Cell preparation filling device with filter screen

Abstract

The utility model discloses a cell preparation filling device with a filter screen, and particularly relates to the technical field of stem cell preparation filling. A cell preparation filling device with a filter screen comprises a thick pipe and a thin pipe, an inverted circular truncated cone-shaped pipe is arranged between the thick pipe and the thin pipe, the thick pipe, the inverted circular truncated cone-shaped pipe and the thin pipe are integrally formed and form a filling pipe, a movable filter assembly is arranged in the thick pipe and comprises an annular frame, the annular frame is provided with a filter screen, and the filter screen is arranged in the annular frame. A cross-shaped plate is fixedly installed at the bottom of the inner ring of the annular frame, and a filter screen is fixedly installed at the bottom of the annular frame. According to the cell preparation filling device with the filter screen, due to the design of the filter assembly, the filter assembly is more convenient to mount, dismount or move, and the operation efficiency is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of stem cell preparation filling, in particular to a cell preparation filling device with a filter screen. Background Art

[0002] The traditional stem cell preparation filling process involves multiple steps. First, the cells after centrifugal washing are added to the solvent for resuspending. Then, a 100 um pore size screen is placed at the mouth of the new container, and the cell suspension is filtered into a new transfer container through the screen. Finally, the filtered cell suspension in the transfer container is transferred to a disposable preparation filling funnel for preparation filling. This process requires manual grasping and placement of the screen, and the addition of a transfer container as a transition.

[0003] However, the above operation has the following disadvantages: the additional transfer container increases the risk of microbial contamination because each transfer may introduce a new source of contamination.

[0004] Therefore, it is necessary to provide a cell preparation filling device with a filter to solve the above technical problems. Utility Model Content

[0005] In view of the above situation, in order to overcome the defects of the prior art, the utility model provides a design of a filter assembly of a cell preparation filling device with a filter screen, which makes it more convenient to install, disassemble or move the filter assembly and improves the operating efficiency.

[0006] In order to achieve the above purpose, the technical solution adopted by the utility model is as follows:

[0007] A cell preparation filling device with a filter screen comprises: a thick tube and a thin tube, an inverted truncated cone tube is arranged between the thick tube and the thin tube, the thick tube, the inverted truncated cone tube and the thin tube are integrally formed and constitute a filling tube, a movable filter assembly is arranged inside the thick tube, the filter assembly comprises an annular frame, a cross-shaped plate is fixedly installed at the bottom of the inner circle of the annular frame, and a filter screen is fixedly installed at the bottom of the annular frame.

[0008] Preferably, a plurality of fixing rods are fixedly installed on the upper part of the inner ring of the annular frame, and the plurality of fixing rods are fixedly connected to the same threaded block. A pull rod is provided on the threaded block, and a section of external thread is opened on the pull rod. The pull rod is threadedly connected to the threaded block, and the length of the pull rod is longer than the length of the thick tube.

[0009] Preferably, a plugging assembly is arranged below the filter assembly, and the plugging assembly is used to plug the capillary tube. The plugging assembly comprises a threaded sleeve fixed to the bottom of the cross-shaped plate, a connecting rod is threadedly mounted on the threaded sleeve, a plug is fixedly mounted on the bottom of the connecting rod, the plug matches the capillary tube, and the plug is made of silicone.

[0010] Preferably, a sealing ring and a sealing ring are installed on the outer ring of the annular frame, and two sealings are adopted to improve the sealing between the annular frame and the inside of the thick pipe.

[0011] Preferably, the thick tube is made of a transparent material, and a plurality of scale lines are arranged on its outer wall, through which the volume of the filled liquid can be known.

[0012] Preferably, a support handle is fixedly mounted on the upper opening of the thick tube, and the setting of the support handle makes it easy to place the device on the filling bracket and to take it out.

[0013] Compared with the prior art, the utility model has the following beneficial effects:

[0014] (1) The utility model avoids the use of transfer containers by pre-arranging a filter in the filling tube, reduces the risk of microbial contamination, and improves the quality and safety of stem cells.

[0015] (2) The design of the filter assembly in the present invention makes it more convenient to install, disassemble or move the filter assembly, thereby improving operating efficiency.

