Sugarcane peptide powder and production process thereof

Abstract

The invention discloses sugarcane peptide powder and a production process thereof, and relates to the technical field of sugarcane peptide powder. The extraction rate of the sugarcane peptide is improved; comprising the following steps that S1, firstly, sugarcane leaves are selected, pretreatment is conducted through a cleaning and crushing mechanism, the cleanliness of the surfaces of the sugarcane leaves is guaranteed, the sugarcane leaves are in a good fresh degree, and the crushed sugarcane leaves are ground; s2, a fungal strain is selected to conduct fermentation treatment on the smashed sugarcane leaves, cellulose in the sugarcane leaves is degraded, the fermentation temperature ranges from 24 DEG C to 27 DEG C, the fermentation time ranges from 4 d to 5 d, NaCl with the concentration being 4 mol / L is added after fermentation, then fermentation is conducted for 4 d to 5 d, and then ultrafiltration is conducted to obtain solids. The sugarcane leaves needing to be processed are optimized and pretreated in the production process, so that the freshness and cleanliness of the raw materials are guaranteed, nutrient substances are provided in the fermentation process, and normal growth and metabolism of microorganisms in the fermentation process are promoted.

Description

Technical Field

[0001] The invention relates to the technical field of sugarcane peptide powder, in particular to sugarcane peptide powder and a production process thereof. Background Art

[0002] In the prior art, sugarcane peptide powder is an active peptide extracted from sugarcane leaves, which has the effects of regulating blood sugar, improving sleep quality and assisting weight loss. In the production process of sugarcane peptide powder, it is necessary to increase its extraction rate to reduce production costs. Based on this, the present application proposes a sugarcane peptide powder and a production process thereof;

[0003] After searching, the Chinese patent application number 202410234692.X discloses a preparation method and application of sugarcane peptide, and the preparation method includes the following steps: S1 pretreatment; S2 fermentation treatment: activate and culture the sugarcane leaf straw degrading fungus (Doratomyces p.) XW2020002Z strain and inoculate it into the crushed sugarcane leaves. After fermentation and culture for 2-3 days, add NaCl solution, stir and continue fermentation for 5-7 days and then sterilize; add water to the sterilized fermentation product, stir and remove salt by ultrafiltration to obtain a solid; S3 enzymatic hydrolysis: add Bacillus subtilis protease to the solid for enzymatic hydrolysis, and then add pepsin and pancreatic protease for enzymatic hydrolysis; centrifuge and separate by centrifuge to take the filtrate; S4 extraction.

[0004] The sugarcane peptide powder and its production process in the above patent have the following shortcomings: the environment provided by the fermentation and enzymatic hydrolysis process is not conducive to the microorganisms to react in the most suitable state, thus affecting the fermentation and enzymatic hydrolysis effects. Summary of the invention

[0005] The purpose of the present invention is to solve the shortcomings of the prior art and to propose a sugarcane peptide powder and a production process thereof.

[0006] In order to achieve the above object, the present invention adopts the following technical solutions:

[0007] A production process of sugarcane peptide powder comprises the following steps:

[0008] S1: First, the sugarcane leaves are selected and pre-processed by a cleaning and crushing mechanism to ensure the cleanliness and freshness of the sugarcane leaves, and the crushed sugarcane leaves are ground;

[0009] S2: Select fungal species to ferment the crushed sugarcane leaves to degrade the cellulose in the sugarcane leaves. The fermentation temperature is 24-27°C and the fermentation time is 4-5 days. After fermentation, NaCl with a concentration of 4 mol / L is added and fermented for another 4-5 days before ultrafiltration to obtain solid matter.

[0010] S3: adding enzymes to the fermented solids for enzymatic hydrolysis, wherein the type of enzymatic hydrolysis is protease, which decomposes the macromolecular protein in the sugarcane leaf solids into small molecular polypeptides, wherein the fermentation temperature of the protease is 25-40° C., and the pH of the fermentation environment is 8-9;

[0011] S4: extracting the solution by ultrasonic-assisted extraction and then purifying it by centrifugal separation;

[0012] S5: The extracted and purified sugarcane peptide solution is spray dried under vacuum, with an air inlet temperature of 140-160°C and an air outlet temperature of 60-70°C.

