Handheld fluorescence immunoassay analyzer

Abstract

The utility model discloses a handheld fluorescence immunoassay analyzer. The analyzer comprises a shell, a reagent card positioning and moving module, a code scanning module and a fluorescence detection module. The reagent card positioning and moving module comprises a fixed support, a sliding support, a sliding driving assembly and an in-place detection assembly. The sliding support is driven by a sliding driving assembly to slide. And the sliding bracket is provided with a reagent card slot structure. The in-place detection assembly comprises an in-place detection sensor and an in-place trigger reed. And the in-place detection sensor is arranged on the fixed bracket. And the in-place trigger reed comprises a fixed section, a bent extension section and a trigger section which are connected in sequence. And the fixed section of the in-place trigger reed is fixed with the sliding bracket. A sharp corner part is arranged on the bent extension section; and the sharp corner part extends into the reagent card slot structure. According to the utility model, the in-place detection sensor does not need to be mounted on a sliding bracket, so that the problem that a signal line is repeatedly bent in the working process due to the fact that the in-place detection sensor needs to move is solved.

Description

Technical Field

[0001] The utility model belongs to the technical field of fluorescent immunoassay analyzers, and in particular relates to a handheld fluorescent immunoassay analyzer. Background Art

[0002] Fluorescence immunoassay is a commonly used detection technology in biomedical testing. This technology uses the characteristic that the analyzed substance will produce fluorescence under the stimulation of light of a specific wavelength to perform qualitative and quantitative detection. Due to its high sensitivity, strong specificity, fast detection speed, safety and stability, it is widely used in clinical testing and has broad application prospects in the detection of endocrine diseases, infectious diseases, obstetrics and gynecology, tumor markers, genetic diseases, blood and cytology.

[0003] The basic principle of fluorescence immunoassay technology is that the specific reaction process of the antibody or antigen to be tested is marked by a fluorescent substance. Under the irradiation of excitation light of a specific wavelength, the fluorescent substance will radiate reflected fluorescence of a certain wavelength. The intensity information of the fluorescence is detected by the photoelectric module, and the concentration information of the substance to be tested can be fed back. Fluorescence immunoassay technology has the advantages of strong specificity, high sensitivity, and fast detection speed. Therefore, it is widely used in the fields of microbial detection, viral antigen or antibody detection, hormone detection, tumor marker detection, etc.

[0004] There are many types of fluorescence detection equipment on the market, most of which are desktop devices that cannot meet the needs of mobile detection. Handheld devices have a low degree of automation. Users need to insert the reagent card and then click the start / run button. They also have disadvantages such as low integration and large size.

[0005] The patent with publication number "CN211718304U" provides a miniaturized fluorescent immunochromatography analyzer. To ensure the convenience of reagent card insertion, the limit groove structure size needs to be slightly larger than the reagent card; this will cause the reagent card to shake left and right after insertion, affecting the accuracy of the test results.

[0006] In addition, most of the existing handheld fluorescent immunoassay devices do not have the function of detecting whether the reagent card is in place; and some larger desktop fluorescent immunoassay devices generally install a position sensor on a slot structure that can move back and forth to detect whether the reagent card is inserted in place; because the position sensor needs to move with the slot structure, the signal line of the position sensor will be bent repeatedly during the fluorescence detection process; on the one hand, this requires space to be reserved inside the device for the signal line to bend and move, restricting the miniaturization of the device; on the other hand, the repeated bending of the signal line will cause the service life of the position sensor and its signal line to be reduced, which is an important factor restricting the long-term stability of the fluorescent immunoassay device. Summary of the invention

[0007] The utility model aims to provide a handheld fluorescent immunoassay analyzer.

[0008] The utility model provides a handheld fluorescent immunoassay analyzer, which comprises a shell, and a reagent card positioning and moving module, a code scanning module and a fluorescent detection module which are installed in the shell.

[0009] The reagent card positioning and moving module includes a fixed bracket, a sliding bracket, a sliding drive component and an in-place detection component. The fixed bracket is fixed in the housing; the sliding bracket is slidably connected to the fixed bracket. The sliding bracket is driven by the sliding drive component to slide. The sliding bracket is provided with a reagent card slot structure. The housing is provided with a reagent card insertion port aligned with the reagent card slot structure.

[0010] The reagent card positioning and moving module also includes an in-place detection component; the in-place detection component includes an in-place detection sensor and an in-place trigger spring. The in-place detection sensor is installed on a fixed bracket. The in-place trigger spring includes a fixed section, a bending extension section and a trigger section connected in sequence. The fixed section of the in-place trigger spring is fixed to the sliding bracket. The bending extension section is provided with a sharp corner; the sharp corner extends into the reagent card slot structure.

