Bearing module of nucleic acid extraction and amplification kit

Abstract

The utility model provides a bearing module of a nucleic acid extraction and amplification kit, which comprises a bearing groove for bearing the nucleic acid extraction and amplification kit, and a buckle component, a heating component and a magnetic component are arranged in the bearing groove; the connecting piece, the shifting block, the guide rail and the tension spring structure are designed on the buckle assembly, so that the nucleic acid extraction and amplification kit can be mounted, fixed and dismounted very conveniently, and the buckle assembly can automatically return to the original position after being dismounted; through the structural design of the magnetic attraction assembly, the magnetic attraction function can be accurately controlled only by shifting the sliding block, and the automation effect is improved; through the design of specific heating assemblies for different parts, the temperature control effect is improved, and a guarantee is provided for extraction, amplification and detection of nucleic acid.

Description

Technical Field

[0001] The utility model belongs to the field of biological detection, and specifically relates to a component of a nucleic acid extraction detection device, in particular to a carrier module of a nucleic acid extraction and amplification reagent kit. Background Art

[0002] Nucleic acid extraction and amplification detection are important experimental methods in molecular biology research. Early experiments were all done manually, which took a long time and the experimental results were easily affected by individual differences in experimental personnel. With the development of industrial automation, in the field of experimental science, traditional manual experiments are increasingly replaced by automated equipment. The repeatability and consistency of automated equipment reduce the errors of traditional manual experiments and improve the reliability and efficiency of experiments. The nucleic acid extraction and detection all-in-one machine is a highly integrated compact instrument that performs fully automatic, fully closed, and integrated operations for nucleic acid extraction and amplification. It can highly automate the automation of nucleic acid extraction and amplification detection, shorten the nucleic acid extraction and amplification process, reduce the impact of human factors, enhance the safety and effectiveness of nucleic acid sample preparation, and realize the miniaturization and portability of the device to meet the needs of grassroots or on-site rapid detection. It is the main research direction for the development of new nucleic acid extraction and detection all-in-one machines.

[0003] The nucleic acid extraction and detection all-in-one machine needs to use a nucleic acid extraction and amplification kit to complete the extraction and detection of nucleic acids. Therefore, it is necessary to set up a corresponding carrying module in the nucleic acid extraction and detection all-in-one machine to fix the nucleic acid extraction and amplification kit. At the same time, the structure of the carrying module must be designed so that it can provide corresponding support for the nucleic acid extraction and amplification functions of the nucleic acid extraction and amplification kit, such as providing heating and magnetic suction functions during the nucleic acid extraction and amplification process.

[0004] The nucleic acid extraction and amplification kit is a disposable consumable used in the nucleic acid extraction and detection all-in-one machine, which requires multiple installation and disassembly. Therefore, how to make the nucleic acid extraction and amplification kit easier to install, firmly locked when fixed, and easily removed when disassembled, and how to repeat the installation and disassembly work many times without affecting the performance of the nucleic acid extraction and amplification kit and the carrier module is an urgent problem to be solved.

[0005] In addition, the existing nucleic acid extraction and amplification kits have poor integration, making it difficult to complete the nucleic acid extraction and detection of complex samples, and have low throughput, usually only able to amplify one nucleic acid at a time. When the nucleic acid extraction and amplification kit needs to complete the nucleic acid extraction and detection of complex samples, such as when multiple items need to be detected at the same time, higher requirements will be placed on the temperature control and magnetic suction function control of its carrying module.

[0006] Therefore, it is urgent to find a carrier module with a simple structure and clever design to cooperate with the nucleic acid extraction and amplification kit to complete the nucleic acid extraction and detection of complex samples, and to achieve the installation, fixation and disassembly of the nucleic acid extraction and amplification kit more efficiently and conveniently. Utility Model Content

[0007] In order to solve the above problems, the utility model provides a carrying module for a nucleic acid extraction and amplification kit, including a carrying slot for carrying the nucleic acid extraction and amplification kit, wherein a snap assembly, a heating assembly and a magnetic suction assembly are arranged in the carrying slot; by designing a connector, a shifting block, a guide rail and a tension spring structure on the snap assembly, the installation, fixation and disassembly of the nucleic acid extraction and amplification kit can be realized very conveniently, and after disassembly, the snap assembly can also automatically return to its position; through the structural design of the magnetic suction assembly, the magnetic suction function can be accurately controlled by only shifting a slider, thereby improving the automation effect; through the design of specific heating assemblies for different parts, the temperature control effect is improved, thereby providing a guarantee for the extraction, amplification and detection of nucleic acids.

