Embedding box for full-length Swiss roll of intestinal tract of rodent

By designing and configuring the embedding box for configuring limits and cutting units, the problems of intestinal tissue morphological stability and structural integrity in traditional methods are solved, high-quality intestinal slices are achieved, and the operation process is simplified and research efficiency is improved.

CN222144666UActive Publication Date: 2024-12-10JINAN UNIVERSITY
View PDF 0 Cites 0 Cited by

Patent Information

Application Number
CN202422668652.4
Authority / Receiving Office
CN · China
Patent Type
Utility models(China)
Current Assignee / Owner
Filing Date
2024-11-04
Publication Date
2024-12-10
Estimated Expiration
2034-11-04

AI Technical Summary

Technical Problem

Traditional mouse intestinal pathological sectioning methods are difficult to maintain the morphological stability and structural integrity of intestinal tissues, and cannot effectively fix intestinal tissues of different sizes, resulting in poor section quality.

Method used

An embedded box is designed, including the box body and the box cover. The limiting unit is arranged on the box cover. The size of the limiting space is adjusted by rotating the plate and the slide chute, and the full-length Swiss roll of the intestine is fixed, reducing gaps and voids. A cutting unit is provided on the lid to cut the full length of the intestinal coils laterally.

Benefits of technology

Effectively fix intestinal tissue, prevent dispersion and damage, improve slice quality and observation effect, simplify operation, reduce errors, and improve research efficiency and continuous integrity of slices.

✦ Generated by Eureka AI based on patent content.

Smart Images

  • Figure CN222144666U_ABST
    Figure CN222144666U_ABST
Patent Text Reader

Abstract

The utility model relates to an embedding box for a full-length Swiss roll of a rodent intestinal tract. The embedding box comprises a box body with a hollow bottom, the box cover is rotatably connected with the box body; a limiting unit is arranged on the inner side face of the box cover and comprises a rotating plate and a bottom plate, the bottom plate is fixed to the box cover and circumferentially provided with a plurality of sliding grooves at certain intervals, sliding rods are arranged in the sliding grooves in a sliding mode, a limiting plate is arranged at one end of each sliding rod, and a connecting rod is perpendicularly arranged at the other end of each sliding rod. A limiting space used for containing the full-length Swiss roll of the intestinal tract is defined by the multiple limiting plates, a center column is arranged on the rotating plate, a plurality of arc-shaped grooves are formed in the rotating plate at certain intervals in the circumferential direction, and the connecting rods are slidably arranged in the arc-shaped grooves in a sleeved mode. The center column is rotated to enable the connecting rods to slide along the arc-shaped track of the arc-shaped groove, and the multiple sliding rods and the multiple limiting plates are driven to synchronously stretch out or retract in the radial direction, so that the size in the limiting space is adjusted.
Need to check novelty before this filing date? Find Prior Art

Description

Technical Field

[0001] The utility model relates to the technical field of embedding boxes, in particular to an embedding box for full-length Swiss rolls of rodent intestines. Background Art

[0002] In the field of biological and medical research, in-depth research on the intestinal tissue of rodents (such as mice) is an important means to understand physiological and pathological processes such as intestinal diseases, intestinal microbial communities, intestinal development and function. Among them, the pathological section analysis of the full-length mouse intestine has attracted much attention because it can fully display the structural characteristics of the intestine. However, the traditional method of making mouse intestinal pathological sections has many problems and shortcomings, especially in maintaining the integrity and morphological stability of the entire intestine. At present, the preparation of mouse intestinal pathological sections usually adopts the "Swiss roll" technology, that is, rolling the intestinal tissue into a cylindrical shape for easy slicing. However, this method often encounters the following problems in actual operation: 1) Difficulty in maintaining morphology: During dehydration, embedding and other treatments, the intestinal tissue is easy to spread out, making it difficult to maintain the overall morphology, which is not conducive to subsequent histological analysis; 2) Damage to structural integrity: Traditional embedding methods often cannot effectively fix intestinal tissue, especially when it needs to be fixed with a fine needle when curling to the end, which can easily cause mechanical damage to the intestinal tissue and affect the quality of the slices; 3) Different sizes of animal intestines: The sizes of the intestinal Swiss rolls of mice and rats are different, and the current instruments cannot achieve size limit adjustment; 4) Poor slice quality: Since the intestinal tissue is prone to form gaps, holes or deviations during the embedding process, it is easy to break and irregularly shaped when slicing, resulting in tissue waste, broken or incomplete intestinal rolls, and affecting the observation effect. Therefore, how to develop an embedding box that can limit and fix full-length Swiss rolls of animal intestines of different sizes and improve the quality of slices is an urgent problem that needs to be solved. Utility Model Content

