Separation and extraction equipment for plant exosomes

Abstract

The utility model relates to separation and extraction equipment for plant exosomes. The separation and extraction equipment comprises a liquid storage container, a deep filter, a pre-filter, a sterilization filter and a collector, the liquid storage container is used for storing plant juice; the deep filter is communicated with the liquid storage container through a first pumping device; the pre-filter is communicated with the deep filter through a second pumping device; the sterilization filter is communicated with the pre-filter through a third pumping device; the collector is communicated with the sterilization filter so as to collect the plant exosomes which are separated and extracted from the plant juice step by step through the deep filter, the pre-filter and the sterilization filter which are connected in series. The separation and extraction equipment is simple in structure, convenient to install and stable in work, parts do not need to be repeatedly disassembled and assembled, and the separated and extracted plant exosomes can be conveniently collected and reliably stored and transported.

Description

Technical Field

[0001] The utility model relates to the technical field of exosome extraction, and in particular to a separation and extraction device for plant exosomes. Background Art

[0002] Exosomes can be extracted from animals or plants. They are nanoparticles with a phospholipid bilayer secreted by cells. They are usually 30-200nm in diameter and contain rich proteins and nucleic acids. Exosomes can transfer active substances such as proteins, mRNA, and microRNA between cells and participate in many important physiological and pathological processes. Their unique role has attracted more and more attention in the industry.

[0003] Animal-derived exosomes are mainly obtained from milk samples, various cell culture supernatants, exudates from tumor tissues, etc. However, the material sources for obtaining exosomes from sources such as cell culture supernatants are limited, there is potential immune stimulation, the cost is high, the content of the obtained exosomes is low, and the cost after purification is higher, which restricts the large-scale industrial production of exosomes.

[0004] Various plants are also important sources of exosomes, and the extraction rate of plant-derived exosome-like nanoparticles (PEN) is much higher than that of mammalian cell cultures. Plant exosomes are similar to mammalian exosomes in structure and function. Studies have shown that exosomes extracted from plants such as ginger, ginseng, wheat, rhodiola rosea, and licorice not only have similar anti-fibrosis, antiviral, and anti-tumor functions as animal exosomes, but also make up for their partial immunogenicity and limited sources.

[0005] Common extraction methods for plant exosomes in the prior art include ultracentrifugation and sucrose density gradient centrifugation, etc. However, these methods require repeated centrifugation, and have the disadvantages of cumbersome separation operation, time-consuming process, complex equipment and high cost, and low exosome recovery rate. Utility Model Content

[0006] The purpose of the present invention is to solve at least one of the above problems and / or other defects in the prior art.

[0007] To achieve the above-mentioned purpose, according to one aspect of the utility model, a separation and extraction device for plant exosomes is provided, the separation and extraction device comprising a liquid storage container, a depth filter, a pre-filter, a sterilizing filter and a collector. The liquid storage container is used to store plant juice; the depth filter is connected to the liquid storage container via a first pumping device; the pre-filter is connected to the depth filter via a second pumping device; the sterilizing filter is connected to the pre-filter via a third pumping device; the collector is connected to the sterilizing filter to collect the plant exosomes separated and extracted from the plant juice step by step through the depth filter, the pre-filter and the sterilizing filter connected in series.

[0008] According to one embodiment of the utility model, the liquid storage container includes a container body erected on a supporting surface, the bottom of the container body is a funnel-shaped structure that tapers downward, and a liquid outlet interface of the liquid storage container is provided at the bottom end of the funnel-shaped structure, and the liquid outlet interface of the liquid storage container is connected to the deep filter via a first pipeline provided with the first pumping device.

[0009] According to one embodiment of the utility model, the deep filter comprises a vertically arranged deep filter housing, and the deep filter housing is provided with a deep filter liquid inlet interface and a deep filter liquid outlet interface at the top and bottom ends respectively, the deep filter liquid inlet interface is connected to the liquid outlet interface of the liquid storage container via the first pipeline, and the deep filter liquid outlet interface is connected to the pre-filter via a second pipeline provided with the second pumping device.

