Food chemical degrading enzyme separation method

Abstract

The invention discloses a food chemical degrading enzyme separation method, and relates to the technical field of food chemical degrading enzyme separation, and the food chemical degrading enzyme separation method comprises the following steps: a1, putting a proper amount of plant fruit pulp and precooled acetone into a cup-shaped container, and stirring the interior of the cup-shaped container under the action of an arranged stirring device; through mutual cooperation of a motor I, a threaded rod, a transverse plate, a Z-shaped plate and a mounting plate, the effect of driving a rotating rod to move out of the cup-shaped container after stirring is finished in the process of separating the degrading enzyme can be achieved; and in the process of driving the rotating rod to move upwards, the detachable filtering device can be driven to move upwards under the action of the arranged mounting plate, so that the effect of filtering and separating sediments in the cup-shaped container in the degrading enzyme separation operation process is achieved.

Description

Technical Field

[0001] The invention relates to a method for separating a degradation enzyme, in particular to a method for separating a food chemical degradation enzyme. Background Art

[0002] Enzymes exist in all living organisms, are synthesized by biological cells, and participate in chemical reactions related to metabolism. collar domain Degradation enzymes are needed, and they can be separated and extracted from the inside of plant fruits.

[0003] At present, there are still some defects and deficiencies in the use of the degradation enzyme separation method. The specific areas that need to be improved are as follows:

[0004] 1. Most of the existing degradative enzyme separation methods require operators to manually stir during use. Long-term stirring not only makes the operators feel tired, but also makes it difficult to ensure the uniformity of stirring.

[0005] 2. The existing methods for separating the degradative enzymes are usually complicated to operate, thereby reducing the efficiency of separating the degradative enzymes. Summary of the invention

[0006] The purpose of the present invention is to provide a method for separating food chemical degradative enzymes, so as to solve the problem that the existing degradative enzyme separation methods proposed in the above background technology mostly require operators to manually stir the food during use. Long-term stirring not only makes the operators feel tired, but also the uniformity of stirring is difficult to ensure.

[0007] To achieve the above object, the present invention provides the following technical solution: a method for separating food chemical degradation enzymes, comprising the following steps:

[0008] a1. Put an appropriate amount of plant fruit pulp and pre-cooled acetone into the cup-shaped container, and then stir the interior of the cup-shaped container under the action of a stirring device. After the stirring is completed, the driving device and the stirring device cooperate with each other to drive the detachable filtering device to move upward, so as to filter and collect the sediment;

[0009] a2. Then, the cup-shaped container is cleaned, and the collected precipitate is placed in a vacuum drying device for drying, and crude enzyme powder is obtained. Then, the crude enzyme powder and an appropriate amount of pre-cooled citric acid buffer solution are placed in the cup-shaped container together, and the solution is stirred by the cooperation of a driving device and a stirring device. After the stirring is completed, the liquid in the cup-shaped container is filtered under the action of the provided driving device, stirring device and detachable filtering device;

[0010] a3. The liquid obtained in a3 is centrifuged through the centrifugal device body to obtain a supernatant, which is a crude enzyme solution. The cup-shaped container is then cleaned and the obtained crude enzyme solution is placed into the cup-shaped container. The crude enzyme solution is then subjected to gradient salting-out treatment with 30%-70% saturated ammonium sulfate to obtain the separated degradation enzyme.

[0011] As a preferred technical solution of the present invention, the lower end of the driving device is fixedly connected to a bottom plate, the left side of the driving device clamps a cup-shaped container, a stirring device is arranged on the left side of the driving device, the lower end of the stirring device is fixedly connected to the upper end of the bottom plate, the left side of the stirring device is fixedly connected to a detachable filtering device, the lower end of the detachable filtering device is overlapped on the inner bottom surface of the cup-shaped container, the lower end of the cup-shaped container is overlapped with the bottom plate with a placement groove opened inside the upper end, and a vacuum drying device and a centrifugal device body are arranged on the right side of the upper surface of the bottom plate.

