Skin care essential oil for delaying cell senescence and removing acnes based on waxberry kernel flavone

Abstract

The invention relates to the technical field of skin care essential oil preparation, and discloses waxberry kernel flavone-based skin care essential oil for delaying cell aging and removing acnes, which is prepared from 89% of waxberry kernel flavone extract, 5% of ganoderma lucidum polysaccharide extract, 2% of penetration enhancer, 0.5% of hyaluronic acid, 3% of lecithin and 0.5% of preservative phenoxyethanol. According to the skincare essential oil for delaying cell aging and removing acnes based on waxberry kernel flavone, impurities are removed and the waxberry kernel flavone is extracted through a supercritical carbon dioxide extraction method, the efficient and selective extraction process is achieved, the extraction rate of flavonoid compounds is increased, it is guaranteed that the biological activity of the flavonoid compounds is not damaged, and the skincare essential oil is suitable for being used as a skincare essential oil for delaying cell aging and removing acnes. According to the ganoderma lucidum polysaccharide powder and the preparation method thereof, a high-quality base material is provided for preparation of subsequent skin care products, a high-purity ganoderma lucidum polysaccharide extract is obtained through the steps of drying, smashing, hot water extraction, centrifugal separation, ethanol precipitation, dialysis freeze drying and the like on ganoderma lucidum sporocarp, and the finally obtained ganoderma lucidum polysaccharide powder finished product has extremely high purity and stability.

Description

Technical Field

[0001] The invention relates to the technical field of preparation of skin care essential oils, in particular to skin care essential oils based on bayberry kernel flavonoids for delaying cell aging and removing acne. Background Art

[0002] The preparation technology of skin care essential oil refers to the process of combining a variety of natural plant extracts, active ingredients and other auxiliary materials in a specific proportion and process flow to form a skin care product with specific functions (such as anti-aging, moisturizing, anti-oxidation, etc.). This process involves multiple steps and technical means to ensure that the final product not only has an efficient skin care effect, but also provides a good user experience and safety. However, in the existing technology, the extraction method of bayberry kernel flavonoids and ganoderma lucidum polysaccharides is not efficient enough, resulting in a low content of effective ingredients, and the active ingredients in existing skin care products are easily affected by the external environment (such as light, temperature) and degraded, affecting the product effect. At the same time, the large molecular weight active ingredients in many skin care products are difficult to penetrate the stratum corneum, resulting in poor absorption effect. Secondly, the existing skin care products use a fixed formula ratio and cannot be adjusted according to individual differences. The antioxidant ingredients of some skin care products are single and the effect is limited. Summary of the invention

[0003] The purpose of this section is to summarize some aspects of embodiments of the present invention and briefly introduce some preferred embodiments. Some simplifications or omissions may be made in this section and the specification abstract and the invention title of this application to avoid blurring the purpose of this section, the specification abstract and the invention title, and such simplifications or omissions cannot be used to limit the scope of the present invention.

[0004] To achieve the above object, the present invention provides the following technical solutions:

[0005] A skin care essential oil based on bayberry kernel flavonoids for delaying cell aging and removing acne, comprising: the skin care essential oil is made of 89% bayberry kernel flavonoids extract, 5% ganoderma lucidum polysaccharide extract, 2% penetration enhancer, 0.5% hyaluronic acid, 3% lecithin, and 0.5% preservative phenoxyethanol;

[0006] removing impurities from bayberry core flavonoids, extracting bayberry core flavonoids by supercritical carbon dioxide extraction to obtain bayberry core flavonoids extract, removing the solvent by rotary evaporator, and separating the target compound by column chromatography purification technology to obtain bayberry core flavonoids extract;

[0007] The ganoderma lucidum fruiting body is dried and then crushed, extracted by hot water extraction, the obtained extract is centrifuged to separate the solid residue, ethanol is added to precipitate the polysaccharide, the precipitate is collected and small molecular impurities are removed by dialysis bag, and freeze-dried to prepare a powdered finished product to obtain a ganoderma lucidum polysaccharide extract;

[0008] The obtained bayberry kernel flavonoids extract and ganoderma lucidum polysaccharide extract were used to prepare hollow liposomes by thin film dispersion method, construct a microemulsion system, and form encapsulated nanoparticles.

[0009] Based on the microemulsion system, a synthetic penetration enhancer is added, the ratio of the constructed microemulsion system is adjusted, the ratio of bayberry kernel flavonoids and ganoderma lucidum polysaccharide is determined, and the skin care essential oil is prepared according to the determined ratio to obtain.

