Stirring type illumination bioreactor

Abstract

The utility model provides a stirring type illumination bioreactor which comprises a reaction tank, a cover body used for covering the reaction tank, a stirring assembly used for being inserted into the reaction tank to stir reaction liquid, and an illumination assembly used for being inserted into the reaction tank to irradiate the reaction liquid, the illumination assembly comprises an isolation pipe accommodated in the reaction tank and a lamp strip accommodated in the isolation pipe, and the isolation pipe is used for isolating the reaction liquid from the lamp strip. According to the stirring type illumination bioreactor disclosed by the utility model, the illumination assembly inserted into the reaction tank is arranged, so that higher light intensity can be provided for a culture system, the structure is simple, and the light utilization rate is high; by arranging the isolation pipe, the reaction liquid is isolated from the lamp strip, the lamp strip is prevented from polluting the reaction liquid, and heat can also be prevented from being transferred to the reaction liquid.

Description

Technical Field

[0001] The utility model relates to a stirring type light irradiation bioreactor. Background Art

[0002] Microalgae usually refers to the general term for microorganisms that contain chlorophyll a and can perform photosynthesis, and are a type of protists. Microalgae that are cultivated or produced in large quantities using biotechnology belong to four algae phyla: Cyanobacteria, Chlorophyta, Chrysophyta, and Rhodophyta. Chlorella (Chlorella; pyrenoidosa) is a ubiquitous single-cell green algae of the genus Chlorella in the Chlorophyta. It is a spherical single-cell freshwater algae and an efficient photosynthetic plant. It grows and reproduces by photosynthesis and autotrophy. It is widely distributed in freshwater areas, has strong adaptability, good survival ability, and reproduces quickly. The protein nucleus in Chlorella contains rich protein, vitamins, minerals, dietary fiber, nucleic acids, and chlorophyll, and has the functions of lowering lipids, lowering blood pressure, lowering blood sugar, and preventing and improving atherosclerosis. It has high nutritional value and medical value.

[0003] Microalgae culture inevitably requires the use of reactors. The reactor used for Chlorella culture is usually called a photobioreactor. Photobioreactors can be divided into open photobioreactors and closed photobioreactors. The open photobioreactor has a simple structure, low cost and easy operation, but it is greatly affected by the external environment and cannot achieve precise control of physical quantities. Its production is restricted by region and season. The closed photobioreactor is not restricted by region and season. It can artificially construct an environment suitable for the growth of microalgae, and accurately regulate multiple parameters affecting microalgae, which is conducive to relevant scientific research experiments on microalgae. Temperature, light conditions, pH value, dissolved oxygen, feeding method, OD concentration all affect the growth rate and product accumulation of microalgae. How to accurately control the key factors affecting the growth of microalgae and improve the growth rate of microalgae has become the key to the current research on microalgae culture devices.

[0004] A stirred photobioreactor is a common photobioreactor. It has good oxygen supply and mixing effects, but its complex external light source structure and low light intensity utilization limit its large-scale application development.

[0005] In view of this, it is necessary to improve the existing stirred photobioreactor to solve the above problems. Summary of the invention

[0006] The utility model aims to provide a stirring type light irradiation bioreactor to solve the problems of complex structure of external light source and low light intensity utilization rate of the existing stirring type light irradiation bioreactor.

[0007] To achieve the above-mentioned purpose, the utility model provides a stirring type light-irradiation bioreactor, which comprises a reaction tank, a cover for covering the reaction tank, a stirring assembly for inserting the reaction tank to stir the reaction liquid, and an illumination assembly for inserting the reaction tank to irradiate the reaction liquid. The illumination assembly comprises an isolation tube accommodated in the reaction tank and a light strip accommodated in the isolation tube, the isolation tube is used to isolate the reaction liquid and the light strip, and the stirring assembly comprises a stirring shaft inserted into the reaction tank, a stirring motor for driving the stirring shaft to rotate, and a defoaming paddle and a stirring paddle arranged on the stirring shaft.

[0008] As a further improvement of the utility model, the isolation tube is made of transparent material.

[0009] As a further improvement of the utility model, the upper end of the isolation tube is fixed on the cover body and sealed with the cover body.

