Heparin extraction equipment

Abstract

The utility model provides heparin extraction equipment which comprises a base, an enzymolysis tank is mounted on one side of the surface of the base, and a pulping box, an electrolytic hot oil furnace, an elution tank, a settling tank, an alcohol recovery tank assembly, a first centrifugal pump, a first recovery box, a water softener and a second centrifugal pump are mounted on the surface of the base and located on the left side and the right side of the enzymolysis tank respectively; the adsorption column assembly is mounted on the conveying section of the second centrifugal pump; one side of the enzymolysis tank is connected with a first conveying pipe and a second conveying pipe, an operation panel is arranged on the surface of the base and located on one side of the enzymolysis tank, and one side of the first recycling box is connected with a second recycling box. The heparin extraction equipment provided by the utility model has the advantages that the equipment is miniaturized, production can be realized anytime and anywhere through electrification and water supply, a single-machine full-flow production mode is realized, input-output is realized, a stainless steel cutting pump is adopted for circularly pulping, the space is small, the cost is low, the efficiency is high, all structures of the equipment are mounted in a quick connection manner, and the equipment is convenient to mount, dismount and maintain.

Description

Technical Field

[0001] The utility model relates to the field of heparin extraction, in particular to a heparin extraction device. Background Art

[0002] Heparin was first discovered in the liver and got its name from it. It is a mucopolysaccharide sulfate ester composed of glucosamine, L-iduronide, N-acetylglucosamine and D-glucuronic acid alternately. It has an average molecular weight of 15KDa and is strongly acidic. It is also found in tissues such as the lungs, blood vessel walls, and intestinal mucosa. It is a natural anticoagulant in animals and naturally exists in mast cells. It is now mainly extracted from bovine lungs or pig small intestinal mucosa.

[0003] Heparin is the most important anticoagulant and antithrombotic biochemical drug, and currently it can only be extracted from some animal tissues and cannot be synthesized artificially. The existing extraction method extracts sodium heparin from the pig small intestinal mucosa, and the extraction equipment is produced in a modular manner in large factories.

[0004] Traditional heparin extraction uses a crusher to break heparin down. This process is suitable for large-scale automated production, but it has high costs and low operating rates for small-scale liberalized production.

[0005] Therefore, it is necessary to provide a heparin extraction device to solve the above technical problems. Utility Model Content

[0006] The utility model provides a heparin extraction device, which solves the problems of high cost, large floor space and difficult manufacturing of traditional crushers.

[0007] In order to solve the above technical problems, the utility model provides a heparin extraction device, comprising:

[0008] A base, an enzymolysis tank is installed on one side of the base surface, and a beating box, an electrolytic hot oil furnace, an elution tank, a precipitation tank, an alcohol recovery tank assembly, a first centrifugal pump, a first recovery tank, a water softener, and a second centrifugal pump are installed on the surface of the base and on the left and right sides of the enzymolysis tank respectively;

[0009] an adsorption column assembly, wherein the adsorption column assembly is installed in the delivery section of the second centrifugal pump;

[0010] One side of the enzymolysis tank is respectively connected to a first delivery pipe and a second delivery pipe, an operation panel is provided on the surface of the base and located on one side of the enzymolysis tank, and one side of the first recovery box is connected to a second recovery box.

[0011] Preferably, the adsorption column assembly is connected to the elution tank via a connecting pipe, and the delivery end of the first centrifugal pump is respectively connected to the elution tank and the beating box via a connecting pipe.

[0012] Preferably, the enzymolysis tank is connected to the adsorption column assembly, the elution tank and the alcohol recovery tank assembly respectively through connecting pipes.

[0013] Preferably, the alcohol recovery tank assembly is connected to the second recovery box via a connecting pipe.

[0014] Preferably, a stirring device is provided inside the enzymolysis tank, and the stirring device comprises a motor, and one end of the motor output shaft is connected to a threaded rod via a coupling.

[0015] Preferably, one end and one side of the surface of the threaded rod are respectively connected with a first stirring blade and a second stirring blade.

[0016] Preferably, a movable component is provided on the surface of the threaded rod, and the movable component includes a mounting tube, a threaded sleeve is connected to the surface of the threaded rod and located inside the mounting tube, movable blocks are connected on both sides of the threaded sleeve, and movable leaves are connected on both sides of the movable block.

