Batch resuscitation device for cell cryopreservation tubes
By designing cell frozen storage batch recovery devices, using the improvement device to automatically control the position and time of the frozen storage pipe in the water bath pot, solve the problem of inconvenient operation, pollution and recovery time control in the traditional recovery method, and achieve efficient and stable and stable stability Cell resuscitation and improvement of the automation and safety of experimental operations.
Patent Information
- Application Number
- CN202323242439.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2023-11-30
- Publication Date
- 2025-01-28
- Estimated Expiration
- 2033-11-30
AI Technical Summary
During cell culture, the traditional cell freezing duct resuscitation method has problems such as inconvenient operation, easy contamination and difficulty in controlling resuscitation time, resulting in slow cell growth or necrosis.
A type of recovery device is designed, including water bath pots, frozen storage tubes, and improved devices. By improving the position and time of the frozen storage tube in the water bath pot, it can ensure uniform recovery and reduce artificial operations.
The efficiency and stability of batch recovery of cell frozen custody is achieved, reducing the risk of cell pollution, ensuring the efficiency and stability of cell resuscitation, and improving the automation and security of experimental operations.
Smart Images
Figure CN222411538U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the field of osteoclast culture, and in particular to a batch recovery device for cell cryopreservation tubes. Background Art
[0002] In the process of cell culture, the general process of traditional recovery is: quickly put the cell cryopreservation tube into a 37°C water bath, shake it from time to time, completely melt it within 1-2 minutes, then remove the cells under sterile conditions, centrifuge and discard the supernatant, and then inoculate them into cell culture bottles or other consumables for observation and adjustment. In this process, if batch recovery is required, the experimenter needs to hold multiple cell cryopreservation tubes and shake them in a water bath for recovery. When holding the cryopreservation tubes, it is very easy to immerse the tube mouth of the cryopreservation tube in the water of the water bath, which increases the possibility of cell contamination. At the same time, due to the inconvenience of operation, the experimenter is very likely to control the time poorly, so that the recovery time is too long, which greatly reduces the number of active cells in the tube, causing the cells in the later culture to grow slowly or cause cell necrosis. Utility Model Content
[0003] In order to solve the above-mentioned problems, the utility model provides a cell cryopreservation tube batch resuscitation device, comprising a water bath, a cryopreservation tube basket and a lifting device, wherein the water bath is provided with a water bath pot mouth, and fixed plates are symmetrically provided on both sides of the water bath pot mouth, and the lifting device is fixed to the water bath pot mouth through the fixed plates, and the lifting device comprises a cylinder, an eccentric wheel and a lifting basket, and the fixed plates are provided with a fixing frame, and a cylinder is installed in the fixing frame, and a bracket is installed at the movable end of the cylinder, an L-shaped bracket is provided on one side of the fixing frame, and an eccentric wheel is installed above the L-shaped bracket, and the lifting basket comprises a bottom plate, a handle and a linkage rod, and a linkage rod on one side of the lifting basket is hooked with the eccentric shaft of the eccentric wheel through a connecting rod, and the cryopreservation tube basket comprises a handle, a hole plate and a plurality of groups of limit rods arranged below the hole plate, and the handle is fixed to the hole plate.
[0004] Preferably, the eccentric wheel position of the lifting device and the bracket position are at the same height.
[0005] Preferably, the bracket of the lifting device matches the linkage rod on the lifting basket, and the linkage rod can slide in the bracket.
[0006] Preferably, the upper end of the lifting basket handle is connected to the linkage rod, and the lower end is fixed to the bottom plate. The diameter of the bottom plate is smaller than the diameter of the water bath pot mouth. The bottom plate is provided with bottom through holes arranged in an array with equal spacing to facilitate water flow.
[0007] Preferably, the orifice plate of the cryotube basket is provided with tube holes arranged at equal intervals for placing the cryotubes, and the size of the tube holes is slightly smaller than the diameter of the cryotubes, so that the cryotubes can shake slightly in the tube holes.
[0008] Preferably, the limiting rod of the freezing tube basket fits into the limiting groove on the inner wall of the water bath pot mouth.
[0009] The beneficial effects of the utility model are as follows: the utility model has a reasonable structural design and can revive cell cryopreservation tubes in batches; the design of the basket facilitates the overall taking and placing of the cryopreservation tubes; by setting a lifting device and fixing the lysis time, the cell recovery efficiency and cell stability are ensured; the degree of automation is high and the safety during the experimental operation is ensured. BRIEF DESCRIPTION OF THE DRAWINGS
[0010] The present invention will be described by way of examples with reference to the accompanying drawings, in which:
[0011] Figure 1 It is a three-dimensional schematic diagram of the utility model;
[0012] Figure 2 It is a schematic diagram of the overall structure of the utility model;
[0013] Figure 3 It is the front view of the utility model;
[0014] Figure 4 It is a full cutaway schematic diagram of the utility model;
[0015] Figure 5 It is a top view of the utility model;
[0016] In the figure: 1. lifting device; 2. cryotube lifting basket; 3. water bath; 4. fixing plate; 5. connecting rod; 6. cryotube; 7. limiting block; 11. cylinder; 12. eccentric wheel; 13. lifting basket; 14. bracket; 15. L-shaped bracket; 16. bottom plate; 17. handle; 18. linkage rod; 19. eccentric shaft; 21. handle; 22. orifice plate; 23. limiting rod; 24. tube hole; 31. water bath pot mouth; 32. limiting groove; 41. fixing frame. DETAILED DESCRIPTION
[0017] All features disclosed in this specification, or steps in all methods or processes disclosed, except mutually exclusive features and / or steps, can be combined in any manner.