[0010] As a further improvement of the present invention, the lighting assembly also includes a bracket pole inserted into the isolation tube to support the light strip, and a heat dissipation module, the bracket pole passes through the cover body and protrudes above the cover body, the heat dissipation module is arranged on the bracket pole and is located above the cover body, and the bracket pole is made of heat-conductive material.

[0011] As a further improvement of the present invention, the light strip is a multi-color light strip with adjustable brightness.

[0012] As a further improvement of the present invention, the stirred light-irradiation bioreactor further includes a control component, and the control component is electrically connected to the illumination component.

[0013] As a further improvement of the utility model, the cover body is provided with one or more of a head cover plate, a puncture port, a sampling interface, a feeding / harvesting port, a temperature blind bag, an air distributor, a baffle module, a pH / DO electrode port, an exhaust gas outlet module, an air inlet, an all-in-one feeding interface, and a spare port.

[0014] As a further improvement of the present invention, the stirred photo-bioreactor further includes a tank support for supporting the reaction tank.

[0015] As a further improvement of the utility model, a fixing bracket for supporting the isolation tube is provided on the tank bracket.

[0016] The beneficial effects of the utility model are as follows: the stirring light-irradiation bioreactor of the utility model can provide a higher light intensity for the culture system by arranging a lighting component inserted into the reaction tank, and has a simple structure and high light utilization rate; and by arranging an isolation tube, the reaction liquid is isolated from the light strip to prevent the light strip from contaminating the reaction liquid and also to prevent heat from being transferred to the reaction liquid. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a structural schematic diagram of a stirring photobioreactor of the utility model;

[0018] Figure 2 It is a structural schematic diagram of a reaction tank and a tank support of a stirring light-irradiation bioreactor of the utility model;

[0019] Figure 3 It is a structural schematic diagram of the cover body of the stirring type photo-irradiation bioreactor of the utility model;

[0020] Figure 4 It is a structural schematic diagram of the illumination component of the stirring type illumination bioreactor of the utility model;

[0021] Figure 5 It is a structural schematic diagram of the illumination component of the stirring type illumination bioreactor of the utility model without the isolation tube;

[0022] Figure 6 It is a structural schematic diagram of the isolation tube of the stirring photobioreactor of the utility model;

[0023] Figure 7 It is a partial structural schematic diagram of the illumination assembly and the cover body of the stirring type illumination bioreactor of the utility model;

[0024] Figure 8 It is a structural schematic diagram of a stirring assembly of a stirring photobioreactor of the utility model;

[0025] Fig. 9 The utility model is a graph showing the growth trend of Chlorella when the stirring light bioreactor is used for culturing Chlorella. Implementation

[0026] The technical solution of the utility model will be described clearly and completely below in conjunction with the accompanying drawings. Obviously, the described embodiments are part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments of the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0027] In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicating the orientation or positional relationship, are based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present invention. In addition, the terms "first", "second", and "third" are used for descriptive purposes only, and cannot be understood as indicating or implying relative importance.

[0028] In the description of the present utility model, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "connected" and "connected" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal connection of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present utility model can be understood according to specific circumstances. In addition, the technical features involved in the different embodiments of the present utility model described below can be combined with each other as long as they do not conflict with each other.

[0029] like Figures 1 to 8 As shown, the stirring photobioreactor 100 of the utility model includes a reaction tank 1, a cover 2 for covering the reaction tank 1, a stirring component 3 for inserting into the reaction tank 1 to stir the reaction liquid, an illumination component 4 for inserting into the reaction tank 1 to irradiate the reaction liquid, a tank bracket 5 for supporting the reaction tank 1, and a control component 6.

[0030] The reaction tank 1 is used to contain the reaction liquid. In this embodiment, the stirring light bioreactor 100 is mainly used for algae cultivation.

[0031] like Figure 3 As shown, the cover body 2 is provided with one or more of a head cover plate 21, a puncture port 22, a sampling port 23, a feeding / harvesting port 24, a temperature blind bag 25, an air distributor 26, a baffle module 27, a pH / DO electrode port 28, an exhaust gas outlet module 201, an air inlet 202, an all-in-one feeding port 203, and a spare port 204. Some of the structures need to be inserted from the cover body 2 into the reaction tank 1.