[0010] According to one embodiment of the utility model, the deep filter also includes a plurality of layers of filter membranes stacked in the deep filter housing, each layer of the filter membranes having a pore size of 2 to 30 microns, and including an upper filter plate and a lower filter plate stacked on each other, the pore size of the upper filter plate being larger than the pore size of the lower filter plate.

[0011] According to one embodiment of the utility model, the pre-filter includes a vertically arranged pre-filter housing, and the pre-filter housing is respectively provided with a pre-filter liquid inlet interface and a pre-filter liquid outlet interface at the top and bottom ends thereof, the pre-filter liquid inlet interface is connected to the deep filter liquid outlet interface via the second pipeline, and the pre-filter liquid outlet interface is connected to the sterilizing filter via a third pipeline provided with the third pumping device.

[0012] According to one embodiment of the present invention, the pre-filter further comprises a pre-filter membrane disposed in the pre-filter housing in a folded or stacked manner, and the pore size of the pre-filter membrane is 0.4 to 0.8 microns.

[0013] According to one embodiment of the utility model, the sterilizing filter includes a vertically arranged sterilizing filter shell, and the sterilizing filter shell is respectively provided with a sterilizing filter liquid inlet interface and a sterilizing filter liquid outlet interface at the top and bottom ends thereof, the sterilizing filter liquid inlet interface is connected to the pre-filter liquid outlet interface via the third pipeline, and the sterilizing filter liquid outlet interface is connected to the collector via the fourth pipeline.

[0014] According to one embodiment of the utility model, the sterilizing filter further comprises a sterilizing filter membrane arranged in the sterilizing filter housing in a folded or stacked manner, and the pore size of the sterilizing filter membrane is 0.1 to 0.3 microns.

[0015] According to one embodiment of the utility model, the liquid outlet interface of the liquid storage container, the liquid inlet interface of the deep filter, the liquid outlet interface of the deep filter, the liquid inlet interface of the pre-filter and the liquid outlet interface of the pre-filter are flange interfaces of the same specifications, and the liquid inlet interface of the sterilizing filter and the liquid outlet interface of the sterilizing filter are barbed interfaces of the same specifications.

[0016] According to one embodiment of the utility model, the collector includes a liquid storage bag made of EVA (ethylene-vinyl acetate copolymer), and the liquid storage bag is provided with a sampling port and a collector liquid inlet interface connected to the liquid outlet interface of the sterilizing filter via the fourth pipeline with a throttling clamp.

[0017] The separation and extraction device for plant exosomes of the utility model allows plant juice to continuously pass through a plurality of filters with appropriate filter apertures connected in series according to the size of the plant exosomes to be separated and extracted, thereby separating and extracting the desired plant exosomes from the plant juice step by step or layer by layer. The separation and extraction of plant exosomes is continuously carried out through this step-by-step filtering device, and the process is simple and efficient. While ensuring the extraction amount and purity of plant exosomes, the separation and extraction time can be greatly shortened, the yield can be significantly increased, the product can be guaranteed to be sterile, and the activity of exosomes is not affected. The separation and extraction device of the utility model has a simple structure, is easy to install, and works stably. There is no need to repeatedly disassemble and assemble parts, and the separated and extracted plant exosomes can be conveniently collected and reliably stored and transported. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] The features and advantages of the present invention will be clearly understood through the detailed description provided below with reference to the accompanying drawings. It should be understood that the following drawings are merely schematic and not necessarily drawn to scale, and thus cannot be regarded as limiting the present invention, wherein:

[0019] Figure 1 An overall schematic diagram of a device for separating and extracting plant exosomes according to an embodiment of the utility model is shown.

[0020] Figure 2 Show Figure 1 A schematic diagram of a liquid storage container in a separation and extraction device is shown.

[0021] Figure 3 Show Figure 1 Schematic diagram of the depth filter in the separation and extraction equipment shown.

[0022] Figure 4 Show Figure 1 Schematic diagram of the pre-filter in the separation and extraction equipment shown.

