Crop seedling pathogenic bacteria inoculation box

Abstract

The utility model discloses a crop seedling pathogenic bacteria inoculation box. The inoculation box comprises a seedling raising box with a top opening, the spore screen is used for placing and uniformly screening pathogenic bacteria spores; the adjusting frame is arranged on the seedling raising box in a sleeving mode, the spore screen is laid on the top of the adjusting frame, and the adjusting frame can ascend and descend relative to the seedling raising box and is limited to ascend and descend. Spores of powdery mildew pathogenic bacteria are laid on the spore screen, the spore screen is slightly shaken, the spores of the pathogenic bacteria uniformly fall onto leaves of seedlings through meshes in the spore screen, liquid doping is not needed in the whole inoculation process, and the seedling raising box provides a relatively independent and safe inoculation environment for the seedlings; spores directly fall off after being scattered on seedling leaves and are not prone to dripping along with liquid accumulation and flowing, spore germination and colonization are more facilitated, and the inoculation success rate is greatly increased.

Description

Technical Field

[0001] The utility model relates to the technical field of plant disease resistance inoculation, in particular to a pathogen inoculation box. Background Art

[0002] Inoculation is a key step in artificially bringing pathogens into contact with the susceptible parts of host plants, creating conditions for pathogens to invade and induce the host to become ill in order to complete the disease diagnosis process. The specific pathogen inoculation operation for specific crops often needs to be completed manually. Taking melon crops as an example, when the seedlings of melon crops are inoculated with powdery mildew pathogens, the existing technology often uses a method of spraying a spore suspension on the seedlings of melon crops. However, since the spore suspension is easy to accumulate or flow on the leaves, it is difficult to evenly apply the pathogen spores on the crop leaves as required by the method of spraying the spore suspension, which is not conducive to the germination and colonization of the pathogen spores; and once the spore suspension accumulates into larger droplets and drips from the leaves, the success rate of this pathogen inoculation operation will also drop sharply, and technicians need to replant or re-cultivate seedlings in time for re-testing, which seriously wastes human resources and test resources. Utility Model Content

[0003] Based on this, it is necessary to provide an inoculation box that does not require the aid of a liquid medium and utilizes the gravity of the pathogen spores themselves to make them fall naturally and evenly on the leaves of the seedlings to improve the success rate of pathogen inoculation.

[0004] A crop seedling pathogen inoculation box, the inoculation box comprising:

[0005] Seedling box: with top opening;

[0006] Spore screen: used to place and evenly screen pathogen spores;

[0007] And an adjustment frame: the adjustment frame is sleeved on the seedling box, the spore screen is laid flat on the top of the adjustment frame, and the adjustment frame can be lifted and lowered relative to the seedling box and is limited in lifting and lowering.

[0008] Optionally, the regulating frame includes a frame body, the bottom of the frame body is connected to the top of the frame body to form a channel for the pathogen spores to fall naturally, and the frame body is sleeved in the box body; the spore screen is arranged on the top of the frame body, and the spore screen is detachably connected to the frame body.

[0009] Optionally, the seedling box includes a box body and at least one adjustment groove; the box body has an internal space, and the adjustment groove is opened on the side wall of the box body; the adjustment frame also includes at least one locking protrusion, the locking protrusion is arranged in a one-to-one correspondence with the adjustment groove, each of the locking protrusions is arranged on the frame body, and each of the locking protrusions is connected to the frame body; the locking protrusion can be embedded in the corresponding adjustment groove and slide back and forth along the adjustment groove.

[0010] Optionally, a plurality of the locking protrusions are provided, adjacent locking protrusions are evenly spaced, and all the locking protrusions are provided at the same height position of the side wall of the frame.

[0011] Optionally, two locking protrusions are provided, and the two locking protrusions are provided at positions symmetrical with respect to the central axis of the frame.

[0012] Optionally, the adjustment slot is opened in the box body along the vertical direction.

[0013] Optionally, the adjustment frame further includes at least one stop block, the stop block is arranged in a one-to-one correspondence with the locking protrusion, the stop block and the locking protrusion are detachably connected, and the stop block is used to fix the frame body relative to the box body.

