Auxiliary device for tissue cell separation

Abstract

The utility model provides a tissue cell separation auxiliary device, and belongs to the field of tissue cell separation equipment. According to the technical scheme, the egg cleaning device comprises a base and a lifting mechanism arranged on the base, a clamping base is arranged on the lifting mechanism, a cleaning part and an egg illuminating lamp are further arranged on the base, the lifting mechanism comprises a guide vertical rod arranged on the base, a lifting piece is arranged on the periphery of the guide vertical rod, and the clamping base is arranged on the side face of the lifting piece; the clamping base comprises a supporting plate arranged on the lifting part, an egg support is arranged on the supporting plate, an annular vertical plate is arranged on the periphery of the egg support and on the top face of the supporting plate, and an annular extrusion air bag is arranged on the inner side of the annular vertical plate. The embryo egg sampling device has the advantages that the embryo egg sampling device is simple in structure and convenient to use, embryo eggs can be flexibly clamped and fixed, the situation that the embryo eggs are damaged in the sampling process is reduced, the efficiency of the sampling process is improved, and meanwhile the working pressure of experimenters is relieved.

Description

Technical Field

[0001] The utility model relates to a field, in particular to a tissue cell separation auxiliary device. Background Art

[0002] When collecting chicken embryonic tissue, the experimenter usually holds the embryo egg in one hand and uses the other hand to hold an instrument to tear open the embryo eggshell membrane for sampling. However, the outer shell of the embryo egg is relatively fragile. In order to prevent the embryo egg from breaking, the experimenter needs to hold the embryo egg gently and steadily, while ensuring that the other hand can skillfully operate the instrument and tear open the embryo eggshell membrane for sampling. The entire collection process requires a high degree of concentration, but there is still a possibility of embryo egg breakage. The entire collection process is time-consuming and labor-intensive. Utility Model Content

[0003] The utility model aims to provide a tissue cell separation auxiliary device which has a simple structure, is easy to use, can flexibly clamp and fix embryo eggs, reduces the situation where embryo eggs are damaged during the sampling process, improves the efficiency of the sampling process, and also reduces the work pressure of experimenters.

[0004] The utility model is achieved through the following measures:

[0005] A tissue cell separation auxiliary device, characterized in that it comprises a base, a lifting mechanism arranged on the base, a clamping seat is arranged on the lifting mechanism, and a cleaning part and an egg-illuminating lamp are also arranged on the base;

[0006] The lifting mechanism comprises a guide vertical rod arranged on the base, a lifting member is arranged on the periphery of the guide vertical rod, and the clamping seat is arranged on the side of the lifting member;

[0007] The clamping seat includes a support plate arranged on the lifting member, an egg tray is arranged on the support plate, an annular vertical plate is arranged on the periphery of the egg tray and the top surface of the support plate, an annular extrusion airbag is arranged on the inner side of the annular vertical plate, an air inlet pipe and an exhaust pipe are arranged on the side of the annular extrusion airbag, a sealing cover is arranged on the end of the exhaust pipe, an air pump is arranged on the base, and the air inlet pipe is connected to the air pump through a hose.

[0008] The specific features of the utility model also include:

[0009] The lifting member comprises a U-shaped frame arranged on the periphery of the guide vertical rod, the end surfaces of the side plates on both sides of the U-shaped frame are provided with wing plates, a rotating shaft is arranged between the wing plates on both sides, and the two ends of the rotating shaft are respectively penetrated through the wing plates on both sides to be provided with limited stop rings, and a U-shaped turning handle is arranged between the two ends of the rotating shaft;

[0010] The outer surface of the rotating shaft is provided with rubber bumps.

[0011] The front end of the U-shaped frame is provided with a slot penetrating up and down, the bottom edge of the support plate is provided with a U-shaped limiting plug-in, and a side plate on one side of the U-shaped limiting plug-in is inserted into the slot.

[0012] Limiting vertical grooves are arranged on both sides of the guide vertical rod, and limiting strips are arranged on the inner wall of the U-shaped frame corresponding to the limiting vertical grooves, and the limiting strips are arranged in the limiting vertical grooves.

[0013] A rubber cushion layer is arranged inside the U-shaped frame.

[0014] A bending reinforcement plate is arranged between the U-shaped limiting plug-in and the bottom of the support plate.

