Cell culture device with filtering structure
By introducing filter structures and rotary culturers into the cell culture device, the problems of impurities and microbial contamination of nutrient solution are solved, ensuring the purity of the cell growth environment and the controllability of experimental conditions, achieving healthier cell growth and efficient experiments result.
Patent Information
- Application Number
- CN202422962322.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-03
- Publication Date
- 2025-01-03
- Estimated Expiration
- 2034-12-03
AI Technical Summary
Existing cell culture devices have failed to effectively solve the problems of impurities and microbial contamination in nutrient solution and precise control of supply, resulting in abnormal cell growth or death.
A cell culture device with a filter structure is designed, including a built-in filter cartridge and an internal filter mesh, which enables the filtration and precise dropping of nutrient solution by controlling the pump and dropper head, and simulates the microgravity environment through a rotary incubator, combined with The electric heating plate and display screen are for temperature control and real-time monitoring.
Effectively remove impurities and microorganisms in nutrient solution, ensure the purity of the cell growth environment, simulate the microgravity environment to promote cell growth, and improve culture density and experimental controllability and reliability.
Smart Images
Figure CN222294098U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of cell culture, in particular to a cell culture device with a filtering structure. Background Art
[0002] Cell culture technology is a method of taking living tissues or cells out of experimental animals and placing them in an in vitro environment (sterile, suitable temperature, and rich in nutrients) that simulates the living environment in the body to make them grow and develop. With the continuous development and improvement of cell culture technology, cell culture has gradually become a widely used technical method in biological and medical research and applications. Especially in the field of medicine, cell culture technology has developed rapidly and is used in many aspects such as drug screening, disease model establishment, and cell therapy.
[0003] For example, Chinese patent CN219526661U discloses a cell culture device, which may have breakthroughs in the stability of the culture environment, cell growth monitoring, and convenient replacement of culture dishes. Although the patent solves some existing problems, it does not pay enough attention to the filtration and precise control of the nutrient solution supply process, which is an indispensable part of cell culture. During the cell culture process, the purity and supply of the nutrient solution have a vital impact on the growth and differentiation of cells. If the nutrient solution contains impurities or microorganisms, or the supply amount cannot be accurately controlled, it may cause abnormal cell growth and even cause cell death. However, the patent may not provide a complete solution to this problem, resulting in the need to rely on additional equipment or operations to ensure the purity and supply of the nutrient solution in actual applications. For this reason, we propose a cell culture device with a filtration structure. Utility Model Content
[0004] 1. Technical issues to be resolved
[0005] In view of the deficiencies in the prior art, the utility model provides a cell culture device with a filtering structure to solve the above-mentioned problems.
[0006] (II) Technical solution
[0007] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: a cell culture device with a filtering structure, comprising a culture box, a partition is fixedly connected to one side of the inner wall of the culture box, a worm gear is movably connected to the bottom of the inner wall of the culture box, and the top output end of the worm gear passes through the top of the partition, the top output end of the worm gear is fixedly connected to a rotating disk, and a plurality of culture dishes are plugged into the top of the rotating disk, a worm is movably connected to one end of the inner wall of the partition, a forward and reverse motor is fixedly connected to one side of the bottom of the inner wall of the culture box, the output end of the forward and reverse motor is fixedly connected to the output end of one end of the worm, the worm and the worm wheel are threadedly connected, wherein the worm is driven to rotate by the forward and reverse motor, and the threaded connection structure of the worm and the worm wheel enables the worm wheel and the rotating disk fixedly connected thereto to rotate stably and accurately, thereby driving the culture dishes to rotate, simulating a microgravity environment, and promoting cell growth, and also includes:
[0008] A filtering structure is fixedly connected to the top output end of the incubator.
[0009] Preferably, the culture dishes are provided in twelve groups.
[0010] Preferably, a control box is fixedly connected to the top of the incubator, a filter cartridge is fixedly connected to one side of the top of the control box, and the bottom output end of the filter cartridge passes through the top of the inner wall of the incubator, an inner filter is fixedly connected to one end of the inner wall of the filter cartridge, and a plurality of filter holes are opened on the inner wall of one end of the inner filter.
[0011] Preferably, a control pump is fixedly connected to the bottom output end of the filter hole, a dropper head is fixedly connected to the bottom output end of the control pump, and the output end of the dropper head is located directly above the worm.
[0012] Preferably, an electric heating plate is fixedly connected to one side of the inner wall of the incubator, and two groups of electric heating plates are provided.
[0013] Preferably, a plurality of control buttons are fixedly connected to the outer wall at one end of the filter cartridge, and a display screen is fixedly connected to the outer wall at one end of the filter cartridge close to the control buttons.
