Composition with anti-wrinkle effect and application of composition in preparation of cosmetics

Abstract

The invention belongs to the technical field of cosmetics, and provides a composition with an anti-wrinkle effect and application of the composition in preparation of cosmetics. The composition provided by the invention comprises a mulberry root extract and a recombinant human collagen III, and the mass part ratio of the mulberry root extract to the recombinant human collagen III is (2-20): 1. In the composition provided by the invention, the mulberry root extract and the recombinant human collagen III have a synergistic anti-wrinkle effect, and the effect of the composition is obviously greater than the sum of the effects of singly using the mulberry root extract and singly using the recombinant human collagen III in the aspect of promoting the synthesis of the skin fibroblast collagen I. Therefore, when the composition with the anti-wrinkle effect provided by the invention is used for preparing cosmetics, the obvious anti-wrinkle effect can be obtained under the conditions of reducing the use amount of the recombinant human collagen III and saving the cost.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and in particular to a composition with anti-wrinkle effect and application thereof in preparing cosmetics. Background Art

[0002] As we age, our skin begins to age due to external factors such as long-term exposure to the sun or ultraviolet radiation, microbial invasion, chemical substances, physical damage, etc. At the same time, with the deepening of cosmetic science research and the improvement of living standards, consumers' demand for functional skin care products is growing, and anti-aging has become one of the main demands of consumers for skin care.

[0003] During the aging process of the skin, collagen loss becomes faster and faster, especially after the age of 25. The synthesis rate of type I and III collagen in the skin cannot keep up with the loss rate, resulting in wrinkles, sagging and other aging phenomena on the skin. Recombinant human collagen can directly supplement collagen for the skin and stimulate the skin to produce collagen. Compared with retinol or vitamin C, it has better safety and is non-irritating. However, recombinant human collagen III is currently expensive, resulting in higher costs and prices for cosmetics using this ingredient. Summary of the invention

[0004] The object of the present invention is to provide a composition with anti-wrinkle effect and its application in the preparation of cosmetics, so as to achieve significant anti-wrinkle effect with a lower amount of recombinant human collagen III.

[0005] In order to achieve the above object, the present invention adopts the following technical solutions:

[0006] A first aspect of the present invention provides a composition with anti-wrinkle effect, which comprises mulberry root extract and recombinant human collagen III, wherein the mass ratio of the mulberry root extract to the recombinant human collagen III is (2-20):1.

[0007] Preferably, in the anti-wrinkle composition provided by the present invention, the mass ratio of the mulberry root extract to the recombinant human collagen III is (2-4):1.

[0008] In the composition with anti-wrinkle effect provided by the present invention, the mulberry root extract is prepared by a method comprising the following steps: (1) taking the root of a plant of the species white mulberry or black mulberry, crushing and drying to obtain an extraction raw material; (2) immersing the extraction raw material in a solvent at a ratio of 0.05kg / L to 0.1kg / L, and leaching for 36 to 60 hours under stirring to obtain an extract; the solvent is selected from ethanol, propylene glycol, butylene glycol or aqueous solutions of the above solvents; (3) filtering and decolorizing the extract to obtain the mulberry root extract.

[0009] Preferably, in the preparation step of the mulberry root extract provided by the present invention, the solvent is an aqueous solution of 1,3-propylene glycol with a volume percentage concentration of 70 to 90%.

[0010] In the anti-wrinkle composition provided by the present invention, the recombinant human collagen III is composed of a short amino acid sequence from natural human collagen III as a basic repeating unit, which is repeated multiple times; the basic repeating unit is gergapgfrgpagpngipgekgpagergap, and the number of repetitions of the basic repeating unit is 1 to 16.

[0011] In the anti-wrinkle composition provided by the present invention, the molecular weight of the recombinant human collagen III is 3-50KD.

[0012] The second aspect of the present invention provides use of the composition with anti-wrinkle efficacy according to the first aspect of the present invention in the preparation of cosmetics.

[0013] In the above application in preparing cosmetics, the composition with anti-wrinkle efficacy provided by the present invention synergistically increases the synthesis of collagen I in fibroblasts.

[0014] The third aspect of the present invention provides a cosmetic comprising the composition with anti-wrinkle efficacy according to the first aspect of the present invention.

[0015] Preferably, in the cosmetics provided by the present invention, the mass percentage concentration of the mulberry root extract in the cosmetics is 0.001% to 1%, and the mass percentage concentration of the recombinant human collagen III in the cosmetics is 0.0001% to 1%.