[0012] As a preferred technical solution of the present invention, the driving device includes a motor 1, the lower end of the motor 1 is fixedly connected to the upper end of the base plate, the output shaft of the motor 1 is fixedly connected to the lower end of the threaded rod, the threaded rod is threadedly connected to a horizontal plate with a threaded hole inside, the horizontal plate is movably connected to the right side of the stirring device through a circular hole opened inside the left end of the horizontal plate, the upper end of the horizontal plate is fixedly connected to the right side of the stirring device, and the right end of the horizontal plate is fixedly connected to an L-shaped toothed plate.

[0013] As a preferred technical solution of the present invention, the L-shaped tooth plate is meshed with a gear for transmission, the gear is fixedly connected to a rotating shaft one, the right end of the rotating shaft one is fixedly connected to a rotating plate, the right end of the rotating plate is movably connected to two push-pull plates, the relatively distant ends of the two push-pull plates are movably connected to two fixed shafts respectively, the left ends of the two fixed shafts are respectively fixedly connected to the right ends of two sliding plates, the left ends of the two sliding plates are fixedly connected to clamps, and a cup-shaped container is clamped between the two clamps.

[0014] As a preferred technical solution of the present invention, the two sliding plates are movably connected to the two fixing rods through the two circular holes opened therein, the front and rear ends of the two fixing rods are respectively fixedly connected to the relatively close ends of the two fixing plates, the lower ends of the two fixing plates are fixedly connected to the upper end of the base plate, the front and rear ends of the two fixing rods are respectively sleeved with springs 1, the four springs are grouped in twos and their relatively close ends are respectively fixedly connected to the relatively far ends of the two sliding plates, the four springs are grouped in twos and their relatively far ends are respectively fixedly connected to the relatively close ends of the two fixing plates, the rotating shaft 1 is movably connected to an L-shaped plate 1 with a connecting hole opened therein on the lower side, the lower end of the L-shaped plate 1 is fixedly connected to the upper end of the base plate, and the L-shaped plate 1 is movably connected to the upper end of the threaded rod through the circular hole opened at its upper end.

[0015] As a preferred technical solution of the present invention, the stirring device includes motor 2, the lower end of which is fixedly connected to the upper end of the bottom plate, the output shaft of motor 2 is fixedly connected to the lower end of rotating shaft 2, and rotating shaft 2 is movably connected to a rotating sleeve with two guide grooves provided therein through two guide sliders arranged thereon, and the rotating sleeve is movably connected to a horizontal plate with a circular hole provided therein at the left end, and a sprocket 1 is fixedly connected to the upper side of the rotating sleeve, and the sprocket 1 is transmission-connected to sprocket 2 through a set chain, and the sprocket 2 is fixedly connected to the upper end of the rotating rod.

[0016] As a preferred technical solution of the present invention, the upper side of the rotating rod is movably connected to a mounting plate with a connecting hole provided inside, the lower end of the mounting plate is fixedly connected to the upper end of the detachable filtering device, the upper end of the mounting plate is fixedly connected to two Z-shaped plates, the lower ends of the two Z-shaped plates are fixedly connected to the upper end of the cross plate, the two Z-shaped plates are movably connected to two limiting rods through circular holes provided inside their lower ends, the lower ends of the two limiting rods are fixedly connected to the upper end of the bottom plate, a number of stirring rods are arranged on the rotating rod, the second rotating shaft is movably connected to a second L-shaped plate with a connecting hole provided inside the upper end, and the lower end of the second L-shaped plate is fixedly connected to the upper end of the bottom plate.

[0017] As a preferred technical solution of the present invention, the detachable filtering device includes a T-shaped plug rod, and two T-shaped plug rods are provided. The upper ends of the two T-shaped plug rods are fixedly connected to the lower end of the mounting plate, and the lower ends of the two T-shaped plug rods are respectively sleeved with the upper ends of the two T-shaped sleeve rods, and the lower ends of the two T-shaped sleeve rods are fixedly connected to the lower end of the filter mesh plate, and the lower end of the filter mesh plate is overlapped on the inner bottom surface of the cup-shaped container. The inside of the two T-shaped plug rods is provided with a square groove, and the upper and lower sides of the two square grooves are fixedly connected with cross bars.

