Chiral configuration demonstration experiment instrument based on circular dichroism spectrum

Abstract

The utility model discloses a chiral configuration demonstration experiment instrument based on a circular dichroism spectrum, and relates to the technical field of teaching aids. The device comprises a substrate, a plurality of mounting seats, a reflecting mirror, a polarizing film, a 1 / 4 plectrum, a sample pool, a triangular prism and a CCD (Charge Coupled Device) camera, wherein the plurality of mounting seats are respectively provided with a parallel light source emitter and a slit; a limiting plate is elastically matched in the base plate, a first hand screw is in threaded fit with the side face of the base plate, a plurality of first inserting grooves are formed in the base plate in the vertical direction, and second inserting grooves corresponding to the first inserting grooves are formed in the limiting plate in the vertical direction. The mounting base comprises an inserting block clamped in the first inserting groove, and a clamping groove corresponding to the limiting plate is formed in the side face of the inserting block. According to the utility model, the first slot is matched with the second slot, so that the insertion block can be conveniently clamped on the base plate, and the limiting plate elastically slides to be matched with the first hand screw and the clamping slot, so that the first hand screw can be conveniently screwed to limit and release the limit of the plurality of mounting seats at the same time.

Description

Technical Field

[0001] The utility model belongs to the field of teaching aids, and in particular relates to a chiral configuration demonstration experimental instrument based on circular dichroism spectrum. Background Art

[0002] In the teaching of physical optics, it is necessary to explain the reflection and refraction of light. The traditional approach is to draw diagrams and use straight lines to represent the propagation path of light. Most of the methods are to lecture verbatim, which is relatively abstract and difficult to understand. The learners cannot dynamically experience the changes in light color, which affects the teaching effect. Optical teaching aids are teaching equipment used for demonstration.

[0003] In existing optical teaching aids, the H-shaped pressure plate at the bottom of the rod bracket is usually installed on the breadboard using a hexagon socket head screw, and the hexagon socket head screw needs to be tightened to press the head of the H-shaped pressure plate to fix it. The operation process is relatively cumbersome, time-consuming and labor-intensive. Utility Model Content

[0004] The technical problem to be solved by the utility model is to overcome the deficiencies of the prior art and provide a chiral configuration demonstration experimental instrument based on circular dichroism spectroscopy.

[0005] In order to solve the above technical problems, the basic concept of the technical solution adopted by the utility model is:

[0006] A chiral configuration demonstration experimental instrument based on circular dichroism spectrum, comprising a substrate, a plurality of mounting seats, wherein the plurality of mounting seats are respectively provided with a parallel light source emitter and a slit, a reflector, a polarizer, a 1 / 4 pick, a sample cell, a prism, and a CCD camera;

[0007] The base plate is elastically matched with a limit plate, and the side thread is matched with a first hand-tightened screw corresponding to the limit plate. The base plate is vertically provided with a plurality of first slots, the first slots penetrate the base plate, and the limit plate is vertically provided with a second slot corresponding to the first slot, the second slot penetrates the limit plate;

[0008] The mounting base includes an insert block that is snap-fitted into a first slot, the insert block vertically passes through a second slot corresponding thereto, a slot corresponding to the limit plate is provided on a side of the insert block, the slot passes through the insert block in a transverse direction, a rotary lock assembly is rotatably engaged at the top of the insert block, and a fixed assembly is slidably engaged at the top of the rotary lock assembly.

[0009] Optionally, a movable groove corresponding to the limit plate is provided in the base plate, the limit plate slides in the movable groove, the first slot vertically penetrates the movable groove, a first screw hole corresponding to the first hand screw is provided on the side of the base plate, the first screw hole is connected to the movable groove, and the first hand screw thread is engaged in the first screw hole.

[0010] Optionally, side grooves are provided on both sides of the inner wall of the movable groove, the first screw hole is located between the two side grooves, extrusion blocks are provided on both sides of the limiting plate, the extrusion blocks are slidably fitted in the side grooves, a first spring is provided between one side of the extrusion block and one side of the inner wall of the side groove, and the extrusion block is located between the first spring and the first hand-tightening screw.

