Collagen peptide capable of effectively delaying skin aging and preparation method thereof

Abstract

The invention relates to the technical field of collagen peptides, in particular to a collagen peptide capable of effectively delaying skin aging and a preparation method thereof.The preparation method comprises the steps that animal bones are cleaned, dried and smashed to obtain bone grains, the bone grains are degreased and decalcified, then organic acid and protease are added for enzymolysis, after enzymolysis is finished, enzyme deactivation is conducted, clear liquid is collected and filtered through a 3kDa ultrafiltration membrane, and the collagen peptide is obtained; the method comprises the following steps: collecting a filtrate, freezing and drying to obtain a collagen peptide crude product, adding the collagen peptide crude product into an ethanol aqueous solution to obtain a mixed solution, conveying the mixed solution into a reactor filled with an ionic liquid through a high-pressure constant-flow pump for deodorization, collecting the deodorized reaction liquid, filtering with a 3kDa ultrafiltration membrane, and performing spray drying. The collagen peptide finished product prepared by the method disclosed by the invention can be completely odorless, and mouse experiments show that the collagen peptide disclosed by the invention can delay skin aging by improving the activity of antioxidant enzyme and reducing the generation of free radicals.

Description

Technical Field

[0001] The present invention relates to the technical field of collagen peptides, in particular to a collagen peptide capable of effectively delaying skin aging and a preparation method thereof. Background Art

[0002] Collagen peptides are the hydrolysis products of collagen. Compared with collagen, the biologically active amino acids in the peptide molecules have obvious antioxidant effects, and have the effects of delaying skin aging, preventing atherosclerosis, and promoting bone cell growth.

[0003] Animal bones are rich in nutrients such as protein, minerals, and fat, among which collagen is the main component of bone-derived protein. Therefore, extracting collagen from animal bones and further preparing it into collagen peptides is a potential way to transform scientific and technological achievements. The comprehensive utilization of bone by-products can not only reduce environmental pollution, but also has high economic and social benefits. However, bone-derived collagen peptides will produce some unpleasant odors during the preparation and processing process, which seriously restricts their application and market promotion prospects. Summary of the invention

[0004] In view of the above technical problems, the present invention proposes a collagen peptide that can effectively delay skin aging and a preparation method thereof.

[0005] The technical solutions adopted are as follows:

[0006] A method for preparing collagen peptides that can effectively delay skin aging is as follows:

[0007] The animal bones are washed, dried and crushed to obtain bone particles. The bone particles are defatted and decalcified, and then organic acid and protease are added for enzymolysis. After the enzymolysis is completed, the enzyme is inactivated, the clear liquid is collected and filtered with a 3kDa ultrafiltration membrane, the filtrate is collected and freeze-dried to obtain a crude collagen peptide, the crude collagen peptide is added to an ethanol aqueous solution to obtain a mixed solution, the mixed solution is transported to a reactor filled with an ionic liquid by a high-pressure constant flow pump for deodorization, the deodorized reaction liquid is collected, filtered again with a 3kDa ultrafiltration membrane and spray-dried.

[0008] Further, the degreasing method is as follows:

[0009] Add the bone particles into a Soxhlet extractor, use petroleum ether as the extraction solvent, and reflux extraction for 1-5 hours.

[0010] Further, the decalcification method is as follows:

[0011] Add the defatted bone particles into the EDTA solution and continue to stir and decalcify for 16-40 hours. During this period, replace the fresh EDTA solution every 4-8 hours. After decalcification, collect the precipitate, wash it, and dry it.

[0012] Further, the organic acid is a combination of lauric acid, citric acid and glacial acetic acid;

[0013] The mass ratio of the lauric acid, citric acid and glacial acetic acid is 1-10:1-10:1-10.

[0014] Furthermore, the protease is any one or a combination of two or more of pepsin, alkaline protease, neutral protease, papain and trypsin.

[0015] Furthermore, the ionic liquid is supported on a molecular sieve.

[0016] Furthermore, the molecular sieve is SBA-15 molecular sieve.

[0017] Furthermore, the ionic liquid has a double acidic group.

