Probiotic composition capable of promoting nutrition digestion and absorption and application thereof

Abstract

The invention relates to the technical field of microorganisms, in particular to a probiotic composition capable of promoting nutrition digestion and absorption and application of the probiotic composition. According to the application of the bifidobacterium animalis subsp. Lactis B-01 in preparation of products with the effect of promoting digestion and absorption, experiments prove that the probiotics have the effects of obviously promoting digestion and absorption of intestinal tracts and maintaining health of the intestinal tracts. After a probiotic product composed of animal bifidobacterium subsp. Lactis B-01 is taken, digestion and absorption of intestinal tracts can be effectively promoted. Meanwhile, the probiotics disclosed by the invention have a great application prospect in preparation of products for maintaining intestinal flora balance and health and promoting intestinal digestion and absorption.

Description

Technical Field

[0001] The present invention relates to the technical field of microorganisms, and in particular to a probiotic composition capable of promoting nutrient digestion and absorption and a use thereof. Background Art

[0002] The stomach and intestines are an important place for the human body to digest and absorb nutrients, and are also an important part of the immune protection against harmful substances that enter the intestines. Food that enters the human body needs to be absorbed by the stomach and intestines and converted into nutrients to be transmitted to the human body and the whole body. The digestion and absorption of nutrients is promoted by gastrointestinal peristalsis. If the gastrointestinal peristalsis is slow, it will affect the body's digestion and absorption of nutrients, and then affect development.

[0003] Modern people are under great work pressure, have a tight pace of life, have irregular meals, and eat a diet high in salt, sugar, and fat, which are all harmful to the stomach and intestines. At the same time, when people are angry or depressed, their liver fire will further inhibit the function of the spleen and stomach, making people lose their appetite, thus affecting the digestion and absorption functions. Drugs that promote digestion are mainly used to increase gastrointestinal motility and then play a role in digestion. Generally, there are drugs such as lactase tablets, dry yeast tablets, pepsin tablets, and domperidone tablets. If it is caused by food accumulation due to weak spleen and stomach, it can be treated with stomach-strengthening and digestion-promoting tablets. However, some drugs also have some side effects or only work at the time, treating the symptoms but not the root cause.

[0004] As a type of microecological regulator, probiotics can adjust and maintain the balance of microecology and improve the health of the host through enzyme action, antibacterial, adhesion and colonization, and biological barrier effects. Probiotics have shown good prospects for a variety of health purposes. Undoubtedly, the most popular clinical research field for probiotics is still gastrointestinal diseases, including inflammatory bowel disease (IBD), irritable bowel syndrome (IBS), constipation, diarrhea, functional dyspepsia, and promoting absorption, among which there are some important advances.

[0005] Under normal physiological conditions, intestinal flora plays an important role in the synthesis of vitamins, growth and development, metabolism, and immune defense functions of the human body. It is a necessary factor for maintaining human health and a mirror that reflects the stability of the internal environment of the body. According to existing studies, the main function of intestinal flora is to promote food digestion, further regulate and enhance the absorption of nutrients. Intestinal flora can help the host digest specific foods. For example, dietary fiber cannot be digested and absorbed by the human small intestine, but can be used as food residues by intestinal flora. There are many studies at present, and it is also widely used in the fields of bioengineering, industry and agriculture, food safety, and life and health. There are also many probiotics and compound probiotics that can be used to regulate the intestine. Selecting suitable probiotics and their probiotic compositions to promote intestinal digestion and absorption has gradually become a hot topic in research in this field. Summary of the invention

[0006] Therefore, the technical problem to be solved by the present invention is to provide a probiotic composition capable of promoting nutrient digestion and absorption and its use.

[0007] To this end, the present invention provides the following technical solutions:

[0008] The embodiment disclosed in the present invention relates to the use of animal Bifidobacterium lactis subspecies B-01 in the preparation of a product having the function of promoting nutrient digestion and absorption. The animal Bifidobacterium lactis subspecies B-01 has been deposited in the General Microbiological Center of China National Committee for the Collection of Microbiological Cultures, and its deposit number is CGMCC No.17742.

[0009] In some embodiments, the product includes functional food, health care product or medicine; optionally, the functional food includes beverage, milk powder, protein powder, dairy product, complementary food; the beverage includes solid beverage; the milk powder includes modulated milk powder;

[0010] Optionally, the dosage form of the drug includes a liquid preparation, a semisolid preparation or a solid preparation; optionally, the dosage form of the drug includes a tablet, a powder, a drop, a granule, a capsule or an injection.

