Rapid protein precipitation device with filtering structure

Abstract

The utility model discloses a rapid protein precipitation device with a filtering structure, which relates to the technical field of protein precipitation and comprises a stirring barrel and a filtering box, a stirring shaft is rotatably sleeved in the stirring barrel, an installation ring and a plurality of stirring blades are respectively and fixedly sleeved on the outer wall of the stirring shaft, and the filtering box is arranged on the installation ring. Cleaning plates are fixedly connected to the outer walls of the two sides of the mounting ring, cleaning brushes are fixedly connected to the lower ends of the two cleaning plates, a flow guide ring fixedly sleeves the inner wall of the stirring barrel, an impurity filter screen is fixedly connected to the upper end of the flow guide ring, and a flow guide pipe fixedly sleeves one side of the lower end face of the stirring barrel. The lower end of the flow guide pipe penetrates through the filter box, the outer wall of the stirring barrel is fixedly sleeved with a heating barrel, one end of the heating barrel is fixedly sleeved with a steam access pipe, impurities in raw materials are filtered through an impurity filter screen, the filter screen can be prevented from being blocked by the impurities, and therefore the efficiency is higher when the filter screen is used for filtering protein.

Description

Technical Field

[0001] The utility model relates to the technical field of protein precipitation, in particular to a protein rapid precipitation device with a filtering structure. Background Art

[0002] Protein is the material basis of life, an organic macromolecule, the basic organic matter that constitutes cells, and the main bearer of life activities. There is no life without protein. Amino acids are the basic building blocks of protein. It is a substance closely related to life and various forms of life activities. Every cell and all important components in the body involve protein. Protein precipitation is the process in which the solute in the solution changes from liquid phase to solid phase and precipitates. The phenomenon of protein precipitation from solution is called protein precipitation. Common methods of protein precipitation include salting out, isoelectric precipitation, organic solvent precipitation, precipitation with alkaloid reagents and certain acids (such as trichloroacetic acid), etc.

[0003] Currently, protein precipitation generally involves pouring a protein precipitant and a salt solution into a container and stirring them. However, there is a lack of filtration of impurities before stirring, which results in clogging due to impurities during protein filtration, affecting the overall efficiency. Based on this, we propose an improved protein rapid precipitation device with a filtration structure. Utility Model Content

[0004] The main purpose of the utility model is to provide a protein rapid precipitation device with a filtering structure, which can effectively solve the technical problems in the background technology.

[0005] In order to achieve the above purpose, the technical solution adopted by the utility model is:

[0006] A protein rapid precipitation device with a filtering structure comprises a stirring barrel and a filter box, wherein a stirring shaft is rotatably sleeved in the stirring barrel, a mounting ring and a plurality of stirring blades are fixedly sleeved on the outer wall of the stirring shaft, cleaning plates are fixedly connected to the outer walls on both sides of the mounting ring, and cleaning brushes are fixedly connected to the lower ends of the two cleaning plates, a guide ring is fixedly sleeved on the inner wall of the stirring barrel, an impurity filter is fixedly connected to the upper end of the guide ring, a guide pipe is fixedly sleeved on one side of the lower end surface of the stirring barrel, and the lower end of the guide pipe passes through the filter box, a heating cylinder is fixedly sleeved on the outer wall of the stirring barrel, a steam access pipe is fixedly sleeved on one end of the heating cylinder, and a filter is slidably sleeved in the filter box.

[0007] As a further solution of the utility model, a plurality of support columns are fixedly connected between the mixing barrel and the filter box, guide rails are fixedly connected to the front and rear inner walls of the filter box, a mounting frame is slidably connected to the guide rails, and the filter screen is fixedly arranged in the mounting frame.

[0008] As a further solution of the utility model, a rectangular opening is opened on one side of the filter box, the installation frame is slidably sleeved in the rectangular opening, and sealing strips are fixedly provided on the upper and lower inner walls of the rectangular opening.

[0009] As a further solution of the utility model, the inner cavity height of the heating cylinder is greater than the vertical height between the uppermost stirring blade and the stirring barrel, and the upper horizontal height of the filter screen is less than the upper height of the mounting frame.

[0010] As a further solution of the utility model, the impurity filter is rotatably sleeved on the outer wall of the stirring shaft, the impurity filter is made of a conical structure, and the cleaning brush is arranged to fit the surface of the impurity filter.

[0011] As a further solution of the utility model, a feeding port is fixedly provided on the top plate of the mixing barrel, a sewage pipe is fixedly connected to one side of the mixing barrel, a liquid discharge pipe is fixedly connected to one side of the filter box, and solenoid valves are rotatably provided on the sewage pipe, the guide pipe and the liquid discharge pipe, and one end of the sewage pipe passes through a guide ring.

[0012] As a further solution of the utility model, a motor is fixedly provided on the upper end of the top plate of the mixing barrel, and the output end of the motor is fixedly connected to the upper end of the mixing shaft.

