Micro-particle skin transfer membrane preparation device and method based on temperature response effect

Abstract

The invention discloses a particle skin transfer membrane preparation device and method based on a temperature response effect, and the device comprises a case and a particle skin transfer membrane preparation mechanism installed in the case. The particle skin transfer film preparation mechanism mainly comprises a base, a rotating disc, a supporting frame, a supporting table, a container, a guide pipe, a lifting frame, a rotating support, a rotating motor, a stirring motor and a stirring rod. A refrigeration assembly is arranged in the base; a plurality of accommodating holes are formed in the turntable and are used for accommodating the forming vessels; the stepping motor drives the turntable to rotate, so that the forming vessels are aligned to outlets of the conduits one by one; the push rod piston is installed at the bottom of the rotating support and controlled to ascend and descend through a first lifting driving piece. A central processing module for controlling the stepping motor, the rotating motor, the stirring motor and the refrigerating assembly is arranged on the right side of the case; a transfer box is arranged below the machine box, and a drawer in the transfer box is used for storing the prepared particle skin transfer film. High-quality preparation of the particle skin transfer membrane is ensured by controlling actions of all the parts.

Description

Technical Field

[0001] The present invention relates to the technical field of microparticle skin transfer film preparation, and more specifically, to a microparticle skin transfer film preparation device and method based on temperature response effect. Background Art

[0002] In the field of burn treatment, patients with large-area burns (such as burns exceeding 50% of the body surface area) face the challenge of a serious shortage of autologous skin sources. The human body can provide very limited normal skin for transplantation, and traditional autologous skin transplantation methods, such as whole skin grafts or large-area skin grafts, obviously cannot meet the coverage needs of large-area burn wounds. For example, for a patient with a total body burn area of ​​80%, his unburned skin may only account for 20% of the body surface area. If conventional autologous skin transplantation is used, it is impossible to cover all wounds. This will not only prolong the treatment time, but also lead to a variety of serious complications, such as water and electrolyte disorders caused by a large amount of body fluid exudation, and infections caused by bacterial invasion of the wound. In severe cases, it can develop into sepsis and endanger the patient's life; long-term non-healing wounds will also lead to scar hyperplasia and contracture, seriously affecting the patient's limb function and appearance.

[0003] Although early skin transplantation techniques such as postage stamp skin transplantation have utilized limited autologous skin sources to a certain extent, there are still many shortcomings in practical applications. Postage stamp skin grafting technology attempts to achieve a wider coverage area with limited skin sources by dividing a small amount of autologous skin into smaller units and distributing them on the wound surface. However, the large gaps between postage stamp skin grafts prolong the wound healing time, and problems such as poor healing and infection are prone to occur during the healing process, resulting in poor overall treatment effect. In contrast, although large-sheet autologous skin transplantation technology can provide better healing effects, it is almost not feasible for patients with large-area burns due to the extremely high requirements for the amount of autologous skin sources. In addition, the complexity and diversity of large-area burn wounds also make large-sheet autologous skin transplantation technology face greater technical difficulties and risks in actual operation.

[0004] In recent years, with the in-depth study of the regeneration mechanism of skin tissue, scientists have gradually realized that the skin has a strong regenerative ability. Even if the skin is cut into tiny particles, as long as the right environment is provided, these micro-skins can still survive, proliferate and gradually cover the wound surface. This theory has laid a solid scientific foundation for the development of micro-skin transplantation technology. Micro-skin transplantation technology can cut limited autologous skin sources into tiny particles, greatly expanding the coverage area of ​​autologous skin. For example, after taking 1% of the body surface area of ​​autologous skin to prepare micro-skin, it can cover 10%-20% of the wound area, thereby greatly alleviating the problem of insufficient autologous skin sources. However, although micro-skin transplantation technology has significant advantages in theory, there are still a series of challenges in practical applications.

[0005] The primary problem is the uneven dispersion of micro-skins during the transplantation process. Due to the small size of the micro-skins, it is difficult to distribute them evenly on the wound surface, resulting in insufficient coverage in some areas, while other areas may be too dense, affecting the healing effect. Secondly, low transplantation efficiency is also a significant bottleneck. The process of preparing and transplanting micro-skins is complicated and takes a long time, and errors are prone to occur during the operation, further reducing the success rate of transplantation. Finally, the low survival rate of micro-skins after transplantation is also a problem that needs to be solved urgently. The micro-skins need to survive and begin to grow quickly on the wound surface after transplantation. If the survival rate of the micro-skins is lower than expected, it will not only prolong the wound healing time, but also increase the risk of infection and other complications. Summary of the invention

[0006] In order to overcome at least one technical problem existing in the above prior art, the present invention provides a device and method for preparing a microparticle skin transfer film based on a temperature response effect.