[0023] Figure 5 Show Figure 1 Schematic diagram of the sterilizing filter in the separation and extraction equipment shown.

[0024] Figure 6 Show Figure 1 Schematic diagram of the collector in the separation and extraction equipment shown.

[0025] Description of reference numerals:

[0026] 10. Liquid storage container; 11. Container body; 111. Opening; 112. Liquid outlet interface of liquid storage container; 12. Container cover; 121. Air outlet; 20. Deep filter; 21. Deep filter housing; 211. Liquid inlet interface of deep filter; 212. Liquid outlet interface of deep filter; 213. Air vent of deep filter; 214. Drain port of deep filter; 30. First pumping device; 31. First pipeline; 40. Pre-filter; 41. Pre-filter housing; 411. Liquid inlet interface of pre-filter; 412. Pre-filter liquid outlet interface; 413, pre-filter air vent; 414, pre-filter drain outlet; 50, second pumping device; 51, second pipeline; 60, sterilizing filter; 61, sterilizing filter housing; 611, sterilizing filter liquid inlet interface; 612, sterilizing filter liquid outlet interface; 613, sterilizing filter air vent; 70, third pumping device; 71, third pipeline; 80, collector; 81, liquid storage bag; 811, sampling port; 812, collector liquid inlet interface; 82, throttling clamp; 90, fourth pipeline. DETAILED DESCRIPTION

[0027] Embodiments of the present invention are described below with reference to the accompanying drawings. In the following description, many specific details are set forth so that those skilled in the art can more fully understand and implement the present invention. However, it is obvious to those skilled in the art that the implementation of the present invention may not have some of these specific details. In addition, it should be understood that the present invention is not limited to the specific embodiments described. On the contrary, any combination of the features and elements described below may be considered to implement the present invention, regardless of whether they relate to different embodiments. Therefore, the following aspects, features, embodiments and advantages are for illustrative purposes only and should not be regarded as elements or limitations of the claims unless clearly stated in the claims.

[0028] In the following, terms such as "first", "second", etc. are used to describe the elements of the present application. These terms are only used to distinguish the various elements, and are not used to limit the nature, order or number of these elements. The terms "including" and "having" are used to express an open-ended inclusive meaning, and mean that in addition to the listed elements / components, there may be or may be additional elements / components.

[0029] Figure 1 The overall schematic diagram of the device for separating and extracting plant exosomes according to one embodiment of the utility model is shown. Figure 1 As shown, the separation and extraction device according to this embodiment may include a liquid storage container 10, a depth filter 20, a pre-filter 40, a sterilizing filter 60 and a collector 80 connected in series with each other, wherein the liquid storage container 10 may be connected to the depth filter 20 via a first pumping device 30, the depth filter 20 may be connected to the pre-filter 40 via a second pumping device 50, the pre-filter 40 may be connected to the sterilizing filter 60 via a third pumping device 70, and the sterilizing filter 60 may be directly connected to the collector 80. The first pumping device 30, the second pumping device 50 and the third pumping device 70 may be respectively disposed in the first pipeline 31, the second pipeline 51 and the third pipeline 71, and the sterilizing filter 60 may be directly connected to the collector 80 via a fourth pipeline 90.

[0030] Figure 2 A liquid storage container 10 according to an embodiment is shown, which is used to store plant juice. Plant juice refers to a liquid obtained from various plant raw materials (which may be fresh or dried) after specific treatment, from which the desired plant exosomes can be separated and extracted. The specific treatment may, for example, include mixing the washed and dried plant raw materials with an isotonic solution (such as sodium chloride injection) and then crushing them with a wall breaker to obtain a plant residue liquid, stirring the plant residue liquid with a blender for a specific time and then filtering it with a filter to separate the plant juice from the residue and collect it, thereby obtaining the plant juice that can be stored in the liquid storage container 10.

