Strain fermentation device

Abstract

The invention discloses a strain fermentation device which comprises a tank body, a sampling mechanism, a cleaning mechanism and a positioning mechanism, the sampling mechanism is arranged on the tank body, the cleaning mechanism is arranged on the sampling mechanism, the cleaning mechanism is used for cleaning the sampling mechanism, and the positioning mechanism is used for positioning in the sampling process of the sampling mechanism. The sampling mechanism comprises a liquid inlet nozzle, a liquid inlet pipe, a fixed cylinder, a liquid pumping cylinder, a piston and a liquid outlet nozzle, the liquid inlet nozzle is arranged in the tank body, the fixed cylinder is arranged on the tank body and penetrates through the tank body, the liquid pumping cylinder is rotationally arranged in the fixed cylinder, the outer circumferential surface of the liquid pumping cylinder is in contact with the inner circumferential surface of the fixed cylinder, and an outer liquid inlet is formed in the fixed cylinder. The invention relates to the technical field of strain fermentation, in particular to a strain fermentation device. The technical problem to be solved by the invention is to provide the strain fermentation device, so that fermentation liquor is not easy to remain in the liquid pumping cylinder, the probability of deterioration and pollution of the fermentation liquor is reduced, and the sampling accuracy is improved.

Description

Technical Field

[0001] The invention relates to the technical field of strain fermentation, in particular to a strain fermentation device. Background Art

[0002] During the fermentation process of the strain, it is necessary to open the tank storing the fermentation liquid at regular intervals and take samples of the fermentation liquid for testing, so as to timely observe factors such as the nutrients in the fermentation liquid, the growth of the strain, the accumulation of toxins, etc., thereby reducing the probability of the strain in the fermentation tank deteriorating.

[0003] When sampling the fermentation liquid in a traditional fermentation device, it is usually necessary to open the lid of the fermentation tank for sampling and testing. During sampling, bacteria and pollutants in the air can easily enter the tank, contaminating the fermentation liquid and causing the fermentation liquid to deteriorate.

[0004] At present, a Chinese invention patent with publication number CN113322163B proposes a beverage fermentation device, including a fermentation tank, a sampling mechanism is fixedly installed on the tank wall of the fermentation tank, the sampling mechanism includes a fixed cylinder, a liquid pumping cylinder, a piston, a piston rod and a liquid outlet nozzle. When sampling, the piston rod and the piston are rotated to drive the liquid pumping cylinder to rotate, so that the inner liquid inlet on the liquid pumping cylinder is connected with the outer liquid inlet on the fixed cylinder, and the piston is pulled to move by the piston rod, so that the liquid in the fermentation tank flows into the liquid pumping cylinder through the outer liquid inlet and the inner liquid inlet, and then the liquid pumping cylinder is rotated to make the inner liquid inlet connected with the liquid outlet channel, and then the piston is pushed to squeeze the liquid in the liquid pumping cylinder through the liquid outlet channel and the liquid outlet nozzle, so as to sample at the liquid outlet nozzle.

[0005] According to the above technical solution, after sampling, some fermentation liquid still remains in the liquid extraction tube, which is easy to deteriorate and adhere to the inner wall of the liquid extraction tube to cause pollution, thereby affecting the accuracy of sampling. Summary of the invention

[0006] The technical problem to be solved by the present invention is to provide a strain fermentation device, which makes it difficult for the fermentation liquid to remain in the liquid extraction cylinder, reduces the probability of the fermentation liquid being deteriorated and contaminated, and thus improves the accuracy of sampling.

[0007] The present invention adopts the following technical solutions to achieve the invention objectives:

[0008] A strain fermentation device comprises a tank body, a sampling mechanism and a cleaning mechanism, wherein the sampling mechanism is arranged on the tank body, the cleaning mechanism is arranged on the sampling mechanism, and the cleaning mechanism is used to clean the sampling mechanism;