[0013] Preferably, in the process of selecting the sugarcane leaves in step S1, fresh sugarcane leaves picked within one day and free of insect pests are selected as the preparation raw materials, the sugarcane leaves are stored in a dry and cool environment, and the moisture content after drying is controlled to be 13-16%. The sugarcane leaves are ground in a ball mill during the grinding process, the grinding time is 60-65 minutes, and the ball-to-material ratio is 50:1 (g / g).

[0014] Further: the fermentation treatment in step S2 provides nutrients, including carbon sources and inorganic salts, during the fermentation process;

[0015] In the enzymatic hydrolysis treatment in step S3, ammonium bicarbonate is selected as a buffer system during the process of stabilizing the pH to provide an alkaline buffer environment for the enzymatic hydrolysis process;

[0016] In the process of using ultrasound to assist extraction in step S4, the time of ultrasound-assisted extraction is 60 minutes, and the power of ultrasound is 230w and the temperature is 62°C.

[0017] Further: the cleaning and crushing mechanism in the S step includes a processing box, a bracket is fixed to the top outer wall of the processing box by bolts, and a hydraulic cylinder is fixed to the top outer wall of the bracket by bolts, and a slide rail assembly is fixed to the output end of the hydraulic cylinder, and a slide rail assembly is slidably connected to the output end of the slide rail assembly, and a cross plate is welded at the bottom of the slide, and a clamping tooth is welded on the surface of the cross plate, a lifting rod is fixed to the bottom of the cross plate by screws, and a mounting block is slidably connected to the outer wall of the lifting rod, a cover plate is threadedly connected to the outer wall of the lifting rod, and a spring is clamped between the bottom of the mounting block and the top of the cover plate, one end of the mounting block is rotatably connected to a crushing roller, and a rectangular hole is opened on the circumferential outer wall of the crushing roller, a resistance plate is welded to the circumferential outer wall of the crushing roller, and a coil spring is clamped between the resistance plate and the mounting block, and one end of the crushing roller is connected to an air inlet assembly, and the circumferential outer wall of the crushing roller is keyed to a gear used in conjunction with the clamping tooth.

[0018] As a preferred solution of the present invention: the slide rail assembly includes a driving motor, a frame and a threaded rod, the frame is fixed to the output end of the hydraulic cylinder, and the threaded rod is rotatably connected between the inner walls on both sides of the frame, the slide plate is slidably connected in the slide groove of the top inner wall of the frame, and the slide plate and the threaded rod are threadedly connected, the outer wall on one side of the frame is fixed with a driving motor by bolts, and the output end of the driving motor and one end of the threaded rod are connected by a coupling.

[0019] As a further solution of the present invention: the air inlet assembly includes a slider and a lining rod, the lining rod is slidably connected to the inner wall of the slide groove opened on both sides of the processing box, and the slider and the lining rod are slidably connected, and a joint is installed on the outer wall of one side of the slider.

[0020] As a further solution of the present invention: a support plate is fixed between the inner walls on both sides of the processing box by bolts, a mounting frame is welded to the outer wall on the top of the processing box, and an electromagnet is fixed to the inner wall on the top of the mounting frame by screws, and clamping plates are welded to the inner walls on both sides of the mounting frame, and the through holes opened on the surface of the clamping plates are slidably connected with vertical rods, and an adsorption block is fixed to the top of the vertical rod by screws, and a spring is clamped between the adsorption block and the clamping plate, and a crushing tooth block is fixed to the bottom of the vertical rod by screws, and the crushing tooth block and the support plate are slidably connected.

[0021] On the basis of the above-mentioned solution: a telescopic rod is welded to the bottom of the frame, a guide rod is welded to the bottom of the telescopic rod, and a pressure plate is welded to the bottom of the guide rod.

[0022] On the basis of the above-mentioned solution: a liquid inlet valve and a liquid discharge valve are respectively fixed to the outer walls of both sides of the processing box by bolts.

[0023] A sugarcane peptide powder is produced by utilizing a production process of sugarcane peptide powder.