[0017] Preferably, a movable through groove adapted to the movable block is provided on the surface of the mounting tube, and mounting frames are connected to both ends of the mounting tube, and the mounting frames are connected to the inner wall of the enzymolysis tank.

[0018] Preferably, a protective component is provided on the surface of the operating panel, and the protective component includes two slide grooves, the interiors of the two slide grooves are slidably connected with sliders, the surfaces of the two sliders are connected to connecting frames, and a protective cover is connected between the two connecting frames.

[0019] Preferably, a fixing piece is provided at one end of the sliding block, and a plurality of fixing holes matching with the fixing piece are formed on the inner wall of the sliding groove.

[0020] Compared with the related art, the heparin extraction device provided by the utility model has the following beneficial effects:

[0021] The utility model provides a heparin extraction device, which is miniaturized and can be produced anytime and anywhere as long as it is powered on and water is supplied. The single-machine full-process production mode realizes input and output, adopts a stainless steel cutting pump for circulating pulping, has a small space, low cost and high efficiency, and all structural installations of the equipment are quick-connected, which is convenient for installation, disassembly and maintenance. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 A schematic structural diagram of a first embodiment of a heparin extraction device provided by the utility model;

[0023] Figure 2 for Figure 1 The first perspective stereoscopic structural diagram of the entire device shown;

[0024] Figure 3 for Figure 1 A second perspective stereoscopic structural diagram of the overall device shown;

[0025] Figure 4 A schematic structural diagram of a second embodiment of a heparin extraction device provided by the utility model;

[0026] Figure 5 A schematic structural diagram of a third embodiment of a heparin extraction device provided by the utility model;

[0027] Figure 6 for Figure 5 An enlarged schematic diagram of part A is shown.

[0028] Numbers in the figure: 1, base, 2, enzymolysis tank, 3, beating box 3, 4, electrolytic hot oil furnace, 5, adsorption column assembly, 6, elution tank, 7, sedimentation tank, 8, alcohol recovery tank assembly, 9, first centrifugal pump, 10, second recovery box, 11, first recovery box, 12, water softener, 13, first delivery pipe, 14, second delivery pipe, 15, operation panel, 16, second centrifugal pump,

[0029] 17. stirring device, 171. motor, 172. threaded rod, 173. first stirring blade, 174. second stirring blade,

[0030] 18. movable assembly, 181. mounting cylinder, 182. threaded sleeve, 183. movable block, 184. movable leaf, 185. movable slot, 186. mounting frame,

[0031] 19. Protective assembly, 191. Slide groove, 192. Sliding block, 193. Connecting frame, 194. Protective cover, 195. Fixing part. DETAILED DESCRIPTION

[0032] The utility model is further described below in conjunction with the accompanying drawings and implementation modes.

[0033] First embodiment

[0034] Please refer to Figure 1 , Figure 2 and Figure 3 ,in, Figure 1 A schematic structural diagram of a first embodiment of a heparin extraction device provided by the utility model; Figure 2 for Figure 1 The first perspective stereoscopic structural diagram of the entire device shown; Figure 3 for Figure 1 A heparin extraction device comprises:

[0035] A base 1, an enzymolysis tank 2 is installed on one side of the surface of the base 1, and a beating box 33, an electrolytic hot oil furnace 4, an elution tank 6, a precipitation tank 7, an alcohol recovery tank assembly 8, a first centrifugal pump 9, a first recovery tank 11, a water softener 12 and a second centrifugal pump 16 are installed on the surface of the base 1 and on the left and right sides of the enzymolysis tank 2;

[0036] An adsorption column assembly 5, wherein the adsorption column assembly 5 is installed in the delivery section of the second centrifugal pump 16;

[0037] One side of the enzymolysis tank 2 is respectively connected to a first delivery pipe 13 and a second delivery pipe 14 , an operation panel 15 is provided on the surface of the base 1 and located on one side of the enzymolysis tank 2 , and one side of the first recovery box 11 is connected to a second recovery box 10 .

[0038] The adsorption column assembly 5 is connected to the elution tank 6 via a connecting pipe, and the delivery end of the first centrifugal pump 9 is respectively connected to the elution tank 6 and the beating box 33 via a connecting pipe.

