Method of preventing and controlling fruit root knot with chitosan citrate as wound curing agent

Abstract

The present invention is method of preventing and controlling fruit root knot with chitosan citrate as wound curing agent, and relates to the recipe and preparation process of one new type of biological agricultural chemicals. The chitosan citrate wound curing agent is prepared through compounding water, citric acid, vitamin C and chitosan citrate in the weight ratio of 100 to (10-15) to (2-2.5) to (1-2) to prepare solution A; compounding water, potassium iodide, iodine and bioactive iodine compound in the weight ratio of 100 to (0.5-1) to (0.2-0.5) to (0.1-0.3) to prepare solution B; and adding solution B into the solution A to prepare the chitosan citrate wound curing agent. The chitosan citrate wound curing agent is paint onto the wound after scraping fruit root knot to reach the effect of curing fruit root knot.

Description

(1) Technical field

[0001] The invention relates to a formula for preventing and treating fruit tree root cancer by using a novel biopesticide chitosan oligosaccharide citrate callus agent and a preparation method thereof. (two), background technology

[0002] Fruit tree root cancer, also known as crown gall and root cancer, is a worldwide disease caused by Rhizobacillus soil. It has a wide parasitic range and can infect 643 species of plants belonging to 331 genera in 93 families. It invades plant cell tissues through wounds, causes chromosomal mutations, causes uncontrolled cell division, forms root nodules and seriously affects root growth. Root cancer mainly occurs in the root neck, but also in lateral roots and branch roots. It is more common in grafting sites. The symptoms are large, hard and lignified tumors. Due to the canceration of the roots, the circulation of water and nutrients is blocked, the roots decline day by day, the leaves are thin, thin, yellow in colo...

Examples

Embodiment 1

[0010] The technical scheme of the present embodiment is:

[0011] The first step: according to the ratio of water, citric acid, sodium hydroxide, vitamin C, chitosan oligosaccharide citrate weight ratio 100:10:5:2:1, citric acid, chitosan oligosaccharide citrate, vitamin C , Sodium hydroxide were poured into water and stirred in turn, dissolved until transparent, and it was A solution.

[0012] The second step: according to the weight ratio of water, potassium iodide, iodine, and biologically active organic iodine in the ratio of 100:0.5:0.2:0.1, pour potassium iodide, iodine, and biologically active organic iodine into water in sequence, stir while heating, and heat to 70 ℃, dissolve until transparent, and it is B solution.

[0013] Step 3: Slowly pour solution B into solution A under stirring, mix evenly until transparent, and become chitosan oligosaccharide citrate callus agent.

[0014] Field comparison test: In 2003, we selected 16 cherry tree saplings suffering from r...

Embodiment 2

[0016] The technical scheme of the present embodiment is:

[0017] The first step: mix citric acid, chitosan oligosaccharide citrate, vitamin C. Sodium hydroxide is poured into water successively and stirred until it dissolves until transparent, which is A solution.

[0018] Step 2: Put potassium iodide, iodine, and bioactive organic iodine into water in sequence according to the weight ratio of water, potassium iodide, iodine, and biologically active organic iodine in a weight ratio of 100:0.5:0.2:0.1, and heat while stirring to 70°C , dissolved until transparent, as B solution.

[0019] Step 3: Slowly pour the B solution into the A solution in the stirring state, mix evenly until it is transparent, and become a chitosan oligosaccharide citrate callus agent.

[0020] Field comparison test: In 2004, we selected a vineyard with a root cancer infection rate of 30% and divided it into two plots, each plot 666m2 2 , for the diseased trees in the test group, all the tumor-like d...

Embodiment 3

[0022] The technical scheme of the present embodiment is:

[0023] The first step: according to the ratio of water, citric acid, sodium hydroxide, vitamin C, chitosan oligosaccharide citrate weight ratio 100:10:4.5:2:1, citric acid, chitosan oligosaccharide citrate, vitamin C , Sodium hydroxide were poured into water and stirred in turn, dissolved until transparent, and it was A solution.

