Method for cultivating new variety of high-quality stress-resistant Lanzhou honey dew melon
By selecting suitable parents for artificial pollination and hybridization, combining mutagenesis and biotechnology breeding, new varieties of white orchids with stress resistance are obtained, which solves the weaknesses of white orchids in adverse environments and pests and diseases, and improves yield and quality.
Patent Information
- Application Number
- CN202510083721.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-01-20
- Publication Date
- 2025-03-14
- Estimated Expiration
- Not applicable · inactive patent
AI Technical Summary
During the growth process, white orchids are easily affected by adverse environments and pests, resulting in difficulty in growing seedlings, weak seedlings, and weak disease resistance, which in turn affects yield and quality.
By determining the desired stress resistance traits, traditional high-quality white orchid varieties are selected as the parent and foreign melon varieties with stress resistance or local varieties screened as the parent, artificial pollination and hybridization are carried out, combined with mutagenesis methods or biotechnology-assisted breeding, plants with stress resistance are obtained, and planting and setting field management are carried out.
A new variety of high-quality and stress-resistant Lanzhou white orchid has been cultivated, which has the ability to resist drought, low temperature, and disease and pests, and has improved the yield and quality of white orchid.
Smart Images
Figure CN119605639A_ABST
Abstract
Description
Technical Field
[0001] The invention relates to the technical field of white honeydew melon cultivation, and in particular to a method for cultivating a new variety of high-quality stress-resistant Lanzhou white honeydew melon. Background Art
[0002] White melon, also known as Lanzhou honeydew melon, formerly known as "honeydew", is native to the Red Sea and Jingdong areas of France and the United States. It was introduced to Lanzhou from the United States in 1944. It has thick skin, sweet melon, emerald green flesh, thick sac and rich juice, crisp and tender, with a sugar content of about 15%. It enjoys the reputation of "fragrant as osmanthus and sweet as honey". It is not only sweet and delicious, rich in nutrition, but also has the effects of clearing away heat, quenching thirst, appetizing and diuretic. It is not only supplied to all parts of the country, but also exported overseas, so its output is often in short supply; in the growth process of white melon, there will be adverse environment and pests and diseases. The main diseases are wilt, powdery mildew, bacterial angular spot, downy mildew, and the pests are whiteflies, American spots, leafminers, etc., all of which will lead to difficulties in white melon seedling cultivation. The cultivated seedlings are weak and prone to damping-off disease. The plants have weak disease resistance and are prone to diseases, which greatly affects the yield and quality. Summary of the invention
[0003] The invention provides a method for breeding a high-quality stress-resistant Lanzhou white melon new variety, which is used to breed the stress-resistant Lanzhou white melon new variety, achieve the desired stress resistance such as drought resistance, low temperature resistance, and disease and insect pest resistance, and improve the yield and quality of the white melon.
[0004] The present invention provides a method for breeding a new variety of high-quality stress-resistant Lanzhou white melon, comprising:
[0005] S1. Determine the desired stress resistance traits, select a traditional high-quality honeydew melon variety as the female parent, and select an exotic melon variety with stress resistance or a local variety screened for stress resistance as the male parent; wherein the stress resistance traits include drought resistance, low temperature resistance, and disease and insect pest resistance;
[0006] S2. During the flowering period of the white melon, select a sunny morning to collect pollen from the male parent, collect it in a clean container, then dip the pollen with a brush or a pollination stick, and gently apply it on the stigma of the female parent's flower to complete artificial pollination and harvest white melon hybrid seeds;
[0007] S3, breeding the white melon hybrid seeds by using a mutagenesis method or a biotechnology-assisted method to obtain plants with stress resistance; wherein the mutagenesis method includes physical mutagenesis and chemical mutagenesis, and the biotechnology-assisted method includes genetic engineering and molecular markers;
[0008] S4, planting the stress-resistant plants and managing them using a set field management method; wherein the field management includes temperature management, light management, fertilizer and water management, reasonable pruning and pollination to retain melons;
[0009] S5. Carry out self-pollination for multiple generations of plants with stress resistance to stabilize their genetic traits.
[0010] Furthermore, in step S3, the physical mutagenesis is to perform mutagenesis treatment on the honey melon hybrid seeds using physical factors such as gamma rays, X-rays or ultraviolet rays; that is, the honey melon hybrid seeds are irradiated with a set dose of gamma rays, and the dose is determined based on preliminary experiments and is set between 10-50Gy; the treated honey melon hybrid seeds are sown, the mutation of their offspring is observed, and mutants with enhanced stress resistance are screened out from the mutagenized offspring.
