Immunoassay microwell plate sample adding auxiliary device

Abstract

The utility model discloses an immunoassay microplate sample adding auxiliary device which comprises a bottom plate and a movable frame, the movable frame is slidably clamped on a pair of guide plates arranged on the top surface of the bottom plate, a linkage mechanism is arranged on the movable frame and comprises a stand column slidably clamped on the movable frame, and a fixing ring is arranged at one end of the stand column. A sliding rod is clamped at the top end of the stand column in a sliding mode, a wedge-shaped block is arranged at one end of the sliding rod, the slope side of the wedge-shaped block makes contact with one side of the pressing block, the wedge-shaped block is connected with the stand column through a first spring, and a pair of connecting rods is hinged to the free end of the sliding rod; the bottom ends of the two connecting rods are jointly hinged to a driving frame. The linkage assembly can automatically adjust the sample adding position of the sample adding assembly after an inspector operates the sample adding assembly to carry out one-time sample adding, so that the inspector does not need to manually calibrate the position of the sample adding assembly to carry out sample adding after each time of sample adding, and the operation efficiency of the inspector is improved.

Description

Technical Field

[0001] The invention relates to the technical field of experimental equipment, and more specifically, to an auxiliary device for adding samples to a microplate of an immunoassay. Background Art

[0002] Medical testing is a science that conducts microbiological, immunological, cytological and other tests on materials taken from the human body to provide information for the prevention, diagnosis and treatment of human diseases. Among them, the test of human immune function is called immunoassay. In this type of test process, microplates are usually used as carriers for experimental samples.

[0003] However, most of the existing microplate sample loading and testing auxiliary devices are automatic fixed-distance sample loading. Such equipment is usually costly and heavy, and is inconvenient to move or carry. Long-term manual control of sample loading will bring a heavy burden to the inspectors, reducing their work efficiency. Summary of the invention

[0004] The present invention provides an auxiliary device for sample loading on a microplate of an immunoassay to solve the problem that most of the auxiliary devices for sample loading on a microplate in the prior art are automatic fixed-distance sample loading, which is usually costly and heavy, and inconvenient to move or carry, while long-term manual control of sample loading will bring a heavy burden to the inspectors, thereby reducing the work efficiency of the inspectors.

[0005] In order to achieve the above object, the present invention provides the following technical solutions:

[0006] A microplate loading auxiliary device for immunoassay comprises a base plate and a movable frame, wherein the movable frame is slidably mounted on a pair of guide plates arranged on the top surface of the base plate, and a linkage assembly is arranged on the movable frame, and the linkage assembly comprises a column slidably mounted on the movable frame, a fixing ring is arranged at one end of the column, a control ring is rotatably mounted at the bottom end of the fixing ring, a loading assembly is arranged on the control ring, a pressure block is arranged at the bottom end of the control ring, a sliding rod is slidably mounted on the top end of the column, a wedge block is arranged at one end of the sliding rod, the inclined side of the wedge block contacts with one side of the pressure block, the wedge block is connected to the column by a first spring, a pair of connecting rods are hinged at the free end of the sliding rod, and the bottom ends of the two connecting rods are hinged to a driving frame.

[0007] Preferably, a connecting block is rotatably provided on the column, a gear is provided on the connecting block, a driving shaft is eccentrically provided on one side of the connecting block, the driving shaft is provided in the driving frame, a rack is slidably provided on the movable frame through a T-shaped block, and the rack is meshed with the gear.

[0008] Preferably, the sample loading assembly includes a pair of guide frames arranged on the control ring, and a pair of guide frames are symmetrically arranged on the fixed ring. Sliding blocks are slidably clamped in the two guide frames, and the top of the sliding block is connected to a clamping plate. The bottom ends of the two sliding blocks are respectively clamped in the two guide frames.

[0009] Preferably, a control rod is rotatably provided on the clamping ring connected to the outside of the control ring, a first end plate is provided at the bottom end of the clamping ring, a second end plate is provided at the bottom end of the fixing ring, an arc rod provided on the first end plate is passed through a fixing sleeve provided on the second end plate, and a second spring is provided between the first end plate and the second end plate.

