Portable multichannel fluorescent nucleic acid detector

Abstract

The utility model relates to the technical field of fluorescent nucleic acid detectors, in particular to a portable multichannel fluorescent nucleic acid detector, which comprises a shell, a circuit board, a reaction tube and a fluorescent detection module, an installation cavity is arranged in the shell, one end of the installation cavity is communicated with a first opening, the circuit board is arranged in the installation cavity, and the reaction tube is arranged in the circuit board. A single chip microcomputer is installed on the circuit board, the fluorescence detection module comprises a spectrum sensor installed on the circuit board, the spectrum sensor is in signal connection with the single chip microcomputer, the fluorescence detection module further comprises an excitation light assembly, the reaction tube is installed in the excitation light assembly, and the excitation light assembly is in signal connection with the single chip microcomputer. The excitation light assembly excites a fluorescence signal of the fluorescent dye in the reaction tube, and the spectrum sensor is used for collecting the fluorescence signal. According to the multi-channel fluorescence detector, multi-channel fluorescence detection can be realized through one spectrum sensor, so that the whole size of the fluorescence detector is smaller, the production cost is lower, and the carrying is convenient.

Description

Technical Field

[0001] The utility model relates to the technical field of fluorescent nucleic acid detectors, and in particular to a portable multi-channel fluorescent nucleic acid detector. Background Art

[0002] As a high-throughput, low-cost technical means, multiple fluorescence signal detection is widely used in large-scale screening and quantitative detection of pathogens, especially in the field of nucleic acid detection. With the help of different fluorescent dyes (such as FAM, ROX, Cy3, Cy5, etc.) to generate emission light signals of different wavelengths under the action of excitation light, it is possible to simultaneously monitor multiple targets to be detected in a single reaction. However, the PCR reaction relies on precise temperature control equipment to complete multiple cycles of denaturation, annealing, and extension reactions. The instrument is large in size, has high power consumption, and has a long reaction time. In recent years, emerging constant temperature nucleic acid detection technologies such as loop-mediated isothermal amplification (LAMP), recombinase polymerase amplification (RPA), and clustered regularly interspaced short palindromic repeats (CRISPR) can efficiently detect nucleic acid targets at a single specific temperature, greatly simplifying the structure of nucleic acid instruments, reducing instrument costs, and facilitating popularization and application.

[0003] The existing fluorescent nucleic acid detector includes a circuit board installed inside the shell, a detection chip (single-chip microcomputer) installed on the circuit board, a fluorescent excitation light source installed in the shell, an excitation light filter is arranged between the fluorescent excitation light source and the reaction tube, and a plurality of photoelectric detection elements (photoelectric detection elements are photodiodes or photomultiplier tubes, etc.) are also arranged in the shell, and emission light filters are arranged between the plurality of photoelectric detection elements and the reaction tube. After the sample is processed (pathogen lysis), it is poured into a reaction tube containing a detection system (including nucleic acid amplification primers, probes, enzymes and fluorescent dyes, etc.). At this time, the nucleic acid detection reagent in the reaction tube reacts, and the fluorescent excitation light source is filtered by the excitation light filter to excite the fluorescent signal of the fluorescent dye in the detection system in the reaction tube, and according to the set fluorescence detection channel, it is filtered by the emission light filter. Finally, the fluorescent signal can be collected by the photoelectric detection element and converted into an electrical signal. After amplification and filtering, the fluorescence intensity is quantified.

[0004] However, when the above-mentioned fluorescent nucleic acid detector realizes multi-channel fluorescence detection, in order to avoid cross-interference between channels, it is usually necessary to use multiple emission light filters of different wavelengths and multiple photoelectric detection elements to realize the filtering and collection of multiple fluorescence signals, which makes the overall size of the fluorescence detector larger, not portable and has high production cost. Utility Model Content

[0005] (I) The problem to be solved by the present invention is: how to provide a fluorescent nucleic acid detector which is small in size, portable and has low production cost.

