Preservation device for anaerobic ammonium oxidation strains
By using a combination of storage container, drainage pipe and suspension parts in the storage device of anaerobic ammonia oxidized bacterial species, combined with temperature control device, the problem of loss of bacterial species during cleaning is solved, and the efficiency and stability of sewage treatment are improved sex.
Patent Information
- Application Number
- CN202422775027.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-11-14
- Publication Date
- 2024-12-10
- Estimated Expiration
- 2034-11-14
AI Technical Summary
Anaerobic ammonia oxidized bacterial species are easily discharged incorrectly during the cleaning process of the storage device, resulting in loss of bacterial species and affecting the efficiency of sewage treatment.
A storage device including a storage container, a drain pipe and a suspension piece is designed. By setting a limiting part and a stirrer in the tank body, the suction end is ensured to work in the supernatant, and the suspension part is fixedly connected to the suction end to adapt to The bacterial strain height should avoid the loss of bacterial strains and adjust the temperature through the temperature control device.
It effectively improves the cleaning efficiency, reduces the loss of anaerobic ammonia oxidizing bacteria, and ensures the stability and efficiency of sewage treatment.
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Figure CN222139091U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of sewage treatment, in particular to a storage device for anaerobic ammonia oxidizing bacteria. Background Art
[0002] As a biochemical technology, the anaerobic ammonium oxidation process uses anaerobic ammonium-oxidizing bacteria to convert ammonia nitrogen and nitrite into nitrogen gas, thereby achieving an efficient and energy-saving denitrification process.
[0003] However, due to fluctuations in wastewater quality characteristics, equipment maintenance, sudden equipment downtime caused by force majeure such as earthquakes, etc., these situations may cause the sewage treatment plant to temporarily stop production for weeks or even months. In addition, the proliferation rate of anaerobic ammonium oxidizing bacteria is slow and the environmental conditions are more demanding. Moreover, since anaerobic ammonium oxidizing bacteria are sensitive to temperature changes, a storage device for anaerobic ammonium oxidizing bacteria has been developed. The storage device is used to clean and store anaerobic ammonium oxidizing bacteria.
[0004] During the repeated cleaning process of the anaerobic ammonium oxidizing bacteria, the storage device needs to discharge the supernatant. However, due to the internal structure of the storage device, it is easy to mistakenly discharge the anaerobic ammonium oxidizing bacteria during the process of discharging the supernatant, resulting in the loss of the anaerobic ammonium oxidizing bacteria, which seriously affects the efficiency of wastewater treatment. Utility Model Content
[0005] In view of the shortcomings of the related technologies mentioned above, the present application provides a storage device for anaerobic ammonia-oxidizing bacteria to solve the above technical problems.
[0006] The present application provides a device for preserving anaerobic ammonia-oxidizing bacteria, comprising a storage container, a liquid discharge pipe and a suspension. The storage container is used to contain and clean the anaerobic ammonia-oxidizing bacteria. The liquid discharge pipe has a liquid suction end, which extends into the storage container and is movably arranged relative to the storage container. The liquid suction end is used to suck supernatant in the storage container. The suspension is fixedly connected to the liquid suction end, and the suspension is used to drive the liquid suction end to move so that the liquid suction end is in the supernatant.
[0007] In one embodiment of the present application, the storage container includes a tank body and an agitator, the tank body is used to contain anaerobic ammonia-oxidizing bacteria, the drain pipe is connected to the top of the tank body, the agitator is installed on the tank body, and the agitator is used to stir the anaerobic ammonia-oxidizing bacteria in the tank body.
[0008] In one embodiment of the present application, the drain pipe and the suspension are movably disposed in the gap between the agitator and the tank body.
[0009] In one embodiment of the present application, a limit member is disposed in the tank body, the limit member is located in the gap between the agitator and the tank body, and the limit member abuts against the drain pipe to limit the movement of the drain pipe toward the agitator.
[0010] In one embodiment of the present application, there are multiple limit members, which are spaced apart around the axis of the tank body and fixed to the tank wall of the tank body. In the circumferential direction of the tank body, the distance between the multiple limit members and the bottom of the tank body decreases successively.