[0024] The beneficial effects of the present invention are:

[0025] 1. A sugarcane peptide powder and its production process, which ensures the freshness and cleanliness of the raw materials by optimizing and pretreating the sugarcane leaves to be processed during the production process.

[0026] 2. A sugarcane peptide powder and its production process, which provides nutrients during the fermentation process, promotes the normal growth and metabolism of microorganisms during the fermentation process, and improves the fermentation effect.

[0027] 3. A sugarcane peptide powder and its production process, in the process of stabilizing pH, ammonium bicarbonate is selected as the buffer system to provide an alkaline buffer environment for the enzymatic hydrolysis process, so that the protease maintains sufficient activity during the enzymatic hydrolysis process.

[0028] 4. A sugarcane peptide powder and a production process thereof. The cleaning and crushing mechanism in step S1 can be used to clean and break sugarcane leaves during pretreatment. The purpose of the breaking is to break the longer sugarcane leaves into shorter parts to facilitate subsequent ball milling treatment. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 It is a schematic diagram of the process of producing sugarcane peptide powder proposed by the present invention;

[0030] Figure 2 It is an overall schematic diagram of a cleaning and crushing mechanism of a production process of sugarcane peptide powder proposed by the present invention;

[0031] Figure 3 It is a side schematic diagram of a cleaning and crushing mechanism of a production process of sugarcane peptide powder proposed by the present invention;

[0032] Figure 4 It is a main schematic diagram of a cleaning and crushing mechanism of a production process of sugarcane peptide powder proposed by the present invention;

[0033] Figure 5 It is a partial schematic diagram of the main body of a cleaning and crushing mechanism of a production process of sugarcane peptide powder proposed by the present invention;

[0034] Figure 6 It is a schematic diagram of a support plate of a cleaning and crushing mechanism of a production process of sugarcane peptide powder proposed by the present invention;

[0035] Figure 7 It is a schematic diagram of a magnetic suction component of a cleaning and crushing mechanism of a production process of sugarcane peptide powder proposed by the present invention.

[0036] In the figure: processing box 1; liquid inlet valve 2; hydraulic cylinder 3; bracket 4; mounting frame 5; drive motor 6; drain valve 7; frame 8; threaded rod 9; guide rod 10; slider 11; joint 12; lining rod 13; pressure plate 14; telescopic rod 15; lifting rod 16; slide plate 17; latching tooth 18; cross plate 19; crushing roller 20; rectangular hole 21; gear 22; coil spring 23; cover plate 24; spring 25; mounting block 26; resistance plate 27; latching plate 28; adsorption block 29; electromagnet 30; support plate 31; crushing tooth block 32; vertical rod 33; spring 1 34. DETAILED DESCRIPTION

[0037] The technical solution of this patent is further described in detail below in conjunction with specific implementation methods.

[0038] The embodiments of the present invention are described in detail below, and examples of the embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present invention, and cannot be understood as limiting the present invention.

[0039] Embodiment 1:

[0040] A production process of sugarcane peptide powder, such as Figure 1 As shown, the following steps are included:

[0041] S1: First, the sugarcane leaves are selected and pre-processed by a cleaning and crushing mechanism to ensure the cleanliness and freshness of the sugarcane leaves, and the crushed sugarcane leaves are ground;

[0042] S2: Select fungal species to ferment the crushed sugarcane leaves to degrade the cellulose in the sugarcane leaves. The fermentation temperature is 24-27°C and the fermentation time is 4-5 days. After fermentation, NaCl with a concentration of 4 mol / L is added and fermented for another 4-5 days before ultrafiltration to obtain solid matter.

[0043] S3: adding enzymes to the fermented solids for enzymatic hydrolysis, wherein the type of enzymatic hydrolysis is protease, which decomposes the macromolecular protein in the sugarcane leaf solids into small molecular polypeptides, wherein the fermentation temperature of the protease is 25-40° C., and the pH of the fermentation environment is 8-9;

[0044] S4: extracting the solution by ultrasonic-assisted extraction and then purifying it by centrifugal separation;

[0045] S5: spray drying the extracted and purified sugarcane peptide solution under vacuum, with an air inlet temperature of 140-160°C and an air outlet temperature of 60-70°C;

[0046] In the process of selecting the sugarcane leaves in step S1, fresh sugarcane leaves picked within one day and free of insect pests are selected as the preparation raw materials, and the sugarcane leaves are stored in a dry and cool environment to avoid direct sunlight, and the moisture content after drying is controlled to be 13-16%;

[0047] The sugarcane leaves were ground in a ball mill for 60-65 min, with a ball-to-material ratio of 50:1 (g / g).