[0016] Compared with the prior art, the present invention provides a composition with anti-wrinkle efficacy, which comprises a mulberry root extract and a recombinant human collagen III in a specific mass ratio. In the combination provided by the present invention, the mulberry root extract and the recombinant human collagen III have a synergistic anti-wrinkle efficacy, and in terms of promoting the synthesis of fibroblast collagen I, the efficacy of the composition is significantly greater than the sum of the efficacy of using the mulberry root extract alone and the recombinant human collagen III alone. The composition with anti-wrinkle efficacy provided by the present invention is used in the preparation of cosmetics, which can obtain significant anti-wrinkle efficacy while reducing the amount of recombinant human collagen III used and saving costs. DETAILED DESCRIPTION

[0017] In order to make the purpose, technical scheme and advantages of the present invention clearer, the various embodiments of the present invention will be described in detail below. However, it will be appreciated by those skilled in the art that in the various embodiments of the present invention, many technical details are provided in order to enable the reader to better understand the present application. However, even without these technical details and various changes and modifications based on the following embodiments, the technical scheme claimed for protection in the claims of the present application can be implemented.

[0018] According to the first aspect of the present application, an embodiment of the present application provides a composition with anti-wrinkle effect, which comprises mulberry root extract and recombinant human collagen III, wherein the mass ratio of mulberry root extract to recombinant human collagen III is (2-20):1.

[0019] For example, the mass ratio of mulberry root extract to recombinant human collagen III can be 2:1, 3:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1, 10:1, 11:1, 12:1, 13:1, 14:1, 15:1, 16:1, 17:1, 18:1, 19:1, 20:1, or within the range of any two of the above ratios.

[0020] In an embodiment of the present application, a composition with anti-wrinkle efficacy comprises mulberry root extract and recombinant human collagen III in a mass ratio of (2 to 20): 1. Experimental verification shows that when mulberry root extract and recombinant human collagen III are compounded in a mass ratio of (2 to 20): 1, the efficacy of the obtained composition in promoting the synthesis of fibroblast collagen I is significantly greater than the sum of the efficacy of using mulberry root extract alone and recombinant human collagen III alone. Therefore, the composition provided in an embodiment of the present application has a synergistic anti-wrinkle effect. When the composition is used in the preparation of cosmetics, a significant anti-wrinkle effect can be obtained at a lower concentration of recombinant human collagen III, which can improve the efficacy of anti-wrinkle and anti-aging cosmetics and reduce the cost of anti-wrinkle and anti-aging cosmetics.

[0021] In some embodiments of the present application, the mass ratio of mulberry root extract to recombinant human collagen III is (2-4):1, thereby achieving more significant synergistic effects in promoting fibroblast collagen I synthesis and anti-wrinkle.

[0022] For example, the mass ratio of mulberry root extract to recombinant human collagen III can be 2:1, 3:1, 4:1, or within the range of any two of the above ratios.

[0023] In some embodiments of the present application, the mulberry root extract is prepared by a method comprising the following steps: (1) taking the roots of white mulberry or black mulberry species, crushing and drying to obtain an extraction raw material; (2) immersing the extraction raw material in a solvent at a ratio of 0.05kg / L to 0.1kg / L, and extracting for 36 to 60 hours under stirring to obtain an extract; the solvent used is selected from ethanol, propylene glycol, butylene glycol or an aqueous solution of the above solvents; (3) filtering and decolorizing the extract to obtain the mulberry root extract.

[0024] In some embodiments of the present application, the solvent used in the preparation step of the mulberry root extract is an aqueous solution of 1,3-propylene glycol with a volume percentage concentration of 70 to 90% to obtain a better extraction rate.

[0025] In an embodiment of the present application, the recombinant human collagen III is composed of a short amino acid sequence from natural human collagen III as a basic repeating unit, which is repeated multiple times; the basic repeating unit is gergapgfrgpagpngipgekgpagergap, and the number of repetitions of the basic repeating unit is 1 to 16.

[0026] In the embodiment of the present application, the molecular weight of recombinant human collagen III is 3 to 50 KD. When the basic repeating unit is repeated once, the molecular weight of recombinant human collagen III is about 3 KD; when the basic repeating unit is repeated 8 times, the molecular weight of recombinant human collagen III is about 25 KD; when the basic repeating unit is repeated 16 times, the molecular weight of recombinant human collagen III is about 50 KD.

[0027] According to the second aspect of the present application, the embodiments of the present application also provide the use of the composition with anti-wrinkle effect of the first aspect of the present application in the preparation of cosmetics.

[0028] In the above application in preparing cosmetics, the composition with anti-wrinkle efficacy provided by the present invention synergistically increases the synthesis of collagen I in fibroblasts.

[0029] According to the third aspect of the present application, an embodiment of the present application further provides a cosmetic comprising the composition with anti-wrinkle efficacy according to the first aspect of the present application.

