Integrated demagnetized bead cell incubation equipment

Abstract

The invention discloses demagnetized bead cell incubation integrated equipment which comprises an equipment body, two independently arranged incubation chambers are arranged in the equipment body, a temperature adjusting component is arranged in each incubation chamber, a shaking table is arranged in each incubation chamber, the shaking table is provided with a positioning cavity for positioning incubation test tubes, and the incubation test tubes are arranged in the positioning cavity. A closing cover plate for closing the upper end opening of the incubation chamber is also arranged at the top of the equipment body; the equipment body is provided with a magnetic generator capable of generating a magnetic field at the bottom of each incubation chamber; according to the invention, a cell incubation function and a demagnetic bead function are integrated, so that during cell incubation work, transfer is not needed in multiple devices, time and labor are saved, through segmented control, the temperature rise rate and control precision of each stage are optimized, overshoot is reduced, the response speed is increased, and the target temperature is gradually approached; and temperature overshoot caused by one-time large-amplitude temperature rise is avoided.

Description

Technical Field

[0001] The present invention relates to the technical field related to cell incubation, and in particular to an integrated device for incubating cells with demagnetizable beads. Background Art

[0002] With the continuous progress of science and technology, human society has achieved leaps again and again. In the field of cell incubation technology, incubation beds are often used in order to better meet special requirements. Magnetic beads are often used to process samples in biochemical experiments, medical tests and other work. After the magnetic beads are put into the sample for proper treatment, the liquid and the magnetic beads need to be separated for subsequent operations. In order to separate the magnetic beads, the existing common practice is to place the test tube containing the magnetic beads in the test tube hole of the magnetic rack, and the magnet on the side or bottom of the test tube hole adsorbs the magnetic beads to the side wall or bottom of the test tube, and then uses the pipette tip to absorb the liquid, thereby separating the liquid and the magnetic beads. The existing cell incubation equipment is separated from the demagnetized beads, so that multiple devices are required for transfer during operation, which is time-consuming and labor-intensive. The temperature cannot be well controlled during use, and it has the characteristics of nonlinearity, time-varying and hysteresis. Summary of the invention

[0003] In order to solve the problem that the existing technology separates the liquid and the magnetic beads. The existing cell incubation equipment is separated from the demagnetizing beads, so that multiple devices are required for transfer during operation, which is time-consuming and labor-intensive. The temperature cannot be well controlled during use, and the nonlinearity, time-varying and hysteresis characteristics of the device are also problematic. The present invention provides a demagnetizing bead cell incubation integrated device.

[0004] In order to solve the above technical problems, the present invention provides the following technical solutions:

[0005] The present invention discloses an integrated device for incubating cells with demagnetizable beads, comprising a device body, wherein two independently arranged incubation chambers are arranged in the device body, and each incubation chamber is provided with a temperature regulating component, wherein a shaking table is arranged in the incubation chamber, and the shaking table is provided with a positioning cavity for positioning an incubation test tube, and a closing cover plate for closing an upper port of the incubation chamber is also arranged on the top of the device body;

[0006] The device body is provided with a magnetic force generator capable of generating a magnetic field at the bottom of each incubation chamber;

[0007] The temperature regulating component comprises a heating wire arranged on the inner wall of the incubation chamber, a temperature sensor for detecting the internal temperature of the incubation chamber is arranged in the incubation chamber, a control module is arranged on the equipment body, and a temperature detection mechanism for measuring the temperature of the heating wire is also arranged in the incubation chamber; the temperature sensor, the heating wire and the temperature detection mechanism are all connected to the control module;

[0008] The working method of the heating wire is to first set the target incubation temperature through the control module, set a reference temperature close to the target incubation temperature, and then divide the process of heating the heating wire to the reference temperature into several heating stages, and set a detection temperature for each stage to gradually approach the reference temperature, and the heating of the reference temperature to the target incubation temperature is recorded as the fine heating stage, and the difference between the reference temperature and the target incubation temperature is 0.5-1.0 degrees Celsius.

[0009] As a preferred technical solution of the present invention, the heating wire stays for n minutes in each heating stage.

[0010] As a preferred technical solution of the present invention, the shaker is fixed with temperature detection sample tubes at the four corners of the shaker, and the temperature detection sample tubes are provided with an incubation solution that is the same as the incubation test tubes, and the closed cover is also equipped with a liquid temperature sensor extending into the incubation solution, and the liquid temperature sensor is connected to the control module;

[0011] In each heating stage, when the temperature values ​​detected by the air temperature sensor and the liquid temperature sensor are close to each other, the heating wire enters the next heating stage until the reference temperature is reached.

