Filtering and diluting equipment for phage culture

Abstract

The invention discloses filtering and diluting equipment for bacteriophage culture and belongs to the technical field of bacteriophage culture. A hollow fixed discharging column is arranged in the middle of a mother liquor bin, a linkage ring is rotatably arranged on the inner wall of a culture bin, and a rotating shaft penetrating through a constant-temperature bin is movably mounted on a rotating seat; a linkage bevel gear meshed with the vertical tooth part is mounted in the middle of the rotating shaft; the tail end of the rotating shaft is connected with a driving swing arm, and a lifting seat is movably connected between the driven swing arms; a diluent bin is arranged on the top of the lifting base in a surrounding mode, and a moving ejector rod matched with the upper ejector plug is fixedly arranged in the middle of the lifting base. A hollow movable discharging column is further arranged on the lifting base, and a fixed ejector rod is arranged at the top of the discharging bin. The lifting seat can drive the dilution cavity to move up and down in a reciprocating mode, and when the lifting seat moves upwards, mother liquor in the mother liquor bin can fall into the dilution cavity on the lower portion. When the lifting seat moves downwards, the diluted bacteriophage can fall into the drawing bin, and the automation degree and the dilution efficiency are improved through the design.

Description

Technical Field

[0001] The invention relates to the technical field of bacteriophage culture, in particular to a filtering and diluting device used for bacteriophage culture. Background Art

[0002] Bacteriophages are viruses that invade bacteria and are also genetic materials that give host bacteria biological traits. Bacteriophages must parasitize in living bacteria and have strict host specificity, which depends on the molecular structure and complementarity of the phage adsorption organs and the receptors on the surface of the receptor bacteria. Bacteriophages are the most common and widely distributed group of viruses. Bacteriophages can usually be found in places full of bacterial communities, such as soil and animal intestines. In terms of sterilization effect, the concentration of phages is also one of the important indicators. Therefore, during the research process, it is also necessary to appropriately dilute the concentration of phage culture solution to observe the sterilization effect of phages at different concentrations. If the concentration of phage culture solution is adjusted by chemical tools such as rubber-tipped droppers, it is necessary to observe the scale by naked eye to judge the volume of the added culture solution, so there may be a large error problem. Therefore, a more accurate dilution device is needed to dilute the phage culture solution.

[0003] The patent application with publication number CN116144486A discloses a bacterial cell filtration diluter and a method of use, which relates to the technical field of phage filtration dilution, in order to solve the problem that the weight of the mother liquid will be reduced during the filtration process after the transfer, resulting in changes in dilution, and dilution according to the original ratio will cause the concentration to be too high, and at the same time, natural mixing is required during the dilution process, which is difficult to adapt to external stirring, and the problem of uneven dilution concentration is prone to occur at this time, including: a dilution box; the front end of the dilution box is hingedly installed with a lower sealing door and an upper sealing door, and the lower sealing door is located below the upper sealing door; the lower end of the rear end side wall of the dilution box is fixedly installed with a dilution liquid storage box; the middle part of the upper end of the dilution liquid storage box is fixedly installed with a metering pump; the upper end of the back side wall of the dilution box is fixedly installed with a control panel. The invention thus achieves the purpose of precise dilution, ensures the accuracy of the dilution concentration of the phage mother liquid, and realizes the effect of accurately controlling the dilution concentration.

[0004] However, the above disclosed bacterial cell filtration diluter has a general degree of automation, low processing efficiency, is time-consuming and labor-intensive to use, and cannot automatically add diluent and dilute the mother liquor. Summary of the invention

[0005] The purpose of the present invention is to provide a filtering and diluting device for phage culture in order to solve the problems that the existing bacterial filter diluters have a general degree of automation, low processing efficiency, are time-consuming and labor-intensive to use, and cannot automatically add diluent and dilute the mother liquor.

