Rapid constant-temperature light shielding device for sample dyeing of flow cytometer

Abstract

A rapid constant-temperature light-proof device for sample dyeing of a flow cytometer is characterized in that a refrigerating device is arranged in a main body frame, a control device and a display device are arranged on a platform, a cold air purification device is arranged on the platform, and the cold air purification device is connected with a constant-temperature light-proof box body through a detachable external pipeline and a connector; a box body door is arranged in front of the constant-temperature light-proof box body; a sound-light alarm device is arranged at the upper part of the constant-temperature light-proof box body The constant-temperature light-proof box body is installed on the platform through a rotating stand column, a storage cabinet is arranged in the main body frame, and trundles are arranged at the bottom of the main body frame. The utility model has the advantages that the temperature can be automatically controlled, the preset temperature can be quickly reached, a stable dark light environment can be formed, and the device is suitable for various operation occasions. Convenience is brought to an operator, and the experiment efficiency is improved. Automatic counting, visual display and convenient statistics are achieved, and the device is suitable for simultaneous use of multiple devices in a large-scale laboratory. The automation degree of each link meets the accuracy of experimental conditions, and the reliability of experimental data is improved.

Description

Technical Field

[0001] The utility model relates to the field of medical experimental equipment, in particular to a rapid constant temperature light-shielding device for sample staining of a flow cytometer. Background Art

[0002] The flow cytometry staining procedure usually includes the following key steps: Sample preparation: Collect whole blood or tissue samples and make a single cell suspension. Red blood cell lysis: If red blood cells need to be lysed, use a specific red blood cell lysis solution to treat the cell suspension to remove the effect of red blood cells on staining and analysis. Blocking Fc receptors: Blocking Fc receptors can reduce nonspecific staining. For mice, purified CD16 / CD32 monoclonal antibodies can be used to block nonspecific staining. For humans and rats, purified Ig or serum of the same source and subtype as the fluorescent antibody can be used for blocking. Cell staining: Add fluorescently labeled antibodies according to the recommended dosage in the instructions, mix well and place at 4°C. There are precise requirements for light intensity during incubation.

[0003] Wash and resuspend cells: Add cell stain to resuspend cells, centrifuge the cell suspension at 300g for 5 minutes, and discard the supernatant. Then add an appropriate amount of flow staining buffer to resuspend the cells and prepare for detection and analysis. Detection and analysis: Add the cell suspension to the flow cytometer for detection and analysis. If it is not possible to detect immediately, resuspend the cells in a specific storage solution and store at 4°C for analysis within 24 hours.

[0004] The above steps are the basic process of flow cytometry staining. The specific steps may vary depending on the purpose of the experiment and the type of antibody used. When conducting the experiment, you should strictly follow the detection method to ensure the accuracy and reliability of the experimental results.

[0005] Existing devices usually have estimation problems in temperature control and light intensity control, especially the temperature constancy and the time to quickly reach the predetermined temperature, which usually rely on the operator's experience to roughly judge, and the changes in values ​​during operation are also unknown. Utility Model Content

[0006] The utility model aims to overcome the above problems and provides a rapid constant temperature light-shielding device for sample staining of a flow cytometer.

[0007] The utility model provides a rapid constant temperature and light-proof device for sample staining of a flow cytometer, which is characterized in that: the rapid constant temperature and light-proof device for sample staining of a flow cytometer comprises a main frame 1, a refrigeration device 2, a platform 3, a control device 4, a display device 5, a cold air purification device 6, a detachable external pipeline and interface 7, a constant temperature and light-proof box body 8, a box body door 9, an insulation layer 10, an audible and visual alarm device 11, an exhaust port 12 with a one-way valve, a rotating column 13, a storage cabinet 14, and casters 15;

[0008] Wherein: a refrigeration device 2 is arranged inside the main frame 1, a platform 3 is arranged on the main frame 1, a control device 4 and a display device 5 are arranged on the platform 3, a cold air purification device 6 is arranged on the platform 3, the cold air purification device 6 is connected to a constant temperature and light-proof box 8 through a detachable external pipeline and an interface 7, a box door 9 is arranged in front of the constant temperature and light-proof box 8, an insulation layer 10 is arranged outside the constant temperature and light-proof box 8, an audible and visual alarm device 11 is arranged on the upper part of the constant temperature and light-proof box 8, and an exhaust port 12 with a one-way valve is arranged on the side of the constant temperature and light-proof box 8; the constant temperature and light-proof box 8 is installed on the platform 3 through a rotating column 13, a storage cabinet 14 is arranged inside the main frame 1, and casters 15 are arranged at the bottom of the main frame 1.

