Liquid chromatograph for detecting ademetionine

Abstract

The invention relates to the technical field of liquid chromatographs, and discloses a liquid chromatograph for ademetionine detection, which comprises a tray, a controller, an on-line degasser, a first pump machine, a second pump machine, a detector, an automatic sampler I, an automatic sampler II and a column oven, and is characterized in that the on-line degasser is arranged on the tray; the tray, the controller, the online degasser, the first pump and the second pump are sequentially distributed from top to bottom, the detector, the first automatic sampler and the second automatic sampler are sequentially distributed from top to bottom, the second pump, the second automatic sampler and the column oven are sequentially distributed from left to right, and four solvent bottles are placed in the tray. The solvent bottle is stabilized by arranging the fixing assembly, the suction filtration assembly adopts a replaceable filter element structure to avoid blockage, a T-shaped block and a tension spring are designed below the sample rack to ensure resetting, a cleaning assembly and an exhaust fan are arranged in the column oven to quickly clean leaked liquid and accelerate drying, and the stability, the maintenance convenience and the working efficiency of the equipment are improved.

Description

Technical Field

[0001] The invention relates to the technical field of liquid chromatographs, in particular to a liquid chromatograph for detecting ademetionine. Background Art

[0002] As an efficient separation and analysis technology, liquid chromatography (HPLC) is widely used in the fields of drug analysis, biochemistry, food testing and environmental monitoring. Its core principle is to efficiently separate the components in a mixture through the interaction between the liquid mobile phase and the stationary phase. HPLC has the advantages of high sensitivity, good resolution, accurate quantitative analysis and a wide range of applications. It is especially suitable for the detection of non-volatile and thermally unstable compounds. In recent years, with the development of technology, HPLC has gradually developed towards high throughput, microanalysis and precise separation of complex samples, providing strong support for the detection of bioactive molecules.

[0003] Adenosylmethionine (S-adenosyl-L-methionine, SAMe) is an important endogenous compound that is widely present in organisms and participates in a variety of biochemical reactions, including methylation, transsulfurization, and polyamine synthesis. Due to its importance in the fields of medicine and health products, especially in anti-depression, liver disease treatment, and adjuvant treatment of arthritis, the quality control and quantitative analysis of adenosylmethionine are particularly important. However, the structure of adenosylmethionine is unstable and susceptible to oxidative degradation, so high-performance liquid chromatography technology is needed to achieve its highly sensitive and highly selective analytical detection. A liquid chromatograph specially designed for the detection of adenosylmethionine must have a precise temperature control system, an efficient separation column, and an adapted detector to ensure efficient separation and accurate quantification of adenosylmethionine in a complex matrix.

[0004] A liquid chromatograph for the detection of adenosylmethionine is mainly composed of a mobile phase storage and delivery system, a sample injection system, a separation column system, a detector and a data processing system. Its working principle is to inject the sample into the system through an automatic sample injector, and the mobile phase (such as water-acetonitrile or acid-containing buffer) enters the separation column under the push of a high-pressure pump, and adenosylmethionine and its related components are separated in the separation column according to the difference in affinity with the stationary phase. Subsequently, the separated components enter the detector (such as an ultraviolet detector UV or a mass spectrometer MS), and are detected and quantified by the absorption characteristics or mass spectrum characteristics of adenosylmethionine at a specific wavelength. The entire system controls the temperature through a column oven to ensure the separation effect and sample stability, and finally the data processing system records and analyzes the results to achieve high-sensitivity and high-precision adenosylmethionine detection.

[0005] However, although the existing liquid chromatograph has played an important role in the detection of adenosylmethionine, there are still some areas that need to be improved in actual use. For example, the solution bottle is prone to instability when placed on the tray, which may cause shaking or tipping during operation; the traditional filter head structure is prone to clogging after long-term use, and cleaning and replacement are not convenient, affecting work efficiency; the sample rack of the automatic sampler may cause the device to report an error due to position offset when pushed back, reducing the continuity of operation; at the same time, the cleaning operation caused by solution leakage in the column oven is relatively cumbersome, and there is a lack of auxiliary design for quick drying. Summary of the invention

[0006] In view of the deficiencies in the prior art, the present invention provides a liquid chromatograph for detecting adenosylmethionine, which solves the problems of unstable fixation of solution bottles, difficult maintenance of filter head blockage, sample rack resetting deviation and inconvenient cleaning of column oven during equipment detection.