[0007] In order to solve the above technical problems, the technical solution of the present invention is as follows:

[0008] A device for preparing a microparticle skin transfer film based on a temperature response effect, the device comprising a chassis and a microparticle skin transfer film preparation mechanism installed in the chassis;

[0009] The microparticle skin transfer film preparation mechanism comprises a base fixedly mounted on the bottom of the chassis, and a refrigeration component is installed inside the base;

[0010] A turntable is installed on the top of the base, and the turntable is a hollow structure; a support frame is provided in the middle of the top of the base, and the support frame extends from the hollow part of the turntable;

[0011] A support platform is fixedly mounted on the support frame, a container is arranged on the support platform, and an outlet of the container is connected to an inlet of the conduit;

[0012] The turntable is provided with at least one receiving hole for receiving a forming dish; the turntable is configured to be driven by a stepping motor to rotate so as to drive the forming dishes received on the turntable to align with the outlet of the conduit one by one;

[0013] The support frame is also movably mounted with a first lifting assembly above the support platform; the first lifting assembly includes a lifting frame and a first lifting driving member for driving the lifting frame to move up and down along the support frame;

[0014] A rotating assembly is installed on the lifting frame, and the rotating assembly includes a rotating bracket and a rotating motor driving the rotating bracket to rotate;

[0015] A stirring assembly and a push rod piston are installed at the bottom of the rotating bracket. The rotating assembly is configured to cooperate with the first lifting assembly to move the stirring assembly or the push rod piston to a preset position, so that the stirring assembly or the push rod piston can stir or push the material in the container.

[0016] Furthermore, a container seat is fixedly mounted on the support platform, and the container seat is used to fix the container.

[0017] Further, the first lifting drive member includes a first motor, a first nut and a first screw rod, wherein:

[0018] The first screw rod is vertically arranged inside the support frame;

[0019] The first nut is mounted on the first screw rod, and the first nut is fixedly connected to the lifting frame;

[0020] The first motor is configured to drive the first lead screw to rotate;

[0021] The first nut is configured to move on the first screw rod as the first screw rod rotates, thereby driving the lifting frame to move up and down.

[0022] Furthermore, a catheter bracket is fixedly arranged on one side of the support platform close to the container, and the catheter bracket is used to fix the catheter.

[0023] Furthermore, the stirring assembly includes a stirring motor and a stirring rod, the stirring motor is installed at the bottom of the rotating bracket, and the stirring rod is installed at the driving end of the stirring motor.

[0024] Furthermore, a transfer box is arranged below the chassis, and the top of the transfer box is connected to the chassis; a pull-out drawer is arranged in the transfer box.

[0025] Furthermore, a transport channel is provided on the base, a second lifting assembly is provided at the transport channel, and the second lifting assembly includes a transfer platform and a first lifting driving member for driving the transfer platform to lift;

[0026] The accommodating holes on the turntable are through holes; when the turntable rotates, the accommodating holes on the turntable are aligned with the transport channel one by one, and the forming dishes in the accommodating holes fall on the transfer table when the accommodating holes are aligned with the transport channel;

[0027] The first lifting drive member is configured to drive the transfer platform to descend after the forming dish falls onto the transfer platform, thereby driving the forming dish to move into the drawer.

[0028] Furthermore, a fixed bracket is provided in the transfer box, and the second lifting assembly includes a second motor, a second nut and a second screw rod, wherein:

[0029] The second screw rod is vertically arranged on the fixing bracket;

[0030] The second nut is mounted on the second screw rod, and the second nut is fixedly connected to the transfer platform;

[0031] The second motor is configured to drive the second lead screw to rotate;

[0032] The second nut is configured to move on the second screw rod as the second screw rod rotates, thereby driving the transfer platform to rise and fall;

[0033] The drawer is provided with a placing table for placing the molding dish, and the placing table is provided with a receiving groove matching the transfer table, so that the molding dish can be placed flat on the placing table after the transfer table is embedded in the receiving groove.

[0034] Further, a laminar flow hood in communication with the chassis is provided on the top of the chassis;

[0035] The chassis is rotatably connected with a front cover, and the front cover is provided with an observation window;

[0036] The chassis is also equipped with a display screen, an adjustment knob and a button group, wherein the button group includes a power switch button and a start switch button;

[0037] The bottom of the chassis is provided with a foot for supporting the chassis;

[0038] A lighting lamp is installed inside the chassis;

[0039] The right side of the chassis is provided with a central processing module for controlling the stepping motor, rotating motor, stirring motor and refrigeration components, and the central processing module is connected to the display screen; the parameters on the display screen can be set by adjusting the knob to control the movement, rotation, stirring and other actions;

[0040] A power cord is connected to one side of the chassis, and the power cord is used to connect an external power source to provide power to the display screen, the refrigeration component, the stepper motor, the first lifting drive component, the rotating motor, the stirring component and the central processing module.