[0011] The position of the trigger segment matches the position of the in-place detection sensor. When the test agent card is inserted into the reagent card slot structure, the test agent card presses the sharp corner of the in-place trigger reed, causing the in-place trigger reed to deform; the detection state of the in-place detection sensor for the trigger segment changes;

[0012] The code scanning module is used to read the two-dimensional code on the reagent card inserted into the reagent card slot structure; the fluorescence detection module is used to perform fluorescence detection on the reagent card inserted into the reagent card slot structure.

[0013] Preferably, the pointed corner of the bent extension section is located at the inner end of the reagent card slot structure.

[0014] Preferably, in the initial state, the trigger section of the in-place trigger reed is aligned with the detection portion of the in-place detection sensor; when the test reagent card is inserted into the reagent card slot structure, the trigger section of the in-place trigger reed is staggered with the detection portion of the in-place detection sensor.

[0015] Preferably, the length of the trigger section is greater than the stroke of the sliding bracket. In the initial state, when the sliding bracket moves to the two extreme positions, the long strip-shaped trigger section is aligned with the detection part of the in-position detection sensor.

[0016] Preferably, the fixed section of the in-place trigger spring is fixed to the bottom surface of the sliding bracket; the pointed corner of the in-place trigger spring extends into the bottom of the reagent card slot structure through a clearance groove provided on the sliding bracket.

[0017] Preferably, the reagent card positioning and moving module further comprises an extrusion positioning component; the extrusion positioning component is mounted on the sliding bracket and comprises a transverse elastic constraint member and a vertical elastic constraint member; when a reagent card is inserted into the reagent card slot structure, the transverse elastic constraint member and the vertical elastic constraint member respectively press against the reagent card along the width direction and the thickness direction of the reagent card;

[0018] Preferably, the vertical elastic constraint comprises a mounting seat, a top block and a spring. A mounting groove is provided on the sliding bracket. The mounting seat is fixed in the mounting groove; the top block is slidably connected to the mounting seat. The sliding direction of the top block is perpendicular to the top surface of the sliding bracket. A spring is installed between the top block and the bottom of the inner cavity of the mounting seat. In the initial state, the hemispherical top of the top block extends beyond the top surface of the sliding bracket. In the squeezed state, the top block can completely enter the mounting groove.

[0019] Preferably, the transverse elastic restraint comprises one or more elastic sheets, one end of which is fixed to the side of the sliding bracket, and the other end of which extends into the interior of the reagent card slot structure.

[0020] Preferably, guide constraint strips are provided on both sides of the top surface of the sliding bracket. A reagent card slot structure is formed between the two guide constraint strips. A vertical limiter formed by folding inwards is provided on the top of the two guide constraint strips. When the test reagent card is inserted into the reagent card slot structure, the test reagent card is limited in the width direction by the two guide constraint strips. The test reagent card is limited in the thickness direction by the vertical limiter.

[0021] Preferably, the fluorescence detection module includes a main circuit board, a sub-circuit board, a detection base, a fluorescence detection sensor, a filter, an excitation light source, a sealing ring and a compression sealing ring; the main circuit board is fixed in the outer shell; the detection base is fixed to the bottom surface of the circuit board and is located directly above the reagent card slot structure; a first inclined mounting surface is provided on the top of the detection base close to the reagent card insertion port; and the sub-circuit board is fixed on the first inclined mounting surface.

[0022] The detection base is provided with an excitation through hole and a detection through hole. The excitation through hole is vertically arranged, and the top end is connected to the top surface of the detection base; the detection through hole is inclined, and the top end is connected to the first inclined mounting surface. The bottom ends of the excitation through hole and the detection through hole are both facing the same position on the reagent card slot structure.

[0023] The excitation light source is installed on the main circuit board, and the light emitting part extends downward into the excitation through hole. The fluorescence detection sensor is installed on the auxiliary circuit board, and extends downward into the detection through hole.

[0024] The detection through hole is provided with a first step surface and a second step surface. The first step surface and the second step surface divide the detection through hole into a sensor installation section, a filter installation section and a terminal optical path section with decreasing apertures from top to bottom. The detection sensor is installed in the auxiliary circuit board and extends into the sensor installation section. The filter is installed in the filter installation section and is limited by the second step surface. A compression sealing ring is installed in the sensor installation section and surrounds the outside of the detection sensor; the two ends of the compression sealing ring respectively press against the auxiliary circuit board and the first step surface. The inner edge of the compression sealing ring squeezes the edge of the filter.

[0025] Preferably, a sealing ring is provided between the bottom surface of the main circuit board and the top of the excitation through hole.