[0011] Furthermore, in step S3, the chemical mutagenesis is to treat the honey melon hybrid seeds with a chemical mutagen ethyl methanesulfonate, soak the honey melon hybrid seeds in an ethyl methanesulfonate solution of a set concentration for a set time, then wash the seeds and sow them, and screen the plants with the target stress resistance traits among the chemically mutated offspring.
[0012] Furthermore, in step S3, the genetic engineering is as follows: first, genes related to stress resistance are determined, these genes are cloned from other plants with cold resistance through gene cloning technology, and the stress resistance genes are introduced into the genome of the white melon by Agrobacterium-mediated transformation or gene gun method. The transformed plants are subjected to molecular detection to verify whether the target genes are successfully integrated into the white melon genome, and then the stress resistance of the transgenic plants is identified.
[0013] Furthermore, in step S3, the molecular markers are: looking for molecular markers closely linked to stress resistance genes, that is, using SSR or SNP molecular marker technology, through comparative analysis of the genomes of stress-resistant and sensitive varieties of white melon, to find markers related to stress resistance, and in the breeding process, use these molecular markers to perform early screening of offspring.
[0014] Furthermore, in step S4, the plants with stress resistance are planted, including: using plug tray substrates for seedling cultivation, sowing in late January, and planting when the seedlings are 30 to 35 days old and have 3 leaves and 1 heart; in late February, the double-pole double-film greenhouse is closed and the greenhouse is sealed for 10 to 15 days; in early March, when the ground temperature 5 cm in the greenhouse is stable above 12°C, planting can be carried out, using wide and narrow rows for planting, with a wide row of 80 cm and a narrow row of 30 cm, covering the wide row with a 100 cm wide film, transplanting in a "T" shape, planting 2 rows per film, with a plant spacing of 65 cm, 1 plant per hole, and maintaining 27,000 seedlings / hm 2 , build a small shed after planting.
[0015] Further, in step S4, temperature management: early spring cultivation is mainly based on heat preservation and light enhancement. The inner film of the double-pole double-film plastic greenhouse is lowered after sunset to reduce heat energy loss and meet the temperature conditions required by crops. After planting, a small arch shed is used inside the greenhouse for nighttime heat preservation. When the melon vine grows to about 30 cm, the small arch shed is no longer covered at night; the daytime temperature during the seedling stage is 28-32°C and the nighttime temperature is 18-20°C; the daytime temperature during the vine extension stage is 25-30°C and the nighttime temperature is 15-18°C; the daytime temperature during the fruit growth stage is 28-32°C and the nighttime temperature is 15-18°C;
[0016] Light management: After sunrise, use a film roller to roll up the inner greenhouse film to increase the light transmittance of the greenhouse for solar energy conversion and accumulation. Clean the outer greenhouse film regularly to increase the light transmittance of the greenhouse.
[0017] Fertilizer and water management: 7-10 days after transplanting, choose a sunny day to water the seedlings to slow down the growth, 20 days after transplanting, water the vines to stretch them; after the fruit is set, water the first swelling water, and apply 75-150kg / hm2 of water-soluble fruit swelling fertilizer with the water. 2 20 days after pollination, water the fruit for the second time and apply 75kg / hm2 of water-soluble fruit-swelling fertilizer 2 , stop watering 7 days before picking and marketing.
[0018] Further, in step S4, reasonable pruning is performed: adopting climbing vine cultivation and 12-vine pruning method, that is, when the main vine grows to 8 leaves, the top is pinched, 4 strong sub-vines are selected, and the sub-vines are pinched when they grow to 4-5 leaves, and 3 grandchildren are selected for each sub-vine, the first grandchild vine near the base is pinched with 3 leaves, the second grandchild vine is pinched with 2 leaves, and the third grandchild vine is pinched with 1 leaf, and pruning should be performed on a sunny day after the dew has dried;
[0019] Pollination: When the melon is flowering, artificial pollination can be used, or 0.1% chlorfenapyr soluble liquid 75mL / hm2 can be used on the day of flowering or 1-2 days before flowering. 2 Add 37.5kg of water and spray the melon embryos to promote fruit setting. The concentration of chlormequat should be appropriate and spray evenly. Select the young melons on the second vine to keep.