[0041] A method for preparing a microparticle skin transfer film based on a temperature response effect, using the aforementioned microparticle skin transfer film preparation device based on a temperature response effect, the method for preparing a microparticle skin transfer film based on a temperature response effect comprises the following steps:

[0042] Add gel compound liquid and micro-skin into the container, and place a forming dish at each receiving hole on the turntable;

[0043] The stirring assembly is aligned with the top opening of the container through the cooperation of the rotating assembly and the first lifting assembly, and the stirring assembly is driven to stir the gel composite liquid and the micro-skin in the container according to the stirring speed and stirring time set on the display screen, so as to evenly disperse the micro-skin in the gel composite liquid to obtain a micro-skin mixed liquid;

[0044] The push rod piston is aligned with the top opening of the container through the cooperation of the rotating assembly and the first lifting assembly, and after the push rod piston is aligned with the top opening of the container, the push rod piston is continuously driven by the first lifting assembly to push the micro-particle-skin mixed liquid in the container, and the micro-particle-skin mixed liquid in the container flows out from the outlet of the conduit due to the pushing of the push rod piston;

[0045] The turntable is driven to rotate by a stepping motor so that the forming dishes contained on the turntable are aligned with the outlets of the conduit one by one until each of the forming dishes is filled with a preset volume of the microparticle-skin mixture;

[0046] The micro-skin mixed liquid in each of the molding dishes is self-leveled under the action of its own gravity, and the micro-skin mixed liquid in each of the molding dishes is refrigerated by a refrigeration component installed inside the base, and the micro-skin mixed liquid in each of the molding dishes is refrigerated to obtain a micro-skin transfer film.

[0047] Compared with the prior art, the technical solution of the present invention has the following beneficial effects:

[0048] The present invention provides a device and method for preparing a microparticle skin transfer film based on a temperature response effect. By controlling the stirring speed, stirring time and cooling temperature, the uniform dispersion of the microparticle skin in the gel composite liquid and the high-quality preparation of the transfer film are ensured. The device is designed as a sealed structure, and the laminar flow hood provides a sterile operating environment, which effectively prevents contamination by bacteria and dust and improves safety. The fixed installation of the container seat and the catheter bracket ensures the stability and safety of the container and the catheter, and avoids accidental overflow or uneven transmission of the mixed liquid. The rotating assembly enables the stirring assembly and the push rod piston to be selectively aligned with the top opening of the container. The design of the pull-out drawer and the placement table not only facilitates the use and management of the transfer film, but also protects the integrity and safety of the transfer film. Compared with the traditional manual preparation method, the present invention is not only easier to operate, but also can significantly improve the preparation efficiency and quality, and improve the success rate of transplantation. BRIEF DESCRIPTION OF THE DRAWINGS

[0049] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0050] Figure 1 It is a schematic structural diagram of a microparticle skin transfer film preparation device based on temperature response effect provided in one embodiment of the present application;

[0051] Figure 2 It is a structural schematic diagram of a microparticle skin transfer film preparation mechanism in a microparticle skin transfer film preparation device based on a temperature response effect provided in one embodiment of the present application from a first viewing angle;

[0052] Figure 3 It is a structural schematic diagram of a microparticle skin transfer film preparation mechanism in a microparticle skin transfer film preparation device based on a temperature response effect provided in one embodiment of the present application from a second viewing angle;

[0053] Figure 4 It is a structural schematic diagram of a second lifting assembly in a microparticle skin transfer film preparation device based on a temperature response effect provided in one embodiment of the present application;

[0054] Figure 5 It is a schematic diagram of the outer side of a chassis of a microparticle skin transfer film preparation device based on a temperature response effect provided in one embodiment of the present application;

[0055] Description of the markings in the figure:

[0056] 1. Laminar flow hood; 2. Chassis; 3. Display screen; 4. Adjustment knob; 5. Button group; 6. Power cord; 7. Observation window; 8. Transfer box; 9. Front cover; 10. Machine feet;

[0057] 11. Turntable; 12. Base; 13. Lighting lamp;

[0058] 14. first screw rod; 15. lifting frame; 16. container seat; 17. support platform; 18. catheter bracket; 19. support frame; 20. accommodating hole; 21. forming dish;

[0059] 22. Rotating motor; 23. Rotating bracket; 24. Stirring motor; 25. Stirring rod; 26. Push rod piston; 27. Container; 28. Catheter;

[0060] 29. Second screw rod; 30. Fixed bracket; 31. Transfer table; 32. Drawer; 33. Placement table. DETAILED DESCRIPTION

[0061] In the following description, a large number of specific details are provided to provide a more thorough understanding of the present application. However, it is apparent to those skilled in the art that the present application can be implemented without one or more of these details. In other examples, in order to avoid confusion with the present application, some technical features well known in the art are not described.

[0062] It should be understood that the present application can be implemented in different forms and should not be construed as being limited to the embodiments presented herein. On the contrary, providing these embodiments will make the disclosure thorough and complete and fully convey the scope of the present application to those skilled in the art. In the accompanying drawings, the sizes and relative sizes of layers and regions may be exaggerated for clarity. The same reference numerals throughout represent the same elements.

