Nematode separation device for laboratory

Abstract

The utility model relates to the technical field of experimental equipment, and discloses a nematode separating device for a laboratory, which comprises a mesh screen consisting of a screen drum and a screen and a water receiving container separably mounted below the mesh screen, a water containing device capable of enabling water in the mesh screen to be in contact with soil is arranged at the lower end of the mesh screen, and the water containing device comprises a base and a movable plug. The base is fixedly installed at the bottom of the mesh screen, the base and the mesh screen are both made of shading materials, an annular rubber pad is arranged on the edge of the bottom of the base, the base is tightly attached to the inner wall of the water receiving container through the annular rubber pad, a water leakage hole is formed in the middle of the base, and the movable plug is movably installed in the water leakage hole. And a sealing ring is arranged between the movable plug and the bottom of the inner wall of the base. The utility model aims to solve the problems that in a test for separating nematodes by using a funnel method, the number of soil samples in a funnel is large, the moving distance of the nematodes is very long when the nematodes penetrate through soil to reach a water sample plane, and the number of the separated nematodes is small.

Description

Technical Field

[0001] The utility model belongs to the technical field of experimental equipment, and particularly relates to a nematode separation device for laboratory use. Background Art

[0002] In the soil separation test of tobacco root-knot nematodes, a large number of nematodes need to be separated. The common practices in this work are sieving and funnel methods. The sieving method is mainly used to separate various types of nematodes from the soil. The soil-water mixture needs to be sieved through a 20-mesh sieve, a 325-mesh sieve, and a 500-mesh sieve. This method can collect a large number of nematodes, but it cannot distinguish between dead worms, inactive nematodes and worm-type nematodes. The funnel method can separate the active worm-type nematodes required for the experiment, but in the nematode separation test using the funnel method, there are more soil samples in the funnel. When the nematodes pass through the soil to reach the water sample plane, the distance they move is very long, and the number of nematodes separated is small. In order to improve the efficiency of the test, we designed a laboratory nematode separation device. Utility Model Content

[0003] The utility model aims to solve the problem that in a nematode separation test using a funnel method, there are many soil samples in the funnel, the nematodes move a very long distance when passing through the soil to reach the water sample plane, and the number of nematodes separated is small.

[0004] In order to achieve the above technical objectives, the technical solutions adopted by this utility model are as follows:

[0005] A laboratory nematode separation device comprises a mesh screen consisting of a sieve cylinder and a mesh, and a water receiving container detachably mounted below the mesh screen, a water holding device being provided at the lower end of the mesh screen for allowing water in the mesh screen to contact soil, the water holding device comprising a base and a movable plug, the base being fixedly mounted on the bottom of the mesh screen, and both the base and the mesh screen being made of light-shielding material, an annular rubber pad being provided at the bottom edge of the base, the base being tightly fitted to the inner wall of the water receiving container through the annular rubber pad, a water leakage hole being provided in the middle of the base, the movable plug being movably mounted in the water leakage hole, a sealing ring being provided between the movable plug and the bottom of the inner wall of the base, a cover plate being movably mounted on the upper end of the mesh screen, the cover plate being made of a transparent material, a plurality of air holes communicating with the bottom being provided on the side of the cover plate, and an illuminating lamp being provided at the lower end of the cover plate.

[0006] The invention further defines that the water receiving container is composed of a water receiving funnel and a liquid storage cylinder, which are made of glass, and the water receiving funnel is fixedly mounted on the mouth of the liquid storage cylinder. Such a structural design makes the water receiving funnel and the liquid storage cylinder an integrated glass container, which is convenient for observation and improves the overall stability of the device.

[0007] It is further defined that the inner wall of the water receiving funnel is fixedly mounted with an arc-shaped support bar for supporting the mesh screen. Such a structural design facilitates positioning and supporting the mesh screen placed in the water receiving funnel through the arc-shaped support bar, so as to facilitate the placement of the mesh screen.