[0008] The utility model provides a carrier module for a nucleic acid extraction and amplification kit, comprising a carrier slot for carrying the nucleic acid extraction and amplification kit, wherein a snap assembly, a magnetic attraction assembly and a heating assembly are arranged in the carrier slot; the snap assembly is used to snap the carrier module and the nucleic acid extraction and amplification kit together, thereby fixing the nucleic acid extraction and amplification kit; the heating assembly is used to heat the reagents in the nucleic acid extraction and amplification kit, and the magnetic attraction assembly is used to provide magnetic attraction for the magnetic beads in the nucleic acid extraction and amplification kit.

[0009] Furthermore, the snap assembly includes a snap plate, a connecting piece and a snap block, the snap plate includes a first snap plate and a second snap plate, the connecting piece is used to connect the first snap plate and the second snap plate, the snap block is connected to the second snap plate as a whole, and moving the snap block can move the first snap plate, the connecting piece and the second snap plate simultaneously.

[0010] In some embodiments, moving the buckle block refers to moving the buckle block from the leftmost side to the rightmost side.

[0011] Furthermore, a first snap-in rod is provided on the first snap-in plate, extending outwardly perpendicularly to the first snap-in plate, and is used to be snap-connected with the first snap-in of the nucleic acid extraction and amplification kit; a second snap-in rod is provided on the second snap-in plate, extending outwardly perpendicularly to the second snap-in plate, and is used to be snap-connected with the second snap-in of the nucleic acid extraction and amplification kit; when the nucleic acid extraction and amplification kit is put in, the snap-in block is toggled so that the first snap-in rod and the second snap-in rod can be respectively snapped with the first snap and the second snap-in of the nucleic acid extraction and amplification kit.

[0012] Nucleic acid extraction and amplification kits are usually disposable consumables, and a new nucleic acid extraction and amplification kit needs to be replaced for each test. Each nucleic acid extraction and amplification kit is provided with buckles on both sides of the bottom. When the nucleic acid extraction and amplification kit is loaded into the nucleic acid extraction and detection all-in-one machine, it needs to be installed in the carrying module. The two buckles are respectively combined and fixed with the buckle components of the carrying module.

[0013] The buckle shape of the nucleic acid extraction and amplification kit is a "7" shape, with a "—"-shaped track on the top and a "|"-shaped track on the bottom, and the upper "—" is relatively long. When the nucleic acid extraction and amplification kit is loaded into the carrier module, the buckle rod in the carrier module can just enter along the "|" track and reach the highest end of the "|" track, so that the buckle and the buckle assembly are buckled; at this time, when the buckle dial block is turned, the buckle rod can slide along the "—" to the deepest point, so that the buckle and the buckle assembly are completely combined and fixed; when the buckle dial block is turned in the opposite direction, the buckle rod can slide out along the "—" to the highest end of the "|"-shaped track, and the nucleic acid extraction and amplification kit can be directly taken out.

[0014] The buckle assembly provided by the utility model can make the buckles just buckle with the buckle assembly when the nucleic acid extraction and amplification kit is loaded into the carrier module, and the nucleic acid extraction and amplification kit can be completely locked by toggling the buckle block. When it needs to be taken out, it only needs to toggle the buckle block in the opposite direction to easily take it out, which is very convenient and quick. After repeatedly completing the installation and disassembly work for many times, it can still be easily installed, locked and disassembled, and the performance of the nucleic acid extraction and amplification kit and the carrier module will not be affected.

[0015] In some embodiments, the snap plate is a square plate, the snap rod is a cylindrical structure, and is located in the middle of the snap plate. The cross-sectional diameter of the cylinder matches the size of the "7"-shaped snap track.