[0006] (II) Technical solution

[0007] The utility model provides a portable multi-channel fluorescent nucleic acid detector, comprising: a shell, a circuit board, a reaction tube and a fluorescent detection module;

[0008] An installation cavity is provided in the shell, and one end of the installation cavity is connected to a first opening;

[0009] The circuit board is arranged in the installation cavity, a single-chip microcomputer is installed on the circuit board, the fluorescence detection module comprises a spectrum sensor installed on the circuit board, and the spectrum sensor is connected to the single-chip microcomputer by signal;

[0010] The fluorescence detection module also includes an excitation light component, the reaction tube is installed in the excitation light component, the excitation light component excites the fluorescence signal of the fluorescent dye in the reaction tube, and the spectrum sensor is used to collect the fluorescence signal.

[0011] According to an embodiment of the utility model, the excitation light assembly includes a combination plate and a fluorescent excitation light source, the combination plate is provided with a placement groove for placing the reaction tube at one end close to the first opening, the combination plate is provided with a light source mounting hole for placing the fluorescent excitation light source, and the light source mounting hole is connected to the placement groove;

[0012] The combination plate is also provided with a detection hole, the spectrum sensor is arranged at one end of the detection hole, and the other end of the detection hole is connected to the placement groove.

[0013] According to an embodiment of the utility model, a plurality of the fluorescent excitation light sources are provided, a plurality of the light source mounting holes are opened on the combination plate, and the plurality of the light source mounting holes correspond one-to-one to the plurality of the fluorescent excitation light sources.

[0014] According to an embodiment of the utility model, an excitation light filter is embedded in the light source installation hole, and the excitation light filter is located between the fluorescent excitation light source and the reaction tube.

[0015] According to an embodiment of the utility model, the combined plate includes a metal block and a mounting plate, and a slot is formed at one end of the mounting plate close to the first opening, and the metal block is fixedly connected in the slot.

[0016] According to an embodiment of the utility model, a first circular groove is formed on the metal block, a first through hole is formed on the mounting plate, and the first through hole is connected with the first circular groove to form the light source mounting hole;

[0017] The placement groove and the detection hole are both arranged on the metal block.

[0018] According to one embodiment of the utility model, a temperature control module is installed in the shell, and the portable multi-channel fluorescent nucleic acid detector also includes a lysis tube for sample processing. The temperature control module is used to detect and control the lysis temperature of the lysis tube and the reaction temperature of the reaction tube.

[0019] According to an embodiment of the utility model, the temperature control module includes a temperature sensor, a heating plate, a fan and a control unit, and the temperature sensor, the heating plate and the fan are all electrically connected to the control unit;

[0020] One side of the card slot is connected to a clearance groove, the heating plate is arranged in the clearance groove, the temperature sensor is installed on the metal block, and the fan is installed on a side of the combination board away from the circuit board.

[0021] According to an embodiment of the utility model, when the pathogen in the lysis tube is lysed to release nucleic acid, the control unit controls the heating plate to start until the temperature sensor detects that the lysis temperature in the lysis tube is equal to the first temperature threshold, the temperature sensor transmits the temperature signal to the control unit, and the control unit controls the temperature of the heating plate to maintain at the first temperature threshold for a period of time and then stops;

[0022] When the nucleic acid detection reagent in the reaction tube reacts, the control unit controls the fan to turn on until the temperature sensor detects that the reaction temperature in the reaction tube is equal to a preset second temperature threshold. The temperature sensor transmits a temperature signal to the control unit, and the control unit controls the fan to stop. After the control unit controls the temperature of the heating plate to maintain at the second temperature threshold for a period of time, the control unit controls the heating plate to stop.

[0023] According to an embodiment of the utility model, a first button and a second button are provided on the housing, and the first button and the second button are both electrically connected to the single chip computer;

[0024] The housing is provided with two indicator lights, and both of the two indicator lights are electrically connected to the single chip computer.