[0009] The sampling mechanism comprises a liquid inlet nozzle, a liquid inlet tube, a fixed cylinder, a liquid extraction cylinder, a piston, and a liquid outlet nozzle, the liquid inlet nozzle is arranged in the tank body, the fixed cylinder is arranged on the tank body and passes through the tank body, the liquid extraction cylinder is rotatably arranged in the fixed cylinder, the outer circumference of the liquid extraction cylinder contacts the inner circumference of the fixed cylinder, the fixed cylinder is provided with an external liquid inlet, the liquid extraction cylinder is provided with an internal liquid inlet, the liquid inlet nozzle is communicated with the external liquid inlet through the liquid inlet tube, the piston is slidably arranged in the liquid extraction cylinder and contacts the inner circumference of the liquid extraction cylinder, one end of the piston passes through the liquid extraction cylinder and the fixed cylinder and extends to the outside of the fixed cylinder, the liquid outlet nozzle is arranged on the fixed cylinder, the fixed cylinder is provided with a liquid outlet channel, the internal liquid inlet can be communicated with the liquid outlet nozzle through the liquid outlet channel, the cleaning mechanism comprises a liquid injection nozzle and an ultrapure water machine, the liquid injection nozzle is arranged on the fixed cylinder, the fixed cylinder is provided with a liquid injection channel, the internal liquid inlet can be communicated with the liquid injection nozzle through the liquid injection channel, and the output end of the ultrapure water machine can be connected with the liquid injection nozzle.

[0010] As a further limitation of the present technical solution, the sampling mechanism further includes an electromagnetic one-way valve, and the electromagnetic one-way valve is arranged in the liquid inlet nozzle.

[0011] As a further limitation of the present technical solution, the liquid inlet pipe is an elastic pipe.

[0012] As a further limitation of the present technical solution, it also includes a positioning mechanism, which includes a ball, a spring, and a support rod. The support rod is inserted into the fixed tube and is slidably connected to the fixed tube. The ball is arranged on the support rod. The spring is sleeved on the support rod. A spherical groove is provided on the outer circumferential surface of the liquid extraction cylinder, and the ball abuts against the outer circumferential surface of the liquid extraction cylinder.

[0013] As a further limitation of the present technical solution, three spherical grooves are provided.

[0014] As a further limitation of the technical solution, the ball is rollingly connected to the support rod.

[0015] As a further limitation of the present technical solution, a chamfer is provided on the outer peripheral surface of the liquid pumping cylinder near the spherical groove.

[0016] As a further limitation of the present technical solution, the cleaning mechanism also includes a nitrogen purge device, and the output end of the nitrogen purge device can be connected to the liquid injection nozzle.

[0017] As a further limitation of the present technical solution, the sampling mechanism also includes a filter screen, and the filter screen is arranged on the liquid inlet nozzle.

[0018] As a further limitation of the present technical solution, the filter is tilted and forms an angle of 45 degrees with the horizontal plane.

[0019] Compared with the prior art, the advantages and positive effects of the present invention are:

[0020] 1. By setting up the cleaning mechanism, ultrapure water enters the liquid extraction cylinder through the liquid injection nozzle and the liquid injection channel to dilute the extracted fermentation liquid, thereby reducing the viscosity of the fermentation liquid and the probability of the fermentation liquid remaining in the liquid extraction cylinder, thereby reducing the probability of the deteriorated fermentation liquid contaminating the liquid extraction cylinder, and at the same time, the deterioration speed of the fermentation liquid after extraction is slowed down, thereby improving the accuracy and reliability of sampling and detection.

[0021] 2. Through the setting of the electromagnetic one-way valve and the liquid inlet pipe, after a certain amount of fermentation liquid is extracted, the electromagnetic one-way valve is closed to isolate the fermentation liquid outside the liquid inlet nozzle, and the piston is pulled to extract the residual fermentation liquid in the liquid inlet nozzle and the liquid inlet pipe, so that the liquid inlet pipe undergoes elastic deformation, thereby greatly reducing the residual amount of fermentation liquid in the liquid inlet nozzle and the liquid inlet pipe, and reducing the impact of deteriorated fermentation liquid on sampling accuracy.

[0022] 3. Through the setting of the positioning component, when the inner liquid inlet of the liquid extraction cylinder is aligned with the outer liquid inlet, liquid outlet channel or injection channel of the fixed cylinder, the ball enters the spherical groove along the chamfer under the action of the spring force, and the impact makes a sound to remind the rotation to be in place, which is convenient for accurately positioning the rotation angle of the liquid extraction cylinder, so that the fermentation liquid is not easy to remain on the outer wall of the liquid extraction cylinder, and it is not easy to cause pollution of the liquid extraction cylinder, thereby improving the accuracy of sampling. The setting of the chamfer makes the transition of the ball entering the spherical groove smooth. Since the ball is connected to the support rod by rolling, the wear of the ball and the outer wall of the liquid extraction cylinder is reduced, thereby improving the durability and reliability of the device.