[0011] Optionally, the rotary lock assembly includes a rotating plate that rotatably cooperates with the upper side of the plug block, a first slot is provided on the lower side of the rotating plate, an extension column located in the first slot is installed on the upper side of the plug block, a rotating slot is provided at the upper end of the inner wall of the first slot, a gear located in the rotating slot is installed at the upper end of the extension column, a locking slot is provided on the side of the inner wall of the rotating slot, an arc-shaped rack is slidably fitted in the lock slot, a second spring is provided between the side of the arc-shaped rack and one side of the inner wall of the lock slot, the arc-shaped rack is located between the gear and the second spring, a second slot connected to the lock slot is provided on the side of the rotating plate, a rod body is provided on the side of the arc-shaped rack, the second spring is sleeved on the circumferential side of the rod body, the rod body passes through the second slot in the transverse direction, and a pull tab is provided on the end of the rod body away from the arc-shaped rack, and the pull tab is located on the side of the rotating plate.

[0012] Optionally, the fixing assembly includes an installation tube, a sliding column is installed at the lower end of the installation tube, a sliding groove corresponding to the sliding column is provided on the upper side of the rotating plate, the sliding column slides in the sliding groove, and a second hand-tightening screw is rotatably engaged on the rotating plate. The second hand-tightening screw passes through the sliding groove in the horizontal direction, the sliding column thread is engaged on the circumferential side of the second hand-tightening screw, a second screw hole is provided on the side of the installation tube, and the inner thread of the second screw hole is engaged with a third hand-tightening screw.

[0013] Optionally, grooves are provided on both sides of the inner wall of the slide groove, a sliding block slidably fitted in the groove is installed on the side of the slide column, and the second hand screw is located between the two sliding blocks.

[0014] After adopting the above technical solution, the utility model has the following beneficial effects compared with the prior art. Of course, any product implementing the utility model does not necessarily need to achieve all the advantages described below at the same time:

[0015] The first slot cooperates with the second slot to facilitate the insertion block to be connected to the base plate, and the limiting plate elastically slides and cooperates with the first hand screw and the slot to facilitate the first hand screw to limit and release the limit of multiple mounting seats at the same time, thereby improving the convenience of using the teaching aid.

[0016] The specific implementation of the utility model is further described in detail below with reference to the accompanying drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] The drawings described below are only some embodiments. For those skilled in the art, other drawings can be obtained based on these drawings without creative work. In the drawings:

[0018] Figure 1This is a schematic diagram of the three-dimensional structure of an embodiment of the utility model;

[0019] Figure 2 This is a schematic diagram of a cross-sectional structure of a substrate according to an embodiment of the utility model;

[0020] Figure 3 This is a schematic cross-sectional structure diagram of a rotary lock assembly according to an embodiment of the utility model;

[0021] Figure 4 This is a schematic cross-sectional structure diagram of a fixing assembly according to an embodiment of the utility model;

[0022] Figure 5 A schematic diagram of a circular dichroism spectrum of an embodiment of the utility model;

[0023] Figure 6 A table showing the correspondence between peaks and pixel values ​​according to an embodiment of the present invention;

[0024] Figure 7 Schematic diagram of the absorption spectrum test result (left) and standard result (right) of an empty sample cell according to an embodiment of the utility model;

[0025] Figure 8 Schematic diagram of the absorption spectrum test result (left) and standard result (right) of a chiral sample solution according to an embodiment of the present invention;

[0026] Fig. 9 The circular dichroism spectra of two configurations of arginine in one embodiment of the utility model are shown;

[0027] Fig.10 This is a standard circular dichroism spectrum of arginine according to an embodiment of the present invention.

[0028] In the accompanying drawings, the components represented by the reference numerals are listed as follows:

[0029] Base plate 1, movable slot 101, side slot 102, first slot 103, first screw hole 104, limit plate 2, extrusion block 201, second slot 202, first spring 203, first hand screw 3, plug block 4, card slot 401, extension column 402, gear 403, rotating plate 5, slide slot 501, channel 502, first slot hole 503, rotating slot 504, locking slot 505, second slot hole 506, arc rack 6, rod body 601, pull tab 602, second spring 603, second hand screw 7, mounting tube 8, second screw hole 801, slide column 802, slider 803, third hand screw 9, parallel light source emitter 10, slit 11, reflector 12, polarizer 13, 1 / 4 pick 14, sample cell 15, prism 16, CCD camera 17.

[0030] It should be noted that these drawings and textual descriptions are not intended to limit the conceptual scope of the present invention in any way, but rather to illustrate the concept of the present invention for those skilled in the art by referring to specific embodiments. DETAILED DESCRIPTION

[0031] The utility model will now be described in further detail with reference to the accompanying drawings.