[0011] In one embodiment of the present application, the agitator includes a motor and a stirring member. The motor is arranged outside the tank body and located on the top of the tank body. The output shaft of the motor is inserted into the tank body and connected to the stirring member. The stirring member is used to drive and stir the anaerobic ammonia-oxidizing bacteria in the tank body.
[0012] In one embodiment of the present application, the rotation axis of the agitator is colinear with the axis of the tank.
[0013] In one embodiment of the present application, the preservation device also includes a first temperature control device. A first insulation layer is provided on the outer surface of the tank body. The first temperature control device is connected to the first insulation layer. The temperature control device is used to adjust the temperature of the first medium and pump the first medium to the first insulation layer to control the temperature inside the tank body.
[0014] In one embodiment of the present application, the first heat-insulating layer includes a first pipe and a second pipe arranged in parallel, and the first pipe and the second pipe are spirally arranged around the axis of the tank body;
[0015] The first temperature control device includes a liquid outlet and a liquid inlet, the first pipe and the second pipe both include an upper port and a lower port that are far away from each other, the lower port is located on the side of the upper port close to the bottom of the tank body, the liquid inlet is connected to the upper port of the first pipe and the lower port of the second pipe, and the liquid outlet is connected to the lower port of the first pipe and the upper port of the second pipe.
[0016] In one embodiment of the present application, the storage device also includes a storage tank, which is selectively connected to or disconnected from the storage container, and the storage tank is used to preserve or transmit anaerobic ammonia-oxidizing bacteria.
[0017] In one embodiment of the present application, the preservation device also includes a second temperature control device, a second insulation layer is provided on the outer surface of the storage tank, the second temperature control device is connected to the second insulation layer, the temperature control device is used to adjust the temperature of the second medium and pump the second medium to the second insulation layer to control the temperature inside the storage tank.
[0018] The technical solution adopted by the utility model can achieve the following beneficial effects: the storage container can accommodate anaerobic ammonium oxidizing bacteria, and the anaerobic ammonium oxidizing bacteria can be cleaned and precipitated in the storage container. After a single cleaning and precipitation, the liquid suction end of the drain pipe can absorb the supernatant of the storage container. During this period, the suspension is fixed to the liquid suction end, and the suspension can adapt to the height of the anaerobic ammonium oxidizing bacteria in the tank. The suspension can avoid sucking out the anaerobic ammonium oxidizing bacteria, and can also enable the liquid suction end to discharge all the supernatant, which is beneficial to improve the cleaning efficiency of the storage device. BRIEF DESCRIPTION OF THE DRAWINGS
[0019] In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.
[0020] Figure 1 is a schematic structural diagram of a storage device shown in an exemplary embodiment of the present application;
[0021] Figure 2 is along Figure 1 The cross-sectional view of line AA in the figure;
[0022] Figure 3 is along Figure 1 The cross-sectional view of line BB in FIG.
[0023] Figure 4 is a schematic structural diagram of a storage device shown in another exemplary embodiment of the present application;
[0024] Figure 5 is along Figure 4 Cross-sectional view of line CC in;
[0025] Figure 6 is a structural schematic diagram of a storage device shown in yet another exemplary embodiment of the present application;
[0026] Figure 7 is along Figure 6 Cross-sectional view of line DD in FIG.
[0027] In the figure: 1, storage device; 100, storage container; 110, tank body; 111, injection port; 112, aeration pipe; 113, exhaust pipe; 114, active recovery water injection pipe; 115, cleaning pipe; 116, aeration structure; 117, pressure detection device; 118, temperature detection device; 119, guide part; 120, stirrer; 121, motor; 122, stirring member; 130, limit member; 140, first insulation layer; 141, first pipeline; 142, second pipeline; 150, accommodating chamber; 200, discharge pipe; 210, liquid suction end; 300, suspension member; 400, first temperature control device; 410, liquid outlet; 420, liquid inlet; 430, first pump body; 500, storage tank; 510, second insulation layer; 520, second pump body; 600, second temperature control device. DETAILED DESCRIPTION
[0028] The following will be combined with the drawings in the embodiments of the present application to clearly and completely describe the technical solutions in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, not all of the embodiments. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of this application.