[0016] Furthermore, the buckle assembly is also provided with a tension spring and a guide rail, one end of the tension spring is fixed on the carrying module, and the other end is fixed to one side below the connecting piece. When the nucleic acid extraction and amplification kit is taken out, the tension spring can automatically return the buckle assembly to its position; the guide rail is located on the other side below the connecting piece, so that the buckle assembly slides along the guide rail.

[0017] In some embodiments, the tension spring can cause the buckle assembly to automatically return to its original position, including the buckle pull block returning from the rightmost side to the leftmost side.

[0018] In some embodiments, a fixing piece is provided on the bottom surface of the carrier module, and one end of the tension spring is connected and fixed to the fixing piece; a fixing plate is also provided below the connecting piece, and the other end of the tension spring is connected and fixed to the fixing plate.

[0019] The setting of the guide rail enables the snap assembly to slide strictly along the track of the guide rail during the process of combining with or disassembling the nucleic acid extraction and amplification kit, without any swaying or shaking back and forth, thereby making the nucleic acid extraction and amplification kit easier to install, fix and disassemble, and the whole process is smoother and effortless.

[0020] Furthermore, the heating assembly includes a lysis zone heating block, a reagent zone heating block and an amplification reaction zone heating block; the lysis zone heating block includes a lysis zone slot and a first ceramic heating rod, the lysis zone slot is used to accommodate the lysis zone in the nucleic acid extraction and amplification kit, and the first ceramic heating rod is arranged below the lysis zone slot; the reagent zone heating block includes a reagent zone heating slot and a second ceramic heating rod, the reagent zone heating slot is provided with a paraffin zone slot and an eluent zone slot, which are respectively used to accommodate the paraffin reagent bottle and the eluent reagent bottle in the nucleic acid extraction and amplification kit, and the second ceramic heating rod is arranged below the reagent zone heating slot, and is used to heat the paraffin reagent bottle and the eluent reagent bottle at the same time.

[0021] Furthermore, a first temperature fuse and a first temperature sensor are provided between the lysis zone tank and the first ceramic heating rod to improve the temperature control accuracy of the lysis zone; a second temperature fuse and a second temperature sensor are provided between the reagent zone heating tank and the second ceramic heating rod to improve the temperature control accuracy of the paraffin reagent bottle and the eluent reagent bottle.

[0022] Furthermore, the amplification reaction zone heating block includes an eight-tube zone slot and a PCB heating plate. The PCB heating plate is in a long strip shape and is located below the eight-tube zone slot. The eight-tube zone slot is also provided with a fluorescence detection hole.

[0023] The utility model adopts different temperature control methods for different areas. For small-area heating of the cracking area and the reagent bottle area, a ceramic rod is used in combination with a temperature fuse and a temperature sensor to control the temperature. For large-area heating and temperature control such as the eight-tube area, a PCB heating plate is used for temperature control. The heating surface is large and the heating is more uniform, which can meet the heating needs of each reaction tube.

[0024] In some embodiments, the PCB heating plate is in the shape of a long strip and is covered with heating wires, so that it can be completely flush with the bottom of the heating block in the amplification reaction area, so that the heating is more evenly distributed.

[0025] In some methods, a temperature fuse and three temperature sensors are also provided on the PCB heating plate to meet the precise temperature control of the eight-connected tube.

[0026] Furthermore, the magnetic attraction component includes a slider, a guide rod and a magnet; the slider is sleeved on the guide rod and can slide along the guide rod; the magnet is located below the guide rod and connected to the guide rod.

[0027] Furthermore, the slider is provided with a protrusion and a small magnet, the protrusion protruding from the upper surface of the supporting module, and by moving the protrusion, the slider can drive the magnet to switch between a position close to the cracking zone slot and a position far away from the cracking zone slot; the small magnet is located on both sides of the slider and is used to adsorb and fix the slider.

[0028] Since the slider can slide along the guide rod, after moving the bump to make it slide from one side to the other, it may be difficult to fix the slider. Through the small magnets on both sides of the slider, the slider can be adsorbed on the side wall of the nearby carrier module (the side wall is made of metal and can be adsorbed by the small magnets) to prevent arbitrary sliding.

[0029] When the magnet is close to the cracking zone slot, the magnet can fit under the cracking zone heating block.

