Multi-concave-surface cell culture sheet

Abstract

The utility model provides a multi-concave-surface cell culture sheet, and relates to the technical field of cell culture. The multi-concave-surface cell culture sheet comprises a culture sheet body and at least one clamping part, a plurality of concave surfaces sunken downwards are arranged on the top surface of the culture sheet body, the concave surfaces are used for culturing cell balls, the clamping part is of an inverted convex structure, each clamping part is arranged to protrude upwards from the top surface of the culture sheet body, and the clamping part comprises a connecting part and a limiting part which are connected; the two ends of the connecting part are used for connecting the culture sheet body and the limiting part, and the limiting part protrudes out of the side face of the connecting part, so that the limiting part forms a limiting face facing downwards, the limiting effect on tweezers for clamping the connecting part is achieved, and the multi-concave-surface cell culture sheet is prevented from falling off when being clamped.

Description

Technical Field

[0001] The utility model relates to the technical field of cell culture, in particular to a multi-concave cell culture sheet. Background Art

[0002] Three-dimensional cell culture technology refers to the co-culturing of carriers with different materials with three-dimensional structures and various types of cells in vitro, so that the cells can migrate and grow in the three-dimensional spatial structure of the carrier, forming a three-dimensional cell-carrier complex. That is, three-dimensional cell culture technology can simulate the in vivo environment to the greatest extent, and can also show the advantages of intuitiveness and controllable conditions of cell culture. In the prior art, three-dimensional cell spheroid multi-concave culture sheets can not only achieve the above-mentioned culture effects, but also have the characteristics of easy operation, simple pretreatment methods and low cost.

[0003] However, since the three-dimensional cell spheroid multi-concave culture sheet usually needs to be placed in the culture medium in the culture container for cell culture, the friction of the clamping part will decrease due to the adhesion problem of the culture medium. When removing it, it may cause the culture sheet to fall off accidentally, resulting in the loss of experimental materials. Utility Model Content

[0004] One purpose of the utility model is to provide a multi-concave cell culture sheet to solve the technical problem in the prior art that the multi-concave cell culture sheet is easy to fall off when being removed.

[0005] Another purpose of the utility model is to improve the anti-slip performance of the clamping part in the multi-concave cell culture sheet.

[0006] In particular, the utility model provides a multi-concave cell culture sheet, comprising:

[0007] The culture sheet body has a plurality of concave surfaces sunken downwards on its top surface, and the concave surfaces are used for culturing cell spheres;

[0008] At least one clamping portion, the clamping portion is an inverted convex structure, each clamping portion is configured to protrude upward from the top surface of the culture sheet body, the clamping portion includes a connected connecting portion and a limiting portion, the two ends of the connecting portion are used to connect the culture sheet body and the limiting portion, the limiting portion protrudes from the side of the connecting portion so that the limiting portion forms a limiting surface facing downward.

[0009] Optionally, a groove is provided on the peripheral side of one end of the connecting portion close to the limiting portion.

[0010] Optionally, a non-slip rubber sleeve is provided on the surface of the connecting portion.

[0011] Optionally, the groove surface is provided with anti-slip features.

[0012] Optionally, an external thread is provided on the outer peripheral surface of the upper part of the connecting part, and the limiting part is provided with a threaded hole that forms a threaded connection with the external thread. After the limiting part is connected to the connecting part, at least part of the external thread is exposed at the bottom of the limiting part, and the external thread exposed at the bottom of the limiting part is used to form the anti-slip feature.

[0013] Optionally, the limiting portion is cylindrical and the connecting portion is cylindrical.

[0014] Optionally, the number of the clamping part is one, and the clamping part is located at the center of the culture sheet body.

[0015] Optionally, it also includes:

[0016] The convex ring protrudes upward from the edge of the top surface of the culture sheet body, so that the convex ring and the surface of the culture sheet body are surrounded to form a receiving space.

[0017] Optionally, the concave surface has a preset height and a bottom thereof is configured in a spherical shape.

[0018] Optionally, the multi-concave culture sheet is an integral injection-molded structure.

[0019] The center of the multi-concave cell culture sheet of the utility model is provided with a clamping part having a connecting part and a limiting part, and the two ends of the connecting part are used to connect the culture sheet body and the limiting part, and the limiting part protrudes from the side of the connecting part so that the limiting part forms a limiting surface facing downward. In the above technical scheme, the limiting part is arranged above the connecting part and protrudes from the side of the connecting part, so that the limiting part forms a limiting surface facing downward, so as to limit the tweezers clamped on the connecting part, so as to prevent the multi-concave cell culture sheet from falling due to insufficient friction on the surface of the connecting part when being clamped by the tweezers, thereby avoiding the loss of experimental materials.

[0020] Furthermore, the groove surface of the connection part of the utility model is provided with anti-slip features to improve the anti-slip performance of the connection part.