[0029] It should be noted that all directional indications in the embodiments of the present application are only used to explain the relative position relationship, movement status, etc. between the components in a certain specific posture. If the specific posture changes, the directional indication will also change accordingly.
[0030] In this application, unless otherwise clearly specified and limited, the terms "connection", "fixation", etc. should be understood in a broad sense. For example, "fixation" can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements, unless otherwise clearly defined. For ordinary technicians in this field, the specific meanings of the above terms in this application can be understood according to specific circumstances.
[0031] As a biochemical technology, the anaerobic ammonium oxidation process uses anaerobic ammonium-oxidizing bacteria to convert ammonia nitrogen and nitrite into nitrogen gas, thereby achieving an efficient and energy-saving denitrification process.
[0032] However, due to fluctuations in wastewater quality characteristics, equipment maintenance, sudden equipment downtime caused by force majeure such as earthquakes, etc., these situations may cause the sewage treatment plant to temporarily stop production for weeks or even months. In addition, the proliferation rate of anaerobic ammonium oxidizing bacteria is slow and the environmental conditions are relatively harsh. Moreover, since anaerobic ammonium oxidizing bacteria are sensitive to temperature changes, a storage device for anaerobic ammonium oxidizing bacteria has been developed. The storage device is used to clean and preserve anaerobic ammonium oxidizing bacteria.
[0033] During the repeated cleaning process of the anaerobic ammonium oxidizing bacteria, the storage device needs to discharge the supernatant. However, due to the internal structure of the storage device, it is easy to mistakenly discharge the anaerobic ammonium oxidizing bacteria during the process of discharging the supernatant, resulting in the loss of the anaerobic ammonium oxidizing bacteria, which seriously affects the efficiency of wastewater treatment.
[0034] This embodiment provides a storage device 1 for anaerobic ammonia-oxidizing bacteria. Figure 1 as well as Figure 2 The storage device 1 is referred to as the storage device 1 in the following. The storage device 1 may include a storage container 100 , a liquid discharge pipe 200 , and a suspension member 300 . The storage container 100 accommodates the liquid discharge pipe 200 and the suspension member 300 . The suspension member 300 is fixed to the liquid discharge pipe 200 .
[0035] The storage container 100 is used to contain and clean the anaerobic ammonium oxidizing bacteria. The storage container 100 may include a tank body 110 and an agitator 120. The tank body 110 may be roughly a cylindrical structure, a truncated cone structure, etc. The tank body 110 may have a receiving cavity 150, and the receiving cavity 150 is used to contain the anaerobic ammonium oxidizing bacteria. The anaerobic ammonium oxidizing bacteria can be cleaned, preserved, and restored to activity in the tank body 110. The drain pipe 200 is connected to the top of the tank body 110. This arrangement can prevent the anaerobic ammonium oxidizing bacteria from leaking from the gap between the drain pipe 200 and the tank body 110, thereby improving the use effect of the storage device 1.
[0036] In this embodiment, Anammox sludge taken from an anaerobic ammonium oxidation (Anammox) fixed bed reactor (FBR) is used as the anaerobic ammonium oxidizing bacteria strain for preservation, which is beneficial for the rapid start-up of the Anammox process.
[0037] For more details, please refer to Figure 2 The tank body 110 may also include a material injection port 111, an aeration pipe 112, an exhaust pipe 113, an activity recovery water injection pipe 114, and a cleaning pipe 115. It is understandable that the material injection port 111 can discharge the anaerobic ammonia oxidizing bacteria into the tank body 110, and the material injection port 111 can also discharge the anaerobic ammonia oxidizing bacteria after activity recovery or cleaning out of the tank body 110. The tank body 110 may also include an aeration structure 116, which is arranged in the accommodating chamber 150 and is connected to the aeration pipe 112. The aeration pipe 112 is used to discharge inert gas, such as argon, etc., and the aeration structure 116 is used to discharge and stir the anaerobic ammonia oxidizing bacteria and cleaning agents inside the tank body 110, so that the tank body 110 has an atmosphere for storing and recovering the activity of the anaerobic ammonia oxidizing bacteria. The exhaust pipe 113 can discharge gas. The activity recovery water injection pipe 114 can be used to inject an activity recovery agent, etc. into the tank body 110. The cleaning pipe 115 can inject a cleaning medium, such as oxygen-free water, into the tank body 110. This arrangement allows the tank body 110 to not only clean and store anaerobic ammonia-oxidizing bacteria, but also restore the activity of anaerobic ammonia-oxidizing bacteria. The storage container 100 can take into account multiple functions, reduce the number of other devices, reduce the volume and operation difficulty of the storage device 1, and improve the application prospects of the storage device 1.
