Real-time monitoring system for organoid cell culture system environment

Abstract

The invention discloses a real-time monitoring system for an organ-like cell culture system environment. The real-time monitoring system comprises a shell, the microcontroller is arranged in the shell; a culture bottle; the temperature sensor, the oxygen sensor and the pH value sensor are positioned in the culture bottle; the temperature sensor, the oxygen sensor and the pH value sensor are respectively connected with the microcontroller through connecting pipes and are used for monitoring information in the culture bottle. When the cell culture device is used, the cell culture environment is comprehensively and accurately monitored, and it is ensured that cells grow in the safe environment. The pocket-size machine is small in size, convenient to carry and operate and suitable for various cell culture applications in laboratories.

Description

Technical Field

[0001] The present invention relates to the field of cell detection, in particular to a real-time monitoring system for a cell culture system environment, and in particular to a real-time monitoring system for an organoid cell culture system environment. Background Art

[0002] One of the key advances in stem cell research is the development of organoid systems. These in vitro culture systems consist of a self-renewing stem cell population that can differentiate into multiple organ-specific cell types, sharing a similar spatial organization with the corresponding organ and reproducing some of its functions, thus providing a highly physiologically relevant system. Organoids can be generated from tissue samples containing adult stem cells, single adult stem cells, or through the directed differentiation of pluripotent stem cells.

[0003] Organoid cell culture is a fundamental experimental technique in biomedicine, pharmaceutical research, and other fields. Cell growth and differentiation depend on favorable environmental conditions in the culture medium. Temperature, oxygen concentration, and pH are key factors influencing cell growth.

[0004] Traditional cell culture monitoring methods typically require multiple devices to monitor these parameters separately, and acquiring and recording real-time data often relies on complex experimental designs. While some integrated monitoring systems exist, most suffer from complex operation, bulky equipment, and difficulty accessing sensors.

[0005] To this end, we proposed a real-time monitoring system for the organoid cell culture system environment and its preparation method to solve the above problems. Summary of the Invention

[0006] Technical problems to be solved

[0007] In response to the problems existing in the monitoring of organoid cell culture systems in the prior art, the purpose of the present invention is to provide a real-time monitoring system for the cell culture system environment, which solves the problems of complex operation, bulky equipment, and inconvenient portability of the monitoring system.

[0008] Technical Solution

[0009] To solve the above problems, the present invention adopts the following technical solutions.

[0010] A real-time monitoring system for an organoid cell culture system environment comprises a housing;

[0011] a microcontroller mounted in the housing;

[0012] culture flasks;

[0013] Temperature sensor, oxygen sensor and pH sensor, located in the culture bottle;

[0014] The temperature sensor, oxygen sensor and pH sensor are respectively connected to the microcontroller through connecting tubes to monitor the information in the culture bottle;

[0015] Magnetic sheet, located at the bottom of the culture bottle;

[0016] The electromagnet matrix is ​​located at the top of the inner portion of the shell and is configured to attract the magnetic sheet when the electromagnet matrix is ​​energized.

[0017] Preferably, the organoid cells are stem cells, such as embryonic stem cells, mesenchymal stem cells, or Muse cells, more preferably human stem cells.

[0018] For the sake of convenience, "organoid cells" are sometimes referred to as "cells" in this article.

[0019] Correspondingly, the culture medium used in the organoid cell culture system is stem cell culture medium (NEM) such as DMEM / F12 basal medium, neural induction medium (NIM) such as neural induction medium (NIM), differentiation medium such as neural differentiation medium (NDM), etc.

[0020] Correspondingly, the objects monitored by the temperature sensor, oxygen sensor, pH sensor, etc. are the corresponding values ​​of the stem cell culture medium (NEM) such as DMEM / F12 basal culture medium, neural induction medium (NIM) such as neural induction medium (NIM), differentiation medium such as neural differentiation medium (NDM), etc. located in the culture bottle.

[0021] In a new embodiment, the side wall of the culture bottle is provided with three interfaces, the three interfaces are respectively connected to three connecting pipes, and the three interfaces are respectively connected to a temperature sensor, an oxygen sensor and a pH sensor.

[0022] In a new embodiment, it further includes a display screen and buttons, both of which are connected to the housing, and the display screen and buttons are electrically connected to the microcontroller.

