Trace reagent sample adding and containing bottle

Abstract

A trace reagent sample adding and containing bottle relates to the field of medical test instruments and comprises a bottle body, a bottle opening, a vent hole and a sealing mechanism, and a containing cavity used for containing reagents is formed in the bottle body; the bottle opening is formed in the top of the bottle body and can discharge reagents outwards; the vent holes are uniformly distributed at the bottom of the bottle body, so that the accommodating cavity is communicated with the outside to accelerate reagent discharge; the sealing mechanism is rotatably arranged at the bottom of the bottle body, the sealing mechanism is arranged at the bottom of the bottle body to seal the vent hole, the sealing mechanism comprises a first sealing plate, a second sealing plate and a sealing column, and the first sealing plate is rotatably connected with the second sealing plate; the sealing columns are respectively formed on the first sealing plate and the second sealing plate and are in one-to-one correspondence with the vent holes, and the sealing columns can seal the vent holes in stages along with the rotation of the first sealing plate and the second sealing plate, so that the reagents can be uniformly and normally discharged when the storage amounts of the reagents in the reagent bottles are different; the condition that the reagent is difficult to extrude or is strenuous to extrude when the reagent in the bottle body is too little is avoided, and the bottle has very high practicability.

Description

Technical Field

[0001] The utility model belongs to the field of medical experimental instruments, in particular to a trace reagent adding and containing bottle. Background Art

[0002] Existing immunohistochemistry reagents are divided into ready-to-use and concentrated types, mostly made of glass or plastic, with a volume of 1-6ml; some are large-capacity and are mostly used for fully automatic instrument detection, but only a few antibodies in a few pathology departments can detect a large number of reagents at a time. Most departments still need to test multiple antibodies together and manually add the primary antibody. Due to the high requirements for antibody production, the cost of the reagent is more expensive than other reagents in the pathology department; and in clinical external examinations, there are many kinds of detection antibody reagents needed to determine the prognosis or source of tumors, or for differential diagnosis of tumors. Sometimes, only one case appears in a year or even several years, and a certain reagent is needed. Therefore, it is inevitable that the reagent will expire and be wasted. Therefore, the reagents are mostly stored in small volumes to reduce the possible waste to the lowest level.

[0003] Pathological immunohistochemistry is currently an important and most widely used test item in the pathology department. Currently, most of the primary antibody reagents on the market are contained in single-opening plastic bottles with polyethylene resin as the main component, and the capacity is mostly 1.0-6ml. Because the plastic bottle body has a certain hardness, it can ensure the safety of the reagent. In immunohistochemistry testing, the amount of primary antibody required for each test sample is about 50-100 microliters (1-2 drops). The pathology technician needs to squeeze the bottle body with the index finger and thumb to drip the reagent on the tissue sample. This cyclic operation aggravates the strain of the technician's hand muscles. Secondly, when the reagent is added to a small amount, it is not only difficult to manually pressurize and squeeze out the reagent, but also the air quickly enters the bottle through the bottle mouth after relaxation and mixes with the reagent to form foam. When the antibody with foam is squeezed out again, it cannot effectively cover the tissue. Therefore, the last small amount of antibody is mostly discarded, resulting in waste, which needs to be improved. Utility Model Content

[0004] The utility model aims to overcome the shortcomings of the prior art and provide a micro-reagent sample adding and containing bottle.

[0005] The utility model adopts the following technical solutions:

[0006] A trace reagent loading and containing bottle comprises a bottle body, a bottle mouth, an air vent and a sealing mechanism, wherein a containing cavity for containing reagents is formed inside the bottle body; the bottle mouth is formed at the top of the bottle body to discharge the reagents outwardly; a plurality of air vents are arranged, evenly distributed at the bottom of the bottle body to connect the containing cavity with the outside to accelerate the discharge of the reagents; the sealing mechanism is rotatably arranged at the bottom of the bottle body, the sealing mechanism is arranged at the bottom of the bottle body to seal the air vents, the sealing mechanism comprises a first sealing plate, a second sealing plate and a sealing column, one end of the first sealing plate is rotatably connected to the bottle body; the second sealing plate is rotatably connected to the other end of the first sealing plate; a plurality of sealing columns are arranged, which are respectively formed on the first sealing plate and the second sealing plate and correspond to the air vents one by one, and the sealing columns can seal the air vents in stages as the first sealing plate and the second sealing plate rotate.

[0007] Preferably, an outer edge of the bottom of the bottle body is protruded outward to form an annular boss, and a first groove is recessed inward to form the annular boss, a rotating shaft is arranged in the first groove, a first protrusion matched with the first groove is formed on the first sealing plate, an axial hole opposite to the rotating shaft is formed on the first protrusion, and the first sealing plate rotates around the bottle body through the cooperation of the axial hole and the rotating shaft.