[0010] As a further solution of the present invention: the steps of removing impurities from bayberry core flavonoids and extracting bayberry core flavonoids by supercritical carbon dioxide extraction to obtain bayberry core flavonoids extract are as follows:

[0011] The dried bayberry pits are cleaned to remove impurities and surface dirt, and the cleaned bayberry pits are broken into small pieces;

[0012] The crushed bayberry kernels were placed in a supercritical carbon dioxide extraction device, and the extraction parameters were set to 30 MPa and 40° C., and the extraction was performed to collect the extract containing flavonoid compounds;

[0013] The extraction conditions were optimized during the extraction process, and the expression is:

[0014]

[0015] Wherein, E is the extraction efficiency, P is the system pressure, which indicates the pressure applied during the extraction process, T is the temperature, which indicates the temperature during the extraction process, C is the diffusion coefficient, which represents the diffusion rate of the target component in SC-CO2, C is the concentration factor, and H is the polarity index of the target component;

[0016] The bayberry kernel flavonoids extract is obtained.

[0017] As a further solution of the present invention: the solvent is removed by a rotary evaporator, and the target compound is separated by column chromatography purification technology to obtain the bayberry kernel flavonoid extract, and the specific steps are:

[0018] The carbon dioxide solvent used in the extraction process is removed under reduced pressure using a rotary evaporator, and a concentrated solution containing flavonoids is retained;

[0019] The concentrate was purified by silica gel column chromatography using ethyl acetate-petroleum ether as eluent;

[0020] The eluate is collected and detected by thin layer chromatography to obtain a high-purity bayberry kernel flavonoid extract.

[0021] As a further solution of the present invention: the Ganoderma lucidum fruiting body is dried and then crushed, extracted by hot water extraction method, and the obtained extract is centrifuged to separate the solid residue, the specific steps are:

[0022] Place fresh Ganoderma lucidum fruiting bodies in a forced air drying oven and dry them to constant weight at 40°C, ensuring that the moisture content is less than 10%;

[0023] Use a high-speed grinder to grind the dried Ganoderma lucidum fruiting bodies into fine powder and pass through an 80-mesh sieve;

[0024] Add the crushed Ganoderma lucidum powder into deionized water at a ratio of 1:10;

[0025] Hot water extraction was performed at 85°C, and the duration was set to 2 hours;

[0026] After the extraction, the mixture was centrifuged at 3000 rpm for 15 minutes to separate the solid residue, and the supernatant was collected as the crude polysaccharide extract.

[0027] As a further solution of the present invention, the steps of adding ethanol to precipitate the polysaccharide, collecting the precipitate and removing small molecule impurities through a dialysis bag, and freeze-drying the precipitate to prepare a powdery finished product to obtain a Ganoderma lucidum polysaccharide extract are as follows:

[0028] Slowly add 4 times the volume of 95% ethanol to the collected supernatant, stir evenly and let stand overnight to allow the polysaccharide to fully precipitate;

[0029] The precipitate was collected by centrifugation and washed several times with anhydrous ethanol to remove residual small molecule impurities;

[0030] The precipitate was placed in a dialysis bag and dialyzed with deionized water for 48 hours, with the dialysate replaced every 12 hours to completely remove small molecule impurities;

[0031] Finally, the dialyzed sample is freeze-dried to obtain a finished product of Ganoderma lucidum polysaccharide powder, which is Ganoderma lucidum polysaccharide extract.

[0032] As a further solution of the present invention: the obtained bayberry kernel flavonoids extract and ganoderma lucidum polysaccharide extract are used to prepare hollow liposomes by thin film dispersion method, and the specific steps are as follows:

[0033] Dissolve the bayberry fruit core flavonoids extract and ganoderma lucidum polysaccharide extract in an appropriate amount of water;

[0034] Prepare lecithin as membrane material, dissolve lecithin in anhydrous ethanol to make a solution with a concentration of 10 mg / mL;

[0035] The ethanol was evaporated using a rotary evaporator at 40 °C under reduced pressure, so that the lipids were deposited on the container wall to form a uniform film;

[0036] adding an aqueous solution containing bayberry kernel flavonoids and ganoderma lucidum polysaccharide to the formed lipid film, and performing hydration treatment under gentle stirring to redisperse the lipid film to form hollow liposomes;

[0037] Ultrasonic treatment was used to refine the particle size of hollow liposomes so that the average diameter of hollow liposomes was about 100 nm.

[0038] As a further solution of the present invention: the microemulsion system is constructed to form encapsulated nanoparticles, and the specific steps are:

[0039] The surfactant lecithin is used to reduce the interfacial tension and promote stable mixing between the oil and water phases;

[0040] The water phase and the oil phase were mixed under high-speed shear conditions and processed using a homogenizer at a speed of 10,000 rpm for 10 minutes to form a preliminary water-in-oil microemulsion;

[0041] The particle size distribution of the microemulsion is detected by dynamic light scattering method, so that the average particle size is between 100-200nm, forming encapsulated nanoparticles.

[0042] As a further solution of the present invention: in the process of constructing the water-in-oil microemulsion system, a formula for optimizing the performance of the microemulsion system is introduced, which is as follows:

[0043] Optimize the performance of the microemulsion system and select the optimal liposome size and microemulsion stability. The expression is:

[0044]

[0045] Among them, S is the stability index, which indicates the long-term stability of the microemulsion system, L is the liposome particle size, V is the volume ratio, C is the concentration factor, D is the diffusion coefficient, and H is the interfacial tension.