[0016] (3) The blocking component in the utility model can block the capillary tube when necessary to prevent leakage of cell fluid and ensure the safety of the filling process.

[0017] (4) The sealing design between the annular frame and the thick tube in the utility model improves the sealing performance and prevents the leakage of cell fluid.

[0018] (5) The thick tube of the utility model is made of transparent material, and a plurality of scale lines are arranged on the outer wall, so as to facilitate observation of the volume of the filled liquid.

[0019] (6) In the utility model, a handle is fixedly installed on the upper end of the thick tube, which is convenient for placing the device on the filling bracket and also convenient for taking it out. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 A schematic diagram of the structure of a cell preparation filling device with a filter provided by the utility model;

[0021] Figure 2 for Figure 1 A schematic cross-sectional view of a cell preparation filling device with a filter is shown;

[0022] Figure 3 for Figure 2 Schematic diagram of some structures;

[0023] Figure 4 It is the assembly drawing of the filter assembly and the connecting rod;

[0024] Figure 5 Schematic diagram of the filter assembly.

[0025] Among them, the names corresponding to the figure numbers are: 1-thick tube, 2-inverted truncated cone tube, 3-thin tube, 4-ring frame, 5-cross plate, 6-filter screen, 7-fixing rod, 8-thread block, 9-pull rod, 10-external thread, 11-threaded sleeve, 12-connecting rod, 13-plug, 14-sealing ring, 15-sealing ring, 16-scale line, 17-handle. DETAILED DESCRIPTION

[0026] The present invention is further described below in conjunction with the accompanying drawings and embodiments. The present invention includes but is not limited to the following embodiments.

[0027] Example 1

[0028] like Figure 1-5 As shown, the cell preparation filling device with filter screen provided by the utility model comprises: a thick tube 1 and a thin tube 3, an inverted truncated cone tube 2 is arranged between the thick tube 1 and the thin tube 3, the thick tube 1, the inverted truncated cone tube 2 and the thin tube 3 are integrally formed and form a filling tube, a movable filter assembly is arranged inside the thick tube 1, the filter assembly comprises an annular frame 4, a cross-shaped plate 5 is fixedly installed at the bottom of the inner circle of the annular frame 4, and a filter screen 6 is fixedly installed at the bottom of the annular frame 4. When in use, the filter assembly is installed inside the thick tube 1, and the cell suspension after centrifugal cleaning and solvent re-suspending is poured into the inside of the thick tube 1, and after filtering by the filter assembly, it is directly filled into the preparation bag from the thin tube 3 at the bottom. This embodiment avoids the use of a transfer container by pre-arranging a filter screen in the filling tube, reduces the risk of microbial contamination, and improves the quality and safety of stem cells.

[0029] Example 2

[0030] like Figure 2As shown, a plurality of fixing rods 7 are fixedly installed on the upper part of the inner ring of the annular frame 4, and the plurality of fixing rods 7 are fixedly connected to the same threaded block 8. A pull rod 9 is arranged on the threaded block 8, and a section of external thread 10 is opened on the pull rod 9. The pull rod 9 is threadedly connected with the threaded block 8. The length of the pull rod 9 is longer than the length of the thick tube 1. When installing, disassembling or moving the filter assembly, it is necessary to operate through the pull rod 9. For example, when installing, the pull rod 9 is first installed on the threaded block 8, and then the filter assembly is inserted into the thick tube 1. By pushing the pull rod 9, the filter assembly is pushed to a suitable position, and then the pull rod 9 is rotated to remove it from the threaded block 8. At this time, the installation of the filter assembly is completed; when the pull rod 9 needs to be removed, the pull rod 9 is first threadedly connected with the threaded block 8, and then the pull rod 9 is pulled to remove the filter assembly from the thick tube 1; when the position of the filter assembly needs to be adjusted, the pull rod 9 is installed on the threaded block 8, and the position of the filter assembly can be achieved by appropriately pushing or pulling the pull rod 9.