[0003] In view of the problems existing in the prior art, the purpose of the utility model is to provide an embedding box for full-length Swiss rolls of rodent intestines, comprising a box body and a box cover, wherein a limiting unit is arranged on the box cover, and the limiting size is adjusted according to the size of the full-length Swiss roll of the intestine through the limiting unit, so that the intestinal tissue can be effectively fixed during the embedding process to prevent it from spreading and being damaged, while reducing the gaps and holes in the embedding block, ensuring the stability of the intestinal roll embedding, improving the section quality and observation effect, and facilitating the systematic evaluation of the research.

[0004] In order to achieve the above purpose, the utility model adopts the following technical solutions:

[0005] A packaging box for full-length Swiss rolls of rodent intestines, comprising: a box body with a hollow bottom; a box cover rotatably connected to the box body, wherein the box body and the box cover are configured to form an embedding space for accommodating full-length Swiss rolls of intestines inside them; wherein the inner side surface of the box cover is configured with a limiting unit for fixing the full-length Swiss rolls of intestines, wherein the limiting unit comprises a rotating plate and a bottom plate arranged in series up and down, wherein the bottom plate is fixed to the box cover and is circumferentially provided with a plurality of slide grooves at certain intervals, wherein a slide rod is slidably configured in the slide groove One end of the sliding rod has a limiting plate, and the other end of the sliding rod is vertically provided with a connecting rod, and the plurality of limiting plates define a limiting space for accommodating a full-length Swiss roll of the intestine, and the rotating plate is provided with a central column and a plurality of arc grooves are circumferentially provided at a certain interval, wherein the connecting rod can be slidably mounted in the arc groove, and the central column is rotated to make the connecting rod slide along the arc track of the arc groove, and drive the plurality of sliding rods and the plurality of limiting plates to synchronously extend or retract radially to adjust the size within the limiting space.

[0006] Furthermore, the box cover is also provided with a cutting unit for cutting the full-length Swiss roll of the intestine, and the cutting unit includes a rectangular plate, which surrounds the outside of the limiting unit and extends upward from the box cover to a certain height, wherein the rectangular plate has strip grooves on two opposite sides, and the two strip grooves are used to receive a cutting knife and allow the cutting knife to slide between the two strip grooves, so as to transversely cut the full-length Swiss roll of the intestine fixed on the limiting unit and form a cut surface, and when the box body and the box cover are in a closed state, the cut surface abuts against the bottom of the box body.

[0007] Furthermore, the box body has a first groove, the bottom of the first groove has a plurality of first communication ports, and the box cover has a plurality of second communication ports.

[0008] Furthermore, one end of the box body has a buckle groove, and the other end of the box cover has a fixing buckle that matches the buckle groove.

[0009] Furthermore, the rotating plate is also covered with a cover plate, the bottom of the cover plate has a second groove, the rotating plate is embedded in the second groove, and the cover plate has an interface for accommodating the central column.

[0010] Furthermore, the bottom plate has a plurality of first through holes, and the cover plate has a plurality of second through holes matching with the first through holes.

[0011] Furthermore, the arc trajectories of the plurality of arc grooves are the same.

[0012] Furthermore, the number of the limiting plates is six, and the limiting space surrounded by the six limiting plates forms a circular ring structure.

[0013] Furthermore, the inner diameter of the limiting space is greater than or equal to the diameter of the bottom plate.