[0021] The above description is only an overview of the technical solution of the utility model. In order to more clearly understand the technical means of the utility model and implement it according to the contents of the specification, the following is a detailed description of the preferred embodiments of the utility model in conjunction with the accompanying drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Hereinafter, some specific embodiments of the present invention will be described in detail in an exemplary and non-limiting manner with reference to the accompanying drawings. The same reference numerals in the accompanying drawings indicate the same or similar components or parts. It should be understood by those skilled in the art that these drawings are not necessarily drawn to scale. In the accompanying drawings:

[0023] Figure 1is a schematic structural diagram of a multi-concave cell culture sheet according to an embodiment of the utility model;

[0024] Figure 2 is along Figure 1 A schematic cross-sectional view taken along the cutting line AA in FIG.

[0025] Figure 3 A schematic structural diagram of a clamping portion according to an embodiment of the utility model;

[0026] Figure 4 is a schematic structural diagram of a clamping portion according to another embodiment of the utility model;

[0027] Figure 5 It is a schematic structural diagram of a clamping portion according to another embodiment of the utility model.

[0028] Figure numerals: 100 - multi-concave cell culture sheet, 200 - anti-slip rubber sleeve, 10 - culture sheet body, 11 - concave surface, 20 - clamping part, 21 - connecting part, 221 - groove, 22 - limiting part, 221 - limiting surface, 40 - convex ring. DETAILED DESCRIPTION

[0029] In order to make the above-mentioned purposes, features and advantages of the present application more obvious and easy to understand, the specific implementation methods of the present application are described in detail below in conjunction with the accompanying drawings. It is to be understood that the specific embodiments described herein are only used to explain the present application, rather than to limit the present application. It should also be noted that, for ease of description, only some structures related to the present application are shown in the accompanying drawings, rather than all structures. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of this application.

[0030] The terms "including" and "having" and any variations thereof in this application are intended to cover non-exclusive inclusions. For example, a process, method, system, product or device comprising a series of steps or units is not limited to the listed steps or units, but may optionally include steps or units not listed, or may optionally include other steps or units inherent to these processes, methods, products or devices.

[0031] Reference to "embodiments" herein means that a particular feature, structure, or characteristic described in conjunction with the embodiments may be included in at least one embodiment of the present application. The appearance of the phrase in various locations in the specification does not necessarily refer to the same embodiment, nor is it an independent or alternative embodiment that is mutually exclusive with other embodiments. It is explicitly and implicitly understood by those skilled in the art that the embodiments described herein may be combined with other embodiments.

[0032] In particular, the utility model provides a multi-concave cell culture sheet 100, which is suitable for culturing three-dimensional cell spheres, thereby achieving cell sphere culture with simple pretreatment, low economic cost and easy operation.

[0033] Figure 1 is a schematic structural diagram of a multi-concave cell culture sheet according to an embodiment of the utility model, Figure 2 is along Figure 1 A schematic cross-sectional view taken along the cutting line AA in FIG. Figure 3 A schematic structural diagram of a clamping portion according to an embodiment of the utility model. In this embodiment, Figure 1 and Figure 2 As shown, the utility model provides a multi-concave cell culture sheet 100, including a culture sheet body 10 and at least one clamping portion 20, wherein a plurality of downwardly recessed concave surfaces 11 are arranged on the top surface of the culture sheet body 10, and the concave surfaces 11 are used to culture cell balls, and the clamping portion 20 is an inverted convex structure, and each clamping portion 20 is arranged to protrude upward from the top surface of the culture sheet body 10, and the clamping portion 20 includes a connected connecting portion 21 and a limiting portion 22, and the two ends of the connecting portion 21 are used to connect the culture sheet body 10 and the limiting portion 22, and the limiting portion 22 protrudes from the side surface of the connecting portion 21, so that the limiting portion 22 forms a limiting surface 221 facing downward, so as to limit the tweezers clamped by the connecting portion 21, so as to prevent the multi-concave cell culture sheet 100 from falling due to insufficient friction on the surface of the connecting portion 21 when being clamped by the tweezers, thereby avoiding the loss of experimental materials.

[0034] In this embodiment, the connection part 21 and the limiting part 22 are detachably connected, a positioning column is provided at the top of the connection part 21, and a positioning hole is provided at the bottom of the limiting part 22. The positioning column and the positioning hole cooperate to fix the limiting part 22 and the connection part 21.

[0035] Figure 4 is a schematic structural diagram of a clamping portion according to another embodiment of the utility model. Figure 5 It is a schematic structural diagram of a clamping portion according to another embodiment of the utility model.