[0007] To achieve the above purpose, the present invention is implemented by the following technical scheme: a liquid chromatograph for detecting adenosylmethionine, comprising a tray, a controller, an online degasser, a first pump, a second pump, a detector, an autosampler 1, an autosampler 2 and a column oven, characterized in that the tray, the controller, the online degasser, the first pump and the second pump are arranged in sequence from top to bottom, and the detector, the autosampler 1 and the autosampler 2 are arranged in sequence from top to bottom, the second pump, the autosampler 2 and the column oven are arranged in sequence from left to right, four solvent bottles are placed inside the tray, and a fixing component is provided on the outside of the solvent bottle to fix the solvent bottle, a suction filter component is provided inside the solvent bottle to filter the extracted solution, a limiting mechanism is provided inside the autosampler 2 to fix the sample, and a cleaning component is provided at the inner bottom of the column oven to clean the leaked liquid.

[0008] Preferably, the fixing assembly includes a partition, which divides the interior of the tray into two parts evenly, and two fixing blocks 1 are fixedly connected on both sides of the partition, and the side of the fixing block 1 away from the partition is recessed in an arc shape, and a fixing block 2 is correspondingly provided on the side of the recessed fixing block 1, and the solvent bottle is located between the fixing block 1 and the fixing block 2, and two limiting columns are fixedly connected to the side of the fixing block 2 away from the fixing block 1, and the two limiting columns are slidably connected to the side wall of the tray, a pull handle 1 is provided between the two limiting columns away from the side of the fixing block 2, and two force springs are provided between the side of the fixing block 2 away from the solvent bottle and the inner wall of the tray.

[0009] Preferably, a bottle cap is installed on the top of the solvent bottle, and a hose passes through the bottle cap and penetrates into the interior of the solvent bottle and is connected to the suction filter assembly, the suction filter assembly includes an upper suction filter head and a filter cartridge, connecting clips are installed on both sides of the filter cartridge, filter holes are provided inside the filter cartridge, filter elements are distributed inside the filter holes, and card slots are opened on both sides of the upper suction filter head.

[0010] Preferably, the connecting clip strip is deformable under force, and the top bending portion of the connecting clip strip is inserted into the interior of the card slot.

[0011] Preferably, operation panel one, operation panel two, operation panel three and operation panel four are installed on one side of the front end of the first pump, the second pump, the detector and the automatic sampler one, respectively, exhaust valve knobs are arranged on the other side of the front end of the first pump and the second pump, and a mixer is arranged between the right sides of the first pump and the second pump.

[0012] Preferably, a fixing ring is installed on the upper inner side of the second automatic sampler, a radiator is provided on the lower inner side of the second automatic sampler, a limiting mechanism is provided on the inner side of the fixing ring, a bottom pad is installed on the inner bottom side of the limiting mechanism, side pads are installed on both inner sides of the limiting mechanism, a limiting component is provided on the top of the bottom pad, the limiting component 1 includes a sample rack, the sample rack slides on the top of the bottom pad, a T-block 1 is fixedly connected to the bottom rear side of the sample rack, a T-slot 1 is provided inside the bottom pad, the T-block 1 is slidably connected to the inside of the T-slot 1, and a tension spring is installed between the T-block 1 and the inner wall of the second automatic sampler, a plurality of sample fixing holes are provided inside the sample rack, a connecting plate is fixedly connected to the front side of the sample rack, and a handle 2 is provided on the top of the connecting plate.

[0013] Preferably, an auxiliary sample assembly is arranged on the side of the side pad, and the auxiliary sample assembly includes a No. 0 frame, a plurality of fixing holes are opened inside the No. 0 frame, a hand-held block is arranged on the front side of the No. 0 frame, a side of the No. 0 frame close to the side pad is fixedly connected with a T-block 2, a T-slot 2 is opened inside the side pad, and the T-block 2 is slidably connected inside the T-slot 2.

[0014] Preferably, an operation panel 5 is installed at the front end of the column temperature box, and a liquid leakage sensor is installed on the inner wall of the column temperature box.

[0015] Preferably, two clamping blocks are fixedly connected to the inner rear side wall of the column oven, and a C18 chromatographic column is clamped between the two clamping blocks. An injection tube is provided on the side wall of the column oven, and the injection tube is connected to the C18 chromatographic column.