[0025] Beneficial effects of the utility model:

[0026] Specifically, the fluorescence signal of the fluorescent dye in the reaction tube is stimulated by the excitation light component, and the fluorescence signal is collected and recorded in real time by the spectral sensor. The spectral sensor can replace multiple emission light filters and multiple photoelectric detection elements, thereby making the overall size of the fluorescence detector smaller, the production cost lower, and easy to carry. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] In order to more clearly illustrate the specific implementation methods of the utility model or the technical solutions in the prior art, the drawings required for use in the specific implementation methods or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are some implementation methods of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0028] Figure 1 A three-dimensional diagram of a fluorescent nucleic acid detector provided by an embodiment of the utility model;

[0029] Figure 2 A first stereoscopic view of the interior of a housing provided by an embodiment of the utility model;

[0030] Figure 3 A second stereoscopic view of the interior of the housing provided by an embodiment of the utility model;

[0031] Figure 4 A three-dimensional diagram of a combined board provided in an embodiment of the utility model;

[0032] Figure 5 A three-dimensional disassembled diagram of a combined board provided in an embodiment of the utility model;

[0033] Figure 6 A three-dimensional cross-sectional view of a combined panel provided in an embodiment of the utility model.

[0034] Icons: 1. Shell; 101. Heat dissipation hole; 102. Shell cover; 103. Fixing plate; 2. Circuit board; 3. Combination board; 301. Metal block; 302. Mounting plate; 303. Placement slot; 304. Light source mounting hole; 305. Detection hole; 4. Fluorescence excitation light source; 5. Reaction tube; 6. Spectral sensor; 7. Fan; 8. Power interface; 9. Heating plate; 10. Indicator light; 11. First button; 12. Second button; 13. Temperature sensor. DETAILED DESCRIPTION

[0035] The technical solution of the utility model will be clearly and completely described below in conjunction with the embodiments. Obviously, the described embodiments are part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments of the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0036] like Figures 1 to 6 As shown, one embodiment of the utility model provides a portable multi-channel fluorescent nucleic acid detector, including: a housing 1, a circuit board 2, a reaction tube 5 and a fluorescent detection module;

[0037] An installation cavity is provided in the housing 1, and one end of the installation cavity is connected to a first opening;

[0038] The circuit board 2 is arranged in the installation cavity, a single chip microcomputer is installed on the circuit board 2, and the fluorescence detection module includes a spectrum sensor 6 installed on the circuit board 2, and the spectrum sensor 6 is connected to the single chip microcomputer signal;

[0039] The fluorescence detection module also includes an excitation light component. The reaction tube 5 is installed in the excitation light component. The excitation light component excites the fluorescence signal of the fluorescent dye in the reaction tube 5. The spectrum sensor 6 is used to collect the fluorescence signal.

[0040] Specifically, the fluorescence signal of the fluorescent dye in the reaction tube 5 is stimulated by the excitation light component, and the fluorescence signal is collected and recorded in real time by the spectral sensor 6. The spectral sensor 6 can replace multiple emission light filters and multiple photoelectric detection elements, thereby making the overall size of the fluorescence detector smaller, the production cost lower, and easy to carry.

[0041] The excitation light assembly includes a combination plate 3 and a fluorescent excitation light source 4. A placement groove 303 for placing the reaction tube 5 is provided at one end of the combination plate 3 close to the first opening. A light source mounting hole 304 for placing the fluorescent excitation light source 4 is provided on the combination plate 3. The light source mounting hole 304 is connected to the placement groove 303.

[0042] A detection hole 305 is also provided on the combined plate 3 . The spectrum sensor 6 is arranged at one end of the detection hole 305 . The other end of the detection hole 305 is connected to the placement groove 303 .

[0043] Specifically, the reaction tube 5 containing the nucleic acid detection reagent is placed in the placement slot 303, the fluorescent excitation light source 4 is placed in the light source installation hole 304, the fluorescent excitation light source 4 is turned on, and the reaction tube 5 is irradiated. The fluorescent dye in the reaction tube 5 generates a corresponding fluorescent signal under the excitation of the fluorescent excitation light source 4, and the spectral sensor 6 can collect and record the fluorescent signal in real time, and use it for analysis and interpretation of the detection results. Due to the use of the spectral sensor 6, multi-channel fluorescence detection can be achieved through a detection hole 305 and a spectral sensor 6, replacing multiple emission light filters and multiple photoelectric detection elements, thereby making the overall volume of the fluorescence detector smaller, the production cost lower, and easy to carry.