[0026] Preferably, a correction piece is installed at the bottom end of the excitation through hole.

[0027] Preferably, the scanning module includes a reflector and a scanner; the scanner is fixed in the shell and faces above the reagent card slot structure; a second inclined mounting surface is provided on the side of the bottom of the detection base away from the reagent card insertion port; the reflector is fixed in the shell and is located directly above the reagent card slot structure; the reflector is aligned with the scanning area of ​​the scanner.

[0028] Preferably, a position detection sensor for detecting whether the test reagent card is inserted into place is fixed at the inner end of the fixing bracket.

[0029] Preferably, the handheld fluorescent immunoassay analyzer further comprises a display module, a printer and a card reader module. The display module is mounted on the top surface of the housing and comprises a power button and a touch display screen. The card reader module is mounted in the housing and is close to the inner wall of the housing. A card reader mark is provided on the outer wall of the housing at a position corresponding to the card reader module. The card reader module is used to read the NFC card so that the fluorescent immunoassay analyzer can quickly obtain the batch information of the reagent and the curve information required for calibration of different batches by swiping the card by the user.

[0030] The printer is mounted on the head end of the housing.

[0031] Preferably, the housing comprises an upper cover and a bottom housing connected together by snap fasteners.

[0032] The utility model has the following beneficial effects:

[0033] 1. The utility model installs a position trigger spring that can be moved by the inserted reagent card on the sliding bracket, and installs a position detection sensor on the fixed bracket; the deformation of the position trigger spring before and after being moved is used to change the trigger state of the position detection sensor, and the lateral movement of the position trigger spring with the sliding bracket does not affect the trigger state of the position detection sensor. As a result, the position detection sensor does not need to be installed on the sliding bracket, thereby overcoming the problem that the signal line will be repeatedly bent during operation due to the need to move the position detection sensor, and improving the stability of the handheld fluorescent immunoassay analyzer during long-term use (the bending life of the sensor wire is about 250,000 times, while the life of the elastic spring of many materials can reach millions of deformations).

[0034] 2. The utility model sets the sharp corner of the in-place trigger spring at the inner end of the reagent card slot structure, and the trigger state of the in-place detection sensor will be changed only when the reagent card is inserted to the extreme position, thereby enabling the in-place detection sensor to detect whether the reagent card is not inserted into place.

[0035] 3. Based on the reagent card slot structure, the utility model arranges a spring plunger structure and a spring sheet structure to tightly squeeze the inserted reagent card into the reagent card slot structure; at the same time, a bearing is installed at the end of the screw rod to improve the stability of the reagent card when it moves with the sliding bracket, thereby improving the accuracy of code scanning and detection.

[0036] 4. The utility model introduces a detection base with an excitation through hole and a detection through hole into the fluorescence detection module, and sets a sealing ring and a compression sealing ring at the installation position of the excitation light source and the detection sensor, which can improve the dustproof ability of the fluorescence detection module. At the same time, the compression sealing ring can also play the role of compressing the filter.

[0037] 5. The utility model uses the barcode scanner in conjunction with a reflector to increase the optical path distance between the barcode scanner and the two-dimensional code or barcode in a compact space, thereby improving the success rate and accuracy of barcode scanning. BRIEF DESCRIPTION OF THE DRAWINGS

[0038] Figure 1 It is a schematic diagram of the overall structure of the utility model.

[0039] Figure 2 It is a schematic diagram of the internal structure of the utility model.

[0040] Figure 3 It is a structural schematic diagram of the reagent card positioning and moving module in the utility model.

[0041] Figure 4 It is a top view schematic diagram of the reagent card positioning and moving module in the present utility model.

[0042] Figure 5It is a schematic diagram of the combined structure of the reagent card positioning and moving module, the code scanning module, and the fluorescence detection module in the utility model.

[0043] Figure 6 The schematic diagram of the structure of the fluorescence detection module in the utility model (ie Figure 5 (a partial enlarged view of part A in FIG. 5 ).

[0044] Figure 7 This is a schematic diagram of the process of inserting a reagent card into a reagent card slot in the utility model.