[0020] The beneficial effects of the present invention are:
[0021] The present invention first determines the desired stress resistance trait, selects a traditional high-quality white melon variety as the female parent, and selects a foreign melon variety with stress resistance or a local variety screened for stress resistance as the male parent; then, during the flowering period of the white melon, collects pollen from the male parent and dips the pollen to gently smear it on the stigma of the female parent's flower to complete artificial pollination and harvest white melon hybrid seeds; then, the white melon hybrid seeds are bred using a mutagenesis method or a biotechnology-assisted method to obtain stress-resistant plants; finally, the stress-resistant plants are planted, and managed using a set field management method, and the planted stress-resistant plants are self-pollinated for multiple generations to stabilize their genetic traits. The cultivated stress-resistant Lanzhou white melon new variety achieves the desired stress resistance shape, such as drought resistance, low temperature resistance, and disease and insect resistance, and achieves the effect of improving the yield and quality of the white melon. BRIEF DESCRIPTION OF THE DRAWINGS
[0022] Figure 1 The present invention is a schematic diagram of the process of breeding a new variety of high-quality stress-resistant Lanzhou white melon.
[0023] The realization of the purpose, functional features and advantages of the present invention will be further explained in conjunction with embodiments and with reference to the accompanying drawings. DETAILED DESCRIPTION
[0024] It should be understood that the specific embodiments described herein are only used to explain the present invention, and are not used to limit the present invention.
[0025] like Figure 1 As shown, the present invention provides a method for breeding a new variety of high-quality stress-resistant Lanzhou white melon, comprising:
[0026] S1. Determine the desired stress resistance traits, and select a traditional high-quality honeydew melon variety as the female parent, and select an exotic melon variety with stress resistance or a local variety that has been screened for stress resistance as the male parent; wherein the stress resistance traits include drought resistance, low temperature resistance, and disease and insect pest resistance.
[0027] For Lanzhou white melon, resistance to powdery mildew and downy mildew are relatively important disease resistance traits, because these diseases are more common in the local planting environment. At the same time, fruit quality-related traits such as sweetness, crispness, fruit shape, and peel thickness should also be considered. Select traditional high-quality white melon varieties as the female parent, which have good fruit quality, such as high sweetness and crisp and tender flesh; select foreign melon varieties with strong stress resistance or local varieties screened for stress resistance as the male parent, which may carry genes for resistance to diseases and pests or adverse environments. Select parents with large differences in genetic background for hybridization to increase the possibility of hybrid vigor. White melon variety resources can be collected from different ecological regions, including wild relatives, to broaden the genetic basis of the parents.
[0028] S2. During the flowering period of honeydew melon, choose a sunny morning to collect pollen from the male parent first, collect it in a clean container, then dip a brush or pollination stick into the pollen and gently apply it on the stigma of the female parent's flower to complete artificial pollination and harvest honeydew melon hybrid seeds.
[0029] In order to ensure successful pollination, the pollinated flowers can be marked for subsequent observation of fruit development. Generally, the pollen of a male flower can pollinate multiple female flowers. After harvesting the hybrid seeds, they can be sown directly. In the first generation of hybrids (F1), the main observations are their growth potential, stress resistance and other performances. For example, observe the response of the plants to drought stress, and compare the growth conditions of the hybrid offspring with those of the parents under the same drought conditions, including the degree of leaf wilting and the growth rate of the plants. For fruit quality traits, they can be evaluated after the fruit is ripe. For example, use a refractometer to measure the sweetness of the fruit, use a caliper to measure the fruit shape index, and select individuals with both stress resistance and excellent fruit quality.
[0030] S3. Breeding the honey melon hybrid seeds by induction of mutation or by biotechnology-assisted breeding to obtain stress-resistant plants; wherein the induction of mutation includes physical mutagenesis and chemical mutagenesis, and the biotechnology-assisted breeding includes genetic engineering and molecular markers.
[0031] Physical mutagenesis: Use physical factors such as gamma rays, X-rays or ultraviolet rays to induce mutagenesis on white melon seeds or seedlings. For example, irradiate seeds with a set dose of gamma rays. The dose is generally determined based on preliminary experiments and is usually between 10-50Gy. The treated seeds can be sown and the mutations of their offspring can be observed. Mutants with enhanced stress resistance, such as plants with improved tolerance to saline-alkali land, are screened out from the induced offspring, and the growth and survival of the plants are observed by planting them in an artificially simulated saline-alkali land environment.