[0044] Of course, in this embodiment, the excitation light assembly can also include an excitation light filter embedded in the light source mounting hole 304, and the excitation light filter is located between the fluorescent excitation light source 4 and the reaction tube 5. In this embodiment, installing the excitation light filter can achieve better detection effects.

[0045] Of course, in this embodiment, the excitation light filter may not be installed, so as to further save the production cost of the fluorescence detector.

[0046] Furthermore, the spectral sensor 6 can adopt an OSRAM AS7341 spectral sensor, which is low in cost, small in size and very sensitive, thereby being able to significantly reduce the cost and size of the fluorescence detector.

[0047] It is important to know that the AS7341 spectral sensor integrates 8 visible light channels and 1 near-infrared light channel, which can meet the requirements of multi-channel detection. Under the excitation of the fluorescent excitation light source 4, the emitted light can be collected and recorded in real time by the AS7341 spectral sensor and used for analysis and interpretation of the results.

[0048] Of course, in this embodiment, other signal spectral sensors 6 can also be used, such as AS7343 spectral sensor, which can also realize multi-channel detection and reduce the volume of the fluorescence detector. Its purpose does not deviate from the design concept of the utility model, and therefore, it should belong to the protection scope of the utility model.

[0049] Further, such as Figure 1 As shown, its overall structure is simple, and its appearance size is similar to the common Bluetooth headset cabin on the market. The total cost of its optical, mechanical and electronic components is only about 100 yuan, which is low in price and thus low in production cost.

[0050] According to one embodiment of the utility model, Figure 6 As shown, three fluorescent excitation light sources 4 are provided, and three light source mounting holes 304 are opened on the combination plate 3 . The light source mounting holes 304 correspond to the fluorescent excitation light sources 4 one by one.

[0051] Furthermore, in actual use, when the fluorescence detector is used, Figure 1 As shown, the mouth of the reaction tube 5 faces upward, the combined board 3 includes an upper surface, a lower surface and four side surfaces, the placement groove 303 is opened on the upper surface of the combined board 3, the side of the combined board 3 close to the circuit board 2 is recorded as the first surface, and the surface parallel to the first surface is recorded as the second surface, and the other two side surfaces of the combined board 3 are the third surface and the fourth surface respectively, three light source mounting holes 304 are respectively opened on the third surface, the fourth surface and the bottom surface, and are all connected with the placement groove 303, the detection hole 305 is opened on the first surface, and the spectral sensor 6 detects the fluorescence signal through the detection hole 305.

[0052] It should be noted that the reaction tube 5 is a transparent tube made of PP (polypropylene) material. In actual testing, after detecting the fluorescent signal of the nucleic acid detection system in a reaction tube 5, the reaction tube 5 is directly taken out from the placement slot 303 and replaced with the next reaction tube 5 containing another nucleic acid detection system to continue the next test.

[0053] The reaction tube 5 includes a tube body and a tube cover. One side of the tube cover is hinged to the tube body. The tube cover is used to cover the tube body to prevent the reaction tube 5 from leaking nucleic acid amplification products when the cover is opened, thereby causing aerosol contamination.

[0054] Of course, in this embodiment, two fluorescent excitation light sources 4 can be provided, and two light source mounting holes 304 are provided on the combination plate 3, and the light source mounting holes 304 are provided one-to-one with the fluorescent excitation light sources 4. Multi-channel detection can also be achieved, and its purpose does not deviate from the design concept of the utility model, and therefore, it should belong to the protection scope of the utility model.

