Method for synthesizing ratiometric fluorescent probe

Abstract

The invention specifically discloses a method for synthesizing a dual-emission wavelength fluorescent probe by using L-ascorbic acid. The method comprises the following steps: dissolving ascorbic acid in water and ethanol, carrying out ultrasonic treatment, uniformly mixing, putting into a reaction kettle, and carrying out high-temperature heating to finally obtain light yellow liquid. The prepared material is good in dispersity, the particle size is about 2 nm, and the material can be used for detecting an actual sample through a ratio fluorescent probe.

Description

Technical Field

[0001] The invention provides a method for synthesizing a ratio fluorescent probe using L-ascorbic acid as a raw material. The probe can be used for drug detection. Background Art

[0002] Fluorescent probes are a class of molecules with characteristic fluorescence. They are widely used in cell imaging, protein analysis, cell metabolism, etc. in the biomedical field. They are used to study the absorption and metabolism of drugs in the field of drug development. They are used to detect pollutants in the field of environmental monitoring: such as heavy metal ions, organic pollutants, pesticides, etc. In the field of chemical analysis, they are used to monitor organic molecules and analyze metal complexes. However, the synthesis and application process of traditional fluorescent probes has the disadvantages of cumbersome operation, easy contamination, only qualitative analysis, and certain toxicity. These disadvantages limit their application in the biomedical field and certain detection technologies. Therefore, researching and developing new fluorescent probes to improve their biocompatibility, quantum yield, stability, sensitivity and other properties is an important direction of current fluorescent probe research.

[0003] Common synthesis methods currently include high temperature pyrolysis, chemical reduction, sol method, hydrothermal synthesis, etc. Among them, the hydrothermal synthesis method has the advantages of high purity, environmental friendliness, high reaction activity, etc., so the use of hydrothermal synthesis to synthesize new fluorescent probes has important practical significance. The present invention uses L-ascorbic acid to synthesize a fluorescent probe with dual emission wavelengths in one step through a hydrothermal synthesis method, and constructs a probe system for quantitative detection of antibiotics in vitro, which opens up important new design ideas for the development of visualization, dynamic precision diagnosis and treatment. Summary of the invention

[0004] The vitamin-based fluorescent probe material synthesized by the present invention has dual fluorescence emission wavelengths at 440 nm and 530 nm. When the excitation and emission slits are both 5 nm, the excitation wavelength is 370 nm, and the concentration is 0.05 mol·L -1 When , the fluorescence intensities are 1507 nm and 2024 nm respectively.

[0005] In order to achieve the above-mentioned object and other related objects, the present invention provides a method for preparing a vitamin-based fluorescent probe by using L-ascorbic acid, and the specific steps are as follows: 0.11 g of L-ascorbic acid is dissolved in a mixed solution of 10 mL of water and 10 mL of ethanol, ultrasonicated for 3 min, mixed evenly, put into a reactor, heated at 140 ° C for 6 h, and finally obtained a light yellow liquid.

[0006] Therefore, the ultrasonic time is 1 to 10 min, and the preferred ultrasonic time is 3 min.

[0007] Therefore, the heating temperature is 0-150°C, and the preferred heating temperature is 140°C.

[0008] Therefore, when the heating time is 0 to 12 h, the preferred heating time is 6 h.

[0009] The present invention provides a method for synthesizing a vitamin-based fluorescent probe using L-ascorbic acid as a raw material, which has the characteristics of good selectivity, low cost and environmental protection. Through transmission electron microscopy, it can be seen that the synthetic material is well dispersed and the particle size is 2 nm. Through high-resolution transmission electron microscopy, it can be seen that the lattice spacing is 0.239 nm. The fluorescence emission spectrum shows that it has good ratio fluorescence performance. BRIEF DESCRIPTION OF THE DRAWINGS

[0010] Figure 1 Transmission electron microscopy image of the synthesized material.

[0011] Figure 2 XPS graph of the synthesized material.

[0012] Figure 3 The fluorescence spectrum of the synthesized material. DETAILED DESCRIPTION

[0013] The implementation method of the present invention is explained by specific examples, and the staff involved in the field of this invention can easily understand the advantages and effects of this invention through the contents explained in the specification. The present invention can also be implemented and applied through different implementation methods. Without violating the fundamentals of the present invention, different ideas and viewpoints can be applied to modify and improve the contents of this specification. The specific operation process is as follows:

[0014] Example 1: Synthesis of vitamin-based fluorescent probe using L-ascorbic acid. The specific method is as follows: 0.11 g of L-ascorbic acid is dissolved in a mixed solution of 10 ml of water and 10 ml of ethanol, and ultrasonicated for 3 min. After mixing evenly, the mixture is placed in a reactor and heated at 140°C for 6 hours to finally obtain a light yellow liquid.

[0015] Example 2: The above-synthesized materials were characterized by TEM. Figure 1 As shown in the XPS results, the synthesized material has good dispersibility, an average particle size of 2 nm, and a lattice spacing of 0.239 nm. Figure 2 The synthesized material shown is composed of C, H, and O elements, and has carboxyl and hydroxyl groups on the surface. Figure 3 ) It can be seen that the synthesized material has dual fluorescence emission wavelengths at 440 nm and 530 nm, and the concentration is 0.05 mol·L -1 The maximum fluorescence intensity is at 1507 nm and 2024 nm.

Claims

1. A method for synthesizing a dual-emission wavelength ratio fluorescent probe using L-ascorbic acid as a raw material, characterized in that Follow these steps: 0.11 g of L-ascorbic acid was dissolved in a mixed solution of 10 mL of water and 10 mL of ethanol, ultrasonicated for 3 min, mixed evenly, placed in a reactor, and heated at 140 °C for 6 h to finally obtain a light yellow liquid.

2. The synthesis method according to claim 1, wherein the mass of L-ascorbic acid is 0.11 g.

3. The synthesis method according to claim 1, wherein the ultrasonic time is 3 min.

4. The synthesis method according to claim 1, wherein the heating temperature is 140 °C.

5. The synthesis method according to claim 1, wherein the heating time is 6 h.

6. The synthesis method according to claim 1, wherein the material has dual fluorescence emission wavelengths at 440 nm and 530 nm, respectively, when the excitation wavelength is 370 nm and the concentration is 0.05 mol·L -1 The maximum fluorescence emission intensities were 1507 nm and 2024 nm, respectively.

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