[0012] As a preferred technical solution of the present invention, a heat insulation layer is provided on the inner wall of the closed cover.

[0013] As a preferred technical solution of the present invention, the closed cover is provided with mounting holes corresponding to the positions of the temperature detection sample tubes, and a mounting tube is threadedly installed in the mounting hole, and the liquid temperature sensor is installed on the inner mounting tube and inserted into the interior of the temperature detection sample tube.

[0014] As a preferred technical solution of the present invention, the device body is provided with a control switch for controlling the magnetic force generator and an adjusting switch for controlling the magnetic force of the magnetic generator.

[0015] As a preferred technical solution of the present invention, the control module of the fine heating stage adopts a PID control mode for the heating wire.

[0016] As a preferred technical solution of the present invention, the incubation tube does not contact the bottom of the incubation chamber.

[0017] The beneficial effects of the present invention are:

[0018] This integrated cell incubation device capable of demagnetizing beads integrates the cell incubation function and the demagnetizing bead function in one, so that during the cell incubation work, there is no need to transfer between multiple devices, which saves time and effort, and is provided with two independently arranged incubation chambers, and each incubation chamber is provided with a temperature regulating component, the incubation chamber is provided with a shaker, and the shaker is provided with a positioning cavity for positioning the incubation test tube, and the top of the device body is also provided with a closing cover for closing the upper port of the incubation chamber, so that the common incubation needs of different cells can be met, and the temperature can be adjusted by the set temperature regulating mechanism, wherein the first First, the target incubation temperature is set through the control module, and a reference temperature is set near the target incubation temperature. Then, the process of heating the heating wire to the reference temperature is divided into several heating stages, and a detection temperature is set for each stage to gradually approach the reference temperature. The reference temperature is heated to the target incubation temperature and is recorded as the fine heating stage. The difference between the reference temperature and the target incubation temperature is 0.5-1.0 degrees Celsius. Through segmented control, the temperature rise rate and control accuracy of each stage are optimized, overshoot is reduced, response speed is improved, and the target temperature is gradually approached to avoid temperature overshoot caused by a one-time large temperature increase. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] The accompanying drawings are used to provide further understanding of the present invention and constitute a part of the specification. They are used to explain the present invention together with the embodiments of the present invention and do not constitute a limitation of the present invention.

[0020] In the attached picture:

[0021] Figure 1 It is a schematic structural diagram of a demagnetizable bead cell incubation integrated device of the present invention;

[0022] Figure 2 This is a schematic diagram of the top ring structure of a demagnetizable bead cell incubation integrated device of the present invention;

[0023] Figure 3 The present invention is a device for demagnetizing beads and incubating cells in one device Figure 1 Schematic diagram of the structure at A in the middle;

[0024] Figure 4 The present invention is a schematic diagram of a limit stop block structure of a demagnetizable bead cell incubation integrated device.

[0025] In the figure: 1. Equipment body; 2. Incubation chamber; 3. Temperature regulating component; 4. Shaking table; 5. Positioning cavity; 6. Closing cover; 7. Magnetic generator; 8. Heating wire; 9. Temperature sensor; 10. Control module; 11. Temperature detection mechanism; 12. Temperature detection sample tube; 13. Liquid temperature sensor; 14. Mounting hole; 15. Mounting tube; 16. Control switch; 17. Adjustment switch. DETAILED DESCRIPTION

[0026] The preferred embodiments of the present invention are described below in conjunction with the accompanying drawings. It should be understood that the preferred embodiments described herein are only used to illustrate and explain the present invention, and are not used to limit the present invention.

[0027] Example: Figure 1 , Figure 2 , Figure 3 and Figure 4 As shown, the present invention is a demagnetizable bead cell incubation integrated device, comprising a device body 1, wherein two independently arranged incubation chambers 2 are arranged in the device body 1, and each incubation chamber 2 is provided with a temperature regulating component 3, wherein a shaking table 4 is arranged in the incubation chamber 2, and the shaking table 4 is provided with a positioning cavity 5 for positioning the incubation test tube, and the top of the device body 1 is also provided with a closing cover plate 6 for closing the upper port of the incubation chamber 2;

[0028] The device body is provided with a magnetic force generator 7 capable of generating a magnetic field at the bottom of each incubation chamber 2;