[0030] In some embodiments, the magnet is trapezoidal in shape, and the lower bottom surface is extended so that when it is close to the cracking zone slot position, it can fit more closely with the bottom of the cracking zone heating block, thereby providing a better magnetic attraction effect as much as possible; and when the magnet is far away from the cracking zone slot position, the magnetic attraction effect cannot be generated due to the long distance.

[0031] Furthermore, it also includes a positioning groove, a radio frequency circuit board and a sample hole. There are two positioning grooves, which are located on one side of the upper surface of the carrier module and are used to locate each hole position in the nucleic acid extraction and amplification kit; the radio frequency circuit board is used to detect the RFID information of the nucleic acid extraction and amplification kit; the sample hole is used to help fix the sample to be tested.

[0032] In some embodiments, the positioning groove is a groove body with an opening upward, the wall of the groove body is cylindrical, and the bottom is hemispherical. When the gun tip of the nucleic acid extraction and detection integrated machine is inserted into the groove body, it is calibrated by contacting with the wall and bottom of the groove body, so that the position of each hole in the nucleic acid extraction and amplification kit can be found during pipetting, which helps to accurately position the gun tip and accurately complete complex pipetting work.

[0033] In some embodiments, the number of the positioning grooves is two. Two points can determine a straight line, and the two positioning grooves can accurately calibrate the coordinate position of the nucleic acid extraction and amplification kit in the carrier module, thereby determining the specific hole positions of various reagent bottles in the nucleic acid extraction and amplification kit, and "two" is also the minimum number required to complete accurate positioning.

[0034] The carrier module of the nucleic acid extraction and amplification kit provided by the utility model has the following beneficial effects:

[0035] 1. By designing the structure of the buckled components in the carrier module, the nucleic acid extraction and amplification kit is easier to install, fix and disassemble, and the whole process is smoother and effortless;

[0036] 2. By designing the structure of the magnetic attraction component in the carrier module, the magnet can be switched between the position close to the cracking zone slot and the position far away from the cracking zone slot at will, making the magnetic attraction simpler, more controllable and more effective;

[0037] 3. By designing the structure of the heating component in the load-bearing module, different temperature control methods are adopted for different areas, which can meet the heating needs of different areas and achieve precise temperature control. BRIEF DESCRIPTION OF THE DRAWINGS

[0038] Figure 1 A schematic diagram of the structure of the nucleic acid extraction and amplification kit and the carrier module provided in Example 1 after being combined;

[0039] Figure 2 A schematic diagram of the structure of the nucleic acid extraction and amplification kit and the carrier module after being disassembled provided in Example 1;

[0040] Figure 3 A schematic diagram of the structure of the carrier module provided in Example 1;

[0041] Figure 4 An exploded view of the carrier module provided in Example 1;

[0042] Figure 5 A schematic diagram of the structure of the buckle assembly provided in Example 1;

[0043] Figure 6 A schematic diagram of the structure of the heating block in the cracking zone provided in Example 1;

[0044] Figure 7 A schematic diagram of the structure of the reagent zone heating block provided in Example 1, wherein the left figure is an overall structural diagram of the reagent zone heating block, and the right figure is a cross-sectional diagram of the reagent zone heating block;

[0045] Figure 8 A schematic diagram of the structure of the amplification reaction zone heating block provided in Example 1, wherein the upper figure is an overall structural diagram of the amplification reaction zone heating block, and the lower figure is a cross-sectional diagram of the amplification reaction zone heating block;

[0046] Fig. 9 A schematic diagram of the structure of the PCB heating plate provided in Example 1;

[0047] Fig.10 This is a schematic diagram of the structure of the magnetic attraction component provided in Example 1. DETAILED DESCRIPTION

[0048] The preferred embodiments of the present invention are further described in detail below in conjunction with the accompanying drawings. It should be pointed out that the following embodiments are intended to facilitate the understanding of the present invention and do not have any limiting effect on it. All features disclosed in the embodiments of the present invention, or all steps in the disclosed methods or processes, except for mutually exclusive features and / or steps, can be combined in any way.