[0046] As a further solution of the present invention: Based on the microemulsion system, a synthetic penetration enhancer is added, the ratio of the constructed microemulsion system is adjusted, and the ratio of the bayberry kernel flavonoids and the ganoderma lucidum polysaccharide is determined, and the specific steps are:

[0047] Menthol derivatives were selected as synthetic penetration enhancers to open the stratum corneum gap;

[0048] Add menthol derivatives to the constructed microemulsion system according to the formula ratio and stir thoroughly;

[0049] Use a high-precision balance to accurately weigh the mass of each ingredient and mix them according to the formula ratio;

[0050] During the mixing process, the physical properties, viscosity and appearance of the system are continuously monitored to ensure uniform mixing and no stratification.

[0051] As a further solution of the present invention: in the process of determining the ratio of bayberry kernel flavonoids to ganoderma lucidum polysaccharide, an optimization formula is introduced, and the expression is:

[0052]

[0053] Among them, O is the optimization index, which represents the optimal ratio of bayberry kernel flavonoids and Ganoderma lucidum polysaccharides, F is the flavonoid concentration, P is the polysaccharide concentration, A is the activity factor, B is the permeability coefficient, and C is the carrier effect.

[0054] Compared with the prior art, the present invention has the following beneficial effects:

[0055] Impurities were removed and flavonoids from bayberry kernels were extracted by supercritical carbon dioxide extraction, achieving an efficient and selective extraction process, which not only improved the extraction rate of flavonoid compounds, but also ensured that their biological activity was not damaged, providing high-quality basic materials for the preparation of subsequent skin care products. High-purity ganoderma lucidum polysaccharide extracts were obtained by drying, crushing, hot water extraction, centrifugal separation, ethanol precipitation, dialysis freeze drying and other steps of ganoderma lucidum fruiting bodies. The final ganoderma lucidum polysaccharide powder product had extremely high purity and stability, which enhanced the overall anti-aging effect of skin care essential oils and provided a guarantee for the safety and effectiveness of the products. Hollow liposomes were prepared by using a thin film dispersion method on the obtained bayberry kernel flavonoids extract and ganoderma lucidum polysaccharide extract, and a microemulsion system was constructed to achieve effective encapsulation of active ingredients, which not only protected the active ingredients from the influence of the external environment, but also significantly improved their skin permeability and absorption efficiency, thereby enhancing the efficacy of skin care essential oils and providing a more lasting anti-aging effect. The active ingredients in bayberry kernel flavonoids and ganoderma lucidum polysaccharides can effectively inhibit the formation of acne, reduce the frequency of acne, and accelerate the healing of existing acne. BRIEF DESCRIPTION OF THE DRAWINGS

[0056] Figure 1 The present invention is a flow chart of a method for producing skin care essential oil based on bayberry kernel flavonoids to delay cell aging and remove acne. DETAILED DESCRIPTION

[0057] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and understandable, the specific implementation modes of the present invention are described in detail below in conjunction with the accompanying drawings.

[0058] In the following description, many specific details are set forth to facilitate a full understanding of the present invention, but the present invention may also be implemented in other ways different from those described herein, and those skilled in the art may make similar generalizations without violating the connotation of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.

[0059] Secondly, the term "one embodiment" or "embodiment" as used herein refers to a specific feature, structure, or characteristic that may be included in at least one implementation of the present invention. The term "in one embodiment" that appears in different places in this specification does not necessarily refer to the same embodiment, nor does it refer to a separate or selective embodiment that is mutually exclusive with other embodiments.

[0060] Example 1

[0061] See also Figure 1 , which is the first embodiment of the present invention, provides a skin care essential oil based on bayberry kernel flavonoids for delaying cell aging and removing acne, comprising:

[0062] The skin care essential oil is made of 89% bayberry fruit core flavonoids extract, 5% ganoderma lucidum polysaccharide extract, 2% penetration enhancer, 0.5% hyaluronic acid, 3% lecithin, and 0.5% preservative phenoxyethanol.

[0063] S1. removing impurities from bayberry core flavonoids, extracting bayberry core flavonoids by supercritical carbon dioxide extraction to obtain bayberry core flavonoids extract, removing the solvent by rotary evaporator, and separating the target compound by column chromatography purification technology to obtain bayberry core flavonoids extract;

[0064] Furthermore, the impurities of the bayberry core flavonoids are removed, and the bayberry core flavonoids are extracted by supercritical carbon dioxide extraction to obtain the bayberry core flavonoids extract, and the specific steps are as follows:

[0065] The dried bayberry pits are cleaned to remove impurities and surface dirt, and the cleaned bayberry pits are broken into small pieces;

[0066] The crushed bayberry kernels were placed in a supercritical carbon dioxide extraction device, and the extraction parameters were set to 30 MPa and 40° C., and the extraction was performed to collect the extract containing flavonoid compounds;