[0016] Preferably, a cleaning assembly is provided at the inner bottom of the column temperature box, and the cleaning assembly includes a cleaning tray and an exhaust fan. A handle three is provided at the front end of the cleaning tray. The cleaning tray is slidably connected to the inner bottom wall of the column temperature box, and the exhaust fan is located on the side wall of the column temperature box.

[0017] The present invention provides a liquid chromatograph for detecting adenosylmethionine, which has the following beneficial effects:

[0018] 1. The present invention provides a fixing assembly on the tray, divides the tray into two parts by a partition, and fixes two fixing blocks 1 on both sides of the partition, and provides a fixing block 2 on the side opposite to the fixing block 1, and fixes two limiting columns on the fixing block 2 so that the fixing block 2 can slide on the side wall of the tray, and is connected to the fixing block 2 by a spring, so that the fixing block 2 can be pressed against the outside of the solvent bottle under the action of the spring, and clamp the solution bottle together with the fixing block.

[0019] 2. In the present invention, a suction filter assembly is arranged inside the solution bottle. Compared with the traditional filter head, the interior will inevitably be clogged after working for a long time, resulting in reduced working efficiency. Therefore, the suction filter head is divided into two parts, the lower part is a filter cartridge, and a filter element is installed in the filter cartridge to filter the solution. Connecting strips are installed on both sides of the filter cartridge, which can be upwardly clamped in the outer card slot of the upper filter head through the connecting strips. When the filter element needs to be replaced, it only needs to turn the connecting strip, which is very convenient.

[0020] 3. In the present invention, a T-block is arranged under the sample rack, and a corresponding T-slot is provided on the bottom pad under the sample rack. The T-block is connected to the inner wall of the automatic sampler by a tension spring, so that after the sample rack is pulled out to take out or place the sample, the sample rack will return to its original position under the action of the tension spring, and the operation of the equipment will not be delayed.

[0021] 4. In the present invention, a cleaning component is provided in the column temperature box. During installation, leaked liquid will fall into the cleaning tray. When cleaning is required, the cleaning tray can be pulled out for quick cleaning. After the detection is completed, the exhaust fan is started to accelerate the rapid drying of the inside of the column temperature box. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 A perspective view of the present invention;

[0023] Figure 2 It is a structural schematic diagram of a fixing block 1 of the present invention;

[0024] Figure 3 It is a structural schematic diagram of the suction filter assembly of the present invention;

[0025] Figure 4 It is a structural schematic diagram of the connection card strip of the present invention;

[0026] Figure 5 It is a structural schematic diagram of the limiting mechanism of the present invention;

[0027] Figure 6 It is a schematic diagram of the structure of the sample holder of the present invention;

[0028] Figure 7 It is a structural schematic diagram of the zero frame of the present invention;

[0029] Figure 8 It is a structural schematic diagram of the liquid leakage sensor of the present invention;

[0030] Fig. 9 It is a schematic structural diagram of the cleaning disc of the present invention.

[0031] Among them, 1. tray; 2. controller; 3. online degasser; 4. first pump; 5. operation panel 1; 6. second pump; 7. operation panel 2; 8. exhaust valve knob; 9. mixer; 10. operation panel 3; 11. operation panel 4; 12. detector; 13. autosampler 1; 14. autosampler 2; 15. column oven; 16. operation panel 5; 17. solvent bottle; 18. bottle cap; 19. fixing assembly; 191. partition; 192. fixing block 1; 193. fixing block 2; 194. limiting column; 195. force spring; 196. pull handle 1; 20. suction filter assembly; 201. upper suction filter head; 202. slot; 203. filter element; 2 04, filter hole; 205, filter cartridge; 206, connecting card strip; 21, fixing ring; 22, limiting mechanism; 221, sample rack; 222, sample fixing hole; 223, connecting plate; 224, pull handle two; 225, T-block one; 226, tension spring; 227, T-slot one; 228, auxiliary sample assembly; 2281, zero rack; 2282, handheld block; 2283, T-block two; 2284, T-slot two; 23, bottom pad; 24, side pad; 25, card block; 26, C18 chromatographic column; 27, injection tube; 28, leakage sensor; 29, cleaning assembly; 291, cleaning tray; 292, pull handle three; 293, exhaust fan; 30, radiator. DETAILED DESCRIPTION