[0045] Figure numerals: 1, housing; 2, reagent card positioning and moving module; 3, code scanning module; 4, fluorescence detection module; 5, display module; 6, printer; 7, card reading module; 8, reagent card to be tested; 1-1, reagent card insertion port; 2-1, fixed bracket; 2-2, sliding bracket; 2-3, guide constraint strip; 2-4, vertical limiter; 2-5, lateral elastic constraint; 2-6, vertical elastic constraint; 2-7, driving bracket; 2-8, motor; 2-9, screw rod; 2-10, guide rod; 2-11, slider; 2-12, rotating bearing; 2-13, in-place detection Sensor; 2-14, in-position trigger reed; 3-1, reflector; 3-2, barcode scanner; 4-1, main circuit board; 4-2, auxiliary circuit board; 4-3, detection base; 4-4, fluorescence detection sensor; 4-5, filter; 4-6, excitation light source; 4-7, sealing ring; 4-8, compression sealing ring; 4-9, excitation through hole; 4-10, detection through hole; 2-2-1, make way slot 2-14-1, fixed section; 2-14-2, bending extension section; 2-14-3, trigger section; 4-10-1, first step surface; 4-10-2, second step surface. DETAILED DESCRIPTION

[0046] The utility model is further described below in conjunction with the accompanying drawings.

[0047] like Figure 1 and 2 As shown, a handheld fluorescent immunoassay analyzer includes a housing 1, and a reagent card positioning and moving module 2, a code scanning module 3, a fluorescent detection module 4, a display module 5, a printer 6 and a card reading module 7 installed on the housing 1.

[0048] The housing 1 includes an upper cover and a bottom housing connected together by a buckle. The display module 5 is installed on the top surface of the upper cover and includes a power button and a touch display screen. The card reader module 7 is installed in the housing 1 and close to the inner wall of the housing 1. A card reader mark is provided on the outer wall of the housing 1 at a position corresponding to the card reader module 7. The printer 6 is installed at the head end of the housing 1. The reagent card positioning and moving module 2, the code scanning module 3 and the fluorescence detection module 4 are all installed inside the housing 1.

[0049] like Figure 3 and 4 As shown, the reagent card positioning and moving module 2 includes a fixed bracket 2-1, a sliding bracket 2-2, an extrusion positioning component, a sliding drive component and an in-place detection component. The fixed bracket 2-1 is fixed in the housing 1; the sliding bracket 2-2 is slidably connected to the slide groove on the fixed bracket 2-1. The sliding bracket 2-2 is driven by the sliding drive component to slide.

[0050] Both sides of the top surface of the sliding bracket 2-2 are provided with guide constraint strips 2-3. A reagent card slot structure is formed between the two guide constraint strips 2-3. The spacing between the adjacent sides of the two guide constraint strips 2-3 is slightly larger than the width of the tested reagent card 8. The tops of the two guide constraint strips 2-3 are provided with vertical limiter portions 2-4 formed by folding inward. The distance between the vertical limiter portion 2-4 and the top surface of the sliding bracket 2-2 is slightly larger than the thickness of the tested reagent card 8. A reagent card insertion port 1-1 is provided at the tail end of the housing 1. The reagent card insertion port 1-1 is aligned with the reagent card slot structure on the sliding bracket 2-2. The user can insert the reagent card to be tested into the reagent card slot structure through the reagent card insertion port 1-1.

[0051] like Figure 5 As shown, the in-place detection component is used to detect whether the reagent card to be tested is inserted in place, which includes an in-place detection sensor 2-13 and an in-place trigger reed 2-14. The in-place detection sensor 2-13 is installed at the bottom of the fixed bracket 2-1. The inner end of the sliding bracket 2-2 is provided with a clearance groove 2-2-1. The top of the clearance groove 2-2-1 is connected to the inner end of the reagent card slot structure. The clearance groove 2-2-1 is located directly above the bottom hollow structure of the fixed bracket 2-1.

[0052] The in-place trigger spring 2-14 can bend and deform when subjected to external force, and includes a fixed section 2-14-1, a bent extension section 2-14-2 and a trigger section 2-14-3 connected in sequence. The fixed section 2-14-1 of the in-place trigger spring 2-14 is fixed to the inner end of the bottom surface of the sliding bracket 2-2. The bent extension section 2-14-2 includes a first extension section and a second extension section connected at right angles. The first extension section takes the end of the fixed section 2-14-1 as a starting point, obliquely passes through the clearance groove 2-2-1 on the sliding bracket 2-2, and extends into the reagent card slot structure. The second extension section takes the end of the first extension section as a starting point, obliquely passes through the long strip of clearance groove 2-2-1 on the sliding bracket 2-2 and the bottom hollow structure of the fixed bracket 2-1 on the sliding bracket 2-2, and the end is connected to the inner end of the trigger section 2-14-3. The outer end of the trigger section 2-14-3 is suspended. The length of the trigger section 2-14-3 is greater than the stroke of the sliding bracket. In the entire stroke of the sliding bracket 2-2, the in-place trigger reed 2-14 will not touch the fixed bracket 2-1.