[0010] Preferably, a calibration ring connected via a connecting frame is provided at the bottom end of the fixing ring, a radiation calibrator is provided on the calibration ring, and the axis of the calibration ring and the focal point of the radiation calibrator are both located on the same vertical line as the axis of the fixing ring.

[0011] Preferably, a fixing component is provided on the movable frame, and the fixing component includes a connecting arm which is clamped on the rotating block on the movable frame, a fixed frame is provided at one end of the connecting arm, a positioning cylinder is slidably clamped in the fixed frame, a pulling plate connected by a connecting rod is provided on the inner wall of the positioning cylinder, one end of the inner wall of the positioning cylinder is connected to the fixed frame by a third spring, and a side plate arranged on the outer side of the positioning cylinder is slidably clamped in the fixed frame.

[0012] Preferably, a distance adjustment component is provided on the base plate, and the distance adjustment component includes a screw threaded on the base plate, one end of the screw is coaxially connected to a rotating plate, one end of the screw is rotatably clamped on one side of the movable frame, and one end of the base plate is provided with a ruler parallel to the axis of the screw.

[0013] The principle and beneficial effects of this technical solution:

[0014] (1) The linkage assembly provided in the present invention can automatically adjust the sample loading position of the sample loading assembly after the inspector operates the sample loading assembly to load the sample once, so that the inspector does not need to manually calibrate the position of the sample loading assembly after each loading, and can repeat the above operation when dripping the next microwell. The user can adapt to different microwell plates by replacing the gear and the rack, thereby improving the universality of the device. The device can automatically adjust the dripping position of the dropper after the user drips the sample, thereby improving the work efficiency of the inspector.

[0015] (2) The sample adding assembly provided in the present invention can help the inspector simplify the dripping operation. When the sample needs to be added, the initial position of the sample adding assembly is first calibrated through the calibration ring, and then the dropper is placed. Then, the control rod connected to the outer side of the control ring through the clamping ring is turned. When the control ring is rotated 90 degrees to reduce the distance between the clamping plates to the minimum value, the dropper will drip out the sample.

[0016] (3) The fixing assembly provided in the present invention can fix the rack. When the user needs to change the specifications of the gear and the rack, the pull plate can be pulled outward. At this time, the pull plate will compress the third spring and make the connecting rod slide along the fixed frame. When the fixed cylinder is disengaged from the clamping device of the movable frame, the rack can be taken out by rotating the connecting arm along the movable frame, and the specifications of the rack and the gear can be changed synchronously. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a schematic diagram of the structure after assembly of the present invention;

[0018] Figure 2 for Figure 1 Schematic diagram of the enlarged structure of the A area in the middle;

[0019] Figure 3 It is a schematic diagram of the structure after the present invention is split;

[0020] Figure 4 for Figure 3 Schematic diagram of the enlarged structure of the middle B area;

[0021] Figure 5 for Figure 3 Schematic diagram of the enlarged structure of the middle C region;

[0022] Figure 6 It is a schematic diagram of the overall structure of the present invention from another perspective;

[0023] Figure 7 for Figure 6 Schematic diagram of the enlarged structure of the D region in the middle;

[0024] The figure marks in the drawings of the specification include: 1, base plate; 2, ruler; 3, moving frame; 4, rack; 5, guide plate; 6, column; 7, second end plate; 8, fixed sleeve; 9, clamping plate; 10, sliding block; 11, guide frame; 12, control rod; 13, fixing ring; 14, driving shaft; 15, calibration ring; 16, control ring; 17, second spring; 18, pressure block; 19, connecting frame; 20, guide frame; 22, snap ring; 23, first end plate; 24, arc rod; 25, positioning cylinder; 26, third spring; 27, pull plate; 28, connecting rod; 29, side plate; 30, connecting arm; 31, fixed frame; 32, T-block; 33, screw; 34, rotating plate; 35, sliding rod; 36, gear; 37, connecting block; 38, wedge block; 39, first spring; 40, connecting rod; 41, driving frame. DETAILED DESCRIPTION

[0025] The present invention is further described in detail below in conjunction with the accompanying drawings and embodiments:

[0026] Example:

[0027] like Figures 1 to 7 As shown, the present invention provides an immunoassay microplate loading auxiliary device, comprising a base plate 1 and a moving frame 3, the moving frame 3 is slidably clamped on a pair of guide plates 5 arranged on the top surface of the base plate 1, a linkage component is arranged on the moving frame 3, the linkage component comprises a column 6 slidably clamped on the moving frame 3, a fixed ring 13 is arranged at one end of the column 6, a control ring 16 is rotatably clamped at the bottom end of the fixed ring 13, a loading component is arranged on the control ring 16, a pressing block 18 is arranged at the bottom end of the control ring 16, a sliding rod 35 is slidably clamped at the top end of the column 6, a wedge block 38 is arranged at one end of the sliding rod 35, the inclined side of the wedge block 38 is in contact with one side of the pressing block 18, the wedge block 38 is connected to the column 6 by a first spring 39, a pair of connecting rods 40 are hinged at the free end of the sliding rod 35, and a driving frame 41 is hinged at the bottom ends of the two connecting rods 40.

[0028] like Figure 1 , Figure 5 and Figure 7 As shown, a connecting block 37 is rotatably provided on the column 6, a gear 36 is provided on the connecting block 37, a driving shaft 14 is eccentrically provided on one side of the connecting block 37, the driving shaft 14 is clamped in the driving frame 41, and a rack 4 is slidably clamped on the movable frame 3 through a T-shaped block 32, and the rack 4 is meshed with the gear 36.

[0029] The linkage component can automatically adjust the sample loading position of the sample loading component after the inspector operates the sample loading component to load the sample once, so that the inspector does not need to manually calibrate the position of the sample loading component after each loading, thereby improving the operating efficiency of the inspector. Before using the sample loading component, the inspector needs to move the microplate first, so that the axis of the calibration ring 15 in the sample loading component and the focal point of the radiation calibrator are aligned with the middle of the two microwells to be loaded with samples. At this time, the fixed ring 13 is also synchronously moved to the middle of the two microwells, and then the dropper is placed in the fixed ring 13, so that the dropper is inserted into the calibration ring 15, and then the The user can toggle the control rod 12 in the sample addition assembly until the clamping plate 9 set on the fixed ring 13 squeezes the dropper until the dropper drips out the sample. At this time, the rotation angle of the control ring 16 is the maximum value. At the same time, the pressure block 18 set at the bottom end of the control ring 16 will squeeze the inclined side of the wedge block 38, so that the wedge block 38 moves backward and squeezes the slide bar 35, so that the slide bar 35 slides along the top of the column 6, and at the same time compresses the first spring 39 set between the wedge block 38 and the column 6. Because the bottom ends of a pair of connecting rods 40 hinged at the free end of the slide bar 35 are jointly hinged with a driving frame, and the driving frame is provided with an eccentric spring 39 between the connecting block 37. The drive shaft 14 is set, wherein the initial position of the axis of the drive shaft 14 is not located in the same vertical plane as the axis of the connecting block 37. Therefore, when the slide bar 35 slides along the column 6 to drive the drive frame to move downward, the drive frame will squeeze the drive shaft 14, so that the connecting block 37 where the drive shaft 14 is located drives the gear 36 to rotate, thereby causing the gear 36 to roll along the rack 4, driving the column 6 to translate. When the control ring 16 rotates at the maximum angle, the drive shaft 14 rotates 180 degrees, at which time the spacing between the splints 9 becomes the minimum value, and at the same time, the fixed ring 13 moves to the top of the micropore under the drive of the gear 36, completing the sample After dripping, release the control rod 12, the first spring 39 will drive the wedge block 38 to reset, and the driving frame will move up under the pull of the slide bar 35, so that the driving shaft 14 returns to the initial position along the same rotation direction. At this time, the gear 36 rotates 360 degrees, and the column 6 moves forward a distance of the micropore spacing. Repeat the above operation when dripping the next micropore. The user can adapt to different micropore plates by replacing the gear 36 and the rack 4, thereby improving the universality of the device. The device can automatically adjust the dripping position of the dropper after the user drips the sample, thereby improving the work efficiency of the inspector.

[0030] like Figure 2 and Figure 4 As shown, the sample loading assembly includes a pair of guide frames 20 arranged on the control ring 16, and a pair of guide frames 11 are symmetrically arranged on the fixed ring 13. Sliding blocks 10 are slidably clamped in the two guide frames 11, and the top of the sliding block 10 is connected to the clamping plate 9. The bottom ends of the two sliding blocks 10 are respectively clamped in the two guide frames 20.