[0048] The fermentation treatment in step S2 provides nutrients, including carbon sources and inorganic salts, during the fermentation process;

[0049] In the enzymatic hydrolysis treatment in step S3, ammonium bicarbonate is selected as a buffer system during the process of stabilizing the pH to provide an alkaline buffer environment for the enzymatic hydrolysis process;

[0050] In the process of using ultrasound to assist extraction in step S4, the time of ultrasound-assisted extraction is 60 minutes, and the power of ultrasound is 230w and the temperature is 62°C.

[0051] Embodiment 2:

[0052] A production process of sugarcane peptide powder, such as Figure 1 As shown, the following steps are included:

[0053] S1: First, the sugarcane leaves are selected and pre-processed by a cleaning and crushing mechanism to ensure the cleanliness and freshness of the sugarcane leaves, and the crushed sugarcane leaves are ground;

[0054] S2: Select fungal species to ferment the crushed sugarcane leaves to degrade the cellulose in the sugarcane leaves. The fermentation temperature is 24-27°C and the fermentation time is 5-7 days. After fermentation, NaCl with a concentration of 4 mol / L is added and fermented for another 5-7 days before ultrafiltration to obtain solid matter.

[0055] S3: adding enzymes to the fermented solids for enzymatic hydrolysis, wherein the type of enzymatic hydrolysis is protease, which decomposes the macromolecular protein in the sugarcane leaf solids into small molecular polypeptides, wherein the fermentation temperature of the protease is 25-40° C., and the pH of the fermentation environment is 8-9;

[0056] S4: extracting the solution by ultrasonic-assisted extraction and then purifying it by centrifugal separation;

[0057] S5: spray drying the extracted and purified sugarcane peptide solution under vacuum, with an air inlet temperature of 140-160°C and an air outlet temperature of 60-70°C;

[0058] In the process of selecting the sugarcane leaves in step S1, fresh sugarcane leaves picked within one day and free of insect pests are selected as the preparation raw materials, and the sugarcane leaves are stored in a dry and cool environment to avoid direct sunlight, and the moisture content after drying is controlled to be 13-16%;

[0059] The sugarcane leaves were ground in a ball mill for 60-65 min, with a ball-to-material ratio of 50:1 (g / g).

[0060] The fermentation treatment in step S2 provides nutrients, including carbon sources and inorganic salts, during the fermentation process;

[0061] In the enzymatic hydrolysis treatment in step S3, ammonium bicarbonate is selected as a buffer system during the process of stabilizing the pH to provide an alkaline buffer environment for the enzymatic hydrolysis process;

[0062] In the process of using ultrasound to assist extraction in step S4, the time of ultrasound-assisted extraction is 60 minutes, and the power of ultrasound is 230w and the temperature is 62°C.

[0063] Embodiment 3:

[0064] A production process of sugarcane peptide powder, such as Figure 1 As shown, the following steps are included:

[0065] S1: First, the sugarcane leaves are selected and pre-processed by a cleaning and crushing mechanism to ensure the cleanliness and freshness of the sugarcane leaves, and the crushed sugarcane leaves are ground;

[0066] S2: Select fungal species to ferment the crushed sugarcane leaves to degrade the cellulose in the sugarcane leaves. The fermentation temperature is 24-27°C and the fermentation time is 4-5 days. After fermentation, NaCl with a concentration of 4 mol / L is added and fermented for another 4-5 days before ultrafiltration to obtain solid matter.