[0018] Furthermore, the ionic liquid is 1-sulfonic acid butyl-3-methylimidazole hydrogen sulfate.

[0019] The present invention also provides a collagen peptide which can effectively delay skin aging, and is prepared by the above preparation method.

[0020] Beneficial effects of the present invention:

[0021] The invention provides a collagen peptide capable of effectively delaying skin aging and a preparation method thereof. Aiming at the problem that the peculiar smell of bone-derived collagen peptide affects the product quality in the prior art, an ionic liquid with a double acidic group is used as a high-activity catalyst to catalyze the reaction of impurities producing peculiar smell in the crude collagen peptide, such as fatty acids such as isobutyric acid, hexanoic acid and butyric acid, with ethanol to generate low-boiling-point esters, which are then removed by spray drying to improve the quality of the collagen peptide. The SBA-15 molecular sieve can be used as a carrier to form a covalent bond with the ionic liquid to firmly fix it, thereby improving the stability and preventing it from being easily lost, and the ionic liquid has a high degree of dispersibility, thereby increasing the contact area between the reactant and the ionic liquid and improving the reaction rate. In addition, the SBA-15 molecular sieve also has a certain catalytic activity for the esterification reaction, and can cooperate with the ionic liquid to improve the reaction rate. Compared with activated carbon deodorization, the finished collagen peptide prepared by the method of the invention can be completely odorless. Moreover, it can be known from mouse experiments that the collagen peptide of the invention can delay skin aging by improving the activity of antioxidant enzymes and reducing the generation of free radicals. DETAILED DESCRIPTION

[0022] If the specific conditions are not specified in the examples, the experiments were carried out under conventional conditions or the conditions recommended by the manufacturer. If the manufacturer of the reagents or instruments is not specified, they are all conventional products that can be purchased commercially. The techniques not mentioned in the present invention are all referenced to the prior art. Unless otherwise specified, the following examples and comparative examples are parallel experiments, using the same processing steps and parameters.

[0023] Embodiment 1:

[0024] This embodiment provides a method for preparing collagen peptides that can effectively delay skin aging:

[0025] Fresh horse tibia was thoroughly washed with deionized water and then dried. After being crushed, it was passed through a 20-mesh sieve to obtain bone particles. The bone particles were added to a Soxhlet extractor, and petroleum ether was used as the extraction solvent. Reflux extraction was performed for 1-5 hours to obtain defatted bone particles. The defatted bone particles were added to an EDTA solution with a mass concentration of 5% at a material-liquid mass ratio of 1:10. The solution was continuously stirred for decalcification for 36 hours, during which fresh EDTA solution was replaced every 6 hours. After decalcification, the precipitate was collected and thoroughly washed with deionized water and dried to obtain defatted and decalcified bone particles. The defatted and decalcified bone particles were added to deionized water at a mass ratio of 1:10, and 5% and 1% of organic acid and pepsin by mass of the defatted and decalcified bone particles were added respectively. The organic acid consisted of lauric acid, citric acid and glacial acetic acid in a mass ratio of 1:4:10. The solution was not stirred at 4°C. After 24 hours of enzymatic hydrolysis with stirring, the enzyme was inactivated and centrifuged, the clear liquid was collected and filtered through a 3 kDa ultrafiltration membrane, the filtrate was collected and freeze-dried to obtain a crude collagen peptide, the crude collagen peptide was added to an ethanol aqueous solution with a mass concentration of 10% to obtain a 0.05 g / ml mixed solution, and the mixed solution was transported to a fixed bed reactor at a flow rate of 0.5 ml / min for deodorization by a high-pressure constant flow pump, the fixed bed reactor was filled with 1-sulfonic acid butyl-3-methylimidazole hydrogen sulfate / SBA-15 molecular sieve, the bed height was 5 cm, the deodorization temperature was 70°C, the reaction solution after deodorization was collected and filtered again through a 3 kDa ultrafiltration membrane and spray-dried at 140°C to obtain a finished collagen peptide, and the short peptide yield was 58.3% measured by the trichloroacetic acid soluble polypeptide method.