[0031] like Figure 2 As shown, the liquid storage container 10 may include a container body 11 having a plurality of legs. The container body 11 may be stably erected on a supporting surface by means of its plurality of legs, and an outlet for the plant juice to flow out of the liquid storage container 10 may be provided at the center of its bottom. It will be easily understood by those skilled in the art that the above-mentioned supporting surface may be any surface capable of supporting the container body 11, such as a workbench top or the ground. Advantageously, the bottom of the container body 11 is a funnel-shaped structure that tapers downward, and a liquid storage container liquid outlet interface 112 is provided at the bottom end of the funnel-shaped structure as the above-mentioned outlet. This configuration is conducive to causing the plant juice in the container body 11 to flow downward along the inner wall of the bottom of the container and to gather at the liquid storage container liquid outlet interface 112 and flow out, thereby reducing the loss of plant juice. The liquid outlet interface 112 of the liquid storage container is connected to the depth filter 20 via the first pipeline 31 provided with the first pumping device 30, and the plant juice is pumped from the liquid storage container 10 through the liquid storage interface 112 of the liquid storage container and the first pipeline 31 into the depth filter 20 located downstream by the pumping action of the first pumping device 30. In the illustrated embodiment, the liquid storage interface 112 of the liquid storage container is a flange interface, and can be connected to the first pipeline 31 with a corresponding flange connector. The first pumping device 30 can be a peristaltic pump, and the first pipeline 31 is a correspondingly extrudable hose.

[0032] Furthermore, the top of the container body 11 may have an opening 111, so as to inject the plant juice into the container body from the opening 111. The liquid storage container 10 may also include a container cover 12 covering the opening 111. The container cover 12 may be sealedly connected to the container body 11, and an air outlet 121 is provided thereon.

[0033] The liquid storage container 10 may be made of, for example, EVA (ethylene-vinyl acetate copolymer), so as to have good moisture-proof, water-resistant and corrosion-resistant properties. The liquid storage container 10 may have, for example, a capacity of 10 L, and may process a relatively large amount of plant juice at one time.

[0034] Figure 3One embodiment of a depth filter 20 is shown. Those skilled in the art will appreciate that "depth filtration (DF)" as used herein refers to the use of a depth filter medium to remove particles (e.g., impurities) from a liquid such as a solution, by retaining particles and impurities throughout the deep portion of the porous structure of the filter medium through a combination of size (sieving / filtration) and intermolecular interactions (e.g., electrostatic attraction and hydrophobic interactions between oppositely charged surfaces). Depth filtration has been successfully used for the initial clarification of bacterial, yeast, insect, and mammalian cell suspensions, and can be used alone or in combination with centrifugation. The depth filtration medium can include cellulose (e.g., cellulose fibers) and / or polypropylene (e.g., polypropylene fibers), and / or filter aids (e.g., activated carbon, diatomaceous earth (DE), and / or perlite), and / or resins (e.g., polymeric resins). Accordingly, the term "depth filter" as used herein refers to any filter capable of deep filtering a plant juice passing therethrough using a depth filtration medium, such as a depth capsule filter known to those skilled in the art. The depth filter 20 may be a commercially available product or may be homemade.

[0035] exist Figure 3 In the illustrated embodiment, the depth filter 20 may include a vertically arranged depth filter housing 21. A depth filter inlet port 211 and a depth filter outlet port 212 are respectively provided at the top and bottom ends of the depth filter housing 21. The depth filter inlet port 211 is connected to the liquid storage container outlet port 112 via the first pipeline 31. After the plant juice pumped from the liquid storage container 10 enters the depth filter housing 21 via the depth filter inlet port 211, it can flow from top to bottom at least partially by gravity and flow out via the depth filter outlet port 212. The depth filter outlet port 212 is connected to the pre-filter 40 via a second pipeline 51 provided with a second pumping device 50. The plant juice is pumped from the depth filter 20 via the depth filter outlet port 212 and the second pipeline 51 into the pre-filter 40 located downstream by the pumping action of the second pumping device 50. In the illustrated embodiment, the depth filter inlet interface 211 and the depth filter outlet interface 212 are flange interfaces, and can be connected to the first pipeline 31 and the second pipeline 51 with corresponding flange joints respectively. The second pumping device 50 can also be a peristaltic pump, and the second pipeline 51 is a hose that can be squeezed. In addition, the top and bottom of the side wall of the depth filter housing 21 can be respectively provided with a depth filter vent 213 and a depth filter drain 214, both of which can be spiral interfaces with barbs.