[0032] See also Figure 1-10 As shown, in this embodiment, a chiral configuration demonstration experimental instrument based on circular dichroism spectroscopy is provided, including a substrate 1, a plurality of mounting seats, and a parallel light source emitter 10 and a slit 11, a reflector 12, a polarizer 13, a 1 / 4 pick 14, a sample cell 15, a prism 16, and a CCD camera 17 are respectively arranged on the plurality of mounting seats;

[0033] The base plate 1 is elastically matched with the limit plate 2, and the side thread is matched with the first hand screw 3 corresponding to the limit plate 2. The base plate 1 is vertically provided with a plurality of first slots 103, and the first slots 103 penetrate the base plate 1. The limit plate 2 is vertically provided with second slots 202 corresponding to the first slots 103, and the second slots 202 penetrate the limit plate 2.

[0034] The mounting base includes an insert block 4 that is snap-fitted into the first slot 103. The insert block 4 vertically penetrates the corresponding second slot 202. A slot 401 corresponding to the limit plate 2 is provided on the side of the insert block 4. The slot 401 penetrates the insert block 4 in the horizontal direction. The upper part of the insert block 4 is rotatably engaged with a rotary lock component, and the upper part of the rotary lock component is slidably engaged with a fixed component.

[0035] One aspect of the application of this embodiment is: when in use, the card slot 401 of the plug block 4 is inserted into the first slot 103 and the corresponding second slot 202 in the direction of the first hand screw 3. After the required number of mounting seats are installed, the first hand screw 3 is screwed into the base plate 1 to make the first hand screw 3 squeeze the limit plate 2 to move, so that the second slot 202 is misaligned with the first slot 103, and part of the structure of the limit plate 2 is inserted into the card slot 401, thereby completing the fixed limit of multiple plug blocks 4 at the same time, and then the parallel light source emitter 10 and the slit 11, the reflector 12, the polarizer 13, the 1 / 4 pick 14, the sample cell 15, the prism 16, and the CCD camera 17 are installed on the fixed component through a column or an optical adjustment frame, and then the rotary lock component is rotated to adjust the handover, and the position of the fixed component on the rotary lock component is adjusted for testing.

[0036] In the present embodiment, a movable groove 101 corresponding to the limit plate 2 is provided in the base plate 1, and the limit plate 2 is slidably fitted in the movable groove 101. The first slot 103 vertically penetrates the movable groove 101. A first screw hole 104 corresponding to the first hand screw 3 is provided on the side of the base plate 1. The first screw hole 104 is connected to the movable groove 101, and the first hand screw 3 is threadedly fitted in the first screw hole 104. The displacement direction and displacement distance of the limit plate 2 are limited by the movable groove 101.

[0037] In this embodiment, side grooves 102 are provided on both sides of the inner wall of the movable groove 101, and the first screw hole 104 is located between the two side grooves 102. Extrusion blocks 201 are provided on both sides of the limiting plate 2. The extrusion blocks 201 are slidably fitted in the side grooves 102. A first spring 203 is provided between one side of the extrusion block 201 and one side of the inner wall of the side groove 102. The extrusion block 201 is located between the first spring 203 and the first hand-tightening screw 3. When the first hand-tightening screw 3 is loosened, the first spring 203 rebounds and pushes the extrusion block 201, so that the limiting plate 2 is quickly reset.

[0038] The rotary lock assembly of this embodiment includes a rotating plate 5 that is rotatably matched with the upper side of the plug block 4, a first slot hole 503 is provided on the lower side of the rotating plate 5, an extension column 402 located in the first slot hole 503 is installed on the upper side of the plug block 4, a rotating groove 504 is provided on the upper end of the inner wall of the first slot hole 503, a gear 403 located in the rotating groove 504 is installed on the upper end of the extension column 402, a locking groove 505 is provided on the side of the inner wall of the rotating groove 504, an arc-shaped rack 6 is slidably matched in the locking groove 505, the inner wall of the arc-shaped rack 6 is meshed with the gear 403, a second spring 603 is provided between the side of the arc-shaped rack 6 and one side of the inner wall of the locking groove 505, the arc-shaped rack 6 is located between the gear 403 and the second spring 603, a second slot hole 506 connected to the locking groove 505 is provided on the side of the rotating plate 5, and a The rod body 601 and the second spring 603 are sleeved on the circumferential side of the rod body 601. The rod body 601 passes through the second slot 506 in the horizontal direction. A pull tab 602 is installed at the end of the rod body 601 away from the arc-shaped rack 6. The pull tab 602 is located on the side of the rotating plate 5. When the angle of the rotating plate 5 needs to be adjusted, the pull tab 602 is pulled to drive the rod body 601 and the arc-shaped rack 6 to move. The displacement of the arc-shaped rack 6 cancels the meshing state with the gear 403 and squeezes the second spring 603. When the rotating plate 5 rotates to the specified angle, the pull tab 602 is released, and the second spring 603 rebounds to push the arc-shaped rack 6 to quickly reset, thereby re-fixing the rotation of the rotating plate 5. The pull tab 602 cooperates with the arc-shaped rack 6 and the gear 403 to improve the convenience of adjusting and fixing the angle of the rotating plate 5.

