Detection device for tolerance of pathogenic microorganisms

Abstract

The invention relates to the technical field of pathogenic microorganism detection, provides a pathogenic microorganism tolerance detection device, and solves the technical problem that the existing device is difficult to carry out multi-temperature and multi-medicament concentration tolerance detection at the same time. The device comprises a detection box, the interior of the detection box is divided into three detection cavities through transverse partition plates, each detection cavity and a box top fixing base are each provided with a rotatable placing table, the detection cavity placing tables are used for placing culture dishes, and the box top placing table is used for placing test tubes with different medicament concentrations through pearl wool bases; the detection box is provided with a medicament adding mechanism, and medicaments in test tubes can be quantitatively atomized and added into the culture dishes; a heating mechanism is arranged on one side, and different temperature environments are constructed for the three detection cavities in combination with a temperature sensor and a flow regulating valve. The pathogenic microorganism tolerance detection device provided by the scheme integrates culture and medicament adding functions, realizes multivariable synchronous detection, simplifies the operation process, reduces the pollution risk, and improves the detection efficiency and accuracy.

Description

Technical Field

[0001] This invention relates to the field of pathogenic microorganism detection technology, and in particular to a pathogenic microorganism resistance detection device. Background Technology

[0002] Pathogen resistance testing is a crucial step in clinical medicine, microbiology research, and disease control. Its core purpose is to determine the tolerance of pathogens to different agents, providing a scientific basis for clinical medication regimens and drug development. Currently, pathogen resistance testing typically requires the simultaneous culturing of multiple samples and the precise addition of agents to the culture system.

[0003] Existing detection devices are unable to simultaneously perform resistance testing on pathogenic microorganisms at multiple temperatures and different reagent concentrations.

[0004] Therefore, it is necessary to provide a pathogen resistance detection device to solve the above-mentioned technical problems. Summary of the Invention

[0005] This invention provides a pathogen resistance detection device, aiming to solve the technical problem of simultaneously detecting pathogen resistance under different temperatures and different reagent concentrations.

[0006] This invention is implemented as follows: a pathogen resistance testing device includes: a testing box with two transverse partitions inside, dividing the interior of the testing box into three identical testing chambers; wherein, a fixing seat is fixedly installed on the inner wall of the bottom of each of the three testing chambers and on the top of the testing box, and a rotatable placement platform is installed on each fixing seat; the placement platform in each testing chamber has several annularly spaced placement slots for placing pathogen culture dishes; a pearl cotton seat for placing test tubes is installed on the placement platform on the top of the testing box, and the pearl cotton seat has several slots for placing test tubes of different reagent concentrations; in addition, the testing box is also equipped with a reagent adding mechanism for adding reagents to the culture dishes; a heating mechanism is also installed on one side of the testing box for heating the three testing chambers, providing different temperature culture environments for the pathogens in the three testing chambers.

[0007] Preferably, the reagent addition mechanism includes a metering pump fixed to one side of the detection chamber. The inlet pipe of the metering pump is connected to a vertically arranged first telescopic pipe, and the other end of the first telescopic pipe is connected to a suction pipe for drawing reagents into the test tube. The outlet pipe of the metering pump is connected to a vertically arranged second telescopic pipe, and the bottom end of the second telescopic pipe is connected to a vertical pipe that is vertically slidably connected to two horizontal partitions. Three mounting pipes located in three detection chambers are installed on the vertical pipe. One end of the mounting pipe is equipped with an atomizing nozzle for adding reagents into the petri dish.

[0008] Preferably, an electric telescopic rod is installed on the bottom inner wall of the detection box, the top end of the output rod of the electric telescopic rod is fixedly connected to the bottom of the vertical tube, and a vertical rod is fixedly installed on the installation tube in the uppermost detection chamber, and a connecting plate for fixing the liquid extraction tube is fixedly installed on the top end of the vertical rod.