[0049] The carrier module of the nucleic acid extraction and amplification kit provided in this embodiment is as follows Figures 1 to 10 As shown, Figure 1 It is a schematic diagram of the structure after the nucleic acid extraction and amplification kit and the carrier module are combined; Figure 2 This is a schematic diagram of the structure of the nucleic acid extraction and amplification kit and the carrier module after being disassembled; Figure 3 It is a structural schematic diagram of the carrier module; Figure 4 This is the exploded view of the load-bearing module; Figure 5 is a structural schematic diagram of a buckle assembly; Figure 6 It is a schematic diagram of the structure of the heating block in the cracking zone; Figure 7 It is a schematic diagram of the structure of the heating block in the reagent area; Figure 8 A cross-sectional view of a heating block in an amplification reaction area; Fig. 9 It is a structural schematic diagram of the PCB heating plate; Fig.10 It is a structural schematic diagram of the magnetic suction component.

[0050] like Figures 1 to 4 As shown, the carrier module 1 provided in this embodiment is located in the nucleic acid extraction and detection integrated machine, and is used to carry the nucleic acid extraction and amplification kit 3, and provide heating, magnetic suction functions, etc. for the nucleic acid extraction and amplification kit 3 during the nucleic acid extraction and amplification process. The carrier module 1 includes a carrier slot 4 for carrying the nucleic acid extraction and amplification kit 3, and a snap assembly 5, a magnetic suction assembly 6 and a heating assembly 7 are provided in the carrier slot 4; the snap assembly 5 is used to snap the carrier module 1 and the nucleic acid extraction and amplification kit 3 together, thereby fixing the nucleic acid extraction and amplification kit 3; the heating assembly 7 is used to heat the reagents in the nucleic acid extraction and amplification kit 3, and the magnetic suction assembly 6 is used to provide magnetic suction for the magnetic beads in the nucleic acid extraction and amplification kit 3.

[0051] like Figure 5 As shown, the buckle assembly 5 includes a buckle plate 8, a connecting member 9 and a buckle block 10. The buckle plate 8 includes a first buckle plate 11 and a second buckle plate 12. The connecting member 9 is used to connect the first buckle plate 11 and the second buckle plate 12. The buckle block 10 is connected to the second buckle plate 12 as a whole. The first buckle plate 11, the connecting member 9 and the second buckle plate 12 can be moved simultaneously by moving the buckle block 12. The first buckle plate 11 is provided with a first buckle rod 13, which extends outward perpendicularly to the first buckle plate 11 and is used to buckle with the first buckle 301 of the nucleic acid extraction and amplification reagent kit 3 ( Figure 2, the first buckle is blocked, the first buckle and the second buckle have the same structure and are respectively located on both sides of the nucleic acid extraction and amplification kit); a second buckle rod 14 is provided on the second buckle plate 12, extending outward perpendicularly to the second buckle plate 12, and is used to buckle with the second buckle 302 of the nucleic acid extraction and amplification kit 3; when the nucleic acid extraction and amplification kit 3 is placed in the carrying module 1, the buckle block 10 is toggled to make the first buckle rod 13 and the second buckle rod 14 buckle with the first buckle 301 and the second buckle 302 of the nucleic acid extraction and amplification kit 3 respectively.

[0052] The nucleic acid extraction and amplification kit 3 is usually a disposable consumable. A new nucleic acid extraction and amplification kit 3 needs to be replaced for each test. Each nucleic acid extraction and amplification kit 3 is provided with buckles 30 on both sides below. When the nucleic acid extraction and amplification kit 3 is loaded into the nucleic acid extraction and detection integrated machine, it needs to be installed in the carrier module 1. The two buckles 30 are respectively combined with the buckle components 5 of the carrier module 1 and fixed. Figure 3 The buckle 30 of the nucleic acid extraction and amplification kit 3 is in the shape of a "7", with a "-" shaped track 31 on the top and a "|" shaped track 32 on the bottom, wherein the "-" shaped track 31 on the top is relatively long. When the nucleic acid extraction and amplification kit 3 is loaded into the carrier module 1, the first buckle rod 13 and the second buckle rod 14 in the carrier module 1 can just enter along the "|" track and directly reach the highest end of the "|" track, so that the buckle 30 is buckled with the buckle assembly 5; at this time, when the buckle block 12 is turned, the first buckle rod 13 and the second buckle rod 14 can slide along the "-" to the deepest point, so that the buckle 30 and the buckle assembly 5 are completely combined and fixed; when the buckle block 12 is turned in the opposite direction, the first buckle rod 13 and the second buckle rod 14 can slide out along the "-" to the highest end of the "|" shaped track, and the nucleic acid extraction and amplification kit 3 can be directly taken out. The buckle assembly 5 enables the first buckle 301 and the second buckle 302 to be exactly buckled with the buckle assembly 5 when the nucleic acid extraction and amplification kit 3 is loaded into the carrier module 1. Then, the nucleic acid extraction and amplification kit 3 can be completely locked by turning the buckle block 10. When it needs to be taken out, it only needs to be turned in the opposite direction to easily take it out. It is very convenient and fast. After repeating the installation and disassembly work many times, it can still be easily installed, locked and disassembled, and will not affect the performance of the nucleic acid extraction and amplification kit 3 and the carrier module 1.