[0032] The following will be combined with the drawings in the specification of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0033] Please refer to the attached Figure 1 -Attached Figure 2The embodiment of the present invention provides a liquid chromatograph for detecting adenosylmethionine, including a tray 1, a controller 2, an online degasser 3, a first pump 4, a second pump 6, a detector 12, an autosampler 13, an autosampler 2 14 and a column oven 15. The tray 1, the controller 2, the online degasser 3, the first pump 4 and the second pump 6 are arranged in sequence from top to bottom to form a compact and orderly structural layout to reduce the space occupied by the equipment and optimize the operation process. The detector 12, the autosampler 13 and the autosampler 2 14 are also arranged in sequence from top to bottom to ensure efficient and accurate sample transmission during the detection process. At the same time, the second pump 6, the autosampler 2 14 and the column oven 15 are arranged in sequence from left to right to achieve seamless connection of the flow path with reasonable spatial arrangement, which is convenient for maintenance and operation. The front end side of the first pump 4 and the second pump 6 are respectively installed with an operation panel 1 5 and an operation panel 2 7 for adjusting and controlling the operation of the pump. The operation interface design is intuitive and concise, which is convenient for users to monitor and adjust the flow path parameters in real time. The front ends of the detector 12 and the automatic sample injector 13 are respectively provided with an operation panel 3 10 and an operation panel 4 11. The other side of the front end of the first pump 4 and the second pump 6 are provided with an exhaust valve knob 8 for manually or automatically exhausting gas to ensure the stability of the flow system and avoid the interference of bubbles on separation and detection. A mixer 9 is provided between the right side of the first pump 4 and the second pump 6, which can efficiently mix multiple mobile phases to achieve uniformity of mobile phase components, meet the high-precision requirements of liquid phase separation and complex sample detection, and further improve the overall performance and detection reliability of the equipment.

[0034] Four solvent bottles 17 are placed inside the tray 1, and a fixing assembly 19 is provided on the outside of each solvent bottle 17 to firmly fix the solvent bottle 17 to prevent the solvent bottle 17 from shaking or tipping over due to vibration or operation during operation. The structure of the fixing assembly 19 is exquisite and reasonable, including a partition 191, which divides the inside of the tray 1 into two parts evenly, so as to reasonably place the solvent bottle 17. Two fixing blocks 192 are fixedly connected to both sides of the partition 191, and the side of the fixing block 192 away from the partition 191 is designed to be an arc-shaped depression, so as to better fit the outside of the solvent bottle 17. A fixing block 2 193 is correspondingly provided on one side of the depression of the fixing block 192, and the fixing block 2 193 and the fixing block 1 192 are arranged relative to each other to form a structure for clamping the solvent bottle 17. The side of the fixed block 193 away from the fixed block 192 is fixedly connected with two limit posts 194, and the limit posts 194 are slidably connected to the inside of the side wall of the tray 1, so that the fixed block 193 can slide along the side wall to adjust its position, so as to adapt to solvent bottles 17 of different sizes. A pull handle 196 is also provided at the far end between the two limit posts 194, so that the user can adjust the position of the fixed block 193. The side of the fixed block 193 away from the solvent bottle 17 is connected to the inner wall of the tray 1 through two force springs 195. Under the action of the force springs 195, the fixed block 193 always applies pressure to the outside of the solvent bottle 17, and clamps the solvent bottle 17 together with the fixed block 192, so as to achieve a stable fixation and reduce the impact of vibration on the solvent bottle 17, thereby ensuring the stability and safety of the equipment operation.

[0035] Please refer to the attached Figure 3 -Attached Figure 4 , a bottle cap 18 is installed on the top of the solvent bottle 17, the design of the bottle cap 18 ensures sealing, and the hose passes through the bottle cap 18 and penetrates into the interior of the solvent bottle 17, and is connected to the suction filter assembly 20. The suction filter assembly 20 has a reasonable structure and is easy to maintain, including an upper suction filter head 201 and a filter cartridge 205, wherein the filter cartridge 205 is used to filter the solvent. Connecting card strips 206 are installed on both sides of the filter cartridge 205, and a plurality of filter holes 204 are arranged inside the filter cartridge 205, and filter elements 203 are evenly distributed in the filter holes 204, which are used to efficiently intercept particulate matter or impurities in the solvent. Card slots 202 are provided on both sides of the upper suction filter head 201, which are used in conjunction with the connecting card strips 206. The connecting card strip 206 has an elastic design, which can be appropriately deformed after being subjected to force, and the curved part of its top can be firmly plugged into the inside of the card slot 202. With this design, when the filter element 203 needs to be replaced, the filter cartridge 205 can be quickly removed by gently turning the connection clip 206. The operation is simple and convenient, and there is no need to disassemble the entire suction filter assembly 20, which greatly improves the maintenance efficiency and reduces the risk of affecting the operation of the equipment due to the blockage of the filter element 203. Such an integrated and modular design of the suction filter assembly 20 ensures the long-term and efficient operation of the equipment, while facilitating the daily maintenance and operation of the user.