[0067] The extraction conditions were optimized during the extraction process, and the expression is:

[0068]

[0069] Wherein, E is the extraction efficiency, P is the system pressure, which indicates the pressure applied during the extraction process, T is the temperature, which indicates the temperature during the extraction process, D is the diffusion coefficient, which represents the diffusion rate of the target component in SC-CO2, C is the concentration factor, and H is the polarity index of the target component;

[0070] Obtaining the bayberry kernel flavonoids extract;

[0071] The solvent was removed by a rotary evaporator, and the target compound was separated by column chromatography purification technology to obtain the bayberry kernel flavonoid extract. The specific steps are as follows:

[0072] The carbon dioxide solvent used in the extraction process is removed under reduced pressure using a rotary evaporator, and a concentrated solution containing flavonoids is retained;

[0073] The concentrate was purified by silica gel column chromatography using ethyl acetate-petroleum ether as eluent;

[0074] The eluate is collected and detected by thin layer chromatography to obtain a high-purity bayberry kernel flavonoid extract.

[0075] It should be noted that the extraction of bayberry fruit core flavonoids by supercritical carbon dioxide extraction can not only efficiently remove impurities and retain biologically active ingredients, but also ensure operation under low temperature conditions to protect heat-sensitive substances. In addition, by optimizing the extraction conditions, especially the precise control of system pressure and temperature parameters (30MPa, 40°C), and introducing variables such as diffusion coefficient and concentration factor for mathematical modeling, the extraction efficiency is further improved, ensuring that the final bayberry fruit core flavonoids extract has high purity and high activity, laying a solid foundation for the subsequent preparation of skin care essential oils.

[0076] S2, drying and crushing the Ganoderma lucidum fruiting bodies, extracting them by hot water extraction, separating the solid residue from the obtained extract by centrifugation, adding ethanol to precipitate the polysaccharide, collecting the precipitate and removing small molecular impurities through a dialysis bag, freeze-drying the precipitate to prepare a powdered finished product, and obtaining a Ganoderma lucidum polysaccharide extract;

[0077] Furthermore, the Ganoderma lucidum fruiting body is dried and then crushed, and extracted by hot water extraction method, and the obtained extract is centrifuged to separate the solid residue, and the specific steps are as follows:

[0078] Place fresh Ganoderma lucidum fruiting bodies in a forced air drying oven and dry them to constant weight at 40°C, ensuring that the moisture content is less than 10%;

[0079] Use a high-speed grinder to grind the dried Ganoderma lucidum fruiting bodies into fine powder and pass through an 80-mesh sieve;

[0080] Add the crushed Ganoderma lucidum powder into deionized water at a ratio of 1:10;

[0081] Hot water extraction was performed at 85°C, and the duration was set to 2 hours;

[0082] After the extraction, the mixture was centrifuged at 3000 rpm for 15 minutes to separate the solid residue, and the supernatant was collected as the crude polysaccharide extract;

[0083] Add ethanol to precipitate the polysaccharide, collect the precipitate and remove small molecule impurities through a dialysis bag, freeze-dry and prepare a powdery finished product to obtain a Ganoderma lucidum polysaccharide extract. The specific steps are:

[0084] Slowly add 4 times the volume of 95% ethanol to the collected supernatant, stir evenly and let stand overnight to allow the polysaccharide to fully precipitate;

[0085] The precipitate was collected by centrifugation and washed several times with anhydrous ethanol to remove residual small molecule impurities;

[0086] The precipitate was placed in a dialysis bag and dialyzed with deionized water for 48 hours, with the dialysate replaced every 12 hours to completely remove small molecule impurities;

[0087] Finally, the dialyzed sample is freeze-dried to obtain a finished product of Ganoderma lucidum polysaccharide powder, which is Ganoderma lucidum polysaccharide extract.

[0088] It should be noted that the hot water extraction combined with ethanol precipitation and dialysis freeze-drying technology used in the extraction process of Ganoderma lucidum polysaccharides ensures the efficient extraction of polysaccharides from Ganoderma lucidum fruiting bodies while maximally removing small molecule impurities and improving the purity and stability of polysaccharides. This multi-step processing method not only effectively protects the structural integrity of polysaccharides, but also enhances their biological activity, so that the final Ganoderma lucidum polysaccharide powder product has excellent anti-aging properties, can significantly improve skin health, and provide key active ingredient support for skin care essential oils.