[0014] Furthermore, the outer side surface of the box cover has a support rod, and the support rod is configured to support the box cover to maintain a horizontal position when the box body and the box cover are in an open state.

[0015] The utility model has the following advantages:

[0016] 1. The utility model is an embedding box for full-length Swiss rolls of the intestinal tract of rodents, comprising a box body and a box cover, and a limiting unit is arranged on the box cover. The limiting unit can effectively fix the intestinal tissue during the embedding process to prevent it from spreading and being damaged, and at the same time reduce the gaps and cavities in the embedding block, ensure the stability of the intestinal roll embedding, improve the quality of the slices and the observation effect, so as to facilitate the systematic evaluation of the research. The limiting unit adjusts the limiting size according to the size of the full-length Swiss roll of the intestinal tract, and is suitable for embedding the intestinal tissue of a variety of rodents, whether it is mice, rats or other small experimental animals.

[0017] 2. The embedding box of the utility model is easy to operate, has a high experimental success rate, and has stable tissue production. It can significantly ensure the production stability and uniformity of the intestinal Swiss roll, making the intestinal roll shape compact and the tissue morphology complete. The box cover is also equipped with a cutting unit. When the full-length intestinal Swiss roll is fixed in the limiting unit, the full-length intestinal Swiss roll is cut horizontally by a cutter to ensure that the position of the Swiss roll is not easy to move when cutting, and the cut surface tissue is continuous and complete, thereby improving the quality of the slice.

[0018] 3. The embedding box of the utility model can improve the research efficiency. The optimized embedding box design reduces the operation steps and time cost in the embedding process, reduces the error problem of uneven size when making intestinal rolls, and thus improves the efficiency of the entire research process. At the same time, high-quality sections also reduce errors and uncertainties in subsequent analysis. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 The utility model is a three-dimensional structural schematic diagram of an embedding box for a full-length Swiss roll of the intestinal tract of a rodent.

[0020] Figure 2 It is a three-dimensional structural schematic diagram from another angle of the embedding box for the full-length Swiss roll of rodent intestine of the utility model.

[0021] Figure 3 It is a three-dimensional structural schematic diagram of a limiting unit with a cover plate of the utility model.

[0022] Figure 4 It is a top view of the limiting unit without a cover plate of the utility model.

[0023] Figure 5 It is a three-dimensional exploded view of the limiting unit of the utility model.

[0024] Among them, 1 is a box body, 101 is a first groove, 101a is a first connecting port, 102 is a buckle groove, 2 is a box cover, 201 is a limiting unit, 201a is a rotating plate, 201a1 is a central column, 201a2 is an arc groove, 201b is a bottom plate, 201b1 is a sliding groove, 201b2 is a first through hole, 201c is a sliding rod, 201c1 is a limiting plate, 201c2 is a connecting rod, 201d is a cover plate, 201d1 is an interface, 201d2 is a second through hole, 202 is a cutting unit, 202a is a rectangular plate, 202b is a strip groove, 203 is a second connecting port, and 204 is a fixing buckle. DETAILED DESCRIPTION

[0025] The following description is essentially only exemplary and is not intended to limit the utility model, its application or use. It will be further understood that the term "include" and / or "comprising" specifies the existence of the features, wholes, steps, operations, elements and / or parts mentioned when used in this specification, but does not exclude the existence of one or more other features, wholes, steps, operations, elements, parts and / or its groups or add one or more other features, wholes, steps, operations, elements, parts and / or its groups. As used in this article, the term "and / or" includes any and all combinations of one or more of the associated listed items. It will be understood that when an element, component and / or part is referred to as "connected to another element, component and / or part", it can be directly connected to another element, component and / or part, or there can be an intermediate element. It will be understood that although the terms "first", "second" and the like can be used to describe various elements, components and / or parts in this article, these elements, components and / or parts should not be limited by these terms. These terms are only used to distinguish an element, component or part from another element, component or part. Therefore, the first element, component or part discussed below can be referred to as the second element, component or part without departing from the teaching of the utility model. Unless otherwise defined, all terms (including technical terms and scientific terms) used in this article have the same meanings as those commonly understood by those of ordinary skill in the field to which the utility model belongs. It will be further understood that terms such as those defined in commonly used dictionaries should be interpreted as having meanings consistent with their meanings in the relevant field and / or the context of this specification, and will not be interpreted in an idealized or overly formal sense, unless explicitly defined as such in this article.