[0029] The temperature regulating member 3 includes a heating wire 8 arranged on the inner wall of the incubation chamber 2, a temperature sensor 9 for detecting the internal temperature of the incubation chamber 2 is arranged in the incubation chamber 2, a control module 10 is arranged on the device body 1, and a temperature detection mechanism 11 for measuring the temperature of the heating wire is also arranged in the incubation chamber 2; the temperature sensor 9, the heating wire 8 and the temperature detection mechanism 11 are all connected to the control module 10;

[0030] The working method of the heating wire 8 is to first set the target incubation temperature through the control module 10, set a reference temperature near the target incubation temperature, and then divide the process of heating the heating wire 8 to the reference temperature into several heating stages, and set a detection temperature for each stage to gradually approach the reference temperature, and the reference temperature is heated to the target incubation temperature as a fine heating stage, and the difference between the reference temperature and the target incubation temperature is 0.5-1.0 degrees Celsius. The cell incubation function and the demagnetization bead function are integrated into one, so that during the cell incubation work, there is no need to transfer between multiple devices, which saves time and effort, and by setting up two independently set incubation chambers, and each incubation chamber is provided with a temperature regulating component, the incubation chamber is provided with a shaker, and the shaker is provided with a positioning cavity for positioning the incubation test tube, and the top of the device body is also provided with a closing cover for closing the upper port of the incubation chamber, so that the common needs of incubation of different cells can be met. And the temperature is adjusted by the set temperature regulating mechanism, wherein firstly the control module is used To set the target incubation temperature, set a reference temperature near the target incubation temperature, and then divide the process of heating the heating wire to the reference temperature into several heating stages, and set a detection temperature for each stage to gradually approach the reference temperature, and the period from the reference temperature to the target incubation temperature is recorded as the fine heating stage. The difference between the reference temperature and the target incubation temperature is 0.5-1.0 degrees Celsius. Through segmented control, the temperature rise rate and control accuracy of each stage are optimized, overshoot is reduced, response speed is improved, and the target temperature is gradually approached to avoid temperature overshoot caused by a one-time large temperature increase.

[0031] The heating wire 8 stays for n minutes in each heating stage, so that the temperature is slowly increased, which can ensure that the culture is easily and evenly heated.

[0032] Among them, the shaker 4 is fixed with temperature detection sample tubes 12 at the four corners of the shaker 4, and the temperature detection sample tubes 12 are provided with an incubation solution same as that of the incubation test tubes, and the closed cover 6 is also equipped with a liquid temperature sensor 13 extending into the incubation solution, and the liquid temperature sensor 13 is connected to the control module 10; in this way, the heating temperature can be known more accurately, which facilitates temperature control.

[0033] In each of the heating stages, when the temperature values ​​detected by the liquid temperature sensor 13 and the temperature sensor of the temperature detection mechanism are close, the heating wire 8 enters the next heating stage until the reference temperature is reached. Through segmented control, the temperature rise rate and control accuracy of each stage are optimized, overshoot is reduced, response speed is improved, and the target temperature is gradually approached to avoid temperature overshoot caused by a one-time large-scale temperature increase.

[0034] Wherein, a heat insulating layer is provided on the inner wall of the closed cover plate 6, which plays a role in heat insulation.

[0035] Among them, the closing cover plate 6 is provided with a mounting hole 14 corresponding to the position of the temperature detection sample tube 12, and the mounting hole 14 is internally threaded with a mounting tube 15. The liquid temperature sensor 13 is installed on the inner mounting tube 15 and inserted into the interior of the temperature detection sample tube 12, so that it is convenient to install and disassemble the liquid temperature sensor (13).

[0036] The device body 1 is provided with a control switch 16 for controlling the magnetic force generator 7 and an adjustment switch 17 for controlling the magnetic force of the magnetic generator 7, so as to facilitate adjustment according to different usage requirements.

[0037] The control module of the fine heating stage adopts a PID control mode for the heating wire 8. The incubation test tube does not contact the bottom of the incubation chamber 2.