[0023] In a new embodiment, it also includes:

[0024] A mounting portion, located in the housing;

[0025] a driving portion, mounted in the mounting portion;

[0026] a movable frame, slidably disposed in the mounting portion and configured to be driven to move by the driving portion;

[0027] A movable rod is rotatably connected to the side wall of the movable frame;

[0028] A connecting rod, rotatably connected to the movable rod;

[0029] an opening, which is formed at the top of the housing and communicates with the mounting portion;

[0030] A folding rod, wherein the middle portion of the folding rod is rotatably connected to the side wall of the opening, one end of the folding rod is rotatably connected to the connecting rod, and the folding rod is configured to contact the side wall of the culture bottle when rotating.

[0031] In a new embodiment, the driving unit includes:

[0032] a drive motor, located in the mounting portion;

[0033] A driving gear, mounted on the driving shaft of the driving motor;

[0034] A rack is mounted on the movable frame and is adapted to the driving gear.

[0035] In a new embodiment, it also includes:

[0036] Power supply department;

[0037] Two connecting wires are respectively connected to the positive and negative electrodes of the power supply unit, and the two connecting wires extend into the mounting portion;

[0038] Two first connectors are connected to the connecting wires respectively, and the two first connectors are connected to the movable frame;

[0039] Two second connectors, adapted to the first connector;

[0040] A wire is connected to the second connector and to the electromagnet matrix.

[0041] In a new embodiment, it also includes:

[0042] a fixing portion, mounted in the mounting portion;

[0043] An insertion rod passes through the fixing portion, and a second connector is connected to one end of the insertion rod;

[0044] The elastic member has one end connected to the inserting rod and the other end connected to the fixing portion.

[0045] In a new embodiment, it also includes:

[0046] A protrusion mounted on the folding rod;

[0047] The wear-resistant pad is installed on the protruding portion and is provided with a plurality of protrusions.

[0048] In a new embodiment, the electromagnet matrix includes multiple groups of electromagnet assemblies, and the multiple groups of electromagnet assemblies are connected in parallel. The electromagnet assemblies include multiple electromagnet parts, and each group of multiple electromagnet parts is connected in series.

[0049] In a new embodiment, the connecting tube is replaced with a wireless connection.

[0050] Beneficial effects: Compared with the prior art, the advantages of the present invention are:

[0051] The real-time monitoring system for organoid cell culture systems provided by this invention is portable and accurately monitors the cell culture environment, ensuring safe cell growth. The compact device is easy to carry and operate, making it suitable for various laboratory cell culture applications, particularly organoid cell culture. BRIEF DESCRIPTION OF THE DRAWINGS

[0052] Figure 1 is a schematic diagram of embodiment 1 of the present invention;

[0053] Figure 2 Schematic diagram of the internal structure of the housing of the present invention;

[0054] Figure 3 It is a structural schematic diagram of the mounting portion of the present invention;

[0055] Figure 4 Schematic diagram of the connection structure of the first connector and the second connector of the present invention.

[0056] The accompanying drawings are denoted as follows:

[0057] 1. Housing; 2. Microcontroller; 3. Culture bottle; 4. Temperature sensor; 5. Oxygen sensor; 6. pH sensor; 7. Connecting tube; 8. Magnetic sheet; 9. Electromagnetic matrix; 10. Display screen; 11. Button; 12. Mounting part; 13. Driving part; 1301. Driving motor; 1302. Driving gear; 1303. Rack; 14. Movable frame; 15. Movable rod; 16. Connecting rod; 17. Folding rod; 18. Power supply; 19. Connecting line; 20. First connector; 21. Second connector; 22. Wire; 23. Fixing part; 24. Insert rod; 25. Elastic member; 26. Protrusion. DETAILED DESCRIPTION

[0058] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention; it is obvious that the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0059] The embodiments of the present application provide a real-time monitoring system for the environment of an organoid cell (referred to as cells) culture system, thereby solving the problem of a complex monitoring system structure and inconvenience in carrying. When in use, it is possible to conveniently carry equipment such as culture bottles, microcontrollers, and display screens.

[0060] The technical solution in the embodiments of the present application is to solve the above technical problems, and the overall idea is as follows:

[0061] The electromagnet matrix 9 and magnetic sheet 8 can attract the culture bottle 3, making it easy to carry the culture bottle 3, microcontroller 2, display screen 10, and other items. Furthermore, the present invention aims to provide a simple and compact cell culture medium monitoring system. This system comprises a culture bottle 3, three detachable connecting tubes 7, a temperature sensor 4, an oxygen sensor 5, and a pH sensor 6. The data from all sensors is connected to a pocket-sized device via the connecting tubes 7, which displays the culture medium's temperature, oxygen concentration, and pH in real time.

[0062] Culture bottle 3 design: Three connection tube 7 interfaces are reserved at the bottom of the culture bottle 3, which are used to connect the temperature sensor 4, oxygen sensor 5 and pH sensor 6 respectively. The connection tube 7 adopts a detachable design for easy installation and replacement.