[0011] A probiotic composition capable of promoting nutrient digestion and absorption, comprising:

[0012] 6-10 parts by weight of bacterial powder prepared from the animal Bifidobacterium lactis subspecies B-01;

[0013] 80-90 parts by weight of dietary fiber, and / or 5-92 parts by weight of maltodextrin.

[0014] In some embodiments, the number of viable bacteria in the animal Bifidobacterium lactis subsp. B-01 bacterial powder is ≥1.5×10 10 CFU / g;

[0015] Optionally, the number of viable bacteria in the bacterial powder prepared from the animal Bifidobacterium lactis subspecies B-01 is ≥2.0×10 10 CFU / g;

[0016] Optionally, the number of viable bacteria in the animal Bifidobacterium lactis subspecies B-01 bacterial powder is ≥2.5×10 10 CFU / g.

[0017] In some embodiments, the dietary fiber includes at least one of inulin and resistant dextrin;

[0018] Optionally, the dietary fiber includes inulin and resistant dextrin, and the mass ratio of the inulin to the resistant dextrin is 1:1 to 1.2.

[0019] The embodiments disclosed in the present invention relate to the use of the probiotic composition having the function of promoting nutrient digestion and absorption in any of the following:

[0020] (1) Use in the preparation of products that promote digestion and absorption;

[0021] (2) Use in the preparation of products that enhance intestinal peristalsis;

[0022] (3) Use in the preparation of products that increase the activity of protease, lactase, sucrase, aminopeptidase, maltase, cellulase and / or lipase;

[0023] (4) Use in the degradation of proteins, lactose, peptides, maltose, cellulose and / or fat.

[0024] In some embodiments, the product includes food, health products or medicines;

[0025] Optionally, the food includes beverages, milk powder, protein powder, and supplementary food; the beverage includes solid beverage; the milk powder includes modulated milk powder;

[0026] Optionally, the dosage form of the drug includes a liquid preparation, a semisolid preparation or a solid preparation; optionally, the dosage form of the drug includes a tablet, a powder, a drop, a granule, a capsule or an injection.

[0027] The embodiment disclosed in the present invention relates to a method for preparing fermented milk that can promote nutrient digestion and absorption, using the animal Bifidobacterium lactis subspecies B-01 as the strain, and the inoculation amount is ≥1.0×10 7 CFU / g.

[0028] In some embodiments, 88-96 parts by weight of milk are weighed, preheated to 55-60°C, 4-6 parts by weight of sugar are added, the stirring speed is 800-1000rpm, the material is mixed for 10-15min, homogenization is performed at 60-65°C, the primary pressure is 20.0-30.0Mpa, the secondary pressure is 4.5-5.0Mpa, sterilization is performed at 90-95°C for 5-10min, cooling to 36-38°C for inoculation and fermentation, fermentation is performed for 12-14h to pH 4.4-4.6, cooling is performed, and standby. The sugar includes but is not limited to white granulated sugar.

[0029] The embodiments disclosed in the present invention relate to a use of the fermented milk in any of the following:

[0030] (1) Use in the preparation of products that promote digestion and absorption;

[0031] (2) Use in the preparation of products that enhance intestinal peristalsis;

[0032] Optionally, the product includes yogurt, flavored fermented milk, fermented buttermilk, yogurt wine or milk wine.

[0033] The technical solution of the present invention has the following advantages:

[0034] 1. The purpose of the animal Bifidobacterium lactis subspecies B-01 provided by the present invention in preparing a product having the effect of promoting nutrient digestion and absorption, and experiments have shown that the probiotics of the present invention have the effect of significantly promoting intestinal digestion and absorption and maintaining intestinal health. By taking the probiotic products composed of animal Bifidobacterium lactis subspecies B-01, intestinal digestion and absorption can be effectively promoted. At the same time, the probiotics of the present invention have great application prospects in preparing products that maintain the balance and health of intestinal flora and promote intestinal digestion and absorption.

[0035] 2. The present invention provides a probiotic composition capable of promoting nutrient digestion and absorption, comprising: 6-10 parts by weight of bacterial powder prepared from the animal Bifidobacterium lactis subspecies B-01; 80-90 parts by weight of dietary fiber, and / or 5-92 parts by weight of maltodextrin; the present invention has verified that the animal Bifidobacterium lactis subspecies B-01 bacterial powder, inulin, resistant dextrin and maltodextrin have a significant synergistic effect in promoting nutrient digestion and absorption, which is significantly better than a combination containing only animal Bifidobacterium lactis subspecies B-01 bacterial powder and maltodextrin.