[0008] It is further defined that the base is fixedly connected to a water inlet pipe and a water outlet pipe, one end of the water outlet pipe extends into the mesh screen and is located above the screen, and the other end is provided with a filter terminal. The upper end of the water inlet pipe is provided with a magnetic sleeve, and the upper end of the magnetic sleeve is detachably connected to the water inlet funnel, and the lower end of the water inlet funnel is magnetically connected to the magnetic sleeve. With this structural design, the water inlet funnel is magnetically mounted to the water inlet pipe, which is convenient for installation and removal as needed.

[0009] Furthermore, the cover plate is fixedly mounted with a plurality of fixing rods at its lower end. When the cover plate is placed on the mesh screen, the lower ends of the fixing rods extend into the mesh screen. With this structural design, when the cover plate is rotated, the fixing rods can move the soil in the mesh screen, creating grooves in the soil. This allows light to penetrate the grooves, driving more nematodes downward.

[0010] The invention further defines that two positioning posts are fixedly mounted on the lower end of the base, and the upper end of the cover plate is provided with grooves that mate with the ends of the movable plug and the positioning posts. The mesh screen and cover plate are arranged in a group, and multiple sets of mesh screens and cover plates can be installed on the water receiving container. This structural design, in which the positioning posts mate with the grooves, allows the rotation of the lower cover plate adjacent to the mesh screen, thereby improving nematode separation efficiency. Furthermore, by stacking multiple sets of mesh screens and cover plates, multiple groups of soil nematodes can be separated simultaneously, saving space and increasing convenience.

[0011] The utility model adopting the above technical solution has the following advantages:

[0012] The utility model combines the mesh screen and the Baermann funnel method in the nematode separation method, and does not require light-shielding treatment like traditional separation tools. The lower water layer is surrounded by the mesh screen and the light-shielding material of the base, and is itself under light-shielding conditions. In addition, a cover plate and an illuminating lamp are arranged above the mesh screen, and the light-avoiding property of the nematodes is utilized to make them wriggle into the water surface by themselves, thereby improving the nematode separation rate and being convenient to use. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] The present invention can be further described by way of non-limiting examples given in the accompanying drawings;

[0014] Figure 1 This is a schematic structural diagram of a laboratory nematode separation device according to the present invention;

[0015] Figure 2 This is a schematic structural diagram of a water receiving container in a laboratory nematode separation device of the present invention;

[0016] Figure 3 This is a structural diagram of the mesh screen and cover plate in a laboratory nematode separation device of the present invention;

[0017] Figure 4 This is a structural diagram of the mesh screen and cover plate in a laboratory nematode separation device according to the present invention from another angle;

[0018] Figure 5 This is a schematic cross-sectional view of the mesh screen in a laboratory nematode separation device of the present invention;

[0019] Figure 6 for Figure 5 Schematic diagram of the enlarged structure at point A in the middle.

[0020] The main component symbols are described as follows:

[0021] 1. Mesh screen;

[0022] 2. Water receiving container;

[0023] 21. Water receiving funnel; 211. Arc-shaped supporting bar;

[0024] 22. Liquid storage cylinder;

[0025] 3. Base; 31. Water inlet pipe; 32. Water outlet pipe; 33. Water inlet funnel; 34. Positioning column;

[0026] 4. Movable plug;

[0027] 5. Cover plate; 51. Lighting lamp; 52. Fixing rod; 53. Groove. DETAILED DESCRIPTION

[0028] The present invention will be described in detail below with reference to the accompanying drawings and specific embodiments. It should be noted that similar or identical parts in the drawings or in the specification are numbered the same. Implementations not shown or described in the drawings are forms known to those skilled in the art. In addition, directional terms such as "upper," "lower," "top," "bottom," "left," "right," "front," and "back" mentioned in the embodiments are merely references to the directions in the drawings and are not intended to limit the scope of protection of the present invention.