[0063] Sensor Settings: Temperature Sensor 4: Used to monitor the culture medium temperature. A standard temperature sensor (such as the DS18B20) can be used. Oxygen Sensor 5: Used to measure the dissolved oxygen concentration in the culture medium. Choose an oxygen sensor suitable for cell culture environments (such as the MG-811). pH Sensor 6: Used to monitor the pH of the culture medium in real time. Choose a standard pH sensor (such as the Gravity pH sensor).

[0064] Pocket device: This device includes multiple interfaces that connect to the sensor connectors (7). It reads sensor data wirelessly or wiredly in real time and displays it. The display includes culture medium temperature, oxygen concentration, and pH. The pocket device is compact and suitable for use in a laboratory environment.

[0065] Data processing: The pocket machine integrates a microcontroller 2, which is responsible for collecting sensor data and displaying it in real time on the display 10. Users can operate the system through buttons 11 or the touch screen, including calibration, data storage, and other functions.

[0066] Detachable design: The connecting tube 7 is designed to be detachable, which is convenient for cleaning and replacing the sensor, ensuring the long-term stable use of the system.

[0067] Real-time monitoring: The system can monitor and display key environmental parameters of the culture medium in real time to ensure that cells grow under optimal conditions.

[0068] Easy to operate: The system adopts a detachable connecting tube 7 design, which is convenient for sensor replacement and cleaning, and can adapt to different experimental needs.

[0069] Pocket-sized design: The pocket-sized machine is compact, easy to carry and operate, and suitable for various cell culture applications in the laboratory.

[0070] Automatic data recording: The system can automatically record monitoring data to facilitate later analysis and research.

[0071] Example 1

[0072] See also Figure 1-Figure 4 A real-time monitoring system for an organoid cell culture system environment includes a housing 1; a microcontroller 2 installed in the housing 1; a culture bottle 3; a temperature sensor 4, an oxygen sensor 5, and a pH sensor 6, located in the culture bottle 3; and the temperature sensor 4, the oxygen sensor 5, and the pH sensor 6 are respectively connected to the microcontroller 2 through a connecting tube 7 for monitoring information in the culture bottle 3; a magnetic sheet 8, located at the bottom of the culture bottle 3; an electromagnet matrix 9, located at the top inner side of the housing 1, and configured to attract the magnetic sheet 8 when the electromagnet matrix 9 is energized.

[0073] Preferably, the organoid cells are stem cells, such as embryonic stem cells, mesenchymal stem cells, or Muse cells, more preferably human stem cells.

[0074] Correspondingly, the culture medium used in the organoid cell culture system is stem cell culture medium (NEM) such as DMEM / F12 basal medium, neural induction medium (NIM) such as neural induction medium (NIM), differentiation medium such as neural differentiation medium (NDM), etc.

[0075] Correspondingly, the objects monitored by the temperature sensor 4, oxygen sensor 5 and pH sensor 6 are the corresponding values ​​of the stem cell culture medium (NEM) such as DMEM / F12 basal culture medium, neural induction medium (NIM) such as neural induction medium (NIM), differentiation medium such as neural differentiation medium (NDM), etc. located in the culture bottle.

[0076] Through the cooperation of the electromagnet matrix 9 and the magnetic sheet 8, the culture bottle 3 can be conveniently placed on the housing 1, and the controller, sensor, etc. can be stored in the housing 1 so that they can be combined and carried.

[0077] The microcontroller 2 can be a pocket device. The pocket device integrates the microcontroller 2 and is responsible for collecting sensor data and displaying it in real time on the display screen 10. Users can operate the system through buttons 11 or a touch screen, including calibration, data storage, and other functions.

[0078] The side wall of the culture bottle 3 is provided with three interfaces, which are respectively connected to three connecting pipes 7 , and the three interfaces are respectively connected to a temperature sensor 4 , an oxygen sensor 5 and a pH sensor 6 .

[0079] The device further includes a display screen 10 and buttons 11, both of which are connected to the housing 1 and electrically connected to the microcontroller 2. The display screen 10 clearly displays information monitored and analyzed by the microcontroller 2 and various sensors. The buttons 11 can be operated and are also electrically connected to the drive motor 1301.