[0009] The specific structure of the constant temperature and light-proof box 8 includes: an internal longitudinal pipe 801, an anti-direct blowing wind shield 802, a cold air nozzle 803, an internal transverse pipe 804, a temperature sensor 805 in the box, an outlet pipe 806, a lead screw nut 807, a motor-driven lead screw device 808, a test tube holder 809, a longitudinal slider 810, a test tube holder 811, a photoelectric sensor 812, a lifting horizontal plate 813, a lifting support 814, a heating device 815, a transverse guide rail 816, and a depth translation frame 817;

[0010] The lower end of the internal longitudinal pipe 801 is connected to the detachable external pipe and the interface 7, the internal transverse pipe 804 is connected to the upper end of the internal longitudinal pipe 801, and evenly distributed cold air nozzles 803 are arranged below the internal transverse pipe 804, and a direct blowing prevention wind shield 802 is arranged below the cold air nozzle 803;

[0011] A temperature sensor 805 is provided in the constant temperature light-proof box 8, and the temperature sensor 805 is connected to the control device 4. The temperature sensor 805 is used to monitor the temperature in the constant temperature light-proof box 8 in real time;

[0012] A depth translation frame 817 is provided at the lower part of the constant temperature and light-proof box 8, the lower left part of the depth translation frame 817 is connected to the lead screw nut 807, the lead screw nut 807 is connected to the motor-driven lead screw device 808 arranged at the bottom of the constant temperature and light-proof box 8, the lower right part of the depth translation frame 817 is overlapped on the transverse guide rail 816 arranged at the bottom of the constant temperature and light-proof box 8 through a slider, and the upper part of the depth translation frame 817 is provided with a through hole for placing an experimental test tube through; the test tube holder 809 is fixedly connected to the depth translation frame 817;

[0013] The depth translation frame 817 can enter and exit the constant temperature and light-proof box 8 under the drive of the motor-driven screw device 808, and translate stably, making it convenient for the operator to take and place the experimental test tube.

[0014] The lifting horizontal plate 813 is connected to the two inner grooves of the test tube holder 809 through the longitudinal sliders 810 on both sides, and the lower part of the lifting horizontal plate 813 is installed on the bottom of the constant temperature and light-proof box 8 through the lifting support 814; the lifting horizontal plate 813 is driven by the lifting support 814 to achieve longitudinal fine-tuning to improve the placement stability of experimental test tubes at different heights.

[0015] A plurality of test tube holders 811 are arranged on the lifting horizontal plate 813, and a photoelectric sensor 812 is arranged in each test tube holder 811, and the photoelectric sensor 812 is connected to the control device 4; the photoelectric sensor 812 is used to detect whether there are test tubes placed on the current test tube holder 811, and the number of test tubes in the current constant temperature and light-proof box 8 is intuitively displayed through the display device 5, which is convenient for the operator to organize and record.

[0016] An outlet pipeline 806 is provided on the lower side of the constant temperature and light-proof box 8 , and a heating device 815 is provided at the bottom of the constant temperature and light-proof box 8 , and the heating device 815 is connected to the control device 4 .

[0017] The outlet pipe 806 can release cold air, and the heating device 815 assists in heating when the temperature in the constant temperature and light-proof box 8 is low, and the time for heating to reach the preset temperature is used to achieve a rapid constant temperature effect.

[0018] The box door 9 is a closed translucent material part with an adjustable shutter-type shading device on the outside. The translucent material part can appropriately adjust the intensity of the transmitted light, and the shutter-type shading device can adjust the amount of light entering through the shutters.

[0019] The constant temperature and light-proof box 8 is installed on the front side of the box door 9, and is provided with a light intensity detection sensor. The light intensity detection sensor is connected to the control device 4 and can automatically adjust the rotation angle of the rotating column 13 according to the intensity of the received light, so as to change the incident angle of the constant temperature and light-proof box 8 to receive natural light or light, and assist the shutter-type shading device to further adjust the light intensity inside the constant temperature and light-proof box 8 to meet the light-shielding degree required for different experiments.

[0020] The refrigeration device 2 is a compressor refrigeration device with high efficiency.