[0039] The enzymolysis tank 2 is connected to the adsorption column assembly 5, the elution tank 6 and the alcohol recovery tank assembly 8 respectively through connecting pipes.

[0040] The alcohol recovery tank assembly 8 is connected to the second recovery box 10 via a connecting pipe.

[0041] The equipment is divided into five parts: a beating box 3, an enzymolysis tank 2, an elution tank 6, an electrolytic hot oil furnace 44 and an alcohol recovery tank assembly 8. The whole equipment is divided into six links: beating, filtration, enzymolysis, adsorption, elution and alcohol recovery. When the equipment is working, a part of soft water is first injected into the beating box 3 manually, and then the thawed pig small intestine is poured into the beating box 3, and the cutting pump is started. The cutting pump breaks the pig small intestine and then draws it into the enzymolysis tank 2, and the valve connecting the enzymolysis tank 2 and the beating box 3 is opened, so that the liquid in the enzymolysis tank 2 flows back to the beating box 3 again, thereby forming a circulating beating process. After observing that the small intestine is broken to a certain extent, it can be stopped, and the connecting valve is closed, and all the solution in the beating box 3 is drawn into the enzymolysis tank 2 to enter the next process.

[0042] Turn on the stirring motor of enzymolysis tank 2 to drive the blades to rotate, so that the liquid in the tank is always in motion, turn on the motor hot oil furnace to start heating the liquid, and at the same time add an appropriate amount of alkaline water to the enzymolysis tank 2. After the temperature rises to 35°C, the PH is controlled between 8 and 9.

[0043] After the temperature reaches 35°C, add protease and lipase, stir evenly, and let the motor hot oil furnace continue to heat up to 58°C. When the temperature reaches 58°C, test the pH every 0.5h (the pH cannot be lower than 8). When the temperature reaches 58°C, stop heating and hydrolyze for 3h until the small intestine is hydrolyzed into the shape of water (the hydrolysis is complete when there are no lumps). Start heating to 83°C 4h after the hydrolysis, stir at intervals, stop heating when the temperature reaches 83°C, add industrial salt, and start filtering.

[0044] During the first filtration, load a 100-mesh filter bag, turn on the self-priming pump, and let the liquid in the enzymolysis tank 2 flow into the pulping box 3 after passing through the filter bag. At the same time, turn on the cutting pump to pump the liquid into the enzymolysis tank 2 to form a circulating filtration effect. Similarly, use 200-mesh and 300-mesh filters for fine filtration. After filtration, the pH is controlled between 8.5 and 9.

[0045] After filtration, enter the adsorption process, add the resin to the adsorption column, check whether the valve switches are normal, keep the pressure relief valve flowing consistently, and after the liquid temperature drops to 65°C, turn on the stirring motor of the enzymolysis tank 2, switch the direction of the self-priming pump valve, and start cyclic adsorption. After the adsorption is completed, close the valve, and at the same time open the valve associated with the adsorption column and the elution tank 6, add salt solution to the elution tank 6 in advance, and start cyclic elution. After the elution is completed, add 95% concentration alcohol to the drug solution for precipitation. After the heparin sodium slurry is drained, it is dried and crushed, and packaged and stored to obtain the crude heparin.

[0046] Note: After the elution of the first adsorption column is completed, brine is added to the elution tank 6 to regenerate the resin, and at the same time, the upper and lower hoses are connected to the second resin column to form a continuous production mode.

[0047] The entire production process is controlled by PLC+touch screen, and all kinds of valves in the pipeline are electrically controlled, so that operators only need to feed materials during the production process, and other valve switching actions are automatically controlled by the system.

[0048] Compared with the related art, the heparin extraction device provided by the utility model has the following beneficial effects:

[0049] The utility model provides a heparin extraction device, which is miniaturized and can be produced anytime and anywhere as long as it is powered on and water is supplied. The single-machine full-process production mode realizes input and output, adopts a stainless steel cutting pump for circulating pulping, has a small space, low cost and high efficiency, and all structural installations of the equipment are quick-connected, which is convenient for installation, disassembly and maintenance.