[0080] It also includes: a mounting portion 12, located in the shell 1; a driving portion 13, installed in the mounting portion 12; a movable frame 14, slidably arranged in the mounting portion 12, and configured to be driven by the driving portion 13 to move the movable frame 14; a movable rod 15, rotatably connected to the side wall of the movable frame 14; a connecting rod 16, rotatably connected to the movable rod 15; an opening, opened at the top of the shell 1 and connected to the mounting portion 12; a folding rod 17, the middle part of the folding rod 17 is rotatably connected to the side wall of the opening, one end of the folding rod 17 is rotatably connected to the connecting rod 16, and the folding rod 17 is configured to contact the side wall of the culture bottle 3 when rotating.

[0081] After the culture bottle 3 is placed on the shell 1, the driving part 13 can be controlled to drive the movable frame 14 to move, and the movable frame 14 can drive the movable rod 15 and the connecting rod 16 to move. The connecting rod 16 can drive the folding rod 17 to rotate until the side wall of the folding rod 17 contacts the side wall of the culture bottle 3. This can limit the position of the culture bottle 3 and ensure that the culture bottle 3 is sufficiently stable on the shell 1 so that it will not fall when moving.

[0082] The drive unit 13 comprises a drive motor 1301 located in the mounting portion 12; a drive gear 1302 mounted on the drive shaft of the drive motor 1301; and a rack 1303 mounted on the movable frame 14 and mating with the drive gear 1302. The drive motor 1301 stably drives the movable frame 14, and the drive motor 1301 can be a self-locking motor to ensure the position of the folding rod 17. Furthermore, the coordination of the gear and rack 1303 ensures stable control of the position of the rack 1303, the movable frame 14, the connecting rod 16, and the movable rod 15, while preventing mutual interference, ensuring smooth rotation of the folding rod 17.

[0083] It also includes: a power supply unit 18; two connecting wires 19, which are respectively connected to the positive and negative poles of the power supply unit 18, and the two connecting wires 19 extend into the mounting portion 12; two first connecting heads 20, which are respectively connected to the connecting wires 19, and the two first connecting heads 20 are connected to the movable frame 14; two second connecting heads 21, which are adapted to the first connecting heads 20; a wire 22, which is connected to the second connecting head 21 and is connected to the electromagnet matrix 9.

[0084] When the movable frame 14 moves, it brings the first connector 20 and the second connector 21 into contact, thereby powering the electromagnet matrix 9. Once energized, the electromagnets attract the magnetic sheet 8. To remove the culture bottle 3, the drive motor 1301 is reversed, separating the first connector 20 and the second connector 21. This de-energizes the electromagnets, de-magnetizing them and ensuring smooth and easy removal of the culture bottle 3.

[0085] It also includes: a fixing portion 23 installed in the mounting portion 12; an insertion rod 24 passing through the fixing portion 23, and the second connector 21 is connected to one end of the insertion rod 24; an elastic member 25, one end of which is connected to the insertion rod 24 and the other end is connected to the fixing portion 23.

[0086] When the first connector 20 and the second connector 21 come into contact, the rod 24 can be pushed to move, causing the elastic member 25 to deform. This arrangement ensures that the positions of the first connector 20 and the second connector 21 when in contact can be arbitrarily configured, and also ensures the safety of the first connector 20 and the second connector 21 when in contact.

[0087] The utility model further comprises: a protrusion 26 mounted on the folding rod 17; and a wear-resistant pad mounted on the protrusion 26, wherein the wear-resistant pad is provided with a plurality of protrusions.

[0088] The protrusion 26 and the projection can further improve the stability and safety of the protrusion 26 and the projection when they come into contact with each other.

[0089] The electromagnet matrix 9 includes multiple groups of electromagnet assemblies, which are connected in parallel. The electromagnet assemblies include multiple electromagnet parts, and each group of multiple electromagnet parts is connected in series.

[0090] A standard cell culture flask 3 (e.g., 100 mL) has three pre-designed connection tubes 7 at the bottom. Each connection is connected to a corresponding sensor: a temperature sensor 4, an oxygen sensor 5, and a pH sensor 6. Each sensor is connected to a pocket-sized device via a connection tube 7, and the device's display 10 displays real-time data.

[0091] When the sensor needs to be replaced or cleaned, the user can easily remove the sensor connecting tube 7 to perform the corresponding maintenance operations. The system supports wireless data transmission, and the user can synchronize the data to a computer or mobile phone via Bluetooth for easy recording and analysis.

[0092] For culture bottles 3 of different sizes, the system can customize the length and interface type of the connecting tube 7 as needed to adapt to different experimental requirements.

[0093] Example 2

[0094] The connecting tube 7 is replaced by a wireless connection. The wireless connection can ensure the stability of information transmission and also reduce the tediousness of carrying the connecting tube 7.