[0032] like Figures 4 to 7 As shown, the lighting assembly 4 includes an isolation tube 41 housed in the reaction tank 1 , a light strip 42 housed in the isolation tube 41 , a support rod 43 inserted into the isolation tube 41 to support the light strip 42 , and a heat dissipation module 44 .

[0033] Inserting the light strip 42 and the isolation tube 41 into the reaction tank 1 can provide a higher light intensity for the culture system, and the light utilization rate is high.

[0034] The isolation tube 41 is used to isolate the reaction liquid and the light strip 42. In this embodiment, the isolation tube 41 is made of transparent material, so that the optical fiber of the light strip 42 can pass through the isolation tube 41 to irradiate the reaction liquid, preventing the light strip 42 from contaminating the reaction liquid and ensuring the sterility of the reaction liquid. At the same time, the light strip 42 is arranged in the middle of the isolation tube 41 and is spaced from the tube wall of the isolation tube 41. The isolation tube 41 can also play a certain role in heat insulation, preventing the heat of the light strip 42 from affecting the temperature of the reaction.

[0035] The upper end of the isolation tube 41 is fixed on the cover 2 and sealed with the cover 2. This prevents the reaction liquid from entering the isolation tube 41 from the cover 2. The tank support 5 is provided with a fixing support 51 for supporting the isolation tube 41.

[0036] The support rod 43 passes through the cover body 2 and protrudes above the cover body 2 . The heat dissipation module 44 is disposed on the support rod 43 and is located above the cover body 2 .

[0037] The support rod 43 is made of heat-conducting material, so that the heat emitted by the light strip 42 can be transferred to the support rod 43 .

[0038] The heat dissipation module 44 is located above the cover 2 and connected to the support rod 43 to discharge the heat of the support rod 43, improve the heat dissipation effect, and prevent the temperature of the light strip 42 from affecting the temperature of the reaction liquid. In this embodiment, the heat dissipation module 44 is a heat dissipation fan.

[0039] The light strip 42 is a multi-color light source with adjustable brightness. The light strip 42 uses RGB three-color adjustable light sources, and the brightness of each light source is divided into 256 levels (0-255). The designed light intensity can be automatically adjusted according to different strain cultivation requirements (the average maximum light intensity can reach more than 50,000 Lux), so it can meet the cultivation of different project strains with different light intensity requirements.

[0040] The control component 6 is electrically connected to the illumination component 4. The control unit integrates a step-by-step design of light levels, which can adjust the light parameters in a gradient manner and perform a cyclic control of the light-dark ratio, thus meeting the process growth requirements of strains of different projects.

[0041] like Figure 8 As shown, the stirring assembly 3 includes a stirring shaft 31 inserted into the reaction tank 1 , a stirring motor 32 for driving the stirring shaft 31 to rotate, and a defoaming paddle 33 and a stirring paddle 34 arranged on the stirring shaft 31 .

[0042] The application of the stirring photobioreactor 100 of the utility model in the cultivation of Chlorella is as follows:

[0043] a. Preparation of algae species: FACHB-5.

[0044] b. Preparation of shake flask seeds: The shake flask medium is BG11 medium, the liquid volume is 200ml / 500ml, sterilized at 121℃ for 20min, and the inoculation amount is 5% after cooling. Culture at 25℃, 100rpm, and light-dark ratio of 20h:4h for 10 days.

[0045] The BG11 components include 1.5g / L sodium nitrate, 0.04g / L dipotassium hydrogen phosphate, 0.075g / L magnesium sulfate heptahydrate, 0.036g / L calcium chloride dihydrate, 0.006g / L citric acid, 0.006g / L ferric ammonium citrate, 0.001g / L EDTA-disodium, 0.02g / L sodium carbonate, and 1ml / L trace element mother solution (the trace element mother solution is 2.86g / L boric acid, 1.86g / L manganese chloride tetrahydrate, 0.22g / L zinc sulfate heptahydrate, 0.39g / L sodium molybdate dihydrate, 0.08g / L copper sulfate pentahydrate, and 0.05g / L cobalt nitrate hexahydrate).