[0009] Preferably, each of the four fixed seats is equipped with a rotating mechanism, and the three rotating mechanisms inside the detection box are equipped with a common drive mechanism. The rotating mechanism above the detection box is equipped with a linkage mechanism for driving the rotation of the placement stage to switch between petri dishes and test tubes with different reagent concentrations in different positions. The rotating mechanism includes: a first spline sleeve that is rotatably mounted on the fixed seat and is vertically arranged via bearings; a first spline shaft that extends to and meshes with the first spline sleeve and is fixedly mounted on the bottom of the placement stage; a worm gear that is fixedly sleeved on the first spline sleeve and meshes with a worm that is rotatably mounted on the fixed seat; and a first bevel gear that is fixedly sleeved on one end of the worm and is located outside the detection box.

[0010] Preferably, the top of the fixing base and the bottom of the placement platform are provided with matching annular grooves, and multiple balls are provided in the annular grooves of the fixing base to support the placement platform and ensure its stable rotation.

[0011] Preferably, the drive mechanism includes a stepper motor fixed to one side of the housing. The dual-axis motor is a dual-output-axis motor. Both output shafts of the stepper motor are fixedly connected to a connecting shaft via a coupling. The connecting shaft is rotatably connected to a bearing seat fixed to one side of the detection housing via a bearing. A second bevel gear is fixedly sleeved on each connecting shaft (two second bevel gears are sleeved on the lower connecting shaft). The second bevel gear meshes with the corresponding first bevel gear.

[0012] Preferably, the linkage mechanism includes a rotating shaft rotatably mounted on two bearing seats fixed on one side of the detection box via bearings. A second bevel gear is fixedly sleeved on the top end of the rotating shaft and meshes with a first bevel gear above the detection box. A second spline shaft is provided at the bottom end of the rotating shaft and the top end of the corresponding connecting shaft. A second spline sleeve that can slide vertically is slidably sleeved on the two second spline shafts, and the second spline sleeve meshes with the two second spline shafts.

[0013] Preferably, a moving mechanism is installed on one side of the testing box for moving the second spline sleeve to switch the disengagement and engagement of the second spline sleeve with the second spline shaft at the top of the connecting shaft. The moving mechanism includes: a support seat sleeved on the second spline sleeve and rotatably connected to the second spline sleeve via a bearing; two limiting slide rods fixed on one side of the testing box and vertically arranged, with the support seat slidably sleeved on the two limiting slide rods; and an electric push rod fixedly installed on a bearing seat, with the bottom end of the output rod of the electric push rod fixedly connected to the top of the support seat.

[0014] Preferably, the heating mechanism includes: a blower fixed to the side wall of the detection chamber; a heating box is installed at the air outlet of the blower and communicates with the inside of the heating box; a heating wire is installed inside the heating box and equipped with a thermostat to adjust the heating power; an air outlet is provided at the top of the heating box and connected to a multi-port pipe; the other three ports of the multi-port pipe are all connected to heat delivery pipes; each heat delivery pipe is equipped with a flow regulating valve; fixed horizontal pipes are fixedly installed on the top inner wall of the detection chamber and at the bottom of the two horizontal partitions; a sleeve is installed and connected to the bottom of the fixed horizontal pipe; a rotating pipe is rotatably installed on the bottom inner wall of the sleeve through a sealed bearing; a horizontally arranged jet pipe is fixed and connected to the bottom end of the rotating pipe; two rows of jet holes are opened on both sides of the jet pipe, and the two rows of jet holes are located on both sides of the rotating pipe. The purpose of this design is to make the jet pipe and the rotating pipe rotate due to the reaction force of the airflow when the jet hole sprays hot air, thereby making the hot air evenly distributed in the detection chamber.

[0015] Preferably, a first protective cover is installed on the top of the testing box, the test tube is located inside the first protective cover, and a second protective cover and a third protective cover are installed on both sides of the testing box respectively. The driving mechanism and the linkage mechanism are located inside the second protective cover, and the heating mechanism is located inside the third protective cover.

[0016] Preferably, each of the detection chambers is equipped with a patch-type temperature sensor on its inner wall for monitoring the patch-type temperature sensor and cooperating with the temperature controller to regulate the ambient temperature inside the three detection chambers.

[0017] Preferably, each of the side walls of the detection chamber is provided with a vent hole, and a check valve is installed on the vent hole. The check valve can prevent cold air from outside the detection chamber from entering the detection chamber.

[0018] Preferably, each of the detection chambers is equipped with a germicidal lamp on its inner wall to sterilize the environment inside the detection chamber before culturing pathogenic microorganisms.