[0055] It should be noted that by setting several groups of fluorescence excitation light sources 4, several groups of channels can be detected: specifically, a light source mounting hole 304 is set, and the fluorescence excitation light source 4 is installed in the light source mounting hole 304, so that the monkeypox virus can be detected;

[0056] Two light source mounting holes 304 are provided, and the fluorescence excitation light sources 4 are respectively installed in the two light source mounting holes 304, so that the detection of HPV16 and HPV18 viruses can be realized.

[0057] According to one embodiment of the utility model, Figure 4 and Figure 5 As shown, the combined board 3 includes a metal block 301 and a mounting plate 302. A slot is provided at one end of the mounting plate 302 near the first opening, and the metal block 301 is fixedly connected in the slot. Specifically, a first circular slot is provided on the metal block 301, and a first through hole is provided on the mounting plate 302. The first through hole is connected with the first circular slot to form a light source mounting hole 304. The placement slot 303 and the detection hole 305 are both provided on the metal block 301.

[0058] Furthermore, the radius of the first circular groove is smaller than the radius of the first through hole, and the fluorescence excitation light source 4 is embedded in the first through hole. The light emitted by the fluorescence excitation light source 4 is irradiated into the reaction tube 5 along the first through hole through the second through hole, thereby stimulating the fluorescence signal of the fluorescent dye in the reaction to be tested. The spectral sensor 6 collects and records the fluorescence signal through the detection hole 305 and interprets it.

[0059] Furthermore, the material of the metal block 301 is good at heat conduction. Since the fluorescent excitation light source 4 is installed in the first through hole, in order to protect the fluorescent excitation light source 4, the mounting plate 302 can use a heat insulation plate to better protect the fluorescent excitation light source 4 from being damaged due to excessive temperature.

[0060] It should be noted that there is no other component blocking the metal block 301 from the side close to the circuit board 2 to the circuit board 2 , and the spectrum sensor 6 can directly collect the fluorescent signal emitted by the fluorescent dye in the reaction tube 5 through the detection hole 305 .

[0061] Of course, in this embodiment, the combined plate 3 is a heat-conducting metal plate as a whole, and is provided with a placement groove 303, a light source mounting hole 304 and a detection hole 305, which can also achieve the purpose of placing the reaction tube 5 and the fluorescent excitation light source 4. Its purpose does not deviate from the design concept of the present utility model, and therefore, should belong to the protection scope of the present utility model.

[0062] According to one embodiment of the utility model, Figure 4 and Figure 5 As shown, a temperature control module is installed in the shell 1 , and the portable multi-channel fluorescent nucleic acid detector also includes a lysis tube for sample processing. The temperature control module is used to detect and control the lysis temperature of the lysis tube and the reaction temperature of the reaction tube 5 .

[0063] Specifically, the temperature control module includes a temperature sensor 13, a heating plate 9, a fan 7 and a control unit, and the temperature sensor 13, the heating plate 9 and the fan 7 are all electrically connected to the control unit;

[0064] One side of the card slot is connected to a clearance groove, the heating plate 9 is arranged in the clearance groove, the temperature sensor 13 is installed on the metal block 301, and the fan 7 is installed on the side of the combination board 3 away from the circuit board 2;

[0065] The control unit is integrated on a single chip microcomputer.

[0066] When the pathogen in the lysis tube is lysed to release nucleic acid, the control unit controls the heating plate 9 to start until the temperature sensor 13 detects that the lysis temperature in the lysis tube is equal to the first temperature threshold, the temperature sensor 13 transmits the temperature signal to the control unit, and the control unit controls the heating plate 9 to maintain the temperature at the first temperature threshold for a period of time and then stop;

[0067] When the nucleic acid detection reagent reacts in the reaction tube 5, the control unit controls the fan 7 to turn on until the temperature sensor 13 detects that the reaction temperature in the reaction tube 5 is equal to the preset second temperature threshold, and then stops. After the control unit controls the temperature of the heating plate 9 to maintain at the second temperature threshold for a period of time, the control unit controls the heating plate 9 to stop.