[0038] In addition, please continue to see Figure 2The tank body 110 may also include a pressure detection device 117 and a temperature detection device 118. The pressure detection device 117 is used to detect the internal pressure of the tank body 110, and the temperature detection device 118 is used to detect the internal temperature of the tank body 110. This arrangement can monitor the pressure and temperature of the tank body 110 to avoid the extinction of the activity of the anaerobic ammonia-oxidizing bacteria due to inappropriate pressure or temperature of the tank body 110.
[0039] Please continue reading Figure 2 The agitator 120 is installed on the tank body 110, and is used to agitate the anaerobic ammonium oxidizing bacteria in the tank body 110. In the subsequent activity recovery process of the anaerobic ammonium oxidizing bacteria, the agitator 120 can agitate the anaerobic ammonium oxidizing bacteria at the bottom to the top, and make the anaerobic ammonium oxidizing bacteria more mixed and more uniform.
[0040] In one embodiment, the agitator 120 may include a motor 121 and a stirring member 122, wherein the motor 121 is disposed outside the tank body 110 and located at the top of the tank body 110, and the output shaft of the motor 121 is inserted into the tank body 110 and connected to the stirring member 122. The stirring member 122 is used to drive and stir the anaerobic ammonia-oxidizing bacteria in the tank body 110, and this arrangement can prevent the motor 121 from short-circuiting due to water ingress, thereby improving the safety of the motor 121.
[0041] Preferably, the rotation axis of the stirrer 120 is colinear with the axis of the tank body 110. Exemplarily, the tank body 110 may be in the shape of a cylinder, and the rotation axis of the stirrer 120 may be colinear with the axis of the cylinder, and then the distance from the rotation axis to the inner wall of the tank body 110 is the same. This arrangement can increase the stirring range of the stirrer 120 to improve its stirring effect.
[0042] In this example, see Figure 2 as well as Figure 3 The drain pipe 200 may be a generally tubular structure, that is, it may be a hollow structure. The drain pipe 200 may have a liquid suction end 210, and the liquid suction end 210 is used to suck the supernatant in the storage container 100. The liquid suction end 210 extends into the storage container 100, and the liquid suction end 210 is movably arranged relative to the storage container 100. In other words, the drain pipe 200 can extend into or out of the storage container 100, so that the washing liquid end is movably arranged relative to the storage container 100.
[0043] In one embodiment, see Figure 3, the length of the portion of the drain pipe 200 located in the tank body 110 is too long, and it may be entangled with the agitator 120, thereby affecting the normal operation of the drain pipe 200. In this embodiment, a limiter 130 may be provided in the tank body 110, and the limiter 130 is located in the gap between the agitator 120 and the tank body 110. The limiter 130 abuts against the drain pipe 200 to limit the movement of the drain pipe 200 toward the agitator 120. The limiter 130 can limit the movement of the drain pipe 200 toward the agitator 120, thereby improving the safety of the storage device 1, but it will not affect the up and down movement of the drain pipe 200, thereby ensuring that the drain pipe 200 has a normal liquid absorption effect.
[0044] Specifically, the stopper 130 can be a roughly rod-shaped structure, and its extension direction is the top-down direction of the tank body 110. There are multiple stoppers 130, and multiple stoppers 130 are located in the accommodating chamber 150. Multiple stoppers 130 are spaced around the axis of the tank body 110. In other words, multiple stoppers 130 are distributed along the circumference of the tank body 110, and multiple stoppers 130 are spaced apart from each other. Multiple stoppers 130 are all fixed to the tank wall of the tank body 110, and in the circumference of the tank body 110, the distance between the multiple stoppers 130 and the bottom of the tank body 110 decreases successively. Furthermore, the distance between the liquid suction end 210 and the bottom will also gradually decrease, so that the liquid suction end 210 can adapt to the supernatant of different liquid level heights to maintain the normal function of the discharge pipe 200.