[0014] Preferably, a plurality of anti-slip pads are fixedly connected to the bottom of the incubator, a light-transmitting glass door is movably connected to an output end at one end of the incubator, and a handle is fixedly connected to the outer wall at one end of the light-transmitting glass door.
[0015] (III) Beneficial effects
[0016] Compared with the prior art, the utility model provides a cell culture device with a filtration structure, which has the following beneficial effects:
[0017] 1. The cell culture device with a filtration structure can effectively remove impurities, particles and potential microbial contamination in the nutrient solution through the built-in filtration structure, ensure the purity of the cell culture environment, and provide a healthier and more stable growth condition for the cells.
[0018] 2. The cell culture device with a filtration structure generates centrifugal force by rotating the culture vessel to simulate a microgravity environment, which can change the growth behavior and differentiation pattern of cells. The microgravity environment helps the growth and proliferation of cells, increases the culture density, and promotes the interaction between cells.
[0019] 3. The cell culture device with a filtration structure and the rotating culture device provide a stable culture environment. By precisely controlling parameters such as the rotation speed, culture time and temperature, the consistency of experimental conditions can be ensured each time. This helps to enhance the controllability and repeatability of the experiment and improve the reliability and accuracy of the research results. BRIEF DESCRIPTION OF THE DRAWINGS
[0020] Figure 1 It is a schematic cross-sectional view of the structure of the utility model;
[0021] Figure 2 This is a schematic diagram of the internal structure of the utility model;
[0022] Figure 3 It is a schematic diagram of the structure of the utility model.
[0023] In the figure: 1. incubator; 2. partition; 3. worm gear; 4. rotating disk; 5. culture dish; 6. worm; 7. forward and reverse motor; 8. control box; 9. filter cartridge; 10. inner filter; 11. filter hole; 12. control pump; 13. dropper head; 14. electric heating plate; 15. control button; 16. display screen; 17. anti-slip mat; 18. light-transmitting glass door; 19. handle. DETAILED DESCRIPTION
[0024] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.
[0025] 1. Please refer to Figure 1-3A cell culture device with a filtering structure comprises a culture box 1, a partition 2 is fixedly connected to one side of the inner wall of the culture box 1, a worm gear 3 is movably connected to the bottom of the inner wall of the culture box 1, and the top output end of the worm gear 3 passes through the top of the partition 2, a rotating disk 4 is fixedly connected to the top output end of the worm gear 3, and a plurality of culture dishes 5 are plugged on the top of the rotating disk 4, a worm 6 is movably connected to one end of the inner wall of the partition 2, a forward and reverse motor 7 is fixedly connected to one side of the bottom of the inner wall of the culture box 1, the output end of the forward and reverse motor 7 is fixedly connected to the output end of one end of the worm 6, the worm 6 and the worm wheel 3 are threadedly connected, wherein the worm 6 is driven to rotate by the forward and reverse motor 7, and the threaded connection structure of the worm 6 and the worm wheel 3 enables the worm wheel 3 and the rotating disk 4 fixedly connected thereto to rotate stably and accurately, thereby driving the culture dishes 5 to rotate, simulating a microgravity environment, and promoting cell growth, and also comprises:
[0026] The filtering structure is fixedly connected to the top output end of the incubator 1 .
[0027] Furthermore, the culture dishes 5 are provided in twelve groups.
[0028] Furthermore, a control box 8 is fixedly connected to the top of the incubator 1, a filter cartridge 9 is fixedly connected to one side of the top of the control box 8, and the bottom output end of the filter cartridge 9 passes through the top of the inner wall of the incubator 1, an inner filter screen 10 is fixedly connected to one end of the inner wall of the filter cartridge 9, and a plurality of filter holes 11 are opened on the inner wall of one end of the inner filter screen 10.
[0029] Furthermore, a control pump 12 is fixedly connected to the bottom output end of the filter hole 11 , a dropper head 13 is fixedly connected to the bottom output end of the control pump 12 , and the output end of the dropper head 13 is located directly above the worm 6 .
[0030] Furthermore, an electric heating plate 14 is fixedly connected to one side of the inner wall of the incubator 1 , and two groups of the electric heating plates 14 are provided.
[0031] Furthermore, a plurality of control buttons 15 are fixedly connected to the outer wall of one end of the filter cartridge 9 , and a display screen 16 is fixedly connected to the outer wall of one end of the filter cartridge 9 close to the control buttons 15 .
[0032] Furthermore, a plurality of anti-skid pads 17 are fixedly connected to the bottom of the incubator 1 , a light-transmitting glass door 18 is movably connected to an output end of one end of the incubator 1 , and a handle 19 is fixedly connected to the outer wall of one end of the light-transmitting glass door 18 .