[0006] To achieve the above-mentioned purpose, the technical scheme of the present invention is: a filtering and dilution device for phage culture, comprising a mother liquid bin, a culture bin and a feed bin, wherein the mother liquid bin, the culture bin and the feed bin are assembled in sequence from top to bottom; a hollow fixed feed column is fixedly arranged in the middle of the mother liquid bin, a filter hole is opened in the lower half of the fixed feed column, and an upper top plug is movably arranged inside the fixed feed column; a constant temperature bin is arranged in the middle of the culture bin, a linkage ring is rotatably arranged on the inner wall of the culture bin, the linkage ring consists of two parts: a vertical tooth part at the top and a gear ring part at the bottom, and a plurality of sets of rotating seats are arranged on the inner wall of the culture bin, and the rotating seats are movably arranged on the rotating seats A rotating shaft passing through the constant temperature bin is installed, and a linkage bevel gear meshing with the vertical tooth portion is installed in the middle of the rotating shaft; the end of the rotating shaft is connected to an active swing arm located in the constant temperature bin, and the end of the active swing arm is movably connected to a driven swing arm, and a lifting seat is movably connected between the driven swing arms; a diluent bin is arranged around the top of the lifting seat, and a moving push rod cooperating with the upper push plug is fixedly arranged in the middle of the lifting seat; a hollow moving unloading column is also arranged on the lifting seat, and a fixed push rod cooperating with the unloading bin is arranged on the top of the lifting seat, and when the lifting seat reciprocates up and down, the mother liquid can be automatically unloaded and diluted for culture.

[0007] As a further solution of the present invention: a positioning seat cooperating with the mother liquid tank is arranged on the top of the culture tank, an upper threaded portion is opened on the bottom outer wall of the mother liquid tank, and a lower threaded portion is opened on the outer wall of the positioning seat; a threaded ring is also sleeved on the outer wall of the positioning seat, and a plurality of groups of protrusions are arranged on the outer wall of the threaded ring.

[0008] As a further solution of the present invention: the top of the upper top plug is connected to multiple groups of return springs, and the upper top plug is connected to the top of the fixed unloading column through the return spring; the top of the mother liquid bin is installed with a top cover, and the top cover is provided with a feeding port.

[0009] As a further solution of the present invention: a mounting boss is provided on the inner wall of the culture bin, a limiting rail is provided on the inner side of the mounting boss, and a T-shaped slot matching the mounting boss is provided on the outer wall of the linkage ring; a convex plate is also provided on the inner wall of the culture bin, a lifting motor is installed on the convex plate, and a driving gear meshing with the gear ring is installed on the output end of the lifting motor.

[0010] As a further solution of the present invention: a heating chamber is opened inside the inner wall of the constant temperature bin, a plurality of groups of heating tubes are installed in the heating chamber, and a temperature sensor located inside the constant temperature bin is installed on the top of the lower bin.

[0011] As a further solution of the present invention: a dilution chamber for the mother liquor is formed between the dilution liquid bins, a pump is also installed on the dilution liquid bin, an output end of the pump is provided with a discharge pipe facing the dilution chamber, and a liquid filling port is also provided on the top of the dilution liquid bin; a plurality of stirring motors are installed at the bottom of the lifting seat, and a stirring fan located in the dilution chamber is provided at the output end of the stirring motor.

[0012] As a further solution of the present invention: a first liquid level sensor is arranged at the bottom of the mother liquid bin, and a second liquid level sensor is arranged at the bottom of the dilution liquid bin; a plurality of groups of supporting legs are arranged on the outer wall of the culture bin, and a control panel is also arranged on the outer wall of the culture bin.

[0013] As a further solution of the present invention: a first liquid drainage groove is provided in the lower half of the movable material discharge column, and a lower top plug is movably arranged inside the movable material discharge column; the top of the lower top plug is connected to multiple groups of reset springs 2, and the lower top plug is connected to the top of the movable material discharge column through the reset springs 2; the fixed top rod is a hollow structure, and a second liquid drainage groove cooperating with the first liquid drainage groove is provided on the outer wall of the upper half of the fixed top rod.

[0014] As a further solution of the present invention: the lower material bin is internally movablely provided with a drawer bin located below the fixed top rod, and a handle is provided on the outer wall of the drawer bin.

[0015] As a further solution of the present invention: the active swing arm and the driven swing arm located on the corresponding two sides of the lifting seat are symmetrically installed.