[0031] Example 3

[0032] like Figure 4 As shown, a plugging component is arranged below the filter component, and the plugging component is used to plug the capillary 3. During filling, if there is a special situation and the filling needs to be stopped in time, the plugging component can be used to plug the capillary 3; there are also some cell fluids that filter slowly and need to gather a certain amount before filling. In this case, the capillary 3 also needs to be plugged in advance, and the plugging component includes a threaded sleeve 11 fixed to the bottom of the cross rod 5, a connecting rod 12 is threadedly installed on the threaded sleeve 11, a plug 13 is fixedly installed on the bottom of the connecting rod 12, and the plug 13 matches the capillary 3. The plug 13 is made of silicone. When the capillary 3 needs to be plugged, the filter component can be moved by pushing the pull rod 9, and the filter component drives the connecting rod 12 to move through the threaded sleeve 11, and the movement of the connecting rod 12 drives the plug 13 to move, so that the plug 13 enters the capillary 3. When filling is required, the plug 13 can be drawn out from the capillary 3 by simply pulling the pull rod 9 upwards, thereby achieving filling.

[0033] Example 4

[0034] like Figure 3 and 5 As shown, a sealing ring 14 and a sealing ring 15 are installed on the outer ring of the annular frame 4, and two sealings are adopted to improve the sealing between the annular frame 4 and the inside of the thick pipe 1.

[0035] Example 5

[0036] like Figure 1 As shown, the thick tube 1 is made of transparent material, and a plurality of scale lines 16 are arranged on its outer wall. The plurality of scale lines 16 can be used to know the volume of the filled liquid.

[0037] Example 6

[0038] like Figure 1 As shown, a support handle 17 is fixedly installed on the upper opening of the thick tube 1. The setting of the support handle 17 makes it easy to place the device on the filling bracket and to take it out.

[0039] Working principle: When the cell suspension needs to be filled, first install the connecting rod 12 on the threaded sleeve 11, then thread the pull rod 9 on the threaded block 8, and then insert the filter assembly into the thick tube 1 until it is inserted into the appropriate position (the specific position depends on the situation. If the thin tube needs to be blocked in advance, the plug 13 needs to be inserted into the thin tube 3 in advance), and then place the device on the filling bracket. At this time, pour an appropriate amount of cell suspension into the thick tube 1, and the filtered cell suspension is directly filled into the preparation bag through the thin tube 3.

Claims

1. A cell preparation filling device with a filter, characterized in that: include: A thick tube (1) and a thin tube (3), an inverted truncated cone-shaped tube (2) is arranged between the thick tube (1) and the thin tube (3), the thick tube (1), the inverted truncated cone-shaped tube (2) and the thin tube (3) are integrally formed and constitute a filling tube, a movable filter assembly is arranged inside the thick tube (1), the filter assembly comprises an annular frame (4), a cross-shaped plate (5) is fixedly mounted on the bottom of the inner ring of the annular frame (4), and a filter screen (6) is fixedly mounted on the bottom of the annular frame (4).

2. A cell preparation filling device with a filter according to claim 1, characterized in that: A plurality of fixing rods (7) are fixedly mounted on the upper part of the inner ring of the annular frame (4). The plurality of fixing rods (7) are fixedly connected to the same threaded block (8). A pull rod (9) is provided on the threaded block (8). The pull rod (9) is provided with a section of external thread (10). The pull rod (9) is threadedly connected to the threaded block (8). The length of the pull rod (9) is longer than the length of the thick tube (1).

3. A cell preparation filling device with a filter according to claim 1, characterized in that: A plugging assembly is provided below the filter assembly, the plugging assembly being used to plug the capillary tube (3), the plugging assembly comprising a threaded sleeve (11) fixed to the bottom of the cross-shaped plate (5), a connecting rod (12) being threadedly mounted on the threaded sleeve (11), a plug (13) being fixedly mounted at the bottom of the connecting rod (12), the plug (13) matching the capillary tube (3).

4. The cell preparation filling device with a filter according to claim 1, characterized in that: A sealing ring (14) and a sealing ring (15) are mounted on the outer ring of the annular frame (4).

5. The cell preparation filling device with a filter according to claim 1, characterized in that: A plurality of scale lines (16) are provided on the outer wall of the thick tube (1).

6. The cell preparation filling device with a filter according to claim 1, characterized in that: A support handle (17) is fixedly mounted on the upper opening of the thick tube (1).

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