[0023] 4. Through the setting of the nitrogen purge device, after the sampling is completed, the output end of the nitrogen purge device is connected to the liquid injection nozzle, the nitrogen purge device is turned on and the piston is pulled to allow the nitrogen to enter the liquid extraction cylinder through the liquid injection channel, and then the nitrogen purge device is closed, the piston is rotated to connect the inner liquid inlet of the liquid extraction cylinder with the liquid outlet channel, and the piston is pushed to discharge the nitrogen, so that the nitrogen carries the residual liquid and is discharged from the liquid outlet nozzle, thereby further cleaning the liquid extraction cylinder and the liquid outlet channel, reducing the interference of liquid residue on the sampling accuracy, and reducing the probability of fermentation liquid remaining in the liquid extraction cylinder, thereby improving the accuracy and reliability of sampling detection. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] The drawings described herein are used to provide a further understanding of the present application and constitute a part of the present application. The illustrative embodiments of the present application and their descriptions are used to explain the present application and do not constitute an improper limitation on the present application. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work. In the drawings:

[0025] Figure 1 A perspective view of the present invention;

[0026] Figure 2 for Figure 1 The cross-section along the AA direction;

[0027] Figure 3 for Figure 2 Enlarged view of part C;

[0028] Figure 4 for Figure 2 A magnified view of part D in the middle;

[0029] Figure 5 for Figure 1 The cross-section along the BB direction;

[0030] Figure 6 for Figure 5 Enlarged view of part E.

[0031] In the figure: 1. tank body; 2. sampling mechanism; 21. liquid inlet nozzle; 22. liquid inlet pipe; 23. fixing cylinder; 231. external liquid inlet; 232. liquid outlet channel; 233. liquid injection channel; 24. liquid extraction cylinder; 241. internal liquid inlet; 244. spherical groove; 25. piston; 26. liquid outlet nozzle; 27. electromagnetic one-way valve; 28. filter; 3. cleaning mechanism; 31. liquid injection nozzle; 32. ultrapure water machine; 33. nitrogen purge device; 4. positioning mechanism; 41. sphere; 42. spring; 43. support rod. DETAILED DESCRIPTION

[0032] The following will be combined with the attached embodiment of the present invention Figure 1-Figure 6 , the technical solutions in the embodiments of the present invention are clearly and completely described. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0033] It should be noted that the directional terms such as left, right, up, down, front and back in the embodiments of the present invention are merely relative concepts or are based on the normal use state of the product, that is, the direction of movement of the product, and should not be considered as limiting.

[0034] When a component is referred to as being “located” or “disposed on” another component, it may be on the other component or there may be an intervening component. When a component is referred to as being “connected to” another component, it may be directly connected to the other component or there may be an intervening component.

[0035] Embodiment: A strain fermentation device comprises a tank body 1, a sampling mechanism 2, a cleaning mechanism 3, and a positioning mechanism 4. The sampling mechanism 2 is arranged on the tank body 1, the cleaning mechanism 3 is arranged on the sampling mechanism 2, the cleaning mechanism 3 is used to clean the sampling mechanism 2, and the positioning mechanism 4 is used to position the sampling mechanism 2 during the sampling process.

[0036] The sampling mechanism 2 includes a liquid inlet nozzle 21, a liquid inlet tube 22, a fixed cylinder 23, a liquid extraction cylinder 24, a piston 25, a liquid outlet nozzle 26, an electromagnetic one-way valve 27, and a filter 28. The liquid inlet nozzle 21 is connected to the inner wall of the tank body 1, the electromagnetic one-way valve 27 is connected to the liquid inlet nozzle 21, the filter 28 is fixedly connected to the liquid inlet nozzle 21, the filter 28 is tilted and forms an angle of 45 degrees with the horizontal plane, the fixed cylinder 23 is fixedly connected to the tank body 1 and passes through the tank body 1, the liquid extraction cylinder 24 is rotatably connected to the fixed cylinder 23, and the outer circumferential surface of the liquid extraction cylinder 24 is in contact with the inner circumferential surface of the fixed cylinder 23, the contact is tight, and has a certain sealing effect.