[0016] Compared with the prior art, the present invention has the following beneficial effects: The present invention can quickly disassemble and assemble the mother liquid bin, the culture bin and the feed bin through the assembled structure, so as to inspect the interior and add diluent. With the cooperation of the linkage ring and multiple sets of rotating shafts, the lifting seat can drive the dilution chamber to reciprocate up and down in the constant temperature bin. When the lifting seat drives the moving top rod to move upward, the moving top rod will squeeze the upper top plug upward, and at this time the phage mother liquid in the mother liquid bin will fall into the dilution chamber below through the filter hole. Then, driven by the pump, the diluent in the dilution liquid bin will be added to the dilution chamber in proportion. When the lifting seat drives the moving feed column to move downward, the fixed top rod will squeeze the lower top plug upward, and at this time the phage diluted in the dilution chamber will fall into the pull-out bin below through the first liquid discharge trough and the second liquid discharge trough. This design improves the automation and dilution efficiency of the filtering and dilution equipment used for phage culture. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] The present invention will be further explained below in conjunction with the accompanying drawings and embodiments: Figure 1 It is a three-dimensional structural diagram of the present invention; Figure 2 is a cross-sectional view of the present invention; Figure 3 is a cross-sectional view of the culture chamber of the present invention; Figure 4 yes Figure 3 A magnified view of the structure at center A; Figure 5 It is a three-dimensional structural diagram of the culture chamber in the present invention; Figure 6 It is a three-dimensional structural diagram of the linkage ring and the lifting seat in the present invention; Figure 7 It is a cross-sectional view of a lifting seat in the present invention.

[0018] Description of reference numerals: 1. Mother liquid bin; 101. Fixed feed column; 102. Filter hole; 103. Top plug; 104. Reset spring 1; 105. First liquid level sensor; 106. Top cover; 107. Feeding port; 108. Upper threaded portion; 2. Cultivation chamber; 201. Support legs; 202. Control panel; 203. Positioning seat; 204. Lower threaded portion; 205. Threaded ring; 206. Raised portion; 207. Constant temperature chamber; 208. Heating chamber; 209. Heating tube; 210. Mounting boss; 211. Limit rail; 212. Linkage ring; 213. Vertical tooth portion; 214. Gear ring portion; 215. T-slot; 216. Rotating seat; 217. Rotating shaft; 218. Linkage bevel gear; 219. Active swing arm; 220, driven swing arm; 221, lifting seat; 222, diluent bin; 223, diluent chamber; 224, moving ejector rod; 225, pump; 226, liquid discharge pipe; 227, liquid filling port; 228, second liquid level sensor; 229, stirring motor; 230, stirring fan; 231, moving material discharge column; 232, first liquid discharge tank; 233, lower ejector plug; 234, second return spring; 235, convex plate; 236, lifting motor; 237, driving gear; 3. Unloading bin; 301. Fixed ejector rod; 302. Second row of liquid tanks; 303. Temperature sensor; 304. Pull-out bin; 305. Handle. DETAILED DESCRIPTION

[0019] The following will be combined with the attached Figures 1 to 7 The technical solution of the present invention is described clearly and completely. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0020] The present invention provides a filter dilution device for phage culture by improvement, such as Figure 1-Figure 7 As shown, it includes a mother liquid bin 1, a culture bin 2 and a feed bin 3, which are assembled in sequence from top to bottom; a hollow fixed feed bin 101 is fixedly arranged in the middle of the mother liquid bin 1, a filter hole 102 is opened in the lower half of the fixed feed bin 101, and an upper top plug 103 is movably arranged inside the fixed feed bin 101; a constant temperature bin 207 is arranged in the middle of the culture bin 2, a linkage ring 212 is rotatably arranged on the inner wall of the culture bin 2, and the linkage ring 212 consists of two parts: a vertical tooth part 213 at the top and a gear ring part 214 at the bottom; a plurality of rotating seats 216 are arranged on the inner wall of the culture bin 2, and a rotating shaft 217 penetrating the constant temperature bin 207 is movably installed on the rotating seat 216, and the rotating shaft 217 is movably installed on the rotating seat 216. A linkage bevel gear 218 meshing with the vertical tooth portion 213 is installed in the middle of 17; the end of the rotating shaft 217 is connected to an active swing arm 219 located in the constant temperature bin 207, and the end of the active swing arm 219 is movably connected to a driven swing arm 220, and a lifting seat 221 is movably connected between the driven swing arms 220; a diluent bin 222 is arranged around the top of the lifting seat 221, and a moving ejector rod 224 cooperating with the upper ejector plug 103 is fixedly arranged in the middle of the lifting seat 221; a hollow moving unloading column 231 is also arranged on the lifting seat 221, and a fixed ejector rod 301 cooperating with it is arranged on the top of the unloading bin 3. When the lifting seat 221 reciprocates up and down, the mother liquid can be automatically unloaded and diluted for culture.