[0026] It should be understood that, in order to clearly show the contents therein, the drawings herein are not drawn to scale, and the same or similar reference numerals indicate the same or similar components or parts. In addition, it should be understood that any embodiments described in this application and the technical features included therein can be combined with each other.

[0027] The present invention will be further described in detail below in conjunction with the accompanying drawings and specific implementation methods.

[0028] like Figure 1-5As shown, an embedding box for full-length Swiss rolls of rodent intestines comprises: a box body 1 with a hollow bottom; a box cover 2 rotatably connected to the box body 1, the box body 1 and the box cover 2 are configured to form an embedding space for accommodating full-length Swiss rolls of intestines inside them; wherein the inner side surface of the box cover 2 is configured with a limiting unit 201 for fixing the full-length Swiss rolls of intestines, the limiting unit 201 comprises a rotating plate 201a and a bottom plate 201b arranged in series up and down, the bottom plate 201b is fixed on the box cover 2 and is circumferentially provided with a plurality of slide grooves 201b1 at a certain interval, a slide rod 201c is slidably configured in the slide groove 201b1, and one end of the slide rod 201c has A limiting plate 201c1 and a connecting rod 201c2 are vertically arranged at the other end of the sliding rod 201c, and multiple limiting plates 201c1 define a limiting space for accommodating the full-length Swiss roll of the intestine. A central column 201a1 is arranged on the rotating plate 201a, and multiple arc grooves 201a2 are circumferentially arranged at certain intervals, wherein the connecting rod 201c2 can be slidably mounted in the arc groove 201a2, and the central column 201a1 is rotated to make the connecting rod 201c2 slide along the arc trajectory of the arc groove 201a2, and drive multiple sliding rods 201c and multiple limiting plates 201c1 to synchronously extend or retract radially to adjust the size in the limiting space. The center column 201a1 serves as the rotation axis, and the arc groove 201a2 thereon guides the sliding of the connecting rod 201c2 to achieve dynamic adjustment of the limiting space. The size of the limiting space can be accurately controlled by rotating the center column 201a1 according to the actual size of the intestinal sample, ensuring that the full-length Swiss roll of the intestine obtains the best fixation effect during the embedding process. The limiting plate 201c1 radially extends or retracts as the sliding rod 201c moves in the sliding groove 201b1, thereby achieving a tight fit and firm fixation of the intestinal sample. When the box body 1 and the box cover 2 are in the open state, the full-length Swiss roll of the intestine is wound on the center column 201a1 of the limiting unit 201. By rotating the center column 201a1, the connecting rod 201c2 slides along the arc groove 201a2, driving the sliding rod 201c and the limiting plate 201c1 to be synchronously adjusted to a suitable position, ensuring that the limiting space is tightly fitted to the intestinal sample. When the box body 1 and the box cover 2 are closed, the full-length Swiss roll of the intestine abuts against the bottom of the box body 1 to achieve position fixing. The position limiting unit 201 ensures that the intestinal roll is not easily displaced or has gaps during the embedding process, so that the intestinal roll is tight, thereby ensuring the integrity of the wax block molding, which is beneficial to the quality of subsequent sectioning and staining, and can effectively avoid damage to the intestinal tissue during the embedding and sectioning process, and maintain the integrity and morphology of the intestinal tissue.

[0029] like Figure 1 and Figure 2As shown, the box body 1 has a first groove 101, the bottom of the first groove 101 has a plurality of first communication ports 101a, and the box cover 2 has a plurality of second communication ports 203, and the first communication ports 101a and the second communication ports 203 are configured to allow the embedding solution to fill the embedding space to soak the intestinal full-length Swiss roll. One end of the box body 1 has a buckle groove 102, and the other end of the box cover 2 has a fixing buckle 204 that matches the buckle groove 102 to prevent the box cover 2 from loosening or opening due to solution pressure or external vibration during the embedding process.