[0089] S3, using the obtained bayberry kernel flavonoids extract and ganoderma lucidum polysaccharide extract to prepare hollow liposomes by a thin film dispersion method, construct a microemulsion system, and form encapsulated nanoparticles;

[0090] Furthermore, the obtained bayberry kernel flavonoids extract and ganoderma lucidum polysaccharide extract were used to prepare hollow liposomes by thin film dispersion method, and the specific steps were as follows:

[0091] Dissolve the bayberry fruit core flavonoids extract and ganoderma lucidum polysaccharide extract in an appropriate amount of water;

[0092] Prepare lecithin as membrane material, dissolve lecithin in anhydrous ethanol to make a solution with a concentration of 10 mg / mL;

[0093] The ethanol was evaporated using a rotary evaporator at 40 °C under reduced pressure, so that the lipids were deposited on the container wall to form a uniform film;

[0094] adding an aqueous solution containing bayberry kernel flavonoids and ganoderma lucidum polysaccharide to the formed lipid film, and performing hydration treatment under gentle stirring to redisperse the lipid film to form hollow liposomes;

[0095] Ultrasonic treatment was used to refine the particle size of hollow liposomes, so that the average diameter of hollow liposomes was about 100 nm;

[0096] Construct a microemulsion system to form encapsulated nanoparticles. The specific steps are as follows:

[0097] The surfactant lecithin is used to reduce the interfacial tension and promote stable mixing between the oil and water phases;

[0098] The water phase and the oil phase were mixed under high-speed shear conditions and processed using a homogenizer at a speed of 10,000 rpm for 10 minutes to form a preliminary water-in-oil microemulsion;

[0099] The particle size distribution of the microemulsion was detected by dynamic light scattering method, and its average particle size was between 100-200 nm, forming encapsulated nanoparticles;

[0100] In the process of constructing the water-in-oil microemulsion system, the formula for optimizing the performance of the microemulsion system was introduced, as follows:

[0101] Optimize the performance of the microemulsion system and select the optimal liposome size and microemulsion stability. The expression is:

[0102]

[0103] Among them, S is the stability index, which indicates the long-term stability of the microemulsion system, L is the liposome particle size, V is the volume ratio, C is the concentration factor, D is the diffusion coefficient, and H is the interfacial tension.

[0104] It should be noted that.

[0105] S4, based on the microemulsion system, adding a synthetic penetration enhancer, adjusting the ratio of the constructed microemulsion system, determining the ratio of bayberry kernel flavonoids and ganoderma lucidum polysaccharide, preparing according to the determined ratio, and obtaining skin care essential oil;

[0106] Furthermore, based on the microemulsion system, a synthetic penetration enhancer is added, and the ratio of the constructed microemulsion system and the synthetic penetration enhancer combination is adjusted to determine the ratio of bayberry fruit core flavonoids and ganoderma lucidum polysaccharide. The specific steps are as follows:

[0107] Menthol derivatives were selected as synthetic penetration enhancers to open the stratum corneum gap;

[0108] Add menthol derivatives to the constructed microemulsion system according to the formula ratio and stir thoroughly;

[0109] Use a high-precision balance to accurately weigh the mass of each ingredient and mix them according to the formula ratio;

[0110] During the mixing process, the physical properties, viscosity and appearance of the system are continuously monitored to ensure uniform mixing and no stratification;

[0111] In the process of determining the ratio of bayberry kernel flavonoids to ganoderma lucidum polysaccharides, an optimization formula was introduced, and the expression is:

[0112]

[0113] Among them, O is the optimization index, which represents the optimal ratio of bayberry kernel flavonoids and Ganoderma lucidum polysaccharides, F is the flavonoid concentration, P is the polysaccharide concentration, A is the activity factor, B is the permeability coefficient, and C is the carrier effect.

[0114] It should be noted that adding a synthetic penetration enhancer and determining the optimal ratio of bayberry kernel flavonoids to ganoderma lucidum polysaccharides is one of the key steps of the present invention. Selecting a menthol derivative as a penetration enhancer can effectively open the gaps in the stratum corneum and enhance the penetration ability of large molecular weight active ingredients. At the same time, by introducing an innovative optimization formula to guide the selection of the optimal ratio, the synergistic effect between the two active ingredients is ensured to be optimal, thereby maximizing the comprehensive efficacy of the skin care essential oil. This method not only improves the safety and effectiveness of the product, but also provides users with a comfortable and efficient skin care solution, meeting the market demand for high-quality skin care products.

[0115] In summary, the supercritical carbon dioxide extraction method is used to remove impurities and extract bayberry kernel flavonoids, achieving an efficient and selective extraction process, which not only improves the extraction rate of flavonoid compounds, but also ensures that their biological activity is not damaged, providing high-quality basic materials for the formulation of subsequent skin care products. By drying, crushing, hot water extraction, centrifugal separation, ethanol precipitation, dialysis freeze drying and other steps of Ganoderma lucidum fruiting bodies, a high-purity Ganoderma lucidum polysaccharide extract is obtained. The final Ganoderma lucidum polysaccharide powder product has extremely high purity and stability, which enhances the overall anti-aging effect of skin care essential oils. The product not only has the function of protecting the skin from external influences, but also provides a guarantee for the safety and effectiveness of the product. By using the thin film dispersion method to prepare hollow liposomes from the obtained bayberry core flavonoids extract and ganoderma lucidum polysaccharide extract, and constructing a microemulsion system, the effective encapsulation of the active ingredients is achieved, which not only protects the active ingredients from the influence of the external environment, but also significantly improves their skin permeability and absorption efficiency, thereby enhancing the efficacy of skin care essential oils and providing a more lasting anti-aging effect. The active ingredients in bayberry core flavonoids and ganoderma lucidum polysaccharides can effectively inhibit the formation of acne, reduce the frequency of acne, and accelerate the healing of existing acne.