[0021] In this embodiment: the filtering and diluting device for phage culture is mainly divided into three parts: mother liquid bin 1, culture bin 2 and lower feed bin 3. When the device is in use, firstly, phage mother liquid is added to mother liquid bin 1 through feeding port 107, and phage diluent is added to diluent bin 222 through liquid adding port 227. When the mother liquid needs to be diluted and filtered, the lifting motor 236 is started through control panel 202. When lifting motor 236 drives linkage ring 212 to rotate, the active swing arm 219 drives the driven swing arm 220 to rotate under the drive of linkage bevel gear 218. Since the active swing arm 219 and the driven swing arm 220 located on the corresponding sides of lifting seat 221 are symmetrically installed, lifting seat 221 drives dilution chamber 223 to reciprocate up and down in constant temperature bin 207. When lifting seat 221 drives moving push rod 224 to move upward, moving push rod 224 will squeeze the upper plug 103 upward. After being squeezed, the upper top plug 103 will move upward relative to the fixed material discharge column 101, and the mother liquid in the mother liquid bin 1 will fall into the dilution chamber 223 below through the filter hole 102. When the lifting seat 221 moves downward, the moving top rod 224 will be separated from the upper top plug 103, at which time the filter hole 102 is blocked and the mother liquid will not fall anymore, and then the diluent is added to the dilution chamber 223 in proportion through the pump 225. When the diluted mother liquid needs to be discharged, when the lifting seat 221 drives the moving material discharge column 231 to move downward, the fixed top rod 301 will squeeze the lower top plug 233 upward. After being squeezed, the lower top plug 233 will move upward relative to the moving material discharge column 231, so the diluent in the dilution chamber 223 will fall into the drawer bin 304 below through the first liquid discharge trough 232 and the second liquid discharge trough 302.

[0022] See attached Figure 1 -Attached Figure 2 A positioning seat 203 cooperating with the mother liquid chamber 1 is arranged on the top of the culture chamber 2, an upper threaded portion 108 is opened on the bottom outer wall of the mother liquid chamber 1, and a lower threaded portion 204 is opened on the outer wall of the positioning seat 203; a threaded ring 205 is also sleeved on the outer wall of the positioning seat 203, and a plurality of groups of protrusions 206 are arranged on the outer wall of the threaded ring 205.

[0023] In this embodiment, in order to facilitate the rapid disassembly and assembly of the device, the mother liquid tank 1 is designed as a split structure, and the mother liquid tank 1 can be quickly installed on the positioning seat 203 through the threaded ring 205. In order to facilitate the rotation of the threaded ring 205, multiple groups of raised parts 206 are designed.

[0024] See attached Figure 1 -Attached Figure 2 The top of the upper plug 103 is connected to multiple sets of return springs 104, and the upper plug 103 is connected to the top of the fixed unloading column 101 through the return spring 104; a top cover 106 is installed on the top of the mother liquid bin 1, and a feeding port 107 is provided on the top cover 106.

[0025] In this embodiment: when the lifting seat 221 drives the moving top rod 224 to move upward, the moving top rod 224 will squeeze the upper top plug 103 upward. After being squeezed, the upper top plug 103 will move upward relative to the fixed material discharge column 101. Since the diameter of the moving top rod 224 is smaller than that of the upper top plug 103, the mother liquid in the mother liquid bin 1 will fall into the dilution chamber 223 below through the filter hole 102. When the lifting seat 221 moves downward, the moving top rod 224 is separated from the upper top plug 103. At this time, under the action of the return spring 104, the upper top plug 103 returns to its original position. At this time, the filter hole 102 is blocked and the mother liquid will not fall anymore.

[0026] See attached Figure 2 -Attached Figure 3 A mounting boss 210 is provided on the inner wall of the culture bin 2, a limit rail 211 is provided on the inner side of the mounting boss 210, and a T-shaped slot 215 matching with the linkage ring 212 is provided on the outer wall; a convex plate 235 is also provided on the inner wall of the culture bin 2, a lifting motor 236 is installed on the convex plate 235, and a driving gear 237 meshing with the gear ring part 214 is installed at the output end of the lifting motor 236.