[0018] As a preferred technical solution of the present invention, the four cross bars are grouped in pairs and movably connected to four vertical plates with circular holes provided inside the upper and lower ends, the four vertical plates are grouped in pairs and their relatively distant ends are fixedly connected with pressing columns, the four pressing columns are grouped in pairs and pass through two square grooves and are respectively clamped with T-shaped sleeve rods with two clamping holes provided inside the two upper sides, the four cross bars are sleeved with springs 2, the four springs 2 are grouped in pairs and their left and right ends are respectively fixedly connected to the relatively close ends of the corresponding two vertical plates.

[0019] Compared with the prior art, the present invention has the following beneficial effects:

[0020] 1. The present invention can achieve the effect of driving the rotating rod to move out of the cup-shaped container after the stirring is completed during the process of separating the degradative enzyme through the mutual cooperation among the motor 1, the threaded rod, the horizontal plate, the Z-shaped plate and the mounting plate. In the process of driving the rotating rod to move upward, the detachable filtering device can be driven to move upward under the action of the mounting plate, thereby achieving the effect of filtering and separating the sediment inside the cup-shaped container during the degradative enzyme separation operation. In the process of driving the rotating rod to move upward, the rotating shaft 1 can be driven to rotate under the action of the L-shaped toothed plate and the gear. Through the rotation of the rotating shaft 1, the two clamping plates can be driven to move away from each other under the mutual cooperation among the rotating plate, the push-pull plate, the fixed shaft and the sliding plate. At this time, the cup-shaped container can be conveniently taken out from between the two clamping plates for convenient cleaning.

[0021] 2. The present invention can drive the rotating rod to rotate through the cooperation between the motor 2, the rotating shaft 2, the rotating sleeve, the sprocket 1 and the sprocket 2. The rotation of the rotating rod can achieve the stirring process of the liquid inside the cup-shaped container during the degradation enzyme separation process under the action of the several stirring rods arranged on it, avoiding the situation that the operator needs to stir manually during the traditional degradation enzyme separation process, which not only reduces the labor of the operator, but also ensures the uniformity of the stirring process. The filter screen plate can be quickly removed from the device through the cooperation between the T-shaped plug rod, T-shaped sleeve rod, pressing column, vertical plate, cross bar and spring 2, and then the sediment on the filter screen plate can be conveniently collected and processed during the degradation enzyme separation process. At the same time, the filter screen plate can be cleaned in time after the degradation enzyme separation is completed. The degradation enzyme separation method adopted in this application can greatly improve the efficiency of degradation enzyme separation. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 It is a schematic diagram of the main stereoscopic structure of the present invention;

[0023] Figure 2 It is a left-side stereoscopic structural schematic diagram of the present invention;

[0024] Figure 3 It is a schematic diagram of the main cross-sectional three-dimensional structure of the present invention;

[0025] Figure 4 It is a schematic diagram of the main three-dimensional structure of the stirring device of the present invention;

[0026] Figure 5 It is a schematic diagram of the main three-dimensional structure of the driving device of the present invention;

[0027] Figure 6 It is a schematic diagram of the main three-dimensional structure of the detachable filtering device of the present invention;

[0028] Figure 7 It is a schematic diagram of the enlarged structure of point A of the present invention.

[0029] In the figure: 1 bottom plate, 2 cup-shaped container, 3 detachable filtering device, 31 filter screen plate, 32 T-shaped sleeve rod, 33 T-shaped plug rod, 34 cross bar, 35 pressing column, 36 square groove, 37 spring two, 38 vertical plate, 4 driving device, 41 fixed plate, 42 sliding plate, 43 motor one, 44 rotating plate, 45 fixed shaft, 46 push-pull plate, 47 gear, 48 rotating shaft one, 49 clamping plate, 410 spring one, 411 fixed rod, 412 threaded rod, 413 cross plate, 414 L-shaped tooth plate, 415 L-shaped plate one, 5 stirring device, 51 limiting rod, 52 motor two, 53 L-shaped plate two, 54 rotating shaft two, 55 rotating rod, 56 stirring rod, 57 Z-shaped plate, 58 rotating sleeve, 59 mounting plate, 510 sprocket two, 511 sprocket one, 6 centrifugal device body, 7 vacuum drying device. DETAILED DESCRIPTION