[0019] Preferably, a support frame is installed on the bottom of the partition and the top inner wall of the detection box, and a wide-angle camera is installed on the support frame to monitor the culture of pathogenic microorganisms in the petri dish.

[0020] Preferably, each of the placement platforms is fixedly equipped with a handle for picking up the platform, so as to facilitate the simultaneous picking up and placing of multiple petri dishes or multiple test tubes.

[0021] Compared with related technologies, the pathogen resistance detection device provided by this invention has the following beneficial effects: This solution provides a resistance detection device that integrates microbial biological culture, drug storage and addition structures, which simplifies the process, saves space and reduces the risk of contamination; This solution supports multivariate detection and realizes the simultaneous detection of temperature and drug concentration variables. Attached Figure Description

[0022] Figure 1 This is a schematic diagram of the structure of a pathogen resistance detection device provided by the present invention; Figure 2 This is a frontal cross-sectional view of a pathogen resistance detection device provided by the present invention; Figure 3 for Figure 2 An enlarged structural diagram of part A shown in the figure; Figure 4 for Figure 2 An enlarged structural diagram of part B shown in the figure; Figure 5 for Figure 2 An enlarged structural diagram of section C shown in the figure; Figure 6 for Figure 2 An enlarged structural diagram of part D shown in the figure; Figure 7 for Figure 2 An enlarged structural diagram of part E shown in the figure; Figure 8 This is a top view of the fixed base in this invention. Figure 9 This is a top view of the placement platform in this invention. Figure 10 This is a top view of the pearl cotton seat in this invention. Figure 11 This is a side view of the support structure in this invention; Figure 12 This is a top view of the jet pipe structure in this invention; Figure 13 This is a side view of the limiting slide bar in this invention.

[0023] Labels in the diagram: 1. Detection box; 2. Horizontal partition; 3. Detection chamber; 4. Fixing base; 5. Placement stage; 6. Petri dish; 7. First protective cover; 8. Test tube; 9. Metering pump; 10. Inlet pipe; 11. First telescopic pipe; 12. Suction pipe; 13. Outlet pipe; 14. Vertical pipe; 15. Mounting pipe; 16. Atomizing nozzle; 17. Electric telescopic rod; 18. Vertical rod; 19. Connecting plate; 20. First spline sleeve; 21. First spline shaft; 22. Worm gear; 23. Worm; 24. Stepper motor; 25. Connecting shaft; 26. Bearing seat; 27. First conical tooth 28. Wheel; 29. ​​Second bevel gear; 30. Shaft; 31. Second spline sleeve; 32. Support base; 33. Limiting slide rod; 34. Electric push rod; 35. Second protective cover; 36. Third protective cover; 37. Blower; 38. Heating box; 39. Heating wire; 40. Multi-port pipe; 41. Heat supply pipe; 42. Fixed horizontal pipe; 43. Sleeve; 44. Rotating pipe; 45. Jet pipe; 46. Jet hole; 47. Surface mount temperature sensor; 48. Vent hole; 49. Germicidal lamp; 50. Wide-angle camera; 51. Support frame; 52. Handle; 83. Pearl cotton seat. Detailed Implementation

[0024] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs; the terminology used herein in the specification is for the purpose of describing particular embodiments only and is not intended to be limiting of this application; the terms "comprising" and "having," and any variations thereof, in the specification and the foregoing drawings are intended to cover non-exclusive inclusion. The terms "first," "second," etc., in the specification and the foregoing drawings are used to distinguish different objects, not to describe a particular order.

[0025] In this document, the term "embodiment" means that a particular feature, structure, or characteristic described in connection with an embodiment may be included in at least one embodiment of this application. The appearance of this phrase in various places throughout the specification does not necessarily refer to the same embodiment, nor is it a separate or alternative embodiment mutually exclusive with other embodiments. It will be explicitly and implicitly understood by those skilled in the art that the embodiments described herein can be combined with other embodiments.