[0021] The control device 4 is a programmable logic controller with stable performance and is not easily disturbed by environmental electric and magnetic fields.

[0022] The display device 5 is a touch screen, which is convenient for the operator to adjust the set thresholds of each link and adjust the in and out of the depth translation frame 817, making it convenient for the operator to take and put.

[0023] The heating device 815 is a backup temperature regulating device, specifically a platinum resistance heating device, which has low cost and is easy to control.

[0024] The lifting support 814 is a manual spiral lifting device.

[0025] The light intensity detection sensor is a photoresistor.

[0026] During the operation, after the door is opened and the experimental test tube is placed, the temperature value is visually displayed in real time through the display device 5. When the temperature changes beyond the threshold, the refrigeration device 2 or the heating device 815 is started to stabilize the temperature in the constant temperature and light-proof box 8. If the experimental state is abnormal and exceeds the automatic adjustment capacity of the equipment, the sound and light alarm device 11 is started to remind the operator to make manual adjustments to avoid experimental failure.

[0027] Advantages of the utility model:

[0028] The rapid constant temperature light-proof device for sample staining of the flow cytometer described in the utility model has a structure suitable for a variety of operating occasions, automatically controls the temperature and quickly reaches the preset temperature, and can form a stable dark light environment for different experimental processes. The depth translation frame enters and exits the box body through a screw mechanism, which facilitates the operator's movements and improves the efficiency of the experiment. The automatic counting of experimental test tubes and the intuitive display facilitate statistics, which is suitable for multiple devices in large laboratories to conduct experiments at the same time. The degree of automation of each link meets the accuracy of the experimental conditions and improves the reliability of the experimental data. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] The present invention is further described in detail below with reference to the accompanying drawings and implementation modes:

[0030] Figure 1 This is a schematic diagram of the external structure of a rapid constant temperature light-shielding device for sample staining in a flow cytometer;

[0031] Figure 2 This is a schematic diagram of the internal structure of a stable constant temperature and light-proof box;

[0032] Figure 2 The direction of the middle arrow indicates the direction of the cold air. DETAILED DESCRIPTION

[0033] Example 1

[0034] A rapid constant temperature and light-proof device for sample staining of a flow cytometer, characterized in that: the rapid constant temperature and light-proof device for sample staining of a flow cytometer comprises a main frame 1, a refrigeration device 2, a platform 3, a control device 4, a display device 5, a cold air purification device 6, a detachable external pipeline and interface 7, a constant temperature and light-proof box 8, a box door 9, an insulation layer 10, an audible and visual alarm device 11, an exhaust port 12 with a one-way valve, a rotating column 13, a storage cabinet 14, and casters 15;

[0035] Wherein: a refrigeration device 2 is arranged inside the main frame 1, a platform 3 is arranged on the main frame 1, a control device 4 and a display device 5 are arranged on the platform 3, a cold air purification device 6 is arranged on the platform 3, the cold air purification device 6 is connected to a constant temperature and light-proof box 8 through a detachable external pipeline and an interface 7, a box door 9 is arranged in front of the constant temperature and light-proof box 8, an insulation layer 10 is arranged outside the constant temperature and light-proof box 8, an audible and visual alarm device 11 is arranged on the upper part of the constant temperature and light-proof box 8, and an exhaust port 12 with a one-way valve is arranged on the side of the constant temperature and light-proof box 8; the constant temperature and light-proof box 8 is installed on the platform 3 through a rotating column 13, a storage cabinet 14 is arranged inside the main frame 1, and casters 15 are arranged at the bottom of the main frame 1.

[0036] The specific structure of the constant temperature and light-proof box 8 includes: an internal longitudinal pipe 801, an anti-direct blowing wind shield 802, a cold air nozzle 803, an internal transverse pipe 804, a temperature sensor 805 in the box, an outlet pipe 806, a lead screw nut 807, a motor-driven lead screw device 808, a test tube holder 809, a longitudinal slider 810, a test tube holder 811, a photoelectric sensor 812, a lifting horizontal plate 813, a lifting support 814, a heating device 815, a transverse guide rail 816, and a depth translation frame 817;

[0037] The lower end of the internal longitudinal pipe 801 is connected to the detachable external pipe and the interface 7, the internal transverse pipe 804 is connected to the upper end of the internal longitudinal pipe 801, and evenly distributed cold air nozzles 803 are arranged below the internal transverse pipe 804, and a direct blowing prevention wind shield 802 is arranged below the cold air nozzle 803;