[0014] Optionally, the locking protrusion is a bolt, one end of which is fixedly connected to the frame, and the other end of which protrudes from the frame and can extend from the adjustment groove; the stop block is a nut adapted to the bolt, and the stop block is screwed with the locking protrusion to fix the frame relative to the box.

[0015] Optionally, the seedling box further includes at least one adjusting ruler, and the adjusting rulers are arranged in one-to-one correspondence with the adjusting grooves, and each of the adjusting rulers is arranged near the notch of the adjusting groove.

[0016] Optionally, the adjusting ruler has a scale, and an extending direction of the adjusting ruler is parallel to an opening direction of the adjusting slot.

[0017] The beneficial effects of the technical solution of the utility model are as follows: melon crop seedlings are grown in a nursery box. When the seedlings develop to a suitable period, the spores of the powdery mildew pathogen are spread on the spore screen. The spore screen is gently shaken, and the pathogen spores pass through the mesh holes on the spore screen and fall evenly onto the leaves of the seedlings. The entire inoculation process does not require liquid doping. The nursery box also provides the seedlings with a safe inoculation environment that is relatively independent of the external environment. After the spores are sprinkled on the leaves of the seedlings, they are not easy to accumulate, flow or even drip with the liquid, which is more conducive to spore germination and colonization, and the inoculation success rate is greatly improved. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on the structures shown in these drawings without paying creative work.

[0019] Figure 1 The present invention is a schematic diagram of the overall structure of a powdery mildew pathogen inoculation box for melon crop seedlings provided in one embodiment.

[0020] The realization of the purpose, functional features and advantages of the utility model will be further explained in conjunction with embodiments and with reference to the accompanying drawings. DETAILED DESCRIPTION

[0021] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments of the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0022] It should be noted that all directional indications in the embodiments of the present invention (such as up, down, left, right, front, back...) are only used to explain the relative position relationship, movement status, etc. between the components under a certain specific posture (as shown in the accompanying drawings). If the specific posture changes, the directional indication will also change accordingly.

[0023] In addition, the descriptions of "first", "second", etc. in the present utility model are only used for descriptive purposes and cannot be understood as indicating or implying their relative importance or implicitly indicating the number of technical features indicated. Therefore, the features defined as "first" and "second" may explicitly or implicitly include at least one of the features. In addition, "and / or" in the full text includes three solutions. Taking A and / or B as an example, it includes technical solution A, technical solution B, and technical solution that satisfies both A and B; in addition, the technical solutions between the various embodiments can be combined with each other, but it must be based on the ability of ordinary technicians in this field to implement. When the combination of technical solutions is contradictory or cannot be implemented, it should be deemed that such combination of technical solutions does not exist and is not within the scope of protection required by the present utility model.

[0024] refer to Figure 1 .

[0025] In this specific embodiment, an inoculation box for powdery mildew pathogens of melon crop seedlings is provided, which includes a seedling box 1, a spore screen 2 and an adjustment frame 3: wherein the seedling box 1 has a semi-enclosed internal space opening upward, which is used for centralized cultivation of melon crop seedlings undergoing inoculation tests; the spore screen 2 has mesh holes for placing and evenly screening pathogen spores; the adjustment frame 3 is mounted on the seedling box 1, for supporting the spore screen 2 and adjusting the distance between the spore screen 2 and the seedling box 1, the spore screen 2 is arranged on the top of the adjustment frame 3, and the spore screen 2 and the adjustment frame 3 are detachably connected.