[0068] Furthermore, in order to make the temperature of the reaction tube 5 during cracking and reaction more accurate, the single chip microcomputer can also control the power of the fan 7 and the heating plate 9 at the same time.

[0069] The heating plate 9 can use a ceramic heating plate to heat the metal block 301, thereby quickly heating the lysis tube to a set first temperature threshold, i.e., the lysis temperature (80°C). After lysis, part of the lysis product in the lysis tube is added to the reaction tube 5 containing the nucleic acid detection system, and the detection reagent in the reaction tube 5 is maintained at a set second temperature threshold, i.e., the reaction temperature (37°C).

[0070] Of course, in this embodiment, the heating plate 9 can also be a resistor plate, and can also achieve heating of the metal block 301. Its purpose does not deviate from the design concept of the utility model, and therefore, it should belong to the protection scope of the utility model.

[0071] According to one embodiment of the utility model, Figure 1 As shown, a first button 11 for controlling cracking and a second button 12 for controlling reaction temperature are provided on the housing 1, and both the first button 11 and the second button 12 are electrically connected to the single chip computer.

[0072] Further, in actual use, the temperature sensor 13 can be selected from a PT1000 temperature sensor, the single-chip microcomputer can be selected from an ESP32 single-chip microcomputer, and the ceramic heating plate and the PT1000 temperature sensor are controlled by the ESP32 single-chip microcomputer through a proportional integral differential algorithm. The lysis tube containing the pathogen is placed in the placement slot 303, and the ceramic heating plate is heated to the lysis temperature by pressing the first button 11, so that the pathogen in the lysis tube is lysed and released under the action of the lysis solution. The nucleic acid target. After the lysis is completed, the lysis tube is taken out, and the lysis product in the lysis tube is transferred to the reaction tube 5 containing the nucleic acid detection system, and the reaction tube 5 is placed in the placement slot 303, and the second button 12 is pressed. The temperature of the metal block 301 is maintained at the reaction temperature (37°C), thereby maintaining the temperature in the reaction tube 5 at 37°C. It should be noted here that in this embodiment, the sample lysis process and the nucleic acid detection reaction are completed in the placement slot 303, which saves more space, thereby reducing the volume of the fluorescence detector and making it easy to carry.

[0073] The first button 11 and the second button 12 are provided to make the operation process more convenient, and the provision of the first button 11 and the second button 12 can also play a fool-proof effect.

[0074] According to one embodiment of the utility model, Figure 3 As shown, the housing 1 is provided with a plurality of heat dissipation holes 101 to facilitate heat dissipation by the fan 7 .

[0075] By using the fan 7, after the fluorescent dye is cracked, the internal heat can be quickly discharged from the multiple heat dissipation holes 101, so that the temperature is reduced to the reaction temperature (37° C.), the heat dissipation efficiency is accelerated, and the detection time is shortened.

[0076] According to one embodiment of the utility model, Figure 1 As shown, a fixing plate 103 is installed at the first opening, a second through hole is provided on the fixing plate 103 , and one end of the reaction tube 5 passes through the second through hole and is embedded in the placement groove 303 .

[0077] Specifically, installing the fixing plate 103 can make the reaction tube 5 more stably placed in the placement groove 303. In order to enhance the stability of the reaction tube 5, a retaining component can be provided at the second through hole. Preferably, an annular rubber ring is fixed in the second through hole. When the reaction tube 5 is inserted into the annular rubber ring, the annular rubber ring is squeezed, so that the reaction tube 5 can be clamped more tightly and has better stability.

[0078] Of course, in this embodiment, two elastic telescopic parts (including a telescopic rod and a spring installed in the telescopic rod, and the telescopic rod is used to limit the telescopic direction of the spring) can be arranged above the second through hole, and an arc plate can be installed at one end of the elastic telescopic part close to the reaction tube 5. The two arc plates cooperate to clamp and align the reaction tube 5 to fix it in place, so that it is not easy to slip off.