[0067] S3: adding enzymes to the fermented solids for enzymatic hydrolysis, wherein the type of enzymatic hydrolysis is protease, which decomposes the macromolecular protein in the sugarcane leaf solids into small molecular polypeptides, wherein the fermentation temperature of the protease is 25-40° C., and the pH of the fermentation environment is 8-9;

[0068] S4: extracting the solution by microwave-assisted extraction and then purifying it by centrifugal separation;

[0069] S5: spray drying the extracted and purified sugarcane peptide solution under vacuum, with an air inlet temperature of 140-160°C and an air outlet temperature of 60-70°C;

[0070] In the process of selecting the sugarcane leaves in step S1, fresh sugarcane leaves picked within one day and free of insect pests are selected as the preparation raw materials, and the sugarcane leaves are stored in a dry and cool environment to avoid direct sunlight, and the moisture content after drying is controlled to be 13-16%;

[0071] The sugarcane leaves were ground in a ball mill for 60-65 min, with a ball-to-material ratio of 50:1 (g / g).

[0072] The fermentation treatment in step S2 provides nutrients, including carbon sources and inorganic salts, during the fermentation process;

[0073] In the enzymatic hydrolysis treatment in step S3, ammonium bicarbonate is selected as a buffer system during the process of stabilizing the pH to provide an alkaline buffer environment for the enzymatic hydrolysis process;

[0074] In the process of using ultrasound to assist extraction in step S4, the time of microwave-assisted extraction is 60 minutes and the temperature is 62°C.

[0075] Embodiment 4:

[0076] A production process of sugarcane peptide powder, such as Figures 2 to 6 As shown, the cleaning and crushing mechanism in the step S1 includes a processing box 1, a support plate 31 is fixed between the inner walls on both sides of the processing box 1 by bolts, a bracket 4 is fixed to the outer wall on the top of the processing box 1 by bolts, and a hydraulic cylinder 3 is fixed to the outer wall on the top of the bracket 4 by bolts, and a slide rail assembly is fixed to the output end of the hydraulic cylinder 3, and a slide plate 17 is slidably connected to the output end of the slide rail assembly, and a cross plate 19 is welded to the bottom of the slide plate 17, and a clamping tooth 18 is welded on the surface of the cross plate 19, a lifting rod 16 is fixed to the bottom of the cross plate 19 by screws, and a mounting block 26 is slidably connected to the outer wall of the lifting rod 16, a cover plate 24 is connected to the outer wall of the bottom of the lifting rod 16 by threads, and a spring 25 is clamped between the bottom of the mounting block 26 and the top of the cover plate 24, one end of the mounting block 26 is rotatably connected to a crushing roller 20, and a rectangular hole 21 is opened on the circumferential outer wall of the crushing roller 20, and an air inlet assembly is connected to one end of the crushing roller 20, and a gear 22 used in conjunction with the clamping tooth 18 is connected to the circumferential outer wall of the crushing roller 20 by a key;

[0077] Before crushing the sugarcane leaves, the sugarcane leaves need to be cleaned and dried. The purpose of cleaning is to remove dust and other impurities attached to the surface of the sugarcane leaves. The sugarcane leaves are placed on the support plate 31. When the sugarcane leaves need to be cleaned, the crushing roller 20 is first driven downward by the hydraulic cylinder 3. When the crushing roller 20 moves to fit the surface of the sugarcane leaves, the crushing roller 20 is limited by the support plate 31. Then a certain amount of cleaning liquid is injected into the processing box 1. When the cleaning liquid covers the sugarcane leaves, the output end of the hydraulic cylinder 3 is driven to continue to move downward. During the downward movement, the slide rail assembly drives the lifting rod 16 to move downward along the mounting block 26. During this process, the spring 25 is gradually stretched. The cross plate 19 drives the latch 18 and the gear 22 to engage with each other during the synchronous downward movement, and the latch 18 is used to limit the gear 22.

[0078] When the gear 22 is limited, the crushing roller 20 cannot rotate along the mounting block 26. When the slide rail assembly drives the crushing roller 20 to move horizontally, the teeth on the outer wall of the crushing roller 20 are used to reciprocately rub the surface of the sugarcane leaves, and dust and other impurities on the surface of the sugarcane leaves are removed by rubbing, thereby achieving cleaning of the sugarcane leaves.