[0038] A temperature sensor 805 is provided in the constant temperature light-proof box 8, and the temperature sensor 805 is connected to the control device 4. The temperature sensor 805 is used to monitor the temperature in the constant temperature light-proof box 8 in real time;

[0039] A depth translation frame 817 is provided at the lower part of the constant temperature and light-proof box 8, the lower left part of the depth translation frame 817 is connected to the lead screw nut 807, the lead screw nut 807 is connected to the motor-driven lead screw device 808 arranged at the bottom of the constant temperature and light-proof box 8, the lower right part of the depth translation frame 817 is overlapped on the transverse guide rail 816 arranged at the bottom of the constant temperature and light-proof box 8 through a slider, and the upper part of the depth translation frame 817 is provided with a through hole for placing an experimental test tube through; the test tube holder 809 is fixedly connected to the depth translation frame 817;

[0040] The depth translation frame 817 can enter and exit the constant temperature and light-proof box 8 under the drive of the motor-driven screw device 808, and translate stably, making it convenient for the operator to take and place the experimental test tube.

[0041] The lifting horizontal plate 813 is connected to the two inner grooves of the test tube holder 809 through the longitudinal sliders 810 on both sides, and the lower part of the lifting horizontal plate 813 is installed on the bottom of the constant temperature and light-proof box 8 through the lifting support 814; the lifting horizontal plate 813 is driven by the lifting support 814 to achieve longitudinal fine-tuning to improve the placement stability of experimental test tubes at different heights.

[0042] A plurality of test tube holders 811 are arranged on the lifting horizontal plate 813, and a photoelectric sensor 812 is arranged in each test tube holder 811, and the photoelectric sensor 812 is connected to the control device 4; the photoelectric sensor 812 is used to detect whether there are test tubes placed on the current test tube holder 811, and the number of test tubes in the current constant temperature and light-proof box 8 is intuitively displayed through the display device 5, which is convenient for the operator to organize and record.

[0043] An outlet pipeline 806 is provided on the lower side of the constant temperature and light-proof box 8 , and a heating device 815 is provided at the bottom of the constant temperature and light-proof box 8 , and the heating device 815 is connected to the control device 4 .

[0044] The outlet pipe 806 can release cold air, and the heating device 815 assists in heating when the temperature in the constant temperature and light-proof box 8 is low, and the time for heating to reach the preset temperature is used to achieve a rapid constant temperature effect.

[0045] The box door 9 is a closed translucent material part with an adjustable shutter-type shading device on the outside. The translucent material part can appropriately adjust the intensity of the transmitted light, and the shutter-type shading device can adjust the amount of light entering through the shutters.

[0046] The constant temperature and light-proof box 8 is installed on the front side of the box door 9, and is provided with a light intensity detection sensor. The light intensity detection sensor is connected to the control device 4 and can automatically adjust the rotation angle of the rotating column 13 according to the intensity of the received light, so as to change the incident angle of the constant temperature and light-proof box 8 to receive natural light or light, and assist the shutter-type shading device to further adjust the light intensity inside the constant temperature and light-proof box 8 to meet the light-shielding degree required for different experiments.

[0047] The refrigeration device 2 is a compressor refrigeration device with high efficiency.

[0048] The control device 4 is a programmable logic controller with stable performance and is not easily disturbed by environmental electric and magnetic fields.

[0049] The display device 5 is a touch screen, which is convenient for the operator to adjust the set thresholds of each link and adjust the in and out of the depth translation frame 817, making it convenient for the operator to take and put.

[0050] The heating device 815 is a backup temperature regulating device, specifically a platinum resistance heating device, which has low cost and is easy to control.

[0051] The lifting support 814 is a manual spiral lifting device.

[0052] The light intensity detection sensor is a photoresistor.

[0053] During the operation, after the door is opened and the experimental test tube is placed, the temperature value is visually displayed in real time through the display device 5. When the temperature changes beyond the threshold, the refrigeration device 2 or the heating device 815 is started to stabilize the temperature in the constant temperature and light-proof box 8. If the experimental state is abnormal and exceeds the automatic adjustment capacity of the equipment, the sound and light alarm device 11 is started to remind the operator to make manual adjustments to avoid experimental failure.

