Method for improving quality and efficiency of drought alkali wheat by microalgae

Abstract

The invention relates to the field of agriculture, in particular to a method for improving the quality and efficiency of Alkaline wheat by microalgae, which comprises the following steps: step S100, screening out a microalgae variety which adapts to the growth environment of Alkaline wheat and has strong salt tolerance and drought tolerance; step S200, culturing the obtained microalgae variety which adapts to the growth environment of the dry alkaline wheat and is high in salt tolerance and drought tolerance, and preparing the cultured microalgae into a liquid microalgae preparation; s300, the microalgae preparation is applied in a soil surface spraying and root irrigation mode; after the microalgae preparation is applied through soil surface spraying and root irrigation, the protein content of the drought alkali wheat is averagely increased by 5%-7%, the nutritional quality is remarkably improved, the soil organic matter content is increased by 27%-35%, and then the microalgae preparation is used for improving the quality and efficiency of the drought alkali wheat.

Description

Technical Field

[0001] The invention relates to the field of agriculture, and in particular to a method for improving the quality and efficiency of drought-resistant alkali wheat using microalgae. Background Art

[0002] Alkali wheat is a crop that grows under specific arid and saline conditions, and its yield and quality are limited by the growing environment. Traditional agricultural methods of increasing yield and improving quality have shown certain limitations in the field of alkali wheat cultivation, specifically high costs and potential adverse effects on the environment. In view of this, it is urgent to develop a novel, environmentally friendly and low-cost method to optimize the growth conditions of alkali wheat, thereby improving its yield and quality. Summary of the invention

[0003] The invention provides a method for improving the quality and efficiency of alkali-alkali wheat by using microalgae, and the technical problem to be solved is: how to improve the yield and quality of alkali-alkali wheat.

[0004] In order to achieve the above-mentioned object of the invention, the technical solution adopted by the present invention is:

[0005] A method for improving the quality and efficiency of dry alkali wheat with microalgae, comprising the following steps:

[0006] Step S100, screening out microalgae varieties that are adapted to the growth environment of drought-resistant wheat and have strong salt and drought tolerance;

[0007] Step S200, cultivating the obtained microalgae varieties that are adapted to the growth environment of drought-resistant wheat and have strong salt and drought tolerance, and preparing the cultivated microalgae into a liquid microalgae preparation;

[0008] Step S300, applying the microalgae preparation by spraying on the soil surface and irrigating the roots; after applying the microalgae preparation by spraying on the soil surface and irrigating the roots, the protein content of the dry alkali wheat is increased by an average of 5% to 7%, the nutritional quality is significantly improved, and the soil organic matter content is increased by 27% to 35%.

[0009] Further, step S100 includes S110 and S120;

[0010] Step S110, collecting microalgae samples from natural areas similar to the growth environment of the alkali wheat; the natural areas include waters near saline-alkali land, salt lakes, and seashore beaches;

[0011] Step S200, inoculate the collected different microalgae into a culture medium simulating the growth environment of alkali wheat. The salinity and drought degree of the culture medium should be the same as the soil conditions of the alkali wheat growth area. Regularly measure the growth of the microalgae and calculate its growth rate. Microalgae varieties with faster growth rates are more adapted to the growth environment of alkali wheat.

[0012] Further, specifically, step S200 includes S210 and S220;

[0013] Step S210, culturing method: inoculating the screened microalgae into a culture medium and placing it in a culture environment where the temperature and light can be strictly controlled;

[0014] Step 220, harvesting method: using a centrifuge to separate the microalgae from the culture solution; generally, a relative centrifugal force (RCF) of 1000-3000 g is used, and the centrifugation time is 10-20 minutes;

[0015] Step 230, preparing microalgae concentrate: using a disc centrifuge to collect microalgae to prepare microalgae concentrate;

[0016] Step S240, mixing and homogenizing: Use a stirrer or homogenizer to fully mix the preparation; the stirring speed can be controlled at 100-500rpm, and the homogenization pressure can be set at 4-12MPa to ensure that the microalgae cells or their active ingredients are evenly distributed in the solution to form a stable liquid microalgae improved preparation.

[0017] Further, in step S210, the culture conditions are: the temperature is controlled between 15-30°C, the light intensity is set between 5000-10000 lux, and the light cycle is 12-16 hours light / 8-12 hours dark.

[0018] Furthermore, the growth of microalgae was regularly monitored by cell counting and other methods. When the number of microalgae reached 3×10 7 cells / mL-5×10 7 When the number of cells / mL reached 1,000, the culture was stopped.

[0019] Further, in step S2320, specifically, the concentration is performed using a disc centrifuge at 12000-14000 rpm, a concentration time of 7-10 min, and a liquid discharge time of 8-10 seconds, and the concentration multiple is 200-300 times.

[0020] Furthermore, auxiliary ingredients are added, including beneficial soil bacteria (200-500 million / mL), organic matter (80-100 g / L), and preservatives to enhance the function and shelf life of the preparation.

