Cell culture separation equipment

Abstract

The utility model provides cell culture separation equipment, which belongs to the technical field of cell separation, the cell culture separation equipment comprises a centrifugal tank, a suction pipe, a peristaltic pump and a motor, the centrifugal tank is provided with a centrifugal cavity and a connecting end, and the bottom wall of the centrifugal cavity is provided with a conical bulge extending towards the connecting end; one end of the suction pipe penetrates through the connecting end and extends into the centrifugal cavity, and the connecting end is rotatably connected with the suction pipe; the input end of the peristaltic pump is communicated with the other end of the suction pipe through a guide pipe; and the output end of the motor is connected with the centrifugal tank. And centrifugally separating the cells. The cell culture separation equipment pumps waste liquid, impurities and the like into the waste liquid bag for storage through the peristaltic pump, the suction pipe and the guide pipe. The probability that waste liquid, impurities and the like are mixed with finished cells is reduced, residues of the waste liquid, the impurities and the like can be reduced, and therefore the labor amount of operators can be reduced.

Description

Technical Field

[0001] The utility model belongs to the technical field of cell separation, and specifically relates to a cell culture separation device. Background Art

[0002] Cell culture technology requires the separation of cell culture fluid and impurities. The existing cell separation process mainly uses a traditional centrifuge to inject the cells to be separated into a centrifuge tube, and then put the centrifuge tube into the centrifuge for high-speed rotation to separate the cell culture fluid and impurities from the cells. Finally, the cell culture fluid and impurities in the centrifuge tube are manually removed.

[0003] However, in the process of manually removing cell culture fluid and impurities, since the centrifuge tube is in a static state, firstly, the cell culture fluid and impurities will be mixed with the finished cells, making it easy to remove the finished cells; secondly, when the cell culture fluid and impurities are removed to the final stage, a large amount of cell culture fluid and impurities will still remain, increasing the difficulty of removal and thus increasing the workload. Utility Model Content

[0004] In order to make up for the above shortcomings, the utility model provides a cell culture separation device, aiming to solve the problem that the existing centrifuge tube is inconvenient to remove cell culture fluid and impurities.

[0005] The utility model is specifically achieved in this way:

[0006] A cell culture separation device, comprising:

[0007] A centrifugal tank, the centrifugal tank having a centrifugal chamber and a connecting end, the bottom wall of the centrifugal chamber being provided with a conical protrusion extending toward the connecting end;

[0008] A suction tube, one end of which passes through the connecting end and extends into the centrifugal chamber, and the connecting end is rotatably connected to the suction tube;

[0009] a peristaltic pump, wherein an input end of the peristaltic pump is connected to the other end of the suction tube through a conduit;

[0010] A motor, wherein the output end of the motor is connected to the centrifugal tank.

[0011] Furthermore, the centrifugal tank comprises a tank cover and a tank body, the connecting end is formed on the tank cover, and the tank cover is detachably connected to the tank body.

[0012] Furthermore, the tank body includes a first shell and a second shell, the second shell is arranged in the first shell, and the output end of the motor is connected to the first shell.

[0013] Furthermore, a plurality of slide grooves extending in the axial direction are provided in the first shell, and the plurality of slide grooves are arranged at intervals around the axis of the first shell. The second shell is provided with a sliding protrusion corresponding to each of the slide grooves, and the sliding protrusion cooperates with the slide groove.

[0014] Furthermore, the tank cover is provided with a first annular groove extending radially at one end away from the connecting end, and the first shell is provided with a second annular groove extending radially at one end close to the tank cover, so that the tank cover is connected to the first shell in a socket connection.

[0015] Furthermore, an internal thread is provided in the first annular groove, and an external thread is provided in the second annular groove, so that the tank cover is threadably connected to the first shell body.

[0016] Furthermore, the tank cover is provided with a plurality of push rods, and the plurality of push rods are arranged at intervals around the axis of the tank cover.

[0017] Furthermore, a filter screen is provided at one end of the suction pipe close to the conical protrusion.

[0018] Further, it includes a connecting tube, which is rotatably arranged at the connecting end, and the suction tube is located in the connecting tube. The connecting tube is provided with at least one pair of stepped holes symmetrically along the axial direction, and an elastic member, a base and a ball are sequentially provided in each of the stepped holes facing the suction tube, and the connecting tube is provided with a plurality of pairs of limit grooves spaced apart along the axial direction, and the ball cooperates with the limit groove to limit the length of the suction tube extending into the centrifugal chamber.

[0019] Furthermore, it comprises a cylinder and a bracket, wherein the cylinder is arranged on the bracket, and the centrifugal tank, the suction tube and the peristaltic pump are arranged in the cylinder.