[0053] Preferably, the first snap plate 11 and the second snap plate 12 are both square plates, the first snap rod 13 and the second snap rod 14 are both cylindrical structures, located in the middle of the snap plate 8, and the cross-sectional diameter of the cylinder matches the size of the "7"-shaped snap track. The snap assembly 5 is also provided with a tension spring 16 and a guide rail 17, one end 161 of the tension spring 16 is fixed to the carrier module 1, and the other end is fixed to one side 901 below the connector 9. When the nucleic acid extraction and amplification kit 3 is taken out, the tension spring 16 can automatically return the snap assembly 5 to its original position; the guide rail 17 is located at the other side 902 below the connector 9, so that the snap assembly 5 slides along the guide rail 17. A fixing member 18 is provided on the bottom surface of the carrier module 1, and one end 161 of the tension spring 16 is connected and fixed to the fixing member 18; a fixing plate is also provided below the connector 9, and the other end of the tension spring 16 is connected and fixed to the fixing plate. The guide rail 17 enables the buckle assembly 5 to slide strictly along the track of the guide rail 17 during the process of combining with or disassembling the nucleic acid extraction and amplification reagent kit 3, without any swaying or shaking, thereby making the nucleic acid extraction and amplification reagent kit 3 easier to install, fix and disassemble, and the whole process is smoother and effortless.

[0054] like Figures 3 to 9 The heating assembly 7 includes a lysis zone heating block 20, a reagent zone heating block 21 and an amplification reaction zone heating block 22; the lysis zone heating block 20 includes a lysis zone slot 23 and a first ceramic heating rod 24, the lysis zone slot 23 is used to accommodate the lysis zone 303 in the nucleic acid extraction and amplification kit 3, and the first ceramic heating rod 24 is arranged below the lysis zone slot 23; the reagent zone heating block 21 includes a reagent zone heating slot 25 and a second ceramic heating rod 26, the reagent zone heating slot 25 is provided with a paraffin zone slot 26 and an eluent zone slot 27, which are respectively used to accommodate the paraffin reagent bottle 304 and the eluent reagent bottle 305 in the nucleic acid extraction and amplification kit 3, and the second ceramic heating rod 26 is arranged below the reagent zone heating slot 25, and is used to heat the paraffin reagent bottle 304 and the eluent reagent bottle 305 at the same time.

[0055] like Figure 6 and 7 A first temperature fuse 28 and a first temperature sensor 29 are provided between the cracking zone tank 23 and the first ceramic heating rod 24 to improve the temperature control accuracy of the cracking zone 303; a second temperature fuse 33 and a second temperature sensor 34 are provided between the reagent zone heating tank 25 and the second ceramic heating rod 26 to improve the temperature control accuracy of the paraffin reagent bottle 304 and the eluent reagent bottle 305. Figure 8The amplification reaction zone heating block 22 includes an eight-tube zone slot 35 and a PCB heating plate 36. The PCB heating plate 36 is in the shape of an elongated strip and is located below the eight-tube zone slot 35. The eight-tube zone slot 35 is also provided with a fluorescence detection hole 37 for fluorescence detection of the target nucleic acid. This embodiment adopts different temperature control methods for different areas. For small-area heating of the lysis zone 303 and the reagent bottle zone 306, a ceramic rod is used in combination with a temperature fuse and a temperature sensor for temperature control; and for large-area heating and temperature control such as the eight-tube zone 307, a PCB heating plate 36 is used for temperature control. The heating surface is large and the heating is more uniform, which can meet the heating requirements of each eight-tube 38. Fig. 9 The PCB heating plate 36 is long and strip-shaped, and is covered with heating wires, which can be completely flush with the bottom of the amplification reaction zone heating block 22, so that the heating is more uniform. The PCB heating plate 36 is also provided with a third temperature fuse 40 and three third temperature sensors 41, so as to meet the precise temperature control of the eight-connection tube 38.