[0030] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0031] like Figure 1-7 As shown, the present invention provides a technical solution: a method for separating food chemical degradation enzymes, comprising the following steps:

[0032] a1. Put an appropriate amount of plant fruit pulp and pre-cooled acetone into the cup-shaped container 2, and then stir the interior of the cup-shaped container 2 under the action of the stirring device 5. After the stirring is completed, the detachable filtering device 3 is driven to move upward by the cooperation between the driving device 4 and the stirring device 5, so as to filter and collect the sediment;

[0033] a2. Then, the cup-shaped container 2 is cleaned, and the collected precipitate is placed in a vacuum drying device 7 for drying, and crude enzyme powder is obtained. Then, the crude enzyme powder and an appropriate amount of pre-cooled citric acid buffer solution are placed in the cup-shaped container 2, and stirred by the cooperation of the driving device 4 and the stirring device 5. After the stirring is completed, the driving device 4, the stirring device 5 and the detachable filtering device 3 are provided to achieve a filtering effect on the liquid inside the cup-shaped container 2;

[0034] a3. The liquid obtained in a3 is centrifuged through the centrifugal device body 6 to obtain a supernatant, which is a crude enzyme solution. Then, the cup-shaped container 2 is cleaned and the obtained crude enzyme solution is placed into the cup-shaped container 2. Then, it is subjected to gradient salting-out treatment with 30%-70% saturated ammonium sulfate to obtain the separated degradation enzyme.

[0035] The lower end of the driving device 4 is fixedly connected to the bottom plate 1, the left side of the driving device 4 holds the cup-shaped container 2, and the left side of the driving device 4 is provided with a stirring device 5, the lower end of the stirring device 5 is fixedly connected to the upper end of the bottom plate 1, and the left side of the stirring device 5 is fixedly connected to a detachable filtering device 3, the lower end of the detachable filtering device 3 overlaps the inner bottom surface of the cup-shaped container 2, the lower end of the cup-shaped container 2 overlaps the bottom plate 1 with a placement groove opened inside the upper end, and a vacuum drying device 7 and a centrifugal device body 6 are provided on the right side of the upper surface of the bottom plate 1, and the liquid inside the cup-shaped container 2 can be stirred by the stirring device 5. Automatic stirring is performed, thereby achieving the stirring process during the separation of the degradative enzyme, reducing the workload of the operators and ensuring the uniformity of the stirring process. The driving device 4 can drive the stirring device 5 to move in the up and down directions. In the process of driving the stirring device 5 to move in the up and down directions, the cup-shaped container 2 can be fixed and loosened, so that the device can effectively increase the stability of the cup-shaped container 2 during the separation of the degradative enzyme. The detachable filtering device 3 can have a filtering effect during the separation of the degradative enzyme.