[0021] The beneficial effects of the present invention are:

[0022] (1) Improve soil environment: Microalgae can increase soil organic matter content during their growth and reproduction in the soil. After microalgae preparations are applied by spraying on the soil surface and irrigating the roots, the soil organic matter content increases by 27%-35%. Organic matter is an important component of soil fertility. It can improve soil structure, make the soil more loose and breathable, and is conducive to the growth and respiration of the roots of drought-resistant alkali wheat, and enhance the root system's ability to absorb nutrients and water.

[0023] (2) At the same time, organic matter can also provide carbon sources and energy for microorganisms in the soil, promote the activity of soil microorganisms, further decompose and transform nutrients in the soil, make them easier for the wheat to absorb and utilize, provide more sufficient nutrition for the growth of the wheat, and help improve the yield and quality of the wheat.

[0024] (3) In addition, microalgae may secrete some plant growth regulators during their growth process, such as auxin and cytokinin. These substances can regulate the growth and development of R. alkali wheat, promote cell division, elongation and differentiation, improve the photosynthesis efficiency of R. alkali wheat, and enhance its resistance to environmental stress, thereby helping to improve the yield and quality of R. alkali wheat.

[0025] (4) This method is low-cost, environmentally friendly and sustainable, providing a strong guarantee for improving the quality and efficiency of dry alkali wheat. After a large number of experimental studies, the yield of dry alkali wheat increased by an average of 31.2% to 39.34% after using this method. Comparative tests showed that when microalgae preparations were not used, the per-acre yield of dry alkali wheat was 123.8-128.6 kg, and after using this method, it could reach 162.4-179.2 kg. DETAILED DESCRIPTION

[0026] In order to make the contents of the present invention more clearly understood, the technical solutions in the embodiments of the present invention are described clearly and completely.

[0027] A method for improving the quality and efficiency of dry alkali wheat with microalgae, comprising the following steps:

[0028] Step S100, screening out microalgae varieties that are adapted to the growth environment of drought-resistant wheat and have strong salt and drought tolerance.

[0029] Specifically, step S100 includes S110 and S120; step S110, collecting microalgae samples from natural areas similar to the growth environment of alkali wheat, such as waters near saline-alkali land, salt lakes, and seashore beaches. The microalgae in these places have lived in high-salt, drought or similar environments for a long time, and may have the potential to adapt to the growth environment of alkali wheat. Step S200, inoculate the collected different microalgae into a culture medium that simulates the growth environment of alkali wheat. The salinity and drought degree of the culture medium should be the same as the soil conditions of the alkali wheat growth area. The growth of the microalgae is measured regularly, and its growth rate is calculated. The microalgae varieties with faster growth rates are more adapted to the growth environment of alkali wheat.

[0030] Step S200: Cultivate the obtained microalgae varieties that are adapted to the growth environment of drought-resistant wheat and have strong salt and drought resistance, and prepare the cultivated microalgae into a liquid microalgae preparation.

[0031] Specifically, step S200 includes S210 and S220;

[0032] Step S210, culture method: inoculate the selected microalgae into the culture medium and place it in a culture environment where the temperature and light can be strictly controlled; wherein the temperature is controlled between 15-30°C, the light intensity is set between 5000-10000 lux, and the light cycle is 12-16 hours of light / 8-12 hours of darkness. Regularly monitor the growth of microalgae, and use methods such as cell counting to determine the number of microalgae when the number reaches 3×10 per liter. 7 cells / mL-5×10 7 When the number of cells / mL reached 1,000, the culture was stopped.

[0033] Step 220, harvesting method: use a centrifuge to separate the microalgae from the culture solution. Generally, a relative centrifugal force (RCF) of 1000-3000 g is used, and the centrifugation time is 10-20 minutes.

[0034] Step 230, preparing microalgae concentrate: using a disc centrifuge to collect the microalgae culture, using a disc centrifuge at 12000-14000rpm, concentration time 7-10min, liquid discharge time 8-10 seconds, concentration multiple 200-300 times. Auxiliary ingredients can also be added, including soil beneficial bacteria (200-500 million / mL), organic matter (80-100g / L), preservatives, to enhance the function and shelf life of the preparation.

[0035] Step S240, mixing and homogenizing: Use a stirrer or homogenizer to fully mix the preparation. The stirring speed can be controlled at 100-500rpm, and the homogenization pressure can be set at 4-12MPa to ensure that the microalgae cells or their active ingredients are evenly distributed in the solution to form a stable liquid microalgae improved preparation.

[0036] Step S300, applying the microalgae preparation by spraying on the soil surface and irrigating the roots. After applying the microalgae preparation by spraying on the soil surface and irrigating the roots, the protein content of the dry alkali wheat increased by an average of 5% to 7%, the nutritional quality was significantly improved, and the soil organic matter content increased by 27% to 35%.