[0026] Before inoculation, it is necessary to cultivate melon crop seedlings that meet the requirements of the current test. Since different melon crop seedlings have different cultivation cycles and cultivation conditions, and melon crop seedlings with different growth cycles also have different heights, a seedling box 1 is provided in this specific embodiment. The internal space in the seedling box 1 can be used to cultivate melon crop seedlings, and for the seedlings growing in the seedling box, the seedling box 1 provides an independent growth environment isolated from the external environment, in which the seedlings can avoid interference from weather factors, pest factors, etc. in the external environment and grow safely; after the seedlings grow to a suitable size, the height position of the spore screen 2 is adjusted by using the adjustment frame 2, and the distance between the spore screen 2 and the seedling leaves is adjusted to ensure that the distance between the spore screen 2 and the seedling leaves is suitable for the spores to be evenly and diffusely scattered. Spread the spores of powdery mildew pathogen on the spore screen 2, and gently shake the spore screen 2, so that the spore screen 2 will evenly screen the spores, and the spores will evenly fall on the leaves of the seedlings. Since no liquid medium is used in this process, there is no need to consider the accumulation, flow or even dripping of droplets after the spores fall on the leaves of the seedlings. The spores stably adhere to the surface of the leaves of the seedlings and grow stably in the seedling box 1 with the seedlings. The spores are easier to germinate and colonize, and the success rate of inoculation of powdery mildew pathogens is improved. If the mesh number of the spore screen used in the current inoculation does not meet the requirements of the pathogen spore concentration of the current test, the current spore screen 2 can be removed, and a screen with a suitable mesh number can be replaced and installed on the top of the adjustment frame 3.

[0027] In this specific embodiment, the seedling box 1 includes a box body 11 and two adjustment slots 12; the box body 11 has a semi-enclosed internal space with an opening facing upward, and the adjustment slots 12 are opened on the side wall of the box body 11. Two adjustment slots 12 are provided, and the two adjustment slots 12 are symmetrically arranged on both sides of the box body 11. The adjustment frame 3 includes a frame body 31, and the bottom of the frame body 31 is connected to the top of the frame body 31 to form a channel for the pathogenic bacteria spores to fall naturally; the spore screen 2 is arranged on the top of the frame body 31, and the spore screen 2 is detachably connected to the frame body 31. The adjustment frame 3 also includes a locking protrusion 32; each locking protrusion 32 is opened in a one-to-one correspondence with the adjustment groove 12; when the frame 31 is nested in the box body 11, each locking protrusion 32 is embedded in the prefabricated corresponding adjustment groove 12, and the specific position of the locking protrusion 32 in the adjustment groove 12 is adjusted, and the position of the frame 31 relative to the box body 11 changes, and the top of the frame 31 rises or falls, driving the spore screen 2 to rise or fall. Such a setting will help to adapt to seedlings of different growth heights, and help the spore screen 2 to leave a certain distance directly from the leaves of the melon crop seedlings, so as to ensure that when the spore screen 2 is shaken, the powdery mildew pathogen spores on the spore screen 2 are evenly and diffusely sprinkled on the leaves of the melon seedlings.

[0028] In this specific embodiment, the adjustment frame 3 also includes two stop blocks 33, which are arranged one by one with the positioning protrusions 32, and the stop blocks 33 and the positioning protrusions 32 are detachably connected, and the stop blocks 33 are used to realize the positioning of the frame 31 relative to the box 11, so as to fix the frame at the expected height position. The frame 31 is nested in the frame 11, the positioning protrusions 32 extend from the adjustment groove 12, and the stop blocks 33 are engaged with the positioning protrusions 32. After engagement, the stop blocks 33 are pressed against the box 11, and the box 11 is squeezed by the stop blocks 33 and fits tightly with the frame 31. The frame 31 does not slide under pressure, and the spore screen 2 stays at the current height position.

[0029] In this specific embodiment, the locking protrusion 32 is a bolt, one end of which is fixedly connected to the frame 31, and the other end thereof protrudes from the frame 31 and can extend from the adjustment slot 32; the stop block 33 is a nut that cooperates with the bolt.