[0054] Example 2

[0055] A rapid constant temperature and light-proof device for sample staining of a flow cytometer, characterized in that: the rapid constant temperature and light-proof device for sample staining of a flow cytometer comprises a main frame 1, a refrigeration device 2, a platform 3, a control device 4, a display device 5, a cold air purification device 6, a detachable external pipeline and interface 7, a constant temperature and light-proof box 8, a box door 9, an insulation layer 10, an audible and visual alarm device 11, an exhaust port 12 with a one-way valve, a rotating column 13, a storage cabinet 14, and casters 15;

[0056] Wherein: a refrigeration device 2 is arranged inside the main frame 1, a platform 3 is arranged on the main frame 1, a control device 4 and a display device 5 are arranged on the platform 3, a cold air purification device 6 is arranged on the platform 3, the cold air purification device 6 is connected to a constant temperature and light-proof box 8 through a detachable external pipeline and an interface 7, a box door 9 is arranged in front of the constant temperature and light-proof box 8, an insulation layer 10 is arranged outside the constant temperature and light-proof box 8, an audible and visual alarm device 11 is arranged on the upper part of the constant temperature and light-proof box 8, and an exhaust port 12 with a one-way valve is arranged on the side of the constant temperature and light-proof box 8; the constant temperature and light-proof box 8 is installed on the platform 3 through a rotating column 13, a storage cabinet 14 is arranged inside the main frame 1, and casters 15 are arranged at the bottom of the main frame 1.

[0057] The specific structure of the constant temperature and light-proof box 8 includes: an internal longitudinal pipe 801, an anti-direct blowing wind shield 802, a cold air nozzle 803, an internal transverse pipe 804, a temperature sensor 805 in the box, an outlet pipe 806, a lead screw nut 807, a motor-driven lead screw device 808, a test tube holder 809, a longitudinal slider 810, a test tube holder 811, a photoelectric sensor 812, a lifting horizontal plate 813, a lifting support 814, a heating device 815, a transverse guide rail 816, and a depth translation frame 817;

[0058] The lower end of the internal longitudinal pipe 801 is connected to the detachable external pipe and the interface 7, the internal transverse pipe 804 is connected to the upper end of the internal longitudinal pipe 801, and evenly distributed cold air nozzles 803 are arranged below the internal transverse pipe 804, and a direct blowing prevention wind shield 802 is arranged below the cold air nozzle 803;

[0059] A temperature sensor 805 is provided in the constant temperature light-proof box 8, and the temperature sensor 805 is connected to the control device 4. The temperature sensor 805 is used to monitor the temperature in the constant temperature light-proof box 8 in real time;

[0060] A depth translation frame 817 is provided at the lower part of the constant temperature and light-proof box 8, the lower left part of the depth translation frame 817 is connected to the lead screw nut 807, the lead screw nut 807 is connected to the motor-driven lead screw device 808 arranged at the bottom of the constant temperature and light-proof box 8, the lower right part of the depth translation frame 817 is overlapped on the transverse guide rail 816 arranged at the bottom of the constant temperature and light-proof box 8 through a slider, and the upper part of the depth translation frame 817 is provided with a through hole for placing an experimental test tube through; the test tube holder 809 is fixedly connected to the depth translation frame 817;

[0061] The depth translation frame 817 can enter and exit the constant temperature and light-proof box 8 under the drive of the motor-driven screw device 808, and translate stably, making it convenient for the operator to take and place the experimental test tube.

[0062] The lifting horizontal plate 813 is connected to the two inner grooves of the test tube holder 809 through the longitudinal sliders 810 on both sides, and the lower part of the lifting horizontal plate 813 is installed on the bottom of the constant temperature and light-proof box 8 through the lifting support 814; the lifting horizontal plate 813 is driven by the lifting support 814 to achieve longitudinal fine-tuning to improve the placement stability of experimental test tubes at different heights.

[0063] A plurality of test tube holders 811 are arranged on the lifting horizontal plate 813, and a photoelectric sensor 812 is arranged in each test tube holder 811, and the photoelectric sensor 812 is connected to the control device 4; the photoelectric sensor 812 is used to detect whether there are test tubes placed on the current test tube holder 811, and the number of test tubes in the current constant temperature and light-proof box 8 is intuitively displayed through the display device 5, which is convenient for the operator to organize and record.

[0064] An outlet pipeline 806 is provided on the lower side of the constant temperature and light-proof box 8 , and a heating device 815 is provided at the bottom of the constant temperature and light-proof box 8 , and the heating device 815 is connected to the control device 4 .