[0116] Example 2

[0117] Please refer to Table 1, which is the second embodiment of the present invention. In order to further verify the technical solution of the present invention, experimental simulation data of skin care essential oil based on bayberry kernel flavonoids for delaying cell aging and removing acne are given.

[0118] In order to verify the effect of the skin care essential oil based on bayberry fruit core flavonoids and ganoderma lucidum polysaccharide extract, a set of experiments was designed to compare the differences in skin permeability, antioxidant capacity and moisturizing effect between conventional skin care products in the prior art and the skin care essential oil of the present invention. Healthy adult female volunteers were selected as test subjects and the experiment was carried out in strict accordance with the protocol approved by the ethics committee.

[0119] First, the bayberry pits were cleaned to remove impurities and surface dirt, and then broken into small pieces. The treated bayberry pits were loaded into a supercritical carbon dioxide extraction device and extracted at a pressure of 30 MPa and a temperature of 40°C. The extract containing flavonoids was collected, and the highest extraction efficiency was ensured by optimizing the formula. Subsequently, a rotary evaporator was used to remove the solvent under reduced pressure, and the concentrate was retained. The target compound was purified and separated by silica gel column chromatography to finally obtain a high-purity bayberry pit flavonoid extract.

[0120] For the Ganoderma lucidum polysaccharide extract, fresh Ganoderma lucidum fruiting bodies are dried in a forced air drying oven at 40° C. to constant weight with a moisture content of less than 10%. The dried Ganoderma lucidum fruiting bodies are crushed into fine powder and added into deionized water in proportion, and extracted at 85° C. for 2 hours. After the extraction, the solid residue is separated by centrifugation at 3000 rpm for 15 minutes, and the supernatant is collected. 4 times the volume of 95% ethanol is slowly added to the collected supernatant, and the supernatant is allowed to stand overnight to precipitate the polysaccharide. The precipitate is collected by centrifugation and washed with anhydrous ethanol for multiple times, and finally dialyzed with deionized water in a dialysis bag for 48 hours, and freeze-dried to obtain the Ganoderma lucidum polysaccharide powder product.

[0121] Next, the bayberry core flavonoids extract and the ganoderma lucidum polysaccharide extract were dissolved in an appropriate amount of water, and lecithin was prepared as a membrane material and dissolved in anhydrous ethanol to prepare a solution with a concentration of 10 mg / mL. The ethanol was evaporated using a rotary evaporator at 40°C under reduced pressure to allow lipid deposition to form a uniform film. An aqueous solution containing active ingredients was added to the formed lipid film, and the solution was hydrated under gentle stirring to redisperse the lipid film to form hollow liposomes, and ultrasonic treatment was used to refine the particle size to an average diameter of about 100 nm.

[0122] When constructing the microemulsion system, lecithin was selected as a surfactant to reduce interfacial tension and promote stable mixing. The aqueous phase and the oil phase were mixed under high-speed shear conditions and processed using a homogenizer at a speed of 10,000 rpm for 10 minutes to form a preliminary microemulsion. The dynamic light scattering method was used to ensure that the average particle size was between 100-200 nm to form encapsulated nanoparticles.

[0123] In order to further improve the effect of skin care essential oils, menthol derivatives were selected as synthetic penetration enhancers and added to the constructed microemulsion system according to the formula ratio. The system was fully stirred and the mass of each ingredient was accurately weighed using a high-precision balance to ensure uniform mixing and no stratification. The optimal ratio was determined by introducing an optimization formula, thus achieving a highly effective anti-aging effect.

[0124] The table data is as follows:

[0125]

[0126] Table 1 Comparison of performance of different skin care products

[0127] Tabular data analysis:

[0128] As can be seen from Table 1, the skin care essential oil of the present invention is significantly superior to commercially available skin care products in a number of key parameters. First, in terms of antioxidant capacity, the skin care essential oil of the present invention reaches 220 μmol TE / g, which is much higher than 120 μmol TE / g of commercially available skin care product A and 150 μmol TE / g of commercially available skin care product B, indicating that the active ingredients in the skin care essential oil of the present invention have stronger free radical scavenging ability and can more effectively combat skin aging.

[0129] Secondly, skin permeability is one of the important indicators for measuring the effectiveness of skin care products. The skin permeability of the skin care essential oil of the present invention reaches 45%, while that of commercially available skin care product A is only 25%, and that of commercially available skin care product B is 30%. This means that the active ingredients in the skin care essential oil of the present invention can penetrate into the skin faster and deeper, and exert a better anti-aging effect.