[0053] In the initial state, the trigger segment 2-14-3 is aligned with the detection portion of the in-position detection sensor 2-13, and the in-position detection sensor 2-13 can detect the trigger segment 2-14-3. The connection between the first extension segment and the second extension segment forms an upward pointed corner. Figure 7 As shown, when the test agent card is inserted to the extreme position, the sharp corner of the bent extension section 2-14-2 is squeezed downward, causing the bent extension section 2-14-2 to undergo elastic deformation as a whole, so that the trigger section 2-14-3 is offset from the detection section of the in-place detection sensor 2-13, and the in-place detection sensor 2-13 cannot detect the trigger section 2-14-3; therefore, whether the test agent card 8 is inserted in place can be judged based on whether the in-place detection sensor 2-13 detects the trigger section 2-14-3.

[0054] In the initial state, when the sliding bracket moves to the two extreme positions, the long strip trigger section 2-14-3 is aligned with the detection part of the in-place detection sensor 2-13. Therefore, whether the in-place detection sensor 2-13 stops being triggered is only related to whether the test agent card 8 is inserted in place, and has nothing to do with other factors.

[0055] In this embodiment, the in-place detection sensor 2-13 is a U-shaped photoelectric sensor with the opening facing downward; in some other embodiments, a trigger switch or other sensor capable of detecting the trigger segment 2-14-3 of the in-place trigger reed 2-14 is used.

[0056] like Figure 3 and 4 As shown, the extrusion positioning assembly is installed on the sliding bracket 2-2, including a transverse elastic constraint 2-5 and a vertical elastic constraint 2-6. The vertical elastic constraint 2-6 includes a mounting seat, a top block and a spring. The top surface of the sliding bracket 2-2 is provided with a plurality of mounting grooves. The mounting grooves are arranged in sequence along the length direction of the reagent card slot structure. The mounting seat is fixed in the mounting groove; the top block is slidably connected to the mounting seat. The sliding direction of the top block is perpendicular to the top surface of the sliding bracket 2-2. A spring is installed between the top block and the bottom of the inner cavity of the mounting seat. In the initial state, the hemispherical top of the top block extends beyond the top surface of the sliding bracket 2-2. When squeezed, the top block can completely enter the mounting groove.

[0057] like Figure 3 and 4 As shown, the transverse elastic constraint 2-5 includes one or more elastic sheets. One end of the elastic sheet is fixed to the outer side of one of the guide constraint strips 2-3. The other end of the elastic sheet obliquely extends into one side of the reagent card slot structure.

[0058] When a test reagent card 8 is inserted into the reagent card slot structure, the top block of the vertical elastic constraint 2-6 presses against the back of the test reagent card 8, so that the two sides of the front of the test reagent card 8 are tightly fitted on the vertical limit parts 2-4 of the two guide constraint strips 2-3; the end of the elastic sheet in the transverse elastic constraint 2-5 presses against one side edge of the test reagent card 8, so that the other side edge of the test reagent card 8 is tightly fitted on the guide constraint strip 2-3 of the non-set transverse elastic constraint 2-5. In this way, the stability of the connection between the test reagent card 8 and the sliding bracket 2-2 is ensured to be relatively still.

[0059] like Figure 3 As shown, the sliding drive assembly includes a driving bracket 2-7, a motor 2-8, a screw 2-9, a guide rod 2-10, a slider 2-11 and a rotating bearing 2-12. The driving bracket 2-7 is fixed to the side of the fixed bracket 2-1. The guide rod 2-10 is fixed to the driving bracket 2-7. One end of the screw 2-9 is connected to one end of the driving bracket 2-7 through a rotating bearing 2-12. The motor 2-8 is fixed to the other end of the driving bracket 2-7, and the output shaft is fixed to the other end of the screw 2-9. The slider 2-11 is connected to the guide rod 2-10 through a linear bearing. The nut fixed on the slider 2-11 and the screw 2-9 form a spiral pair. The slider 2-11 is fixed to the sliding bracket 2-2. In this embodiment, the sliding bracket 2-2 and the slider 2-11 are an integrally formed structure.

[0060] like Figure 5 As shown, the fluorescence detection module 4 comprises a main circuit board 4-1, a sub-circuit board 4-2, a detection base 4-3, a fluorescence detection sensor 4-4, a filter 4-5, an excitation light source 4-6, a sealing ring 4-7 and a compression sealing ring 4-8. The main circuit board 4-1 is fixed in the housing 1, and is arranged above the reagent card positioning and moving module 2 at intervals. The detection base 4-3 is fixed on the bottom surface of the circuit board and is located directly above the reagent card slot structure. The top of the detection base 4-3 is provided with a first inclined mounting surface near one side of the reagent card insertion port 1-1. The sub-circuit board 4-2 is fixed on the first inclined mounting surface.

