Calprotectin purification device

Abstract

The utility model relates to the technical field of purification devices, and particularly discloses a calprotectin purification device which comprises a purification tank body, a scraping assembly and a discharging assembly, the scraping assembly is used for cleaning calprotectin attached in an inner cavity of the purification tank body and is fixedly connected to the purification tank body, and the discharging assembly is used for discharging the calprotectin attached in the inner cavity of the purification tank body. The discharging assembly is used for overturning and discharging the purification tank body, the discharging assembly is fixedly connected to the scraping assembly, a scraping plate is machined to be in an inverted triangle shape, the scraping plate rotates to rapidly scrape off protein attached to the bottom of the purification tank body, and the protein is prevented from being attached to the bottom of the purification tank body; a threaded rod is used for adjusting the position of a scraper in the purification tank body, the threaded rod is used for adjusting the scraper to the bottom of the purification tank body, an annular plate is rotationally connected with a bearing frame through a pin shaft, so that the purification tank body is convenient to rotate, and protein in the purification tank body can be poured through overturning.

Description

Technical Field

[0001] The utility model relates to the technical field of purification devices, in particular to a calprotectin purification device. Background Art

[0002] The content of granulocyte calcium-binding protein in the human body is the basis for detecting acute inflammatory cell activation, and can also be used as the basis for detecting cell-specific or tissue-specific tests. Calprotectin can play a role in transmitting cell signals, as well as antimicrobial effects and immune regulation.

[0003] Among them, the content of calprotectin in normal human serum is very low, usually below 5ug per liter. The content of calprotectin in serum is higher than the normal range, which may be related to diseases such as inflammation, infection or tumor. When purifying calprotectin in serum, the serum sample is placed in a purification container, and a neutral salt solution is added to the purification container. The reaction is carried out under heating conditions, and the precipitated protein is placed in a reaction tank. The calprotectin is extracted by electrostatic effect and salt dissolution. When the solution in the purification container is heated, the protein particles precipitated in the serum sample are easily attached to the bottom of the purification container, which is not convenient for pouring out from the purification container for collection. For this reason, we propose a calprotectin purification device. Utility Model Content

[0004] The utility model aims to provide a calprotectin purification device to solve the problem in the above background technology that protein colloid particles precipitated from serum samples are easily attached to the bottom of the purification container and are not convenient to pour out from the purification container for collection.

[0005] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: a calprotectin purification device, comprising a purification tank, a scraper assembly, the scraper assembly is used to clean the calprotectin attached to the inner cavity of the purification tank, the scraper assembly is fixedly connected to the purification tank, and a discharge assembly is used to turn over the purification tank to discharge the material, and the discharge assembly is fixedly connected to the scraper assembly;

[0006] The scraper assembly comprises an annular plate, the annular plate is fixedly connected to the purification tank body, a carrier frame is rotatably connected to the annular plate, a threaded rod is threadedly connected in the carrier frame, and a scraper is fixedly connected to the threaded rod.

[0007] Preferably, a threaded sleeve is threadedly connected to the threaded rod, a sealing cover is fixedly connected to the threaded sleeve, and a saline conduit is fixedly connected to the sealing cover.

[0008] Preferably, a hand wheel is fixedly connected to the threaded rod.

[0009] Preferably, a circular ring is fixedly connected to the supporting frame, a supporting rod is sleeved in the circular ring, and a positioning pin is threadedly connected in the circular ring.

[0010] Preferably, a base is fixedly connected to the support rod.

[0011] Preferably, the discharge assembly includes a telescopic member, the telescopic member is fixedly connected to the carrier frame, a rack plate is fixedly connected to the telescopic member, a gear is meshedly connected to the rack plate, a rotating shaft is fixedly connected to the gear, and the rotating shaft is fixedly connected to the annular plate.

[0012] Preferably, a storage groove is machined in the base.

[0013] Preferably, a reaction tank is fixedly connected to the base, a high voltage generator is fixedly connected in the reaction tank, a discharge needle is arranged on the high voltage generator, a metal electrostatic coil is arranged in the reaction tank, and an end cover is sleeved on the reaction tank.

[0014] The utility model has at least the following beneficial effects:

[0015] The utility model can scrape and clean the calprotectin attached to the inner cavity of the purification tank body through the scraping component, and flip the purification tank body to discharge the material through the discharging component, so as to pour out the calprotectin in the purification tank body. In the scraping component, the scraper is processed into an inverted triangle shape, and the scraper can rotate to quickly scrape off the protein attached to the bottom of the purification tank body to prevent the protein from adhering to the bottom of the purification tank body. The use of the threaded rod can adjust the position of the scraper in the purification tank body, and the threaded rod is used to adjust the scraper to the bottom of the purification tank body. The annular plate is rotatably connected to the support frame by the pin shaft, so that the purification tank body can be rotated conveniently. The protein in the purification tank body can be dumped by flipping, and the annular plate drives the purification tank body to flip, so as to facilitate the discharge of the calprotectin purified in the purification tank body. Under the action of the telescopic member, the rack plate and the gear are used to transmit power, so that the flipping of the purification tank body is more convenient. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 It is a schematic diagram of the overall structure of the utility model.