[0036] The driving device 4 includes a motor 43, the lower end of which is fixedly connected to the upper end of the bottom plate 1, the output shaft of the motor 43 is fixedly connected to the lower end of the threaded rod 412, the threaded rod 412 is threadedly connected to a transverse plate 413 with a threaded hole inside, the transverse plate 413 is movably connected to the right side of the stirring device 5 through a circular hole inside its left end, the upper end of the transverse plate 413 is fixedly connected to the right side of the stirring device 5, and the right end of the transverse plate 413 is fixedly connected to an L-shaped toothed plate 414, the rotation of the output shaft of the motor 43 can drive the threaded rod 412 to rotate, and the rotation of the threaded rod 412 can drive the transverse plate 413 to move upward, so that the cup-shaped container 2 can be moved during the process of separating the degradation enzyme. After the internal liquid is stirred, the stirring device 5 and the detachable filtering device 3 are driven out from the inside of the cup-shaped container 2, the L-shaped tooth plate 414 is meshed with the gear 47 for transmission, the gear 47 is fixedly connected to the rotating shaft 1 48, the right end of the rotating shaft 1 48 is fixedly connected with a rotating plate 44, the right end of the rotating plate 44 is movably connected with two push-pull plates 46, the relatively far ends of the two push-pull plates 46 are movably connected with two fixed shafts 45, the left ends of the two fixed shafts 45 are respectively fixedly connected to the right ends of the two sliding plates 42, the left ends of the two sliding plates 42 are fixedly connected with clamping plates 49, the cup-shaped container 2 is clamped between the two clamping plates 49, and the two sliding plates 42 are respectively connected to the two fixed rods 4 through the two circular holes opened inside them. 11 is movably connected, the front and rear ends of the two fixing rods 411 are respectively fixedly connected to the relatively close ends of the two fixing plates 41, the lower ends of the two fixing plates 41 are fixedly connected to the upper end of the bottom plate 1, the front and rear ends of the two fixing rods 411 are sleeved with springs 410, the rotating shaft 48 is movably connected to an L-shaped plate 415 with a connecting hole opened inside the lower side, the lower end of the L-shaped plate 415 is fixedly connected to the upper end of the bottom plate 1, and the L-shaped plate 415 is movably connected to the upper end of the threaded rod 412 through the circular hole opened inside its upper end. In the process of driving the horizontal plate 413 to move upward, the rotating shaft 48 can be driven to rotate under the action of the L-shaped toothed plate 414 and the gear 47, and the rotating shaft 48 is driven to rotate. The rotation of the rotating plate 44, the push-pull plate 46 and the fixed shaft 45 can drive the two sliding plates 42 to move away from each other, and then drive the two clamping plates 49 to move away from each other. At this time, the cup-shaped container 2 can be easily taken out from between the two clamping plates 49, which is convenient for cleaning. The mutual cooperation between the fixed plate 41 and the fixed rod 411 can limit the movement direction of the sliding plate 42. The L-shaped plate 415 can support the rotating shaft 48 and the threaded rod 412. The spring 410 can drive the two sliding plates 42 to move away from each other, thereby ensuring the relative stability of the position between the two sliding plates 42.

[0037] The stirring device 5 includes a motor 2 52, the lower end of the motor 2 52 is fixedly connected to the upper end of the bottom plate 1, the output shaft of the motor 2 52 is fixedly connected to the lower end of the rotating shaft 2 54, the rotating shaft 2 54 is movably connected to a rotating sleeve 58 with two guide slides arranged thereon, the rotating sleeve 58 is movably connected to a horizontal plate 413 with a circular hole arranged therein at the left end, the upper side of the rotating sleeve 58 is fixedly connected to a sprocket 1 511, the sprocket 1 511 is transmission-connected to a sprocket 2 510 through a chain, and the sprocket 2 510 is fixedly connected to the upper end of the rotating rod 55 The upper side of the rotating rod 55 is movably connected to a mounting plate 59 with a connecting hole provided therein, and the rotation of the output shaft of the second motor 52 can drive the second rotating shaft 54 ​​to rotate. The rotation of the second rotating shaft 54 ​​can drive the rotating rod 55 to rotate under the action of the rotating sleeve 58, the sprocket one 511 and the sprocket two 510. The rotation of the rotating rod 55 can achieve the stirring process in the process of separating the degradation enzyme under the action of a plurality of stirring rods 56 provided thereon, which effectively reduces the tediousness of manual stirring by the operator and reduces the workload of the operator, and To ensure uniformity during the stirring process, the lower end of the mounting plate 59 is fixedly connected to the upper end of the detachable filtering device 3, and the upper end of the mounting plate 59 is fixedly connected to two Z-shaped plates 57, and the lower ends of the two Z-shaped plates 57 are fixedly connected to the upper end of the horizontal plate 413, and the two Z-shaped plates 57 are movably connected to the two limiting rods 51 through the circular holes opened inside the lower ends thereof, and the lower ends of the two limiting rods 51 are fixedly connected to the upper end of the bottom plate 1, and a plurality of stirring rods 56 are arranged on the rotating rod 55, and the rotating shaft 54 ​​is movably connected to the L-shaped plate 2 53 with a connecting hole opened inside the upper end, and the L-shaped The lower end of plate 2 53 is fixedly connected to the upper end of the bottom plate 1. In the process of driving the horizontal plate to move upward, the Z-shaped plate 57 can drive the mounting plate 59 to move upward accordingly, thereby achieving the process of driving the rotating rod 55 and the detachable filtering device 3 to move out of the cup-shaped container 2 after the liquid inside the cup-shaped container 2 is stirred. The movement direction of the mounting plate 59 can be limited by the mutual cooperation between the limiting rod 51 and the Z-shaped plate 57, and the L-shaped plate 2 53 can support the rotating shaft 2 54.