[0079] After the sugarcane leaves are cleaned, the output end of the hydraulic cylinder 3 moves in the opposite direction so that the latch 18 is disengaged from the gear 22. At this time, when the slide rail assembly drives the crushing roller 20 to move horizontally, the crushing roller 20 can roll along the surface of the sugarcane leaves. Since the rectangular holes 21 are distributed in strips on the outer wall of the crushing roller 20, when the air inlet assembly connected to the crushing roller 20 blows air toward the inside of the crushing roller 20, the hot air acts on the surface of the sugarcane leaves through the rectangular holes 21, thereby drying the cleaned sugarcane leaves. The crushing roller 20 dries the sugarcane leaves by rolling, so that the hot air can better contact the sugarcane leaves, thereby improving the drying rate.

[0080] The slide rail assembly includes a drive motor 6, a frame 8 and a threaded rod 9, wherein the frame 8 is fixed to the output end of the hydraulic cylinder 3, and the threaded rod 9 is rotatably connected between the inner walls on both sides of the frame 8, a slide plate 17 is slidably connected to a slide groove on the top inner wall of the frame 8, and the slide plate 17 and the threaded rod 9 are threadedly connected, a drive motor 6 is fixed to the outer wall of one side of the frame 8 by bolts, and the output end of the drive motor 6 and one end of the threaded rod 9 are connected by a coupling;

[0081] When the driving motor 6 drives the threaded rod 9 to rotate, the sliding groove opened on the top inner wall of the frame 8 is used to guide the moving trajectory and path of the slide plate 17, so that the slide plate 17 slides along the sliding groove under the drive of the threaded rod 9 to adjust the lateral position of the crushing roller 20.

[0082] The air inlet assembly includes a slider 11 and a lining rod 13, the lining rod 13 is slidably connected to the inner wall of the slide groove opened on both sides of the processing box 1, and the slider 11 and the lining rod 13 are slidably connected, and a joint 12 is installed on the outer wall of one side of the slider 11, and the joint 12 is rotatably connected to one end of the crushing roller 20;

[0083] When drying the sugarcane leaves, the joint 12 and the fan pipe are connected. The fan is preferably a hot air blower. The hot air generated by the hot air enters the crushing roller 20 through the joint 12 and is finally discharged through the rectangular hole 21 to dry the sugarcane leaves.

[0084] A contact plate 27 is welded to the outer circumferential wall of the crushing roller 20, and a coil spring 23 is clamped between the contact plate 27 and the mounting block 26;

[0085] In order to ensure that the latching teeth 18 and the gear 22 are accurately connected when they need to be engaged and to avoid the latching teeth 18 from getting stuck, a coil spring 23 is provided so that when the crushing roller 20 moves to the initial position, the crushing roller 20 is in a position where it can be engaged with the latching teeth 18, and the latching teeth 18 are smoothly engaged with the outer wall of the gear 22 after moving downward.

[0086] A mounting frame 5 is welded to the outer wall of the top of the processing box 1, and an electromagnet 30 is fixed to the inner wall of the top of the mounting frame 5 by screws, and a clamping plate 28 is welded to the inner walls on both sides of the mounting frame 5, and a through hole opened on the surface of the clamping plate 28 is slidably connected with a vertical rod 33, and an adsorption block 29 is fixed to the top of the vertical rod 33 by screws, and a spring 34 is clamped between the adsorption block 29 and the clamping plate 28, and a crushing tooth block 32 is fixed to the bottom of the vertical rod 33 by screws, and the crushing tooth block 32 and the support plate 31 are slidably connected;

[0087] When the sugarcane leaves are dried, in order to facilitate timely crushing of the sugarcane leaves, when the electromagnet 30 is energized, it generates attraction for the adsorption block 29, so that the crushing tooth block 32 slides up along the support plate 31 under the drive of the vertical rod 33, and the crushing tooth block 32 protrudes a certain distance relative to the support plate 31. The crushing tooth block 32 and the electromagnet 30 are used in cooperation with each other. When the crushing roller 20 rolls along the surface of the support plate 31, the sugarcane leaves are crushed by the crushing roller 20, and the crushing roller 20 and the crushing tooth block 32 are used to crush the sugarcane leaves under the action of pressure.