[0030] like Figure 1 and Figure 2 As shown, the box cover 2 is also provided with a cutting unit 202 for cutting the full-length Swiss roll of the intestine, and the cutting unit 202 includes a rectangular plate 202a, which surrounds the outside of the limiting unit 201 and extends upward from the box cover 2 to a certain height, wherein the rectangular plate 202a has strip grooves 202b on two opposite sides, and the two strip grooves 202b are used to receive a cutter and allow the cutter to slide between the two strip grooves 202b, so as to transversely cut the full-length Swiss roll of the intestine fixed in the limiting unit 201 and form a cut surface, and when the box body 1 and the box cover 2 are in the closed state, the cut surface abuts against the bottom of the box body 1. The cutter is inserted from one of the strip grooves 202b and passes through the other strip groove 202b, and can slide freely between the two strip grooves 202b, thereby realizing transverse cutting of the full-length Swiss roll of the intestine fixed in the limiting unit 201, and forming a clear, flat, continuous and complete cut surface. The size of the rectangular plate 202a matches the size of the first groove 101, so that the box body 1 and the box cover 2 can be smoothly covered together.

[0031] like Figure 3-5 As shown, the rotating plate 201a is also covered with a cover plate 201d, and the bottom of the cover plate 201d has a second groove, and the rotating plate 201a is embedded in the second groove. The cover plate 201d has an interface 201d1 for accommodating the central column 201a1. Among them, the upper surface of the cover plate 201d serves as a bearing platform for the full-length Swiss roll of the intestine, effectively preventing possible damage during the rotation process, and the lower surface of the cover plate 201d is tightly fitted to the bottom plate 201b, and the diameter of the cover plate 201d is equal to the diameter of the bottom plate 201b. Among them, the cover plate 201d, the rotating plate 201a and the bottom plate 201b are all circular and coaxially arranged in series.

[0032] like Figure 3-5 As shown, the bottom plate 201b has a plurality of first through holes 201b2, and the cover plate 201d has a plurality of second through holes 201d2 that match the first through holes 201b2. The first through holes 201b2 and the second through holes 201d2 are configured to allow the embedding solution to pass through the limiting unit 201, so that the embedding solution can flow smoothly in the limiting unit 201.

[0033] like Figure 3-5 As shown, the arc trajectories of the plurality of arc grooves 201a2 are the same, the arc grooves 201a2 and the slide grooves 201b1 are arranged correspondingly up and down, and partially overlap, and the overlapping portion is used to receive the connecting rod 201c2, thereby realizing the series connection and linkage between the arc grooves 201a2 and the slide grooves 201b1.

[0034] like Figure 3-5 As shown, the limiting plate 201c1 is arc-shaped, and the number of the limiting plates 201c1 is six, and the limiting space surrounded by the six limiting plates 201c1 forms a circular ring structure. Among them, the corresponding numbers of the sliding rod 201c, the sliding groove 201b1, the connecting rod 201c2 and the arc groove 201a2 are all six. By rotating the central column 201a1, the six connecting rods 201c2 are synchronously slid along the same arc track in the six arc grooves 201a2, and at the same time, the six sliding rods 201c are driven to push the six limiting plates 201c1 to extend or retract radially, so as to achieve the size adjustment of the limiting space. Of course, the number of the limiting plates 201c1 can be adjusted according to actual needs, such as four, eight, ten, etc. Among them, the material of each component in the limiting unit 201 can be plastic.

[0035] like Figure 3-5 As shown, the inner diameter of the limiting space may be greater than or equal to the diameter of the bottom plate 201b. In this embodiment, when the limiting plate 201c1 is retracted, the inner diameter of the limiting space is equal to the diameter of the bottom plate 201b.

[0036] In an embodiment not shown, the center column 201a1 includes a sleeve and an inner tube slidably connected to the sleeve. The sleeve is fixed at the middle of the rotating plate 201a. The length of the center column 201a1 can be adjusted by sliding the inner tube to adapt to full-length Swiss rolls of intestines of different thicknesses.