[0038] The detachable filtering device 3 includes a T-shaped plug rod 33, two T-shaped plug rods 33 are provided, the upper ends of the two T-shaped plug rods 33 are fixedly connected to the lower end of the mounting plate 59, the lower ends of the two T-shaped plug rods 33 are respectively sleeved with the upper ends of the two T-shaped sleeve rods 32, the lower ends of the two T-shaped sleeve rods 32 are fixedly connected to the lower end of the filter screen plate 31, and the lower end of the filter screen plate 31 is overlapped on the inner bottom surface of the cup-shaped container 2, the interiors of the two T-shaped plug rods 33 are provided with square grooves 36, the upper and lower sides of the two square grooves 36 are fixedly connected with cross bars 34, the four cross bars 34 are respectively connected in pairs with four vertical plates 38 with circular holes in the upper and lower ends, the four vertical plates 38 are respectively connected in pairs with pressing posts 35 at the relatively far ends, and the four pressing posts 35 are fixedly connected in pairs with pressing posts 35. The groups pass through two square grooves 36 respectively and are respectively engaged with two T-shaped sleeve rods 32 with two clamping holes provided inside the upper sides. The four cross bars 34 are sleeved with springs 37. The filter screen plate 31 is provided with a filtering effect in the process of separating the degradation enzyme. By pressing the two corresponding pressing clamping columns 35 to be close to each other, the four pressing clamping columns 35 are no longer engaged with the T-shaped sleeve rod 32 with two clamping holes provided inside the upper end. Then, by pulling the two T-shaped sleeve rods 32 downward, the upper ends of the two T-shaped sleeve rods 32 are separated from the lower ends of the two T-shaped plug rods 33 respectively. At this time, the filter screen plate 31 can be removed from the device, which is convenient for cleaning the filter screen plate 31 and collecting the filtered sediment on the filter screen plate 31.

[0039] The operating steps of the present invention are:

[0040] Put an appropriate amount of plant fruit pulp and pre-cooled acetone into the cup-shaped container 2, then start the motor 2 52 to drive the rotating shaft 2 54 to rotate, and the rotating shaft 54 ​​is driven to rotate under the action of the sprocket 1 511, the sprocket 2 510 and the rotating sleeve 58, and the rotating rod 55 is driven to rotate under the action of the set stirring rods 56 through the rotation of the rotating rod 55 to achieve the process of stirring the inside of the cup-shaped container 2, and after the stirring is completed, start the motor 1 43 to drive the threaded rod 412 to rotate, and the rotation of the threaded rod 412 can drive the horizontal plate 413 to move upward, and at this time, the rotating rod 55 and the detachable filter device 3 can be driven to move upward under the action of the set Z-shaped plate 57 and the mounting plate 59, and then the sediment inside the cup-shaped container 2 can be filtered and collected under the action of the set detachable filter device 3;

[0041] Next, the cup-shaped container 2 is cleaned, and the collected precipitate is placed in the vacuum drying device 7 for drying, and crude enzyme powder is obtained at this time. Then, the crude enzyme powder and an appropriate amount of pre-cooled citric acid buffer solution are placed in the cup-shaped container 2, and the above operation is repeated to stir the inside of the cup-shaped container 2. After the stirring is completed, the detachable filter device 3 is driven to move upward under the action of the provided driving device 4 and the stirring device 5, so as to achieve the filtering effect of the liquid inside the cup-shaped container 2;

[0042] The obtained liquid is centrifuged through the centrifugal device body 6 to obtain a supernatant, which is a crude enzyme solution. The cup-shaped container 2 is then cleaned and the obtained crude enzyme solution is placed in the cup-shaped container 2, and then subjected to a gradient salting-out treatment with 30%-70% saturated ammonium sulfate to obtain the separated degradation enzyme.