[0020] Compared with the prior art, the beneficial effects of the utility model are:

[0021] (1) During cell centrifugation, waste liquid and impurities are pumped into waste liquid bags for storage through peristaltic pumps, suction tubes and catheters. This can reduce the chance of waste liquid and impurities mixing with finished cells and reduce the residual waste liquid and impurities, thereby reducing the workload of operators.

[0022] (2) The second shell serves as an inner container, which facilitates the insertion or removal of the second shell, thereby improving the convenience of adding cells, pouring out finished cells, or cleaning operations.

[0023] (3) The length of the suction tube extending into the centrifugal chamber can be changed by adjusting the elastic member, the ball bearing and the limit groove to adapt to the height of the cell accumulation in the centrifugal chamber, thereby preventing the suction tube from being blocked during suction. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] In order to more clearly illustrate the technical solution of the implementation mode of the utility model, the drawings required for use in the implementation mode will be briefly introduced below. It should be understood that the following drawings only show certain embodiments of the utility model and therefore should not be regarded as limiting the scope. For ordinary technicians in this field, other relevant drawings can be obtained based on these drawings without paying creative work.

[0025] Figure 1 It is a stereoscopic diagram of a cell culture separation device provided by an embodiment of the utility model;

[0026] Figure 2 is a vertical cross-sectional view of a cell culture separation device provided by an embodiment of the utility model;

[0027] Figure 3 It is a structural schematic diagram of the first shell provided by an embodiment of the utility model;

[0028] Figure 4 It is a structural schematic diagram of the second housing provided by an embodiment of the utility model;

[0029] Figure 5 It is a schematic diagram of the structure of the suction tube provided by the embodiment of the utility model;

[0030] Figure 6 is a cross-sectional view of a can cover provided by an embodiment of the utility model;

[0031] Figure 7 yes Figure 6 A partial enlarged view of the middle part;

[0032] Figure 8 It is a structural schematic diagram of a cylinder and a bracket provided in an embodiment of the utility model;

[0033] Fig. 9 It is a vertical cross-sectional view of the cylinder and the bracket provided in the embodiment of the utility model.

[0034] Explanation of the reference numerals: 100, centrifugal tank; 101, centrifugal chamber; 102, connecting end; 103, conical protrusion; 110, tank cover; 111, first annular groove; 112, push rod; 120, tank body; 121, first shell; 1211, slide groove; 1212, second annular groove; 122, second shell; 1221, sliding protrusion; 200, suction pipe; 210, filter screen; 220, limiting groove; 300, motor; 400, connecting pipe; 401, stepped hole; 410, elastic member; 420, support; 430, ball bearing; 500, cylinder; 600, bracket. DETAILED DESCRIPTION

[0035] In order to make the purpose, technical solution and advantages of the implementation of the utility model clearer, the technical solution in the implementation of the utility model will be clearly and completely described below in conjunction with the drawings in the implementation of the utility model. Obviously, the described implementation is a part of the implementation of the utility model, not all of the implementations. Based on the implementation of the utility model, all other implementations obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0036] Therefore, the following detailed description of the embodiments of the present invention provided in the accompanying drawings is not intended to limit the scope of the claimed invention, but merely represents selected embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in the field without creative work are within the scope of protection of the present invention.

[0037] It should be noted that similar reference numerals and letters denote similar items in the following drawings, and therefore, once an item is defined in one drawing, further definition and explanation thereof is not required in subsequent drawings.

[0038] In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise" and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the referred device or element must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present invention.

[0039] In addition, the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include one or more of the features. In the description of the present utility model, the meaning of "plurality" is two or more, unless otherwise clearly and specifically defined. Example

[0040] See attached Figure 1~2As shown, the utility model provides a technical solution: a cell culture separation device, comprising a centrifugal tank 100, a suction tube 200, a peristaltic pump (not shown in the figure) and a motor 300, the centrifugal tank 100 has a centrifugal chamber 101 and a connecting end 102, the bottom wall of the centrifugal chamber 101 is provided with a conical protrusion 103 extending toward the connecting end 102; one end of the suction tube 200 passes through the connecting end 102 and extends into the centrifugal chamber 101, and the connecting end 102 is rotatably connected to the suction tube 200; the input end of the peristaltic pump is connected to the other end of the suction tube 200 through a conduit; the output end of the motor 300 is connected to the centrifugal tank 100.

[0041] For example, a feed pipe and a discharge pipe can be provided on the centrifugal tank 100, wherein the feed pipe is located on the arc surface of the centrifugal tank 100, and the discharge pipe is close to the conical protrusion 103. The suction pipe 200 can be fixed to the centrifugal tank 100. The output end of the peristaltic pump can be connected to the waste liquid bag through a conduit. The motor 300 can be a reduction motor 300, a stepping motor 300, or a servo motor 300, etc.