[0036] like Figure 4 and Figure 5As shown, in another embodiment, a groove 211 is provided on the peripheral side of one end of the connection part 21 close to the limiting part 22. Specifically, the groove 211 is provided on the connection part 21, and the tweezers clamp the position of the groove 211 of the connection part 21 when clamping the multi-concave cell culture sheet 100, and abut against the limiting surface 221 of the limiting part 22 when clamping the connection part 21. The groove 211 and the limiting part 22 both play a limiting role for the tweezers, so as to prevent the multi-concave cell culture sheet 100 from shaking when the tweezers clamp the multi-concave cell culture sheet 100, causing the multi-concave cell culture sheet 100 to slip. Here, the depth of the groove 211 is any value in the range of 0.2-0.6mm, that is, the depth of the groove 211 can be 0.2mm, 0.3mm, 0.4mm, 0.5mm or 0.6mm, or any other value in the range of 0.2-0.6mm, which is not specifically limited here and depends on the actual situation.

[0037] like Figure 4 As shown, in a preferred embodiment, the surface of the connecting portion 21 is provided with an anti-skid rubber sleeve 200. Specifically, the anti-skid rubber sleeve 200 is sleeved on the surface of one end of the connecting portion 21 close to the limiting portion 22 to improve the anti-skid performance of the connecting portion 21 and prevent the multi-concave cell culture sheet 100 from slipping due to insufficient friction of the connecting portion 21 when the tweezers clamp the connecting portion 21.

[0038] like Figure 5 As shown, in a preferred embodiment, the surface of the groove 211 is provided with an anti-slip feature. Specifically, the surface of the groove 211 of the connecting portion 21 is provided with an anti-slip feature, which further increases the friction force on the surface of the connecting portion 21, thereby improving the anti-slip performance of the connecting portion 21. Here, the anti-slip feature can be an anti-slip thread, an anti-slip pattern, an anti-slip coating or other anti-slip features that can play an anti-slip role, which are not specifically limited here and are determined according to actual conditions.

[0039] In a preferred embodiment, the connection portion 21 and the limiting portion 22 are movably connected, the connection method is threaded connection, and the thread of the connection portion 21 is set to have an anti-slip feature. Specifically, the connection portion 21 is provided with a thread, and the limiting portion 22 is provided with a threaded hole that matches the thread of the connection portion 21, so that the connection portion 21 and the limiting portion 22 are movably connected through the thread. In addition, the height of the thread of the connection portion 21 is greater than the height of the threaded hole of the limiting portion 22, so that after the connection portion 21 and the limiting portion 22 are fixedly connected, the redundant thread of the connection portion 21 serves as an anti-slip feature to increase the friction of the connection portion 21, thereby preventing the multi-concave cell culture sheet 100 from slipping due to insufficient friction of the connection portion 21 when the tweezers clamp the connection portion 21.

[0040] In this embodiment, the limiting portion 22 is cylindrical, and the connecting portion 21 is cylindrical. Specifically, the diameter of the limiting portion 22 is larger than the diameter of the connecting portion 21, so as to prevent the connecting portion 21 from sliding when the connecting portion 21 is clamped, so that the multi-concave cell culture sheet 100 can maintain balance in the horizontal direction, so as to avoid the outflow of substances on the surface of the culture sheet body 10, thereby avoiding unnecessary losses, and facilitating the collection of the obtained cell spheres into the required container. The diameter of the limiting portion 22 is any value in the range of 1.0-2.5 mm, and the height of the limiting portion 22 is any value in the range of 0.4-0.6 mm, that is, the diameter of the limiting portion 22 can be 1.0 mm, 1.5 mm, 2.0 mm or 2.5 mm, or any other value in the range of 1.0-2.5 mm, and the height of the connecting portion 21 can be 0.4 mm, 0.45 mm, 0.5 mm, 0.55 mm or 0.6 mm, or any other value in the range of 0.4-0.6 mm. The diameter of the connecting portion 21 is any value in the range of 0.5-2.0 mm, and the height of the connecting portion 21 is any value in the range of 1.0-3.0 mm, that is, the diameter of the connecting portion 21 can be 0.5 mm, 1.0 mm, 1.5 mm or 2.0 mm, or any other value in the range of 0.5-2.0 mm, and the height of the connecting portion 21 can be 1.0 mm, 2.0 mm or 3.0 mm, or any other value in the range of 1.0-3.0 mm.

[0041] In other embodiments, the limiting portion 22 may be a cuboid or a cube, and the connecting portion 21 may be a cuboid or a cube.

[0042] In this embodiment, there is one clamping portion 20, and the clamping portion 20 is located at the center of the culture sheet body 10. Specifically, the clamping portion 20 is disposed at the center of the culture sheet body 10, so that when the clamping portion 20 of the multi-concave cell culture sheet 100 is clamped, the culture sheet body 10 is kept balanced in the horizontal direction, and unnecessary losses caused by the outflow of fluid on the culture sheet body 10 are avoided.