[0018] Any feature of this specification, unless otherwise specified, can be replaced by other equivalent or alternative features with similar purposes. That is, unless otherwise specified, each feature is only an example of a series of equivalent or similar features.
[0019] like Figure 1-3The cell cryopreservation tube batch resuscitation device shown in the figure includes a water bath 3, a cryopreservation tube basket 2 and a lifting device 1, wherein the water bath 3 is provided with a water bath pot mouth 31, and the water bath pot mouth 31 is symmetrically provided with fixing plates 4 on both sides, and the lifting device 1 is fixed to the water bath pot mouth 31 through the fixing plates 4, and the lifting device 1 includes a cylinder 11, an eccentric wheel 12 and a lifting basket 13, and the fixing plates 4 are provided with a fixing frame 41, and the fixing frame 41 is installed with the cylinder 11, and the movable end of the cylinder 11 is installed with a bracket 14, and one side of the fixing frame 41 is provided with an L-shaped bracket 15, and the upper part of the L-shaped bracket 15 is installed with the eccentric wheel 12, and the lifting basket 13 includes a bottom plate 16, a handle 17 and a linkage rod 18, and the linkage rod 18 on one side of the lifting basket 13 is connected with the eccentric shaft 19 of the eccentric wheel 12 through a connecting rod 5, as shown in FIG. Figure 4 As shown, the cylinder 11 drives the bracket 14 to move, thereby driving the lifting basket 13 to lift up through the linkage rod 18, so that the cryotube 6 is separated from the water bath environment.
[0020] like Figure 2 , 5 As shown, the bracket 14 matches the linkage rod 18 on the lifting basket 13, and the linkage rod 18 can slide in the bracket 14. The bracket limits the linkage rod of the lifting basket. The position of the bracket 14 and the position of the eccentric wheel 12 are at the same height. The linkage rod 18 on one side of the lifting basket 13 is connected with the eccentric shaft 19 of the eccentric wheel 12 through the connecting rod 5. When the cryopreservation tube 6 is thawed and revived, the motor drives the eccentric wheel 12 to rotate, thereby driving the linkage rod 18 to move, so that the lifting basket 13 moves left and right in the water bath 1.
[0021] like Figure 2 As shown, the upper end of the handle 17 of the lifting basket 13 is connected to the linkage rod 18, and the lower end is fixed on the bottom plate 16. The diameter of the bottom plate 16 is smaller than the diameter of the water bath pot mouth 31. The bottom plate 16 is provided with bottom through holes arranged in an array with equal spacing to facilitate water flow, which is beneficial to uniform heating of the cryopreservation tube 6.
[0022] like Figure 2 , 4As shown, the freezing tube basket 2 includes a handle 21, a perforated plate 22 and a plurality of groups of limiting rods 23 arranged below the perforated plate 22. The handle 21 is fixed to the perforated plate 22, and the limiting rods 23 are matched with the limiting grooves 32 on the inner wall of the water bath pot mouth 31. In this way, the freezing tube basket 2 can be fixed above the water surface of the pot mouth 31 to avoid the risk of contamination of the freezing tube 6 and facilitate the overall placement of the freezing tube 6. The perforated plate 22 is provided with tube holes 24 arranged at equal intervals for placing the freezing tubes. The size of the tube holes 24 is slightly smaller than the diameter of the freezing tube 6. The cap of the freezing tube is A limit block 7 is also provided, which enables the mouth of the cryopreservation tube to be above the orifice plate 22. The cryopreservation tube 6 can be slightly shaken in the tube hole 24. The rapid melting method used to revive cells can ensure that the extracellular crystals melt in a very short time, and avoid the damage to the cells caused by intracellular recrystallization caused by slow melting and water infiltration into the cells. Therefore, when the cryopreservation tube 6 is revived in batches, when the lifting basket 13 moves left and right in the 37°C water bath, the cryopreservation tube 6 is driven to shake slightly in the tube hole 24, replacing the manual shaking operation, so that the cells and tissues can quickly pass through the "ice crystal danger period".