[0037] In an embodiment not shown, the outer side of the box cover 2 has a support rod, which is configured to support the box cover 2 to maintain a horizontal position when the box body 1 and the box cover 2 are in an open state, thereby improving the quality of the full-length Swiss roll of the intestine.

[0038] The preparation method of the utility model is as follows:

[0039] Preparation before embedding: Quickly remove the full-length intestine from the rodent (mouse), and use forceps to remove the surrounding fat tissue and mesentery tissue from the intestinal tissue. Pay attention to maintaining the integrity and cleanliness of the intestine; use a gavage needle and a syringe filled with cold PBS to gently flush from the proximal end to the distal end to remove the contents and blood in the intestine and reduce contamination and interference; clamp the intestinal tube with fine-tipped forceps and cut it longitudinally from the distal end to the proximal end with fine-tipped scissors. Keep the tube side up, clamp the distal end with fine-tipped forceps, and rinse the inside of the intestinal tube in clean cold PBS.

[0040] Coiling and limiting of the full-length intestinal Swiss roll: Open the lid 2 of the embedding box, fit the distal end of the intestinal tissue to the central column 201a1, and slowly wrap the proximal intestinal tissue around the intestine to form a cylindrical full-length intestinal Swiss roll; at the same time, rotate the central column 201a1 to drive multiple limiting plates 201c1 to extend or retract to limit and fix the full-length intestinal Swiss roll. Then, soak the intestinal Swiss roll in 4% PFA, fix it at room temperature for 1 day, and then replace 4% PFA with 70% ethanol and soak it for 1 day.

[0041] Cutting of the full-length intestinal Swiss roll: insert the cutter between the two strip grooves 202b, and move the cutter to cut the full-length intestinal Swiss roll horizontally to form a Swiss roll with continuous and complete cut surface tissue, cover the box cover 2, make the cut surface end abut against the bottom of the box body 1 to fix the tissue position, and mark the tissue number on the outside of the box body 1 with a pencil.

[0042] Dehydration and immersion of full-length intestinal Swiss rolls: Tissue dehydration and wax immersion were performed, and the embedding boxes were immersed in 85% ethanol for 2 h, 95% ethanol for 2 h, anhydrous ethanol I for 30 min, anhydrous ethanol II for 30 min, anhydrous ethanol III for 30 min, a mixed solution of ethanol and xylene (ethanol: xylene = 1:1) for 30 min, xylene I for 30 min, xylene II for 30 min, and immersed in melted paraffin at 65°C overnight.

[0043] Finally, the full-length intestinal Swiss roll was taken out of the embedding box and placed at the bottom of the embedding mold. Wax water was poured to make a wax block, and paraffin sections could be performed after the wax block solidified and cooled.

[0044] In general, the utility model is used for the embedding box of the full-length Swiss roll of the intestinal tract of rodents, including a box body and a box cover, and a limiting unit is arranged on the box cover. The limiting unit can effectively fix the intestinal tissue during the embedding process to prevent it from spreading and being damaged, and at the same time reduce the gaps and cavities in the embedding block, ensure the stability of the embedding of the intestinal roll, improve the quality of the slices and the observation effect, so as to facilitate the systematic evaluation of the research. The limiting unit adjusts the limiting size according to the size of the full-length Swiss roll of the intestinal tract, and is suitable for the embedding of intestinal tissues of various rodents, whether mice, rats or other small experimental animals. The embedding box of the utility model is easy to operate, has a high experimental success rate, and is stable in tissue production. It can significantly ensure the production stability and uniformity of the intestinal Swiss roll, so that the intestinal roll shape is tight and the tissue morphology is complete. The box cover is also equipped with a cutting unit. When the full-length Swiss roll of the intestinal tract is fixed in the limiting unit, the full-length Swiss roll of the intestinal tract is cut horizontally by a cutter, so that the position of the Swiss roll is not easy to move when cutting, and the cut surface tissue is continuous and complete, thereby improving the slice quality. The embedding box of the utility model can improve the research efficiency. The optimized embedding box design reduces the operation steps and time cost in the embedding process, reduces the error problem of uneven size when making sausage rolls, and thus improves the efficiency of the entire research process. At the same time, high-quality sections also reduce errors and uncertainties in subsequent analysis.