[0029] like Figures 1 to 6As shown, the utility model is a laboratory nematode separation device, comprising a mesh screen 1 composed of a sieve cylinder and a screen mesh and a water receiving container 2 detachably installed below the mesh screen 1, a water holding device is provided at the lower end of the mesh screen 1, which can make the water in the mesh screen 1 contact with the soil, the water holding device comprises a base 3 and a movable plug 4, the base 3 is fixedly mounted on the bottom of the mesh screen 1, and the base 3 and the mesh screen 1 are both made of light-shielding material, an annular rubber pad is provided at the bottom edge of the base 3, the base 3 is tightly fitted with the inner wall of the water receiving container 2 through the annular rubber pad, a water leakage hole is opened in the middle of the base 3, the movable plug 4 is movably installed in the water leakage hole, a sealing ring is provided between the movable plug 4 and the bottom of the inner wall of the base 3, a cover plate 5 is movably installed on the upper end of the mesh screen 1, the cover plate 5 is made of transparent material, a plurality of air holes connected to the bottom are opened on the side of the cover plate 5, and an illuminating lamp 51 is provided at the lower end of the cover plate 5.

[0030] The water receiving container 2 is composed of a water receiving funnel 21 and a liquid storage cylinder 22 , and the water receiving funnel 21 is fixedly installed at the cylinder mouth of the liquid storage cylinder 22 .

[0031] An arc-shaped support bar 211 for supporting the mesh screen 1 is fixedly installed on the inner wall of the water receiving funnel 21. The arc-shaped support bar 211 can produce a positioning effect on the base 3 to prevent the base 3 from tilting when placed, which affects the experiment.

[0032] A water inlet pipe 31 and a water outlet pipe 32 are fixedly connected to the base 3. One end of the water outlet pipe 32 extends into the mesh screen 1 and is located above the mesh. The other end is provided with a filter end to prevent turbid liquid containing mud from flowing out of the water outlet pipe 32, which affects the subsequent separation and selection efficiency of nematodes. A magnetic sleeve is provided at the upper end of the water inlet pipe 31, and a water inlet funnel 33 is detachably connected to the top of the magnetic sleeve. The lower end of the water inlet funnel 33 is magnetically connected to the magnetic sleeve. The magnetic attraction between the lower end of the water inlet funnel 33 and the magnetic sleeve makes it convenient to disassemble and assemble the water inlet funnel 33.

[0033] A plurality of fixing rods 52 are fixedly mounted on the lower end of the cover plate 5 . When the cover plate 5 is placed on the mesh screen 1 , the lower ends of the fixing rods 52 extend into the mesh screen 1 .

[0034] Two positioning columns 34 are fixedly installed at the lower end of the base 3, and a groove 53 is formed at the upper end of the cover plate 5 to match the end of the movable plug 4 and the end of the positioning column 34. The mesh screen 1 and the cover plate 5 are arranged in a group, and multiple groups of mesh screens 1 and cover plates 5 can be arranged on the water receiving container 2.

[0035] The method of using the utility model is as follows:

[0036] Before use, clean the entire separation device to make the mesh 1 moist. At the same time, check the tightness of the device. After filling with an appropriate amount of sterile water, check whether the bottom is tightly sealed and whether there is any leakage. If there is leakage, rotate the movable plug 4 so that the sealing ring is close to the bottom of the inner wall of the base 3, reseal and check again until it is completely sealed.

[0037] Place the mesh 1 in the water receiving container 2, with the arc-shaped support bar 211 supporting the base 3. The annular rubber pad at the lower edge of the base 3 fits tightly against the inner wall of the water receiving funnel 21. Place a layer of nematode filter paper on the mesh 1, evenly covering the entire structure. Spread the soil evenly on the mesh of the mesh 1, place the cover plate 5 on the mesh 1, and stack multiple groups of meshes 1 and cover plates 5. Subsequently, add RO water to the water inlet funnel 33 until water flows out of the outlet pipe 32. Then, turn on the lighting lamp 51 to provide light above the soil to drive away the nematodes in the soil.