[0065] The outlet pipe 806 can release cold air, and the heating device 815 assists in heating when the temperature in the constant temperature and light-proof box 8 is low, and the time for heating to reach the preset temperature is used to achieve a rapid constant temperature effect.

[0066] The box door 9 is a closed translucent material part with an adjustable shutter-type shading device on the outside. The translucent material part can appropriately adjust the intensity of the transmitted light, and the shutter-type shading device can adjust the amount of light entering through the shutters.

[0067] The constant temperature and light-proof box 8 is installed on the front side of the box door 9, and is provided with a light intensity detection sensor. The light intensity detection sensor is connected to the control device 4 and can automatically adjust the rotation angle of the rotating column 13 according to the intensity of the received light, so as to change the incident angle of the constant temperature and light-proof box 8 to receive natural light or light, and assist the shutter-type shading device to further adjust the light intensity inside the constant temperature and light-proof box 8 to meet the light-shielding degree required for different experiments.

[0068] The refrigeration device 2 is a compressor refrigeration device with high efficiency.

[0069] The control device 4 is a programmable logic controller with stable performance and is not easily disturbed by environmental electric and magnetic fields.

[0070] The heating device 815 is a backup temperature regulating device, specifically a platinum resistance heating device, which has low cost and is easy to control.

[0071] During the operation, after the door is opened and the experimental test tube is placed, the temperature value is visually displayed in real time through the display device 5. When the temperature changes beyond the threshold, the refrigeration device 2 or the heating device 815 is started to stabilize the temperature in the constant temperature and light-proof box 8. If the experimental state is abnormal and exceeds the automatic adjustment capacity of the equipment, the sound and light alarm device 11 is started to remind the operator to make manual adjustments to avoid experimental failure.

[0072] Example 3

[0073] A rapid constant temperature and light-proof device for sample staining of a flow cytometer, characterized in that: the rapid constant temperature and light-proof device for sample staining of a flow cytometer comprises a main frame 1, a refrigeration device 2, a platform 3, a control device 4, a display device 5, a cold air purification device 6, a detachable external pipeline and interface 7, a constant temperature and light-proof box 8, a box door 9, an insulation layer 10, an audible and visual alarm device 11, an exhaust port 12 with a one-way valve, a rotating column 13, a storage cabinet 14, and casters 15;

[0074] Wherein: a refrigeration device 2 is arranged inside the main frame 1, a platform 3 is arranged on the main frame 1, a control device 4 and a display device 5 are arranged on the platform 3, a cold air purification device 6 is arranged on the platform 3, the cold air purification device 6 is connected to a constant temperature and light-proof box 8 through a detachable external pipeline and an interface 7, a box door 9 is arranged in front of the constant temperature and light-proof box 8, an insulation layer 10 is arranged outside the constant temperature and light-proof box 8, an audible and visual alarm device 11 is arranged on the upper part of the constant temperature and light-proof box 8, and an exhaust port 12 with a one-way valve is arranged on the side of the constant temperature and light-proof box 8; the constant temperature and light-proof box 8 is installed on the platform 3 through a rotating column 13, a storage cabinet 14 is arranged inside the main frame 1, and casters 15 are arranged at the bottom of the main frame 1.

[0075] The specific structure of the constant temperature and light-proof box 8 includes: an internal longitudinal pipe 801, an anti-direct blowing wind shield 802, a cold air nozzle 803, an internal transverse pipe 804, a temperature sensor 805 in the box, an outlet pipe 806, a lead screw nut 807, a motor-driven lead screw device 808, a test tube holder 809, a longitudinal slider 810, a test tube holder 811, a photoelectric sensor 812, a lifting horizontal plate 813, a lifting support 814, a heating device 815, a transverse guide rail 816, and a depth translation frame 817;

[0076] The lower end of the internal longitudinal pipe 801 is connected to the detachable external pipe and the interface 7, the internal transverse pipe 804 is connected to the upper end of the internal longitudinal pipe 801, and evenly distributed cold air nozzles 803 are arranged below the internal transverse pipe 804, and a direct blowing prevention wind shield 802 is arranged below the cold air nozzle 803;

[0077] A temperature sensor 805 is provided in the constant temperature light-proof box 8, and the temperature sensor 805 is connected to the control device 4. The temperature sensor 805 is used to monitor the temperature in the constant temperature light-proof box 8 in real time;