[0032] Chemical mutagenesis: Treat honey melon seeds with chemical mutagens such as ethyl methanesulfonate (EMS). Soak the seeds in an EMS solution of a set concentration (generally 0.1%-1%) for a set time (usually a few hours to a day), then wash the seeds and sow them. Similarly, screen the chemically mutated offspring for plants with target stress resistance traits, such as plants resistant to high temperature stress, by setting a high temperature environment (such as 35-40°C during the day) to observe their growth and development, such as leaf color changes, fruit setting rate and other indicators to judge stress resistance.
[0033] Genetic engineering: Identify genes related to stress resistance. For example, the cold-resistant genes of some plants can improve the tolerance of plants to low temperatures. Through gene cloning technology, these genes are cloned from other cold-resistant plants. The stress-resistant genes are introduced into the genome of the white melon using methods such as Agrobacterium-mediated transformation or gene gun. The transformed plants are tested by molecular detection (such as PCR detection) to verify whether the target gene has been successfully integrated into the white melon genome, and then the transgenic plants are identified for stress resistance. For example, the white melon plants introduced with salt-resistant genes are planted in soil containing a certain amount of salt to observe their growth and physiological indicators, such as salt damage symptoms on the leaves and plant biomass.
[0034] Molecular markers: Look for molecular markers that are closely linked to stress resistance genes, such as using molecular marker technologies such as SSR (simple sequence repeats) or SNP (single nucleotide polymorphisms), and find markers related to stress resistance through comparative analysis of the genomes of stress-resistant and sensitive varieties of white melon. In the breeding process, these molecular markers are used for early screening of offspring. For example, at the seedling stage, by extracting DNA for molecular marker testing, it is possible to preliminarily determine which seedlings may carry stress resistance genes, thereby reducing the workload of field screening and improving breeding efficiency.
[0035] S4. Planting stress-resistant plants and managing them using a set field management method; wherein the field management includes temperature management, light management, fertilizer and water management, reasonable pruning and pollination to retain melons.
[0036] Land preparation and fertilization: Choose medium sand fields that have been sanded for 5 to 10 years. Generally, clean the fields after the autumn crops are harvested in the previous year, and apply 75,000 kg / hm2 of fully decomposed organic fertilizer after sanding. 2 , Potassium sulfate type ternary compound fertilizer 450kg / hm 2 , microbial fertilizer 750kg / hm 2 , medium and trace element fertilizer 75kg / hm 2 After application, deep plowing, leveling and compacting, and sufficient winter watering.
[0037] Planting of stress-resistant plants:
[0038] Seedlings were grown in trays, sown in late January, and transplanted when the seedlings were 30-35 days old and had 3 leaves and 1 heart. In late February, the double-pole double-film greenhouse was covered and sealed for 10-15 days. In early March, when the ground temperature at 5 cm inside the greenhouse was stable above 12°C, transplanting was carried out. Wide and narrow rows were used for transplanting, with a wide row of 80 cm and a narrow row of 30 cm. A 100 cm wide film was covered on the wide row, and transplanted in a "T" shape. Two rows were planted per film, with a spacing of 65 cm between plants, one plant per hole, and 27,000 seedlings / hm2 were guaranteed. 2 , build a small shed after planting.
[0039] Temperature management: Early spring cultivation focuses on insulation and light enhancement. The inner film of the double-pole double-film plastic greenhouse is lowered after sunset to reduce heat loss and meet the temperature conditions required by crops. After transplanting, a small arch shed is used inside the greenhouse for night insulation. When the vines grow to about 30 cm, the small arch shed is no longer covered at night; the daytime temperature during the seedling stage is 28-32°C, and the nighttime temperature is 18-20°C; the daytime temperature during the vine extension stage is 25-30°C, and the nighttime temperature is 15-18°C; the daytime temperature during the fruit growth period is 28-32°C, and the nighttime temperature is 15-18°C.
[0040] Light management: After sunrise, use a film roller to roll up the inner greenhouse film to increase the light transmittance of the greenhouse for solar energy conversion and accumulation, and clean the outer greenhouse film regularly to increase the light transmittance of the greenhouse.
[0041] Fertilizer and water management: 7-10 days after transplanting, choose a sunny day to water the seedlings to slow down the growth, 20 days after transplanting, water the vines to stretch them; after the fruit is set, water the first swelling water, and apply 75-150kg / hm2 of water-soluble fruit swelling fertilizer with the water. 2 20 days after pollination, water the fruit for the second time and apply 75kg / hm2 of water-soluble fruit-swelling fertilizer 2 In order to ensure quality and reduce fruit cracking, no watering is done 7 days before picking and marketing.