[0063] In the description of the present invention, it should be understood that the terms "left side", "right side", "upper part", "lower part" and the like indicate positions or positional relationships based on the positions or positional relationships shown in the drawings, which are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operate in a specific orientation. The specific dimensions used in the embodiments are only for illustrating the technical solutions and do not limit the scope of protection of the present invention. It is understandable to those skilled in the art that some well-known structures and their descriptions in the drawings may be omitted.

[0064] Unless otherwise clearly specified and limited, the terms "install", "set", "connect", "fix" and the like should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements. For ordinary technicians in this field, the specific meanings of the above terms in this application can be understood according to specific circumstances.

[0065] In order to thoroughly understand the present application, a detailed structure will be presented in the following description to illustrate the technical solution proposed by the present application. The preferred embodiments of the present application are described in detail below, but in addition to these detailed descriptions, the present application may also have other implementation methods.

[0066] Embodiment 1:

[0067] like Figure 1-3 As shown, the present invention provides a technical solution:

[0068] A device for preparing a microparticle skin transfer film based on a temperature response effect, the device for preparing a microparticle skin transfer film based on a temperature response effect comprises a chassis 2 and a microparticle skin transfer film preparation mechanism installed in the chassis 2;

[0069] The microparticle skin transfer film preparation mechanism includes a base 12 fixedly mounted on the bottom of the chassis 2, and a refrigeration component is installed inside the base 12;

[0070] A turntable 11 is installed on the top of the base 12, and the turntable 11 is a hollow structure; a support frame 19 is provided in the middle of the top of the base 12, and the support frame 19 extends from the hollow part of the turntable 11;

[0071] A support platform 17 is fixedly mounted on the support frame 19, a container 27 is arranged on the support platform 17, and an outlet of the container 27 is connected to an inlet of a conduit 28;

[0072] The turntable 11 is provided with at least one receiving hole 20 for receiving the forming dish 21; the turntable 11 is configured to rotate by being driven by a stepping motor so as to drive the forming dishes 21 received on the turntable 11 to align with the outlet of the conduit 28 one by one; the turntable 11 rotates around the center of the base 12;

[0073] The support frame 19 is also movably mounted with a first lifting assembly above the support platform 17; the first lifting assembly includes a lifting frame 15 and a first lifting driving member for driving the lifting frame 15 to move up and down along the support frame 19;

[0074] The lifting frame 15 is provided with a rotating assembly, which includes a rotating bracket 23 and a rotating motor 22 for driving the rotating bracket 23 to rotate;

[0075] A stirring assembly and a push rod piston 26 are installed at the bottom of the rotating bracket 23. The rotating assembly is configured to cooperate with the first lifting assembly to move the stirring assembly or the push rod piston 26 to a preset position, so that the stirring assembly or the push rod piston 26 can stir or push the material in the container 27.

[0076] The microparticle skin transfer film preparation device based on the temperature response effect provided in this embodiment can automatically complete a series of functional operations to achieve the integration of the preparation process; at the same time, it can reduce the interference of human factors and improve the accuracy and efficiency of the operation.

[0077] Embodiment 2:

[0078] Based on Example 1, Figure 2 A container seat 16 is fixedly mounted on the support platform 17 , and the container seat 16 is used to fix the container 27 .

[0079] Further, refer to Figure 1 The number of the containing holes 20 is 6, and correspondingly, the number of the containers 27 is also 6, and the turntable 11 rotates in 60° steps.

[0080] Further, refer to Figure 2 , the first lifting drive member includes a first motor, a first nut and a first screw rod 14, wherein:

[0081] The first screw rod 14 is vertically arranged inside the support frame 19;

[0082] The first nut is mounted on the first screw rod 14, and the first nut is fixedly connected to the lifting frame 15;

[0083] The first motor is a stepping motor;

[0084] The first motor is configured to drive the first screw rod 14 to rotate;

[0085] The first nut is configured to move on the first screw rod 14 as the first screw rod 14 rotates, thereby driving the lifting frame 15 to move up and down.

[0086] Further, refer to Figure 2 A catheter bracket 18 is fixedly provided on one side of the support platform 17 close to the container 27 , and the catheter bracket 18 is used to fix the catheter 28 .

[0087] Further, refer to Figure 3 The stirring assembly includes a stirring motor 24 and a stirring rod 25 . The stirring motor 24 is installed at the bottom of the rotating bracket 23 , and the stirring rod 25 is installed at the driving end of the stirring motor 24 .

[0088] Further, refer to Figure 1 and Figure 4 A transfer box 8 is provided below the chassis 2, and the top of the transfer box 8 is connected to the chassis 2; a pull-out drawer 32 is provided in the transfer box 8.