[0045] The above embodiments are preferred implementation modes of the present invention, but the implementation modes of the present invention are not limited by the above embodiments. Any other changes, modifications, substitutions, combinations, and simplifications that do not deviate from the spirit and principle of the present invention should be equivalent replacement methods and are included in the protection scope of the present invention.

Claims

1. An embedding box for full-length Swiss rolls of rodent intestines, characterized in that: include: The box body is hollowed out at the bottom; a box cover rotatably connected to the box body, the box body and the box cover being configured to form an embedding space therein for accommodating the intestinal full-length Swiss roll; In which, the inner side surface of the box cover is provided with a limiting unit for fixing the full-length Swiss roll of the intestine, and the limiting unit includes a rotating plate and a bottom plate arranged in series up and down, the bottom plate is fixed on the box cover and has a plurality of sliding grooves circumferentially arranged at a certain interval, a sliding rod is slidingly arranged in the sliding groove, one end of the sliding rod has a limiting plate, and the other end of the sliding rod is vertically provided with a connecting rod, and a plurality of the limiting plates define a limiting space for accommodating the full-length Swiss roll of the intestine, a central column is provided on the rotating plate and a plurality of arc grooves are circumferentially arranged at a certain interval, wherein the connecting rod can be slidably mounted in the arc groove, and the central column is rotated to make the connecting rod slide along the arc trajectory of the arc groove, and drive the plurality of the sliding rods and the plurality of the limiting plates to synchronously extend or retract radially to adjust the size within the limiting space.

2. The embedding box for full-length Swiss roll of rodent intestine according to claim 1, characterized in that: The box cover is also provided with a cutting unit for cutting the full-length Swiss roll of the intestine, and the cutting unit includes a rectangular plate, which surrounds the outside of the limiting unit and extends upward from the box cover to a certain height, wherein the rectangular plate has strip grooves on two opposite sides, and the two strip grooves are used to receive a cutting knife and allow the cutting knife to slide between the two strip grooves, so as to transversely cut the full-length Swiss roll of the intestine fixed on the limiting unit and form a cut surface, and when the box body and the box cover are in a closed state, the cut surface abuts against the bottom of the box body.

3. The embedding box for full-length Swiss roll of rodent intestine according to claim 1, characterized in that: The box body is provided with a first groove, the bottom of the first groove is provided with a plurality of first communication ports, and the box cover is provided with a plurality of second communication ports.

4. The embedding box for full-length Swiss roll of rodent intestine according to claim 1, characterized in that: One end of the box body is provided with a buckle groove, and the other end of the box cover is provided with a fixing buckle matched with the buckle groove.

5. The embedding box for full-length Swiss roll of rodent intestine according to claim 1, characterized in that: The rotating plate is also covered with a cover plate, the bottom of the cover plate has a second groove, the rotating plate is embedded in the second groove, and the cover plate has an interface for accommodating the central column.

6. The embedding box for full-length Swiss roll of rodent intestine according to claim 5, characterized in that: The bottom plate has a plurality of first through holes, and the cover plate has a plurality of second through holes matched with the first through holes.

7. The embedding box for full-length Swiss roll of rodent intestine according to claim 1, characterized in that: The arc-shaped trajectories of the plurality of arc-shaped grooves are the same.

8. The embedding box for full-length Swiss roll of rodent intestine according to claim 1, characterized in that: The number of the limiting plates is six, and the limiting space surrounded by the six limiting plates forms a circular ring structure.

9. The embedding box for full-length Swiss roll of rodent intestine according to claim 8, characterized in that: The inner diameter of the limiting space is greater than or equal to the diameter of the bottom plate.

10. The embedding box for full-length Swiss roll of rodent intestine according to claim 1, characterized in that: The outer side surface of the box cover is provided with a support rod, and the support rod is configured to support the box cover to maintain a horizontal position when the box body and the box cover are in an open state.