[0079] According to one embodiment of the utility model, Figure 1 As shown, two indicator lights 10 are provided on the housing 1, and both indicator lights 10 are electrically connected to the single chip computer. Each indicator light 10 has two colors. The two indicator lights 10 have two functions, one indicator light 10 has two colors, red and blue, representing the pyrolysis temperature and the reaction temperature, respectively, and the other indicator light 10 has two colors, green and red, representing the results of the nucleic acid test (i.e., the two colors represent negative and positive, respectively).

[0080] For user-friendly considerations, the instrument has two different diagnostic result interpretation modes: stand-alone mode and wireless control mode. Users can choose according to their own needs. In stand-alone mode, the color status of indicator light 10 can be used for qualitative interpretation by naked eyes. The nucleic acid detector also includes a wireless communication module that can be connected to a mobile phone via Bluetooth or WIFI. The collected fluorescence signal can be displayed in real time on the smartphone application interface, and the result is finally determined by comparing the fluorescence signal intensity with the set threshold.

[0081] Specifically, a power interface 8 adapted to the power supply is provided in the installation cavity, the power interface 8 is electrically connected to the single-chip computer, a bottom plate is installed at one end of the installation cavity away from the first opening, the bottom plate is fixedly connected to the housing 1, a support plate is fixed on the bottom plate, the power interface 8 is connected to the support plate, and the support plate plays a supporting role for the power interface 8. An external power supply is connected to the power interface 8 through a data cable to power the fluorescence detector. In view of the advantages of the fluorescence detector, such as miniaturization, low cost, multi-function, and easy operation, it has broad application prospects in primary medical institutions, families, and even individuals in high-sensitivity rapid nucleic acid testing.

[0082] Of course, in this embodiment, a battery can be installed in the housing 1, and the battery is electrically connected to the circuit board 2, so as to power the fluorescence detector. The purpose does not deviate from the design concept of the utility model, and therefore, it should belong to the protection scope of the utility model.

[0083] Furthermore, in this embodiment, a magnetic attraction component is provided between the shell cover 102 and the fixing plate 103 to facilitate opening and closing of the shell cover 102. The magnetic attraction component is a prior art and will not be described in detail herein.

[0084] Of course, in this embodiment, a snap-fit ​​structure can be provided between the shell cover 102 and the fixed plate 103 so that the shell cover 102 is snap-fitted with the fixed plate 103, and the shell cover 102 can also be closed to protect the reaction tube 5. It should be noted that when the shell cover 102 is closed, the tube cover of the reaction tube 5 abuts against the shell cover 102, and the shell cover 102 abuts against the tube cover to prevent the reaction tube 5 from opening the cover to leak the nucleic acid amplification product and cause aerosol contamination.

[0085] In the description of the present invention, it should be noted that the terms "upper" and "lower" and the like indicate positions or positional relationships based on the positions or positional relationships shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific position, be constructed and operated in a specific position, and therefore cannot be understood as limiting the present invention. In addition, the terms "first" and "second" are only used for descriptive purposes and cannot be understood as indicating or implying relative importance.

[0086] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installation", "connection" and "connection" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be an indirect connection through an intermediate medium, or it can be a connection between the insides of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances. In addition, in the description of the present invention, unless otherwise specified, "plurality" means two or more.

[0087] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A portable multi-channel fluorescent nucleic acid detector, characterized in that: include: A housing (1), a circuit board (2), a reaction tube (5) and a fluorescence detection module; The housing (1) is provided with an installation cavity, one end of which is connected to a first opening; The circuit board (2) is arranged in the installation cavity, a single-chip microcomputer is installed on the circuit board (2), the fluorescence detection module comprises a spectrum sensor (6) installed on the circuit board (2), and the spectrum sensor (6) is signal-connected to the single-chip microcomputer; The fluorescence detection module further comprises an excitation light component, the reaction tube (5) is installed in the excitation light component, the excitation light component excites the fluorescence signal of the fluorescent dye in the reaction tube (5), and the spectrum sensor (6) is used to collect the fluorescence signal.