[0039] The fixing assembly of this embodiment includes an installation tube 8, a sliding column 802 is installed at the lower end of the installation tube 8, and a sliding groove 501 corresponding to the sliding column 802 is provided on the upper side of the rotating plate 5, and the sliding column 802 is slidably matched in the sliding groove 501, and a second hand screw 7 is rotatably matched on the rotating plate 5, and the second hand screw 7 passes through the sliding groove 501 in the horizontal direction, and the sliding column 802 is threadedly matched on the peripheral side of the second hand screw 7, and a second screw hole 801 is provided on the side of the installation tube 8, and the inner thread of the second screw hole 801 is matched with a third hand screw 9. During installation, the lower part of the column is inserted into the installation tube 8, and then the third hand screw 9 is rotated to make it contact with the side of the column. When it is necessary to adjust the position of the installation tube 8 on the rotating plate 5, the second hand screw 7 is rotated to drive the sliding column 802 to slide along the sliding groove 501, and the third hand screw 9 cooperates with the sliding column 802, so as to facilitate the adjustment of the position of the installation tube 8 and improve the accuracy of the adjustment of the installation tube 8.

[0040] In this embodiment, grooves 502 are provided on both sides of the inner wall of the slide groove 501, and a slider 803 that slides in the groove 502 is installed on the side of the slide column 802. The second hand screw 7 is located between the two sliders 803. The slider 803 cooperates with the groove 502 to improve the stability of the slide column 802 when sliding.

[0041] 1. The experimental principle of this teaching aid:

[0042] Polarized light: Light is a transverse electromagnetic wave, a ray that vibrates in all directions. Its electric field vector E and magnetic field vector H are perpendicular to each other and to the propagation direction of the light wave. Since the electric field vector is the main factor that produces photosensitivity, the electric field vector is generally used as the vibration vector of the light wave. The plane formed by the electric field vector of the light wave and the propagation direction is called the vibration plane of the light wave. If this vibration plane does not change with time, is limited to a fixed direction, and does not vibrate in all planes, this beam of light is called plane polarized light, and its vibration plane is called the polarization plane.

[0043] Circularly polarized light and elliptically polarized light: Plane polarized light can be decomposed into two circularly polarized light beams with the same amplitude and frequency and opposite rotation directions. The one with the electric vector rotating in the clockwise direction is called right-hand circularly polarized light, and the one with the electric vector rotating in the counterclockwise direction is called left-hand circularly polarized light. Two beams of polarized light with the same amplitude and frequency and opposite rotation directions can also be combined into a beam of plane polarized light. If the amplitude (intensity) of the two polarized light beams is different, the synthesized light will be elliptically polarized light.

[0044] Optical activity: If an organic molecule is chiral, when circularly polarized light passes through it, the polarization plane will rotate. The light enters one plane in a wave shape and is emitted from another plane in a wave shape. That is, the substance has "optical activity";

[0045] Circular dichroism: The molar absorptivity of the left-handed circularly polarized light and the right-handed circularly polarized light that constitute the plane polarized light of the optically active organic molecule is different, that is, IL≠IR, and the difference in light absorption ΔA=I L -I D w is called the "circular dichroism" (CD) of the substance;

[0046] Circular dichroism spectrum: The difference between the two molar absorption coefficients ΔA is also called CD, which changes with the length of the incident polarized light. With ΔA as the ordinate and wavelength as the horizontal scale, the resulting spectrum is the "circular dichroism spectrum", such as Figure 5 shown.

