High-temperature tissue culture device for radix pseudostellariae

Abstract

The utility model relates to the technical field of tissue culture of radix pseudostellariae, and discloses a high-temperature tissue culture device for radix pseudostellariae, which comprises a culture box, support rods are correspondingly arranged on two sides in the culture box, the support rods are arranged in two layers up and down, a culture plate is connected onto the support rods in a sliding manner, a sliding chute matched with the culture plate is arranged on the culture box, and the culture plate is arranged in the sliding chute. The culture plate is provided with at least one culture groove, a culture medium is arranged in the culture groove, clamping plates which are correspondingly arranged on the two sides are arranged in the culture groove, cavities are formed in the positions, located on the two sides of the culture groove, of the culture plate, L-shaped insertion columns are movably inserted into the cavities, one ends of the L-shaped insertion columns penetrate through the cavities to be fixedly connected with the clamping plates, and the other ends of the L-shaped insertion columns penetrate through the cavities to be fixedly connected with the clamping plates. Compared with the prior art, the culture plate has the advantages that culture media can be automatically fixed after the culture plate is taken out, and meanwhile, all the culture media can be conveniently fixed in a contact manner, so that transplanting is facilitated.

Description

Technical Field

[0001] The utility model relates to the technical field of Pseudostellariae Pseudostellariae tissue culture, in particular to a Pseudostellariae ... high-temperature tissue culture device. Background Art

[0002] Tissue culture refers to the process of culturing and propagating cells or tissues in vitro. Through tissue culture technology, a large number of cells or tissues can be cultured by utilizing the characteristics of cell division and growth. Tissue culture is widely used in fields such as botany, medicine and biotechnology. It can be used to study the basic biology, biomedical research, plant propagation and cultivation, as well as to achieve applications such as tissue engineering and regenerative medicine.

[0003] When culturing Pseudostellaria heterophylla, first select tissue culture materials, such as Pseudostellaria heterophylla seeds or seedlings, then disinfect them, then place them in culture medium, and then put them on the culture rack in the incubator. When the Pseudostellaria heterophylla grows to a certain extent, it can be transplanted and the seedlings can be transplanted into suitable soil.

[0004] In the prior art, most of the culture racks in the tissue culture box are removable, so that it is convenient to transplant the Pseudostellaria baicalensis seedlings on the culture racks. However, during the transplanting process, the culture racks will shake, causing the Pseudostellaria baicalensis seedlings to shake and easily damage them. Using a fixing mechanism to fix the culture medium can prevent the Pseudostellaria baicalensis seedlings from being damaged, but it needs to be unfixed and taken out one by one, which is too cumbersome. Utility Model Content

[0005] 1. Technical issues to be resolved

[0006] The technical problem to be solved by the utility model is that the Pseudostellaria heterophylla seedlings need to be unfixed and taken out one by one during the transplanting process, which is too cumbersome.

[0007] (II) Technical solution

[0008] In order to solve the above technical problems, the technical solution provided by the utility model is: a high-temperature tissue culture device for Panax pseudoginseng, including an incubator, the incubator is provided with support rods arranged correspondingly on both sides, the support rods are arranged in two layers up and down, a culture plate is slidably connected to the support rods, the incubator is provided with a slide groove cooperating with the culture plate, the culture plate is provided with at least one culture groove, the culture groove is provided with a culture medium, the culture groove is provided with splints arranged correspondingly on both sides, cavities are provided on the culture plate on both sides of the culture groove, an L-shaped column is movably inserted in the cavity, one end of the L-shaped column passes through the cavity and is fixedly connected to the splint, a slot passing through the cavity is provided on the culture plate, a connecting column is movably inserted in the slot, the other end of the L-shaped column is fixedly connected to the connecting column, and a reset mechanism for driving the connecting column to move is provided in the cavity on one side.

[0009] As an improvement, the reset mechanism includes a limiting column on the connecting column, the limiting column is located in the cavity, and a spring sleeved on the connecting column is fixedly connected between the limiting column and the inner wall of the cavity.

[0010] As an improvement, one end of the connecting column is fixedly connected to a connecting plate, and one end of the connecting plate is an arc-shaped surface.

[0011] As an improvement, the incubator is provided with an observation slot and a display screen.

[0012] As an improvement, a heating component is embedded in the inner wall of the incubator, and a temperature sensor is fixedly connected inside the incubator.

[0013] As an improvement, both sides of the culture plate are fixedly connected with fixing columns, one end of the fixing columns is rotatably connected with a clamping plate, and the connecting plate is provided with a through hole that cooperates with the clamping plate.