[0042] In actual application, the motor 300 drives the centrifugal tank 100 to perform centrifugal motion. Affected by the density, the waste liquid and impurities gradually float up, while the finished cells gradually fall on the conical protrusion 103, so that the cells and impurities are separated. After a certain period of centrifugal motion, the rotation speed of the centrifugal tank 100 is reduced, and the peristaltic pump is turned on. The waste liquid and impurities are pumped into the waste liquid bag for storage through the peristaltic pump, the suction tube 200 and the catheter. A large amount of waste liquid and impurities can be removed, and the remaining waste liquid and impurities can be simply removed by the operator, thereby reducing the workload of the operator.

[0043] See attached Figure 1~2 As shown, in an optional embodiment, the centrifuge tank 100 includes a tank cover 110 and a tank body 120, the connecting end 102 is formed on the tank cover 110, and the tank cover 110 and the tank body 120 are detachably connected.

[0044] For example, the tank cover 110 and the tank body 120 may be fixed by a lock or the like.

[0045] In practical applications, the centrifuge tank 100 is designed to be split, firstly, to facilitate adding cells or pouring out finished cells, and secondly, to facilitate cleaning of the centrifuge tank 100.

[0046] See attached Figures 2~4 As shown, in an optional embodiment, the tank body 120 includes a first shell 121 and a second shell 122 , the second shell 122 is disposed in the first shell 121 , and the output end of the motor 300 is connected to the first shell 121 .

[0047] For example, the tank cover 110 and the first shell 121 can be fixed by a lock or the like.

[0048] In practical applications, the tank body 120 is designed to be split, so that the second shell 122 acts as an inner container, which facilitates the placement or removal of the second shell 122, thereby improving the convenience of adding cells, pouring out finished cells or cleaning operations.

[0049] See attached Figure 3~4 As shown, in an optional embodiment, a plurality of slide grooves 1211 extending axially are provided in the first shell 121, and the plurality of slide grooves 1211 are arranged at intervals around the axis of the first shell 121, and a sliding protrusion 1221 is provided in the second shell 122 corresponding to each slide groove 1211, and the sliding protrusion 1221 cooperates with the slide groove 1211.

[0050] In practical applications, the cooperation between the sliding protrusion 1221 and the sliding groove 1211 generates a resistance effect on the second shell 122 , so that the second shell 122 can rotate together with the first shell 121 .

[0051] See attached Figure 2~3 As shown in Figure 6, in an optional embodiment, the end of the tank cover 110 away from the connecting end 102 is provided with a first annular groove 111 extending radially, and the end of the first shell 121 close to the tank cover 110 is provided with a second annular groove 1212 extending radially, so that the tank cover 110 and the first shell 121 are connected in a socket manner.

[0052] For example, the tank cover 110 and the first shell 121 can be fixed by a lock or the like.

[0053] In practical applications, such a design allows the connection between the tank cover 110 and the first shell 121 to be offset from each other, thereby preventing leakage of waste liquid.

[0054] See attached Figure 2~3 As shown in FIG. 6 , in an optional embodiment, an internal thread is provided in the first annular groove 111 , and an external thread is provided in the second annular groove 1212 , so that the tank cover 110 is threadedly connected to the first shell 121 .

[0055] For example, a sealing ring is provided between the tank cover 110 and the first shell 121, or a waterproof raw tape is provided between the internal thread and the external thread.

[0056] In practical applications, such a design can strengthen the sealing of the containing cavity and further prevent leakage of waste liquid.

[0057] See attached Figure 1~2 As shown, in an optional embodiment, a plurality of push rods 112 are provided on the tank cover 110 , and the plurality of push rods 112 are arranged at intervals around the axis of the tank cover 110 .

[0058] In practical applications, the push rod 112 can play an auxiliary role, making it easier for operators to rotate the tank cover 110 .

[0059] See attached Figure 2 and 5 As shown, in an optional embodiment, a filter screen cover 210 is provided at one end of the suction pipe 200 close to the conical protrusion 103 .

[0060] In practical applications, when waste liquid and impurities are pumped out, the filter screen 210 can play a filtering role to prevent the finished cells from being sucked into the suction tube 200 .

[0061] See attached Figure 2 and 6 As shown in Figure 7, in an optional embodiment, it includes a connecting tube 400, which is rotatably arranged at the connecting end 102, and the suction tube 200 is located in the connecting tube 400. The connecting tube 400 is provided with at least one pair of stepped holes 401 symmetrical along the axial direction, and each stepped hole 401 is provided with an elastic member 410, a base and a ball 430 in sequence facing the suction tube 200. The connecting tube 400 is provided with a plurality of pairs of limit grooves 220 arranged at intervals along the axial direction, and the ball 430 cooperates with the limit groove 220 to limit the length of the suction tube 200 extending into the centrifugal chamber 101.

[0062] For example, the elastic member 410 may be a spring or a rubber elastic pad.