[0036] 3. The fermented milk provided by the present invention can promote nutrient digestion and absorption. The fermentation is carried out using the animal Bifidobacterium lactis subspecies B-01 as the strain. Since the obtained fermented milk is rich in the probiotic animal Bifidobacterium lactis subspecies B-01, the fermented milk can have a good effect of promoting digestion and absorption. It can better promote the digestion and absorption of food by rats by enhancing the activity of digestive enzymes, and has a more lasting effect of improving the activity of digestive enzymes. DETAILED DESCRIPTION

[0037] The following examples are provided for a better understanding of the present invention, but are not intended to limit the best mode of implementation, nor to limit the content and protection scope of the present invention. Any product identical or similar to the present invention obtained by anyone under the inspiration of the present invention or by combining the features of the present invention with other prior arts shall fall within the protection scope of the present invention.

[0038] If no specific experimental steps or conditions are specified in the examples, the conventional experimental steps or conditions described in the literature in the field can be used. If no manufacturer is specified for the reagents or instruments used, they are all conventional reagent products that can be obtained commercially.

[0039] The preparation method of the animal Bifidobacterium lactis subspecies B-01 bacterial powder involved in the following Examples 1-4 is as follows: streak the animal Bifidobacterium lactis subspecies B-01 bacterial liquid on MRS solid medium, and culture it anaerobically at 37°C for 24 hours to obtain a single colony; pick a single colony and inoculate it in MRS liquid medium, and culture it anaerobically at 37°C for 24 hours to activate it to obtain an activated liquid; inoculate the activated liquid in an MRS liquid medium (conventionally commercially available) at an inoculum amount of 2% (v / v), and culture it anaerobically at 37°C for 24 hours to obtain a bacterial liquid; centrifuge the bacterial liquid at 4°C and 8000×g for 10 minutes to obtain bacterial cells and a supernatant; wash the bacterial cells three times with PBS buffer and resuspend them in PBS buffer to a bacterial concentration of 1×10 8 CFU / mL, and obtain animal Bifidobacterium lactis subsp. B-01 bacterial suspension. Add freeze-drying protective agent glycerol to the bacterial suspension to a volume percentage concentration of 25% to obtain a mixed solution; vacuum freeze-dry the mixed solution to obtain animal Bifidobacterium lactis subsp. B-01 bacterial powder.

[0040] Inulin, from chicory, content ≥90%, purchased from Beijing Debio Trading Co., Ltd.;

[0041] Resistant dextrin, total dietary fiber content ≥ 90%, purchased from Shandong Xingguang Shouchuang Biotechnology Co., Ltd.;

[0042] Maltodextrin, with a degree of polymerization of 15-20, was purchased from Baolingbao Biotechnology Co., Ltd.

[0043] Examples 1-4

[0044] This embodiment provides a probiotic composition that can promote nutrient digestion and absorption. The specific formula is shown in Table 1.

[0045] Table 1 Probiotic composition formula of Examples 1-3

[0046]

[0047]

[0048] The preparation method of the probiotic composition in the above embodiment is to select raw materials according to the formula and directly mix them evenly.

[0049] Comparative Example 1

[0050] The difference between Comparative Example 1 and Example 2 is that the formula does not contain probiotics, the formula dosage is supplemented with maltodextrin, and the rest is the same as Example 2, see Table 2 for details.

[0051] Table 2 Comparative Example 1 Probiotic Composition Formula

[0052] Ingredients Comparative Example 1 Bifidobacterium animalis subsp. lactis B-01 - Inulin 40 Resistant dextrin 45 Maltodextrin 15

[0053] Example 5 Preparation of fermented milk

[0054] This embodiment provides a method for preparing fermented milk, comprising the following steps:

[0055] Weigh 92 kg of milk, preheat to 58 ° C, add 5 kg of white sugar, use a stirrer to adjust the speed to 900 rpm, mix for 13 min, homogenize at 63 ° C, primary pressure 25.0 MPa, secondary pressure 4.8 MPa, sterilize at 93 ° C, 8 min, cool to 37 ° C and inoculate animal Bifidobacterium lactis subspecies B-01 for fermentation, the inoculum size is 1.0 × 10 7 CFU / g, ferment for 13h to pH 4.4-4.6, cool and set aside.