[0026] Wherein, the preparation method of 1-sulfonic acid butyl-3-methylimidazole hydrogen sulfate / SBA-15 molecular sieve is as follows:

[0027] Add SBA-15 molecular sieve into 10% ethanol aqueous solution at a material-liquid mass ratio of 1:10 and mix well. Then add 1-sulfonic acid butyl-3-methylimidazole hydrogen sulfate at a mass ratio of 5% of the SBA-15 molecular sieve, stir rapidly for 30 minutes, and heat to evaporate the water.

[0028] Embodiment 2:

[0029] This embodiment provides a method for preparing collagen peptides that can effectively delay skin aging:

[0030] Fresh horse tibia was thoroughly washed with deionized water and then dried. After being crushed, it was passed through a 20-mesh sieve to obtain bone particles. The bone particles were added to a Soxhlet extractor, and petroleum ether was used as the extraction solvent. Reflux extraction was performed for 1-5 hours to obtain defatted bone particles. The defatted bone particles were added to an EDTA solution with a mass concentration of 5% at a material-liquid mass ratio of 1:10. The solution was continuously stirred for decalcification for 36 hours, during which fresh EDTA solution was replaced every 6 hours. After decalcification, the precipitate was collected and thoroughly washed with deionized water and dried to obtain defatted and decalcified bone particles. The defatted and decalcified bone particles were added to deionized water at a mass ratio of 1:10, and 5% and 1% of organic acid and pepsin by mass of the defatted and decalcified bone particles were added respectively. The organic acid consisted of lauric acid, citric acid and glacial acetic acid in a mass ratio of 1:4:10. The solution was not stirred at 4°C. After 24 hours of enzymatic hydrolysis with stirring, the enzyme was inactivated and centrifuged, the clear liquid was collected and filtered through a 3 kDa ultrafiltration membrane, the filtrate was collected and freeze-dried to obtain a crude collagen peptide, the crude collagen peptide was added to an ethanol aqueous solution with a mass concentration of 10% to obtain a 0.05 g / ml mixed solution, and the mixed solution was transported to a fixed bed reactor at a flow rate of 0.5 ml / min for deodorization by a high-pressure constant flow pump, the fixed bed reactor was filled with 1-sulfonic acid butyl-3-methylimidazole hydrogen sulfate / SBA-15 molecular sieve, the bed height was 5 cm, the deodorization temperature was 40°C, the reaction solution after deodorization was collected and filtered again through a 3 kDa ultrafiltration membrane and spray-dried at 140°C to obtain a finished collagen peptide, and the short peptide yield was 50.2% measured by the trichloroacetic acid soluble polypeptide method.

[0031] Wherein, the preparation method of 1-sulfonic acid butyl-3-methylimidazole hydrogen sulfate / SBA-15 molecular sieve is the same as that in Example 1.

[0032] Embodiment 3:

[0033] This embodiment provides a method for preparing collagen peptides that can effectively delay skin aging:

[0034] Fresh horse tibia was thoroughly washed with deionized water and then dried. After being crushed, it was passed through a 20-mesh sieve to obtain bone particles. The bone particles were added to a Soxhlet extractor, and petroleum ether was used as the extraction solvent. Reflux extraction was performed for 1-5 hours to obtain defatted bone particles. The defatted bone particles were added to an EDTA solution with a mass concentration of 5% at a material-liquid mass ratio of 1:10. The solution was continuously stirred for decalcification for 36 hours, during which fresh EDTA solution was replaced every 6 hours. After decalcification, the precipitate was collected and thoroughly washed with deionized water and dried to obtain defatted and decalcified bone particles. The defatted and decalcified bone particles were added to deionized water at a mass ratio of 1:10, and 5% and 1% of organic acid and pepsin by mass of the defatted and decalcified bone particles were added respectively. The organic acid consisted of lauric acid, citric acid and glacial acetic acid in a mass ratio of 1:4:10. The solution was not stirred at 4°C. After 24 hours of enzymatic hydrolysis with stirring, the enzyme was inactivated and centrifuged, the clear liquid was collected and filtered through a 3 kDa ultrafiltration membrane, the filtrate was collected and freeze-dried to obtain a crude collagen peptide, the crude collagen peptide was added to an ethanol aqueous solution with a mass concentration of 10% to obtain a 0.05 g / ml mixed solution, and the mixed solution was transported to a fixed bed reactor at a flow rate of 0.5 ml / min for deodorization by a high-pressure constant flow pump, the fixed bed reactor was filled with 1-sulfonic acid butyl-3-methylimidazole hydrogen sulfate / SBA-15 molecular sieve, the bed height was 5 cm, the deodorization temperature was 50°C, the reaction solution after deodorization was collected and filtered again through a 3 kDa ultrafiltration membrane and spray-dried at 140°C to obtain a finished collagen peptide, and the short peptide yield was 53.5% measured by the trichloroacetic acid soluble polypeptide method.