[0027] In this embodiment, in order to install the linkage ring 212 and ensure that it is rotatably arranged on the inner wall of the culture chamber 2, a mutually matching limiting rail 211 and T-slot 215 structure are designed. In order to automatically drive the linkage ring 212 to rotate, thereby realizing automatic unloading of the mother liquid and discharge of the diluent, a lifting motor 236 structure is designed.

[0028] See attached Figure 2 -Attached Figure 3 A heating chamber 208 is provided inside the inner wall of the constant temperature bin 207 , and a plurality of heating tubes 209 are installed in the heating chamber 208 . A temperature sensor 303 located inside the constant temperature bin 207 is installed on the top of the lower bin 3 .

[0029] In this embodiment, in order to ensure that the temperature in the constant temperature chamber 207 is maintained at a temperature suitable for phage culture, multiple groups of heating tubes 209 are arranged inside the constant temperature chamber 207. In order to monitor the temperature inside the constant temperature chamber 207 in real time and further control the operation of the heating tubes 209, a temperature sensor 303 structure is designed.

[0030] See attached Figure 6 -Attached Figure 7A dilution chamber 223 of the mother liquid is formed between the dilution liquid bins 222, a pump 225 is also installed on the dilution liquid bin 222, and a discharge pipe 226 facing the dilution chamber 223 is provided at the output end of the pump 225, and a liquid filling port 227 is also provided at the top of the dilution liquid bin 222; a plurality of stirring motors 229 are installed at the bottom of the lifting seat 221, and a stirring fan 230 located in the dilution chamber 223 is provided at the output end of the stirring motor 229.

[0031] In this embodiment, when the diluent in the diluent bin 222 is used up, the mother liquid bin 1 on the top can be removed first, and the diluent can be added therein through the liquid adding port 227. In order to ensure that the phage mother liquid and the diluent are mixed evenly, multiple groups of stirring fans 230 are designed.

[0032] See attached Figure 2 and attached Figure 7 A first liquid level sensor 105 is arranged at the bottom of the mother liquid bin 1, and a second liquid level sensor 228 is arranged at the bottom of the dilution liquid bin 222; a plurality of groups of supporting legs 201 are arranged on the outer wall of the culture bin 2, and a control panel 202 is also arranged on the outer wall of the culture bin 2.

[0033] In this embodiment: In order to accurately control the amount of mother liquid added and the dilution ratio, multiple sets of sensors are designed. When the phage mother liquid falls from the mother liquid tank 1 into the dilution chamber 223, the first liquid level sensor 105 can monitor the liquid level in the mother liquid tank 1 in real time. Driven by the pump 225, the diluent in the dilution liquid tank 222 will be added to the dilution chamber 223 in proportion, and the second liquid level sensor 228 can monitor the liquid level in the dilution liquid tank 222 in real time. In order to control the operation of the device, a control panel 202 structure is designed.

[0034] See attached Figure 2 -Attached Figure 4 A first liquid row groove 232 is provided in the lower half of the movable material discharge column 231, and a lower top plug 233 is movably arranged inside the movable material discharge column 231; a plurality of sets of return springs 234 are connected to the top of the lower top plug 233, and the lower top plug 233 is connected to the top of the movable material discharge column 231 through the return springs 234; the fixed top rod 301 is a hollow structure, and a second liquid row groove 302 cooperating with the first liquid row groove 232 is provided on the outer wall of the upper half of the fixed top rod 301.

[0035] In this embodiment: when the lifting seat 221 drives the moving material discharge column 231 to move downward, the fixed push rod 301 will press the lower push plug 233 upward. After being squeezed, the lower push plug 233 will move upward relative to the moving material discharge column 231. Since the fixed push rod 301 and the lower push plug 233 have the same diameter, the diluent in the dilution chamber 223 will pass through the first liquid row trough 232 and the second liquid row trough 302 and fall into the lower drawer 304. When the lifting seat 221 moves upward and separates from the fixed push rod 301, the lower push plug 233 returns to its original position under the action of the second return spring 234. At this time, the first liquid row trough 232 is blocked and the diluent will not fall.