[0014] (III) Beneficial effects

[0015] The advantages of the utility model compared with the prior art are:

[0016] When transplanting is needed, the culture plate is taken out, and the spring generates elastic force to drive the connecting column to move, thereby driving the L-shaped plug column in the cavity to move, thereby driving the splint to move, and fixing the culture medium, thereby automatically fixing the culture medium. When transplanting, the card plate is rotated to fix the connecting plate, so that the ginseng seedlings can be easily taken out, and there is no need to release the fixation one by one to take out the ginseng seedlings. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 The utility model is a stereoscopic diagram of a high-temperature tissue culture device for Pseudostellaria heterophylla.

[0018] Figure 2 The utility model discloses an explosion diagram of a high-temperature tissue culture device for Pseudostellaria heterophylla.

[0019] Figure 3 The utility model is a cross-section of a high temperature tissue culture device for Radix Pseudostellariae Figure 1 .

[0020] Figure 4 The utility model is a cross-section of a high temperature tissue culture device for Radix Pseudostellariae Figure 2 .

[0021] Figure 5 It is an enlarged view of position A of a high-temperature tissue culture device for Pseudostellaria heterophylla of the utility model.

[0022] As shown in the figure: 1. Incubator; 2. Support rod; 3. Culture plate; 4. Slide; 5. Culture tank; 6. Culture medium; 7. Clamp; 8. Cavity; 9. L-shaped column; 10. Slot; 11. Connecting column; 12. Limiting column; 13. Spring; 14. Connecting plate; 15. Observation slot; 16. Display screen; 17. Temperature sensor; 18. Fixed column; 19. Card; 20. Through hole. DETAILED DESCRIPTION

[0023] The technical solutions in the embodiments of the utility model will be clearly and completely described below in conjunction with the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, rather than all of the embodiments; based on the embodiments of the utility model, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the utility model.

[0024] like Figure 1 to Figure 2 As shown, a high-temperature tissue culture device for Pseudostellaria heterophylla includes an incubator 1, wherein the incubator 1 is provided with an observation slot 15, wherein the inside of the observation slot 15 is made of glass, wherein the incubator 1 is provided with a display screen 16, wherein a heating assembly is embedded in the inner wall of the incubator 1, wherein a temperature sensor 17 is fixedly connected to the incubator 1, wherein the incubator 1 is provided with support rods 2 correspondingly arranged on both sides, wherein the support rods 2 are arranged in two layers up and down, wherein a culture plate 3 is slidably connected to the support rods 2, wherein the incubator 1 is provided with a slide slot 4 cooperating with the culture plate 3, wherein the culture plate 3 is provided with at least one culture slot 5, wherein a culture medium 6 is provided in the culture slot 5, wherein the growth condition of Pseudostellaria heterophylla is observed through the observation slot 15, wherein the temperature detected by the temperature sensor 17 is displayed through the display screen 16, wherein the culture plate 3 is supported by the support rod 2, wherein the culture plate 3 is limited by the slide slot 4, wherein the culture slot 5 is used to place the culture medium 6.

[0025] like Figures 2 to 5As shown, the culture tank 5 is provided with a splint 7 arranged correspondingly on both sides, the culture plate 3 is provided with a cavity 8 on both sides of the culture tank 5, an L-shaped plug post 9 is movably inserted in the cavity 8, one end of the L-shaped plug post 9 passes through the cavity 8 and is fixedly connected to the splint 7, the culture plate 3 is provided with a slot 10 passing through the cavity 8, a connecting column 11 is movably inserted in the slot 10, the other end of the L-shaped plug post 9 is fixedly connected to the connecting column 11, a limiting column 12 is fixedly connected to the connecting column 11, the limiting column 12 is located in the cavity 8, and the limiting column 12 is fixedly connected to the cavity 8. A spring 13 sleeved on a connecting column 11 is fixedly connected between the walls, a connecting column 11 is fixedly connected to a connecting plate 14 at one end, one end of the connecting plate 14 is an arc-shaped surface, both sides of the culture plate 3 are fixedly connected to fixing columns 18, one end of the fixing column 18 is rotatably connected to a clamping plate 19, a through hole 20 is provided on the connecting plate 14 for cooperating with the clamping plate 19, the culture medium 6 is fixed by the clamping plate 7, the clamping plate 7 is controlled by the connecting column 11, the L-shaped plug column 9, the spring 13, and the connecting plate 14, and the connecting plate 14 is limited by the clamping plate 19, the fixing column 18, and the through hole 20.