[0056] like Fig.10 As shown, the magnetic attraction assembly 6 includes a slider 42, a guide rod 43 and a magnet 44; the slider 42 is sleeved on the guide rod 43 and can slide along the guide rod 43; the magnet 44 is located below the guide rod 43 and connected to the guide rod 43. The slider 42 is provided with a protrusion 45, which extends from the upper surface 46 of the carrier module 1. By moving the protrusion 45, the slider 42 can drive the magnet 44 to switch between a position close to the cracking zone slot 23 and a position away from the cracking zone slot 23; when the magnet 44 is close to the cracking zone slot 23, the magnet 44 can fit under the cracking zone heating block 20. The magnet 44 is trapezoidal, and the lower bottom surface 47 is extended and extended, so that when it is close to the cracking zone slot 23, it can fit more closely under the cracking zone heating block 20, providing a better magnetic attraction effect as much as possible; and when the magnet 44 is far away from the cracking zone slot 23, the magnetic attraction effect cannot be generated due to the long distance.

[0057] like Fig.10 , a small magnet 52 is provided on each side of the slider 42, and the small magnets 52 are located on both sides of the slider, and are used to be adsorbed on the side wall of the adjacent carrier module 1 to fix the slider 42. Because the slider 42 can slide along the guide rod 43, after the protrusion 45 is moved to stop it, it may be difficult to fix the slider. The small magnets 52 on both sides of the slider 42 can make the slider 42 adsorbed on the side wall of the adjacent carrier module 1 to prevent it from sliding arbitrarily.

[0058] like Figures 1 to 4, the carrier module 1 is also provided with a positioning groove 48, a radio frequency circuit board 49 and a sample hole 50. There are two positioning grooves 48, which are located on one side of the upper surface 46 of the carrier module 1 and are used to locate each hole in the nucleic acid extraction and amplification kit 3; the radio frequency circuit board 49 is used to detect the RFID information of the nucleic acid extraction and amplification kit 3; the sample hole 50 is used to help fix the sample to be tested. The positioning groove 48 is a groove body 51 with an opening upward, the wall of the groove body 51 is cylindrical, and the bottom is hemispherical. When the gun head 201 of the nucleic acid extraction and detection integrated machine is inserted into the positioning groove 48, it is calibrated by contacting with the wall and bottom of the groove body 51, so that the position of each hole in the nucleic acid extraction and amplification kit 3 can be found when performing pipetting work, which helps the gun head 201 to accurately locate and accurately complete complex pipetting work. There are two positioning grooves 48, and two points can determine a straight line. The two positioning grooves 48 can accurately calibrate the coordinate position of the nucleic acid extraction and amplification kit 3 in the carrier module 1, thereby determining the specific hole positions of various reagent bottles in the nucleic acid extraction and amplification kit 3. At the same time, "two" is also the minimum number required to complete precise positioning.

[0059] The application of the utility model is not limited to this. For example, the scope of application of the utility model can be expanded according to its environmental protection. Any person skilled in the art can make various changes and modifications without departing from the spirit and scope of the utility model. Therefore, the scope of protection of the utility model shall be based on the scope defined by the claims.

Claims

1. A carrier module for a nucleic acid extraction and amplification kit, characterized in that: It comprises a carrying slot for carrying a nucleic acid extraction and amplification kit, wherein a snap assembly, a magnetic attraction assembly and a heating assembly are arranged in the carrying slot; the snap assembly is used to snap the carrying module and the nucleic acid extraction and amplification kit together, thereby fixing the nucleic acid extraction and amplification kit; the heating assembly is used to heat the reagents in the nucleic acid extraction and amplification kit, and the magnetic attraction assembly is used to provide magnetic attraction for the magnetic beads in the nucleic acid extraction and amplification kit.