[0026] This invention provides a pathogen resistance detection device, such as... Figure 1-13As shown, the pathogen resistance testing device includes: a testing chamber 1 with two internal partitions 2, which divide the interior of the testing chamber 1 into three identical testing chambers 3; a fixing seat 4 is fixedly installed on the inner wall of the bottom of each of the three testing chambers 3 and on the top of the testing chamber 1, and a rotatable placement platform 5 is installed on each fixing seat 4; the placement platform 5 in each testing chamber 3 has several annularly spaced placement slots for placing pathogenic microorganism culture dishes 6; a pearl cotton seat 81 for placing test tubes 8 is installed on the placement platform 5 on the top of the testing chamber 1, and the pearl cotton seat 81 has several slots for placing test tubes 8 with different reagent concentrations; in addition, the testing chamber 1 is also equipped with a reagent adding mechanism for adding reagents to the culture dishes; a heating mechanism is also installed on one side of the testing chamber 1 to heat the three testing chambers 3, providing different temperature culture environments for the pathogenic microorganisms in the three testing chambers 3.

[0027] In this embodiment, during use, the placement platform 5 containing the pathogenic microorganism culture dish 6 is first placed on the fixed base 4, and the placement platform containing test tubes 8 with different drug concentrations is placed on the fixed base 4 on top of the detection chamber 1. A heating mechanism provides different temperature environments for the three detection chambers 3. The drug addition mechanism adds the drug from the test tubes 8 to the culture dish 6. Simultaneously, the rotation of the placement platform 5 ensures uniform drug addition and heating of multiple culture dishes 6. This invention integrates culture, drug storage, and addition functions, enabling simultaneous detection of multiple temperatures and drug concentrations, simplifying the operation process, saving space, reducing the risk of contamination, and improving detection efficiency and result accuracy.

[0028] In a further preferred embodiment of the present invention, the reagent addition mechanism includes a metering pump 9 fixed to one side of the detection chamber 1. The inlet pipe 10 of the metering pump 9 is connected to a vertically arranged first telescopic pipe 11. The other end of the first telescopic pipe 11 is connected to a suction pipe 12 for drawing reagents into the test tube 8. The outlet pipe 13 of the metering pump 9 is connected to a vertically arranged second telescopic pipe. The bottom end of the second telescopic pipe is connected to a vertical pipe 14 that is vertically slidably connected to two horizontal partitions 2. Three mounting pipes 15 are installed on the vertical pipe 14, which are respectively located in the three detection chambers 3. One end of the mounting pipe 15 is equipped with an atomizing nozzle 16 for adding reagents into the petri dish 6.

[0029] In this embodiment, the quantitative pump 9 drives the extraction tube 12 to enter the test tube 8 through the inlet tube 10 and the first telescopic tube 11 to extract the reagent. The reagent is then transported to the vertical tube 14 through the outlet tube 13 and the second telescopic tube, and then sprayed into the petri dish 6 through the atomizing nozzle 16 via the mounting tube 15 located in the three detection chambers 3 on the vertical tube 14. This achieves precise quantitative extraction and delivery of reagents of different concentrations. The atomizing nozzle 16 ensures that the reagent is evenly distributed in the petri dish 6 and can simultaneously add reagents to the petri dishes 6 in the three detection chambers 3, improving the efficiency and uniformity of the dosing and ensuring the accuracy of the detection results.

[0030] In a further preferred embodiment of the present invention, an electric telescopic rod 17 is installed on the bottom inner wall of the detection box 1. The top end of the output rod of the electric telescopic rod 17 is fixedly connected to the bottom of the vertical tube 14, and a vertical rod 18 is fixedly installed on the mounting tube 15 in the uppermost detection chamber 3. A connecting plate 19 for fixing the liquid extraction tube 12 is fixedly installed at the top end of the vertical rod 18.

[0031] In this embodiment, the extension and retraction of the electric telescopic rod 17 can drive the vertical tube 14 to move vertically, and then drive the liquid extraction tube 12 to rise and fall synchronously through the vertical rod 18 and the connecting plate 19. At the same time as the liquid extraction tube 12 extends into the test tube 8, the atomizing nozzle 16 automatically approaches the petri dish 6, so that the agent can be accurately and evenly injected into the surface of the pathogenic microbial culture medium in the petri dish 6.