[0037] The fixed cylinder 23 is provided with an external liquid inlet 231, and the liquid extraction cylinder 24 is provided with an internal liquid inlet 241. The liquid inlet nozzle 21 is connected with the external liquid inlet 231 through the liquid inlet tube 22. The liquid inlet tube 22 is an elastic tube. The external liquid inlet 231 can be connected with the internal liquid inlet 241. The outer peripheral surface of one end of the piston 25 contacts the inner peripheral surface of the liquid extraction cylinder 24. The other end of the piston 25 is in a cross-shaped structure and penetrates the liquid extraction cylinder 24 and the fixed cylinder 23, and extends to the outside of the fixed cylinder 23. A circular hole is provided at one end of the fixed cylinder 23 away from the external liquid inlet 231 for passing one end of the piston 25. A cross-shaped through hole is provided at one end of the liquid extraction cylinder 24 away from the internal liquid inlet 241 for passing one end of the piston 25. The piston 25 is slidably connected to the liquid extraction cylinder 24. The liquid outlet nozzle 26 is fixedly connected to the fixed cylinder 23. A liquid outlet channel 232 is provided on the fixed cylinder 23. The internal liquid inlet 241 can be connected to the liquid outlet nozzle 26 through the liquid outlet channel 232.

[0038] Specifically, the piston 25 is rotated to drive the liquid pumping cylinder 24 to rotate, so that the inner liquid inlet 241 of the liquid pumping cylinder 24 is aligned with the outer liquid inlet 231 of the fixed cylinder 23, so that the fermented liquid enters the liquid inlet pipe 22 through the liquid inlet nozzle 21, and the filter screen 28 blocks larger objects in the fermented liquid, so that the blocked objects slide down along the inclined filter screen 28, so that the filter screen 28 is not easy to be blocked, and then the liquid inlet nozzle 21 is blocked, and the piston 25 is pulled to allow the fermented liquid to enter the inside of the liquid pumping cylinder 24 through the outer liquid inlet 231 and the inner liquid inlet 241. The piston 25 is circumferentially fixed to the inner periphery of the liquid pumping cylinder 24, and the piston 25 can drive the liquid pumping cylinder 24 to rotate. Specifically, the outer periphery of the piston 25 is matched with the inner periphery of the liquid pumping cylinder 24 in a concave-convex manner. For example, the outer periphery of the piston 25 is provided with a strip protrusion, and the inner periphery of the liquid pumping cylinder 24 is provided with a strip groove for the strip protrusion to be embedded.

[0039] After a certain amount of fermentation liquid is extracted, the electromagnetic one-way valve 27 is closed to isolate the fermentation liquid outside the liquid inlet nozzle 21, and the piston 25 is pulled to extract the residual fermentation liquid in the liquid inlet nozzle 21 and the liquid inlet tube 22, so that the liquid inlet tube 22 undergoes elastic deformation, thereby greatly reducing the residual amount of the fermentation liquid and reducing the influence of the deterioration of the fermentation liquid on the sampling accuracy. The liquid extraction cylinder 24 is driven to rotate by rotating the piston 25, so that the inner liquid inlet 241 of the liquid extraction cylinder 24 is connected with the injection channel 233 of the fixed cylinder 23, and the ultrapure water machine 32 is turned on to supply water, so that ultrapure water enters the liquid extraction cylinder 24 through the injection channel 233, and the extracted fermentation liquid is diluted, thereby reducing the viscosity of the fermentation liquid and the probability of the fermentation liquid remaining in the liquid extraction cylinder 24. At the same time, the deterioration speed of the fermentation liquid after extraction is slowed down, thereby improving the accuracy and reliability of sampling detection;

[0040] The cleaning mechanism 3 includes a liquid injection nozzle 31, an ultrapure water machine 32, and a nitrogen purge device 33. The liquid injection nozzle 31 is fixedly connected to the fixed cylinder 23. The fixed cylinder 23 is provided with a liquid injection channel 233. The internal liquid inlet 241 can be connected to the liquid injection nozzle 31 through the liquid injection channel 233. The output end of the ultrapure water machine 32 can be connected to the liquid injection nozzle 31, and the output end of the nitrogen purge device 33 can be connected to the liquid injection nozzle 31.