[0061] like Figure 6 As shown, an excitation through hole 4-9 and a detection through hole 4-10 are provided on the detection base 4-3. The excitation through hole 4-9 is vertically arranged, and the top end is connected to the top surface of the detection base 4-3; the detection through hole 4-10 is inclined, and the top end is connected to the first inclined mounting surface. The bottom ends of the excitation through hole 4-9 and the detection through hole 4-10 are both facing the same position on the reagent card slot structure. A correction piece is provided at the bottom end of the excitation through hole 4-9. The correction piece is used to present a strip-shaped light spot when the excitation light is hit on the reaction strip of the reagent card.

[0062] The excitation light source 4-6 is mounted on the main circuit board 4-1, and the light-emitting portion extends downward into the excitation through hole 4-9. A sealing ring 4-7 is provided between the bottom surface of the main circuit board 4-1 and the stepped surface at the top of the inner cavity of the excitation through hole 4-9. In some embodiments, a lens is also fixed to the middle of the excitation through hole 4-9 through the stepped surface to prevent dust and stray light.

[0063] The fluorescence detection sensor 4-4 is mounted on the auxiliary circuit board 4-2 and extends downward into the detection through hole 4-10. A first step surface 4-10-1 and a second step surface 4-10-2 are provided in the detection through hole 4-10. The first step surface 4-10-1 and the second step surface 4-10-2 divide the detection through hole 4-10 into a sensor mounting section, a filter mounting section and a terminal optical path section with decreasing apertures from top to bottom. The detection sensor 4-4 is located in the sensor mounting section. The filter 4-5 is mounted in the filter mounting section and is limited by the second step surface 4-10-2. The thickness of the filter 4-5 is greater than or equal to the axial dimension of the filter mounting section. The detection portion of the fluorescence detection sensor 4-4 is aligned with the filter 4-5.

[0064] After installation in the sensor installation section, a compression sealing ring 4-8 surrounds the outside of the detection sensor 4-4; the two ends of the compression sealing ring 4-8 respectively press against the bottom surface of the auxiliary circuit board 4-2 and the first step surface 4-10-1 in the detection through hole 4-10. The inner diameter of the compression sealing ring 4-8 is smaller than the diameter of the filter 4-5, so that the inner edge of the compression sealing ring 4-8 squeezes the edge of the filter 4-5, thereby fixing the position of the filter 4-5 by pressure. Therefore, the compression sealing ring 4-8 can prevent dust from entering the detection through hole 4-10, and can also fix the position of the filter 4-5.

[0065] The bottom surface of the auxiliary circuit board 4-2, the compression seal ring 4-8 and the filter 4-5 cooperate to form a dustproof chamber. The detection sensor 4-4 is installed in the dustproof chamber, which can effectively improve the stability of the detection sensor 4-4 in long-term use.

[0066] like Figure 5As shown, the code scanning module 3 includes a reflector 3-1 and a code scanner 3-2. The code scanner 3-2 is fixed to the end of the fixed bracket 2-1 away from the reagent card insertion port 1-1, and toward the top of the reagent card slot structure; the direction of the code scanner 3-2 is parallel to the length direction of the reagent card slot structure; the bottom of the detection base 4-3 is away from the reagent card insertion port 1-1. The side is provided with a second inclined mounting surface. The reflector 3-1 is fixed on the second inclined mounting surface and is located directly above the reagent card slot structure. The reflector 3-1 is aligned with the code scanning area of ​​the code scanner 3-2, and the mirror surface of the reflector 3-1 is at a 45° angle to the direction of the code scanner 3-2. Driven by the sliding drive assembly, the QR code or barcode on the tested agent card 8 inserted into the reagent card slot structure can pass directly below the reflector 3-1, so that the code scanner 3-2 can use the reflector 3-1 to scan the QR code or barcode. The code scanning module 3 based on the reflective structure can be arranged in a horizontal orientation, and can use the length space of the analyzer to increase the distance between the code scanner 3-2 and the two-dimensional code or barcode, thereby maintaining the success rate and accuracy of code scanning in a compact space.

[0067] The working principle of the handheld fluorescent immunoassay analyzer is as follows:

[0068] First, if Figure 7 As shown, the reagent card 8 to be tested is inserted into the reagent card insertion port 1-1 to the limit position, and the in-place trigger reed 2-14 is squeezed by the reagent card 8 and deformed; the detection state of the in-place detection sensor 2-13 changes.