[0036] See attached Figure 1 -Attached Figure 2 The lower material bin 3 is internally provided with a drawer bin 304 located below the fixed top rod 301 , and a handle 305 is provided on the outer wall of the drawer bin 304 .

[0037] In this embodiment, when the phage mother solution is diluted, the diluent in the dilution chamber 223 will fall into the lower drawer 304 through the first and second liquid drain grooves 232 and 302. In order to facilitate the drawer, a handle 305 structure is designed.

[0038] See attached Figure 5 -Attached Figure 6 The active swing arm 219 and the driven swing arm 220 located on the corresponding two sides of the lifting seat 221 are symmetrically installed.

[0039] In this embodiment, when the linkage ring 212 rotates, in order to ensure that the lifting seat 221 rises and falls smoothly and avoids the occurrence of a jamming phenomenon, the active swing arm 219 and the driven swing arm 220 located on the corresponding sides of the lifting seat 221 are symmetrically installed.

[0040] Working principle of the present invention: When the device is in use, firstly, the phage mother liquid is added into the mother liquid bin 1 through the feeding port 107, and the phage diluent is added into the diluent bin 222 through the liquid feeding port 227. When the mother liquid needs to be diluted and filtered, the lifting motor 236 is started through the control panel 202. When the lifting motor 236 drives the linkage ring 212 to rotate, the active swing arm 219 drives the driven swing arm 220 to rotate under the drive of the linkage bevel gear 218. Since the active swing arm 219 and the driven swing arm 220 located on the corresponding sides of the lifting seat 221 are symmetrically installed, the lifting seat 221 drives the dilution chamber 223 to reciprocate up and down in the constant temperature bin 207. When the lifting seat 221 drives the moving push rod 224 to move upward, the moving push rod 224 will squeeze the upper plug 103 upward. After being squeezed, the upper top plug 103 will move upward relative to the fixed material discharge column 101, and the mother liquid in the mother liquid bin 1 will fall into the dilution chamber 223 below through the filter hole 102. When the lifting seat 221 moves downward, the moving top rod 224 will be separated from the upper top plug 103, at which time the filter hole 102 is blocked and the mother liquid will not fall anymore, and then the diluent is added to the dilution chamber 223 in proportion through the pump 225. When the diluted mother liquid needs to be discharged, when the lifting seat 221 drives the moving material discharge column 231 to move downward, the fixed top rod 301 will squeeze the lower top plug 233 upward. After being squeezed, the lower top plug 233 will move upward relative to the moving material discharge column 231, so the diluent in the dilution chamber 223 will fall into the drawer bin 304 below through the first liquid discharge trough 232 and the second liquid discharge trough 302.

[0041] The above description of the disclosed embodiments enables those skilled in the art to implement or use the present invention. Various modifications to these embodiments will be apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to the embodiments shown herein, but will conform to the widest scope consistent with the principles and inventive features disclosed herein.

Claims

1. A filtering and dilution device for phage culture, comprising a mother liquid bin (1), a culture bin (2) and a feed bin (3), characterized in that: The mother liquid bin (1), the culture bin (2) and the feed bin (3) are assembled in sequence from top to bottom; a hollow fixed feed bin (101) is fixedly arranged in the middle of the mother liquid bin (1), a filter hole (102) is provided in the lower half of the fixed feed bin (101), and an upper top plug (103) is movably arranged inside the fixed feed bin (101); a constant temperature bin (207) is arranged in the middle of the culture bin (2), and a linkage ring (212) is rotatably arranged on the inner wall of the culture bin (2), and the linkage ring (212) consists of two vertical teeth (213) at the top and a gear ring (214) at the bottom. The culture chamber (2) is composed of a plurality of rotating seats (216) arranged on the inner wall of the culture chamber (2), a rotating shaft (217) penetrating the constant temperature chamber (207) being movably mounted on the rotating seat (216), a linkage bevel tooth (218) meshing with the vertical tooth portion (213) being mounted in the middle of the rotating shaft (217); an active swing arm (219) located in the constant temperature chamber (207) is connected to the end of the rotating shaft (217), a driven swing arm (220) is movably connected to the end of the active swing arm (219), and a lifting seat (221) is movably connected between the driven swing arms (220); A diluent bin (222) is arranged around the top of the lifting seat (221), and a movable ejector rod (224) cooperating with the upper ejector plug (103) is fixedly arranged in the middle of the lifting seat (221); a hollow movable material discharge column (231) is also arranged on the lifting seat (221), and a fixed ejector rod (301) cooperating with the material discharge bin (3) is arranged on the top of the lifting seat (221); when the lifting seat (221) reciprocates up and down, the mother liquid can be automatically discharged and diluted for culture.