[0035] Wherein, the preparation method of 1-sulfonic acid butyl-3-methylimidazole hydrogen sulfate / SBA-15 molecular sieve is the same as that in Example 1.

[0036] Embodiment 4:

[0037] This embodiment provides a method for preparing collagen peptides that can effectively delay skin aging:

[0038] Fresh horse tibia was thoroughly washed with deionized water and then dried. After being crushed, it was passed through a 20-mesh sieve to obtain bone particles. The bone particles were added to a Soxhlet extractor, and petroleum ether was used as the extraction solvent. Reflux extraction was performed for 1-5 hours to obtain defatted bone particles. The defatted bone particles were added to an EDTA solution with a mass concentration of 5% at a material-liquid mass ratio of 1:10. The solution was continuously stirred for decalcification for 36 hours, during which fresh EDTA solution was replaced every 6 hours. After decalcification, the precipitate was collected and thoroughly washed with deionized water and dried to obtain defatted and decalcified bone particles. The defatted and decalcified bone particles were added to deionized water at a mass ratio of 1:10, and 5% and 1% of organic acid and pepsin by mass of the defatted and decalcified bone particles were added respectively. The organic acid consisted of lauric acid, citric acid and glacial acetic acid in a mass ratio of 1:4:10. The solution was not stirred at 4°C. After 24 hours of enzymatic hydrolysis with stirring, the enzyme was inactivated and centrifuged, the clear liquid was collected and filtered through a 3 kDa ultrafiltration membrane, the filtrate was collected and freeze-dried to obtain a crude collagen peptide, the crude collagen peptide was added to an ethanol aqueous solution with a mass concentration of 10% to obtain a 0.05 g / ml mixed solution, and the mixed solution was transported to a fixed bed reactor at a flow rate of 0.5 ml / min for deodorization by a high-pressure constant flow pump, the fixed bed reactor was filled with 1-sulfonic acid butyl-3-methylimidazole hydrogen sulfate / SBA-15 molecular sieve, the bed height was 5 cm, the deodorization temperature was 60°C, the reaction solution after deodorization was collected and filtered again through a 3 kDa ultrafiltration membrane and spray-dried at 140°C to obtain a finished collagen peptide, and the short peptide yield was 55.4% measured by the trichloroacetic acid soluble polypeptide method.

[0039] Wherein, the preparation method of 1-sulfonic acid butyl-3-methylimidazole hydrogen sulfate / SBA-15 molecular sieve is the same as that in Example 1.

[0040] Embodiment 5:

[0041] This embodiment provides a method for preparing collagen peptides that can effectively delay skin aging:

[0042] Fresh horse tibia was thoroughly washed with deionized water and then dried. After being crushed, it was passed through a 20-mesh sieve to obtain bone particles. The bone particles were added to a Soxhlet extractor, and petroleum ether was used as the extraction solvent. Reflux extraction was performed for 1-5 hours to obtain defatted bone particles. The defatted bone particles were added to an EDTA solution with a mass concentration of 5% at a material-liquid mass ratio of 1:10. The solution was continuously stirred for decalcification for 36 hours, during which fresh EDTA solution was replaced every 6 hours. After decalcification, the precipitate was collected and thoroughly washed with deionized water and dried to obtain defatted and decalcified bone particles. The defatted and decalcified bone particles were added to deionized water at a mass ratio of 1:10, and 5% and 1% of organic acid and pepsin by mass of the defatted and decalcified bone particles were added respectively. The organic acid consisted of lauric acid, citric acid and glacial acetic acid in a mass ratio of 1:4:10. The solution was not stirred at 4°C. After 24 hours of enzymatic hydrolysis with stirring, the enzyme was inactivated and centrifuged, the clear liquid was collected and filtered through a 3 kDa ultrafiltration membrane, the filtrate was collected and freeze-dried to obtain a crude collagen peptide, the crude collagen peptide was added to an ethanol aqueous solution with a mass concentration of 10% to obtain a 0.05 g / ml mixed solution, and the mixed solution was transported to a fixed bed reactor at a flow rate of 0.5 ml / min for deodorization by a high-pressure constant flow pump, the fixed bed reactor was filled with 1-sulfonic acid butyl-3-methylimidazole hydrogen sulfate / SBA-15 molecular sieve, the bed height was 5 cm, the deodorization temperature was 80°C, the reaction solution after deodorization was collected and filtered again through a 3 kDa ultrafiltration membrane and spray-dried at 140°C to obtain a finished collagen peptide, and the short peptide yield was 56.9% measured by the trichloroacetic acid soluble polypeptide method.

[0043] Wherein, the preparation method of 1-sulfonic acid butyl-3-methylimidazole hydrogen sulfate / SBA-15 molecular sieve is the same as that in Example 1.

[0044] Comparative Example 1:

[0045] The method is substantially the same as Example 1, except that lauric acid is not included in the organic acid.

[0046] This comparative example provides a method for preparing a collagen peptide that can effectively delay skin aging:

[0047] Fresh horse tibia was thoroughly washed with deionized water and then dried. After being crushed, it was passed through a 20-mesh sieve to obtain bone particles. The bone particles were added to a Soxhlet extractor, and petroleum ether was used as the extraction solvent. Reflux extraction was performed for 1-5 hours to obtain defatted bone particles. The defatted bone particles were added to an EDTA solution with a mass concentration of 5%, and the mass ratio of the material to the liquid was 1:10. Stirring and decalcifying were carried out for 36 hours. During this period, fresh EDTA solution was replaced every 6 hours. After decalcification, the precipitate was collected and fully washed with deionized water and dried to obtain defatted and decalcified bone particles. The defatted and decalcified bone particles were added to deionized water at a mass ratio of 1:10, and 5% and 1% of organic acid and pepsin were added to the defatted and decalcified bone particles, respectively. The organic acid consisted of citric acid and glacial acetic acid in a mass ratio of 4:10. After continuous stirring for 24 hours at 4°C, the enzyme was inactivated and centrifuged. The clear liquid was collected and filtered with a 3kDa ultrafiltration membrane. The filtrate was collected and freeze-dried to obtain a crude collagen peptide. The crude collagen peptide The mixed solution of 0.05 g / ml is obtained by adding 10% ethanol aqueous solution, and the mixed solution is transported to a fixed bed reactor at a flow rate of 0.5 ml / min for deodorization through a high-pressure constant flow pump. The fixed bed reactor is filled with 1-sulfonic acid butyl-3-methylimidazole hydrogen sulfate / SBA-15 molecular sieve, the bed height is 5 cm, the deodorization temperature is 70°C, the reaction liquid after deodorization is collected, filtered again with a 3kDa ultrafiltration membrane, and spray-dried at 140°C to obtain a finished collagen peptide product. The trichloroacetic acid soluble polypeptide method is used to measure the short peptide yield of 51.3%. The hydrolysis of collagen is that the peptide bonds in the molecule are destroyed and broken, and the acid can attack the peptide bonds to break them. Lauric acid contains long alkyl chains, and the addition of lauric acid may cause the spatial structure of the collagen to change during the hydrolysis process, thereby improving the efficiency of the acid action and increasing the target sites of the enzyme, thereby improving the enzymatic efficiency and the yield.

[0048] Wherein, the preparation method of 1-sulfonic acid butyl-3-methylimidazole hydrogen sulfate / SBA-15 molecular sieve is the same as that in Example 1.