2. The carrier module according to claim 1, characterized in that: The buckle assembly includes a buckle plate, a connecting piece and a buckle shift block. The buckle plate includes a first buckle plate and a second buckle plate. The connecting piece is used to connect the first buckle plate and the second buckle plate. The buckle shift block is connected to the second buckle plate as a whole. Moving the buckle shift block can move the first buckle plate, the connecting piece and the second buckle plate simultaneously.

3. The carrier module according to claim 2, characterized in that: A first snap-in rod is provided on the first snap-in plate, extending outwardly perpendicularly from the first snap-in plate, and is used to be snap-connected with the first snap-in of the nucleic acid extraction and amplification kit; a second snap-in rod is provided on the second snap-in plate, extending outwardly perpendicularly from the second snap-in plate, and is used to be snap-connected with the second snap-in of the nucleic acid extraction and amplification kit; when the nucleic acid extraction and amplification kit is put in, the snap-in block is toggled so that the first snap-in rod and the second snap-in rod can be respectively snapped with the first snap and the second snap-in of the nucleic acid extraction and amplification kit.

4. The carrier module according to claim 3, characterized in that: The buckle assembly is also provided with a tension spring and a guide rail, one end of the tension spring is fixed on the carrying module, and the other end is fixed to one side below the connecting piece. When the nucleic acid extraction and amplification kit is taken out, the tension spring can automatically return the buckle assembly to its position; the guide rail is located on the other side below the connecting piece, so that the buckle assembly slides along the guide rail.

5. The carrier module according to claim 4, characterized in that: The heating assembly includes a lysis zone heating block, a reagent zone heating block and an amplification reaction zone heating block; the lysis zone heating block includes a lysis zone slot and a first ceramic heating rod, the lysis zone slot is used to accommodate the lysis zone in the nucleic acid extraction and amplification kit, and the first ceramic heating rod is arranged below the lysis zone slot; the reagent zone heating block includes a reagent zone heating slot and a second ceramic heating rod, the reagent zone heating slot is provided with a paraffin zone slot and an eluent zone slot, which are respectively used to accommodate the paraffin reagent bottle and the eluent reagent bottle in the nucleic acid extraction and amplification kit, and the second ceramic heating rod is arranged below the reagent zone heating slot, and is used to heat the paraffin reagent bottle and the eluent reagent bottle at the same time.

6. The carrier module according to claim 5, characterized in that: A first temperature fuse and a first temperature sensor are also provided between the cracking zone slot and the first ceramic heating rod to improve the temperature control accuracy of the cracking zone; a second temperature fuse and a second temperature sensor are also provided between the reagent zone heating slot and the second ceramic heating rod to improve the temperature control accuracy of the paraffin reagent bottle and the eluent reagent bottle.

7. The carrier module according to claim 6, characterized in that: The amplification reaction zone heating block includes an eight-tube zone slot and a PCB heating plate. The PCB heating plate is in a long strip shape and is located below the eight-tube zone slot. The eight-tube zone slot is also provided with a fluorescence detection hole.

8. The carrier module according to claim 7, characterized in that: The magnetic attraction component comprises a slider, a guide rod and a magnet; the slider is sleeved on the guide rod and can slide along the guide rod; the magnet is located below the guide rod and connected to the guide rod.

9. The carrier module according to claim 8, characterized in that: The slider is provided with a bump and a small magnet, and the bump extends from the upper surface of the supporting module. By moving the bump, the slider can drive the magnet to switch between a position close to the cracking zone slot and a position far away from the cracking zone slot; the small magnet is located on both sides of the slider and is used to adsorb and fix the slider.

10. The carrier module according to claim 9, characterized in that: It also includes a positioning groove, a radio frequency circuit board and a sample hole. The number of the positioning grooves is two, which are located on one side of the upper surface of the carrier module and are used to locate each hole position in the nucleic acid extraction and amplification kit; the radio frequency circuit board is used to detect the RFID information of the nucleic acid extraction and amplification kit; The sample hole is used to help fix the sample to be tested.

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