[0008] Preferably, it also includes a fixing mechanism, which includes a second groove, a second protrusion, a fixing hole, a positioning hole and a fixing piece, the second groove is formed on the annular boss and is opposite to the first groove; the second protrusion is formed on the second sealing plate and can be adapted to the second groove; the fixing hole extends outward from both sides of the second groove to be connected to the outside; the positioning hole is formed on the second protrusion and is connected to the fixing hole; the fixing piece can pass through the fixing hole and the positioning hole in sequence to fix the second sealing plate on the bottle body.

[0009] Preferably, the fixing member is a steel wire which can pass through the fixing hole and the positioning hole in sequence and self-lock to limit the mutual movement of the second sealing plate and the annular boss.

[0010] Preferably, a plurality of hinges are provided between the first sealing plate and the second sealing plate, and the second sealing plate is rotatably connected to the first sealing plate via the hinges.

[0011] Preferably, a plurality of support blocks are protrudingly formed on the annular boss, the height of the support blocks is greater than the thickness of the hinge, and the bottle body can be placed on the table with the mouth of the reagent bottle facing upward through the support blocks.

[0012] Preferably, the sealing column is a rubber cylinder with a diameter larger than the vent hole, and the end of the sealing column is inclined inward and concavely formed with a chamfer to facilitate the matching of the sealing column and the vent hole.

[0013] It can be seen from the above description of the utility model that, compared with the prior art, the utility model has the following beneficial effects: when the reagent bottle is squeezed downward to discharge the reagent, gas can enter the bottle body through the vent hole to quickly reset the bottle body, thereby preventing air from entering the bottle through the bottle mouth due to squeezing and mixing with the reagent to form a large amount of foam. At the same time, it can also reduce the use of force and avoid fatigue and injury of the technician's fingers during the experiment. By rotating the second sealing plate and the first sealing plate, and the first sealing plate and the bottle body, the vent hole can be opened or sealed in stages to adjust the air intake of the vent hole. When the reagent stock in the reagent bottle is different, it can ensure that the reagent can be discharged evenly and normally, thereby avoiding the situation where the reagent is difficult to squeeze or squeezed laboriously when there is too little reagent in the bottle body. The utility model has high practicality.

[0014] The first sealing plate and the second sealing plate can be rotated by a simple structure, thereby improving the convenience of operating the reagent bottle;

[0015] The fixing mechanism can fix the sealing mechanism on the base to prevent the sealing mechanism from being separated from the vent hole when the reagent is transported or subjected to external force, causing leakage of the reagent;

[0016] After the steel wire passes through the fixing hole and the positioning hole in turn, the two ends are quickly wound to complete the fixation, and the steel wire is easy to take;

[0017] The height of the support block is greater than the height of the hinge, so that the reagent bottle can be placed with the support of the support block, avoiding the problem that the bottom of the bottle is uneven and difficult to place due to the setting of the hinge. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 It is a structural schematic diagram of the utility model;

[0019] Figure 2 The working state of the utility model is shown in FIG. Figure 1 ;

[0020] Figure 3 The working state of the utility model is shown in FIG. Figure 2 ;

[0021] Figure 4 It is a cross-sectional view of the utility model;

[0022] Figure 5 This is a cutaway view of the working state of the utility model Figure 1 ;

[0023] Figure 6 This is a cutaway view of the working state of the utility model Figure 2 ;

[0024] In the figure: 1-bottle body, 11-accommodating cavity, 12-annular boss, 13-first groove, 14-rotating shaft, 15-support block, 2-bottle mouth, 3-vent, 4-sealing mechanism, 41-first sealing plate, 411-first protrusion, 412 shaft hole, 42-second sealing plate, 43-sealing column, 44-hinge, 5-fixing mechanism, 51-second groove, 52-second protrusion, 53-fixing hole, 54-positioning hole, 55-fixing piece. DETAILED DESCRIPTION

[0025] The present invention is further described below through specific implementation methods.

[0026] Referring to Figures 1 to 2, a trace reagent sample adding bottle includes a bottle body 1, a bottle mouth 2, a vent hole 3, a sealing mechanism 4, and a fixing mechanism 5.

[0027] A receiving cavity 11 for receiving a reagent is formed inside the bottle body 1 , and the bottle body 1 is made of soft plastic that is easy to press.

[0028] The bottle mouth 2 is formed on the top of the bottle body 1 to discharge the reagent outward.

[0029] Two vent holes 3 are provided, symmetrically distributed on both sides of the bottom of the bottle body 1, so that the accommodating cavity 11 can be connected with the outside to speed up the discharge of the reagent.