[0056] Example 6 Preparation of fermented milk

[0057] This embodiment provides a method for preparing fermented milk, comprising the following steps:

[0058] Weigh 88 kg of milk, preheat to 55 ° C, add 6 kg of white sugar, use a stirrer to adjust the speed to 800 rpm, mix for 15 minutes, homogenize at 60 ° C, primary pressure 20.0 MPa, secondary pressure 4.5 MPa, sterilize at 95 ° C for 5 minutes, cool to 36 ° C and inoculate animal Bifidobacterium lactis subspecies B-01 for fermentation, the inoculum size is 1.2 × 10 7 CFU / g, ferment for 14 hours until pH 4.4-4.6, cool and set aside.

[0059] Example 7 Preparation of fermented milk

[0060] This embodiment provides a method for preparing fermented milk, comprising the following steps:

[0061] Weigh 96 kg of milk, preheat to 60 ° C, add 4 kg of white sugar, use a stirrer to adjust the speed to 1000 rpm, mix the material for 10 minutes, homogenize at 65 ° C, primary pressure 30.0 MPa, secondary pressure 5.0 MPa, sterilize at 90 ° C for 10 minutes, cool to 38 ° C and inoculate animal Bifidobacterium lactis subspecies B-01 for fermentation, the inoculum size is 1.5 × 10 7 CFU / g, ferment for 12h to pH 4.4-4.6, cool and set aside.

[0062] Experimental Example 1 Test on the Function of Probiotic Composition in Promoting Nutrition Digestion and Absorption

[0063] 1. Test samples: the probiotic compositions prepared in Examples 1-4 and Comparative Example 1, skim milk powder (New Zealand skim milk powder, purchased from Rongheng Trading Co., Ltd., Hohhot), SD rats (purchased from Sibeifu (Beijing) Biotechnology Co., Ltd.).

[0064] 2. Experimental method: A total of 150 healthy 4-week-old SD rats with no obvious adverse symptoms were selected and divided into 6 groups, with 25 rats in each group. They were divided into an experimental group and a control group. Each group of rats was gavaged with skim milk powder. The experimental group added the probiotic composition to the skim milk powder to obtain a skim milk powder mixture. The probiotic composition mixing ratio was 4% by mass. The control group was only given skim milk powder without adding any composition. During the experiment, SD rats were kept free to eat and eat freely, and adaptive feeding was performed for 7 days (1 week) before the experiment. 4.0 g of skim milk powder or skim milk powder mixture in each group was mixed with 30 mL of water at 42±2℃, and 20 mg (skim milk powder or skim milk powder mixture) / Kg / d was gavaged to rats for 3 times for 4 consecutive weeks. The weight gain of rats was recorded at the beginning of the intervention and 4 weeks of intervention, and the food intake and food utilization rate after 4 weeks of treatment were recorded. Food utilization rate = weight gain of animals during feeding period (g) / total feed consumption during feeding period (g) × 100%. The average value of rats in each group was taken as the final result. Rats were free to eat and drink during the experiment.

[0065] Data statistical methods: In this study, at least three parallel measurements were performed on each group of samples, and the final results were expressed as "mean ± standard deviation". Office Excel was used to summarize the experimental data, and IBM SPSS Statistics 20 was used for data analysis. The significance level was set at 0.05, and the Tukey B method and Waller-Duncan method of one-way analysis of variance were used for pairwise comparison analysis. Origin 2018 and Office Excel were used to draw statistical graphs.

[0066] 3. Experimental results: See Table 3 for specific experimental results.

[0067] Table 3 Effects of different experimental samples on the body weight of SD rats after digestion and absorption

[0068]

[0069] In the comparison between the examples and comparative examples and the control group, * indicates p < 0.05, ** indicates p < 0.01, and *** indicates p < 0.001.

[0070] As can be seen from Table 3, after 4 weeks of intragastric administration, the body weight of rats in each group increased with the extension of intragastric administration time, the body weight and average daily weight gain of rats in Examples 1-4 were significantly higher than those in the control group, the average daily food intake of the groups in Examples 1-4 was significantly lower than that in the control group, but the food utilization rate of the groups in Examples 1-4 was significantly higher than that in the control group. The above shows that the probiotic composition of Examples 1-4 can significantly improve the digestion and absorption function of rats, the weight gain is more obvious, the food utilization rate is improved, and feed can be saved.

