripped into the interior of the culture cavity, at this time The culture medium is injected into another circular liquid storage tank. At the same time, the working gear pump 67 is closed and the corresponding cylinder 66 is turned on. When the cylinder 66 is working, it will drive the L-shaped block 654 to operate. At this time, the L-shaped block 654 will drive the guide block 653 to move up and down. When the guide block 653 works upward or downward, it will drive each movable rod 652 thereon to work, so that the movable rod 652 drives each corresponding sliding block 651 to slide, and the liquid guide tube 6551 will expand outward or slide inward. At this time, the liquid guide tube 6551 will move to the corresponding culture cavity below where no liquid is discharged, and then the gear pump 67 will work to mix The liquid is delivered into the culture chamber, and then this operation is repeated until all the culture chambers in the same row are delivered. Then, the gear pump 67 and the cylinder 66 stop working, and the handle at the top of the movable ring body 61 is rotated to rotate it to the other side, and then the liquid is delivered to the lower culture chamber on the other side. At this time, another set of relative gear pumps 67 and cylinders 66 start working, and when culture medium needs to be added, the supplementary culture medium is delivered to the culture chamber that has been delivered. It should be noted that the amount of culture medium delivered must be strictly calculated so that it can be delivered to multiple culture chambers simultaneously, or other auxiliary reagents can be added, such as growth factors, antibiotics, inhibitors, etc., to optimize the growth conditions of organoids, or perform experimental interventions.

[0052] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0053] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A device for preparing gastric cancer organoids, comprising a carrier (1), characterized in that: A culture mechanism (2) is arranged inside the carrier (1), a culture cavity block (3) is arranged at the top of the carrier (1), a delivery mechanism (4) is arranged on one side of the culture cavity block (3), a carrier plate (5) is fixedly mounted on the top of the inner wall of the culture cavity block (3), a processing mechanism (6) is rotatably connected inside the carrier plate (5), and a closed door (7) is hinged on the surface of the carrier (1) via a hinge.

2. The device for preparing gastric cancer organoids according to claim 1, characterized in that: The culture mechanism (2) comprises a rotating rod (21) arranged inside the carrier (1); the top end of the rotating rod (21) is fixedly connected to a simulated stomach (22); a plurality of culture cavities are movably arranged on the surface of the simulated stomach (22); the bottom end of the rotating rod (21) is fixedly connected to a toggle ring (23); and the surface of the toggle ring (23) is provided with anti-slip grooves.

3. The device for preparing gastric cancer organoids according to claim 2, characterized in that: The delivery mechanism (4) comprises a fixed carrier (41) arranged on one side of the carrier (1), two groups of circular liquid storage tanks are arranged inside the fixed carrier (41), liquid holes corresponding to the circular liquid storage tanks are arranged on the surface of the fixed carrier (41), a flat-mouth bottle stopper (42) is arranged inside the liquid hole, a sealing block (43) is arranged at the upper end of the inner part of the circular liquid storage tank, and a liquid guiding hose (44) is arranged at a position close to the sealing block (43) inside the two groups of circular liquid storage tanks.

4. The device for preparing gastric cancer organoids according to claim 3, characterized in that: The processing mechanism (6) comprises a movable ring body (61) rotatably connected to the inside of the carrier plate (5), and two groups of semicircular carriers (62) are symmetrically arranged inside the movable ring body (61). The surfaces of the two groups of semicircular carriers (62) are connected to straight plate carriers (63) through threads, and the surface of the straight plate carrier (63) begins to have a plurality of groups of rectangular slide grooves (64), one of the rectangular slide grooves (64) has an offset component (65) arranged inside the rectangular slide groove (64), and the upper end of the straight plate carrier (63) is provided with a cylinder (66), and the upper end of the straight plate carrier (63) is fixedly connected to a gear pump (67) on the side away from the cylinder (66), and the interior of the gear pump (67) is connected to the end of the liquid guide hose (44).

5. The device for preparing gastric cancer organoids according to claim 4, characterized in that: The surface of the offset assembly (65) is slidably connected to four groups of sliding blocks (651), the surface of each sliding block (651) is rotatably connected to a movable rod (652), the surface of the movable rod (652) is slidably connected to a guide block (653), the upper end of the guide block (653) is fixedly connected to an L-shaped block (654), the lower surface of the L-shaped block (654) is fixedly connected to the output end of the cylinder (66), and the side of the surface of the sliding block (651) away from the movable rod (652) is connected to the injection component (655) through a thread.

6. The device for preparing gastric cancer organoids according to claim 5, characterized in that: The inside of the injection component (655) is clamped with a liquid guide tube body (6551), the lower end of the liquid guide tube body (6551) is fixedly connected to a lower liquid needle body (6552) and the interiors are interconnected. A correction block (6553) is also provided on the surface of the liquid guide tube body (6551), the upper end of the liquid guide tube body (6551) is fixedly connected to a liquid flow hose (6554), and each end of the liquid flow hose (6554) is fixedly connected to a loading arc block (6555), and the interior of the loading arc block (6555) is connected to the interior of the gear pump (67).