[0049] Comparative Example 2:

[0050] The method is basically the same as Example 1, except that the crude collagen peptide is deodorized by using activated carbon. The specific method is as follows:

[0051] The crude collagen peptide was added to deionized water to obtain a 0.05 g / ml solution, and powdered activated carbon was added at 1% of the mass of the crude collagen peptide. The mixture was heated to 70°C in an ultrasonic water bath and stirred for reaction for 1 hour before filtering. The filtrate was filtered again with a 3 kDa ultrafiltration membrane and spray-dried at 140°C to obtain the finished collagen peptide. The short peptide yield was measured by the trichloroacetic acid soluble polypeptide method to be 34.6%.

[0052] Comparative Example 3:

[0053] The method is basically the same as Example 1, except that only 1-sulfonic acid butyl-3-methylimidazole hydrogen sulfate is loaded in the fixed bed reactor, that is, SBA-15 molecular sieve is not used as a carrier.

[0054] This comparative example provides a method for preparing a collagen peptide that can effectively delay skin aging:

[0055] Fresh horse tibia was thoroughly washed with deionized water and then dried. After being crushed, it was passed through a 20-mesh sieve to obtain bone particles. The bone particles were added to a Soxhlet extractor, and petroleum ether was used as the extraction solvent. Reflux extraction was performed for 1-5 hours to obtain defatted bone particles. The defatted bone particles were added to an EDTA solution with a mass concentration of 5% at a material-liquid mass ratio of 1:10. The solution was continuously stirred for decalcification for 36 hours, during which fresh EDTA solution was replaced every 6 hours. After decalcification, the precipitate was collected and thoroughly washed with deionized water and dried to obtain defatted and decalcified bone particles. The defatted and decalcified bone particles were added to deionized water at a mass ratio of 1:10, and organic acid and pepsin of 5% and 1% by mass of the defatted and decalcified bone particles were added respectively. The organic acid consisted of lauric acid, citric acid and glacial acetic acid in a mass ratio of 1:4:10. After the enzymatic hydrolysis was continuously stirred at ℃ for 24 hours, the enzyme was inactivated and centrifuged, the clear liquid was collected and filtered through a 3 kDa ultrafiltration membrane, the filtrate was collected and freeze-dried to obtain a crude collagen peptide, the crude collagen peptide was added to an ethanol aqueous solution with a mass concentration of 10% to obtain a 0.05 g / ml mixed solution, and the mixed solution was transported to a fixed bed reactor at a flow rate of 0.5 ml / min for deodorization by a high-pressure constant flow pump, the fixed bed reactor was filled with 1-sulfonic acid butyl-3-methylimidazole hydrogen sulfate, the bed height was 5 cm, the deodorization temperature was 70 ℃, the reaction solution after deodorization was collected and filtered through a 3 kDa ultrafiltration membrane again and spray-dried at 140 ℃ to obtain a finished collagen peptide, and the short peptide yield was 57.7% measured by the trichloroacetic acid soluble polypeptide method.

[0056] Performance testing:

[0057] ① The crude collagen peptides and finished collagen peptides prepared in Examples 1-5 and Comparative Examples 1-3 of the present invention were subjected to olfactory perception judgment, wherein the olfactory perception judgment criteria are shown in Table 1 below:

[0058] Table 1:

[0059]

[0060] The test results are shown in Table 2 below:

[0061] Table 2:

[0062]

[0063] It can be seen from Table 2 that, compared with activated carbon deodorization, the finished collagen peptide product prepared by the method of the present invention can be completely odorless.

[0064] ② Take 110 clean-grade 3-month-old female ICR mice, feed and drink freely, and keep natural light. The mice were randomly divided into 11 groups (10 in each group): respectively recorded as normal group, model group, experimental group 1-9, the normal group was injected with normal saline subcutaneously in the back of the neck every day as a control, and the other groups were injected with D-galactose (1200 mg / kg bw) to build a subacute aging model. The normal group and the model group were gavaged with normal saline every day, and the experimental groups 1-9 were gavaged with the finished collagen peptides prepared in Examples 1-5 and Comparative Examples 1-3 and commercially available collagen (Xi'an Ruierli Biological) (500 mg / kg bw) for 8 consecutive weeks. After the last gavage, the mice were fasted for 12 hours, the eyeballs were removed and blood was taken to kill, and SOD, GSH-P were tested according to the instructions of the kit. x Activity and MDA content.