[0041] Specifically, ultrapure water is allowed to enter the liquid extraction cylinder 24 through the liquid injection nozzle 31 and the liquid injection channel 233 to dilute the extracted fermentation liquid, thereby reducing the viscosity of the fermentation liquid and the probability of the fermentation liquid remaining in the liquid extraction cylinder 24, thereby reducing the probability of the deteriorated fermentation liquid contaminating the liquid extraction cylinder 24, and at the same time, the deterioration speed of the fermentation liquid after extraction is slowed down, thereby improving the accuracy and reliability of sampling detection. After the sampling is completed, the output end of the nitrogen purge device 33 is connected to the liquid injection nozzle 31, the nitrogen purge device 33 is turned on and the activity is pulled. Plug 25 is opened to allow nitrogen to enter the liquid extraction cylinder 24 through the injection channel 233, and then the nitrogen purging device 33 is closed. The piston 25 is rotated to connect the internal liquid inlet 241 of the liquid extraction cylinder 24 with the liquid outlet channel 232, and the piston 25 is pushed to discharge nitrogen, so that the nitrogen carries the residual liquid and is discharged from the liquid outlet nozzle 26, thereby further cleaning the liquid extraction cylinder 24 and the liquid outlet channel 232, reducing the interference of liquid residue on the sampling accuracy, reducing the probability of fermentation liquid remaining in the liquid extraction cylinder 24, and thus improving the accuracy and reliability of sampling detection.

[0042] The positioning mechanism 4 includes a ball 41, a spring 42, and a support rod 43. The support rod 43 is inserted into the fixed tube 23 and is slidably connected to the fixed tube 23. The ball 41 is rollingly connected to the support rod 43, and the spring 42 is sleeved on the support rod 43. Specifically, the support rod 43 includes a head portion whose upper end contacts the ball 41 and a rod portion connected to the lower end of the head portion. The spring 42 is sleeved on the rod portion. An L-shaped mounting plate 29 is fixedly provided on the outer peripheral wall of the fixed tube 23. The horizontal portion of the L-shaped mounting plate 29 is provided with a guide hole for the rod portion to be movably inserted. The lower end of the rod portion passes through the guide hole and is connected with a nut to achieve a movable travel limit. The upper end of the spring 42 contacts the head of the support rod 43, and the lower end contacts the L-shaped mounting plate 29, thereby achieving an elastic effect on the support rod 43.

[0043] The outer circumference of the liquid pumping cylinder 24 is provided with three spherical grooves 244, the sphere 41 abuts against the outer circumference of the liquid pumping cylinder 24, and the outer circumference of the liquid pumping cylinder 24 is provided with chamfers near the spherical grooves 244. The inner liquid inlet 241 has three states of being connected with the liquid outlet channel 232, the outer liquid inlet 231 and the liquid injection channel 233 respectively, and the sphere 41 is inserted into the three spherical grooves 244 corresponding to the three states of the inner liquid inlet 241.

[0044] Specifically, through the setting of the positioning component, when the inner liquid inlet 241 of the liquid extraction cylinder 24 is aligned with the outer liquid inlet 231, the liquid outlet channel 232 or the injection channel 233 of the fixed cylinder 23, the ball 41 enters the spherical groove 244 along the chamfer under the action of the elastic force of the spring 42, and the collision makes a sound to remind the rotation to be in place, which is convenient for accurately positioning the rotation angle of the liquid extraction cylinder 24, so that the fermentation liquid is not easy to remain on the outer wall of the liquid extraction cylinder 24, and it is not easy to cause contamination of the liquid extraction cylinder 24, thereby improving the accuracy of sampling. The setting of the chamfer makes the transition of the ball 41 entering the spherical groove 24 smooth. Since the ball 41 is rollingly connected to the support rod 43, the wear of the ball 41 and the outer wall of the liquid extraction cylinder 24 is reduced, thereby improving the durability and reliability of the device.