[0071] Compared with Comparative Example 1, the rat body weight, average daily weight gain and bioavailability of Examples 1-3 are significantly higher than those of Comparative Example 1, and the average daily food intake is significantly lower than that of Comparative Example 1. The effect of Comparative Example 1 is comparable to that of the control group. The above explanation shows that in Comparative Example 1, animal Bifidobacterium lactis subspecies B-01 powder is not added, and only dietary fiber and maltodextrin cannot play a significant role in promoting digestion and absorption. Compared with Example 4, the average daily weight gain and bioavailability of Example 2 are significantly higher than those of Example 4, and the average daily food intake is significantly lower than that of Example 4. The digestion and absorption promoting effect of Example 2 is significantly better than that of Example 4, and Example 4 only contains animal Bifidobacterium lactis subspecies B-01 powder and maltodextrin. The above explanation shows that there is a significant synergistic effect of promoting digestion and absorption between animal Bifidobacterium lactis subspecies B-01 powder, inulin, resistant dextrin and maltodextrin in Example 2.

[0072] Since the amount of probiotics added in the probiotic composition of Example 1 is lower than that of Examples 2-4, the body weight, average daily weight gain and bioavailability of rats in Example 1 are slightly lower than those in Examples 2-4.

[0073] Experimental Example 2 Evaluation experiment of the effect of small intestinal propulsion movement of composite probiotic composition

[0074] 1. Test samples: probiotic compositions prepared in Examples 1-4 and Comparative Example 1, C57BL / 6J mice (purchased from Sibeifu (Beijing) Biotechnology Co., Ltd.).

[0075] 2. Experimental method: 276 C57BL / 6J mice were selected, which were required to be healthy and without obvious adverse symptoms, and were randomly divided into blank control group, positive control group, low-dose group, medium-dose group and high-dose group, and fed for 2 days as follows:

[0076] Blank control group: free diet;

[0077] Model control group: no water or food restriction;

[0078] Positive control group: fed with Bifikon (Shanghai Xinyi Pharmaceutical Co., Ltd.), 15 mg / kg·d, without water or food restriction;

[0079] Low-dose group: The probiotic composition of the present invention was mixed with sterile distilled water to form a suspension with a concentration of 2 g / L at a dosage of 0.4 g (probiotic composition) / kg·d, and the subjects were gavaged without water or food restriction;

[0080] Medium dose group: The probiotic composition of the present invention was mixed with sterile distilled water to form a suspension with a concentration of 2 g / L at a dosage of 0.8 g (probiotic composition) / kg·d, and the subjects were gavaged without water or food restriction;

[0081] High-dose group: The probiotic composition of the present invention was mixed with sterile distilled water to form a suspension with a concentration of 2 g / L at a dosage of 1.6 g (probiotic composition) / Kg·d, and the suspension was administered by intragastric gavage without water or food restriction.

[0082] The model group and the blank control group were gavaged with a suspension containing 2% ink prepared with sterile distilled water, the positive control group was gavaged with a suspension containing 2% ink prepared with Bifikon, and the low-dose group, the medium-dose group and the high-dose group were gavaged with a suspension containing 2% ink prepared with the probiotic composition of the present invention and sterile distilled water (probiotic composition concentration 2g / L), respectively. The gavage volume of the suspension containing 2% ink in each group was 0.2ml / 20g (body weight). After gavage for 30 minutes, the mice were killed by cervical dislocation, and the stomach and intestine were immediately removed by laparotomy and spread on a glass plate. The moving distance of the charcoal tip in the intestinal tube and the total length of the small intestine (from pylorus to ileum) were measured, and the small intestine advancement percentage was calculated, advancement percentage (%)=distance between the front end of the charcoal ink and the pylorus / total length of the small intestine×100%.

[0083] Data statistical methods: In this study, at least three parallel measurements were performed on each group of samples, and the final results were expressed as "mean ± standard deviation". Office Excel was used to summarize the experimental data, and IBM SPSS Statistics 20 was used for data analysis. The significance level was set at 0.05, and the Tukey B method and Waller-Duncan method of one-way analysis of variance were used for pairwise comparison analysis. Origin 2018 and Office Excel were used to draw statistical graphs.

[0084] 3. Experimental results: See Table 4 for specific experimental results.

[0085] Table 4 Effect of probiotic composition on small intestinal propulsive motility in normal C57BL / 6J mice

[0086]

[0087]

[0088] Compared with the blank control group, the model group, * indicates p < 0.05, ** indicates p < 0.01, *** indicates p < 0.001; compared with the experimental group, positive control group and the model group, # indicates p < 0.05, ##p < 0.01, ### indicates p < 0.001.

[0089] The results in Table 4 show that there is a statistical difference between the model group and the blank control group, indicating that the model was successfully constructed. Compared with the model group, Examples 1-4, the positive control group and Comparative Example 1 can significantly increase the ink propulsion distance and propulsion percentage of the small intestine of mice, indicating that they all have the effect of promoting the propulsion movement of the small intestine.