[0046] C. Preparation for tank loading: A stirred photobioreactor 100 was selected, wherein the volume of the reaction tank 1 was 5 L, the liquid volume was 3 L, the initial fermentation medium was BG11, the feed medium was BG11 triple concentrate, the inoculation amount was 5% of the shake flask seed liquid (OD6700.685), and the process parameters were set as shown in Table 1:

[0047] Table 1, Fermentation culture parameter settings:

[0048] D. Shake flask control preparation: The same seed preparation parameters were used to control the conditions. The shake flask control was inoculated at the same time as the photobioreactor and cultured synchronously for 13 days as the photobioreactor.

[0049] Fermentation result analysis

[0050] like Fig. 9As shown, the experimental culture cycle of this embodiment is 13 days, in which 50 ml of BG11 triple concentrate was added once on the 7th, 9th and 11th days respectively, and the best result was obtained on the 12th day. The result data is shown in the growth trend curve, in which the OD670 value is 1.676, the algae density is 6.45×107 / ml, and the cell dry weight is 2.4g / L. Compared with the experimental yield of the shake flask in the same period, the OD670 value of this experiment increased by 67.6% (the OD670 value of the shake flask was 0.946), the algae density value increased by 168% (the algae density value of the shake flask was 2.4×107 / ml), and the cell dry weight increased by 152% (the cell dry weight of the shake flask was 0.95g / L).

[0051] The stirring light-irradiation bioreactor 100 of the utility model can provide a higher light intensity for the culture system by arranging a lighting component 4 inserted into the reaction tank 1, and has a simple structure and high light utilization rate; by arranging an isolation tube 41, the reaction liquid and the light strip 42 are isolated to prevent the light strip 42 from contaminating the reaction liquid and also to prevent heat from being transferred to the reaction liquid.

[0052] The technical features of the above-described embodiments may be arbitrarily combined. To make the description concise, not all possible combinations of the technical features in the above-described embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0053] The above-mentioned embodiments only express several implementation methods of the utility model, and the description is relatively specific and detailed, but it cannot be understood as limiting the scope of the utility model patent. It should be pointed out that for ordinary technicians in this field, several modifications and improvements can be made without departing from the concept of the utility model, which all belong to the protection scope of the utility model. Therefore, the protection scope of the utility model patent shall be based on the attached claims.

Claims

1. A stirred photobioreactor, characterized in that: The stirring light-irradiation bioreactor comprises a reaction tank, a cover for covering the reaction tank, a stirring assembly for inserting into the reaction tank to stir the reaction liquid, and an illumination assembly for inserting into the reaction tank to irradiate the reaction liquid. The illumination assembly comprises an isolation tube accommodated in the reaction tank and a light strip accommodated in the isolation tube, the isolation tube is used to isolate the reaction liquid and the light strip. The stirring assembly comprises a stirring shaft inserted into the reaction tank, a stirring motor for driving the stirring shaft to rotate, and a defoaming paddle and a stirring paddle arranged on the stirring shaft.

2. The stirred photobioreactor according to claim 1, characterized in that: The isolation tube is made of transparent material.

3. The stirred photobioreactor according to claim 1, characterized in that: The upper end of the isolation tube is fixed on the cover body and sealed with the cover body.

4. The stirred photobioreactor according to claim 1, characterized in that: The lighting assembly also includes a support pole inserted into the isolation tube to support the light strip, and a heat dissipation module. The support pole passes through the cover body and protrudes above the cover body. The heat dissipation module is arranged on the support pole and is located above the cover body. The support pole is made of heat-conducting material.

5. The stirred photobioreactor according to claim 1, characterized in that: The light strip is a multi-color light source light strip with adjustable brightness.

6. The stirred photobioreactor according to any one of claims 1 to 5, characterized in that: The stirred-type photo-bioreactor further comprises a control component, and the control component is electrically connected to the photo-bioreactor.

7. The stirred photobioreactor according to claim 1, characterized in that: The cover body is provided with one or more of a head cover plate, a puncture port, a sampling interface, a feeding / harvesting port, a temperature blind bag, an air distributor, a baffle module, a pH / DO electrode port, an exhaust gas outlet module, an air inlet, an all-in-one feeding interface, and a spare port.

8. The stirred photobioreactor according to claim 1, characterized in that: The stirred photo-bioreactor further comprises a tank support for supporting the reaction tank.

9. The stirred photobioreactor according to claim 8, characterized in that: The tank support is provided with a fixing support for supporting the isolation tube.

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