[0036] The deep filter 20 may include multiple filter membranes stacked in the deep filter housing 21. The filter membrane may be, for example, a three-dimensional structure composed of cellulose, filter aids (such as diatomaceous earth, perlite, etc.) and synthetic resin. Among them, cellulose constitutes the matrix of the filter membrane and is filled with filter aids to further improve the removal capacity and load of large particle impurities and particles in the plant juice. The filter membrane also uses synthetic resin as a reinforcing agent, which can effectively improve the wet strength, and due to the positive electroactivity of the synthetic resin, it can remove negatively charged pollutants by adsorption, thereby improving the removal effect of impurities. According to a preferred configuration, each layer of the filter membrane may include an upper filter plate and a lower filter plate stacked on each other, wherein the pore size of the upper filter plate is larger than the pore size of the lower filter plate. The upper filter plate with a larger pore size can intercept large-sized particles and play a role in protecting the lower filter plate. The lower filter plate with a smaller pore size further intercepts small particles to ensure the clarity of the filtered liquid. The "upper" and "lower" here refer to the vertically placed deep filter housing 21 of this embodiment, and do not limit the position of itself. In general, the pore size of each filter membrane layer can be in the range of 2 to 30 microns, wherein, for example, the pore size of the upper filter plate is about 11 to 30 microns and the pore size of the lower filter plate is about 6 to 15 microns; or the pore size of the upper filter plate is about 8 to 20 microns and the pore size of the lower filter plate is about 2 to 4 microns.

[0037] Figure 4 An embodiment of a pre-filter 40 is shown. In the present application, a "pre-filter" refers to any filter that is disposed between the aforementioned depth filter and the sterilizing filter described in more detail below in the flow direction of the plant juice and further removes impurities such as particulate matter in the plant juice on the basis of the depth filter. Its main function is to increase the load capacity of the sterilizing filter located downstream thereof, reduce blockage of the sterilizing filter, and extend the service life of the sterilizing filter.

[0038] like Figure 4As shown, the pre-filter 40 may include a pre-filter housing 41 disposed vertically. A pre-filter liquid inlet interface 411 and a pre-filter liquid outlet interface 412 are respectively provided at the top and bottom of the pre-filter housing 41. The pre-filter liquid inlet interface 411 is connected to the deep filter liquid outlet interface 212 via the second pipeline 51. After the plant juice pumped from the deep filter 20 enters the pre-filter housing 41 via the pre-filter liquid inlet interface 411, it can flow from top to bottom at least partially by gravity and flow out via the pre-filter liquid outlet interface 412. The pre-filter liquid outlet interface 412 is connected to the sterilizing filter 60 via the third pipeline 71 provided with the third pumping device 70. The plant juice is pumped from the pre-filter 40 via the pre-filter liquid outlet interface 412 and the third pipeline 71 into the sterilizing filter 60 located downstream by the pumping action of the third pumping device 70. In the illustrated embodiment, the pre-filter liquid inlet interface 411 and the pre-filter liquid outlet interface 412 are flange interfaces, and can be connected to the second pipeline 51 and the third pipeline 71 with corresponding flange joints respectively. The third pumping device 70 can also be a peristaltic pump, and the third pipeline 71 is a hose that can be squeezed. In addition, the top and bottom of the side wall of the pre-filter housing 41 can be respectively provided with a pre-filter vent 413 and a pre-filter drain 414, which can also be spiral interfaces with barbs.

[0039] The pre-filter 40 may include a pre-filter membrane disposed in a folded or stacked manner within the pre-filter housing 41. The pre-filter membrane may be made of, for example, polyethersulfone (PES), which has excellent mechanical properties, high temperature tolerance, corrosion resistance, and high separation efficiency. The pore size of the pre-filter membrane is, for example, 0.4 to 0.8 microns (e.g., 0.45 microns), which is smaller than the pore size of the membrane of the depth filter 20 located upstream.