[0036] Please see attached Figure 5 -Attached Figure 7 The internal design of the automatic sampler 214 is scientific and reasonable. A fixing ring 21 is installed on the upper side for fixing key components, and a radiator 30 is arranged on the lower side, which can effectively reduce the heat generated when the equipment is running and keep the internal temperature stable. A limiting mechanism 22 is arranged on the inner side of the fixing ring 21. The structure of the limiting mechanism 22 is complex and precise. A bottom pad 23 is installed on the bottom side of the bottom pad 23. Side pads 24 are installed on both sides of the bottom pad 23 to provide support and guidance functions for the sample rack 221. A limiting assembly is arranged on the top of the bottom pad 23. The limiting assembly includes a sample rack 221 slidably connected to the top of the bottom pad 23. A T-block 225 is fixedly connected to the bottom rear side of the sample rack 221. A T-slot 227 matching it is opened inside the bottom pad 23, and the T-block 225 is slidably connected inside the T-slot 227. The T-block 1 225 is connected to the inner wall of the automatic sampler 2 14 by a tension spring 226, so that the sample rack 221 can automatically return to its original position under the action of the tension spring 226 after being pulled out, avoiding affecting the operation of the equipment due to the incorrect return of the sample rack 221. The sample rack 221 has a plurality of sample fixing holes 222 inside for fixing sample bottles, and a connecting plate 223 is fixedly connected to the front side thereof, and a handle 224 is provided on the top of the connecting plate 223, so that the user can operate the sample rack 221 conveniently. In addition, in order to further improve the convenience of sample operation, an auxiliary sample assembly 228 is also provided on the side of the side pad 24. The auxiliary sample assembly 228 includes a zero rack 2281, and a plurality of fixing holes are provided inside the zero rack 2281 for additional sample storage, and a hand-held block 2282 is designed on the front side thereof for easy operation and placement. A T-block 2283 is fixedly connected to one side of the zero rack 2281 near the side pad 24, and a matching T-slot 2284 is provided inside the side pad 24. The T-block 2283 is slidably connected inside the T-slot 2284, so that the auxiliary sample assembly 228 can be flexibly adjusted and positioned, further enhancing the sample fixation and placement capabilities. The overall design ensures the stable operation of the sample rack 221 and the precise fixation of the sample, while providing more convenience for sample operation through the auxiliary sample assembly 228, greatly improving the functionality and reliability of the automatic sample injector, and meeting the high requirements for sample processing in complex experiments.

[0037] Please see attached Figure 8 -Attached Fig. 9The design of the column oven 15 combines functionality and convenience, and an operation panel 5 16 is installed at its front end, which is convenient for users to intuitively operate and adjust. A leakage sensor 28 is installed on the inner wall of the column oven 15, which can monitor the leakage inside the box in real time. Once an abnormality occurs, an alarm can be issued in time and corresponding measures can be taken to improve the safety and stability of the equipment. Two card blocks 25 are fixedly connected to the inner rear side wall of the column oven 15, and a C18 chromatographic column 26 is clamped between the two card blocks 25. The C18 chromatographic column 26 is firmly installed through this structure to avoid column position displacement or loosening due to vibration during operation, thereby ensuring the stability of the separation effect. An injection pipe 27 is provided on the side wall of the column oven 15, and the injection pipe 27 is connected to the C18 chromatographic column 26 for injecting mobile phase or sample to ensure the smoothness of the flow path and the convenience of operation. In addition, a cleaning component 29 is provided at the inner bottom of the column oven 15, and the cleaning component 29 is composed of a cleaning disk 291 and an exhaust fan 293 to form an integrated cleaning system. A handle 292 is provided at the front end of the cleaning tray 291. The user only needs to gently pull the handle 292 to slide the cleaning tray 291 out from the bottom of the column oven 15, which is used to quickly collect and clean the leakage generated during installation or operation. The cleaning tray 291 is slidably connected to the inner bottom wall of the column oven 15, which is easy to disassemble and simple to maintain, greatly improving the operability of the equipment. In order to further optimize the cleaning efficiency, an exhaust fan 293 is installed on the side wall of the column oven 15. After cleaning is completed, the exhaust fan 293 can be started to quickly dry the interior of the column oven 15 to avoid the adverse effects of liquid residue on equipment performance or subsequent operation. The overall design not only ensures the cleanliness and dryness of the interior of the column oven 15, but also effectively extends the service life of the equipment, while improving the reliability and safety of equipment operation.