[0069] Next, the sliding drive assembly drives the sliding bracket 2-2 to slide, so that the barcode or QR code on the test agent card 8 slides to the bottom of the reflector 3-1; the scanner 3-2 uses the reflector 3-1 to scan the QR code or barcode to obtain the user, batch and other information corresponding to the test agent card 8.

[0070] Then, the sliding drive assembly drives the sliding bracket 2-2 to slide, so that the fluorescence reading area of ​​the tested agent card 8 moves to the right below the excitation through hole 4-9. The excitation light source 4-6 emits light to illuminate the fluorescence reading area of ​​the tested agent card 8; the fluorescence detection sensor 4-4 reads the fluorescence emission of the tested agent card 8 to obtain the detection result. The display module 5 displays the detection result; the printer 6 prints out the paper with the detection result.

[0071] Finally, the test reagent card 8 is pulled out from the reagent card insertion port 1-1.

Claims

1. A handheld fluorescent immunoassay analyzer, comprising a housing (1), and a reagent card positioning and moving module (2), a code scanning module (3) and a fluorescent detection module (4) installed in the housing (1); characterized in that: The reagent card positioning and moving module (2) comprises a fixed bracket (2-1), a sliding bracket (2-2), a sliding drive component and an in-position detection component; the fixed bracket (2-1) is fixed in the housing (1); the sliding bracket (2-2) is slidably connected to the fixed bracket (2-1); the sliding bracket (2-2) is driven by the sliding drive component to slide; the sliding bracket (2-2) is provided with a reagent card slot structure; the housing (1) is provided with a reagent card insertion port (1-1) aligned with the reagent card slot structure; The reagent card positioning and moving module (2) further comprises an in-place detection component; the in-place detection component comprises an in-place detection sensor (2-13) and an in-place trigger spring (2-14); the in-place detection sensor (2-13) is mounted on a fixed bracket (2-1); the in-place trigger spring (2-14) comprises a fixed section (2-14-1), a bent extension section (2-14-2) and a trigger section (2-14-3) connected in sequence; the fixed section (2-14-1) of the in-place trigger spring (2-14) is fixed to the sliding bracket (2-2); a sharp corner portion is provided on the bent extension section (2-14-2); the sharp corner portion extends into the reagent card slot structure; The position of the trigger segment (2-14-3) matches the position of the in-place detection sensor (2-13); when the test agent card (8) is inserted into the reagent card slot structure, the test agent card (8) presses the sharp corner of the in-place trigger reed (2-14), causing the in-place trigger reed (2-14) to deform; and the detection state of the in-place detection sensor (2-13) on the trigger segment (2-14-3) changes; The code scanning module (3) is used to read the two-dimensional code on the reagent card inserted into the reagent card slot structure; the fluorescence detection module (4) is used to perform fluorescence detection on the reagent card inserted into the reagent card slot structure.

2. A handheld fluorescent immunoassay analyzer according to claim 1, characterized in that: The pointed corner of the bent extension section (2-14-2) is located at the inner end of the reagent card slot structure.

3. A handheld fluorescent immunoassay analyzer according to claim 1, characterized in that: In an initial state, the trigger section (2-14-3) of the in-place trigger reed (2-14) is aligned with the detection portion of the in-place detection sensor (2-13); when the test reagent card (8) is inserted into the reagent card slot structure, the trigger section (2-14-3) of the in-place trigger reed (2-14) is staggered with the detection portion of the in-place detection sensor (2-13).

4. A handheld fluorescent immunoassay analyzer according to claim 1, characterized in that: The fixed section (2-14-1) of the in-position trigger spring (2-14) is fixed to the bottom surface of the sliding bracket (2-2); the pointed corner on the in-position trigger spring (2-14) extends into the bottom of the reagent card slot structure through a clearance groove provided on the sliding bracket (2-2).

5. A handheld fluorescent immunoassay analyzer according to claim 1, characterized in that: The reagent card positioning and moving module (2) further comprises an extrusion positioning component; the extrusion positioning component is mounted on the sliding bracket (2-2) and comprises a transverse elastic constraint member (2-5) and a vertical elastic constraint member (2-6); when a reagent card is inserted into the reagent card slot structure, the transverse elastic constraint member (2-5) and the vertical elastic constraint member (2-6) respectively press against the reagent card along the width direction and thickness direction of the reagent card.