[0042] Reasonable pruning: Adopt climbing vine cultivation and 12-vine pruning method, that is, pinch off the main vine when it grows to 8 leaves, select 4 strong sub-vines, pinch off the sub-vines when they grow to 4-5 leaves, and select 3 grandchildren from each sub-vine. Pinch off the first grandchildren near the base when they have 3 leaves, pinch off the second grandchildren when they have 2 leaves, and pinch off the third grandchildren when they have 1 leaf. Pruning should be done on a sunny day after the dew has dried up to prevent slow wound healing and disease on rainy days or when there is dew.
[0043] Pollination: When the melon is flowering, artificial pollination can be used, or 0.1% chlorfenapyr soluble liquid 75mL / hm2 can be used on the day of flowering or 1-2 days before flowering. 2 Add 37.5kg of water and spray the melon embryos to promote fruit setting. The concentration of chlormequat should be appropriate and spray evenly. Select the young melons on the second vine to keep, which can take into account maturity, yield and quality. Keep one melon on each plant.
[0044] S5. Carry out self-pollination for multiple generations of plants with stress resistance to stabilize their genetic traits.
[0045] For plants with stress resistance and good quality obtained through hybridization, mutagenesis or biotechnology, multiple generations of self-pollination are required to stabilize their genetic traits. During the self-pollination process, each generation must undergo strict stress resistance and quality testing. If a specific stress resistance gene needs to be transferred from the donor parent to a high-quality honey melon variety, backcrossing can also be used. For example, plants with powdery mildew resistance genes are backcrossed with high-quality honey melon varieties. After multiple generations of backcrossing and selection, the offspring will acquire the ability to resist powdery mildew while maintaining high-quality fruit quality.
[0046] It should be noted that, in this article, the terms "include", "comprises" or any other variations thereof are intended to cover non-exclusive inclusion, so that a process, device, article or method including a series of elements includes not only those elements, but also includes other elements not explicitly listed, or also includes elements inherent to such process, device, article or method. In the absence of further restrictions, an element defined by the sentence "includes a ..." does not exclude the presence of other identical elements in the process, device, article or method including the element.
[0047] The above description is only a preferred embodiment of the present invention, and does not limit the patent scope of the present invention. Any equivalent structure or equivalent process transformation made by using the contents of the present invention specification and drawings, or directly or indirectly applied in other related technical fields, are also included in the patent protection scope of the present invention.
Claims
1. A method for breeding a new variety of high-quality stress-resistant Lanzhou white melon, characterized in that: include: S1. Determine the desired stress resistance traits, select a traditional high-quality honeydew melon variety as the female parent, and select an exotic melon variety with stress resistance or a local variety screened for stress resistance as the male parent; wherein the stress resistance traits include drought resistance, low temperature resistance, and disease and insect pest resistance; S2. During the flowering period of the white melon, select a sunny morning to collect pollen from the male parent, collect it in a clean container, then dip the pollen with a brush or a pollination stick, and gently apply it on the stigma of the female parent's flower to complete artificial pollination and harvest white melon hybrid seeds; S3, breeding the white melon hybrid seeds by using a mutagenesis method or a biotechnology-assisted method to obtain plants with stress resistance; wherein the mutagenesis method includes physical mutagenesis and chemical mutagenesis, and the biotechnology-assisted method includes genetic engineering and molecular markers; S4, planting the stress-resistant plants and managing them using a set field management method; wherein the field management includes temperature management, light management, fertilizer and water management, reasonable pruning and pollination to retain melons; S5. Carry out self-pollination for multiple generations of plants with stress resistance to stabilize their genetic traits.
2. The method for breeding a new variety of high-quality stress-resistant Lanzhou white melon according to claim 1, characterized in that: In step S3, the physical mutagenesis is to perform mutagenesis treatment on the honey melon hybrid seeds using physical factors such as gamma rays, X-rays or ultraviolet rays; that is, irradiating the honey melon hybrid seeds with a set dose of gamma rays, the dose is determined according to preliminary experiments and is set between 10-50Gy; sowing the treated honey melon hybrid seeds, observing the mutation of their offspring, and screening out mutants with enhanced stress resistance from the induced offspring.
3. The method for breeding a new variety of high-quality stress-resistant Lanzhou white melon according to claim 1, characterized in that: In step S3, the chemical mutagenesis is to treat the honey melon hybrid seeds with a chemical mutagen ethyl methanesulfonate, soak the honey melon hybrid seeds in an ethyl methanesulfonate solution of a set concentration for a set time, then wash the seeds and sow them, and screen the plants with the target stress resistance trait among the chemically mutagenized offspring.