[0038] Working principle: The liquid chromatograph for detecting adenosylmethionine of the present invention realizes the full process operation of solvent delivery, sample injection, separation analysis and detection through the synergistic effect of various functional components. First, the four solvent bottles 17 placed in the tray 1 are firmly fixed by the fixing assembly 19 to avoid shaking and tipping, and the adaptability is strong and convenient for placement and removal operations. A suction filter assembly 20 is installed inside the solvent bottle 17. The suction filter assembly 20 includes an upper suction filter head 201 and a filter cartridge 205. A filter element 203 is arranged in the filter cartridge 205 for filtering the solution. The hose passes through the bottle cap 18 to connect the suction filter assembly 20, and the filtered solution is delivered to the first pump 4 and the second pump 6. The pump controls the flow rate through the operation panel 1 5 and the operation panel 2 7, and the mobile phase is mixed through the mixer 9, and finally sent to the automatic sampler 13, and the control operation panel 4 11 is used to process the sample. The sample is loaded into the sample rack 221 of the limiting mechanism 22 through the automatic sample injector 214. The sample rack 221 is provided with a plurality of sample fixing holes 222. The sample rack 221 is automatically reset under the action of the tension spring 226 through the sliding fit of the T-slot 1 227 of the bottom pad 23 and the T-block 1 225, so as to avoid the equipment error caused by the incorrect insertion position of the sample rack 221, thereby affecting the efficiency of the detection. Subsequently, the mixed solution and the sample enter the column oven 15 together. A C18 chromatographic column 26 is fixed in the column oven 15, and the injection tube 27 injects the solution into the chromatographic column for separation and analysis. During the detection process, the cleaning component 29 at the bottom of the column oven 15 collects possible leakage through the cleaning plate 291, the exhaust fan 293 accelerates drying, and the leakage sensor 28 monitors in real time. Finally, the detector 12 receives the signal and outputs the detection result through the controller 2 and the operation panel 3 10, thereby realizing the efficient detection of adenosylmethionine.

[0039] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A liquid chromatograph for detecting adenosylmethionine, comprising a tray (1), a controller (2), an online degasser (3), a first pump (4), a second pump (6), a detector (12), an automatic sample injector 1 (13), an automatic sample injector 2 (14) and a column oven (15), characterized in that: The tray (1), the controller (2), the online degasser (3), the first pump (4) and the second pump (6) are arranged in sequence from top to bottom, and the detector (12), the first autosampler (13) and the second autosampler (14) are arranged in sequence from top to bottom, the second pump (6), the second autosampler (14) and the column oven (15) are arranged in sequence from left to right, four solvent bottles (17) are placed inside the tray (1), and a fixing assembly (19) is arranged on the outside of the solvent bottle (17) for fixing the solvent bottle (17), a suction filter assembly (20) is arranged inside the solvent bottle (17) for filtering the extracted solution, a limiting mechanism (22) is arranged inside the second autosampler (14) for fixing the sample, and a cleaning assembly (29) is arranged at the inner bottom of the column oven (15) for cleaning the leaked liquid.

2. A liquid chromatograph for detecting ademetionine according to claim 1, characterized in that, The fixing assembly (19) comprises a partition (191), wherein the partition (191) divides the interior of the tray (1) into two equal parts, and two fixing blocks (192) are fixedly connected to both sides of the partition (191), and the side of the fixing block (192) away from the partition (191) is concave in an arc shape, and a fixing block (193) is provided corresponding to the concave side of the fixing block (192), and the solvent bottle (17) is located between the fixing block (192) and the fixing block (193). ), the side of the fixed block 2 (193) away from the fixed block 1 (192) is fixedly connected with two limiting columns (194), and the two limiting columns (194) are slidably connected to the inside of the side wall of the tray (1), a pull handle (196) is arranged between the two limiting columns (194) away from the side of the fixed block 2 (193), and two force springs (195) are arranged between the side of the fixed block 2 (193) away from the solvent bottle (17) and the inner wall of the tray (1).