[0050] Second embodiment

[0051] Please refer to Figure 4Based on the heparin extraction device provided in the first embodiment of the present application, the second embodiment of the present application provides another heparin extraction device. The second embodiment is only a preferred embodiment of the first embodiment, and the implementation of the second embodiment will not affect the independent implementation of the first embodiment.

[0052] Specifically, the difference of the heparin extraction equipment provided in the second embodiment of the present application is that, in a heparin extraction equipment, a stirring device 17 is arranged inside the enzymatic hydrolysis tank 42, and the stirring device 17 includes a motor 171, and one end of the output shaft of the motor 171 is connected to a threaded rod 172 through a coupling.

[0053] One end and one side of the surface of the threaded rod 172 are respectively connected to a first stirring blade 173 and a second stirring blade 174 .

[0054] The motor 171 is threadedly connected to the center position of the top of the enzymolysis tank 2 through bolts. The motor 171 is a servo motor. The motor 171 is powered by an external power supply. The threaded rod 172 is a reciprocating screw. The first stirring blade 173 and the second stirring blade 174 can be rotated in situ by the threaded rod 172. The mounting cylinder 181 with a movable through groove 185 is used in conjunction with the movable blade 184 with a movable block 183. The threaded rod 172 can drive the movable blade 184 to move vertically up and down to flip the medium.

[0055] A movable component 18 is provided on the surface of the threaded rod 172, and the movable component 18 includes a mounting tube 181. A threaded sleeve 182 is connected to the surface of the threaded rod 172 and located inside the mounting tube 181 through a threaded connection. Both sides of the threaded sleeve 182 are connected to movable blocks 183, and both sides of the movable block 183 are connected to movable leaves 184.

[0056] A movable through groove 185 adapted to the movable block 183 is formed on the surface of the mounting tube 181 , and mounting frames 186 are connected to both ends of the mounting tube 181 , and the mounting frames 186 are connected to the inner wall of the enzymolysis tank 2 .

[0057] The working principle of a heparin extraction device provided by the utility model is as follows:

[0058] During use, when the medium inside the enzymolysis tank 2 is mixed, first start the motor 171 to drive the threaded rod 172 to rotate. When the threaded rod 172 rotates, it drives the first stirring blade 173 and the second stirring blade 174 on both sides to stir the medium inside the enzymolysis tank 2. At the same time, when the threaded rod 172 rotates, it drives the threaded sleeve 182 on the surface to move up and down. When the threaded sleeve 182 moves up and down, it drives the movable blocks 183 on both sides to move inside the movable groove 185. When the movable block 183 moves, it drives the movable blade 184 at one end to move up and down to flip the medium in the enzymolysis tank 2.

[0059] Compared with the related art, the heparin extraction device provided by the utility model has the following beneficial effects:

[0060] The utility model provides a heparin extraction device, wherein a motor 171 with a threaded rod 172, a first stirring blade 173, a second stirring blade 174 and a movable component 18 are arranged inside an enzymolysis tank 4, and the cooperation between them can fully stir and mix the medium added into the enzymolysis tank 2.

[0061] Third embodiment

[0062] Please refer to Figure 5 and Figure 6 Based on the heparin extraction device provided in the first embodiment of the present application, the third embodiment of the present application provides another heparin extraction device. The third embodiment is only a preferred embodiment of the first embodiment, and the implementation of the third embodiment will not affect the independent implementation of the first embodiment.

[0063] Specifically, the difference of a heparin extraction device provided in the third embodiment of the present application is that, in a heparin extraction device, a protective component 19 is provided on the surface of the operating panel 15, and the protective component 19 includes two slide grooves 191, and the interiors of the two slide grooves 191 are slidably connected with sliders 192, and the surfaces of the two sliders 192 are connected with connecting frames 193, and a protective cover 194 is connected between the two connecting frames 193.

[0064] Two slide grooves 191 are symmetrically opened on both sides of the surface of the operating panel 15, and a limiting block is connected to one side of the slider 192. A limiting groove matched with the limiting block is opened on one side of the inner wall of the slide groove 191. The use of the limiting block and the limiting groove can prevent the slider 192 from falling off inside the slide groove 191. The use of the slide groove 191 and the slider 192 can facilitate the opening and closing of the protective cover 194.