[0032] In another embodiment of the present invention, a rotating mechanism is installed on each of the four fixed seats 4, and the three rotating mechanisms inside the detection box 1 are equipped with a common driving mechanism. The rotating mechanism above the detection box 1 is equipped with a linkage mechanism for driving the rotation of the placement stage 5 to switch the petri dishes 6 in different positions and the test tubes 8 with different reagent concentrations. The rotating mechanism includes: a first spline sleeve 20 that is rotatably mounted on the fixed seat 4 and is vertically arranged; a first spline shaft 21 that extends to and meshes with the first spline sleeve 20 is fixedly installed at the bottom of the placement stage 5; a worm gear 22 that is fixedly sleeved on the first spline sleeve 20 and meshes with a worm 23 that is rotatably mounted on the fixed seat 4; and a first bevel gear 27 that is fixedly sleeved on one end of the worm 23 and is located outside the detection box 1.

[0033] In this embodiment, the drive mechanism drives the first bevel gear 27 of the rotating mechanism inside the detection chamber 1 to rotate. This rotation, via the meshing of the worm gear 23 and worm wheel 22, drives the first spline sleeve 20 to rotate. The meshing of the first spline sleeve 20 and the first spline shaft 21 drives the placement platform 5 inside the detection chamber 3 to rotate. The linkage mechanism then drives the placement platform 5 above the detection chamber 1 to rotate, enabling the switching of petri dishes 6 at different positions and test tubes 8 with different reagent concentrations. This ensures stable and precise rotation of the placement platform 5, allowing for uniform drug addition and heating of all petri dishes 6. Simultaneously, it facilitates convenient and synchronous switching of test tubes 8 to add different concentrations of reagent, improving detection efficiency and experimental uniformity, and ensuring the reliability of detection results. Furthermore, it facilitates the placement of the placement platform 5, thereby allowing for the simultaneous handling of multiple petri dishes 6 or multiple test tubes 8.

[0034] In a further preferred embodiment of the present invention, the top of the fixed base 4 and the bottom of the placement platform 5 are both provided with matching annular grooves, and a plurality of balls are provided in the annular grooves of the fixed base 4 to support the placement platform 4 and ensure its stable rotation.

[0035] In this embodiment, during the rotation of the placement platform 5, the ball bearings in the annular groove of the fixed seat 4 can provide stable support for the placement platform 5 and assist in rolling, reducing the frictional resistance when the placement platform 5 rotates, avoiding rotation jamming, further improving the stability and smoothness of the rotation of the placement platform 5, ensuring the safe placement of the culture dish 6 and test tube 8, and ensuring the stability and reliability of the switching and drug addition process.

[0036] In another embodiment of the present invention, the driving mechanism includes a stepper motor 24 fixed on one side of the housing 1. The dual-axis motor 24 is a dual-output shaft motor. The model used in this solution is 28BYJ-48. The two output shafts of the stepper motor 24 are fixedly connected to a connecting shaft 25 through a coupling. The connecting shaft 25 is rotatably connected to a bearing seat 26 fixed on one side of the detection housing 1 through a bearing. A second bevel gear 28 is fixedly sleeved on each connecting shaft 25 (two second bevel gears 28 are sleeved on the lower connecting shaft 25). The second bevel gear 28 meshes with the corresponding first bevel gear 27.

[0037] In this embodiment, the stepper motor 24 is started, and its dual output shafts drive the connecting shaft 25 to rotate on the bearing seat 26 through the coupling. The second bevel gear 28 on the connecting shaft 25 meshes with the first bevel gear 27 of the rotating mechanism to drive the three placement platforms 5 in the detection box 1 to rotate synchronously. This realizes the synchronous driving of multiple placement platforms 5 to rotate, improves the rotation control accuracy and transmission stability, and ensures the synchronous and uniform drug addition and switching efficiency of multiple culture dishes 6.

[0038] In another embodiment of the present invention, the linkage mechanism includes a rotating shaft 29 rotatably mounted on two bearing seats 26 fixed on one side of the detection box 1 via bearings. A second bevel gear 28 is fixedly sleeved on the top end of the rotating shaft 29 and meshes with a first bevel gear 27 above the detection box 1. A second spline shaft is provided at the bottom end of the rotating shaft 29 and the top end of the corresponding connecting shaft 25. A second spline sleeve 30 that can slide vertically is slidably sleeved on the two second spline shafts and meshes with the two second spline shafts.