[0063] In actual application, when the suction tube 200 is stationary, the elastic member 410 makes the ball 430 cooperate with the limiting groove 220 to limit the suction tube 200. When the suction tube 200 moves, as the length of the suction tube 200 in the centrifugal chamber 101 changes, the ball 430 pushes the base to squeeze the elastic member 410 to produce compression deformation, and the ball 430 passes through or is located between two limiting grooves 220, or the elastic member 410 extends to push the base and the ball 430 to move toward the limiting groove 220 and be located in the limiting groove 220. Affected by the suction force, the suction tube 200 should be away from the cells. Therefore, by changing the length of the suction tube 200 extending into the centrifugal chamber 101 to adapt to the height of the cell accumulation in the centrifugal chamber 101, it is prevented that the suction tube 200 is blocked during suction.

[0064] See attached Figure 2 and 6 As shown in Figure 7, in an optional embodiment, it includes a barrel 500 and a bracket 600, the barrel 500 is arranged on the bracket 600, and the centrifugal tank 100, the suction tube 200 and the peristaltic pump are arranged in the barrel 500.

[0065] In practical applications, the cylinder 500 can isolate the centrifuge tank 100 from the outside world to prevent leakage of waste liquid and the like from contaminating the test area.

[0066] The above are only preferred embodiments of the present invention and are not intended to limit the present invention. For those skilled in the art, the present invention may be modified and varied in various ways. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A cell culture separation device, characterized in that: include: A centrifugal tank (100), the centrifugal tank (100) comprising a centrifugal chamber (101) and a connecting end (102), the bottom wall of the centrifugal chamber (101) being provided with a conical protrusion (103) extending towards the connecting end (102); a suction tube (200), one end of the suction tube (200) passing through the connecting end (102) and extending into the centrifugal chamber (101), the connecting end (102) being rotatably connected to the suction tube (200); a peristaltic pump, the input end of the peristaltic pump being connected to the other end of the suction tube (200) via a conduit; A motor (300), wherein an output end of the motor (300) is connected to the centrifugal tank (100).

2. The cell culture separation device according to claim 1, characterized in that: The centrifugal tank (100) comprises a tank cover (110) and a tank body (120); the connection end (102) is formed on the tank cover (110); and the tank cover (110) and the tank body (120) are detachably connected.

3. The cell culture separation device according to claim 2, characterized in that: The tank (120) comprises a first shell (121) and a second shell (122); the second shell (122) is arranged in the first shell (121); and the output end of the motor (300) is connected to the first shell (121).

4. The cell culture separation device according to claim 3, characterized in that: A plurality of slide grooves (1211) extending along the axial direction are provided in the first shell (121), and the plurality of slide grooves (1211) are arranged at intervals around the axis of the first shell (121), and the second shell (122) is provided with a sliding protrusion (1221) corresponding to each of the slide grooves (1211), and the sliding protrusion (1221) cooperates with the slide groove (1211).

5. The cell culture separation device according to claim 4, characterized in that: A first annular groove (111) extending radially is provided at one end of the tank cover (110) away from the connecting end (102), and a second annular groove (1212) extending radially is provided at one end of the first shell (121) close to the tank cover (110), so that the tank cover (110) and the first shell (121) are connected in a socket-type manner.

6. The cell culture separation device according to claim 5, characterized in that: An internal thread is provided in the first annular groove (111), and an external thread is provided in the second annular groove (1212), so that the tank cover (110) is threadably connected to the first shell (121).

7. The cell culture separation device according to claim 2, characterized in that: The tank cover (110) is provided with a plurality of push rods (112), and the plurality of push rods (112) are arranged at intervals around the axis of the tank cover (110).

8. The cell culture and separation device according to any one of claims 1 to 7, characterized in that: A filter screen cover (210) is provided at one end of the suction pipe (200) close to the conical protrusion (103).

9. The cell culture and separation device according to any one of claims 1 to 7, characterized in that: The invention comprises a connecting tube (400), wherein the connecting tube (400) is rotatably arranged at the connecting end (102), the suction tube (200) is located in the connecting tube (400), the connecting tube (400) is provided with at least one pair of stepped holes (401) symmetrical along the axial direction, each of the stepped holes (401) is provided with an elastic member (410), a base and a ball (430) in sequence facing the suction tube (200), and the connecting tube (400) is provided with a plurality of pairs of limiting grooves (220) spaced apart along the axial direction, and the ball (430) cooperates with the limiting groove (220) to limit the length of the suction tube (200) extending into the centrifugal chamber (101).

10. The cell culture separation device according to claim 9, characterized in that: It comprises a barrel (500) and a support (600), wherein the barrel (500) is arranged on the support (600), and the centrifugal tank (100), the suction tube (200) and the peristaltic pump are arranged inside the barrel (500).

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