[0015] When chicken embryo tissue is sampled, first take embryonated eggs hatched to appropriate embryonic age (9-12 days), use the egg lighting lamp to inspect in the dark, if there are embryonated eggs with abundant blood vessels and embryonic bodies moving, it means that the embryonic bodies are well developed, and use a colored pen to mark the air chamber and embryonic body position, then use warm water to clean the eggshell of the selected embryonated eggs in the cleaning part, and then wipe dry with 75% alcohol cotton balls;

[0016] Placing the embryo egg on the egg tray under aseptic conditions, then starting the air pump to inflate the annular extrusion airbag, after the annular extrusion airbag slightly squeezes the egg tray, stopping the air pump, then adjusting the height of the lifting member as needed, during the adjustment, holding the U-shaped frame, rotating the rotating shaft so that the rubber convex block does not contact the guide vertical rod, lifting the height of the U-shaped frame, after adjusting to a suitable height, rotating the rotating shaft so that the rubber convex block contacts the guide vertical rod, and limiting the U-shaped frame by squeezing the rubber convex block, after the U-shaped frame is in place, opening the air chamber with scissors or tweezers in an aseptic manner, removing the eggshell along the edge of the air chamber, tearing off the shell membrane with ophthalmic tweezers to expose the chicken embryo, gently picking up the embryo head with bent tweezers, taking out the embryo, placing it in a sterile plate, and dissecting and taking materials;

[0017] After the tissue sampling is completed, the sealing cover is opened, the gas in the air bag flows out, and the embryo egg is taken out.

[0018] The utility model has the following beneficial effects: simple structure, easy use, and the ability to flexibly clamp and fix embryo eggs, thereby reducing the situation where embryo eggs are damaged during the material collection process, improving the efficiency of the material collection process, and also reducing the work pressure of experimenters. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 It is a structural schematic diagram of an embodiment of the utility model.

[0020] Figure 2 The structure of the embodiment of the utility model is shown in FIG.

[0021] Figure 3The structure of the embodiment of the utility model is shown in FIG.

[0022] Figure 4 The figure shows the internal structure of an embodiment of the present utility model.

[0023] Figure 5 for Figure 4 A partial enlarged view of part A in the middle.

[0024] Figure 6 The structure of the rotating shaft in the embodiment of the utility model is schematically shown.

[0025] Figure 7 It is a schematic diagram of the structure of the components of the embodiment of the utility model.

[0026] Among them, the accompanying drawings are marked as follows: 1. base; 2. lifting mechanism; 3. clamping seat; 4. cleaning part; 5. guide vertical rod; 501, limiting vertical groove; 6. U-shaped frame; 601, slot; 602, limiting strip; 7. support plate; 8. annular vertical plate; 9. egg tray; 10. U-shaped limiting plug-in; 11. bending reinforcement plate; 12. rotating shaft; 1201, rubber bump; 14. U-shaped handle; 15. wing plate; 16. annular extrusion airbag. DETAILED DESCRIPTION

[0027] In order to clearly illustrate the technical features of this solution, this solution is described below through a specific implementation method.

[0028] See also Figure 1-7 , a tissue cell separation auxiliary device, comprising a base 1, a lifting mechanism 2 arranged on the base 1, a clamping seat 3 arranged on the lifting mechanism 2, and a cleaning part 4 and an egg-illuminating lamp also arranged on the base 1;

[0029] The lifting mechanism 2 includes a guide vertical rod 5 arranged on the base 1, a lifting member is arranged on the periphery of the guide vertical rod 5, and a clamping seat 3 is arranged on the side of the lifting member;

[0030] The clamping seat 3 includes a support plate 7 arranged on a lifting member, an egg tray 9 is arranged on the support plate 7, an annular vertical plate 8 is arranged on the outer periphery of the egg tray 9 and the top surface of the support plate 7, an annular extrusion airbag 16 is arranged on the inner side of the annular vertical plate 8, an air inlet pipe and an exhaust pipe are arranged on the side of the annular extrusion airbag 16, a sealing cover is arranged on the end of the exhaust pipe, an air pump is arranged on the base 1, and the air inlet pipe is connected to the air pump through a hose.

[0031] The lifting member includes a U-shaped frame 6 arranged on the periphery of the guide vertical rod 5, and the end surfaces of the side plates on both sides of the U-shaped frame 6 are provided with wing plates 15, a rotating shaft 12 is arranged between the two side wing plates 15, and limited stop rings are arranged at both ends of the rotating shaft 12, and a U-shaped turning handle 14 is arranged between the two ends of the rotating shaft 12;

[0032] The outer surface of the rotating shaft 12 is provided with a rubber bump 1201 .

[0033] A slot 601 penetrating from top to bottom is disposed at the front end of the U-shaped frame 6 , a U-shaped limiting plug-in 10 is disposed at the bottom edge of the support plate 7 , and a side plate of the U-shaped limiting plug-in 10 is inserted into the slot 601 .

[0034] Limiting vertical grooves 501 are provided on both sides of the guide vertical rod 5 , and limiting strips 602 are provided on the inner wall of the U-shaped frame 6 corresponding to the limiting vertical grooves 501 , and the limiting strips 602 are arranged in the limiting vertical grooves 501 .