[0017] Figure 2 It is a schematic structural diagram of the support frame, screw rod and scraper in the utility model.

[0018] Figure 3 It is an exploded schematic diagram of the telescopic member, rack plate and gear in the utility model.

[0019] Figure 4 It is a schematic diagram of the structure of the sealing cover, the threaded sleeve and the saline solution conduit in the utility model.

[0020] Figure 5 It is a structural schematic diagram of the support rod, base and storage slot in the utility model.

[0021] Figure 6 It is a structural schematic diagram of the reaction tank, high voltage generator and discharge needle in the utility model.

[0022] In the figure: 1-scraper assembly; 2-discharge assembly; 3-purification tank body; 4-annular plate; 5-carrying frame; 6-threaded rod; 7-scraper; 8-threaded sleeve; 9-sealing cover; 10-salt solution conduit; 11-handwheel; 12-circular ring; 13-support rod; 14-locating pin; 15-base; 16-storage slot; 17-telescopic part; 18-rack plate; 19-gear; 20-rotating shaft; 21-reaction tank; 22-high voltage generator; 23-discharge needle; 24-metal electrostatic circle; 25-end cover. DETAILED DESCRIPTION

[0023] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and understandable, the specific implementation methods of the present invention are described in detail below in conjunction with the accompanying drawings.

[0024] In the following description, many specific details are set forth to facilitate a full understanding of the present invention, but the present invention may also be implemented in other ways different from those described herein, and those skilled in the art may make similar generalizations without violating the connotation of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.

[0025] Secondly, the term "one embodiment" or "embodiment" as used herein refers to a specific feature, structure or characteristic that may be included in at least one implementation of the present invention. The term "in one embodiment" that appears in different places in this specification does not necessarily refer to the same embodiment, nor does it refer to a separate or selective embodiment that is mutually exclusive with other embodiments.

[0026] The content of calprotectin in normal human serum is very low, usually below 5ug per liter. The content of calprotectin in serum is higher than the normal range, which may be related to diseases such as inflammation, infection or tumor. When purifying calprotectin in serum, the serum sample is placed in a purification container, and a neutral salt solution is added to the purification container. The reaction is carried out under heating conditions, and the precipitated protein is placed in a reaction tank. The calprotectin is extracted by electrostatic effect and salt dissolution. When the solution in the purification container is heated, the protein particles precipitated in the serum sample are easily attached to the bottom of the purification container, which is not convenient for pouring out from the purification container for collection. For this reason, we propose a calprotectin purification device.

[0027] See also Figure 1-Figure 6The utility model provides a technical solution of a calprotectin purification device: a calprotectin purification device, comprising a purification tank body 3, a scraper assembly 1, the scraper assembly 1 is used to clean the calprotectin attached to the inner cavity of the purification tank body 3, the scraper assembly 1 is fixedly connected to the purification tank body 3, and a discharge assembly 2, the discharge assembly 2 is used to flip and discharge the purification tank body 3, and the discharge assembly 2 is fixedly connected to the scraper assembly 1.

[0028] The calprotectin purification device of the present embodiment can scrape and clean the calprotectin attached to the inner cavity of the purification tank body 3 through the scraper assembly 1, and flip the purification tank body 3 to discharge the material through the discharge assembly 2, so as to facilitate the pouring out of the calprotectin in the purification tank body 3. In the scraper assembly 1, the scraper 7 is processed into an inverted triangle shape, and the scraper 7 can quickly scrape off the protein attached to the bottom of the purification tank body 3 by rotation, so as to prevent the protein from adhering to the bottom of the purification tank body 3. The use of the threaded rod 6 can adjust the position of the scraper 7 in the purification tank body 3, and the threaded rod 6 is used to adjust the scraper 7 to the bottom of the purification tank body 3. The annular plate 4 is rotatably connected to the support frame 5 by the pin shaft, so as to facilitate the rotation of the purification tank body 3. The protein in the purification tank body 3 can be dumped by flipping, and the annular plate 4 drives the purification tank body 3 to flip, so as to facilitate the discharge of the calprotectin purified in the purification tank body 3. Under the action of the telescopic member 17, the rack plate 18 and the gear 19 are used to transmit power, so that the flipping of the purification tank body 3 is more convenient.