[0037] The principle of this program to improve the yield and quality of drought-resistant alkali wheat is mainly reflected in the following aspects:

[0038] (1) Improve soil environment: Microalgae can increase soil organic matter content during their growth and reproduction in the soil. After microalgae preparations are applied by spraying on the soil surface and irrigating the roots, the soil organic matter content increases by 27%-35%. Organic matter is an important component of soil fertility. It can improve soil structure, make the soil more loose and breathable, and is conducive to the growth and respiration of the roots of drought-resistant alkali wheat, and enhance the root system's ability to absorb nutrients and water.

[0039] (2) At the same time, organic matter can also provide carbon sources and energy for microorganisms in the soil, promote the activity of soil microorganisms, further decompose and transform nutrients in the soil, make them easier for the wheat to absorb and utilize, provide more sufficient nutrition for the growth of the wheat, and help improve the yield and quality of the wheat.

[0040] (3) In addition, microalgae may secrete some plant growth regulators during their growth process, such as auxin and cytokinin. These substances can regulate the growth and development of R. alkali wheat, promote cell division, elongation and differentiation, improve the photosynthesis efficiency of R. alkali wheat, and enhance its resistance to environmental stress, thereby helping to improve the yield and quality of R. alkali wheat.

[0041] In summary, this method is low-cost, environmentally friendly and sustainable, and provides a strong guarantee for improving the quality and efficiency of dry alkali wheat. After a large number of experimental studies, the yield of dry alkali wheat increased by an average of 31.2% to 39.34% after using this method. Comparative experiments showed that when microalgae preparations were not used, the yield of dry alkali wheat per mu was 123.8-128.6 kg, and after using this method, it could reach 162.4-179.2 kg.

[0042] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention shall be included in the protection scope of the present invention.

Claims

1. A method for improving the quality and efficiency of dry alkali wheat with microalgae, characterized in that: The following steps are involved: Step S100, screening out microalgae varieties that are adapted to the growth environment of drought-resistant wheat and have strong salt and drought tolerance; Step S200, cultivating the obtained microalgae varieties that are adapted to the growth environment of drought-resistant wheat and have strong salt and drought tolerance, and preparing the cultivated microalgae into a liquid microalgae preparation; Step S300: applying the microalgae preparation by spraying on the soil surface and irrigating the roots.

2. The method for improving the quality and efficiency of dry alkali wheat by microalgae according to claim 1, characterized in that: Step S100 includes S110 and S120; Step S110, collecting microalgae samples from natural areas similar to the growth environment of the alkali wheat; the natural areas include waters near saline-alkali land, salt lakes, and seashore beaches; Step S200, inoculate the collected different microalgae into a culture medium simulating the growth environment of alkali wheat. The salinity and drought degree of the culture medium should be the same as the soil conditions of the alkali wheat growth area. Regularly measure the growth of the microalgae and calculate its growth rate. Microalgae varieties with faster growth rates are more adapted to the growth environment of alkali wheat.

3. The method for improving the quality and efficiency of dry alkali wheat by microalgae according to claim 1, characterized in that: Specifically, step S200 includes S210 and S220; Step S210, culturing method: inoculating the screened microalgae into a culture medium and placing it in a culture environment where the temperature and light can be strictly controlled; Step 220, harvesting method: using a centrifuge to separate the microalgae from the culture solution; generally, a relative centrifugal force (RCF) of 1000-3000 g is used, and the centrifugation time is 10-20 minutes; Step 230, preparing microalgae concentrate: using a disc centrifuge to collect microalgae to prepare microalgae concentrate; Step S240, mixing and homogenizing: Use a stirrer or homogenizer to fully mix the preparation; the stirring speed can be controlled at 100-500rpm, and the homogenization pressure can be set at 4-12MPa to ensure that the microalgae cells or their active ingredients are evenly distributed in the solution to form a stable liquid microalgae improved preparation.

4. The method for improving the quality and efficiency of dry alkali wheat by microalgae according to claim 3, characterized in that: In step S210, the culture conditions are: the temperature is controlled between 15-30°C, the light intensity is set between 5000-10000 lux, and the light cycle is 12-16 hours light / 8-12 hours dark.

5. The method for improving the quality and efficiency of dry alkali wheat by microalgae according to claim 4, characterized in that: The growth of microalgae was regularly monitored by cell counting and other methods. When the number of microalgae reached 3×10 per liter, 7 cells / mL-5×10 7 When the number of cells / mL reached 1,000, the culture was stopped.

6. The method for improving the quality and efficiency of dry alkali wheat by microalgae according to claim 3, characterized in that: In step S2320, specifically, the concentration is performed using a disc centrifuge at 12000-14000 rpm, a concentration time of 7-10 min, and a liquid discharge time of 8-10 seconds, and the concentration multiple is 200-300 times.

7. The method for improving the quality and efficiency of dry alkali wheat by microalgae according to claim 6, characterized in that: Auxiliary ingredients are also added, including beneficial soil bacteria (200-500 million / mL), organic matter (80-100 g / L), and preservatives to enhance the function and shelf life of the preparation.