2. A filtration and dilution device for phage culture according to claim 1, characterized in that: A positioning seat (203) cooperating with the mother liquid chamber (1) is arranged at the top of the culture chamber (2); an upper threaded portion (108) is provided on the outer wall of the bottom of the mother liquid chamber (1); a lower threaded portion (204) is provided on the outer wall of the positioning seat (203); a threaded ring (205) is also sleeved on the outer wall of the positioning seat (203); and a plurality of groups of protrusions (206) are provided on the outer wall of the threaded ring (205).

3. A filtration and dilution device for phage culture according to claim 1, characterized in that: The top of the upper ejector plug (103) is connected to a plurality of sets of return springs (104), and the upper ejector plug (103) is connected to the top of the fixed discharge column (101) via the return springs (104); a top cover (106) is installed on the top of the mother liquid bin (1), and a feeding port (107) is provided on the top cover (106).

4. A filtration and dilution device for phage culture according to any one of claims 1 to 3, characterized in that: The inner wall of the culture bin (2) is provided with a mounting boss (210), the inner side of the mounting boss (210) is provided with a limit rail (211), and the outer wall of the linkage ring (212) is provided with a T-shaped slot (215) that cooperates with the mounting boss; the inner wall of the culture bin (2) is also provided with a convex plate (235), a lifting motor (236) is mounted on the convex plate (235), and the output end of the lifting motor (236) is provided with a driving gear (237) that meshes with the gear ring (214).

5. A filtration and dilution device for phage culture according to claim 1, characterized in that: A heating chamber (208) is provided inside the inner wall of the constant temperature bin (207), and a plurality of groups of heating tubes (209) are installed in the heating chamber (208). A temperature sensor (303) located inside the constant temperature bin (207) is installed on the top of the lower bin (3).

6. A filtration and dilution device for phage culture according to any one of claims 1 to 3, characterized in that: A mother liquid dilution chamber (223) is formed between the dilution liquid bins (222); a pump (225) is also installed on the dilution liquid bin (222); an output end of the pump (225) is provided with a liquid discharge pipe (226) facing the dilution chamber (223); and a liquid filling port (227) is also provided on the top of the dilution liquid bin (222); a plurality of stirring motors (229) are installed at the bottom of the lifting seat (221); a stirring fan (230) located in the dilution chamber (223) is provided at the output end of the stirring motor (229).

7. A filtration and dilution device for phage culture according to any one of claims 1 to 3, characterized in that: A first liquid level sensor (105) is arranged at the bottom of the mother liquid bin (1), and a second liquid level sensor (228) is arranged at the bottom of the dilution liquid bin (222); a plurality of groups of supporting legs (201) are arranged on the outer wall of the culture bin (2), and a control panel (202) is also arranged on the outer wall of the culture bin (2).

8. A filtration and dilution device for phage culture according to claim 1, characterized in that: The lower half of the movable material discharge column (231) is provided with a first liquid discharge groove (232), and a lower top plug (233) is movably arranged inside the movable material discharge column (231); the top of the lower top plug (233) is connected to a plurality of sets of second return springs (234), and the lower top plug (233) is connected to the top of the movable material discharge column (231) via the second return springs (234); the fixed top rod (301) is a hollow structure, and a second liquid discharge groove (302) cooperating with the first liquid discharge groove (232) is provided on the outer wall of the upper half of the fixed top rod (301).

9. A filtering and diluting device for phage culture according to any one of claims 1 to 3, characterized in that: The lower material bin (3) is movably provided with a drawer bin (304) located below the fixed top rod (301), and a handle (305) is provided on the outer wall of the drawer bin (304).

10. The filtration and dilution device for phage culture according to claim 1, characterized in that: The active swing arm (219) and the driven swing arm (220) located on the corresponding two sides of the lifting seat (221) are symmetrically installed.

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