[0013] The beneficial effects of the utility model are as follows:

[0014] By filtering impurities in the raw materials through the impurity filter, it is possible to prevent the impurities from clogging the filter, thereby making it more efficient to filter protein through the filter, thereby accelerating the overall efficiency of the protein precipitation operation. At the same time, in conjunction with the heating cylinder sleeved at the outer end of the stirring barrel, the high-temperature steam provided by the steam heater can be connected through the steam access pipe to heat the stirring barrel, thereby further increasing the protein precipitation speed;

[0015] By setting up a conical impurity filter and rotatably setting a cleaning brush on the upper end thereof, it is convenient to use the stirring shaft to drive the installation ring to rotate later, so that the cleaning plate drives the cleaning brush to rotate to clean the surface of the impurity filter, and then the impurities are discharged through the drain pipe to achieve the cleaning of the filter, which is practical. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 This is a schematic diagram of the external structure of a protein rapid precipitation device with a filtering structure according to the utility model;

[0017] Figure 2 This is a schematic diagram of the internal structure of a protein rapid precipitation device with a filtering structure according to the utility model;

[0018] Figure 3This is a schematic diagram of the front cross-section structure of a protein rapid precipitation device with a filtering structure according to the utility model;

[0019] Figure 4 for Figure 3 A magnified view of the structure in the middle.

[0020] In the figure: 1. mixing barrel; 2. heating cylinder; 3. support column; 4. filter box; 5. feeding port; 6. guide ring; 7. impurity filter; 8. stirring shaft; 9. mounting ring; 10. cleaning plate; 11. cleaning brush; 12. stirring blade; 13. drain pipe; 14. steam inlet pipe; 15. guide pipe; 16. guide rail; 17. mounting frame; 18. filter; 19. drain pipe; 20. sealing strip. DETAILED DESCRIPTION

[0021] In order to make the technical means, creative features, objectives and effects achieved by the present invention easy to understand, the present invention is further described below in conjunction with specific implementation methods.

[0022] like Figure 1-4 As shown, a protein rapid precipitation device with a filtering structure comprises a stirring barrel 1 and a filter box 4, a stirring shaft 8 is rotatably sleeved in the stirring barrel 1, a mounting ring 9 and a plurality of stirring blades 12 are fixedly sleeved on the outer wall of the stirring shaft 8, cleaning plates 10 are fixedly connected to the outer walls on both sides of the mounting ring 9, and cleaning brushes 11 are fixedly connected to the lower ends of the two cleaning plates 10, a guide ring 6 is fixedly sleeved on the inner wall of the stirring barrel 1, an impurity filter screen 7 is fixedly connected to the upper end of the guide ring 6, a guide pipe 15 is fixedly sleeved on one side of the lower end surface of the stirring barrel 1, and the lower end of the guide pipe 15 passes through the filter box 4, a heating cylinder 2 is fixedly sleeved on the outer wall of the stirring barrel 1, a steam access pipe 14 is fixedly sleeved on one end of the heating cylinder 2, and a filter screen 18 is slidably sleeved in the filter box 4.

[0023] In this embodiment, a plurality of support columns 3 are fixedly connected between the mixing barrel 1 and the filter box 4, guide rails 16 are fixedly connected to the front and rear inner walls of the filter box 4, a mounting frame 17 is slidably connected to the guide rails 16, and a filter screen 18 is fixedly arranged in the mounting frame 17, and the filter screen 18 can be taken out by the mounting frame 17.

[0024] In this embodiment, a rectangular opening is provided on one side of the filter box 4, and the mounting frame 17 is slidably arranged in the rectangular opening. Sealing strips 20 are fixedly arranged on the upper and lower inner walls of the rectangular opening to seal the contact surface between the mounting frame 17 and the filter box 4.

[0025] In this embodiment, the inner cavity height of the heating cylinder 2 is greater than the vertical height between the uppermost stirring blade 12 and the stirring barrel 1 , and the upper horizontal height of the filter screen 18 is less than the upper height of the mounting frame 17 .

[0026] In this embodiment, the impurity filter screen 7 is rotatably sleeved on the outer wall of the stirring shaft 8. The impurity filter screen 7 is made of a conical structure to facilitate the diversion of impurities. The cleaning brush 11 is arranged in contact with the surface of the impurity filter screen 7, and the surface of the impurity filter screen 7 is cleaned by the cleaning brush 11.

[0027] In this embodiment, a feeding port 5 is fixedly provided on the top plate of the mixing barrel 1, a sewage pipe 13 is fixedly sleeved on one side of the mixing barrel 1, and a liquid discharge pipe 19 is fixedly sleeved on one side of the filter box 4. Solenoid valves are rotatably provided on the sewage pipe 13, the guide pipe 15 and the liquid discharge pipe 19. One end of the sewage pipe 13 passes through the guide ring 6 to facilitate the sewage pipe 13 to discharge impurities between the guide ring 6 and the impurity filter 7.