[0033] Working principle: First, the electric heating plate 14 is started by the control button 15 on the control box 8 to preheat the inside of the incubator 1 to ensure the appropriate temperature required for cell culture. The electric heating plate 14 is provided with two groups, which can maintain the temperature in the incubator more evenly. The culture dish 5 containing cells is plugged into the rotating disk 4. There are twelve groups of culture dishes in total, and multiple or large-scale cell cultures can be carried out at the same time. Start the forward and reverse motor 7, and its output end drives the worm 6 to rotate. The worm 6 is threadedly connected to the worm wheel 3, so the rotation of the worm 6 drives the worm wheel 3 to rotate at the bottom of the incubator 1, and then drives the rotating disk 4 and the culture dish 5 to rotate. This rotation helps the cells to be evenly distributed in the culture solution and improves the culture effect. During the cell culture process, it may be necessary to add nutrients to the culture solution or perform other treatments. At this time, the filtration and precise dripping of the nutrient solution can be achieved through the coordinated work of the filter cartridge 9 and the control pump 12. Specifically, the nutrient solution is first filtered through the inner filter 10 in the filter cartridge 9 to remove impurities and particles, and then enters the control pump 12 through the filter hole 11. The control pump 12 accurately drips the filtered nutrient solution into the rotating culture dish 5 through the dropper head 13 according to the preset program or manual control to ensure that the cells obtain sufficient nutrition. During the culture process, the display screen 16 at one end of the filter cylinder 9 can monitor the temperature, humidity and other parameters in the culture box in real time, as well as the status of the filtering and dripping process. At the same time, the control button 15 is used to adjust various parameters, such as the temperature of the electric heating plate, the dripping speed of the control pump, etc., to achieve precise cell culture control. The bottom of the culture box 1 is provided with an anti-slip mat 17 to ensure the stability of the device during use. The design of the light-transmitting glass door 18 and the handle 19 allows the user to easily observe the cell growth in the culture box and facilitates the operation of taking and placing the culture dish.
[0034] Although the embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.
Claims
1. A cell culture device with a filtration structure, comprising a culture box (1), characterized in that: A partition (2) is fixedly connected to one side of the inner wall of the incubator (1); a worm gear (3) is movably connected to the bottom of the inner wall of the incubator (1); the top output end of the worm gear (3) passes through the top of the partition (2); the top output end of the worm gear (3) is fixedly connected to a rotating disk (4); a plurality of culture dishes (5) are plugged into the top of the rotating disk (4); a worm (6) is movably connected to one end of the inner wall of the partition (2); a forward and reverse motor (7) is fixedly connected to one side of the bottom of the inner wall of the incubator (1); the output end of the forward and reverse motor (7) is fixedly connected to one end of the output end of the worm (6); the worm (6) and the worm gear (3) are threadedly connected, wherein the worm (6) is driven to rotate by the forward and reverse motor (7); the threaded connection structure of the worm gear (6) and the worm gear (3) enables the worm gear (3) and the rotating disk (4) fixedly connected thereto to rotate stably and accurately, thereby driving the culture dishes (5) to rotate, simulating a microgravity environment, and promoting cell growth, and is characterized in that: A filtering structure, wherein the filtering structure is fixedly connected to the top output end of the incubator (1).
2. A cell culture device with a filtration structure according to claim 1, characterized in that: The culture dishes (5) are provided in twelve groups.
3. The cell culture device with a filtration structure according to claim 1, characterized in that: A control box (8) is fixedly connected to the top of the incubator (1), a filter cartridge (9) is fixedly connected to one side of the top of the control box (8), and the bottom output end of the filter cartridge (9) passes through the top of the inner wall of the incubator (1), an inner filter screen (10) is fixedly connected to one end of the inner wall of the filter cartridge (9), and a plurality of filter holes (11) are provided on the inner wall of one end of the inner filter screen (10).
4. The cell culture device with a filtration structure according to claim 3, characterized in that: The bottom output end of the filter hole (11) is fixedly connected to a control pump (12), the bottom output end of the control pump (12) is fixedly connected to a dropper head (13), and the output end of the dropper head (13) is located directly above the worm (6).
5. The cell culture device with a filtration structure according to claim 1, characterized in that: An electric heating plate (14) is fixedly connected to one side of the inner wall of the incubator (1), and two groups of the electric heating plates (14) are provided.
6. The cell culture device with a filtration structure according to claim 3, characterized in that: A plurality of control buttons (15) are fixedly connected to the outer wall of one end of the filter cartridge (9), and a display screen (16) is fixedly connected to the outer wall of one end of the filter cartridge (9) close to the control buttons (15).
7. The cell culture device with a filtration structure according to claim 1, characterized in that: A plurality of anti-slip pads (17) are fixedly connected to the bottom of the incubator (1), a light-transmitting glass door (18) is movably connected to an output end of one end of the incubator (1), and a handle (19) is fixedly connected to the outer wall of one end of the light-transmitting glass door (18).