[0030] In this specific embodiment, the seedling box 1 also includes at least one adjusting ruler 13, and the adjusting ruler 13 is arranged in a one-to-one correspondence with the adjusting slot 32, and each adjusting ruler 13 is arranged near the adjusting slot 12. The adjusting ruler 13 has a scale, and the extension direction of the adjusting ruler 13 is parallel to the opening direction of the adjusting slot 12. Under different melon crops or different growth cycles of the same melon crop, the seedlings may have different actual heights. The adjusting ruler 13 is set, the scale on the adjusting ruler 13 is observed, and the height of the spore screen 2 is accurately adjusted according to the scale indication of the adjusting ruler 13 to ensure that the hovering height of the spore screen 2 is adapted to the actual growth height of the current seedlings, so as to obtain a better spore sprinkling effect, thereby improving the success rate of pathogen spore inoculation.

[0031] The above are only preferred embodiments of the present invention, and do not limit the patent scope of the present invention. All equivalent structural changes made by using the contents of the present invention specification and drawings under the utility model concept, or directly / indirectly used in other related technical fields are included in the patent protection scope of the present invention.

Claims

1. A crop seedling pathogen inoculation box, characterized in that: The inoculation box comprises: Seedling box: with top opening; Spore screen: used to place and evenly screen pathogen spores; And an adjustment frame: the adjustment frame is sleeved on the seedling box, the spore screen is laid flat on the top of the adjustment frame, and the adjustment frame can be lifted and lowered relative to the seedling box and is limited in lifting and lowering.

2. The crop seedling pathogen inoculation box according to claim 1, characterized in that: The seedling box includes a box body, and the adjustment frame includes a frame body. The bottom of the frame body is connected to the top of the frame body to form a channel for the natural falling of pathogenic bacteria spores. The frame body is partially sleeved in the box body, and the frame body can drive the spore screen to rise and fall relative to the box body to adjust the distance between the spore screen and the seedling box; the spore screen is arranged on the top of the frame body, and the spore screen is detachably connected to the frame body.

3. The crop seedling pathogen inoculation box as claimed in claim 2, characterized in that: The seedling box also includes at least one adjustment groove; the box body has a semi-enclosed internal space opening upward, and the adjustment groove is opened on the side wall of the box body; the adjustment frame also includes a locking protrusion, and the locking protrusions are arranged in a one-to-one correspondence with the adjustment grooves, and each of the locking protrusions is fixedly connected to the frame body; the locking protrusions are embedded in the corresponding adjustment grooves, and the locking protrusions slide back and forth along the adjustment grooves, so that the frame body can drive the spore screen to rise and fall relative to the box body.

4. The crop seedling pathogen inoculation box as claimed in claim 3, characterized in that: A plurality of the locking protrusions are provided, and adjacent locking protrusions are evenly spaced, and all the locking protrusions are provided at the same height position of the side wall of the frame body, and the number of the adjustment slots is the same as the number of the locking protrusions, and the adjustment slots correspond to the locking protrusions one by one.

5. The crop seedling pathogen inoculation box as claimed in claim 4, characterized in that: Two locking protrusions are provided, and the two locking protrusions are provided at positions symmetrical with respect to the central axis of the frame.

6. The crop seedling pathogen inoculation box according to claim 4, characterized in that: The adjusting groove is vertically opened on the box body.

7. The crop seedling pathogen inoculation box according to claim 6, characterized in that: The adjustment frame further comprises a stop block, which is arranged in one-to-one correspondence with the locking protrusions, and the stop block and the locking protrusions are detachably connected, and the stop block is used to fix the frame body relative to the box body.

8. The crop seedling pathogen inoculation box according to claim 7, characterized in that: The locking protrusion is a bolt, one end of which is fixedly connected to the frame, and the other end of which protrudes from the frame and can extend from the adjustment groove; the stop block is a nut adapted to the bolt, and the stop block is screwed with the locking protrusion to fix the frame relative to the box.

9. The crop seedling pathogen inoculation box according to claim 3, characterized in that: The seedling raising box also includes at least one adjusting ruler, which is arranged in a one-to-one correspondence with the adjusting grooves, and each of the adjusting rulers is arranged near the notch of the adjusting groove.

10. The crop seedling pathogen inoculation box according to claim 9, characterized in that: The adjusting ruler has scales, and the extending direction of the adjusting ruler is parallel to the opening direction of the adjusting slot.

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