[0039] In this embodiment, when it is necessary to drive the upper placement platform 5 of the test box 1 to rotate, the second spline sleeve 30 meshes with the second spline shaft at the bottom end of the rotating shaft 29 and the top end of the connecting shaft 25. The power of the connecting shaft 25 is transmitted to the rotating shaft 29 through the second spline sleeve 30, and then meshes with the first bevel gear 27 of the rotating mechanism above the test box 1 through the second bevel gear 28 at the top end of the rotating shaft 29, thereby driving the upper placement platform 5 to rotate. This solution can synchronously drive the upper placement platform 5 to rotate with the power of the driving mechanism, without the need for additional driving components, saving costs, while ensuring the accuracy and stability of the test tube 8 switching.

[0040] In another embodiment of the present invention, a moving mechanism is installed on one side of the detection box 1, used to move the second spline sleeve 30 up and down to switch the disengagement and engagement of the second spline sleeve 30 with the second spline shaft at the top of the connecting shaft 25. The moving mechanism includes: a support base 31 sleeved on the second spline sleeve 30 and rotatably connected to the second spline sleeve 30 via bearings; two limiting slide rods 32 fixed on one side of the detection box 1 and vertically arranged, the support base 31 being slidably sleeved on the two limiting slide rods 32; and an electric push rod 33 fixedly installed on a bearing seat 26, the bottom end of the output rod of the electric push rod 33 being fixedly connected to the top of the support base 31. This solution realizes the disengageable transmission of power. In this embodiment, by controlling the extension and retraction of the electric push rod 33, the support base 31 is driven to rise and fall along the two vertical limiting slide rods 32, thereby driving the second spline sleeve 30, which is connected to the support base 31 through the bearing, to rise and fall synchronously, realizing the disengagement and engagement switching of the second spline sleeve 30 and the second spline shaft at the top of the connecting shaft 25; this scheme realizes the disengagement and engagement of power, and can flexibly control the rotation start and stop of the platform 5 above the detection box 1, which is suitable for situations where only the same reagent concentration is needed to simultaneously detect multiple groups of pathogens and microorganisms.

[0041] In another embodiment of the present invention, the heating mechanism includes: a blower 36 fixed to the side wall of the detection box 1; a heating box 37 is installed at the air outlet of the blower 36 and communicates with the interior of the heating box 37; a heating wire 38 is installed inside the heating box 37 and equipped with a thermostat to adjust the heating power; an air outlet is provided at the top of the heating box 37 and connected to a multi-port pipe 39; the other three ports of the multi-port pipe 39 are all connected to heat delivery pipes 40; each heat delivery pipe 40 is equipped with a flow regulating valve; and the top inner wall of the detection box 1 and the bottom of the two transverse partitions 2 are fixed. A fixed horizontal tube 41 is installed, and a sleeve 42 is installed and connected to the bottom of the fixed horizontal tube 41. A rotating tube 43 is rotatably installed on the inner wall of the bottom end of the sleeve 42 through a sealed bearing. A horizontally arranged jet pipe 44 is fixed and connected to the bottom end of the rotating tube 43. Two rows of jet holes 45 are opened on both sides of the jet pipe 44, and the two rows of jet holes 45 are respectively located on both sides of the rotating tube 43. The purpose of this design is to make the jet pipe 44 and the rotating tube 43 rotate when hot air is ejected from the jet holes 45, so that the hot air is evenly distributed in the detection chamber 3.

[0042] In this embodiment, the blower 36 is activated to send airflow into the heating box 37, the heating wire 38 heats the airflow, the thermostat adjusts the heating power, and the hot airflow is split into each heating pipe 40 through the multi-pass pipe 39. The airflow in each detection chamber 3 is controlled by adjusting the flow regulating valve on the heating pipe 40. The hot airflow then enters the rotating pipe 43 through the fixed horizontal pipe 41 and the sleeve 42, and is ejected from the air jet holes 45 on both sides of the jet pipe 44. The airflow reaction force drives the jet pipe 44 and the rotating pipe 43 to rotate. The airflow in each detection chamber 3 is controlled by three flow regulating valves, thereby realizing the construction of different temperature environments in the three detection chambers 3. The rotating jet pipe 44 makes the hot air evenly distributed, ensuring that the temperature in each detection chamber 3 is balanced and stable, providing a reliable environment for the multi-temperature gradient culture of pathogenic microorganisms and improving the accuracy of the detection results.