[0043] In the description of the present invention, it is necessary to understand that the indicated orientation or positional relationship is based on the orientation or positional relationship shown in the accompanying drawings, and is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present invention.

[0044] In the present invention, unless otherwise clearly stipulated and limited, for example, it can be a fixed connection, a detachable connection, or an integrated one; it can be a mechanical connection or an electrical connection; it can be directly connected or indirectly connected through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements. Unless otherwise clearly defined, for ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to the specific circumstances.

[0045] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A method for separating food chemical degradation enzymes, characterized in that: The following steps are involved: a1. Putting an appropriate amount of plant fruit pulp and pre-cooled acetone into the cup-shaped container (2), and then stirring the interior of the cup-shaped container (2) under the action of the stirring device (5). After the stirring is completed, the driving device (4) and the stirring device (5) cooperate with each other to drive the detachable filtering device (3) to move upward, thereby filtering and collecting the sediment; a2. The cup-shaped container (2) is then cleaned and the collected precipitate is placed in a vacuum drying device (7) for drying. At this time, crude enzyme powder is obtained. The crude enzyme powder and an appropriate amount of pre-cooled citric acid buffer solution are placed in the cup-shaped container (2) and stirred by the cooperation of the driving device (4) and the stirring device (5). After the stirring is completed, the liquid in the cup-shaped container (2) is filtered under the action of the provided driving device (4), the stirring device (5) and the detachable filtering device (3); a3. The liquid obtained in a3 is centrifuged through the centrifugal device body (6) to obtain a supernatant, i.e., a crude enzyme solution. The cup-shaped container (2) is then cleaned and the obtained crude enzyme solution is placed into the cup-shaped container (2). The crude enzyme solution is then subjected to a gradient salting-out treatment using 30%-70% saturated ammonium sulfate to obtain the separated degradation enzyme.

2. The device used in the method for separating chemical degradation enzymes of food according to claim 1, characterized in that: The lower end of the driving device (4) is fixedly connected to a bottom plate (1), the left side of the driving device (4) holds a cup-shaped container (2), the left side of the driving device (4) is provided with a stirring device (5), the lower end of the stirring device (5) is fixedly connected to the upper end of the bottom plate (1), the left side of the stirring device (5) is fixedly connected to a detachable filtering device (3), the lower end of the detachable filtering device (3) is overlapped on the inner bottom surface of the cup-shaped container (2), the lower end of the cup-shaped container (2) is overlapped with the bottom plate (1) with a placement groove formed inside the upper end, and a vacuum drying device (7) and a centrifugal device body (6) are provided on the right side of the upper surface of the bottom plate (1).

3. A food chemical degradation enzyme separation device according to claim 2, characterized in that: The driving device (4) comprises a motor 1 (43), the lower end of the motor 1 (43) is fixedly connected to the upper end of the bottom plate (1), the output shaft of the motor 1 (43) is fixedly connected to the lower end of a threaded rod (412), the threaded rod (412) is threadedly connected to a transverse plate (413) having a threaded hole therein, the transverse plate (413) is movably connected to the right side of the stirring device (5) via a circular hole therein at its left end, the upper end of the transverse plate (413) is fixedly connected to the right side of the stirring device (5), and the right end of the transverse plate (413) is fixedly connected to an L-shaped toothed plate (414).

4. A food chemical degradation enzyme separation device according to claim 3, characterized in that: The L-shaped toothed plate (414) is meshed with the gear (47) for transmission, the gear (47) is fixedly connected to the rotating shaft (48), the right end of the rotating shaft (48) is fixedly connected to a rotating plate (44), the right end of the rotating plate (44) is movably connected to two push-pull plates (46), the relatively distant ends of the two push-pull plates (46) are movably connected to two fixed shafts (45), the left ends of the two fixed shafts (45) are respectively fixedly connected to the right ends of the two sliding plates (42), the left ends of the two sliding plates (42) are fixedly connected to clamps (49), and the cup-shaped container (2) is clamped between the two clamps (49).