3. A liquid chromatograph for detecting ademetionine according to claim 1, characterized in that, A bottle cap (18) is installed on the top of the solvent bottle (17), and a hose passes through the bottle cap (18) and penetrates into the interior of the solvent bottle (17) and is connected to the suction filter assembly (20). The suction filter assembly (20) comprises an upper suction filter head (201) and a filter cartridge (205). Connecting clamping strips (206) are installed on both sides of the filter cartridge (205). Filter holes (204) are arranged inside the filter cartridge (205). Filter cores (203) are distributed throughout the filter holes (204). Clamping slots (202) are provided on both sides of the upper suction filter head (201).

4. A liquid chromatograph for detecting ademetionine according to claim 3, characterized in that, The connecting clamping strip (206) can be deformed when subjected to force, and the top bending portion of the connecting clamping strip (206) is inserted into the interior of the clamping slot (202).

5. A liquid chromatograph for detecting ademetionine according to claim 1, characterized in that, An operation panel 1 (5), an operation panel 2 (7), an operation panel 3 (10) and an operation panel 4 (11) are respectively installed on one side of the front end of the first pump (4), the second pump (6), the detector (12) and the automatic sample injector 1 (13); an exhaust valve knob (8) is arranged on the other side of the front end of the first pump (4) and the second pump (6); and a mixer (9) is arranged between the right sides of the first pump (4) and the second pump (6).

6. A liquid chromatograph for detecting ademethionine according to claim 1, characterized in that: A fixing ring (21) is installed on the upper inner side of the second automatic sampler (14), a heat sink (30) is arranged on the lower inner side of the second automatic sampler (14), a limiting mechanism (22) is arranged on the inner side of the fixing ring (21), a bottom pad (23) is installed on the inner bottom side of the limiting mechanism (22), side pads (24) are installed on both inner sides of the limiting mechanism (22), a limiting component is arranged on the top of the bottom pad (23), the limiting component 1 comprises a sample rack (221), the sample rack (221) slides on the top of the bottom pad (23), the sample rack A T-block (225) is fixedly connected to the rear side of the bottom of (221), a T-slot (227) is provided inside the bottom pad (23), the T-block (225) is slidably connected inside the T-slot (227), and a tension spring (226) is installed between the T-block (225) and the inner wall of the automatic sampler (14), a plurality of sample fixing holes (222) are provided inside the sample rack (221), a connecting plate (223) is fixedly connected to the front side of the sample rack (221), and a handle (224) is provided on the top of the connecting plate (223).

7. A liquid chromatograph for detecting ademethionine according to claim 6, characterized in that: An auxiliary sample assembly (228) is arranged on the side of the side pad (24), and the auxiliary sample assembly (228) includes a No. 0 frame (2281), a plurality of fixing holes are provided inside the No. 0 frame (2281), a hand-held block (2282) is arranged on the front side of the No. 0 frame (2281), a T-shaped block 2 (2283) is fixedly connected to a side of the No. 0 frame (2281) close to the side pad (24), a T-shaped slot 2 (2284) is provided inside the side pad (24), and the T-shaped block 2 (2283) is slidably connected inside the T-shaped slot 2 (2284).

8. A liquid chromatograph for detecting ademethionine according to claim 1, characterized in that: An operation panel 5 (16) is installed at the front end of the column temperature box (15), and a liquid leakage sensor (28) is installed on the inner wall of the column temperature box (15).

9. A liquid chromatograph for detecting ademethionine according to claim 1, characterized in that: Two clamping blocks (25) are fixedly connected to the inner rear side wall of the column temperature box (15), and a C18 chromatographic column (26) is clamped between the two clamping blocks (25). An injection pipe (27) is arranged on the side wall of the column temperature box (15), and the injection pipe (27) is connected to the C18 chromatographic column (26).

10. A liquid chromatograph for detecting ademethionine according to claim 1, characterized in that: A cleaning assembly (29) is provided at the inner bottom of the column temperature box (15), and the cleaning assembly (29) includes a cleaning tray (291) and an exhaust fan (293). A handle (292) is provided at the front end of the cleaning tray (291). The cleaning tray (291) is slidably connected to the inner bottom wall of the column temperature box (15), and the exhaust fan (293) is located on the side wall of the column temperature box (15).

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