[0045] Also, please continue reading Figure 3 The tank body 110 may also be provided with a guide portion 119, which is arranged inside the tank body 110 and is distributed in a spiral shape. The guide portion 119 is used to guide the movement of the liquid suction end 210 to prevent it from moving toward the stirrer 120, thereby ensuring the normal operation of the stirrer 120.
[0046] In this example, please continue to refer to Figure 3 The material of the suspension member 300 can be foam plastic or polypropylene, etc., and this embodiment is not limited, that is, the suspension member 300 can be suspended on the surface of the supernatant. The suspension member 300 is fixedly connected to the liquid suction end 210, and the suspension member 300 is used to drive the liquid suction end 210 to move so that the liquid suction end 210 is in the supernatant. The suspension can provide buoyancy to the liquid suction end 210, so that the liquid suction end 210 is always in the supernatant. The suspension member 300 can adapt to the height of the anaerobic ammonia-oxidizing bacteria in the tank body 110. The suspension member 300 can avoid sucking out the anaerobic ammonia-oxidizing bacteria, and can also enable the liquid suction end 210 to discharge all the supernatant, which is beneficial to improve the cleaning efficiency of the storage device 1.
[0047] Preferably, in order to improve the safety of the suspension member 300 and the drainage pipe 200, the drainage pipe 200 and the suspension member 300 are movably arranged in the gap between the agitator 120 and the tank body 110 through devices such as the stopper 130. This arrangement can prevent the drainage pipe 200 and its liquid suction end 210 from hindering the normal operation of the agitator 120, thereby improving its safety.
[0048] In this example, see Figure 4 as well as Figure 5 The storage device 1 may further include a first temperature control device 400, which may be a plate heat exchanger or a cooling tower, etc., which is not limited in this embodiment. A first insulation layer 140 is provided on the outer surface of the tank body 110, and the first insulation layer 140 may exchange heat with the tank body 110. The first temperature control device 400 is connected to the first insulation layer 140, and the first temperature control device 400 is used to adjust the temperature of the first medium and pump the first medium to the first insulation layer 140 to control the temperature inside the tank body 110. The preservation and activity recovery of anaerobic ammonia-oxidizing bacteria require temperature control, and the first temperature control device 400 controls the temperature of the tank body 110 to ensure that the anaerobic ammonia-oxidizing bacteria are in the correct temperature environment, thereby effectively reducing the loss of anaerobic ammonia-oxidizing bacteria.
[0049] Please continue reading Figure 5 The first heat-insulating layer 140 may include a first pipe 141 and a second pipe 142, which are arranged in parallel and spirally arranged around the axis of the tank body 110. They are not only parallel to each other, but also closely surround the axis of the tank body 110 and stretch in a spiral shape. This layout not only ensures that the pipes can cover the surface of the tank body 110 to the maximum extent, thereby effectively improving the heat-insulating effect, but also makes the entire structure look more compact and beautiful.
[0050] More specifically, the first temperature control device 400 may include a liquid outlet 410 and a liquid inlet 420. For the first temperature control device 400, the first medium is discharged from the liquid outlet 410 and then flows back through the liquid inlet 420. In addition, the first temperature control device 400 may include a first pump body 430, which may be connected to the liquid outlet 410 and used to provide power for the first medium. The first pipe 141 and the second pipe 142 both include an upper port and a lower port that are away from each other, and the lower port is located on the side of the upper port close to the bottom of the tank body 110. The liquid inlet 420 is connected to the upper port of the first pipe 141 and the lower port of the second pipe 142, and the liquid outlet 410 is connected to the lower port of the first pipe 141 and the upper port of the second pipe 142. It can be understood that in the first pipe 141, the first medium flows into the first pipe 141 from the lower port of the first pipe 141, and then flows out of the first pipe 141 through the upper port of the first pipe 141, and the cycle repeats. In the second pipe 142, the first medium flows into the second pipe 142 from the upper port of the second pipe 142, and then flows out of the first pipe 141 through the lower port of the second pipe 142, and the cycle repeats. This arrangement not only ensures that the liquid can form a complex circulation flow in the pipe, but also makes the temperature control of the entire insulation system more accurate and efficient.