[0130] Furthermore, moisturizing effect is also an important criterion for evaluating skin care products. The skin care essential oil of the present invention can form a long-lasting moisturizing film on the skin surface, which lasts for up to 8 hours. In comparison, the moisturizing effects of commercially available skin care products A and B are 4 hours and 5 hours, respectively. This result proves that the skin care essential oil of the present invention has excellent moisturizing properties and helps to improve the moisture content and elasticity of the skin.

[0131] In addition, the stability index reflects the long-term stability of the microemulsion system. The stability index of the skin care essential oil of the present invention is as high as 0.95, which is significantly better than 0.7 of commercially available skin care product A and 0.8 of commercially available skin care product B. This high level of stability ensures that the product can maintain good performance throughout its shelf life.

[0132] The skin care essential oil of the present invention performs well in removing acne. The effective ingredients such as bayberry kernel flavonoids and ganoderma lucidum polysaccharide can not only inhibit the formation of acne, but also accelerate the healing of existing acne. According to test data, the acne removal effect of the skin care essential oil of the present invention reaches 40%, which is significantly better than 10% of commercially available skin care product A and 15% of commercially available skin care product B.

[0133] It should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present invention may be modified or replaced by equivalents without departing from the spirit and scope of the technical solutions of the present invention, which should all be included in the scope of the claims of the present invention.

Claims

1. A skin care essential oil based on bayberry kernel flavonoids for delaying cell aging and removing acne, characterized in that: include: The skin care essential oil is made of 89% bayberry fruit core flavonoids extract, 5% ganoderma lucidum polysaccharide extract, 2% penetration enhancer, 0.5% hyaluronic acid, 3% lecithin, and 0.5% preservative phenoxyethanol; removing impurities from bayberry core flavonoids, extracting bayberry core flavonoids by supercritical carbon dioxide extraction to obtain bayberry core flavonoids extract, removing the solvent by rotary evaporator, and separating the target compound by column chromatography purification technology to obtain bayberry core flavonoids extract; The ganoderma lucidum fruiting body is dried and then crushed, extracted by hot water extraction, the obtained extract is centrifuged to separate the solid residue, ethanol is added to precipitate the polysaccharide, the precipitate is collected and small molecular impurities are removed by dialysis bag, and freeze-dried to prepare a powdered finished product to obtain a ganoderma lucidum polysaccharide extract; The obtained bayberry kernel flavonoids extract and ganoderma lucidum polysaccharide extract were used to prepare hollow liposomes by thin film dispersion method, construct a microemulsion system, and form encapsulated nanoparticles. Based on the microemulsion system, a synthetic penetration enhancer is added, the ratio of the constructed microemulsion system is adjusted, the ratio of bayberry kernel flavonoids and ganoderma lucidum polysaccharide is determined, and the skin care essential oil is prepared according to the determined ratio to obtain.

2. A skin care essential oil for delaying cell aging and removing acne based on bayberry kernel flavonoids according to claim 1, characterized in that: The method of removing impurities from bayberry core flavonoids and extracting bayberry core flavonoids by supercritical carbon dioxide extraction to obtain bayberry core flavonoids extract comprises the following specific steps: The dried bayberry pits are cleaned to remove impurities and surface dirt, and the cleaned bayberry pits are broken into small pieces; The crushed bayberry kernels were placed in a supercritical carbon dioxide extraction device, and the extraction parameters were set to 30 MPa and 40° C., and the extraction was performed to collect the extract containing flavonoid compounds; The extraction conditions were optimized during the extraction process, and the expression is: Wherein, E is the extraction efficiency, P is the system pressure, which indicates the pressure applied during the extraction process, T is the temperature, which indicates the temperature during the extraction process, D is the diffusion coefficient, which represents the diffusion rate of the target component in SC-CO2, C is the concentration factor, and H is the polarity index of the target component; The bayberry kernel flavonoids extract is obtained.

3. A skin care essential oil for delaying cell aging and removing acne based on bayberry kernel flavonoids according to claim 1, characterized in that: The solvent is removed by a rotary evaporator, and the target compound is separated by column chromatography purification technology to obtain the bayberry kernel flavonoid extract, and the specific steps are: The carbon dioxide solvent used in the extraction process is removed under reduced pressure using a rotary evaporator, and a concentrated solution containing flavonoids is retained; The concentrate was purified by silica gel column chromatography using ethyl acetate-petroleum ether as eluent; The eluate is collected and detected by thin layer chromatography to obtain a high-purity bayberry kernel flavonoid extract.

4. The skin care essential oil for delaying cell aging and removing acne based on bayberry kernel flavonoids according to claim 1, characterized in that: The ganoderma lucidum fruiting body is dried and then crushed, extracted by hot water extraction method, and the obtained extract is centrifuged to separate the solid residue, and the specific steps are: Place fresh Ganoderma lucidum fruiting bodies in a forced air drying oven and dry them to constant weight at 40°C, ensuring that the moisture content is less than 10%; Use a high-speed grinder to grind the dried Ganoderma lucidum fruiting bodies into fine powder and pass through an 80-mesh sieve; Add the crushed Ganoderma lucidum powder into deionized water at a ratio of 1:10; Hot water extraction was performed at 85°C, and the duration was set to 2 hours; After the extraction, the mixture was centrifuged at 3000 rpm for 15 minutes to separate the solid residue, and the supernatant was collected as the crude polysaccharide extract.