[0065] The above test results are shown in Table 3 below:

[0066] Table 3:

[0067]

[0068] The free radical theory is one of the internationally recognized theories of aging. Reducing the production of free radicals and increasing the activity of antioxidant enzymes have become effective methods to delay skin aging. x As the main enzyme for removing free radicals in the body, it is used as an important reference index in the study of delaying aging. As can be seen from Table 3 above, the SOD and GSH-P levels in the skin of the model group mice x The activity was significantly reduced, and the MDA content was significantly increased, indicating that the antioxidant capacity of the mouse skin in the model group was decreased, the generation of free radicals increased, and the intermolecular cross-linking was aggravated. The SOD and GSH-P levels of the mouse skin in the experimental groups 1-9 were significantly decreased. x The activity was significantly higher than that of the model group, and the MDA content was significantly lower than that of the model group, which shows that collagen peptides can delay skin aging by increasing the activity of antioxidant enzymes. Moreover, by comparison, it can be seen that the anti-aging activity of the collagen peptides prepared by the present invention is better than that of commercially available collagen peptides.

[0069] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit the same. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that the technical solutions described in the aforementioned embodiments may still be modified, or some of the technical features may be replaced by equivalents. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A method for preparing a collagen peptide that can effectively delay skin aging, characterized in that: The details are as follows: The animal bones are washed, dried and crushed to obtain bone particles. The bone particles are defatted and decalcified, and then organic acid and protease are added for enzymolysis. After the enzymolysis is completed, the enzyme is inactivated, the clear liquid is collected and filtered with a 3kDa ultrafiltration membrane, the filtrate is collected and freeze-dried to obtain a crude collagen peptide, the crude collagen peptide is added to an ethanol aqueous solution to obtain a mixed solution, the mixed solution is transported to a reactor filled with an ionic liquid by a high-pressure constant flow pump for deodorization, the deodorized reaction liquid is collected, filtered again with a 3kDa ultrafiltration membrane and spray-dried.

2. The method for preparing the collagen peptide capable of effectively delaying skin aging according to claim 1, characterized in that: The method of degreasing is as follows: Add the bone particles into a Soxhlet extractor, use petroleum ether as the extraction solvent, and reflux extraction for 1-5 hours.

3. The method for preparing the collagen peptide capable of effectively delaying skin aging according to claim 1, characterized in that: The decalcification method is as follows: Add the defatted bone particles into the EDTA solution and continue to stir and decalcify for 16-40 hours. During this period, replace the fresh EDTA solution every 4-8 hours. After decalcification, collect the precipitate, wash it, and dry it.

4. The method for preparing the collagen peptide capable of effectively delaying skin aging according to claim 1, characterized in that: The organic acid is a combination of lauric acid, citric acid and glacial acetic acid; The mass ratio of the lauric acid, citric acid and glacial acetic acid is 1-10:1-10:1-10.

5. The method for preparing the collagen peptide capable of effectively delaying skin aging according to claim 1, characterized in that: The protease is any one or a combination of two or more of pepsin, alkaline protease, neutral protease, papain and trypsin.

6. The method for preparing the collagen peptide capable of effectively delaying skin aging according to claim 1, characterized in that: The ionic liquid is supported on the molecular sieve.

7. The method for preparing the collagen peptide capable of effectively delaying skin aging according to claim 6, characterized in that: The molecular sieve is SBA-15 molecular sieve.

8. The method for preparing the collagen peptide capable of effectively delaying skin aging according to claim 1, characterized in that: The ionic liquid has a dual acidic group.

9. The method for preparing the collagen peptide capable of effectively delaying skin aging according to claim 8, characterized in that: The ionic liquid is 1-sulfonic acid butyl-3-methylimidazole hydrogen sulfate.

10. A collagen peptide that can effectively delay skin aging, characterized in that: The method is prepared according to any one of claims 1 to 9.

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