[0089] Further, refer to Figure 4 A transport channel is provided on the base 12, and a second lifting assembly is provided at the transport channel. The second lifting assembly includes a transfer table 31 and a first lifting driving member that drives the transfer table 31 to rise and fall;

[0090] The receiving holes 20 provided on the turntable 11 are through holes; when the turntable 11 rotates, the receiving holes 20 on the turntable 11 are aligned with the transport channel one by one, and the forming dishes 21 in the receiving holes 20 fall on the transfer platform 31 when the receiving holes 20 are aligned with the transport channel;

[0091] The first lifting drive member is configured to drive the transfer platform 31 to descend after the forming dish 21 falls onto the transfer platform 31 , thereby driving the forming dish 21 to move into the drawer 32 .

[0092] Further, refer to Figure 4 The transfer box 8 is provided with a fixed bracket 30, and the second lifting assembly includes a second motor, a second nut and a second screw rod 29, wherein:

[0093] The second screw rod 29 is vertically arranged on the fixing bracket 30;

[0094] The second nut is mounted on the second screw rod 29, and the second nut is fixedly connected to the transfer platform 31;

[0095] The second motor is a stepping motor;

[0096] The second motor is configured to drive the second screw rod 29 to rotate;

[0097] The second nut is configured to move on the second screw rod 29 as the second screw rod 29 rotates, thereby driving the transfer platform 31 to rise and fall;

[0098] The drawer 32 is provided with a placement table 33 for placing the molding dish 21 , and the placement table 33 is provided with a receiving groove matching the transfer table 31 , so that the molding dish 21 can be placed flat on the placement table 33 after the transfer table 31 is embedded in the receiving groove.

[0099] Further, refer to Figure 5 The top of the chassis 2 is provided with a laminar flow hood 1 connected to the chassis 2, and air circulates between the laminar flow hood 1 and the inside of the chassis 2; the top of the equipment is equipped with a laminar flow hood, which can achieve the function of local sterility and control the number of colonies in the process of preparing the grain skin transfer film;

[0100] The chassis 2 is rotatably connected to a front cover 9, which can be opened and closed by flipping, and an observation window 7 is provided on the front cover 9;

[0101] The chassis 2 is also equipped with a display screen 3, an adjustment knob 4 and a button group 5. The display screen 3 is used to display the operating status and parameters of the device, and the button group 5 includes a power switch button and a start switch button. The specifications of the microparticle skin transfer film can be set by adjusting the device knob, and the molding dish 21 of the corresponding specifications can be selected to prepare microparticle skin transfer films of different sizes. This can meet the clinical needs of different wound sizes and shapes and improve the versatility and applicability of the equipment. The conventional microparticle skin transplantation operation process is too cumbersome and difficult to operate, and the entire process needs to be completed by the surgeon himself. When this device is introduced into the microparticle skin transplantation operation, it is only necessary to select the microparticle skin transfer film of the appropriate specifications according to the size of the wound, and then cut the prepared microparticle skin transfer film according to the shape of the wound and apply it to the wound, which greatly simplifies the process of microparticle skin transplantation and improves the efficiency of the operation.

[0102] The bottom of the chassis 2 is provided with a foot 10 for supporting the chassis 2;

[0103] A lighting lamp 13 is installed inside the chassis 2;

[0104] The right side of the chassis is provided with a central processing module for controlling the stepping motor, the rotating motor 22, the stirring motor and the refrigeration assembly, and the central processing module is connected to the display screen 3; the parameters on the display screen 3 can be set by adjusting the knob to control the movement, rotation, stirring and other actions;

[0105] A power cord 6 is connected to one side of the chassis 2, and the power cord 6 is used to connect an external power source to provide power to the display screen 3, the refrigeration component, the stepper motor, the first lifting drive component, the rotating motor 22 and the stirring component.

[0106] Furthermore, the size of the device for preparing the microparticle skin transfer film based on the temperature response effect is 0.8m*0.8m*0.6m, which is the size of a desktop device in a clinical operating room.

[0107] Furthermore, the base 12 is provided with a stepper motor and a refrigeration module for controlling the lifting frame 15, the turntable 11, and the transfer table 31; the controllers of the three stepper motors, the refrigeration assembly, the rotating motor 22, and the stirring motor 24 are all arranged on the right side of the chassis 2 and connected to the display screen 3. The power cord 6 is connected to the mains to power the entire device.

[0108] Specifically, the working principle of the microparticle skin transfer film preparation device based on temperature response effect is:

[0109] 1. Set the volume of gel composite liquid and the number of micro-skins on the equipment operation panel, place the container 27 on the container seat 16 on the platform, connect the lower end of the container 27 to the conduit 28, and fix the conduit 28 on the conduit bracket 18. At this time, the liquid outlet of the conduit 28 is aligned with the forming dish 21 in the turntable. Put the gel composite liquid and micro-skin into the container 27, put 6 forming dishes 21 on the turntable 11 at a time, and close the front cover 9.