5. The skin care essential oil for delaying cell aging and removing acne based on bayberry kernel flavonoids according to claim 1, characterized in that: The steps of adding ethanol to precipitate the polysaccharide, collecting the precipitate and removing small molecule impurities through a dialysis bag, and freeze-drying the precipitate to prepare a powdery finished product to obtain the Ganoderma lucidum polysaccharide extract are as follows: Slowly add 4 times the volume of 95% ethanol to the collected supernatant, stir evenly and let stand overnight to allow the polysaccharide to fully precipitate; The precipitate was collected by centrifugation and washed several times with anhydrous ethanol to remove residual small molecule impurities; The precipitate was placed in a dialysis bag and dialyzed with deionized water for 48 hours, with the dialysate replaced every 12 hours to completely remove small molecule impurities; Finally, the dialyzed sample is freeze-dried to obtain a finished product of Ganoderma lucidum polysaccharide powder, which is Ganoderma lucidum polysaccharide extract.

6. The skin care essential oil for delaying cell aging and removing acne based on bayberry kernel flavonoids according to claim 1, characterized in that: The obtained bayberry core flavonoids extract and ganoderma lucidum polysaccharide extract are used to prepare hollow liposomes by thin film dispersion method, and the specific steps are as follows: Dissolve the bayberry fruit core flavonoids extract and ganoderma lucidum polysaccharide extract in an appropriate amount of water; Prepare lecithin as membrane material, dissolve lecithin in anhydrous ethanol to make a solution with a concentration of 10 mg / mL; The ethanol was evaporated using a rotary evaporator at 40 °C under reduced pressure, so that the lipids were deposited on the container wall to form a uniform film; adding an aqueous solution containing bayberry kernel flavonoids and ganoderma lucidum polysaccharide to the formed lipid film, and performing hydration treatment under gentle stirring to redisperse the lipid film to form hollow liposomes; Ultrasonic treatment was used to refine the particle size of hollow liposomes so that the average diameter of hollow liposomes was about 100 nm.

7. The skin care essential oil for delaying cell aging and removing acne based on bayberry kernel flavonoids according to claim 1, characterized in that: The microemulsion system is constructed to form encapsulated nanoparticles, and the specific steps are: The surfactant lecithin is used to reduce the interfacial tension and promote stable mixing between the oil and water phases; The water phase and the oil phase were mixed under high-speed shear conditions and processed using a homogenizer at a speed of 10,000 rpm for 10 minutes to form a preliminary water-in-oil microemulsion; The particle size distribution of the microemulsion is detected by dynamic light scattering method, so that the average particle size is between 100-200nm, forming encapsulated nanoparticles.

8. The skin care essential oil for delaying cell aging and removing acne based on bayberry kernel flavonoids according to claim 1, characterized in that: In the process of constructing the water-in-oil microemulsion system, a formula for optimizing the performance of the microemulsion system is introduced, which is as follows: Optimize the performance of the microemulsion system and select the optimal liposome size and microemulsion stability. The expression is: Among them, S is the stability index, which indicates the long-term stability of the microemulsion system, L is the liposome particle size, V is the volume ratio, C is the concentration factor, D is the diffusion coefficient, and H is the interfacial tension.

9. The skin care essential oil for delaying cell aging and removing acne based on bayberry kernel flavonoids according to claim 1, characterized in that: The method is based on the microemulsion system, wherein a synthetic penetration enhancer is added, the ratio of the constructed microemulsion system is adjusted, and the ratio of the bayberry kernel flavonoids to the ganoderma lucidum polysaccharide is determined. The specific steps are as follows: Menthol derivatives were selected as synthetic penetration enhancers to open the stratum corneum gap; Add menthol derivatives to the constructed microemulsion system according to the formula ratio and stir thoroughly; Use a high-precision balance to accurately weigh the mass of each ingredient and mix them according to the formula ratio; During the mixing process, the physical properties, viscosity and appearance of the system are continuously monitored to ensure uniform mixing and no stratification.

10. The skin care essential oil for delaying cell aging and removing acne based on bayberry kernel flavonoids according to claim 1, characterized in that: In the process of determining the ratio of bayberry kernel flavonoids to ganoderma lucidum polysaccharide, an optimization formula is introduced, and the expression is: Among them, O is the optimization index, which represents the optimal ratio of bayberry kernel flavonoids and Ganoderma lucidum polysaccharides, F is the flavonoid concentration, P is the polysaccharide concentration, A is the activity factor, B is the permeability coefficient, and C is the carrier effect.

Images