6. A handheld fluorescent immunoassay analyzer according to claim 5, characterized in that: The vertical elastic restraint (2-6) comprises a mounting seat, a top block and a spring; a mounting groove is provided on the sliding bracket (2-2); the mounting seat is fixed in the mounting groove; the top block is slidably connected to the mounting seat; the sliding direction of the top block is perpendicular to the top surface of the sliding bracket (2-2); a spring is installed between the top block and the bottom of the inner cavity of the mounting seat; in an initial state, the top of the top block is hemispherical and extends beyond the top surface of the sliding bracket (2-2); in a squeezed state, the top block can completely enter the mounting groove; The transverse elastic restraint (2-5) comprises one or more elastic sheets; one end of the elastic sheet is fixed to the side of the sliding bracket (2-2); and the other end of the elastic sheet extends into the interior of the reagent card slot structure.

7. A handheld fluorescent immunoassay analyzer according to claim 1, characterized in that: The fluorescence detection module (4) comprises a main circuit board (4-1), a sub-circuit board (4-2), a detection base (4-3), a fluorescence detection sensor (4-4), a filter (4-5), an excitation light source (4-6), a sealing ring (4-7) and a compression sealing ring (4-8); the main circuit board (4-1) is fixed in the housing (1); the detection base (4-3) is fixed on the bottom surface of the circuit board and is located directly above the reagent card slot structure; a first inclined mounting surface is provided on the top of the detection base (4-3) near the reagent card insertion port (1-1); and the sub-circuit board (4-2) is fixed on the first inclined mounting surface; The detection base (4-3) is provided with an excitation through hole (4-9) and a detection through hole (4-10); the excitation through hole (4-9) is vertically arranged, and the top end is connected to the top surface of the detection base (4-3); the detection through hole (4-10) is inclinedly arranged, and the top end is connected to the first inclined mounting surface; the bottom ends of the excitation through hole (4-9) and the detection through hole (4-10) are both oriented to the same position on the reagent card slot structure; The excitation light source (4-6) is mounted on the main circuit board (4-1), and the light-emitting portion extends downward into the excitation through hole (4-9); the fluorescence detection sensor (4-4) is mounted on the auxiliary circuit board (4-2), and extends downward into the detection through hole (4-10); The detection through hole (4-10) is provided with a first stepped surface (4-10-1) and a second stepped surface (4-10-2); the first stepped surface (4-10-1) and the second stepped surface (4-10-2) divide the detection through hole (4-10) into a sensor installation section, a filter installation section and a terminal optical path section with apertures decreasing from top to bottom; the detection sensor (4-4) is installed in the auxiliary circuit board (4-2) and extends into the sensor installation section; the filter (4-5) is installed in the filter installation section and is limited by the second stepped surface (4-10-2); a compression sealing ring (4-8) is installed in the sensor installation section and surrounds the outer side of the detection sensor (4-4); the two ends of the compression sealing ring (4-8) respectively abut against the auxiliary circuit board (4-2) and the first stepped surface (4-10-1); the inner edge of the compression sealing ring (4-8) presses the edge of the filter (4-5).

8. A handheld fluorescent immunoassay analyzer according to claim 7, characterized in that: The code scanning module (3) comprises a reflector (3-1) and a code scanner (3-2); the code scanner (3-2) is fixed in the housing (1) and faces the upper side of the reagent card slot structure; a second inclined mounting surface is provided at the bottom of the detection base (4-3) on the side away from the reagent card insertion port (1-1); the reflector (3-1) is fixed in the housing (1) and is located directly above the reagent card slot structure; the reflector (3-1) is aligned with the code scanning area of ​​the code scanner (3-2).

9. A handheld fluorescent immunoassay analyzer according to claim 1, characterized in that: The sliding drive assembly comprises a driving bracket (2-7), a motor (2-8), a screw rod (2-9), a guide rod (2-10), a slider (2-11) and a rotating bearing (2-12); the driving bracket (2-7) is fixed to the side of the fixed bracket (2-1); the guide rod (2-10) is fixed to the driving bracket (2-7); one end of the screw rod (2-9) is connected to one end of the driving bracket (2-7) through the rotating bearing (2-12); the motor (2-8) is fixed to the other end of the driving bracket (2-7), and the output shaft is fixed to the other end of the screw rod (2-9); the slider (2-11) is slidingly connected to the guide rod (2-10); a nut fixed to the slider (2-11) and the screw rod (2-9) form a spiral pair; the slider (2-11) is fixed to the sliding bracket (2-2).

10. A handheld fluorescent immunoassay analyzer according to claim 1, characterized in that: The handheld fluorescent immunoassay analyzer further comprises a display module (5), a printer (6) and a card reading module (7); the display module (5) is mounted on the top surface of the housing and comprises a power button and a touch display screen; the card reading module (7) is mounted inside the housing (1) and close to the inner wall of the housing (1); a card reading mark is provided on the outer wall of the housing (1) at a position corresponding to the card reading module (7); and the printer (6) is mounted at the head end of the housing (1).

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