[0026] In specific use, when it is necessary to culture the Pseudostellaria heterophylla, the seeds are placed in the culture medium 6. At this time, the connecting plate 14 is against the slide groove 4, so that the spring 13 is in a compressed state, and the splint 7 does not contact the culture medium 6, which is convenient for placing the culture medium 6 into the culture groove 5, and then the box door is closed for cultivation. When the cultivation is completed, the culture plate 3 is taken out, and when the connecting plate 14 is not in contact with the inner wall of the slide groove 4, the two springs 13 respectively generate thrust on the connecting columns 11 located on both sides of the placement groove, thereby pushing the L-shaped plug column 9 on the connecting column 11, and the L-shaped plug column 9 pushes the splint 7, and the two splints 7 are close to each other, thereby fixing the culture medium 6. During the transplanting process, The culture medium 6 will shake. When it reaches the place for transplanting, press the connecting plate 14 on one side, the spring 13 is compressed and deformed, and the connecting plate 14 drives the connecting column 11 to move, so that the clamp 7 is no longer in contact with the culture medium 6, and then the card plate 19 at one end of the fixed column 18 passes through the through hole 20 on the connecting plate 14. When it is completely passed through, rotate the card plate 19 to make it balanced with the culture plate 3. At this time, the spring 13 generates elastic force to make the connecting plate 14 and the card plate 19 fit firmly. According to the above operation, squeeze the connecting plate 14 on the other side, so that the culture medium 6 in the culture tank 5 can be fixed and released, which is convenient for transplanting, and then the culture plate 3 after transplanting is placed in the incubator 1.

[0027] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0028] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

[0029] The above description of the utility model and its implementation methods is not restrictive. The drawings show only one implementation method of the utility model, and the actual structure is not limited thereto. In short, if ordinary technicians in this field are inspired by it and design structural methods and embodiments similar to the technical solution without creativity without departing from the purpose of the invention of the utility model, they should all fall within the protection scope of the utility model.

Claims

1. A high temperature tissue culture device for Pseudostellaria heterophylla, comprising an incubator (1), characterized in that: The incubator (1) is provided with support rods (2) arranged correspondingly on both sides, the support rods (2) are arranged in two layers, a culture plate (3) is slidably connected to the support rods (2), the incubator (1) is provided with a slide groove (4) matched with the culture plate (3), the culture plate (3) is provided with at least one culture groove (5), and a culture medium (6) is provided in the culture groove (5); The culture tank (5) is provided with clamping plates (7) arranged correspondingly on both sides, and the culture plate (3) is provided with cavities (8) on both sides of the culture tank (5), and an L-shaped plug post (9) is movably inserted in the cavity (8), and one end of the L-shaped plug post (9) passes through the cavity (8) and is fixedly connected to the clamping plate (7); The culture plate (3) is provided with a slot (10) penetrating the cavity (8), a connecting column (11) is movably inserted into the slot (10), the other end of the L-shaped plug column (9) is fixedly connected to the connecting column (11), and a reset mechanism for driving the connecting column (11) to move is provided in the cavity (8) on one side.

2. A high temperature tissue culture device for Pseudostellaria heterophylla according to claim 1, characterized in that: The reset mechanism comprises a limit column (12) on the connecting column (11), the limit column (12) is located in the cavity (8), and a spring (13) sleeved on the connecting column (11) is fixedly connected between the limit column (12) and the inner wall of the cavity (8).

3. A high temperature tissue culture device for Pseudostellaria heterophylla according to claim 1, characterized in that: One end of the connecting column (11) is fixedly connected to a connecting plate (14), and one end of the connecting plate (14) is an arc-shaped surface.

4. The high temperature tissue culture device for Pseudostellaria heterophylla according to claim 1, characterized in that: The incubator (1) is provided with an observation slot (15), and the incubator (1) is provided with a display screen (16).

5. The high temperature tissue culture device for Pseudostellaria heterophylla according to claim 1, characterized in that: A heating component is embedded in the inner wall of the incubator (1), and a temperature sensor (17) is fixedly connected inside the incubator (1).

6. The high temperature tissue culture device for Pseudostellaria heterophylla according to claim 3, characterized in that: Both sides of the culture plate (3) are fixedly connected with fixing columns (18), one end of the fixing column (18) is rotatably connected with a clamping plate (19), and the connecting plate (14) is provided with a through hole (20) that cooperates with the clamping plate (19).

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