[0078] A depth translation frame 817 is provided at the lower part of the constant temperature and light-proof box 8, the lower left part of the depth translation frame 817 is connected to the lead screw nut 807, the lead screw nut 807 is connected to the motor-driven lead screw device 808 arranged at the bottom of the constant temperature and light-proof box 8, the lower right part of the depth translation frame 817 is overlapped on the transverse guide rail 816 arranged at the bottom of the constant temperature and light-proof box 8 through a slider, and the upper part of the depth translation frame 817 is provided with a through hole for placing an experimental test tube through; the test tube holder 809 is fixedly connected to the depth translation frame 817;

[0079] The depth translation frame 817 can enter and exit the constant temperature and light-proof box 8 under the drive of the motor-driven screw device 808, and translate stably, making it convenient for the operator to take and place the experimental test tube.

[0080] The lifting horizontal plate 813 is connected to the two inner grooves of the test tube holder 809 through the longitudinal sliders 810 on both sides, and the lower part of the lifting horizontal plate 813 is installed on the bottom of the constant temperature and light-proof box 8 through the lifting support 814; the lifting horizontal plate 813 is driven by the lifting support 814 to achieve longitudinal fine-tuning to improve the placement stability of experimental test tubes at different heights.

[0081] A plurality of test tube holders 811 are arranged on the lifting horizontal plate 813, and a photoelectric sensor 812 is arranged in each test tube holder 811, and the photoelectric sensor 812 is connected to the control device 4; the photoelectric sensor 812 is used to detect whether there are test tubes placed on the current test tube holder 811, and the number of test tubes in the current constant temperature and light-proof box 8 is intuitively displayed through the display device 5, which is convenient for the operator to organize and record.

[0082] An outlet pipeline 806 is provided on the lower side of the constant temperature and light-proof box 8 , and a heating device 815 is provided at the bottom of the constant temperature and light-proof box 8 , and the heating device 815 is connected to the control device 4 .

[0083] The outlet pipe 806 can release cold air, and the heating device 815 assists in heating when the temperature in the constant temperature and light-proof box 8 is low, and the time for heating to reach the preset temperature is used to achieve a rapid constant temperature effect.

[0084] The box door 9 is a closed translucent material part with an adjustable shutter-type shading device on the outside. The translucent material part can appropriately adjust the intensity of the transmitted light, and the shutter-type shading device can adjust the amount of light entering through the shutters.

[0085] The constant temperature and light-proof box 8 is installed on the front side of the box door 9, and is provided with a light intensity detection sensor. The light intensity detection sensor is connected to the control device 4 and can automatically adjust the rotation angle of the rotating column 13 according to the intensity of the received light, so as to change the incident angle of the constant temperature and light-proof box 8 to receive natural light or light, and assist the shutter-type shading device to further adjust the light intensity inside the constant temperature and light-proof box 8 to meet the light-shielding degree required for different experiments.

[0086] The refrigeration device 2 is a compressor refrigeration device with high efficiency.

[0087] During the operation, after the door is opened and the experimental test tube is placed, the temperature value is visually displayed in real time through the display device 5. When the temperature changes beyond the threshold, the refrigeration device 2 or the heating device 815 is started to stabilize the temperature in the constant temperature and light-proof box 8. If the experimental state is abnormal and exceeds the automatic adjustment capacity of the equipment, the sound and light alarm device 11 is started to remind the operator to make manual adjustments to avoid experimental failure.

Claims

1. A rapid constant temperature and light-shielding device for sample staining of a flow cytometer, characterized in that: The rapid constant temperature and light-proof device for sample staining of a flow cytometer comprises a main frame (1), a refrigeration device (2), a platform (3), a control device (4), a display device (5), a cold air purification device (6), a detachable external pipeline and interface (7), a constant temperature and light-proof box (8), a box door (9), an insulation layer (10), an audible and visual alarm device (11), an exhaust port with a one-way valve (12), a rotating column (13), a storage cabinet (14), and casters (15); The invention relates to a main frame (1) having a refrigeration device (2) arranged inside the main frame, a platform (3) arranged on the main frame (1), a control device (4) and a display device (5) arranged on the platform (3), a cold air purification device (6) arranged on the platform (3), the cold air purification device (6) being connected to a constant temperature and light-proof box (8) via a detachable external pipeline and an interface (7), a box door (9) being arranged in front of the constant temperature and light-proof box (8), a heat preservation layer (10) being arranged outside the constant temperature and light-proof box (8), an audible and visual alarm device (11) being arranged on the top of the constant temperature and light-proof box (8), and an exhaust port (12) with a one-way valve being arranged on the side of the constant temperature and light-proof box (8); the constant temperature and light-proof box (8) being installed on the platform (3) via a rotating column (13), a storage cabinet (14) being arranged inside the main frame (1), and casters (15) being arranged at the bottom of the main frame (1).