[0088] A telescopic rod 15 is welded to the bottom of the frame 8, a guide rod 10 is welded to the bottom of the telescopic rod 15, and a pressure plate 14 is welded to the bottom of the guide rod 10;

[0089] In order to prevent the sugarcane leaves from floating due to buoyancy during the cleaning process, the pressing plates 14 are used to press the two ends of the sugarcane leaves, thereby ensuring the stability of the sugarcane leaves during cleaning.

[0090] The outer walls of both sides of the treatment box 1 are respectively fixed with a liquid inlet valve 2 and a liquid outlet valve 7 by bolts;

[0091] The liquid level of the cleaning liquid in the processing box 1 can be adjusted by providing the liquid inlet valve 2 and the liquid outlet valve 7 .

[0092] Embodiment 5:

[0093] A production process of sugarcane peptide powder. In order to detect the extraction effect of the sugarcane peptide powder prepared in Examples 1-3, a comparative example is introduced in this example. The difference between the comparative example and Example 1 is that the comparative example is different from Example 1 in step S2. The difference is that the comparative example does not select fungal species to ferment sugarcane leaves, and other process parameters are consistent with Example 1. The extraction rate of sugarcane peptide after the test is shown in Table 1:

[0094] Embodiment 1 Embodiment 2 Embodiment 3 Comparative Example Extraction rate 4.9％ 51.％ 4.6％ 4.2％

[0095] Table 1

[0096] According to the extraction rate results in Table 1, it can be seen that in the process of fermentation of sugarcane leaves, appropriately increasing the fermentation time can increase the extraction rate of sugarcane peptides, and the fungal species have a more obvious effect on promoting the fermentation of sugarcane leaves. At the same time, the extraction effect of ultrasonic-assisted extraction is better than that of microwave-assisted extraction.

[0097] The above is a preferred specific implementation manner of the present invention, and the protection scope of the present invention is not limited thereto. Any modification, equivalent substitution and improvement made by any technician familiar with the field within the technical scope disclosed by the present invention in combination with the prior art or public common sense, within the spirit and principle of the present invention, shall be covered by the protection scope of the present invention.

Claims

1. A production process of sugarcane peptide powder, characterized in that: The following steps are involved: S1: First, the sugarcane leaves are selected and pre-processed by a cleaning and crushing mechanism to ensure the cleanliness and freshness of the sugarcane leaves, and the crushed sugarcane leaves are ground; S2: Select fungal species to ferment the crushed sugarcane leaves to degrade the cellulose in the sugarcane leaves. The fermentation temperature is 24-27°C and the fermentation time is 4-5 days. After fermentation, NaCl with a concentration of 4 mol / L is added and fermented for another 4-5 days before ultrafiltration to obtain solid matter. S3: adding enzymes to the fermented solids for enzymatic hydrolysis, wherein the type of enzymatic hydrolysis is protease, which decomposes the macromolecular protein in the sugarcane leaf solids into small molecular polypeptides, wherein the fermentation temperature of the protease is 25-40° C., and the pH of the fermentation environment is 8-9; S4: extracting the solution by ultrasonic-assisted extraction and then purifying it by centrifugal separation; S5: The extracted and purified sugarcane peptide solution is spray dried under vacuum, with an air inlet temperature of 140-160°C and an air outlet temperature of 60-70°C.

2. The production process of sugarcane peptide powder according to claim 1, characterized in that: In the process of selecting sugarcane leaves in step S1, fresh sugarcane leaves picked within one day and free of insect pests are selected as the preparation raw materials, and the sugarcane leaves are stored in a dry and cool environment. The moisture content after drying is controlled at 13-16%. The sugarcane leaves are ground in a ball mill during the grinding process. The grinding time is 60-65 minutes, and the ball-to-material ratio is 50:1 (g / g).

3. The production process of sugarcane peptide powder according to claim 2, characterized in that: The fermentation treatment in step S2 provides nutrients, including carbon sources and inorganic salts, during the fermentation process; In the enzymatic hydrolysis treatment in step S3, ammonium bicarbonate is selected as a buffer system during the process of stabilizing the pH to provide an alkaline buffer environment for the enzymatic hydrolysis process; In the process of using ultrasound to assist extraction in step S4, the time of ultrasound-assisted extraction is 60 minutes, and the power of ultrasound is 230w and the temperature is 62°C.