[0031] like Figure 2 and Figure 4As shown, a control rod 12 is rotatably clamped on a retaining ring 22 connected to the outside of the control ring 16, a first end plate 23 is provided at the bottom end of the retaining ring 22, a second end plate 7 is provided at the bottom end of the fixing ring 13, an arc rod 24 provided on the first end plate 23 is inserted into a fixing sleeve 8 provided on the second end plate 7, and a second spring 17 is provided between the first end plate 23 and the second end plate 7.

[0032] like Figure 2 and Figure 4 As shown, a calibration ring 15 connected by a connecting frame 19 is provided at the bottom end of the fixed ring 13, and a radiation calibrator is provided on the calibration ring 15. The axis of the calibration ring 15 and the focal point of the radiation calibrator are both located on the same vertical line as the axis of the fixed ring 13.

[0033] The sample adding assembly can help inspectors simplify the dripping operation. When sample adding is required, first calibrate the initial position of the sample adding assembly through the calibration ring 15, then complete the placement of the dropper, and then move the control rod 12 connected to the outside of the control ring 16 through the clamping ring 22 to make the control ring 16 rotate at the bottom end of the fixed ring 13. Since a pair of guide frames 20 are provided on the control ring 16, the bottom ends of the two sliding blocks 10 are respectively clamped in the two guide frames 20. Therefore, during the rotation of the control ring 16, the sliding blocks 10 will be driven to move along the guide frames 11 on the fixed ring 13. At this time, the splints 9 connected to the top of the sliding blocks 10 approach each other and squeeze the rubber balloon at the top of the dropper. At the same time, the arc rod 24 connected to the bottom end of the clamping ring 22 through the first end plate 23 is gradually clamped into the fixed sleeve 8 provided on the second end plate 7, and the second spring 17 is compressed. When the control ring 16 rotates 90 degrees, the spacing between the splints 9 is reduced to the minimum value, and the dropper drips out the sample.

[0034] like Figure 1 and Figure 5 As shown, a fixed component is provided on the movable frame 3, and the fixed component includes a connecting arm 30 which is clamped on a rotating block on the movable frame 3, a fixed frame 31 is provided at one end of the connecting arm 30, a positioning cylinder 25 is slidably clamped in the fixed frame 31, a pulling plate 27 connected by a connecting rod 28 is provided on the inner wall of the positioning cylinder 25, one end of the inner wall of the positioning cylinder 25 is connected to the fixed frame 31 by a third spring 26, and a side plate 29 provided on the outer side of the positioning cylinder 25 is slidably clamped in the fixed frame 31.

[0035] The fixing component can fix the rack 4. When the user needs to change the specifications of the gear 36 and the rack 4, the pull plate 27 can be pulled outward. At this time, the pull plate 27 will compress the third spring 26 and make the connecting rod 28 slide along the fixed frame 31. When the fixed cylinder is disengaged from the movable frame 3, the connecting arm 30 can be rotated along the movable frame 3 to remove the rack 4, and the specifications of the rack 4 and the gear 36 can be changed synchronously.

[0036] like Figure 1 and Figure 6As shown, a distance adjustment component is provided on the base plate 1, and the distance adjustment component includes a screw rod 33 threaded on the base plate 1, one end of the screw rod 33 is coaxially connected to a rotating plate 34, one end of the screw rod 33 is rotatably clamped on one side of the moving frame 3, and one end of the base plate 1 is provided with a ruler 2 parallel to the axis of the screw rod 33.

[0037] The distance adjustment component can help the inspector to load samples on different columns of microwells. When the device completes loading samples on a column of microwells, the gear 36 is reset, and the screw 33 screwed on the bottom plate 1 is rotated through the rotating plate 34, so that the moving frame 3 slides along the bottom plate 1 until the loading component moves to the top of the microwells in the adjacent column.

[0038] The above is only an embodiment of the present invention, and the common knowledge such as the known specific technical solutions and / or characteristics in the solution is not described in detail here. It should be pointed out that for those skilled in the art, without departing from the technical solution of the present invention, several modifications and improvements can be made, which should also be regarded as the protection scope of the present invention, and these will not affect the effect of the implementation of the present invention and the practicality of the patent. The scope of protection required by this application shall be based on the content of its claims, and the specific implementation methods and other records in the specification can be used to interpret the content of the claims.