2. The portable multi-channel fluorescent nucleic acid detector according to claim 1, characterized in that: The excitation light assembly comprises a combination plate (3) and a fluorescent excitation light source (4); a placement groove (303) for placing the reaction tube (5) is provided at one end of the combination plate (3) close to the first opening; a light source mounting hole (304) for placing the fluorescent excitation light source (4) is provided on the combination plate (3); the light source mounting hole (304) is communicated with the placement groove (303); The combined plate (3) is also provided with a detection hole (305), the spectrum sensor (6) is arranged at one end of the detection hole (305), and the other end of the detection hole (305) is connected to the placement groove (303).

3. The portable multi-channel fluorescent nucleic acid detector according to claim 2, characterized in that: A plurality of the fluorescent excitation light sources (4) are provided, and a plurality of the light source mounting holes (304) are provided on the combined plate (3), and the plurality of the light source mounting holes (304) correspond one-to-one to the plurality of the fluorescent excitation light sources (4).

4. The portable multi-channel fluorescent nucleic acid detector according to claim 2, characterized in that: An excitation light filter is embedded in the light source installation hole (304), and the excitation light filter is located between the fluorescent excitation light source (4) and the reaction tube (5).

5. The portable multi-channel fluorescent nucleic acid detector according to claim 2, characterized in that: The combined plate (3) comprises a metal block (301) and a mounting plate (302); a slot is provided at one end of the mounting plate (302) close to the first opening, and the metal block (301) is fixedly connected in the slot.

6. The portable multi-channel fluorescent nucleic acid detector according to claim 5, characterized in that: The metal block (301) is provided with a first circular groove, the mounting plate (302) is provided with a first through hole, and the first through hole is connected to the first circular groove to form the light source mounting hole (304); The placement groove (303) and the detection hole (305) are both provided on the metal block (301).

7. The portable multi-channel fluorescent nucleic acid detector according to claim 5, characterized in that: A temperature control module is installed in the housing (1); the portable multi-channel fluorescent nucleic acid detector also includes a pyrolysis tube for sample processing; the temperature control module is used to detect and control the pyrolysis temperature of the pyrolysis tube and the reaction temperature of the reaction tube (5).

8. The portable multi-channel fluorescent nucleic acid detector according to claim 7, characterized in that: The temperature control module comprises a temperature sensor (13), a heating plate (9), a fan (7) and a control unit, wherein the temperature sensor (13), the heating plate (9) and the fan (7) are all electrically connected to the control unit; One side of the card slot is connected to a clearance groove, the heating plate (9) is arranged in the clearance groove, the temperature sensor (13) is installed on the metal block (301), and the fan (7) is installed on a side of the combined board (3) away from the circuit board (2).

9. The portable multi-channel fluorescent nucleic acid detector according to claim 8, characterized in that: When the pathogen in the lysis tube is lysed to release nucleic acid, the control unit controls the heating plate (9) to start until the temperature sensor (13) detects that the lysis temperature in the lysis tube is equal to a first temperature threshold, the temperature sensor (13) transmits a temperature signal to the control unit, and the control unit controls the heating plate (9) to maintain the temperature at the first temperature threshold for a period of time and then stops; When the nucleic acid detection reagent reacts in the reaction tube (5), the control unit controls the fan (7) to turn on until the temperature sensor (13) detects that the reaction temperature in the reaction tube (5) is equal to a preset second temperature threshold value, and the temperature sensor (13) transmits a temperature signal to the control unit, and the control unit controls the fan (7) to stop and controls the temperature of the heating plate (9) to maintain at the second temperature threshold value for a period of time through the control unit, and then the control unit controls the heating plate (9) to stop.

10. The portable multi-channel fluorescent nucleic acid detector according to claim 7, characterized in that: The housing (1) is provided with a first button (11) and a second button (12), and the first button (11) and the second button (12) are both electrically connected to the single-chip computer; Two indicator lights (10) are arranged on the housing (1), and both of the two indicator lights (10) are electrically connected to the single chip computer.

Images