[0043] In this embodiment, the multi-concave cell culture sheet 100 further includes a convex ring 40, which protrudes upward from the edge of the top surface of the culture sheet body 10, so that the convex ring 40 and the surface of the culture sheet body 10 are surrounded to form a receiving space. Specifically, the convex ring 40 is arranged along the periphery of the culture sheet body 10, and is surrounded to form a receiving space with the surface of the culture sheet body 10 to assist the cell spheres to fall into the central area of ​​the culture sheet body 10, to prevent the cell spheres from sliding off the multi-concave cell culture sheet 100, resulting in some cells not participating in the sphere formation, thereby improving the culture effect of the multi-concave cell culture sheet 100.

[0044] In a preferred embodiment, the height of the protruding ring 40 is 0.5 mm.

[0045] In this embodiment, the concave surface 11 has a preset height and its bottom is configured as a sphere. Specifically, the preset height of the concave surface 11 is any value in the range of 0-2.0 mm. The height of the concave surface 11 can be 0 mm, 0.5 mm, 1.0 mm, 1.5 mm or 2.0 mm, or any other value in the range of 0-2.0 mm, that is, the concave surface 11 may not be set with a preset height. The bottom of the concave surface 11 is configured as a sphere to form a cell sphere with the best culture effect. In other embodiments, the bottom of the concave surface 11 can be arc-shaped, rectangular, conical, or other shapes.

[0046] In this embodiment, the multi-concave cell culture sheet 100 is an integrated injection-molded structure, which is easy to manufacture and has low production costs, and can expand the production scale of the multi-concave cell culture sheet 100, thereby changing the traditional cell culture method. In addition, the injection molding method can adjust the size of the cell culture sheet according to demand.

[0047] In this embodiment, the smoothness of the multi-concave cell culture sheet 100 is at the nanometer level, which greatly reduces the adsorption of cells on the surface of the clampable multi-concave cell culture sheet 100, allowing the cells to slide smoothly toward the concave surface 11, thereby improving the efficiency and quality of cell sphering.

[0048] The technical features of the above-described embodiments may be arbitrarily combined. To make the description concise, not all possible combinations of the technical features in the above-described embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0049] The above-mentioned embodiments only express several implementation methods of the utility model, and the description is relatively specific and detailed, but it cannot be understood as limiting the scope of the utility model patent. It should be pointed out that for ordinary technicians in this field, several modifications and improvements can be made without departing from the concept of the utility model, which all belong to the protection scope of the utility model. Therefore, the protection scope of the utility model patent shall be based on the attached claims.

Claims

1. A multi-concave cell culture sheet, characterized in that: include: The culture sheet body has a plurality of concave surfaces sunken downwards on its top surface, and the concave surfaces are used for culturing cell spheres; At least one clamping portion, the clamping portion is an inverted convex structure, each of the clamping portions is configured to protrude upward from the top surface of the culture sheet body, the clamping portion includes a connected connecting portion and a limiting portion, the two ends of the connecting portion are used to connect the culture sheet body and the limiting portion, the limiting portion protrudes from the side of the connecting portion so that the limiting portion forms a limiting surface facing downward.

2. The multi-concave cell culture sheet according to claim 1, characterized in that: A groove is provided on the peripheral side of one end of the connecting portion close to the limiting portion.

3. The multi-concave cell culture sheet according to claim 2, characterized in that: The surface of the connecting part is provided with an anti-slip rubber sleeve.

4. The multi-concave cell culture sheet according to claim 2, characterized in that: The groove surface is provided with anti-slip features.

5. The multi-concave cell culture sheet according to claim 4, characterized in that: An external thread is provided on the outer peripheral surface of the upper part of the connecting part, and the limiting part is provided with a threaded hole that forms a threaded connection with the external thread. After the limiting part is connected to the connecting part, at least part of the external thread is exposed at the bottom of the limiting part, and the external thread exposed at the bottom of the limiting part is used to form the anti-slip feature.

6. The multi-concave cell culture sheet according to claim 5, characterized in that: The limiting portion is cylindrical, and the connecting portion is cylindrical.

7. The multi-concave cell culture sheet according to any one of claims 1 to 6, characterized in that: The number of the clamping part is one, and the clamping part is located at the center of the culture sheet body.

8. The multi-concave cell culture sheet according to claim 7, characterized in that: Also includes: The convex ring protrudes upward from the edge of the top surface of the culture sheet body, so that the convex ring and the surface of the culture sheet body are surrounded to form a receiving space.

9. The multi-concave cell culture sheet according to claim 8, characterized in that: The concave surface has a preset height and a bottom thereof is configured in a spherical shape.

10. The multi-concave cell culture sheet according to claim 1, characterized in that: The multi-concave culture sheet is an integral injection-molded structure.

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