[0065] A fixing piece 195 is disposed at one end of the sliding block 192 , and a plurality of fixing holes adapted to the fixing piece 195 are formed on the inner wall of the sliding groove 191 .

[0066] The working principle of a heparin extraction device provided by the utility model is as follows:

[0067] During use, when the operating panel 15 is not in use, first separate the fixing part 195 inside the slider 192 from the fixing hole on the inner wall of the slide groove 191. After the fixing part 195 is taken out, pull the protective cover 194 downward. When the protective cover 194 moves, the slider 192 is driven to move inside the slide groove 191 through the connecting frame 193. After the protective cover 194 and the operating panel 15 overlap for protection, use the fixing part 195 to connect the slider 192 to the fixing hole on the inner wall of the slide groove 191.

[0068] Compared with the related art, the heparin extraction device provided by the utility model has the following beneficial effects:

[0069] The utility model provides a heparin extraction device, wherein slide grooves 191, sliders 192, connecting frames 193 and protective covers 194 are arranged on both sides of the surface of an operation panel 15 for coordinated operation, so that the touch screen on the surface of the operation panel 15 can be protected when the operation panel 15 is not in use, thereby preventing the touch screen on the surface of the operation panel 15 from being damaged by external collision.

[0070] The above description is only an embodiment of the present invention, and does not limit the patent scope of the present invention. Any equivalent structure or equivalent process transformation made by using the contents of the specification and drawings of the present invention, or directly or indirectly applied in other related technical fields, are also included in the patent protection scope of the present invention.

Claims

1. A heparin extraction device, characterized in that: include: A base, an enzymolysis tank is installed on one side of the base surface, and a beating box, an electrolytic hot oil furnace, an elution tank, a precipitation tank, an alcohol recovery tank assembly, a first centrifugal pump, a first recovery tank, a water softener, and a second centrifugal pump are installed on the surface of the base and on the left and right sides of the enzymolysis tank respectively; an adsorption column assembly, wherein the adsorption column assembly is installed in the delivery section of the second centrifugal pump; One side of the enzymolysis tank is respectively connected to a first delivery pipe and a second delivery pipe, an operation panel is provided on the surface of the base and located on one side of the enzymolysis tank, and one side of the first recovery box is connected to a second recovery box.

2. The heparin extraction device according to claim 1, characterized in that: The adsorption column assembly is connected to the elution tank via a connecting pipe, and the delivery end of the first centrifugal pump is respectively connected to the elution tank and the beating box via a connecting pipe.

3. The heparin extraction device according to claim 1, characterized in that: The enzymolysis tank is connected to the adsorption column assembly, the elution tank and the alcohol recovery tank assembly respectively through connecting pipes.

4. The heparin extraction device according to claim 1, characterized in that: The alcohol recovery tank assembly is connected to the second recovery box via a connecting pipe.

5. The heparin extraction device according to claim 1, characterized in that: A stirring device is arranged inside the enzymolysis tank, and the stirring device comprises a motor, and one end of the motor output shaft is connected to a threaded rod through a coupling.

6. The heparin extraction device according to claim 5, characterized in that: One end of the threaded rod and one side of the surface are respectively connected with a first stirring blade and a second stirring blade.

7. The heparin extraction device according to claim 5, characterized in that: A movable component is arranged on the surface of the threaded rod, and the movable component includes a mounting tube. A threaded sleeve is connected to the surface of the threaded rod and located inside the mounting tube through a threaded connection. Both sides of the threaded sleeve are connected to movable blocks, and both sides of the movable block are connected to movable leaves.

8. The heparin extraction device according to claim 7, characterized in that: A movable through groove adapted to the movable block is provided on the surface of the installation cylinder, and both ends of the installation cylinder are connected with a mounting frame, and the mounting frame is connected to the inner wall of the enzymolysis tank.

9. The heparin extraction device according to claim 1, characterized in that: A protective component is provided on the surface of the operation panel, and the protective component includes two slide grooves, the interiors of the two slide grooves are slidably connected with sliders, the surfaces of the two sliders are connected with connecting frames, and a protective cover is connected between the two connecting frames.

10. The heparin extraction device according to claim 9, characterized in that: A fixing piece is arranged at one end of the sliding block, and a plurality of fixing holes matching with the fixing piece are arranged on the inner wall of the sliding groove.

Images