7. The device for preparing gastric cancer organoids according to claim 4, characterized in that: The surface of the straight plate carrier (63) is provided with a plurality of groups of flow holes at positions staggered from the offset assembly (65).

8. The device for preparing gastric cancer organoids according to claim 4, characterized in that: A threaded hole is formed at the top of the movable ring body (61), and a rotating handle is threadedly connected through the threaded hole.

9. The device for preparing gastric cancer organoids according to claim 1, characterized in that: Heating plates are arranged at the edges of both sides of the inner wall of the culture chamber block (3), and the heating plates on the inner wall of the culture chamber block (3) close to the carrier plate (5) are not in contact with the sealed door (7).

10. A method for preparing gastric cancer organoids using the device according to any one of claims 1 to 9, characterized in that: The following steps are involved: First, the researchers obtained gastric cancer tissue samples from the patient. Then, they cleaned the samples in time, removed excess tissue, and retained the lesion. Then, they crushed the useful parts into 1-3 mm 3 At this time, the heating plate is turned on to heat the inside of the culture chamber block (3) to 37 degrees. At the same time, the liquid flow hose (6554), the liquid guide tube body (6551) and the liquid guide hose (44) are preheated. Then, an appropriate amount of tissue digestion liquid is added to the shredded tissue, and the digestion condition is observed. When cell clusters below 70um are generated, the digested liquid is filtered through a cell sieve, the filtrate is collected and centrifuged, and the supernatant is removed; Subsequently, a proper amount of matrix gel is placed in one of the circular liquid storage tanks of the delivery mechanism (4). After the injection of the matrix gel is completed, the processed cell precipitate is injected into the circular liquid storage tank to form a cell suspension. At this time, the gear pump (67) connected to one of the groups of liquid guide hoses (44) is started. As the gear pump (67) works, the cell suspension in the delivery mechanism (4) is guided to the inside of the loading arc block (6555). Since the gear pump (67) is connected to the liquid guide hose (44) and the loading arc block (6555), the matrix gel will not coagulate when it is guided to the specified position. At this time, after the inoculated cells are sent to the inside of the loading arc block (6555), they are then separated into several groups and circulated to the liquid flow hose (65 54), and then enters the interior of the liquid guide tube body (6551). It should be noted that the internal parameters of the culture cavity block (3) should be controlled at 37°C and 5% CO2. Then, the personnel closes the sealed door (7) to generate a constant temperature inside the culture cavity block (3) to avoid temperature loss. Then, the dial ring (23) under the rotating rod (21) is rotated to make the surface culture cavity of the simulated stomach (22) perpendicular to the liquid guide tube body (6551). At this time, the liquid enters the interior of the lower liquid needle body (6552) through the liquid guide tube body (6551), and then falls into the interior of the culture cavity. When the organoid mixture has been dripped into the interior of the culture cavity, the culture medium is injected into another circular liquid storage tank. At the same time, the working The gear pump (67) is turned on to open the corresponding cylinder (66). When the cylinder (66) is working, it will drive the L-shaped block (654) to operate. At this time, the L-shaped block (654) will drive the guide block (653) to move up and down. When the guide block (653) is working upward or downward, it will drive each movable rod (652) thereon to work, so that the movable rod (652) drives each corresponding sliding block (651) to slide, and the liquid guide tube (6551) will expand outward or slide inward. At this time, the liquid guide tube (6551) will move to the corresponding culture cavity below where no liquid is being discharged. Then the gear pump (67) will work to send the mixed liquid into the culture cavity. In the cavity, this operation is then repeated until all the culture cavities in the same row are completely supplied with liquid. Then, the gear pump (67) and the cylinder (66) stop working, and the handle at the top of the movable ring body (61) is rotated to rotate it to the other side, and then the liquid is supplied to the lower culture cavity on the other side. At this time, another set of relative gear pumps (67) and cylinders (66) start working. When it is necessary to add culture medium, the supplementary culture medium is delivered to the culture cavity that has been completed with liquid delivery. It should be noted that the amount of culture medium delivered must be strictly calculated so that it can be delivered to multiple culture cavities simultaneously, or other auxiliary reagents, such as growth factors, antibiotics, inhibitors, etc., can be added to optimize the growth conditions of organoids, or to perform experimental interventions; If it is necessary to simulate the complex structure of the extracellular matrix in vivo, the culture cavity is removed and the above-mentioned operation steps are performed on the simulated stomach (22) to provide a three-dimensional growth environment for gastric cancer organoids.

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