[0043] In a further preferred embodiment of the present invention, a first protective cover 7 is installed on the top of the detection box 1, the test tube 8 is located inside the first protective cover 7, and a second protective cover 34 and a third protective cover 35 are respectively installed on both sides of the detection box 1. The driving mechanism and the linkage mechanism are located inside the second protective cover 34, and the heating mechanism is located inside the third protective cover 35.

[0044] In this embodiment, the test tube 8 is placed inside the first protective cover 7 on top of the test chamber 1 to prevent the equipment and the reagents inside the test tube 8 from being contaminated by the outside during the test. The drive mechanism and linkage mechanism are housed inside the second protective cover 34, and the heating mechanism is housed inside the third protective cover 35. This provides protection for the drive, linkage and heating components, preventing damage from external forces and accidental contact by personnel. It also reduces interference during the operation of the components, ensuring the overall safe operation of the device and the cleanliness of the testing environment.

[0045] In a further preferred embodiment of the present invention, each of the detection chambers 3 is provided with a patch temperature sensor 46 for monitoring the patch temperature sensor. The model used in this solution is TMP1075, which works in conjunction with a temperature controller to regulate the ambient temperature inside the three detection chambers 3.

[0046] In this embodiment, a patch-type temperature sensor 46 is used to monitor the temperature inside the chamber in real time and feed the temperature signal back to the temperature controller. The temperature controller adjusts the heating power of the heating mechanism accordingly to ensure that the temperature of each detection chamber 3 is stable within the set range, thereby improving the accuracy of the multi-temperature gradient culture environment and further ensuring the reliability of the detection results.

[0047] In a further preferred embodiment of the present invention, each side wall of the detection chamber 3 is provided with a vent hole 47, and a check valve is installed on the vent hole 47. The check valve can prevent cold air outside the detection box 1 from entering the detection chamber 3.

[0048] In this embodiment, when the gas pressure in the detection chamber 3 is too high due to the input of hot air, the gas can be discharged through the vent 47 to balance the gas pressure. The check valve on the vent 47 prevents cold air from entering and disrupting the temperature stability inside the chamber, thus ensuring the continuity and stability of the multi-temperature gradient culture environment.

[0049] In a further preferred embodiment of the present invention, a germicidal lamp 48 is installed on the inner wall of the detection chamber 3 to sterilize the environment inside the detection chamber 3 before culturing pathogenic microorganisms.

[0050] In this embodiment, the germicidal lamp 48 on the inner wall of the detection chamber 3 is turned on before the pathogenic microorganism is cultured to sterilize the environment inside the detection chamber 3; its beneficial effect is that it can effectively remove miscellaneous bacteria inside the detection chamber 3 and avoid a miscellaneous environment inside the chamber.

[0051] It is worth noting that the circuits, electronic components, and modules involved in this invention are all existing technologies, which can be fully implemented by those skilled in the art, and need not be elaborated upon. The content protected by this invention does not involve improvements to the software and methods. The control method of this application is automatic control through a controller. The control circuit of the controller can be implemented by simple programming by those skilled in the art. This solution also includes a control panel, through which parameters can be set. The power connection methods of each electrical device are existing mature technologies and are well known to those skilled in the art, and will not be described in detail here.

[0052] In summary, compared with related technologies, the pathogen resistance detection device provided by this invention has significant advantages: First, by cooperating with the heating mechanism in the three independent detection chambers within the detection chamber, combined with the precise control of the flow regulating valve and temperature sensor, simultaneous multi-temperature gradient culture can be achieved, solving the problem that traditional devices cannot simultaneously perform multi-temperature detection; Second, the integrated structure of reagent storage, quantitative addition, and rotating placement platform allows for simultaneous and precise addition of multiple reagent concentrations and sample switching, simplifying the operation process and reducing the risk of contamination; Third, the design of each protective and monitoring component ensures the stability of device operation and the cleanliness of the detection environment, significantly improving detection efficiency and result accuracy, and is suitable for pathogen resistance detection needs in multiple fields such as clinical medicine and microbiology research.

[0053] It should be understood, in the several embodiments provided in this application, that the disclosed apparatus may be implemented in other ways.