[0090] Compared with Comparative Example 1, the ink propulsion distance and propulsion percentage of Examples 1-4 are significantly higher than those of Comparative Example 1. The high, medium and low doses of Examples 1-4 and Bifikon can significantly increase the ink propulsion distance and propulsion percentage of the small intestine of mice. With the increase of the dosage, the content of the probiotic composition increases, which increases the ink propulsion distance and propulsion percentage of the small intestine of mice. It shows that the animal Bifidobacterium lactis subspecies B-01 powder added in Examples 1-4 can significantly promote the propulsion movement of the small intestine, and is positively correlated with the added amount.

[0091] Compared with Example 4, the ink propulsion distance and propulsion percentage of Example 2 are significantly higher than those of Example 4. The effect of Example 2 on promoting small intestinal propulsion movement is significantly better than that of Example 4, while Example 4 only contains animal Bifidobacterium lactis subspecies B-01 bacterial powder and maltodextrin. The above shows that the animal Bifidobacterium lactis subspecies B-01 bacterial powder, inulin, resistant dextrin and maltodextrin in Example 2 have a significant synergistic effect on promoting small intestinal propulsion movement and synergistically enhance the function of intestinal peristalsis.

[0092] Experimental Example 3 Effect of probiotic fermented milk on rat digestive enzymes

[0093] 1. Test samples: Probiotics Bifidobacterium lactis subspecies B-01 and Bifidobacterium lactis subspecies Bb-12 (provided by Chr. Hansen (Beijing) Trading Co., Ltd.), which have been proven to have good digestion and absorption and relieve indigestion, were added at 1.0×10 7 CFU / g inoculation amount for yogurt fermentation (preparation method is the same as Example 5), and the number of viable bacteria in the obtained fermented milk is ≥8.6×10 7 CFU / g, SD rats (purchased from Sibeifu (Beijing) Biotechnology Co., Ltd.).

[0094] 2. Experimental method: A total of 75 healthy SD rats with no obvious adverse symptoms were selected and randomly divided into 3 groups, with 25 rats in each group. They were divided into animal Bifidobacterium lactis subspecies B-01 test group (test group 1), animal Bifidobacterium lactis subspecies Bb-12 (test group 2) and control group. During the test, the environmental conditions, basic diet and feeding management of each group were the same. During the test, the SD rats maintained free feeding and free diet, and adaptive feeding was performed for 7 days before the experiment. During the test, the control group was fed with 2mL of normal saline per day on the basis of the basic diet, and the experimental group 1 was fed with 2mL of animal Bifidobacterium lactis subspecies B-01 fermented milk per day on the basis of the basic diet, once a day, and the test period was 28 days; the experimental group 2 was fed with 2mL of animal Bifidobacterium lactis subspecies Bb-12 fermented milk per day on the basis of the basic diet, once a day, and the test period was 28 days. After the end of the 28-day test period, the control group and the experimental group 1 (13 rats) and the experimental group 2 (13 rats) were tested. The remaining rats in the two experimental groups 1 and 2 stopped oral administration of fermented milk for 7 days, and were gavaged with 2 mL of normal saline every day during this period. After gavage with normal saline for 7 days, the test was performed. Before the test, the three groups of SD rats fasted for 24 hours, and then the rats were anesthetized with ether, and the stomach and small intestine were immediately removed by laparotomy, and the stomach and small intestine were ligated. The contents of the stomach and small intestine were quickly removed and stored at -20°C for later use.

[0095] Then the activity of pepsin and digestive enzymes secreted by the small intestine was determined. The contents of the stomach and small intestine were added to normal saline at a weight-to-volume ratio of 1:9 (g / ml), and ultrasonically crushed and homogenized to prepare a 10% sample homogenate. The homogenate was then centrifuged at 3000 r / min for 10 min in a low-temperature low-speed centrifuge, and the supernatant was taken. The activity of pepsin and lactase, sucrase, aminopeptidase, maltase and cellulase in the small intestinal tissue, and trypsin and pancreatic lipase secreted into the small intestine in the pancreatic tissue were detected according to the instructions of the kit (purchased from Nanjing Jiancheng Bioengineering Institute), and the absorbance was determined using an Evolution200 UV spectrophotometer to calculate the activity of proteases and digestive enzymes. A change of 0.01 in A per minute is defined as one unit of enzyme activity: U = [ΔA / (0.01×t)]×D, where: U is enzyme activity; ΔA is the change in absorbance during the reaction time; t is the reaction time (min); and D is the dilution factor, i.e., the total enzyme solution extracted is a multiple of the enzyme solution in the reaction system.