[0029] In some embodiments, the scraper assembly 1 includes an annular plate 4, which is fixedly connected to the purification tank 3. A support frame 5 is rotatably connected to the annular plate 4, a threaded rod 6 is threadedly connected to the support frame 5, and a scraper 7 is fixedly connected to the threaded rod 6. The serum solution containing calprotectin is poured into the purification tank 3. Under heating conditions, the serum solution and the neutral saline solution are mixed together, and the solubility of the protein in the saline solution increases. As the salt concentration continues to increase, the solubility of the protein decreases to varying degrees and precipitates successively. The high-concentration salt ions have a strong hydration force, which can capture the water of the protein molecules. The protein colloid particles are coagulated and precipitated in the purification tank body 3, and the threaded rod 6 in the support frame 5 is rotated. The threaded rod 6 drives the scraper 7 to extend to the bottom of the purification tank body 3. As the threaded rod 6 rotates, the scraper 7 scrapes off the protein deposited on the bottom of the purification tank body 3. In this embodiment, the scraper 7 is processed into an inverted triangle shape, and the scraper 7 can quickly scrape off the protein attached to the bottom of the purification tank body 3. The use of the threaded rod 6 can adjust the position of the scraper 7 in the purification tank body 3. The annular plate 4 is rotatably connected to the support frame 5 through a pin, which is convenient for exporting the protein in the purification tank body 3.

[0030] In some embodiments, a threaded sleeve 8 is threadedly connected to the threaded rod 6, a sealing cover 9 is fixedly connected to the threaded sleeve 8, a saline conduit 10 is fixedly connected in the sealing cover 9, the threaded sleeve 8 can be adjusted up and down on the threaded rod 6, the sealing cover 9 seals the purification tank body 3, and the setting of the saline conduit 10 facilitates the addition of neutral saline into the purification tank body 3. When the threaded rod 6 drives the scraper 7 to move to the top of the purification tank body 3, the threaded rod 6 is stopped from rotating, and the threaded sleeve 8 is rotated on the outer wall of the threaded rod 6 to drive the sealing cover 9 to move, and then the sealing cover 9 seals the top of the purification tank body 3.

[0031] In some embodiments, a handwheel 11 is fixedly connected to the threaded rod 6, and the handwheel 11 is configured to rotate the threaded rod 6. When the threaded rod 6 drives the scraper 7 to rotate to the bottom of the purification tank body 3, the threaded portion on the threaded rod 6 is separated from the supporting frame 5, and the threaded rod 6 can rotate freely in the supporting frame 5.

[0032] In some embodiments, a circular ring 12 is fixedly connected to the supporting frame 5, a support rod 13 is sleeved in the circular ring 12, a positioning pin 14 is threadedly connected in the circular ring 12, the circular ring 12 moves in the support rod 13 to drive the supporting frame 5 to move, the outer wall of the positioning pin 14 is processed with threads, and the positioning pin 14 is used to fix the position of the circular ring 12 on the support rod 13.

[0033] In some embodiments, a base 15 is fixedly connected to the support rod 13, and the base 15 supports the entire structure.

[0034] In some embodiments, the discharge assembly 2 includes a telescopic member 17, which is fixedly connected to the supporting frame 5. A rack plate 18 is fixedly connected to the telescopic member 17, and a gear 19 is meshed with the rack plate 18. A rotating shaft 20 is fixedly connected to the gear 19. The rotating shaft 20 is fixedly connected to the annular plate 4. The telescopic member 17 is an electric push rod. When the telescopic member 17 is extended, the rack plate 18 is driven to move, and the gear 19 is driven to rotate through the meshing between the rack plate 18 and the gear 19. The gear 19 drives the annular plate 4 to move through the rotating shaft 20, and then the purification tank body 3 is flipped. The annular plate 4 is driven to move through the rotating shaft 20, and the annular plate 4 drives the purification tank body 3 to flip, which facilitates the discharge of the calprotectin purified in the purification tank body 3. The rack plate 18 and the gear 19 are used to transmit power, making the flipping of the purification tank body 3 more convenient.

[0035] In some embodiments, a storage groove 16 is processed in the base 15, a reaction tank 21 is fixedly connected to the base 15, a high-voltage generator 22 is fixedly connected to the reaction tank 21, a discharge needle 23 is arranged on the high-voltage generator 22, a metal electrostatic ring 24 is arranged in the reaction tank 21, and an end cover 25 is sleeved on the reaction tank 21. The storage groove 16 is designed to place an alcohol lamp. The alcohol lamp is used as a heat source to more easily heat the purification tank body 3. After the solvent in the purification tank body 3 is heated, the ring 12 is adjusted on the outer wall of the support rod 13, and the ring 12 moves the purification tank body 3 to the inside of the reaction tank 21 through the supporting frame 5. Then, the high-voltage generator 22 is energized, and the discharge needle 23 generates an electrostatic effect, and then the electrostatic ions are guided out through the metal electrostatic ring 24 to enter the reaction tank 21. Because the electrostatic repulsion between the particles of the protein is the smallest in the electrostatic state, the solubility is the smallest, and the salt is dissolved to extract calprotectin. After completing this operation, calprotectin is deposited at the bottom of the purification tank body 3.