[0035] A rubber cushion layer is arranged inside the U-shaped frame 6 .

[0036] A bending reinforcement plate 11 is arranged between the U-shaped limiting plug-in unit 10 and the bottom of the support plate 7 .

[0037] When chicken embryo tissue is obtained, first take the embryonated egg hatched to appropriate embryonic age (9-12 days), use the egg lighting lamp to check in the dark, if there are embryonated eggs with abundant blood vessels and embryonic body movement, it is shown that the embryonic body is well developed, and the air chamber and embryonic body position are marked with a colored pen, and afterwards the embryonated egg selected is used to clean the eggshell in the cleaning part 4 with warm water, and then wiped dry with 75% alcohol cotton balls;

[0038] Under aseptic conditions, embryo eggs are placed on the egg tray 9, and then the air pump is started to inflate the annular extrusion airbag 16. After the annular extrusion airbag 16 forms a slight extrusion on the egg tray 9, the air pump is stopped, and then the height of the lifting member is adjusted as needed. During the adjustment, the U-shaped frame 6 is held by hand, and the rotating shaft 12 is rotated so that the rubber projection 1201 does not contact the guide vertical rod 5. The height of the lifting U-shaped frame 6 is adjusted to a suitable height, and the rotating shaft 12 is rotated so that the rubber projection 1201 contacts the guide vertical rod 5, and the U-shaped frame 6 is limited by squeezing the rubber projection 1201. After the U-shaped frame 6 is in place, the air chamber is opened with scissors or tweezers in an aseptic manner, and the eggshell is removed along the edge of the air chamber. The shell membrane is torn off with ophthalmic tweezers to expose the chicken embryo, and the embryo head is gently picked up with elbow tweezers, and the embryo is taken out, and is placed in a sterile plate for dissection and sampling;

[0039] After the tissue sampling is completed, open the sealing cover, the gas in the air bag will flow out, and the embryo egg can be removed.

[0040] Technical features not described in the present invention can be achieved through or by adopting existing technologies, and will not be elaborated here. Of course, the above description is not a limitation of the present invention, and the present invention is not limited to the above examples. Changes, modifications, additions or substitutions made by ordinary technicians in this technical field within the essential scope of the present invention should also fall within the scope of protection of the present invention.

Claims

1. A tissue cell separation auxiliary device, characterized in that: It comprises a base, a lifting mechanism arranged on the base, a clamping seat is arranged on the lifting mechanism, and a cleaning part and an egg-illuminating lamp are also arranged on the base; The lifting mechanism comprises a guide vertical rod arranged on the base, a lifting member is arranged on the periphery of the guide vertical rod, and the clamping seat is arranged on the side of the lifting member; The clamping seat includes a support plate arranged on the lifting member, an egg tray is arranged on the support plate, an annular vertical plate is arranged on the periphery of the egg tray and the top surface of the support plate, an annular extrusion airbag is arranged on the inner side of the annular vertical plate, an air inlet pipe and an exhaust pipe are arranged on the side of the annular extrusion airbag, a sealing cover is arranged on the end of the exhaust pipe, an air pump is arranged on the base, and the air inlet pipe is connected to the air pump through a hose.

2. The tissue cell separation auxiliary device according to claim 1, characterized in that: The lifting member comprises a U-shaped frame arranged on the periphery of the guide vertical rod, the end surfaces of the side plates on both sides of the U-shaped frame are provided with wing plates, a rotating shaft is arranged between the wing plates on both sides, and the two ends of the rotating shaft are respectively penetrated through the wing plates on both sides to be provided with limited stop rings, and a U-shaped turning handle is arranged between the two ends of the rotating shaft; The outer surface of the rotating shaft is provided with rubber bumps.

3. The tissue cell separation auxiliary device according to claim 2, characterized in that: The front end of the U-shaped frame is provided with a slot penetrating up and down, the bottom edge of the support plate is provided with a U-shaped limiting plug-in, and a side plate on one side of the U-shaped limiting plug-in is inserted into the slot.

4. The tissue cell separation auxiliary device according to claim 3, characterized in that: Limiting vertical grooves are arranged on both sides of the guide vertical rod, and limiting strips are arranged on the inner wall of the U-shaped frame corresponding to the limiting vertical grooves, and the limiting strips are arranged in the limiting vertical grooves.

5. The tissue cell separation auxiliary device according to claim 4, characterized in that: A rubber cushion layer is arranged inside the U-shaped frame.

6. The tissue cell separation auxiliary device according to claim 5, characterized in that: A bending reinforcement plate is arranged between the U-shaped limiting plug-in and the bottom of the support plate.

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