[0024] The second step: according to the weight ratio of water, potassium iodide, iodine, and bioactive organic iodine in the ratio of 100:0.5:0.2:0.1, potassium iodide, iodine, and bioactive organic iodine are placed in water in sequence, and heated while stirring to 70 ℃, dissolve until transparent, and it is B solution.

[0025] Step 3: Slowly pour the B solution into the A solution in the stirring state, mix evenly until it is transparent, and become a chitosan oligosaccharide citrate callus agent.

[0026] Field comparison test: In the spring of 2004, we selected the area where apple...

(1) Technical field

[0001] The invention relates to a formula for preventing and treating fruit tree root cancer by using a novel biopesticide chitosan oligosaccharide citrate callus agent and a preparation method thereof. (two), background technology

[0002] Fruit tree root cancer, also known as crown gall and root cancer, is a worldwide disease caused by Rhizobacillus soil. It has a wide parasitic range and can infect 643 species of plants belonging to 331 genera in 93 families. It invades plant cell tissues through wounds, causes chromosomal mutations, causes uncontrolled cell division, forms root nodules and seriously affects root growth. Root cancer mainly occurs in the root neck, but also in lateral roots and branch roots. It is more common in grafting sites. The symptoms are large, hard and lignified tumors. Due to the canceration of the roots, the circulation of water and nutrients is blocked, the roots decline day by day, the leaves are thin, thin, yellow in colo...

Examples

Embodiment 1

[0010] The technical scheme of the present embodiment is:

[0011] The first step: according to the ratio of water, citric acid, sodium hydroxide, vitamin C, chitosan oligosaccharide citrate weight ratio 100:10:5:2:1, citric acid, chitosan oligosaccharide citrate, vitamin C , Sodium hydroxide were poured into water and stirred in turn, dissolved until transparent, and it was A solution.

[0012] The second step: according to the weight ratio of water, potassium iodide, iodine, and biologically active organic iodine in the ratio of 100:0.5:0.2:0.1, pour potassium iodide, iodine, and biologically active organic iodine into water in sequence, stir while heating, and heat to 70 ℃, dissolve until transparent, and it is B solution.

[0013] Step 3: Slowly pour solution B into solution A under stirring, mix evenly until transparent, and become chitosan oligosaccharide citrate callus agent.

[0014] Field comparison test: In 2003, we selected 16 cherry tree saplings suffering from r...

Embodiment 2

[0016] The technical scheme of the present embodiment is:

[0017] The first step: mix citric acid, chitosan oligosaccharide citrate, vitamin C. Sodium hydroxide is poured into water successively and stirred until it dissolves until transparent, which is A solution.

[0018] Step 2: Put potassium iodide, iodine, and bioactive organic iodine into water in sequence according to the weight ratio of water, potassium iodide, iodine, and biologically active organic iodine in a weight ratio of 100:0.5:0.2:0.1, and heat while stirring to 70°C , dissolved until transparent, as B solution.

[0019] Step 3: Slowly pour the B solution into the A solution in the stirring state, mix evenly until it is transparent, and become a chitosan oligosaccharide citrate callus agent.

[0020] Field comparison test: In 2004, we selected a vineyard with a root cancer infection rate of 30% and divided it into two plots, each plot 666m2 2 , for the diseased trees in the test group, all the tumor-like d...

Embodiment 3

[0022] The technical scheme of the present embodiment is:

[0023] The first step: according to the ratio of water, citric acid, sodium hydroxide, vitamin C, chitosan oligosaccharide citrate weight ratio 100:10:4.5:2:1, citric acid, chitosan oligosaccharide citrate, vitamin C , Sodium hydroxide were poured into water and stirred in turn, dissolved until transparent, and it was A solution.

[0024] The second step: according to the weight ratio of water, potassium iodide, iodine, and bioactive organic iodine in the ratio of 100:0.5:0.2:0.1, potassium iodide, iodine, and bioactive organic iodine are placed in water in sequence, and heated while stirring to 70 ℃, dissolve until transparent, and it is B solution.

[0025] Step 3: Slowly pour the B solution into the A solution in the stirring state, mix evenly until it is transparent, and become a chitosan oligosaccharide citrate callus agent.

[0026] Field comparison test: In the spring of 2004, we selected the area where apple...