Anaerobic bacteria cultivation box
By using a combination of sealing blocks and L-shaped tubes in the anaerobic bacterial culture box, ensure that the nutrient solution is added under a sealed environment, avoid oxygen pollution, and achieve uniform dripping and sealing of the nutrient solution through an annular drip and spring reset mechanism. The problem of oxygen pollution is solved to ensure the normal growth and uniform growth environment of anaerobic bacteria.
Patent Information
- Application Number
- CN202420250302.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-02-01
- Publication Date
- 2024-12-13
- Estimated Expiration
- 2034-02-01
AI Technical Summary
During the cultivation of existing anaerobic bacteria, when the dropper is inserted into the sealed hole, oxygen will enter the incubation box, contaminating the anaerobic bacteria, affecting their normal growth, and limiting their promotion and application.
Anaerobic bacterial cultivation box was designed, using a combination of sealing blocks and L-shaped tubes. The nutrient solution gradually moved downwards through the sealing blocks and communicated with the L-shaped tubes. The spray plate dripped to the Petri dish to ensure the sealing environment and prevent oxygen from entering; At the same time, the dripper is distributed in an annular shape and drips evenly to avoid growth differences; use spring reset sealing to provide appropriate temperature.
It realizes the addition of nutrient solution in a sealed environment to avoid oxygen pollution, ensure the normal growth of anaerobic bacteria, and reduce growth differences and temperature discomfort through uniform dripping and temperature control.
Smart Images
Figure CN222160118U_ABST
Abstract
Description
Technical Field
[0001] The present application relates to the technical field of anaerobic bacteria cultivation, and in particular to an anaerobic bacteria cultivation box. Background Art
[0002] Anaerobic bacteria have broad application prospects and are widely used in the metallurgical industry, sewage treatment, disease detection and treatment. However, most anaerobic bacteria are uncultivable, which brings inconvenience to their development and utilization. Anaerobic bacteria grow better under anaerobic conditions than in aerobic environments, while bacteria that cannot grow on the surface of solid culture media in the air lack a complete metabolic enzyme system, and their energy metabolism is carried out in the form of anaerobic fermentation.
[0003] However, in actual applications, most existing anaerobic bacteria require regular addition of nutrient solution during the cultivation process to ensure the normal growth of mycelium. Usually, a dropper is used to absorb the nutrient solution and then inserted into the sealing hole on the cultivation box to complete the addition of the nutrient solution. However, at the moment the dropper is inserted into the sealing hole, a small amount of oxygen will still enter the cultivation box from the opened sealing hole, which will cause the anaerobic mycelium to be easily contaminated and affect the normal growth of anaerobic bacteria, which is not conducive to promotion and use.
[0004] Therefore, those skilled in the art provide an anaerobic bacteria cultivation box to solve the problems raised in the above background technology. Utility Model Content
[0005] In order to solve the problem raised in the above background technology that at the moment when the dropper is inserted into the sealing hole, a small amount of oxygen will still enter the cultivation box from the opened sealing hole, which will cause the anaerobic hyphae to be easily contaminated and affect the normal growth of anaerobic bacteria, which is not conducive to promotion, the present application provides an anaerobic bacteria cultivation box.
[0006] An anaerobic bacteria cultivation box provided in the present application adopts the following technical solution: it includes a base, a sealing cover is adapted to be placed on the top side of the base, a columnar groove is opened on the top side of the sealing cover, a sealing block is slidably connected to the inner wall of the columnar groove, the sealing block is fitted with the inner wall of the columnar groove, the left and right sides of the columnar groove are fixedly connected with L-shaped tubes, the top inner wall of the sealing cover is fixedly connected with a spray plate, the top side of the spray plate is fixedly connected with two L-shaped tubes, and a culture dish is placed on the top side of the base.
[0007] Preferably, a plurality of drippers are fixedly connected to the bottom side of the spray plate, and the plurality of drippers are equidistantly distributed in a ring shape.
[0008] Preferably, a spring is fixedly connected to the inner wall of the bottom side of the columnar groove, and the top end of the spring is fixedly connected to the bottom side of the sealing block.
[0009] Preferably, a sealing rubber ring is fixedly connected to the top side of the base, an annular sealing groove is formed on the bottom side of the sealing cover, and the sealing rubber ring is in contact with the inner wall of the annular sealing groove.
[0010] Preferably, a plurality of electric heating rods are fixedly mounted on the inner side of the sealing cover, and the plurality of electric heating rods are all electrically connected to an external power source.
[0011] In summary, this application includes the following beneficial technical effects:
[0012] 1. By setting the sealing block and the L-shaped tube, the culture dish needs to regularly add nutrient solution when cultivating anaerobic bacteria. When adding nutrient solution, the external nutrient solution injection tube is squeezed against the sealing block. As the sealing block gradually moves downward, the external nutrient solution injection tube will be connected with the two L-shaped tubes. The nutrient solution will flow into the inside of the spray plate along the L-shaped tube and drip into the culture dish. Thus, the nutrient solution can be added in a sealed environment to avoid oxygen entry and anaerobic mycelium contamination, thereby ensuring the normal growth of anaerobic bacteria.