[0110] 2. Press the power switch to power on the device. At this time, adjust the knob 4 to set the operating parameters such as the lifting speed and position of the lifting frame 15, the stirring rate and time of the stirring motor 24, the lifting speed and position of the transfer table 31, the turntable rotates 60°, that is, the time period of one work station, and the cooling temperature of the refrigeration component in the base.

[0111] 3. Start the equipment, the system automatically starts all processes, first rotate the bracket 23 to align one end of the stirring rod 25 with the container 27 filled with the micro-skin solution, stir the solution according to the set stirring speed and time, and after the stirring is completed, the micro-skin is completely and evenly dispersed. At this time, the rotating motor 22 aligns one end of the push rod piston 26 under the rotating bracket 23 with the container 27. The push rod piston 26 pushes the dispersed mixed liquid in the container 27 under the action of the lifting frame 15. The mixed liquid flows into the forming dish 21 of the turntable station through the outlet at the lower end of the container 27 through the conduit 28. The turntable 11 is horizontally calibrated to ensure that the forming dish 21 in the receiving hole 20 can always be horizontal. When the set volume of mixed micro-skin mixed liquid flows into the forming dish 21, the lifting frame 15 temporarily rotates the turntable 11 clockwise to the next forming dish 21 to align with the conduit port until the set volume of mixed liquid is filled in all 6 forming dishes 21. The mixed liquid in the forming dish 21 is self-leveled under the action of its own gravity. The entire turntable 11 is placed on the refrigeration component. Each forming dish 21 is guaranteed to have a refrigerated environment of 0-10°C to facilitate the formation of gel film sheets. After film formation, the system will record the start time and completion time of the film sheets in all the forming dishes and display them on the device display.

[0112] Fourth, the user takes the forming dish, and the system automatically determines that the earliest film is ready for removal. When the film-forming forming dish 21 is rotated to the transfer station via the turntable, it can just fall into the transfer platform 31 in the base. At this time, the transfer platform is transferred to the drawer 32 in the lower transfer box 8 under the action of the second screw 29. When the transfer is completed, the device prompts that the drawer 32 in the transfer box 8 can be pulled out to take out the forming dish 21 with the film, and the transfer platform 31 continues to rise to the transfer station to wait.

[0113] Embodiment 3:

[0114] The present invention provides a technical solution:

[0115] A method for preparing a microparticle skin transfer film based on a temperature response effect, using the aforementioned microparticle skin transfer film preparation device based on a temperature response effect, the method for preparing a microparticle skin transfer film based on a temperature response effect comprises the following steps:

[0116] Add gel composite liquid and micro-skin into the container 27, and place a molding dish 21 at each receiving hole 20 on the rotating disk 11;

[0117] The stirring assembly is aligned with the top opening of the container 27 by the cooperation of the rotating assembly and the first lifting assembly, and the stirring assembly is driven to stir the gel composite liquid and the micro-skin in the container 27 according to the stirring speed and stirring time set by the display screen 3, so that the micro-skin is evenly dispersed in the gel composite liquid to obtain a micro-skin mixed liquid;

[0118] The push rod piston 26 is aligned with the top opening of the container 27 through the cooperation of the rotating assembly and the first lifting assembly, and after the push rod piston 26 is aligned with the top opening of the container 27, the push rod piston 26 is continuously driven by the first lifting assembly to push the micro-skin mixed liquid in the container 27, and the micro-skin mixed liquid in the container 27 flows out from the outlet of the conduit 28 due to the pushing of the push rod piston 26;

[0119] The turntable 11 is driven to rotate by a stepping motor, so that the forming dishes 21 contained on the turntable 11 are aligned with the outlet of the conduit 28 one by one, until each of the forming dishes 21 is filled with a preset volume of the microparticle skin mixture;

[0120] The particle-skin mixture in each of the molding dishes 21 is self-leveled under the action of its own gravity. The particle-skin mixture in each of the molding dishes 21 is cooled by a refrigeration component installed inside the base 12. The ambient temperature is set between 0 and 10 degrees Celsius. The particle-skin mixture in each of the molding dishes 21 is refrigerated to obtain a particle-skin transfer film.

[0121] Furthermore, a temperature-responsive gel composite liquid is selected as a dispersion and film-forming base material; the gel composite liquid is added, and then micro-skins in an amount equal to the volume of the gel composite liquid are added; the equipment is started to disperse the micro-skins and stirred for a specified time.

[0122] Furthermore, a temperature-responsive gel composite liquid is selected and an equipment is used to evenly disperse the micro-skin inside it and then perform low-temperature molding. The low-temperature molded membrane has good mechanical properties, which facilitates the cutting and transfer of the micro-skin transfer membrane. The transferred membrane has good adhesion, and the micro-skin will adhere closely to the target wound surface, solving the problem of difficulty in fixing the micro-skin during the micro-skin transplantation operation, which affects the survival rate.