[0030] The sealing mechanism 4 can be rotatably arranged at the bottom of the bottle body 1. The sealing mechanism 4 is arranged at the bottom of the bottle body 1 to seal the vent hole 3. The sealing mechanism 4 includes a first sealing plate 41, a second sealing plate 42 and a sealing column 43. The right side of the first sealing plate 41 is rotatably connected to the right side of the bottle body 1; the right side of the second sealing plate 42 is rotatably connected to the left side of the first sealing plate 41; there are two sealing columns 43, which are respectively formed on the first sealing plate 41 and the second sealing plate 42 and correspond to the two vent holes 3 one by one. The sealing column 43 can seal the vent holes 3 in stages as the first sealing plate 41 and the second sealing plate 42 rotate. The sealing column 43 is a rubber cylinder with a diameter larger than the vent hole 3. The end of the sealing column 43 is tilted inward and concavely formed with a circle of chamfers for easy cooperation between the sealing column 43 and the vent hole 3. When the amount of reagent is large, open the second sealing plate 42 and keep the first sealing plate 41 still to open a vent 3, and air quickly enters the bottom of the bottle. At this time, the reagent can quickly flow out to form droplets under the action of atmospheric pressure and drip onto the tissue. If it is necessary to stop dripping the reagent at this time, just reseal the second sealing plate 42 after the dripping. Without the effect of external force and atmospheric pressure, the reagent will take a long time to come out even if the bottle mouth 2 of the reagent bottle is facing downward, so there is enough time for operation. When the amount of reagent is small, open the first sealing plate 41 and the second sealing plate at the same time. The sealing plate 42 allows the two vents 3 to be completely connected to the atmosphere, and the maximum amount of air enters the bottle. Since there is no squeezing, there is no force greater than the atmospheric pressure. After the air enters the bottle, the reagent quickly flows downward to the bottle mouth 2. It only needs to be squeezed slightly or allowed to fall naturally without any force. There will be no residue of reagent in the bottle, which avoids the situation where the technician needs to squeeze hard when there are only 1-2 doses left in the prior art. It is also possible that the air is sucked back into the bottle from the bottle mouth 2 during squeezing and mixed with the reagent to form foam. In order to ensure the accuracy of the experimental results, the last small amount of reagent has to be abandoned. Specifically, the outer edge of the bottom of the bottle body 1 is formed with an annular boss 12 protruding outward, and the annular boss 12 is formed with a first groove 13 recessed inward, and a rotating shaft 14 is arranged in the first groove 13. The first sealing plate 41 is formed with a first protrusion 411 adapted to the first groove 13, and the first protrusion 411 is formed with an axial hole 412 opposite to the rotating shaft 14. The first sealing plate 41 rotates around the bottle body 1 through the cooperation of the axial hole 412 and the rotating shaft 14. The rotation of the first sealing plate 41 and the second sealing plate 42 is realized through a simple structure, thereby improving the convenience of operating the reagent bottle. Furthermore, a plurality of hinges 44 are arranged between the first sealing plate 41 and the second sealing plate 42, and the second sealing plate 42 is rotatably connected to the first sealing plate 41 through the hinges 44. Preferably, a plurality of support blocks 15 are formed on the annular boss 12, and the height of the support blocks 15 is greater than the thickness of the hinge 44. The bottle body 1 can place the reagent bottle mouth 2 upward on the table through the support blocks 15. The height of the support blocks 15 is greater than the height of the hinge 44, so that the reagent bottle can be placed with the support of the support blocks 15, avoiding the uneven bottom of the bottle body 1 being difficult to place due to the setting of the hinge 44.

[0031] The fixing mechanism 5 includes a second groove 51, a second protrusion 52, a fixing hole 53, a positioning hole 54 and a fixing member 55. The second groove 51 is formed on the annular boss 12 and is opposite to the first groove 13; the second protrusion 52 is formed on the second sealing plate 42 and can be adapted to the second groove 51; the fixing hole 53 extends outward from both sides of the second groove 51 to be connected to the outside; the positioning hole 54 is formed on the second protrusion 52 and is connected to the fixing hole 53; the fixing member 55 can pass through the fixing hole 53 and the positioning hole 54 in turn to fix the second sealing plate 42 on the bottle body 1, and the fixing mechanism 5 can fix the sealing mechanism 4 on the base to prevent the sealing mechanism 4 from being separated from the vent hole 3 when the reagent is transported and subjected to external force, resulting in leakage of the reagent. Specifically, the fixing member 55 is a steel wire that can pass through the fixing hole 53 and the positioning hole 54 in turn and self-lock to limit the mutual movement of the second sealing plate 42 and the annular boss 12. The structure is simple and durable, and the steel wire is easy to use and can be quickly replenished if lost.