[0030] In some embodiments of the present application, the mass percentage concentration of mulberry root extract in the cosmetic is 0.001% to 1%, and the mass percentage concentration of recombinant human collagen III in the cosmetic is 0.0001% to 1%.

[0031] The advantages of the present application are further described below in conjunction with specific examples. It should be understood that these examples are only used to illustrate the present application and are not used to limit the scope of the present application.

[0032] Examples 1 to 5, Comparative Examples 1 to 10

[0033] 1. Preparation or acquisition of raw materials:

[0034] (1) Preparation of mulberry root extract:

[0035] a) taking the root of white mulberry of the Moraceae plant, crushing and drying to obtain an extraction raw material;

[0036] b) immersing the extracted raw material in a solvent at a ratio of 0.08 kg / L, wherein the solvent is an aqueous solution of 1,3-propylene glycol with a volume percentage concentration of 80%, and leaching for 48 hours under stirring to obtain an extract;

[0037] c) filtering and decolorizing the extract to obtain a mulberry root extract.

[0038] (2) Obtaining recombinant human collagen III:

[0039] Recombinant human collagen III was purchased from Jinbo Biotechnology. As an example, the recombinant human collagen III in Examples 1 to 5 and related comparative examples uses a model with a molecular weight of 25K.

[0040] 2. Collagen I detection experiment:

[0041] (1) Test system: The cells used in this test are fibroblasts, which were provided by Guangdong Boxi Biotechnology Co., Ltd.

[0042] (2) Main reagents: DMEM culture medium, PBS, MTT, Collagen I ELISA kit.

[0043] (3) Main equipment: CO 2 Incubator, clean bench, ELISA reader, UVA irradiator.

[0044] (4) Test method:

[0045] a) Cell seeding: After the cells are revived, when the plating rate reaches about 60%, the cells are seeded into 6-well plates and incubated in an incubator (37°C, 5% CO 2 ) and incubate overnight.

[0046] b) Prepare samples according to the concentrations in the table below. All samples are dissolved in DMEM culture medium.

[0047] Table 1 Sample concentrations of Examples 1 to 5 and Comparative Examples 1 to 10

[0048]

[0049] c) Drug administration: When the cell plating rate in the 6-well plate reaches 50% to 60%, the drug administration is carried out in groups, with 3 replicate wells in each group. 2 mL of culture medium is added to each well of the blank control group and the UV control group, and 2 mL of culture medium containing the sample of the corresponding concentration is added to each well of the sample group. After the drug administration is completed, the 6-well plate is placed in a CO 2 Incubator (37°C, 5% CO 2 ) for 24 h.

[0050] d) UVA irradiation: According to the test groups, all groups except the blank control group were irradiated with UVA at a dose of 30 J / cm 2 After irradiation, place in CO 2 Incubator (37°C, 5% CO 2 ) and cultured for 24 h.

[0051] e) ELISA test: After the incubation, the cell culture supernatant was collected in a centrifuge tube, and Collagen I was detected and analyzed according to the operating instructions of the ELISA kit.

[0052] f) Calculation of Collagen I promotion rate: Promotion rate (%) = 100% X (average concentration of Collagen 1 in the sample group - average concentration of Collagen 1 in the UV control group) / average concentration of Collagen 1 in the UV control group.

[0053] (5) Collaborative determination:

[0054] a) To illustrate that the mulberry root extract and recombinant human collagen III in the anti-wrinkle composition provided by the present invention have a synergistic effect, rather than just a simple addition of the effects of the two components, the present invention uses the COLBY formula (SR Colby, Weeds, Vol. 15, No. 1, pp. 20-22, 1967) to calculate the theoretical Collagen I enhancement rate of the composition comprising mulberry root extract and recombinant human collagen III:

[0055] Etheory = X + Y – [(X·Y) / 100]

[0056] Wherein, X is the collagen I enhancement rate after administration of single component mulberry root extract alone;

[0057] Y is the rate of collagen I enhancement after administration of single-component recombinant human collagen III alone;

[0058] Etheoretical is the theoretical Collagen I enhancement rate after administration of the combination of mulberry root extract and recombinant human collagen III.

[0059] The E theory of Example 1 is calculated from the data of Comparative Examples 2 and 6, the E theory of Example 2 is calculated from the data of Comparative Examples 4 and 7, the E theory of Example 3 is calculated from the data of Comparative Examples 3 and 6, the E theory of Example 4 is calculated from the data of Comparative Examples 2 and 5, and the E theory of Example 5 is calculated from the data of Comparative Examples 3 and 5.

[0060] Comparative Examples 1 to 7 were single-component administrations, and E theory was not calculated.

[0061] The E theory of Comparative Example 8 is calculated from the data of Comparative Examples 1 and 7, the E theory of Comparative Example 9 is calculated from the data of Comparative Examples 2 and 7, and the E theory of Comparative Example 10 is calculated from the data of Comparative Examples 4 and 5.