[0123] Furthermore, the speed of the dispersion stirring is 850rpm (±50rpm), and the temperature range of the low-temperature molding is 0-10°C (±1°C); the preparation method of the microparticle skin transfer film based on the temperature response effect is fully automated, and the automated process can prepare 6 microparticle skin transfer films at the same time.

[0124] The various devices selected in this application (components whose specific structures are not described) are all universal standard parts or components known to technical personnel in this field, and their structures and principles can be known to technical personnel through technical manuals or through conventional experimental methods.

[0125] Although example embodiments have been described herein with reference to the accompanying drawings, it should be understood that the above example embodiments are merely exemplary and are not intended to limit the scope of the present application to this. Those of ordinary skill in the art may make various changes and modifications therein without departing from the scope and spirit of the present application. All these changes and modifications are intended to be included within the scope of the present application as required by the appended claims.

[0126] Similarly, it should be understood that in order to streamline the present application and help understand one or more of the various application aspects, in the description of the exemplary embodiments of the present application, the various features of the present application are sometimes grouped together into a single embodiment, figure, or description thereof. However, the method of the present application should not be interpreted as reflecting the following intention: the claimed application requires more features than the features clearly stated in each claim. More specifically, as reflected in the corresponding claims, the application point is that the corresponding technical problem can be solved with less than all the features of a single disclosed embodiment. Therefore, the claims following the specific embodiment are hereby explicitly incorporated into the specific embodiment, wherein each claim itself serves as a separate embodiment of the present application.

[0127] In addition, those skilled in the art will appreciate that, although some embodiments described herein include certain features included in other embodiments but not other features, the combination of features of different embodiments is meant to be within the scope of the present application and form different embodiments. For example, in the claims, any one of the claimed embodiments can be used in any combination.

[0128] It should be noted that the above embodiments illustrate rather than limit the present application, and that those skilled in the art may devise alternative embodiments without departing from the scope of the appended claims. In the claims, any reference symbol between brackets should not be construed as a limitation to the claims. The use of the words first, second, and third, etc. does not indicate any order. These words may be interpreted as names.

Claims

1. A device for preparing microparticle skin transfer film based on temperature response effect, characterized in that: The device for preparing a microparticle skin transfer film based on a temperature response effect comprises a chassis (2) and a microparticle skin transfer film preparation mechanism installed in the chassis (2); The microparticle skin transfer film preparation mechanism comprises a base (12) fixedly mounted on the bottom of the chassis (2), and a refrigeration component is installed inside the base (12); A turntable (11) is installed on the top of the base (12), and the turntable (11) is a hollow structure; a support frame (19) is provided in the middle of the top of the base (12), and the support frame (19) extends from the hollow part of the turntable (11); A support platform (17) is fixedly mounted on the support frame (19), a container (27) is arranged on the support platform (17), and an outlet of the container (27) is connected to an inlet of a conduit (28); The turntable (11) is provided with at least one receiving hole (20) for receiving a forming dish (21); the turntable (11) is configured to rotate by being driven by a stepping motor so as to drive the forming dishes (21) received on the turntable (11) to align one by one with the outlet of the conduit (28); The support frame (19) is also movably provided with a first lifting assembly above the support platform (17); the first lifting assembly comprises a lifting frame (15) and a first lifting driving member for driving the lifting frame (15) to move up and down along the support frame (19); A rotating assembly is installed on the lifting frame (15), and the rotating assembly includes a rotating bracket (23) and a rotating motor (22) driving the rotating bracket (23) to rotate; A stirring assembly and a push rod piston (26) are installed at the bottom of the rotating bracket (23), and the rotating assembly is configured to cooperate with the first lifting assembly to move the stirring assembly or the push rod piston (26) to a preset position, so that the stirring assembly or the push rod piston (26) can stir or push the material in the container (27).

2. The device for preparing microparticle skin transfer film based on temperature response effect according to claim 1, characterized in that: A container seat (16) is fixedly mounted on the support platform (17), and the container seat (16) is used to fix the container (27).

3. The device for preparing microparticle skin transfer film based on temperature response effect according to claim 1, characterized in that: The first lifting drive member comprises a first motor, a first nut and a first screw rod (14), wherein: The first screw rod (14) is vertically arranged inside the support frame (19); The first nut is mounted on the first screw rod (14), and the first nut is fixedly connected to the lifting frame (15); The first motor is configured to drive the first screw (14) to rotate; The first nut is configured to move on the first screw rod (14) as the first screw rod (14) rotates, thereby driving the lifting frame (15) to move up and down.

4. The device for preparing microparticle skin transfer film based on temperature response effect according to claim 1, characterized in that: A catheter support (18) is fixedly arranged on one side of the support platform (17) close to the container (27), and the catheter support (18) is used to fix the catheter (28).