[0051] Due to the large number of anaerobic ammonium oxidizing bacteria, they cannot be completely stored in the tank 110. Figure 6 The storage device 1 may further include a storage tank 500. The storage tank 500 may be in the shape of a cylinder or a truncated cone, etc., which is not limited in this embodiment. The storage tank 500 selectively connects or disconnects the storage container 100. The storage tank 500 is used to store or transmit anaerobic ammonia-oxidizing bacteria to solve the problem that the tank body 110 cannot completely store the anaerobic ammonia-oxidizing bacteria.
[0052] For example, see Figure 6 as well as Figure 7When the anaerobic ammonium oxidizing bacteria need to be preserved due to an emergency, the anaerobic ammonium oxidizing bacteria can be discharged into the tank body 110 for cleaning and preservation. However, when more anaerobic ammonium oxidizing bacteria need to be cleaned and stored later, the storage tank 500 can be connected to the tank body 110 of the storage container 100, and a second pump body 520 is provided between the storage tank 500 and the tank body 110. After the anaerobic ammonium oxidizing bacteria in the tank body 110 are cleaned, the second pump body 520 can discharge the anaerobic ammonium oxidizing bacteria in the tank body 110 into the storage tank 500, and the storage tank 500 can improve the storage environment of the anaerobic ammonium oxidizing bacteria to achieve the storage of the anaerobic ammonium oxidizing bacteria. When the anaerobic ammonium oxidizing bacteria are needed later, the storage tank 500 can re-transfer a certain volume of anaerobic ammonium oxidizing bacteria to the tank body 110, and use the tank body 110 to restore the activity of the anaerobic ammonium oxidizing bacteria for subsequent use. The provision of the storage tank 500 can reduce the volume of the tank body 110 , and flexibly adjust the number of anaerobic ammonia-oxidizing bacteria to be restored in the tank body 110 , thereby improving the use effect of the storage device 1 .
[0053] Please continue reading Figure 6 as well as Figure 7 The storage device 1 may further include a second temperature control device 600. The outer surface of the storage tank 500 is provided with a second insulation layer 510. The second temperature control device 600 is connected to the second insulation layer 510. The second temperature control device 600 is used to adjust the temperature of the second medium and pump the second medium to the second insulation layer 510 to control the temperature in the storage tank 500. The outer surface of the storage tank 500 is provided with a second insulation layer 510. The second insulation layer 510 can exchange heat with the storage tank 500. The second temperature control device 600 is connected to the second insulation layer 510. The second temperature control device 600 is used to adjust the temperature of the second medium and pump the second medium to the second insulation layer 510 to control the temperature in the storage tank 500. The storage of anaerobic ammonia oxidizing bacteria requires strict temperature control. The second temperature control device 600 controls the temperature of the storage tank 500 to ensure that the anaerobic ammonia oxidizing bacteria are in the correct temperature environment, effectively reducing the loss of anaerobic ammonia oxidizing bacteria.
[0054] In addition, the second insulation layer 510 may also have two parallel pipes, and the two pipes are arranged in parallel around the storage tank 500. This arrangement can effectively improve the insulation effect and make the entire structure look more compact and beautiful. In addition, the second medium can have different flow directions in the two pipes to make the temperature control of the entire insulation system more accurate and efficient, which will not be repeated here.
[0055] For ease of understanding, the following content exemplarily introduces the method of using the storage device 1:
[0056] (1) Clean the anaerobic ammonia-oxidizing bacteria that need to be stored. Inject the anaerobic ammonia-oxidizing bacteria that need to be stored into the tank body 110 from the injection port 111, and place the anaerobic ammonia-oxidizing bacteria in the tank body 110 for natural sedimentation for 30 minutes. In addition, in this embodiment, anaerobic water without matrix can be added through the cleaning pipe 115 to wash in the storage container 100, and then naturally precipitate for 30 minutes. The anaerobic water without matrix is composed of nutrient salt solution, inorganic salt solution and tap water, etc., and argon gas is flushed into it through the aeration structure 116. During this period, after each cleaning, the supernatant in the container can be siphoned through the drain pipe 200 so that the supernatant is completely discharged from the container to complete the cleaning of the anaerobic ammonia-oxidizing bacteria.