4. The production process of sugarcane peptide powder according to claim 3, characterized in that: The cleaning and crushing mechanism in step S1 comprises a processing box (1), a bracket (4) is fixed to the top outer wall of the processing box (1) by bolts, and a hydraulic cylinder (3) is fixed to the top outer wall of the bracket (4) by bolts, and a slide rail assembly is fixed to the output end of the hydraulic cylinder (3), and a slide plate (17) is slidably connected to the output end of the slide rail assembly, and a cross plate (19) is welded to the bottom of the slide plate (17), and a clamping tooth (18) is welded to the surface of the cross plate (19), a lifting rod (16) is fixed to the bottom of the cross plate (19) by screws, and the outer wall of the lifting rod (16) is slidably connected to a mounting block (26), and the lifting rod (16) The bottom outer wall is connected to a cover plate (24) by means of a thread, and a spring (25) is clamped between the bottom of the mounting block (26) and the top of the cover plate (24), one end of the mounting block (26) is rotatably connected to a crushing roller (20), and a rectangular hole (21) is provided on the circumferential outer wall of the crushing roller (20), a resistance plate (27) is welded to the circumferential outer wall of the crushing roller (20), and a coil spring (23) is clamped between the resistance plate (27) and the mounting block (26), one end of the crushing roller (20) is connected to an air inlet assembly, and the circumferential outer wall of the crushing roller (20) is key-connected to a gear (22) used in conjunction with the clamping tooth (18).

5. The production process of sugarcane peptide powder according to claim 4, characterized in that: The slide rail assembly comprises a drive motor (6), a frame (8) and a threaded rod (9); the frame (8) is fixed to the output end of the hydraulic cylinder (3), and the threaded rod (9) is rotatably connected between the inner walls on both sides of the frame (8); a slide plate (17) is slidably connected to a slide groove on the top inner wall of the frame (8), and the slide plate (17) and the threaded rod (9) are threadedly connected; a drive motor (6) is fixed to the outer wall of one side of the frame (8) by bolts, and the output end of the drive motor (6) and one end of the threaded rod (9) are connected by a coupling.

6. The production process of sugarcane peptide powder according to claim 5, characterized in that: The air inlet assembly comprises a slider (11) and a lining rod (13), wherein the lining rod (13) is slidably connected to the inner wall of a slide groove opened on both sides of the processing box (1), and the slider (11) and the lining rod (13) are slidably connected to each other, and a joint (12) is installed on the outer wall of one side of the slider (11).

7. The production process of sugarcane peptide powder according to claim 6, characterized in that: A support plate (31) is fixed between the inner walls on both sides of the processing box (1) by bolts, a mounting frame (5) is welded to the outer wall of the top of the processing box (1), and an electromagnet (30) is fixed to the inner wall of the top of the mounting frame (5) by screws, and a clamping plate (28) is welded to the inner walls on both sides of the mounting frame (5), and a through hole opened on the surface of the clamping plate (28) is slidably connected with a vertical rod (33), and an adsorption block (29) is fixed to the top of the vertical rod (33) by screws, and a spring (34) is clamped between the adsorption block (29) and the clamping plate (28), and a crushing tooth block (32) is fixed to the bottom of the vertical rod (33) by screws, and the crushing tooth block (32) and the support plate (31) are slidably connected.

8. The production process of sugarcane peptide powder according to claim 7, characterized in that: A telescopic rod (15) is welded to the bottom of the frame (8), a guide rod (10) is welded to the bottom of the telescopic rod (15), and a pressure plate (14) is welded to the bottom of the guide rod (10).

9. The production process of sugarcane peptide powder according to claim 8, characterized in that: The outer walls on both sides of the processing box (1) are respectively fixed with a liquid inlet valve (2) and a liquid outlet valve (7) by bolts.

10. A sugarcane peptide powder, characterized in that: The sugarcane peptide powder is produced using the production process of the sugarcane peptide powder described in any one of claims 1-9.

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