5. The device for preparing microparticle skin transfer film based on temperature response effect according to claim 1, characterized in that: The stirring assembly comprises a stirring motor (24) and a stirring rod (25), wherein the stirring motor (24) is mounted at the bottom of the rotating bracket (23), and the stirring rod (25) is mounted at the driving end of the stirring motor (24).

6. The device for preparing microparticle skin transfer film based on temperature response effect according to claim 1, characterized in that: A transfer box (8) is arranged below the chassis (2), and the top of the transfer box (8) is connected to the chassis (2); a drawer (32) that can be pulled out is arranged in the transfer box (8).

7. The device for preparing microparticle skin transfer film based on temperature response effect according to claim 6, characterized in that: A transport channel is provided on the base (12), a second lifting assembly is provided at the transport channel, and the second lifting assembly comprises a transfer platform (31) and a first lifting driving member for driving the transfer platform (31) to move up and down; The accommodating holes (20) provided on the turntable (11) are through holes; when the turntable (11) rotates, the accommodating holes (20) on the turntable (11) are aligned with the transport channel one by one, and the forming dishes (21) in the accommodating holes (20) fall onto the transfer platform (31) when the accommodating holes (20) are aligned with the transport channel; The first lifting drive member is configured to drive the transfer platform (31) to descend after the molding dish (21) falls onto the transfer platform (31), thereby driving the molding dish (21) to move into the drawer (32).

8. The device for preparing microparticle skin transfer film based on temperature response effect according to claim 7, characterized in that: The transfer box (8) is provided with a fixed bracket (30), and the second lifting assembly comprises a second motor, a second nut and a second screw rod (29), wherein: The second screw rod (29) is vertically arranged on the fixing bracket (30); The second nut is mounted on the second screw rod (29), and the second nut is fixedly connected to the transfer platform (31); The second motor is configured to drive the second screw rod (29) to rotate; The second nut is configured to move on the second screw rod (29) as the second screw rod (29) rotates, thereby driving the transfer platform (31) to rise and fall; The drawer (32) is provided with a placement table (33) for placing the molding dish (21), and the placement table (33) is provided with a receiving groove matching the transfer table (31), so that the molding dish (21) can be placed flat on the placement table (33) after the transfer table (31) is embedded in the receiving groove.

9. The device for preparing microparticle skin transfer film based on temperature response effect according to claim 1, characterized in that: A laminar flow hood (1) in communication with the chassis (2) is arranged on the top of the chassis (2); The chassis (2) is rotatably connected to a front cover (9), and the front cover (9) is provided with an observation window (7); The chassis (2) is also equipped with a display screen (3), an adjustment knob (4) and a button group (5), wherein the button group (5) includes a power switch button and a start switch button; The bottom of the chassis (2) is provided with a machine foot (10) for supporting the chassis (2); A lighting lamp (13) is installed inside the chassis (2); A power cord (6) is connected to one side of the chassis (2), and the power cord (6) is used to connect an external power source to provide power to the display screen (3), the refrigeration component, the stepper motor, the first lifting drive component, the rotating motor (22) and the stirring component.

10. A method for preparing a microparticle skin transfer film based on a temperature response effect, characterized in that: The microparticle skin transfer film preparation device based on the temperature response effect as claimed in any one of claims 1 to 9 is used, and the microparticle skin transfer film preparation method based on the temperature response effect comprises the following steps: Adding gel composite liquid and microparticle skin into a container (27), and placing a molding dish (21) at each receiving hole (20) on the rotating disk (11); The stirring assembly is aligned with the top opening of the container (27) by the cooperation of the rotating assembly and the first lifting assembly, and the stirring assembly is driven to stir the gel composite liquid and the micro-skin in the container (27) at a set stirring speed and stirring time, so that the micro-skin is evenly dispersed in the gel composite liquid to obtain a micro-skin mixed liquid; The push rod piston (26) is aligned with the top opening of the container (27) by the cooperation of the rotating assembly and the first lifting assembly, and after the push rod piston (26) is aligned with the top opening of the container (27), the push rod piston (26) is continuously driven by the first lifting assembly to push the micro-skin mixed liquid in the container (27), and the micro-skin mixed liquid in the container (27) flows out from the outlet of the conduit (28) due to the pushing of the push rod piston (26); The rotating disk (11) is driven to rotate by a stepping motor so that the forming dishes (21) contained on the rotating disk (11) are aligned with the outlet of the conduit (28) one by one until each of the forming dishes (21) is filled with a preset volume of the microparticle skin mixed liquid; The micro-skin mixed liquid in each of the molding dishes (21) is self-leveled under the action of its own gravity, and the micro-skin mixed liquid in each of the molding dishes (21) is cooled by a refrigeration component installed inside the base (12). The micro-skin mixed liquid in each of the molding dishes (21) is refrigerated to obtain a micro-skin transfer film.

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