[0045] The working principle of this embodiment is:

[0046] When in use, the pumping cylinder 24 is driven to rotate by rotating the piston 25, so that the inner liquid inlet 241 of the pumping cylinder 24 is aligned with the outer liquid inlet 231 of the fixed cylinder 23. At this time, the ball 41 enters the spherical groove 244 along the chamfer under the action of the elastic force of the spring 42, and the collision makes a sound to remind the rotation to be in place, which is convenient for accurately positioning the rotation angle of the pumping cylinder 24, so that the fermentation liquid is not easy to remain on the outer wall of the pumping cylinder 24, thereby causing pollution to the outer wall of the pumping cylinder 24. The chamfered setting makes the transition of the ball 41 entering the spherical groove 244 smooth. 41 is connected to the support rod 43 in a rolling manner, which reduces the wear of the ball 41 and the outer wall of the liquid pumping cylinder 24. When the liquid pumping cylinder 24 rotates to a specified angle, the electromagnetic one-way valve 27 is opened, so that the fermentation liquid enters the liquid inlet pipe 22 through the liquid inlet nozzle 21, and the filter screen 28 blocks larger objects in the fermentation liquid, so that the blocked objects slide along the inclined filter screen 28, making the filter screen 28 less likely to be blocked, thereby blocking the liquid inlet nozzle 21, pulling the piston 25, and allowing the fermentation liquid to enter the interior of the liquid pumping cylinder 24 through the external liquid inlet port 231 and the internal liquid inlet port 241;

[0047] After a certain amount of fermentation liquid is extracted, the electromagnetic one-way valve 27 is closed to isolate the fermentation liquid outside the liquid inlet nozzle 21, and the piston 25 is pulled to extract the residual fermentation liquid in the liquid inlet nozzle 21 and the liquid inlet pipe 22, so that the liquid inlet pipe 22 undergoes elastic deformation, thereby greatly reducing the residual amount of fermentation liquid and reducing the impact of fermentation liquid deterioration on sampling accuracy. The output end of the ultrapure water machine 32 is connected to the liquid injection nozzle 31 through a pipeline, and the liquid extraction cylinder 24 is driven to rotate by rotating the piston 25, so that the liquid extraction cylinder 2 The inner liquid inlet 241 of the fixed cylinder 23 is connected with the injection channel 233 of the fixed cylinder 23, and the ball 41 hits to remind the rotation to the right position, and the ultrapure water machine 32 is turned on to supply water, so that the ultrapure water enters the liquid extraction cylinder 24 through the injection nozzle 31 and the injection channel 233, diluting the extracted fermentation liquid, thereby reducing the viscosity of the fermentation liquid, reducing the probability of the fermentation liquid remaining in the liquid extraction cylinder 24, and at the same time slowing down the deterioration speed of the fermentation liquid after extraction, thereby improving the accuracy and reliability of sampling detection;

[0048] After a certain amount of ultrapure water is added, the ultrapure water machine 32 is turned off, and the rotating piston 25 drives the liquid pumping cylinder 24 to rotate, so that the inner liquid inlet 241 is connected with the liquid outlet channel 232, and the ball 41 hits to remind the rotation to be in place, pushing the piston 25 to discharge the diluted fermentation liquid through the liquid outlet channel 232 and the liquid outlet nozzle 26, thereby sampling at the liquid outlet nozzle 26.

[0049] After sampling is completed, the piston 25 is rotated again to drive the liquid extraction cylinder 24 to rotate, so that the inner liquid inlet 241 is connected to the injection channel 233 again, the output end of the nitrogen purge device 33 is connected to the injection nozzle 31, the nitrogen purge device 33 is turned on and the piston 25 is pulled to allow nitrogen to enter the liquid extraction cylinder 24 through the injection channel 233, and then the nitrogen purge device 33 is closed, and the piston 25 is rotated to connect the inner liquid inlet 241 of the liquid extraction cylinder 24 with the liquid outlet channel 232, and the piston 25 is pushed to discharge nitrogen, so that the nitrogen carries the residual liquid and is discharged from the liquid outlet nozzle 26, thereby further cleaning the liquid extraction cylinder 24 and the liquid outlet channel 232, reducing the interference of liquid residue on the sampling accuracy, reducing the probability of fermentation liquid remaining in the liquid extraction cylinder 24, and thus improving the accuracy and reliability of sampling detection.