[0095] The above description is merely a preferred embodiment of the present invention; however, the scope of protection of the present invention is not limited thereto. Any person skilled in the art who, within the technical scope disclosed by the present invention, makes equivalent substitutions or modifications based on the technical solutions and improved concepts of the present invention shall be covered by the scope of protection of the present invention.

[0096] It should be noted that, in this document, relational terms such as first and second, etc., are used only to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply the existence of any such actual relationship or order between these entities or operations. Moreover, the terms "comprises," "comprising," or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article, or device comprising a series of elements includes not only those elements, but also other elements not explicitly listed, or elements inherent to such process, method, article, or device. In the absence of further limitations, an element defined by the phrase "comprising a ..." does not exclude the presence of other identical elements in the process, method, article, or device comprising the element.

[0097] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit the same. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the various embodiments of the present invention.

Claims

1. A real-time monitoring system for an organoid cell culture system environment, characterized in that: include: Housing (1); A microcontroller (2) installed in the housing (1); Culture flask (3); A temperature sensor (4), an oxygen sensor (5) and a pH sensor (6) are located in the culture bottle (3); The temperature sensor (4), the oxygen sensor (5) and the pH sensor (6) are respectively connected to the microcontroller (2) via connecting tubes (7) to monitor the information in the culture bottle (3); A magnetic sheet (8) is located at the bottom of the culture bottle (3); The electromagnet matrix (9) is located at the top of the inner part of the housing (1) and is configured to attract the magnetic attraction sheet (8) when the electromagnet matrix (9) is energized.

2. The real-time monitoring system according to claim 1, characterized in that: The side wall of the culture bottle (3) is provided with three interfaces, the three interfaces are respectively connected to three connecting pipes (7), and the three interfaces are respectively connected to a temperature sensor (4), an oxygen sensor (5) and a pH value sensor (6).

3. The real-time monitoring system according to claim 1, characterized in that: It also comprises a display screen (10) and a button (11), wherein the display screen (10) and the button (11) are both connected to the housing (1), and the display screen (10) and the button (11) are electrically connected to the microcontroller (2).

4. The real-time monitoring system according to claim 1, characterized in that: Also includes: A mounting portion (12) located in the housing (1); A driving part (13) installed in the installation part (12); A movable frame (14) is slidably disposed in the mounting portion (12) and is configured such that the movable frame (14) is driven to move by the driving portion (13); A movable rod (15) is rotatably connected to a side wall of the movable frame (14); A connecting rod (16) rotatably connected to the movable rod (15); An opening is formed at the top of the housing (1) and is in communication with the mounting portion (12); A folding rod (17), wherein the middle portion of the folding rod (17) is rotatably connected to the side wall of the opening, one end of the folding rod (17) is rotatably connected to the connecting rod (16), and the folding rod (17) is configured to contact the side wall of the culture bottle (3) when rotating.

5. The real-time monitoring system according to claim 4, characterized in that: The driving unit (13) comprises: A driving motor (1301) is located in the mounting portion (12); A driving gear (1302) mounted on a driving shaft of the driving motor (1301); The rack (1303) is mounted on the movable frame (14) and is adapted to the driving gear (1302).

6. The real-time monitoring system according to claim 5, characterized in that: Also includes: Power supply unit (18); Two connecting wires (19) are respectively connected to the positive and negative electrodes of the power supply unit (18), and the two connecting wires (19) extend into the mounting unit (12); Two first connectors (20) are respectively connected to the connecting wire (19), and the two first connectors (20) are connected to the movable frame (14); Two second connectors (21), adapted to fit the first connector (20); A conducting wire (22) is connected to the second connector (21) and to the electromagnet matrix (9).

7. The real-time monitoring system according to claim 6, characterized in that: Also includes: A fixing portion (23) installed in the installation portion (12); An insertion rod (24) passes through the fixing portion (23), and the second connector (21) is connected to one end of the insertion rod (24); The elastic member (25) has one end connected to the insertion rod (24) and the other end connected to the fixing portion (23).

8. The real-time monitoring system according to claim 1, characterized in that: Also includes: A protrusion (26) mounted on the folding rod (17); A wear-resistant pad is mounted on the protruding portion (26), and a plurality of protrusions are arranged on the wear-resistant pad.

9. The real-time monitoring system according to claim 1, characterized in that: The electromagnet matrix (9) comprises a plurality of groups of electromagnet assemblies, wherein the plurality of groups of electromagnet assemblies are connected in parallel, and the electromagnet assemblies comprise a plurality of electromagnet parts, wherein each group of the plurality of electromagnet parts are connected in series.

10. The real-time monitoring system according to claim 1, characterized in that: The connecting tube (7) is replaced with a wireless connection.

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