[0028] In this embodiment, a motor is fixedly provided on the upper end of the top plate of the stirring barrel 1, and the output end of the motor is fixedly connected to the upper end of the stirring shaft 8. The stirring shaft 8 is driven to rotate by the motor, and the solution can be stirred by the stirring blades 12.

[0029] It should be noted that the utility model is a protein rapid precipitation device with a filtering structure. When in use, the protein precipitant and the salt solution are poured into the stirring barrel 1 through the feeding port 5, and then the impurities are filtered through the impurity filter 7 and enter the bottom of the stirring barrel 1, and then the motor is started to drive the stirring shaft 8 to rotate, and then the mixed solution is stirred by the stirring blade 12. After the protein is precipitated, the guide pipe 15 is opened, the protein is filtered through the filter 18, and the mixed solution is discharged through the discharge pipe 19;

[0030] In the above process, when heating the mixed solution can increase the protein precipitation rate, the steam inlet pipe 14 can be connected to an external steam heater, and the heating steam can be introduced into the heating tube 2 to heat the stirring barrel 1, thereby heating the mixed solution. When the stirring shaft 8 rotates, the cleaning plate 10 will be driven to rotate synchronously through the mounting ring 9, and then the surface of the impurity filter screen 7 will be cleaned by the cleaning brush 11. Under the action of centrifugal force, the impurities flow into the groove of the guide ring 6, which is convenient for centralized discharge through the sewage pipe 13 at a later time.

[0031] The above shows and describes the basic principle and main features of the utility model and the advantages of the utility model. Those skilled in the art should understand that the utility model is not limited by the above embodiments. The above embodiments and descriptions are only for explaining the principle of the utility model. Without departing from the spirit and scope of the utility model, the utility model may have various changes and improvements, which fall within the scope of the utility model to be protected. The scope of protection claimed by the utility model is defined by the attached claims and their equivalents.

Claims

1. A protein rapid precipitation device with a filtering structure, comprising a stirring barrel (1) and a filtering box (4), characterized in that: A stirring shaft (8) is rotatably sleeved in the stirring barrel (1), and a mounting ring (9) and a plurality of stirring blades (12) are respectively fixedly sleeved on the outer wall of the stirring shaft (8), and cleaning plates (10) are fixedly connected to the outer walls on both sides of the mounting ring (9), and cleaning brushes (11) are fixedly connected to the lower ends of the two cleaning plates (10). A guide ring (6) is fixedly sleeved on the inner wall of the stirring barrel (1), and an impurity filter screen (7) is fixedly connected to the upper end of the guide ring (6). A guide pipe (15) is fixedly sleeved on one side of the lower end surface of the stirring barrel (1), and the lower end of the guide pipe (15) penetrates the filter box (4). A heating cylinder (2) is fixedly sleeved on the outer wall of the stirring barrel (1), and a steam inlet pipe (14) is fixedly sleeved on one end of the heating cylinder (2), and a filter screen (18) is slidably sleeved in the filter box (4).

2. The protein rapid precipitation device with a filtering structure according to claim 1, characterized in that: A plurality of support columns (3) are fixedly connected between the mixing barrel (1) and the filter box (4); guide rails (16) are fixedly connected to the front and rear inner walls of the filter box (4); a mounting frame (17) is slidably connected to the guide rails (16); and the filter screen (18) is fixedly arranged in the mounting frame (17).

3. A protein rapid precipitation device with a filtering structure according to claim 2, characterized in that: A rectangular opening is provided on one side of the filter box (4), and the mounting frame (17) is slidably sleeved in the rectangular opening. Sealing strips (20) are fixedly provided on the upper and lower inner walls of the rectangular opening.

4. The protein rapid precipitation device with a filtering structure according to claim 2, characterized in that: The inner cavity height of the heating cylinder (2) is greater than the vertical height between the uppermost stirring blade (12) and the stirring barrel (1), and the upper horizontal height of the filter screen (18) is less than the upper height of the mounting frame (17).

5. The protein rapid precipitation device with a filtering structure according to claim 1, characterized in that: The impurity filter screen (7) is rotatably sleeved on the outer wall of the stirring shaft (8); the impurity filter screen (7) is made of a conical structure; and the cleaning brush (11) is arranged in contact with the surface of the impurity filter screen (7).

6. The protein rapid precipitation device with a filtering structure according to claim 1, characterized in that: A feeding port (5) is fixedly provided on the top plate of the mixing barrel (1), a sewage discharge pipe (13) is fixedly sleeved on one side of the mixing barrel (1), and a liquid discharge pipe (19) is fixedly sleeved on one side of the filter box (4). Solenoid valves are rotatably provided on the sewage discharge pipe (13), the flow guide pipe (15) and the liquid discharge pipe (19), and one end of the sewage discharge pipe (13) passes through the flow guide ring (6).

7. The protein rapid precipitation device with a filtering structure according to claim 1, characterized in that: A motor is fixedly arranged on the upper end of the top plate of the stirring barrel (1), and the output end of the motor is fixedly connected to the upper end of the stirring shaft (8).

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