[0057] (2) Storing anaerobic ammonia-oxidizing bacteria. In this embodiment, argon gas can be filled into the sealed storage container 100 through the aeration structure 116. For example, by injecting high-purity argon gas into the tank body 110, the pressure increase rate in the tank is controlled at 0.01MPa / min-0.02MP / min, and the pressure in the bacteria storage container 100 finally reaches 0.2MPa-0.25MPa, and the sealing condition of the bacteria storage container 100 is checked. In addition, the storage temperature is selected according to the intended storage period. For example, during the short-term storage of anaerobic ammonia-oxidizing bacteria for 1 day to 30 days, the first temperature control device 400 is used to control the first insulation layer 140 so that the temperature condition in the tank body 110 is room temperature of 20±4°C.
[0058] In addition, when the number of anaerobic ammonium oxidizing bacteria is large and the tank body 110 cannot store them completely, the tank body 110 of this embodiment can discharge the cleaned anaerobic ammonium oxidizing bacteria into the storage tank 500. The storage tank 500 can store a large number of anaerobic ammonium oxidizing bacteria, and it can also inject high-purity argon gas to adjust the internal pressure, and adjust the temperature of the storage tank 500 through the second control device. The storage tank 500 can also have a storage environment for anaerobic ammonium oxidizing bacteria.
[0059] (3) Activity recovery of anaerobic ammonia-oxidizing bacteria after storage. The pressure in the storage container 100 is slowly reduced to normal pressure through the exhaust pipe 113, etc. Among them, the speed of controlling the exhaust pipe 113 to reduce the pressure in the storage container 100 can be 0.005MPa / min-0.01MPa / min. After decompression, high-purity argon gas is introduced into the storage container 100 through the aeration structure 116, and the agitator 120 is synchronously controlled to stir, and the first temperature control device 400 is used to control the first insulation layer 140 to heat up, thereby providing heat for the high-purity argon gas. For example, the heating rate is 1°C / h-2°C / h, and the temperature is finally controlled at about 30°C and left to stand for 4h-6h. The anaerobic ammonia-oxidizing bacteria after heating can also be washed with oxygen-free water without matrix. Argon is introduced through the aeration structure 116 and stirred for 10 minutes. After standing for 1-2 hours, the supernatant in the container is sucked out by siphoning through the drain pipe 200 so that the supernatant is completely discharged from the container. The washing can remove the residual matrix, metabolites and dead and disintegrated anaerobic ammonia-oxidizing bacteria on the surface of the anaerobic ammonia-oxidizing bacteria. In addition, the anaerobic ammonia-oxidizing bacteria to be restored in activity are taken out through the injection port 111 or other positions at the bottom of the storage container 100 for subsequent sewage treatment.
[0060] In addition, when only a portion of the anaerobic ammonium oxidizing bacteria are restored to activity, the tank body 110 can discharge the anaerobic ammonium oxidizing bacteria that do not need to be restored to activity into the storage tank 500. The storage tank 500 can flexibly adjust the number of anaerobic ammonium oxidizing bacteria to be restored to activity in the tank body 110, so as to avoid the anaerobic ammonium oxidizing bacteria that do not need to be restored to activity being restored, reduce energy waste, and improve the use effect of the storage device 1.
[0061] This embodiment provides a storage device 1 for anaerobic ammonium oxidizing bacteria, and the storage container 100 can accommodate anaerobic ammonium oxidizing bacteria, and the anaerobic ammonium oxidizing bacteria can be cleaned and precipitated in the storage container 100. After a single cleaning and precipitation, the liquid suction end 210 of the drain pipe can suck the supernatant of the storage container 100. During this period, the suspension 300 is fixed to the liquid suction end 210, and the suspension 300 can adapt to the height of the anaerobic ammonium oxidizing bacteria in the tank body 110. The suspension 300 can avoid sucking out the anaerobic ammonium oxidizing bacteria, and can also enable the liquid suction end 210 to discharge all the supernatant, which is beneficial to improve the cleaning efficiency of the storage device 1.