[0040] Figure 5 One embodiment of a sterilizing filter 60 is shown. In the present application, a "sterilizing filter" refers to a filter that can remove microorganisms such as bacteria from plant juice by physical entrapment and produce a sterile filtrate.

[0041] like Figure 5As shown, the sterilizing filter 60 may include a sterilizing filter housing 61 disposed vertically. A sterilizing filter liquid inlet interface 611 and a sterilizing filter liquid outlet interface 612 are provided at the top and bottom of the sterilizing filter housing 61, respectively. The sterilizing filter liquid inlet interface 611 is connected to the pre-filter liquid outlet interface 412 via the third pipeline 71. The plant juice pumped from the pre-filter 40 enters the sterilizing filter housing 61 via the sterilizing filter liquid inlet interface 611 and can flow from top to bottom at least partially by gravity, and flow out via the sterilizing filter liquid outlet interface 612. The sterilizing filter liquid outlet interface 612 is connected to the collector 80 via the fourth pipeline 90, whereby the sterile plant exosomes obtained after passing through the sterilizing filter can be directly collected and stored in the collector 80. In the illustrated embodiment, the sterilizing filter inlet interface 611 and the sterilizing filter outlet interface 612 are both barbed interfaces. This is because the plant juice flow rate through the sterilizing filter is smaller than that of the deep filter and pre-filter located upstream, so there is no need to use a flange interface with a relatively larger caliber. In addition, a sterilizing filter vent 613 can be provided at the top of the side wall of the sterilizing filter housing 61, which can be a barbed spiral interface.

[0042] The sterilizing filter 60 may include a sterilizing filter membrane disposed in a folded or stacked manner inside the sterilizing filter housing 61. The sterilizing filter membrane may also be made of, for example, polyethersulfone (PES). In order to effectively filter out bacteria, the pore size of the sterilizing filter membrane may be, for example, 0.1 to 0.3 microns (e.g., 0.22 microns), which is further reduced compared to the pore size of the filter membrane of the upstream pre-filter 40.

[0043] In the above embodiments, the deep filter housing 21, the pre-filter housing 41 and the sterilizing filter housing 61 can all be made of polypropylene, which has high impact resistance, strong mechanical properties, and resistance to corrosion by various organic solvents and acids and alkalis.

[0044] In the above embodiment, advantageously, the liquid outlet interface 112 of the liquid storage container, the liquid inlet interface 211 of the deep filter, the liquid outlet interface 212 of the deep filter, the liquid inlet interface 411 of the pre-filter and the liquid outlet interface of the pre-filter may be flange interfaces of the same specification (for example, flange interfaces of 25 mm in specification), and connected to the corresponding joints of the first pipeline 31, the second pipeline 51 and the third pipeline 71 by fixing with flange clamps. In addition, the liquid inlet interface 611 of the sterilizing filter and the liquid outlet interface 612 of the sterilizing filter may also be barbed interfaces of the same specification (for example, barbed interfaces of 1 / 4 inner diameter), and connected to the corresponding joints of the third pipeline 71 and the fourth pipeline 90 by fixing with cable ties. The use of flange interfaces or barbed interfaces of the same specification can improve the versatility of equipment parts (such as pipelines and pipeline joints) and reduce the trouble and cost caused by equipping a variety of parts of different specifications.

[0045] In the above embodiment, the first pumping device 30, the second pumping device 50 and the third pumping device 70 are peristaltic pumps, but they may also be other types of pumps such as diaphragm pumps, gear pumps or centrifugal drive pumps. The pipelines provided with the corresponding pumping devices may be made of different materials (such as soft or hard materials) according to the types of the pumping devices.