[0047] 2. Experimental steps and device construction:

[0048] Step 1: Install the parallel light source transmitter 10 and the slit 11, the reflector 12, the polarizer 13, the 1 / 4 pick 14, the sample cell 15, the prism 16, and the CCD camera 17, and adjust all the equipment to the same height. According to the spectral properties of the prism 16, the spectral direction is roughly determined. First, the CCD camera 17 is adjusted to be roughly oriented in this direction, and then the direction is slightly adjusted so that the large light spot generated by the parallel light source transmitter 10 is located in the center of the CCD camera 17. The slit 11 is installed, and the focal length of the camera and the angle of the prism 16 are fine-tuned so that the CCD camera 17 can capture a clearer spectrum.

[0049] Step 2: At this time, the data obtained from the camera is the relationship between light intensity and pixel position. Since the wavelength relationship needs to be obtained, it is necessary to establish a mapping from pixel position to wavelength. Remove the parallel light source emitter 10, install a mercury lamp behind the slit 11, use a CCD camera 17 to shoot the mercury lamp emission spectrum image, use MATLAB to read the brightness of each pixel position, and obtain the mercury lamp intensity-pixel position curve. In the spectrum diagram, the pixel values ​​corresponding to the four peaks in the visible light range are obtained. According to the relevant knowledge of electron transition, the electrons in the excited state in the mercury lamp will emit photons with energy equal to the energy difference between the two high and low orders when jumping back to the low energy level. Then according to the formula:

[0050] E = hνλ;

[0051] λ = c / ν;

[0052] The wavelengths of light emitted by the mercury lamp can be obtained. The corresponding pixel values ​​corresponding to the peaks in the visible light range are as follows: Figure 6 As shown, the linear fitting function FIT is used in MATLAB to fit the pixel position and wavelength, and the corresponding functional relationship between the actual wavelength and the pixel position in the photo taken by the camera is obtained as follows:

[0053] λ = 0.6139x - 341.2;

[0054] Step 3: To detect whether each component is operating normally and test the accuracy of the output spectrum, the absorption spectrum of the sample can be measured and compared with the standard. Without adding a sample, the empty sample pool 15 is directly measured to obtain the light intensity curve Ibase as a base, and a DCM (dichloromethane)-methanol solution is injected into the sample pool 15. Since the solution is volatile, the sample pool 15 is closed with an elastic bandage to prevent volatilization, and the light intensity curve IDCM is measured, using the formula:

[0055]

[0056] In order to eliminate the influence of background on the absorption spectrum, the final absorption spectrum (such as Figure 7 The peak value of the light spectrum of the chiral sample solution is about 465nm, and the wavelength range is about 400nm-530nm, which is consistent with the standard (peak value about 460nm, range 400nm-530nm). Therefore, the device can measure the absorption spectrum of the substance more accurately, which lays a reliable foundation for the measurement of circular dichroism spectrum. At the same time, the absorption spectrum of the chiral sample solution is measured. Compared with the results of scientific research instruments, the absorption peak located at 450nm-500nm, which is within the credible section of the light source, is accurately measured.

[0057] Step 4: Amino acid is the basic unit of protein, which gives protein a specific molecular structure and makes its molecule biochemically active. Protein constitutes the main structure of organisms, and most of the catalytic enzymes are also composed of proteins. Except for glycine, amino acids in nature are chiral and L-type. In addition, the price of amino acids is low and the cost is low. Therefore, selecting amino acids as test samples has strong practical significance and feasibility;

[0058] Arginine (Arg) is one of the most widely used amino acids in animal cells. It is not only a precursor for protein synthesis, but also a precursor for the synthesis of nitric oxide, urea, polyamines, proline, glutamic acid, creatine and herring spermine. Therefore, arginine was selected as the chiral sample.

[0059] However, when amino acids are directly used as circular dichroism detection samples, the theoretical peak range of their circular dichroism spectra is in the ultraviolet light section of 180-210nm, and the parallel light source used is LED light, which cannot meet the requirements. Therefore, the properties of tetraphenylporphyrin tetrasulfonic acid (TPPS) can be used. TPPS itself has no chirality, but TPPS can self-assemble and aggregate to form a chiral structure. Arginine is added to the TPPS solution. The L configuration of arginine can induce TPPS to form an L-type chiral structure, and the D-configuration amino acid can induce TPPS to form a D-type chiral structure. However, the color of TPPS itself is 420nm, which is close to the edge of the wavelength range of the selected light source. Adding ionic liquid C4BF4 can promote the self-assembly aggregation of TPPS and shift the absorption peak to around 490nm, which is closer to the confidence interval of the light source, making the measurement result more accurate. Therefore, an arginine-TPPS-C4BF4 mixed solution is selected as the chiral solvent;