[0062] b) The experimental results show that the actual collagen I enhancement rate after administration of the combination of mulberry root extract and recombinant human collagen III is denoted as Eactual.

[0063] c) Compare E theory and E practice and make a collaborative judgment:

[0064] If Eactual>Etheory, it is efficiency enhancement;

[0065] If Eactual < Etheoretical, it is antagonistic;

[0066] If Eactual = Etheoretical, it is an addition.

[0067] The test results and synergistic judgments of Examples 1 to 5 and Comparative Examples 1 to 10 are shown in Table 2:

[0068] Table 2 Sample concentrations of Examples 1 to 5 and Comparative Examples 1 to 10

[0069]

[0070] As can be seen from Table 2, in Example 1, the Etheoretical of 10 μg / mL mulberry root extract and 5 μg / mL recombinant human collagen III is 28.53%, and the Eactual of Example 1 is 81.20%, and Eactual>Etheoretical, which proves that the mulberry root extract and recombinant human collagen III have a synergistic effect in promoting the synthesis of collagen I in fibroblasts at a ratio of 2:1.

[0071] Similarly, the actual E of Examples 2 to 5 are all greater than the theoretical E, indicating that when the mass ratio of mulberry root extract to recombinant human collagen III is in the range of (2 to 20):1, mulberry root extract and recombinant human collagen III have a synergistic effect in promoting the synthesis of collagen I in fibroblasts.

[0072] Among them, the actual E of Examples 1 to 3 is not only greater than the theoretical E, but the ratio of the actual E to the theoretical E is more than 2.5 times, indicating that when the mass ratio of the mulberry root extract to the recombinant human collagen III is in the range of (2 to 4): 1, the synergistic effect of the mulberry root extract and the recombinant human collagen III in promoting the synthesis of collagen I in fibroblasts is particularly significant.

[0073] In contrast, the actual E of comparative examples 8 to 10 is less than or slightly less than the theoretical E, indicating that within the ratio range of comparative examples 8 to 10, mulberry root extract and recombinant human collagen III do not have a synergistic effect in promoting the synthesis of collagen I in fibroblasts.

[0074] Those skilled in the art will appreciate that the above-mentioned embodiments are specific examples for implementing the present invention, and in actual applications, various changes may be made thereto in form and detail without departing from the spirit and scope of the present invention.

Claims

1. A composition with anti-wrinkle effect, characterized in that: It comprises mulberry root extract and recombinant human collagen III, wherein the mass ratio of the mulberry root extract to the recombinant human collagen III is (2-20):

1.

2. The composition with anti-wrinkle effect according to claim 1, characterized in that: The mass ratio of the mulberry root extract to the recombinant human collagen III is (2-4):

1.

3. The composition with anti-wrinkle effect according to claim 1 or 2, characterized in that: The mulberry root extract is prepared by a method comprising the following steps: (1) taking the root of white mulberry or black mulberry plant, crushing and drying to obtain an extraction raw material; (2) immersing the extraction raw material in a solvent at a ratio of 0.05 kg / L to 0.1 kg / L, and leaching for 36 to 60 hours under stirring to obtain an extract; the solvent is selected from ethanol, propylene glycol, butylene glycol or aqueous solutions of the above solvents; (3) filtering and decolorizing the extract to obtain a mulberry root extract.

4. The anti-wrinkle composition according to claim 3, characterized in that: In the method for preparing the mulberry root extract, the solvent is an aqueous solution of 1,3-propylene glycol with a volume percentage concentration of 70 to 90%.

5. The composition with anti-wrinkle effect according to claim 1 or 2, characterized in that: The recombinant human collagen III is composed of a short amino acid sequence from natural human collagen III as a basic repeating unit, which is repeated multiple times; The basic repeating unit is gergapgfrgpagpngipgekgpagergap, and the number of repetitions of the basic repeating unit is 1 to 16.

6. The anti-wrinkle composition according to claim 5, characterized in that: The molecular weight of the recombinant human collagen III is 3-50KD.

7. Use of the anti-wrinkle composition according to any one of claims 1 to 6 in the preparation of cosmetics.

8. The use according to claim 7, characterized in that The composition having anti-wrinkle efficacy synergistically increases the synthesis of collagen I by fibroblasts.

9. A cosmetic, characterized in that: A composition with anti-wrinkle effect comprising any one of claims 1 to 6.

10. The cosmetic according to claim 9, characterized in that: The mass percentage concentration of the mulberry root extract in the cosmetic is 0.001% to 1%, and the mass percentage concentration of the recombinant human collagen III in the cosmetic is 0.0001% to 1%.

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