[0054] The above embodiments are only used to illustrate the technical solutions of the present invention, and are not intended to limit the scope of protection of the invention. Obviously, the described embodiments are only some embodiments of the present invention, not all embodiments. Based on these embodiments, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention. Although the present invention has been described in detail with reference to the above embodiments, those skilled in the art can still combine, add, delete, or otherwise adjust the features of the various embodiments of the present invention according to the circumstances without conflict or creative effort, thereby obtaining different technical solutions that do not fundamentally depart from the concept of the present invention. These technical solutions also fall within the scope of protection of the present invention.

Claims

1. A pathogen resistance detection device, characterized in that, include: The test box has two horizontal partitions installed inside, which divide the interior of the test box into three identical test chambers; Among them, the bottom inner walls of the three detection chambers and the top of the detection box are fixedly installed with a fixed base. The fixed base is equipped with a rotatable placement platform. The placement platform in the detection chamber is provided with several ring-shaped and equally spaced placement slots for placing pathogenic microorganism culture dishes. The test chamber is equipped with a pearl cotton holder on the top platform for placing test tubes. The pearl cotton holder has several slots for placing test tubes with different reagent concentrations. In addition, the detection box is also equipped with a reagent addition mechanism for adding reagents to petri dishes; A heating mechanism is also installed on one side of the detection chamber to heat the three detection chambers and provide different temperature culture environments for the pathogenic microorganisms in the three detection chambers.

2. The pathogen resistance detection device according to claim 1, characterized in that, The reagent addition mechanism includes a metering pump fixed to one side of the detection chamber. The inlet pipe of the metering pump is connected to a vertically arranged first telescopic tube, and the other end of the first telescopic tube is connected to a suction tube for drawing reagents into the test tube. The outlet pipe of the metering pump is connected to a vertically arranged second telescopic tube. The bottom end of the second telescopic tube is connected to a vertical tube that is vertically slidably connected to two horizontal partitions. Three mounting tubes located in three detection chambers are installed on the vertical tube. One end of the mounting tube is equipped with an atomizing nozzle for adding reagents into the petri dish.

3. The pathogen resistance detection device according to claim 2, characterized in that, An electric telescopic rod is installed on the bottom inner wall of the detection box. The top end of the output rod of the electric telescopic rod is fixedly connected to the bottom of the vertical tube. A vertical rod is fixedly installed on the installation tube in the uppermost detection chamber. A connecting plate for fixing the liquid extraction tube is fixedly installed on the top end of the vertical rod.

4. The pathogen resistance detection device according to claim 1, characterized in that, The top of the fixed base and the bottom of the placement platform are both provided with matching annular grooves. Multiple balls are provided in the annular grooves of the fixed base to support the placement platform and ensure its stable rotation.

5. The pathogen resistance detection device according to claim 1, characterized in that, The top of the testing box is equipped with a first protective cover, the test tube is located inside the first protective cover, and a second protective cover and a third protective cover are respectively installed on both sides of the testing box. The driving mechanism and the linkage mechanism are located inside the second protective cover, and the heating mechanism is located inside the third protective cover.

6. The pathogen resistance detection device according to claim 1, characterized in that, Each detection chamber is equipped with a patch-type temperature sensor on its inner wall for monitoring the patch-type temperature sensor and for cooperating with the temperature controller to regulate the ambient temperature inside the three detection chambers.

7. The pathogen resistance detection device according to claim 1, characterized in that, Each detection chamber has a vent hole on its side wall and a check valve is installed on the vent hole. The check valve can prevent cold air from outside the detection chamber from entering the detection chamber.

8. The pathogen resistance detection device according to claim 1, characterized in that, Each of the detection chambers is equipped with a germicidal lamp on its inner wall to sterilize the environment inside the detection chamber before culturing pathogenic microorganisms.

9. The pathogen resistance detection device according to claim 1, characterized in that, Support frames are installed on the bottom of the partition and the top inner wall of the detection box. Wide-angle cameras are installed on the support frames to monitor the cultivation of pathogenic microorganisms in the petri dishes.

10. The pathogen resistance detection device according to claim 1, characterized in that, Each of the placement platforms is fixedly equipped with a handle for lifting the platform, so as to facilitate the simultaneous handling of multiple petri dishes or multiple test tubes.

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