[0023] In the specific implementation of the utility model, a PLC is also arranged on the lifting device, and a timer is installed on the PLC control panel. On the one hand, the PLC controls the motor to drive the lifting basket 13 to move left and right in the water bath 1 to realize the recovery of the cryopreserved tube 6. On the other hand, the lifting of the lifting basket 13 is set by the timer to fix the lysis time, thereby avoiding cell damage caused by too long recovery time. At the same time, the workload of the experimenter is greatly reduced, the work efficiency is improved, and the melting rate of the cell lines in each cryopreserved tube is basically the same, thereby ensuring the survival rate of the cells. At the same time, the setting of the PLC automates the entire recovery process, and the experimenter can stay away from the recovery device to avoid the threat of bursting of the cryopreserved tube 6.
[0024] During use, the water bath 1 is first maintained at a constant temperature of 37°C. The experimenter places the cryotube sample to be thawed in the tube hole 24 of the cryotube basket plate 22. The cryotube basket 2 is inserted into the limiting groove 32 of the water bath pot mouth 31 and fixed. At this time, the bottom of the cryotube 6 contacts the bottom plate 16 of the lifting basket 13. Figure 4As shown, the motor drives the eccentric wheel 12 to drive the lifting basket 13 to move back and forth in the water bath 1, thereby driving the cryotube 6 to gently shake back and forth in the tube hole 24 of the cryotube lifting basket 2 to accelerate melting. At the same time, the timer is set by the PLC. When the timer signal is sent to the PLC, the PLC stops the rotation of the eccentric wheel 12, and the cylinder 11 starts to move, driving the bracket 14 to drive the linkage rod 18 of the lifting basket 13 to lift. The linkage rod 18 on one side of the lifting basket 13, the connecting rod 5 and the eccentric shaft 19 are movably connected. When lifting, the linkage rod 18 drives the connecting rod 5 to disengage from the eccentric wheel 12, so that the cryotube 6 can be separated from the water bath environment to avoid too long resuscitation time. The cryotube 6 is provided with a limit block 7, and the experimenter takes the sample as a whole through the handle 21 of the cryotube lifting basket to proceed to the next operation step.
[0025] The utility model has a reasonable structural design and can revive cell cryopreservation tubes in batches. The design of the basket facilitates the overall taking and placing of the cryopreservation tubes. By setting a lifting device and fixing the lysis time, the cell recovery efficiency and cell stability are ensured. The degree of automation is high and the safety during the experimental operation is ensured.
[0026] The present invention is not limited to the above-mentioned specific implementation modes, but extends to any new features or any new combination disclosed in this specification, as well as any new method or process steps or any new combination disclosed.
Claims
1. A cell cryopreservation tube batch recovery device, characterized in that , comprising a water bath (3), a cryotube basket (2) and a lifting device (1), wherein the water bath (3) is provided with a water bath pot opening (31), and fixing plates (4) are symmetrically provided on both sides of the water bath pot opening (31), and the lifting device (1) is fixed to the water bath pot opening (31) via the fixing plates (4), and the lifting device (1) comprises a cylinder (11), an eccentric wheel (12) and a lifting basket (13), and the fixing plates (4) are provided with fixing frames (41), and the fixing frames (41) are provided with the cylinder (11), and the movable ends of the cylinder (11) are provided with brackets (11) 4), an L-shaped bracket (15) is arranged on one side of the fixing frame (41), an eccentric wheel (12) is installed above the L-shaped bracket (15), the lifting basket (13) comprises a bottom plate (16), a handle (17) and a linkage rod (18), the linkage rod (18) on one side of the lifting basket (13) is connected to the eccentric shaft (19) of the eccentric wheel (12) through a connecting rod (5), the freezing tube lifting basket (2) comprises a handle (21), a perforated plate (22) and a plurality of groups of limit rods (23) arranged below the perforated plate (22), and the handle (21) is fixed to the perforated plate (22).
2. A cell cryopreservation tube batch thawing device according to claim 1, characterized in that: The position of the eccentric wheel (12) of the lifting device (1) and the position of the bracket (14) are located at the same height.
3. A cell cryopreservation tube batch thawing device according to claim 1, characterized in that: The bracket (14) of the lifting device (1) matches the linkage rod (18) on the lifting basket (13), and the linkage rod (18) can slide in the bracket (14).
4. A cell cryopreservation tube batch thawing device according to claim 1, characterized in that: The upper end of the handle (17) of the lifting basket (13) is connected to the linkage rod (18), and the lower end is fixed to the bottom plate (16). The diameter of the bottom plate (16) is smaller than the diameter of the water bath pot mouth (31). The bottom plate (16) is provided with bottom through holes arranged in an array at equal intervals to facilitate water flow.
5. The cell cryopreservation tube batch thawing device according to claim 1, characterized in that: The perforated plate (22) of the cryotube basket (2) is provided with tube holes (24) arranged at equal intervals for placing the cryotubes. The size of the tube holes (24) is slightly smaller than the diameter of the cryotubes (6), so that the cryotubes (6) can be slightly shaken in the tube holes (24).
6. The cell cryopreservation tube batch thawing device according to claim 1, characterized in that: The limiting rod (23) of the freezing tube basket (2) fits into the limiting groove (32) on the inner wall of the water bath pot mouth (31).