[0046] Figure 6 An embodiment of a collector 80 is shown. The collector 80 may include a liquid storage bag 81. The liquid storage bag 81 may have a capacity of up to 5L and may be made of, for example, EVA (such as EVA F1000). Because EVA has good cold resistance and low temperature resistance, such a liquid storage bag is convenient for frozen storage and transportation at a temperature as low as -80°C. The entire liquid storage bag can also be sterilized by GAMA irradiation to ensure the sterility of the plant exosomes collected therein during storage and transportation. The liquid storage bag 81 is provided with a sampling port 811 and a collector liquid inlet interface 812. The sampling port 811 can be inserted into a needle for sampling operation. The collector liquid inlet interface 812 can be connected to the sterilizing filter liquid outlet interface 612 via the fourth pipeline 90. A throttling clamp 82 may also be provided on the fourth pipeline 90 to adjust the flow of plant exosomes during the collection of plant exosomes.

[0047] The following is a brief description of the operation process of the above exemplary separation and extraction device for plant exosomes according to the utility model. First, pour the plant juice obtained or prepared in advance into the liquid storage container 10, and cover the container cover 12 so that it is sealed and fixed with the container body 11. Then, the depth filter vent 213, the pre-filter vent 413 and the sterilizing filter vent 613 are all opened so that the inner cavities of the depth filter 20, the pre-filter 40 and the sterilizing filter 60 can be exhausted to the outside. Next, start the first pumping device 30 to pump the plant juice in the liquid storage container 10 into the deep filter 20 through its pumping action, and close the deep filter vent 213 when the plant juice fills the inner cavity of the deep filter 20; then start the second pumping device 50 to continue pumping the plant juice from the deep filter 20 into the pre-filter 40 through the combined action of the first pumping device 30 and the second pumping device 50, and close the pre-filter vent 413 when the plant juice fills the inner cavity of the pre-filter 40; then start the third pumping device 70 to further pump the plant juice from the pre-filter 40 into the sterilizing filter 60 through the combined action of the first pumping device 30, the second pumping device 50 and the third pumping device 70, and close the sterilizing filter vent 613 when the plant juice fills the inner cavity of the sterilizing filter 60. After all the plant juice has passed through the deep filter 20, the first pumping device 30 is turned off; after all the plant juice has passed through the pre-filter 40, the second pumping device 50 is turned off; and after all the plant juice has passed through the sterilizing filter 60 and entered the collector 80, the third pumping device 70 is turned off. Finally, the throttle clamp 82 provided in the collector 80 is controlled to be completely closed, and the corresponding pipeline is closed using a sterile connector. Thus, the separation and extraction process of plant exosomes is completed using the above equipment.

[0048] The separation and extraction device for plant exosomes of the utility model allows plant juice to continuously pass through a plurality of filters with appropriate filter apertures connected in series, thereby separating and extracting the desired plant exosomes from the plant juice step by step or layer by layer. The separation and extraction of plant exosomes is continuously carried out through this step-by-step filtering device, and the process is simple and efficient. While ensuring the extraction amount and purity of plant exosomes, the separation and extraction time can be greatly shortened, the yield can be significantly increased, the product can be guaranteed to be sterile, and the activity of exosomes is not affected. The separation and extraction device of the utility model has a simple structure, is easy to install, and works stably. There is no need to repeatedly disassemble and assemble parts, and the separated and extracted plant exosomes can be conveniently collected and reliably stored and transported.

[0049] For those skilled in the art, various modifications and variations may be made to the embodiments disclosed above without departing from the scope or spirit of the utility model. Other embodiments of the utility model will be apparent to those skilled in the art from the practice of the utility model disclosed in this specification. This specification and the examples disclosed therein should be considered to be illustrative only, and the true scope of the utility model is specified by the appended claims and their equivalents.

Claims

1. A device for separating and extracting plant exosomes, characterized in that: The separation and extraction equipment comprises: A liquid storage container (10), the liquid storage container being used to store plant juice; a deep filter (20), the deep filter being in communication with the liquid storage container (10) via a first pumping device (30); a pre-filter (40) connected to the depth filter (20) via a second pumping device (50); a sterilizing filter (60) connected to the pre-filter (40) via a third pumping device (70); and A collector (80) is connected to the sterilizing filter (60) to collect the plant exosomes separated and extracted from the plant juice step by step through the depth filter, the pre-filter and the sterilizing filter connected in series.