2. The rapid constant temperature and light-shielding device for sample staining of flow cytometer according to claim 1, characterized in that: The specific structure of the constant temperature and light-proof box (8) comprises: an internal longitudinal pipe (801), an anti-direct blowing wind shield (802), a cold air nozzle (803), an internal transverse pipe (804), a temperature sensor in the box (805), an outlet pipe (806), a lead screw nut (807), a motor-driven lead screw device (808), a test tube holder (809), a longitudinal slider (810), a test tube holder (811), a photoelectric sensor (812), a lifting horizontal plate (813), a lifting support (814), a heating device (815), a transverse guide rail (816), and a longitudinal translation frame (817); The lower end of the internal longitudinal pipe (801) is connected to a detachable external pipeline and an interface (7), the internal transverse pipe (804) is connected to the upper end of the internal longitudinal pipe (801), and evenly distributed cold air nozzles (803) are arranged below the internal transverse pipe (804), and a direct blowing prevention wind shield (802) is arranged below the cold air nozzles (803); A temperature sensor (805) is provided in the constant temperature light-proof box (8), and the temperature sensor (805) is connected to the control device (4); A depth translation frame (817) is arranged at the lower part of the constant temperature and light-proof box (8); the lower left part of the depth translation frame (817) is connected to a lead screw nut (807); the lead screw nut (807) is connected to a motor-driven lead screw device (808) arranged at the bottom of the constant temperature and light-proof box (8); the lower right part of the depth translation frame (817) is overlapped on a transverse guide rail (816) arranged at the bottom of the constant temperature and light-proof box (8) through a slider; the upper part of the depth translation frame (817) is provided with a through hole for placing an experimental test tube through; the test tube holder (809) is fixedly connected to the depth translation frame (817); The lifting horizontal plate (813) is connected to the two inner sliding grooves of the test tube support (809) through the longitudinal sliders (810) on both sides, and the lower part of the lifting horizontal plate (813) is installed at the bottom of the constant temperature and light-proof box (8) through the lifting support (814); A plurality of test tube holders (811) are arranged on the lifting horizontal plate (813), each test tube holder (811) is provided with a photoelectric sensor (812), and the photoelectric sensor (812) is connected to the control device (4); An outlet pipeline (806) is arranged on the lower side of the constant temperature and light-proof box (8), and a heating device (815) is arranged at the bottom of the constant temperature and light-proof box (8), and the heating device (815) is connected to the control device (4).

3. The rapid constant temperature and light-shielding device for sample staining of flow cytometer according to claim 1, characterized in that: The box door (9) is a closed translucent material part, and is provided with an adjustable shutter-type shading device on the outside.

4. The rapid constant temperature and light-shielding device for sample staining of a flow cytometer according to claim 1, characterized in that: The front side of the box door (9) installed on the constant temperature light-proof box (8) is provided with a light intensity detection sensor, which is connected to the control device (4) and can automatically adjust the rotation angle of the rotating column (13) according to the intensity of the received light, so as to change the reception of natural light or direct light.

5. The rapid constant temperature and light-shielding device for sample staining of flow cytometer according to claim 1, characterized in that: The refrigeration device (2) is a compressor refrigeration device.

6. The rapid constant temperature and light-shielding device for sample staining of a flow cytometer according to claim 1, characterized in that: The control device (4) is a programmable logic controller.

7. The rapid constant temperature and light-shielding device for sample staining of a flow cytometer according to claim 1, characterized in that: The display device (5) is a touch screen.

8. The rapid constant temperature and light-shielding device for sample staining of a flow cytometer according to claim 2, characterized in that: The heating device (815) is a standby temperature regulating device, specifically a platinum resistance heating device.

9. The rapid constant temperature and light-shielding device for sample staining of flow cytometer according to claim 2, characterized in that: The lifting support (814) is a manual spiral lifting device.

10. The rapid constant temperature and light-shielding device for sample staining of flow cytometer according to claim 4, characterized in that: The light intensity detection sensor is a photoresistor.

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