[0038] When working, this kind of integrated cell incubation device capable of demagnetizing beads integrates the cell incubation function and the demagnetizing bead function in one, so that during the cell incubation work, there is no need to transfer between multiple devices, which saves time and effort, and is provided with two independently arranged incubation chambers, and each incubation chamber is provided with a temperature regulating component, and the incubation chamber is provided with a shaker, and the shaker is provided with a positioning cavity for positioning the incubation test tube, and the top of the device body is also provided with a closing cover for closing the upper port of the incubation chamber, so that the common incubation needs of different cells can be met, and the temperature can be adjusted by the set temperature regulating mechanism, and its First, the target incubation temperature is set through the control module, and a reference temperature is set near the target incubation temperature. Then, the process of heating the heating wire to the reference temperature is divided into several heating stages, and a detection temperature is set for each stage to gradually approach the reference temperature. The reference temperature is heated to the target incubation temperature as the fine heating stage. The difference between the reference temperature and the target incubation temperature is 0.5-1.0 degrees Celsius. Through segmented control, the temperature rise rate and control accuracy of each stage are optimized, overshoot is reduced, response speed is improved, and the target temperature is gradually approached to avoid temperature overshoot caused by a one-time large temperature increase.

[0039] Finally, it should be noted that the above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or replace some of the technical features therein by equivalents. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A demagnetizable bead cell incubation integrated device, characterized in that: The device comprises a device body (1), wherein two independently arranged incubation chambers (2) are arranged in the device body (1), and each incubation chamber (2) is provided with a temperature regulating component (3), wherein a shaking table (4) is arranged in the incubation chamber (2), and wherein the shaking table (4) is provided with a positioning cavity (5) for positioning an incubation test tube, and a closing cover plate (6) for closing the upper port of the incubation chamber (2) is also arranged on the top of the device body (1); The device body is provided with a magnetic force generator (7) capable of generating a magnetic field at the bottom of each incubation chamber (2); The temperature regulating component (3) comprises a heating wire (8) arranged on the inner wall of the incubation chamber (2); an air temperature sensor (9) for detecting the internal temperature of the incubation chamber (2) is arranged in the incubation chamber (2); a control module (10) is arranged on the device body (1); and a temperature detection mechanism (11) for measuring the temperature of the heating wire is also arranged in the incubation chamber (2); the air temperature sensor (9), the heating wire (8) and the temperature detection mechanism (11) are all connected to the control module (10); The working method of the heating wire (8) is to first set the target incubation temperature through the control module (10), set a reference temperature close to the target incubation temperature, and then divide the process of heating the heating wire (8) to the reference temperature into a plurality of heating stages, and set a detection temperature for each stage to gradually approach the reference temperature, and the heating of the reference temperature to the target incubation temperature is recorded as a fine heating stage, and the difference between the reference temperature and the target incubation temperature is 0.5-1.0 degrees Celsius.

2. The demagnetizable bead cell incubation integrated device according to claim 1, characterized in that: The heating wire (8) stays in each heating stage for n minutes.

3. The demagnetizable bead cell incubation integrated device according to claim 1, characterized in that: The shaking table (4) is fixed with temperature detection sample tubes (12) at the four corners of the shaking table (4), and the temperature detection sample tubes (12) are provided with an incubation solution that is the same as that of the incubation test tubes, and the closing cover (6) is also equipped with a liquid temperature sensor (13) that extends into the incubation solution, and the liquid temperature sensor (13) is connected to the control module (10); In each heating stage, when the temperature values ​​detected by the air temperature sensor (9) and the liquid temperature sensor (13) are close to each other, the heating wire (8) enters the next heating stage until the reference temperature is reached.

4. The demagnetizable bead cell incubation integrated device according to claim 2, characterized in that: A heat insulation layer is provided on the inner wall of the closed cover plate (6).

5. The demagnetizable bead cell incubation integrated device according to claim 3, characterized in that: The closed cover plate (6) is provided with mounting holes (14) corresponding to the positions of the temperature detection sample tubes (12), and the mounting holes (14) are internally threaded with mounting tubes (15), and the liquid temperature sensor (13) is mounted on the internal mounting tube (15) and inserted into the interior of the temperature detection sample tube (12).

6. The demagnetizable bead cell incubation integrated device according to claim 5, characterized in that: The device body (1) is provided with a control switch (16) for controlling the magnetic force generator (7) and an adjustment switch (17) for controlling the magnetic force of the magnetic force generator (7).

7. The demagnetizable bead cell incubation integrated device according to claim 5, characterized in that: In the fine heating stage, the control module adopts a PID control mode for the heating wire (8).

8. The demagnetizable bead cell incubation integrated device according to claim 1, characterized in that: The incubation tube does not contact the bottom of the incubation chamber (2).

9. The demagnetizable bead cell incubation integrated device according to claim 1, characterized in that: The bottom of the incubation chamber (2) is provided with a supporting net for supporting the bottom of the incubation test tube.

Images