[0038] After 24 hours, check whether there is water on the surface of the water outlet pipe 32. If not, add RO water to the water inlet pipe 31 until water flows out of the water outlet pipe 32 again. Then rotate the upper mesh screen 1 from bottom to top, so that the positioning column 34 inserted into the groove 53 on the base 3 drives the cover plate 5 below to rotate, so that the fixing rod 52 draws grooves on the soil, promoting light to penetrate into the grooves, thereby improving the effect of light-induced insect repellent.

[0039] After 48 hours, take out the soil sample and filter paper, take out the water inlet funnel 33, pull out the movable plug 4, and put the water at the bottom of the base 3 into the water receiving funnel 21 and liquid storage cylinder 22 below. Use a washing bottle to clean the mesh 1, and the cleaned water will flow into the water receiving container 2 below.

[0040] The above describes in detail the laboratory nematode separation device provided by the present invention. The description of the specific embodiments is intended only to facilitate understanding of the method and core concept of the present invention. It should be noted that those skilled in the art may make various improvements and modifications to the present invention without departing from the principles of the present invention, and such improvements and modifications are also within the scope of protection of the claims of the present invention.

Claims

1. A laboratory nematode separation device, comprising a screen (1) consisting of a screen cylinder and a screen mesh, and a water receiving container (2) detachably mounted below the screen (1), characterized in that: The lower end of the mesh screen (1) is provided with a water holding device that allows the water in the mesh screen (1) to contact the soil. The water holding device comprises a base (3) and a movable plug (4) on the outer side. The base (3) is fixedly mounted on the bottom of the mesh screen (1), and the base (3) and the mesh screen (1) are both made of light-shielding material. An annular rubber pad is provided on the bottom edge of the base (3). The base (3) is tightly fitted with the inner wall of the water receiving container (2) through the annular rubber pad. A water leakage hole is provided in the middle of the base (3). The movable plug (4) is movably mounted in the water leakage hole. A sealing ring is provided between the movable plug (4) and the bottom of the inner wall of the base (3). A cover plate (5) is movably mounted on the upper end of the mesh screen (1). The cover plate (5) is made of a transparent material. A plurality of air holes connected to the bottom are provided on the side of the cover plate (5). An illuminating lamp (51) is provided at the lower end of the cover plate (5).

2. A laboratory nematode separation device according to claim 1, characterized in that: The water receiving container (2) is composed of a water receiving funnel (21) and a liquid storage cylinder (22), and the water receiving funnel (21) is fixedly mounted on the mouth of the liquid storage cylinder (22).

3. A laboratory nematode separation device according to claim 2, characterized in that: An arc-shaped supporting bar (211) for supporting the mesh screen (1) is fixedly mounted on the inner wall of the water receiving funnel (21).

4. A laboratory nematode separation device according to claim 1, characterized in that: A water inlet pipe (31) and a water outlet pipe (32) are fixedly connected to the base (3); one end of the water outlet pipe (32) extends into the mesh screen (1) and is located above the mesh screen, and the other end is provided with a filter end; a magnetic suction sleeve is provided at the upper end of the water inlet pipe (31); a water inlet funnel (33) is detachably connected above the magnetic suction sleeve; and the lower end of the water inlet funnel (33) is magnetically connected to the magnetic suction sleeve.

5. A laboratory nematode separation device according to claim 1, characterized in that: A plurality of fixing rods (52) are fixedly mounted on the lower end of the cover plate (5); when the cover plate (5) is placed on the mesh screen (1), the lower ends of the plurality of fixing rods (52) extend into the mesh screen (1).

6. A laboratory nematode separation device according to claim 1, characterized in that: Two positioning columns (34) are fixedly mounted on the lower end of the base (3); a groove (53) adapted to the ends of the movable plug (4) and the positioning columns (34) is provided on the upper end of the cover plate (5); the mesh screen (1) and the cover plate (5) are arranged in groups; and a plurality of groups of mesh screens (1) and cover plates (5) can be arranged on the water receiving container (2).

Images