[0013] 2. By arranging a plurality of drippers in a circular shape and equidistantly distributed, the nutrient solution can be evenly dripped into the culture dish, thereby avoiding differences in the growth degree of mycelium in different areas due to different amounts of nutrient solution added. BRIEF DESCRIPTION OF THE DRAWINGS
[0014] Figure 1 It is a left cross-sectional structural schematic diagram of an anaerobic bacteria cultivation box in an embodiment of the present application;
[0015] Figure 2 It is a right sectional structural schematic diagram of an anaerobic bacteria cultivation box in an embodiment of the present application;
[0016] Figure 3 This is an anaerobic bacteria cultivation box in the embodiment of the present application. Figure 2 Schematic diagram of the structure with part A enlarged.
[0017] Explanation of the reference numerals: 1. base; 2. sealing cover; 3. sealing block; 4. L-shaped tube; 5. spray plate; 6. culture dish; 7. dripper; 8. spring; 9. sealing rubber ring; 10. electric heating rod. DETAILED DESCRIPTION
[0018] The following will be combined with the attached Figure 1-3 The technical solution of the utility model is described clearly and completely. Obviously, the described embodiments are part of the embodiments of the utility model, not all of them. Based on the embodiments of the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.
[0019] In the description of the present utility model, it should be noted that the orientation or position relationship indicated by the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inside", "outside", etc. is based on the orientation or position relationship shown in the drawings, which is only for the convenience of describing the present utility model and simplifying the description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation of the present utility model. In addition, the terms "first", "second", and "third" are only used for descriptive purposes and cannot be understood as indicating or implying relative importance. In the description of the present utility model, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be directly connected, or indirectly connected through an intermediate medium, or it can be the internal connection of two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present utility model can be understood according to specific circumstances. In addition, the technical features involved in the different embodiments of the present utility model described below can be combined with each other as long as they do not conflict with each other.
[0020] The present application embodiment discloses an anaerobic bacteria cultivation box, referring to Figure 1-3 , including a base 1, a sealing cover 2 is adapted to be placed on the top side of the base 1, a columnar groove is opened on the top side of the sealing cover 2, a sealing block 3 is slidably connected to the inner wall of the columnar groove, the sealing block 3 fits the inner wall of the columnar groove, and the left and right sides of the columnar groove are fixedly connected with L-shaped tubes 4, the inner wall of the top side of the sealing cover 2 is fixedly connected with a spray plate 5, the top side of the spray plate 5 is fixedly connected with two L-shaped tubes 4, and a culture dish 6 is placed on the top side of the base 1; by setting the sealing block 3 and the L-shaped tube 4, the culture dish 6 needs to regularly add nutrient solution when cultivating anaerobic bacteria. When adding nutrient solution, the injection pipe of the external nutrient solution is squeezed against the sealing block 3. As the sealing block 3 gradually moves downward, the injection pipe of the external nutrient solution will be connected with the two L-shaped tubes 4 at this time, and the nutrient solution will enter the interior of the spray plate 5 along the L-shaped tube 4 and drip into the culture dish 6, so that the addition of nutrient solution can be completed in a sealed environment, avoiding oxygen from entering and causing anaerobic mycelium contamination, thereby ensuring the normal growth of anaerobic bacteria.
[0021] In the present application, a plurality of drippers 7 are fixedly connected to the bottom side of the spray plate 5, and the plurality of drippers 7 are evenly distributed in a ring shape; by arranging the plurality of drippers 7 to be evenly distributed in a ring shape, the nutrient solution can be evenly dripped into the culture dish 6, thereby avoiding differences in the degree of growth of mycelium in different areas due to different amounts of nutrient solution added.
[0022] In the present application, a spring 8 is fixedly connected to the inner wall of the bottom side of the columnar groove, and the top of the spring 8 is fixedly connected to the bottom side of the sealing block 3; by setting the spring 8, when the nutrient solution is injected, the sealing block 3 is squeezed and moves downward. At this time, the spring 8 can be used to synchronously drive the sealing block 3 to move upward and reset after the nutrient solution is injected to achieve sealing of the L-shaped tube 4, thereby further preventing oxygen from entering the sealing cover 2.
[0023] In the present application, a sealing rubber ring 9 is fixedly connected to the top side of the base 1, and an annular sealing groove is provided on the bottom side of the sealing cover 2, and the sealing rubber ring 9 is in contact with the inner wall of the annular sealing groove; by providing the sealing rubber ring 9 and the annular sealing groove, when the sealing cover 2 is in contact with the base 1, the sealing rubber ring 9 and the annular sealing groove cooperate to further improve the airtightness between the sealing cover 2 and the base 1, thereby achieving the effect of completely blocking the entry of oxygen.