[0096] Data statistical methods: Office Excel was used to summarize the experimental data, and IBM SPSS Statistics 20 was used for data analysis. The significance level was set at 0.05, and the Tukey B method and Waller-Duncan method of one-way analysis of variance were used for pairwise comparison analysis. Origin 2018 and Office Excel were used to draw statistical graphs.

[0097] 3. Experimental results: See Table 5 for specific experimental results.

[0098] Table 5. Effect of adding probiotic animal Bifidobacterium lactis subsp. B-01 fermented milk on digestive enzymes in rats

[0099]

[0100] At 28 days, compared with experimental group 1, experimental group 2 and the control group, * indicates p < 0.05, ** indicates p < 0.01, *** indicates p < 0.001. After 7 days of feeding withdrawal, compared with the control group, # indicates p < 0.05, ##p < 0.01, ### indicates p < 0.001.

[0101] Pepsin is a digestive protease secreted by the chief cells of the gastric mucosa in the stomach, which can break down the protein in food into small peptide fragments. The small intestine is the main site of digestion and absorption, mainly chemical digestion, in which digestive enzymes play an important role. Maltase is an enzyme present in the brush edge of the small intestine, which can catalyze the decomposition of the disaccharide maltose into the monosaccharide glucose, which is more easily absorbed by the epithelial cells of the small intestine; sucrase, also known as invertase, can catalyze and decompose sucrose into glucose and fructose, which is more conducive to the body's absorption; lactase can hydrolyze lactose into glucose and galactose. Lack of lactase will cause diarrhea in patients; aminopeptidase comes from the glands of the small intestine, and its function is to sequentially cut off single amino acids from the amino end of the peptide chain, which is conducive to better absorption; cellulase can promote intestinal peristalsis, increase the moisture in the intestine, thereby softening the stool, and can play a role in moisturizing the intestine and facilitating bowel movements; pancreatic lipase secreted by the pancreas in the small intestine helps digest fat. If the lipase secretion is abnormal, the fatty food ingested by the patient cannot be decomposed and digested; trypsin secreted by the pancreas in the small intestine is a hydrolase that can break down protein into amino acids, which is more easily absorbed by the human intestine and sent to various tissues of the human body. It can play a vital role in the digestion of food in the intestine. Pancreatic amylase and pancreatic lipase are the main digestive enzymes and can be used as indicators to judge the intestinal digestion and absorption function.

[0102] As can be seen from the result of Table 5, feeding SD rats 28d probiotics animal bifidobacterium lactis subspecies B-01 and animal bifidobacterium lactis subspecies Bb-12 fermented milk respectively, the activity of 7 kinds of digestive enzymes in pepsin and small intestine and pancreatic tissue is all improved, but feeding probiotics animal bifidobacterium lactis subspecies B-01 fermented milk improves the activity of 7 kinds of digestive enzymes to be slightly higher than animal bifidobacterium lactis subspecies Bb-12 fermented milk test group, and trypsin and sucrase are more significant (p<0.01).Meanwhile, after stopping gavage fermented milk 7d, feeding probiotics animal bifidobacterium lactis subspecies B-01 fermented milk test group can still keep higher enzyme activity, and animal bifidobacterium lactis subspecies Bb-12 fermented milk test group is then no different from control group, indicating that animal bifidobacterium lactis subspecies B-01 fermented milk of the present invention has a more lasting effect of improving digestive enzyme activity. In summary, it is shown that the animal Bifidobacterium lactis subspecies B-01 probiotic preparation of the present invention has a good effect of promoting digestion and absorption, and can better promote the digestion and absorption of food by rats by enhancing the activity of digestive enzymes.

[0103] A large number of studies have shown that after probiotics enter the intestines, they can not only prevent the proliferation of harmful intestinal bacteria, but also produce a variety of digestive enzymes and organic acids to improve digestion efficiency, thereby achieving the effect of improving feed conversion rate; probiotics can also improve the utilization rate of feed by animals by regulating digestive enzymes, thereby playing a growth-promoting role. This may be because after probiotics enter the intestines, they colonize, grow and reproduce, producing a variety of extracellular enzymes that enter the intestinal "enzyme pool", thereby improving the activity of animal digestive enzymes.