4. The method for breeding a new variety of high-quality stress-resistant Lanzhou white melon according to claim 1, characterized in that: In step S3, the genetic engineering is as follows: first, genes related to stress resistance are determined, these genes are cloned from other plants with cold resistance through gene cloning technology, and stress resistance genes are introduced into the genome of white melon using Agrobacterium-mediated transformation or gene gun method. After the transformation, molecular detection is performed on the transformed plants to verify whether the target genes are successfully integrated into the genome of the white melon, and then the stress resistance of the transgenic plants is identified.
5. The method for breeding a new variety of high-quality stress-resistant Lanzhou white melon according to claim 1, characterized in that: In step S3, the molecular markers are: looking for molecular markers that are closely linked to stress resistance genes, that is, using SSR or SNP molecular marker technology, through comparative analysis of the genomes of stress-resistant and sensitive varieties of white melon, to find markers related to stress resistance, and in the breeding process, use these molecular markers to perform early screening of offspring.
6. The method for breeding a new variety of high-quality stress-resistant Lanzhou white melon according to claim 1, characterized in that: In the step S4, the colonization of stress-resistant plants includes: using plug tray substrate for seedling raising, sowing in late January, with a seedling age of 30 - 35 days, and colonizing when there are three leaves and one heart; covering the double-rod double-film greenhouse in late February and fumigating the greenhouse for 10 - 15 days; in early March, colonizing can be carried out when the soil temperature at 5 cm in the greenhouse is stable above 12°C. Colonize in wide and narrow rows, with a wide row of 80 cm and a narrow row of 30 cm. Cover a plastic film with a width of 100 cm on the wide row, transplant in a "T" shape, plant 2 rows per film, with a plant spacing of 65 cm, 1 plant per hole, and keep 27,000 plants per hectare. 2 After colonization, cover with a small arch shed.
7. The method for breeding a new variety of high-quality stress-resistant Lanzhou white melon according to claim 1, characterized in that: In step S4, temperature management: early spring cultivation is mainly based on heat preservation and light enhancement. The inner film of the double-pole double-film plastic greenhouse is lowered after sunset to reduce heat energy loss and meet the temperature conditions required by crops. After planting, a small arch shed is used inside the greenhouse for nighttime heat preservation. When the melon vine grows to about 30 cm, the small arch shed is no longer covered at night; the daytime temperature during the seedling stage is 28-32°C and the nighttime temperature is 18-20°C; the daytime temperature during the vine extension stage is 25-30°C and the nighttime temperature is 15-18°C; the daytime temperature during the fruit growth stage is 28-32°C and the nighttime temperature is 15-18°C; Light management: After sunrise, use a film roller to roll up the inner greenhouse film to increase the light transmittance of the greenhouse for solar energy conversion and accumulation. Clean the outer greenhouse film regularly to increase the light transmittance of the greenhouse. Fertilizer and water management: 7-10 days after transplanting, choose a sunny day to water the seedlings to slow down the growth, 20 days after transplanting, water the vines to stretch them; after the fruit is set, water the first swelling water, and apply 75-150kg / hm2 of water-soluble fruit swelling fertilizer with the water. 2 20 days after pollination, water the fruit for the second time and apply 75kg / hm2 of water-soluble fruit-swelling fertilizer 2 , stop watering 7 days before picking and marketing.
8. The method for breeding a new variety of high-quality stress-resistant Lanzhou white melon according to claim 1, characterized in that: In the step S4, reasonable pruning: adopting climbing vine cultivation and 12-vine pruning method, that is, pinching when the main vine grows to 8 leaves, selecting and retaining 4 strong sub-vines, pinching when the sub-vines grow to 4-5 leaves, and selecting and retaining 3 grandchildren from each sub-vine, pinching the first grandchildren near the base with 3 leaves, pinching the second grandchildren with 2 leaves, and pinching the third grandchildren with 1 leaf. Pruning should be carried out on a sunny day after the dew has dried up; Pollination: When the melon is flowering, artificial pollination can be used, or 0.1% chlorfenapyr soluble liquid 75mL / hm2 can be used on the day of flowering or 1-2 days before flowering. 2 Add 37.5kg of water and spray the melon embryos to promote fruit setting. The concentration of chlormequat should be appropriate and spray evenly. Select the young melons on the second vine to keep.