[0050] The above disclosure is only a specific embodiment of the present invention, but the present invention is not limited thereto, and any changes that can be conceived by those skilled in the art should fall within the protection scope of the present invention.

Claims

1. A bacterial strain fermentation device, characterized in that: It comprises a tank body (1), a sampling mechanism (2), and a cleaning mechanism (3), wherein the sampling mechanism (2) is arranged on the tank body (1), the cleaning mechanism (3) is arranged on the sampling mechanism (2), and the cleaning mechanism (3) is used to clean the sampling mechanism (2); The sampling mechanism (2) comprises a liquid inlet nozzle (21), a liquid inlet tube (22), a fixed cylinder (23), a liquid extraction cylinder (24), a piston (25), and a liquid outlet nozzle (26); the liquid inlet nozzle (21) is arranged in the tank body (1); the fixed cylinder (23) is arranged on the tank body (1) and penetrates the tank body (1); the liquid extraction cylinder (24) is rotatably arranged in the fixed cylinder (23); the outer peripheral surface of the liquid extraction cylinder (24) contacts the inner peripheral surface of the fixed cylinder (23); an outer liquid inlet (231) is provided on the fixed cylinder (23); an inner liquid inlet (241) is provided on the liquid extraction cylinder (24); the liquid inlet nozzle (21) is connected to the outer liquid inlet (231) through the liquid inlet tube (22); the piston (25) is slidably arranged in the liquid extraction cylinder (24) and is connected to the liquid extraction cylinder (26); 4) inner peripheral surface contact, one end of the piston (25) penetrates the liquid extraction cylinder (24) and the fixed cylinder (23) and extends to the outside of the fixed cylinder (23), the liquid outlet nozzle (26) is arranged on the fixed cylinder (23), a liquid outlet channel (232) is provided on the fixed cylinder (23), the inner liquid inlet (241) can be connected to the liquid outlet nozzle (26) through the liquid outlet channel (232), the cleaning mechanism (3) includes a liquid injection nozzle (31) and an ultrapure water machine (32), the liquid injection nozzle (31) is arranged on the fixed cylinder (23), a liquid injection channel (233) is provided on the fixed cylinder (23), the inner liquid inlet (241) can be connected to the liquid injection nozzle (31) through the liquid injection channel (233), and the output end of the ultrapure water machine (32) can be connected to the liquid injection nozzle (31).

2. A bacterial strain fermentation device according to claim 1, characterized in that: The sampling mechanism (2) further comprises an electromagnetic one-way valve (27), wherein the electromagnetic one-way valve (27) is arranged in the liquid inlet nozzle (21).

3. A bacterial strain fermentation device according to claim 2, characterized in that: The liquid inlet pipe (22) is an elastic pipe.

4. A bacterial strain fermentation device according to any one of claims 1 to 3, characterized in that: The invention also comprises a positioning mechanism (4), the positioning mechanism (4) comprising a ball (41), a spring (42), and a support rod (43), the support rod (43) being inserted into the fixed cylinder (23) and slidably connected to the fixed cylinder (23), the ball (41) being arranged on the support rod (43), the spring (42) being sleeved on the support rod (43), a spherical groove (244) being provided on the outer peripheral surface of the liquid extraction cylinder (24), and the ball (41) being in contact with the outer peripheral surface of the liquid extraction cylinder (24).

5. A bacterial strain fermentation device according to claim 4, characterized in that: Three spherical grooves (244) are provided.

6. A bacterial strain fermentation device according to claim 4, characterized in that: The ball (41) is rollingly connected to the support rod (43).

7. A bacterial strain fermentation device according to claim 4, characterized in that: The outer peripheral surface of the liquid extraction cylinder (24) is provided with a chamfer near the spherical groove (244).

8. A bacterial strain fermentation device according to claim 1, characterized in that: The cleaning mechanism (3) further comprises a nitrogen purge device (33), and the output end of the nitrogen purge device (33) can be connected to the liquid injection nozzle (31).

9. A bacterial strain fermentation device according to claim 1, characterized in that: The sampling mechanism (2) further comprises a filter screen (28), wherein the filter screen (28) is arranged on the liquid inlet nozzle (21).

10. A bacterial strain fermentation device according to claim 9, characterized in that: The filter screen (28) is arranged at an angle of 45 degrees to the horizontal plane.

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