[0062] In the embodiments of the present application, the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance. "Including" and "comprising" mentioned throughout the specification and claims are open-ended terms and should be interpreted as "including but not limited to".
[0063] The above embodiments are merely illustrative of the principles and effects of the present application, and are not intended to limit the present application. Anyone familiar with the technology may modify or change the above embodiments without violating the spirit and scope of the present application. Therefore, all equivalent modifications or changes made by a person of ordinary skill in the art without departing from the spirit and technical ideas disclosed in the present application shall still be covered by the claims of the present application.
Claims
1. A storage device for anaerobic ammonium-oxidizing bacteria, characterized in that: include: A storage container, wherein the storage container is used to contain and clean the anaerobic ammonium-oxidizing bacteria; A liquid discharge pipe, the liquid discharge pipe having a liquid suction end, the liquid suction end extending into the storage container and movably arranged relative to the storage container, the liquid suction end being used to suck the supernatant in the storage container; A suspension member is fixedly connected to the liquid aspirating end and is used to drive the liquid aspirating end to move so that the liquid aspirating end is in the supernatant.
2. The storage device according to claim 1, characterized in that The storage container comprises a tank body and a stirrer, wherein the tank body is used to contain the anaerobic ammonium-oxidizing bacteria, the drain pipe is connected to the top of the tank body, the stirrer is installed on the tank body, and the stirrer is used to stir the anaerobic ammonium-oxidizing bacteria in the tank body.
3. The storage device according to claim 2, characterized in that The liquid discharge pipe and the suspension member are movably arranged in the gap between the agitator and the tank body.
4. The storage device according to claim 3, characterized in that A limiting member is arranged in the tank body, and the limiting member is located in the gap between the agitator and the tank body. The limiting member abuts against the drain pipe to limit the movement of the drain pipe toward the agitator.
5. The storage device according to claim 4, characterized in that There are multiple limiters, which are distributed at intervals around the axis of the tank body and fixed to the tank wall of the tank body. In the circumferential direction of the tank body, the distances between the multiple limiters and the bottom of the tank body decrease successively.
6. The storage device according to claim 2, characterized in that: The agitator comprises a motor and a stirring member, wherein the motor is arranged outside the tank body and located at the top of the tank body, the output shaft of the motor is inserted into the tank body and connected to the stirring member, and the stirring member is used to be driven to stir the anaerobic ammonia-oxidizing bacteria in the tank body; And / or, the rotation axis of the agitator is colinear with the axis of the tank body.
7. The storage device according to claim 2, characterized in that: The storage device also includes a first temperature control device. The outer surface of the tank body is provided with a first insulation layer. The first temperature control device is connected to the first insulation layer. The temperature control device is used to adjust the temperature of the first medium and pump the first medium to the first insulation layer to control the temperature inside the tank body.
8. The storage device according to claim 7, characterized in that The first heat-insulating layer comprises a first pipe and a second pipe arranged in parallel, and the first pipe and the second pipe are spirally arranged around the axis of the tank body; The first temperature control device includes a liquid outlet and a liquid inlet, the first pipe and the second pipe each include an upper port and a lower port that are far away from each other, the lower port is located on the side of the upper port close to the bottom of the tank body, the liquid inlet is connected to the upper port of the first pipe and the lower port of the second pipe, and the liquid outlet is connected to the lower port of the first pipe and the upper port of the second pipe.
9. The storage device according to any one of claims 1 to 8, characterized in that: The storage device also includes a storage tank, which is selectively connected to or disconnected from the storage container, and is used to preserve or transport the anaerobic ammonia-oxidizing bacteria.
10. The storage device according to claim 9, characterized in that The storage device also includes a second temperature control device. The outer surface of the storage tank is provided with a second insulation layer. The second temperature control device is connected to the second insulation layer. The temperature control device is used to adjust the temperature of the second medium and pump the second medium to the second insulation layer to control the temperature inside the storage tank.