Claims

1. An immunoassay microplate sample loading auxiliary device, characterized in that: The invention comprises a bottom plate (1) and a moving frame (3), wherein the moving frame (3) is slidably mounted on a pair of guide plates (5) arranged on the top surface of the bottom plate (1), and a linkage component is arranged on the moving frame (3), wherein the linkage component comprises a column (6) slidably mounted on the moving frame (3), wherein one end of the column (6) is provided with a fixing ring (13), and the bottom end of the fixing ring (13) is rotatably mounted with a control ring (16), and a sample loading component is arranged on the control ring (16), and the control ring (16) is provided with a control ring (16). 16) is provided with a pressure block (18) at the bottom end, a sliding rod (35) is provided on the top end of the column (6), a wedge block (38) is provided at one end of the sliding rod (35), the inclined side of the wedge block (38) is in contact with one side of the pressure block (18), the wedge block (38) and the column (6) are connected by a first spring (39), a pair of connecting rods (40) are hinged at the free end of the sliding rod (35), and the bottom ends of the two connecting rods (40) are hinged together with a driving frame (41).

2. The immunoassay microplate sample loading auxiliary device according to claim 1, characterized in that: A connecting block (37) is rotatably mounted on the upright column (6), a gear (36) is mounted on the connecting block (37), a driving shaft (14) is eccentrically mounted on one side of the connecting block (37), the driving shaft (14) is mounted in the driving frame (41), a rack (4) is slidably mounted on the movable frame (3) via a T-shaped block (32), and the rack (4) is meshed with the gear (36).

3. The immunoassay microplate sample loading auxiliary device according to claim 2, characterized in that: The sample loading assembly comprises a pair of guide frames (20) arranged on the control ring (16), a pair of guide frames (11) symmetrically arranged on the fixed ring (13), a sliding block (10) being slidably mounted in each of the two guide frames (11), a clamping plate (9) being connected to the top of the sliding block (10), and the bottom ends of the two sliding blocks (10) being respectively mounted in the two guide frames (20).

4. The immunoassay microplate sample loading auxiliary device according to claim 3, characterized in that: A control rod (12) is rotatably mounted on a snap ring (22) connected to the outside of the control ring (16); a first end plate (23) is disposed at the bottom end of the snap ring (22); a second end plate (7) is disposed at the bottom end of the fixing ring (13); an arc-shaped rod (24) disposed on the first end plate (23) is inserted into a fixing sleeve (8) disposed on the second end plate (7); and a second spring (17) is disposed between the first end plate (23) and the second end plate (7).

5. The immunoassay microplate sample loading auxiliary device according to claim 4, characterized in that: A calibration ring (15) connected via a connecting frame (19) is arranged at the bottom end of the fixed ring (13); a radiation calibrator is arranged on the calibration ring (15); the axis of the calibration ring (15) and the focal point of the radiation calibrator are both located on the same vertical line as the axis of the fixed ring (13).

6. The immunoassay microplate sample loading auxiliary device according to claim 5, characterized in that: The movable frame (3) is provided with a fixing assembly, which comprises a connecting arm (30) which is fixedly mounted on a rotating block on the movable frame (3); a fixing frame (31) is provided at one end of the connecting arm (30); a positioning cylinder (25) is slidably mounted in the fixing frame (31); a pulling plate (27) connected via a connecting rod (28) is provided on the inner wall of the positioning cylinder (25); one end of the inner wall of the positioning cylinder (25) is connected to the fixing frame (31) via a third spring (26); and a side plate (29) arranged on the outer side of the positioning cylinder (25) is slidably mounted in the fixing frame (31).

7. The immunoassay microplate sample loading auxiliary device according to claim 6, characterized in that: The base plate (1) is provided with a pitch adjustment component, the pitch adjustment component comprising a screw rod (33) threadedly connected to the base plate (1), one end of the screw rod (33) being coaxially connected to a rotating plate (34), one end of the screw rod (33) being rotatably clamped on one side of the moving frame (3), and one end of the base plate (1) being provided with a ruler (2) parallel to the axis of the screw rod (33).

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