[0024] In the present application, a plurality of electric heating rods 10 are fixedly installed on the inner side of the sealing cover 2, and the plurality of electric heating rods 10 are electrically connected to an external power source; by setting the electric heating rods 10, the interior of the sealing cover 2 can be heated by the electric heating rods 10, thereby providing a growth environment with a suitable temperature for anaerobic bacteria.
[0025] The standard parts used in the utility model can all be purchased from the market, and special-shaped parts can be customized according to the instructions and the drawings. The specific connection methods of each part adopt the conventional means such as mature bolts, rivets, welding, etc. in the existing technology. The machinery, parts and equipment all adopt the conventional models in the existing technology, and the circuit connection adopts the conventional connection method in the existing technology, which will not be described in detail here.
[0026] The implementation principle of an anaerobic bacteria cultivation box in the embodiment of the present application is as follows: when in use, the culture dish 6 needs to regularly add nutrient solution when cultivating anaerobic bacteria. When adding nutrient solution, the external nutrient solution injection tube is squeezed against the sealing block 3. As the sealing block 3 gradually moves downward, the external nutrient solution injection tube will be connected with the two L-shaped tubes 4. The nutrient solution will enter the interior of the spray plate 5 along the L-shaped tube 4 and drip into the culture dish 6, so that the addition of nutrient solution can be completed in a sealed environment, avoiding oxygen entry and causing anaerobic mycelium contamination, thereby ensuring the normal growth of anaerobic bacteria. The multiple drippers 7 are equidistantly distributed in a ring shape, so that the nutrient solution can be evenly dripped into the culture dish 6 to avoid In order to avoid the difference in the degree of mycelium growth in different areas due to the different amount of nutrient solution added, when the nutrient solution is injected, the sealing block 3 will be squeezed and move downward. At this time, the spring 8 can be used to synchronously drive the sealing block 3 to move upward and reset after the nutrient solution is injected to achieve the blocking of the L-shaped tube 4, thereby further preventing oxygen from entering the sealing cover 2. When the sealing cover 2 is fitted with the base 1, the sealing rubber ring 9 and the annular sealing groove can cooperate to further improve the airtightness between the sealing cover 2 and the base 1, so as to completely block the entry of oxygen. The electric heating rod 10 can be used to heat the inside of the sealing cover 2, thereby providing a suitable temperature growth environment for anaerobic bacteria.
[0027] Finally, a few points should be explained: First, in the description of this application, it should be noted that, unless otherwise specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense, which may refer to mechanical connection or electrical connection, or internal communication between two components, or direct connection. "upper", "lower", "left", "right", etc. are only used to indicate relative positional relationships. When the absolute position of the object being described changes, the relative positional relationship may change;
[0028] Secondly: The drawings of the embodiments disclosed in the present utility model only involve structures related to the embodiments disclosed in the present utility model, and other structures can refer to the general design. In the absence of conflict, the same embodiment and different embodiments of the present utility model can be combined with each other;
[0029] Finally: The above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present utility model should be included in the protection scope of the present utility model.
Claims
1. An anaerobic bacteria cultivation box, comprising a base (1), characterized in that: A sealing cover (2) is adapted to be placed on the top side of the base (1), a columnar groove is provided on the top side of the sealing cover (2), a sealing block (3) is slidably connected to the inner wall of the columnar groove, the sealing block (3) is in contact with the inner wall of the columnar groove, the left and right sides of the columnar groove are fixedly connected to L-shaped tubes (4), a spray plate (5) is fixedly connected to the inner wall of the top side of the sealing cover (2), the top side of the spray plate (5) is fixedly connected to the two L-shaped tubes (4), and a culture dish (6) is placed on the top side of the base (1).
2. The anaerobic bacteria cultivation box according to claim 1, characterized in that: The bottom side of the spray plate (5) is fixedly connected to a plurality of drippers (7), and the plurality of drippers (7) are distributed in a ring shape and at equal intervals.
3. The anaerobic bacteria cultivation box according to claim 1, characterized in that: A spring (8) is fixedly connected to the inner wall of the bottom side of the columnar groove, and the top end of the spring (8) is fixedly connected to the bottom side of the sealing block (3).
4. The anaerobic bacteria cultivation box according to claim 1, characterized in that: A sealing rubber ring (9) is fixedly connected to the top side of the base (1), an annular sealing groove is provided on the bottom side of the sealing cover (2), and the sealing rubber ring (9) is in contact with the inner wall of the annular sealing groove.
5. The anaerobic bacteria cultivation box according to claim 1, characterized in that: A plurality of electric heating rods (10) are fixedly mounted on the inner side of the sealing cover (2), and the plurality of electric heating rods (10) are all electrically connected to an external power source.