2. The separation and extraction equipment according to claim 1, characterized in that: The liquid storage container (10) comprises a container body (11) erected on a supporting surface, the bottom of the container body (11) being a funnel-shaped structure that tapers downward, a liquid storage container outlet interface (112) being provided at the bottom end of the funnel-shaped structure, the liquid storage container outlet interface being connected to the deep filter (20) via a first pipeline (31) provided with the first pumping device (30).

3. The separation and extraction equipment according to claim 2, characterized in that: The deep filter (20) comprises a vertically arranged deep filter housing (21), wherein the deep filter housing (21) is provided with a deep filter liquid inlet interface (211) and a deep filter liquid outlet interface (212) at the top and bottom ends respectively, wherein the deep filter liquid inlet interface (211) is connected to the liquid outlet interface (112) of the liquid storage container via the first pipeline (31), and the deep filter liquid outlet interface (212) is connected to the pre-filter (40) via the second pipeline (51) provided with the second pumping device (50).

4. The separation and extraction equipment according to claim 3, characterized in that: The deep filter (20) further comprises a plurality of filter membranes stacked in the deep filter housing (21), each filter membrane having a pore size of 2 to 30 microns, and each filter membrane comprising an upper filter plate and a lower filter plate stacked on top of each other, wherein the pore size of the upper filter plate is larger than the pore size of the lower filter plate.

5. The separation and extraction equipment according to claim 3, characterized in that: The pre-filter (40) comprises a vertically arranged pre-filter housing (41), and the pre-filter housing is provided with a pre-filter liquid inlet interface (411) and a pre-filter liquid outlet interface (412) at the top and bottom ends respectively, the pre-filter liquid inlet interface (411) is connected to the deep filter liquid outlet interface (212) via the second pipeline (51), and the pre-filter liquid outlet interface (412) is connected to the sterilizing filter (60) via a third pipeline (71) provided with the third pumping device (70).

6. The separation and extraction equipment according to claim 5, characterized in that: The pre-filter (40) further comprises a pre-filter membrane arranged in the pre-filter housing (41) in a folded or stacked manner, and the pore size of the pre-filter membrane is 0.4 to 0.8 micrometers.

7. The separation and extraction equipment according to claim 5, characterized in that: The sterilizing filter (60) includes a vertically arranged sterilizing filter housing (61), and the sterilizing filter housing is provided with a sterilizing filter liquid inlet interface (611) and a sterilizing filter liquid outlet interface (612) at the top and bottom ends respectively. The sterilizing filter liquid inlet interface (611) is connected to the pre-filter liquid outlet interface (412) via the third pipeline (71), and the sterilizing filter liquid outlet interface (612) is connected to the collector (80) via the fourth pipeline (90).

8. The separation and extraction equipment according to claim 7, characterized in that: The sterilizing filter (60) further comprises a sterilizing filter membrane which is arranged in a folded or stacked manner in the sterilizing filter housing (61), and the pore size of the sterilizing filter membrane is 0.1 to 0.3 microns.

9. The separation and extraction equipment according to claim 7, characterized in that: The liquid storage container liquid outlet interface (112), the deep filter liquid inlet interface (211), the deep filter liquid outlet interface (212), the pre-filter liquid inlet interface (411) and the pre-filter liquid outlet interface (412) are flange interfaces of the same specification, and the sterilizing filter liquid inlet interface (611) and the sterilizing filter liquid outlet interface (612) are barbed interfaces of the same specification.

10. The separation and extraction equipment according to any one of claims 7 to 9, characterized in that: The collector (80) comprises a liquid storage bag (81) made of EVA, the liquid storage bag being provided with a sampling port (811) and a collector liquid inlet interface (812) connected to the liquid outlet interface (612) of the sterilizing filter via the fourth pipeline (90) with a throttling clamp (82).

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