5. A food chemical degradation enzyme separation device according to claim 4, characterized in that: The two sliding plates (42) are movably connected to the two fixing rods (411) through two circular holes opened inside the two sliding plates (42), the front and rear ends of the two fixing rods (411) are respectively fixedly connected to the relatively close ends of the two fixing plates (41), the lower ends of the two fixing plates (41) are fixedly connected to the upper end of the bottom plate (1), the front and rear ends of the two fixing rods (41) are both sleeved with springs (410), the rotating shaft (48) is movably connected to an L-shaped plate (415) with a connecting hole opened inside the lower side, the lower end of the L-shaped plate (415) is fixedly connected to the upper end of the bottom plate (1), and the L-shaped plate (415) is movably connected to the upper end of the threaded rod (412) through the circular hole opened inside the upper end.

6. A food chemical degradation enzyme separation device according to claim 3, characterized in that: The stirring device (5) comprises a second motor (52), the lower end of which is fixedly connected to the upper end of the bottom plate (1), the output shaft of the second motor (52) is fixedly connected to the lower end of the second rotating shaft (54), the second rotating shaft (54) is movably connected to a rotating sleeve (58) having two guide slide grooves therein through two guide slide blocks arranged thereon, the rotating sleeve (58) is movably connected to a horizontal plate (413) having a circular hole therein at the left end, the upper side of the rotating sleeve (58) is fixedly connected to a sprocket wheel (511), the sprocket wheel (511) is transmission-connected to a second sprocket wheel (510) through a chain, and the second sprocket wheel (510) is fixedly connected to the upper end of the rotating rod (55).

7. A food chemical degradation enzyme separation device according to claim 6, characterized in that: The upper side of the rotating rod (55) is movably connected to a mounting plate (59) having a connecting hole therein, the lower end of the mounting plate (59) is fixedly connected to the upper end of the detachable filtering device (3), the upper end of the mounting plate (59) is fixedly connected to two Z-shaped plates (57), the lower ends of the two Z-shaped plates (57) are fixedly connected to the upper end of the transverse plate (413), the two Z-shaped plates (57) are movably connected to two limiting rods (51) through circular holes formed in the lower ends thereof, the lower ends of the two limiting rods (51) are fixedly connected to the upper end of the bottom plate (1), a plurality of stirring rods (56) are provided on the rotating rod (55), the second rotating shaft (54) is movably connected to a second L-shaped plate (53) having a connecting hole therein, the lower end of the second L-shaped plate (53) is fixedly connected to the upper end of the bottom plate (1).

8. A food chemical degradation enzyme separation device according to claim 7, characterized in that: The detachable filtering device (3) comprises a T-shaped plug rod (33), wherein two T-shaped plug rods (33) are provided, wherein the upper ends of the two T-shaped plug rods (33) are fixedly connected to the lower end of the mounting plate (59), the lower ends of the two T-shaped plug rods (33) are respectively sleeved with the upper ends of two T-shaped sleeve rods (32), the lower ends of the two T-shaped sleeve rods (32) are fixedly connected to the lower end of the filter screen plate (31), the lower end of the filter screen plate (31) is overlapped on the inner bottom surface of the cup-shaped container (2), the inside of the two T-shaped plug rods (33) are provided with square grooves (36), and the upper and lower sides of the inside of the two square grooves (36) are fixedly connected with cross bars (34).

9. A food chemical degradation enzyme separation device according to claim 8, characterized in that: The four cross bars (34) are movably connected to four vertical plates (38) with circular holes in the upper and lower ends in pairs, and the four vertical plates (38) are fixedly connected to the relatively far ends with pressing clamping columns (35) in pairs. The four pressing clamping columns (35) are respectively passed through two square grooves (36) in pairs and are clamped with two T-shaped sleeve rods (32) with two clamping holes in the upper sides. The four cross bars (34) are sleeved with springs (37).

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