[0032] When in use, first check the remaining reagent in the bottle body 1. If there is a lot of remaining reagent, after tilting the bottle body 1, remove the fixing piece 55 to separate the second protrusion 52 from the second groove 51, drive the second sealing plate 42 to rotate around the first sealing plate 41 (the first sealing plate 41 does not move), so that the sealing column 43 on the second sealing plate 42 is separated from the corresponding vent 3, and air quickly enters the bottom of the bottle. At this time, the fingers hardly need to squeeze, and the reagent can flow out quickly under the action of atmospheric pressure to form droplets and drip on the tissue. If it is necessary to stop dripping the reagent at this time, just reseal the second sealing plate 42 after the dripping is completed and fix it with the fixing piece 55. Without the action of external force and atmospheric pressure, even if the bottle mouth 2 of the reagent bottle is facing downward, it will take a long time for the reagent to come out, so there is enough time for operation. If the remaining reagent is small, after the bottle body 1 is tilted, the fixing member 55 is removed to separate the second protrusion 52 from the second groove 51, and the first sealing plate 41 is rotated around the rotating shaft 14 to open all the vents 3. After the air enters the bottle, the reagent quickly flows downward to the bottle mouth 2. It only needs to be squeezed slightly or let it fall naturally without force, and there will be no residual reagent in the bottle. The progressive ventilation can control the reagent flow rate in a variable phase, which improves the fatigue and injury of the fingers of the pathology technician during the experiment, and there will be no residual reagent in the bottle, avoiding the waste of the last amount of reagent each time, which has high practicality.

[0033] The above description is only a preferred embodiment of the present utility model, and therefore cannot be used to limit the scope of implementation of the present utility model. That is, equivalent changes and modifications made according to the scope of the patent application of the present utility model and the contents of the specification should still fall within the scope covered by the patent of the present utility model.

Claims

1. A micro-reagent sample-adding bottle, characterized in that: It includes a bottle body, a bottle mouth, an air vent and a sealing mechanism, wherein a holding cavity for holding a reagent is formed inside the bottle body; the bottle mouth is formed at the top of the bottle body to discharge the reagent outward; a plurality of air vents are arranged, which are evenly distributed at the bottom of the bottle body to connect the holding cavity with the outside to accelerate the discharge of the reagent; the sealing mechanism is rotatably arranged at the bottom of the bottle body, and the sealing mechanism is arranged at the bottom of the bottle body to seal the air vent, and the sealing mechanism includes a first sealing plate, a second sealing plate and a sealing column, one end of the first sealing plate is rotatably connected to the bottle body; the second sealing plate is rotatably connected to the other end of the first sealing plate; a plurality of sealing columns are arranged, which are respectively formed on the first sealing plate and the second sealing plate and correspond to the air vents one by one, and the sealing columns can seal the air vents in stages as the first sealing plate and the second sealing plate rotate.

2. A trace reagent sample loading bottle according to claim 1, characterized in that: The outer edge of the bottom of the bottle body is protruded outward to form an annular boss, and the annular boss is recessed inward to form a first groove, a rotating shaft is arranged in the first groove, a first convex block matched with the first groove is formed on the first sealing plate, an axial hole opposite to the rotating shaft is formed on the first convex block, and the first sealing plate rotates around the bottle body through the cooperation of the axial hole and the rotating shaft.

3. A trace reagent sample loading bottle according to claim 2, characterized in that: It also includes a fixing mechanism, which includes a second groove, a second protrusion, a fixing hole, a positioning hole and a fixing piece, wherein the second groove is formed on the annular boss and is opposite to the first groove; the second protrusion is formed on the second sealing plate and can be adapted to the second groove; the fixing hole extends outward from both sides of the second groove to be connected to the outside; the positioning hole is formed on the second protrusion and is connected to the fixing hole; the fixing piece can pass through the fixing hole and the positioning hole in sequence to fix the second sealing plate on the bottle body.

4. A trace reagent sample loading bottle according to claim 3, characterized in that: The fixing member is a steel wire which can pass through the fixing hole and the positioning hole in sequence and self-lock to limit the mutual movement between the second sealing plate and the annular boss.

5. The micro-reagent sample loading bottle according to claim 2, characterized in that: A plurality of hinges are arranged between the first sealing plate and the second sealing plate, and the second sealing plate is rotatably connected to the first sealing plate through the hinges.

6. A trace reagent sample loading bottle according to claim 5, characterized in that: A plurality of support blocks are protrudingly formed on the annular boss, the height of the support blocks is greater than the thickness of the hinge, and the bottle body can be placed on the desktop with the mouth of the reagent bottle facing upwards through the support blocks.

7. The micro-reagent sample loading bottle according to claim 1, characterized in that: The sealing column is a rubber cylinder with a diameter larger than the vent hole. The end of the sealing column is tilted inward and recessed to form a chamfered corner for easy matching between the sealing column and the vent hole.

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