[0104] In summary, the probiotic animal Bifidobacterium lactis subspecies B-01 of the present invention can effectively promote the body's nutrient digestion and absorption. At the same time, the probiotic animal Bifidobacterium lactis subspecies B-01 of the present invention can be used to prepare a variety of probiotic preparations such as solid beverages, modulated milk powder, protein powder or / and medicines, and is used to maintain the health of intestinal flora.

[0105] Obviously, the above embodiments are merely examples for the purpose of clear explanation, and are not intended to limit the implementation methods. For those skilled in the art, other different forms of changes or modifications can be made based on the above description. It is not necessary and impossible to list all the implementation methods here. The obvious changes or modifications derived therefrom are still within the scope of protection of the invention.

Claims

1. Use of animal Bifidobacterium lactis subspecies B-01 in preparing a product having the effect of promoting nutrient digestion and absorption, characterized in that: The animal Bifidobacterium lactis subspecies B-01 has been deposited in the General Microbiological Center of China Microbiological Culture Collection Administration, and its deposit number is CGMCC No.17742.

2. The use according to claim 1, characterized in that The product includes functional food, health care product or medicine; optionally, the functional food includes beverage, milk powder, protein powder, dairy product, complementary food; the beverage includes solid beverage; the milk powder includes modulated milk powder; Optionally, the dosage form of the drug includes a liquid preparation, a semisolid preparation or a solid preparation; optionally, the dosage form of the drug includes a tablet, a powder, a drop, a granule, a capsule or an injection.

3. A probiotic composition capable of promoting nutrient digestion and absorption, characterized in that: include: 6-10 parts by weight of the bacterial powder prepared from the animal Bifidobacterium lactis subspecies B-01 described in claim 1; 80-90 parts by weight of dietary fiber, and / or 5-92 parts by weight of maltodextrin.

4. The probiotic composition capable of promoting nutrient digestion and absorption according to claim 3, characterized in that: The number of viable bacteria in the animal Bifidobacterium lactis subspecies B-01 bacterial powder is ≥1.5×10 10 CFU / g; Optionally, the number of viable bacteria in the bacterial powder prepared from the animal Bifidobacterium lactis subspecies B-01 is ≥2.0×10 10 CFU / g; Optionally, the number of viable bacteria in the animal Bifidobacterium lactis subspecies B-01 bacterial powder is ≥2.5×10 10 CFU / g; Optionally, the dietary fiber includes at least one of inulin and resistant dextrin; Optionally, the dietary fiber includes inulin and resistant dextrin, and the mass ratio of the inulin to the resistant dextrin is 1:1 to 1.

2.

5. Use of the probiotic composition capable of promoting nutrient digestion and absorption according to any one of claims 3 to 4 in any of the following: (1) Use in the preparation of products that promote digestion and absorption; (2) Use in the preparation of products that enhance intestinal peristalsis; (3) Use in the preparation of products that increase the activity of protease, lactase, sucrase, aminopeptidase, maltase, cellulase and / or lipase; (4) Use in the degradation of proteins, lactose, peptides, maltose, cellulose and / or fat.

6. The use according to claim 5, characterized in that The products include food, health products or medicines; Optionally, the food includes beverages, milk powder, protein powder, complementary food, and fermented milk; the beverage includes solid beverage; the milk powder includes modulated milk powder; Optionally, the dosage form of the drug includes a liquid preparation, a semisolid preparation or a solid preparation; optionally, the dosage form of the drug includes a tablet, a powder, a drop, a granule, a capsule or an injection.

7. A method for preparing fermented milk that can promote nutrient digestion and absorption, characterized in that: The animal Bifidobacterium lactis subspecies B-01 described in claim 1 is used as the strain, and the inoculation amount is ≥1.0×10 7 CFU / g.

8. The method for preparing fermented milk capable of promoting nutrient digestion and absorption according to claim 7, characterized in that: Weigh 88-96 parts by weight of milk, preheat to 55-60°C, add 4-6 parts by weight of sugar, stir, rotate at 800-1000rpm, mix for 10-15min, homogenize at 60-65°C, primary pressure 20.0-30.0Mpa, secondary pressure 4.5-5.0Mpa, sterilize at 90-95°C, 5-10min, cool to 36-38°C for inoculation and fermentation, ferment for 12-14h until pH 4.4-4.6, cool and set aside.

9. Fermented milk prepared by the preparation method according to claim 7 or 8.

10. Use of the fermented milk according to claim 9 in any of the following items: (1) Use in the preparation of products that promote nutrition, digestion and absorption; (2) Use in the preparation of products that enhance intestinal peristalsis; Optionally, the product includes yogurt, flavored fermented milk, fermented buttermilk, yogurt wine or milk wine.

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