[0036] Importantly, it should be noted that the construction and arrangement of the present application shown in a plurality of different exemplary embodiments are only exemplary. Although only a few embodiments are described in detail in this disclosure, it should be readily understood by those who refer to this disclosure that many modifications are possible (e.g., the size, scale, structure, shape and ratio of various elements, and parameter values ​​(e.g., temperature, pressure, etc.), installation arrangements, use of materials, color, directional changes, etc.) without substantially departing from the novel teachings and advantages of the subject matter described in the application. For example, the element shown as integrally formed can be composed of multiple parts or elements, the position of the element can be inverted or otherwise changed, and the nature or number or position of the discrete element can be changed or changed. Therefore, all such modifications are intended to be included in the scope of the present utility model. The order or sequence of any process or method steps can be changed or reordered according to alternative embodiments. In the claims, any "device plus function" clause is intended to cover the structure of performing the function described herein, and is not only structurally equivalent but also equivalent structure. Without departing from the scope of the present invention, other substitutions, modifications, changes and omissions may be made in the design, operating conditions and arrangement of the exemplary embodiments. Therefore, the present invention is not limited to a specific embodiment, but extends to various modifications that still fall within the scope of the appended claims.

[0037] Additionally, in order to provide a concise description of example embodiments, all features of an actual embodiment (ie, those features that are not relevant to the best mode presently contemplated for carrying out the invention or those that are not relevant to implementing the invention) may not be described.

[0038] It will be appreciated that in the development of any actual implementation, as in any engineering or design project, numerous implementation-specific decisions may be made. Such a development effort may be complex and time-consuming, but will be a routine task of design, fabrication, and production for those of ordinary skill having the benefit of this disclosure without undue experimentation.

[0039] It should be noted that the above embodiments are only used to illustrate the technical solution of the utility model rather than to limit it. Although the utility model has been described in detail with reference to the preferred embodiments, ordinary technicians in the field should understand that the technical solution of the utility model can be modified or replaced by equivalents without departing from the spirit and scope of the technical solution of the utility model, which should be included in the scope of the claims of the utility model.

Claims

1. A calprotectin purification device, comprising a purification tank (3), Features: A scraper assembly (1), the scraper assembly (1) is used to clean the calprotectin attached to the inner cavity of the purification tank (3), and the scraper assembly (1) is fixedly connected to the purification tank (3); A discharge assembly (2), the discharge assembly (2) is used to turn over and discharge the purification tank (3), and the discharge assembly (2) is fixedly connected to the scraper assembly (1); The scraper assembly (1) comprises an annular plate (4), the annular plate (4) is fixedly connected to the purification tank body (3), a carrier frame (5) is rotatably connected to the annular plate (4), a threaded rod (6) is threadedly connected to the carrier frame (5), and a scraper (7) is fixedly connected to the threaded rod (6).

2. A calprotectin purification device according to claim 1, Features: A threaded sleeve (8) is threadedly connected to the threaded rod (6), a sealing cover (9) is fixedly connected to the threaded sleeve (8), and a saline conduit (10) is fixedly connected to the sealing cover (9).

3. A calprotectin purification device according to claim 1, Features: A hand wheel (11) is fixedly connected to the threaded rod (6).

4. A calprotectin purification device according to claim 1, Features: A circular ring (12) is fixedly connected to the carrier frame (5), a support rod (13) is sleeved in the circular ring (12), and a positioning pin (14) is threadedly connected in the circular ring (12).

5. A calprotectin purification device according to claim 4, Features: The support rod (13) is fixedly connected to a base (15).

6. A calprotectin purification device according to claim 1, Features: The discharge assembly (2) comprises a telescopic member (17), the telescopic member (17) and the carrier frame (5) are fixedly connected, a rack plate (18) is fixedly connected to the telescopic member (17), a gear (19) is meshedly connected to the rack plate (18), a rotating shaft (20) is fixedly connected to the gear (19), and the rotating shaft (20) and the annular plate (4) are fixedly connected.

7. A calprotectin purification device according to claim 5, Features: A storage groove (16) is formed in the base (15).

8. A calprotectin purification device according to claim 5, Features: A reaction tank (21) is fixedly connected to the base (15), a high voltage generator (22) is fixedly connected to the reaction tank (21), a discharge needle (23) is provided on the high voltage generator (22), a metal electrostatic ring (24) is provided in the reaction tank (21), and an end cover (25) is sleeved on the reaction tank (21).

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