[0060] Step 5: Without adding a sample, the 1 / 4 pick 14 is rotated 45° to the left to directly measure the intensity of the left-handed polarized light, and then rotated 45° to the right to measure the intensity of the right-handed polarized light. The CD of this intensity is used as the base to obtain the circular dichroism spectrum of the sample. It is necessary to subtract the base for calibration to minimize the interference of environmental factors. The calculation formula of circular dichroism ΔA, i.e. CD, is:

[0061]

[0062] Subsequently, D-arginine was injected into the sample tank and the same measurement was performed and CD was calculated. To avoid interference from residues, L-arginine was used for three rinses and then L-arginine was injected for the same measurement. The circular dichroism spectra of D-type and L-type were obtained respectively. For ease of analysis, MATLAB was used to simultaneously plot the curves of L-type, D-type and the substrate. It can be seen that for substances that are chirally symmetrical to each other, their circular dichroism spectra are roughly symmetrical along the baseline.

[0063] 3. Circular dichroism spectroscopy analysis:

[0064] If I D >I L , it means that the intensity of right-handed polarized light after passing through the sample is greater than that of left-handed polarized light, then On the contrary, if the left-handed light is stronger than the right-handed light, then CD<0. In the circular dichroism spectrum, if CD>0, the image is above the baseline; if CD<0, the image is below the baseline. Based on the properties of circular dichroism of chiral substances and the standard circular dichroism spectrum, the configuration of the sample to be tested can be analyzed.

[0065] For an unknown chiral arginine sample, we only need to prepare its TPPS-C4BF4 solution, use the device to measure its circular dichroism spectrum according to the steps, and compare it with the spectrum of the standard sample. If the spectrum line of the unknown sample is roughly close to the yellow line, it is D configuration, otherwise it is close to the red line, it is L configuration;

[0066] By extension, for any standard chiral sample solution whose circular dichroic spectrum peak is within the confidence wavelength range of the light source, the configuration of the chiral sample to be tested can be determined based on this and the above method.

[0067] 4. Conclusion:

[0068] Compared with the circular dichroism spectrum measured by scientific research instruments, the device can observe that the peak of the circular dichroism spectrum near 450nm-500nm is measured more accurately. The device can use the circular dichroism spectrum to more accurately determine the chirality of chiral substances. The device realizes the basic detection function of the circular dichroism spectrometer for scientific research at a relatively low cost, and also realizes the function of the absorption spectrometer. The device can be used as a feasible replacement for related scientific research detection instruments. Under the conditions that the accuracy requirement is not at the scientific research level or the cost is limited, the device can well give the circular dichroism spectrum of chiral substances and realize the chiral configuration determination.

[0069] Experimental results: The experimental device can measure the circular dichroism spectrum of the sample more accurately and verify the chirality of the sample through it;

[0070] Demonstration effect: the obtained spectrum is clear and accurate, highly reliable, intuitive and easy to understand;

[0071] Operational process: The experimental operation is relatively simple. After the CCD reads the data, the MATLAB script automatically processes the data and outputs the results, which is convenient for experimental demonstration and teaching.

[0072] The device also has the following features: the experimental process is simple and reliable, and the data is automatically processed by MATLAB scripts; it does not have high requirements on sample concentration / purity / functional group / crystallization and other properties; it uses an adjustable wave plate, which can be adjusted directly to avoid repeated removal of the wave plate, and a slit / sample separation design to facilitate sample replacement and minimize the impact on the optical path; it uses multiple measurements and averaging to improve the signal-to-noise ratio; in addition to circular dichroism spectra, it can also obtain ordinary spectra, realizing multiple uses of one device;

[0073] In addition, chirality has always been an indispensable part of the teaching of related biology, chemistry, and physics in junior high school, high school, or university. Mutually chiral substances often have basically no difference in chemical properties, and the differences in the physical level are also relatively microscopic, which is difficult to show macroscopically. Compared with scientific research instruments that cost hundreds of thousands of yuan, this device is more suitable as a teaching aid in the classroom due to its low cost. The circular dichroism spectrum also realizes the macroscopic difference of chiral substances, which is expected to provide new ideas and methods for the teaching of chirality-related knowledge.

[0074] At the same time, as a physical experiment teaching aid, the device combines physical optics knowledge with biochemical chirality knowledge to form an interdisciplinary approach. It can train students to use optical instruments and components to conduct optical experiments while supplementing chirality-related knowledge. Experiments based on optical flat panels are often the basis of optical experiments, while MATLAB operations and CCD computer interactions are also an indispensable part of physical experiments. Familiarity with this experiment can lay the foundation for subsequent related scientific research.

[0075] The present invention is not limited to the above-mentioned implementation modes. Anyone should be aware of the structural changes made under the inspiration of the present invention. Any technical solution that is the same or similar to the present invention falls within the protection scope of the present invention. The technology, shape, and structure that are not described in detail in the present invention are all known technologies.

Claims

1. A chiral configuration demonstration experimental instrument based on circular dichroism spectroscopy, characterized in that: include: A substrate (1), a plurality of mounting seats, wherein the plurality of mounting seats are respectively provided with a parallel light source emitter (10) and a slit (11), a reflector (12), a polarizing plate (13), a 1 / 4 pick (14), a sample pool (15), a prism (16), and a CCD camera (17); The base plate (1) is elastically matched with a limit plate (2), and the side thread is matched with a first hand screw (3); the base plate (1) is vertically provided with a plurality of first slots (103), and the limit plate (2) is vertically provided with second slots (202) corresponding to the first slots (103); The mounting seat comprises an insert block (4) which is snap-fitted into the first slot (103); a slot (401) corresponding to the limit plate (2) is provided on the side of the insert block (4); a rotary lock component is rotatably engaged on the upper part of the insert block (4); and a fixing component is slidably engaged on the upper part of the rotary lock component.

2. The chiral configuration demonstration experimental instrument based on circular dichroism spectroscopy according to claim 1, characterized in that: A movable groove (101) corresponding to the limiting plate (2) is provided in the base plate (1), and a first screw hole (104) corresponding to the first hand-tightening screw (3) is provided on the side surface of the base plate (1).

3. The chiral configuration demonstration experimental instrument based on circular dichroism spectrum according to claim 2, characterized in that: Side grooves (102) are provided on both sides of the inner wall of the movable groove (101), and extrusion blocks (201) are provided on both sides of the limiting plate (2). The extrusion blocks (201) are slidably fitted in the side grooves (102), and a first spring (203) is provided between the extrusion blocks (201) and one side of the inner wall of the side groove (102).

4. The chiral configuration demonstration experimental instrument based on circular dichroism spectrum according to claim 1, characterized in that: The rotary lock assembly comprises a rotating plate (5) rotatably engaged with the upper side of the plug block (4), a first slot hole (503) is provided on the lower side of the rotating plate (5), an extension column (402) located in the first slot hole (503) is installed on the upper side of the plug block (4), a rotating groove (504) is provided on the upper end of the inner wall of the first slot hole (503), a gear (403) located in the rotating groove (504) is installed on the upper end of the extension column (402), and a lock groove (505) is provided on the side of the inner wall of the rotating groove (504). ), an arc-shaped rack (6) is slidably fitted in the lock groove (505), a second spring (603) is arranged between the arc-shaped rack (6) and one side of the inner wall of the lock groove (505), a second slot hole (506) connected to the lock groove (505) is arranged on the side of the rotating plate (5), a rod body (601) is arranged on the side of the arc-shaped rack (6), the rod body (601) passes through the second slot hole (506) in the horizontal direction, and a pull tab (602) is arranged on one end of the rod body (601).

5. The chiral configuration demonstration experimental instrument based on circular dichroism spectrum according to claim 4, characterized in that: The fixing assembly comprises a mounting tube (8), a sliding column (802) is mounted at the lower end of the mounting tube (8), a sliding groove (501) corresponding to the sliding column (802) is arranged on the upper side of the rotating plate (5), a second hand screw (7) is rotatably engaged on the rotating plate (5), the sliding column (802) is threadedly engaged on the circumference of the second hand screw (7), and a third hand screw (9) is threadedly engaged on the mounting tube (8).

6. The chiral configuration demonstration experimental instrument based on circular dichroism spectrum according to claim 5, characterized in that: Grooves (502) are provided on both sides of the inner wall of the slide groove (501), and a sliding block (803) which is slidably matched in the groove (502) is installed on the side of the slide column (802).

Images