Nucleic acid extraction device for nucleic acid detection

Abstract

The nucleic acid extraction device comprises an extraction box, an opening and closing door is rotationally installed on one side of the front end face of the extraction box through a hinge, a sealing rubber ring is connected to the inner wall of the opening and closing door in a fusion mode, a feeding port is embedded in the upper end face of the extraction box, a rubber plug is inserted into an inner cavity of the feeding port, and a first handle is fixed to the upper end face of the extraction box. A refrigeration module is embedded in the rear end face of the extraction box, guide rails are fixed to the two sides of the inner wall of the extraction box correspondingly, sliding grooves are formed in the surfaces of the guide rails in a sleeving and inserting mode, and a drawing plate is fixed between the two sliding grooves. According to the device disclosed by the utility model, when a nucleic acid sample is extracted, the extracted sample can be directly fed into the extraction test tube to be stored from the feeding opening, and the sample storage operation is not required to be performed after the door is opened, so that the sample extraction process is more convenient, and the extraction test tube can be stably clamped in the insertion cylinder and is prevented from being broken due to mutual collision; in cooperation with a refrigeration module installed in the extraction box, the sample transportation can be safer.

Description

Technical Field

[0001] The utility model relates to the technical field of nucleic acid extraction, in particular to a nucleic acid extraction device for nucleic acid detection. Background Art

[0002] Nucleic acid extraction devices for nucleic acid testing are devices used to extract target nucleic acids, such as DNA and RNA, from samples. Extraction devices usually include sample processing modules, nucleic acid extraction modules, and waste liquid treatment modules. They can separate nucleic acids from samples through different chemical and physical methods for subsequent nucleic acid testing and analysis. Extraction transport boxes are containers for transporting and storing extraction devices and samples, which can facilitate nucleic acid extraction and transportation, ensuring high quality and accuracy of samples during the extraction process, which is very important for laboratory work such as disease diagnosis, virus detection, and gene analysis.

[0003] A nucleic acid extraction tube, a nucleic acid extraction kit and a nucleic acid extraction system shown in publication number CN219117433U, the nucleic acid extraction tube comprises: a tube body, and an upper sieve plate and a bottom sieve plate arranged in the tube body; the upper end of the tube body is provided with a positive and negative pressure port, and the lower end is provided with an extraction reagent through port, and the positive and negative pressure ports are connected to the extraction reagent through port; the tube body cavity between the upper sieve plate and the bottom sieve plate forms a nucleic acid extraction cavity, and a solid-phase nucleic acid extraction resin is arranged in the nucleic acid extraction cavity.

[0004] In the above device, although the operation process is in an open space and is susceptible to contamination, which avoids contamination between samples during the nucleic acid extraction process, the extraction test tubes are directly placed in a box for transportation. The test tubes collide with each other and are prone to breakage and sample loss. The device does not have the function of better preserving the extracted samples, and the sample extraction operation is cumbersome. Utility Model Content

[0005] The purpose of the utility model is to solve the disadvantages of the existing nucleic acid extraction device for nucleic acid detection, which directly puts the extraction test tube into the box for carrying, and the test tubes collide with each other and are prone to breakage and sample loss, and have no function of better preserving the extracted samples. At the same time, the sample extraction operation is cumbersome.

[0006] In order to achieve the above purpose, the utility model adopts the following technical solutions:

[0007] A nucleic acid extraction device for nucleic acid detection, comprising an extraction box, a front end face of the extraction box is rotatably mounted with an opening and closing door through a hinge, the inner wall of the opening and closing door is fused and connected with a sealing rubber ring, the upper end face of the extraction box is embedded with a feeding port, and the inner cavity of the feeding port is plugged with a rubber plug, the upper end face of the extraction box is fixed with a first handle, and the rear end face of the extraction box is embedded with a refrigeration module;

[0008] Guide rails are fixed on both sides of the inner wall of the extraction box, and slide grooves are inserted on the surface of the guide rails, a pull-out plate is fixed between the two slide grooves, and a second handle is fixed on the front end surface of the pull-out plate, a turntable is rotatably mounted on the upper end surface of the pull-out plate, and a plurality of insert tubes are fixed on the outer side of the turntable, reset springs are fixed on both sides of the inner wall of the insert tube, and a splint is fixed on the extended end of the reset spring, an extraction test tube is sleeved on the inner cavity of the insert tube, and a reduction motor is fixed on the lower end surface of the pull-out plate.

[0009] As a further description of the above technical solution:

[0010] The exhaust end of the refrigeration module is arranged in the inner cavity of the extraction box.

[0011] As a further description of the above technical solution:

[0012] The feeding port is arranged above the extraction test tube.

[0013] As a further description of the above technical solution:

[0014] The clamping plate is slidably connected to the surface of the extraction test tube, and an elastic telescopic structure is formed between the clamping plate and the inserting tube via a reset spring.

[0015] As a further description of the above technical solution:

[0016] The extraction test tube forms a clampable and fixed structure between the clamping plate and the inserting tube.

[0017] As a further description of the above technical solution:

[0018] The output end of the reduction motor passes through the pull-out plate and is transmission-connected to the turntable, so as to drive the turntable to rotate.

[0019] As a further description of the above technical solution:

[0020] The pull-out plate forms a slidable structure through the slide groove and the guide rail.

[0021] In summary, due to the adoption of the above technical solution, the beneficial effects of the utility model are:

[0022] In the utility model, when extracting nucleic acid samples, the device can directly feed the extracted samples into the extraction tubes for storage from the feeding port, without opening the closing door to perform the sample storage operation, making the sample extraction process more convenient, and the extraction tubes can be stably clamped in the insert tube to prevent mutual collision and breakage. In conjunction with the refrigeration module installed in the extraction box, the sample transportation can be safer, thereby solving the problems in the background technology. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1It is a three-dimensional structural schematic diagram of a nucleic acid extraction device for nucleic acid detection in the utility model;

[0024] Figure 2 This is a schematic diagram of the structure of a nucleic acid extraction device for nucleic acid detection in the utility model from a rear view;

[0025] Figure 3 This is a schematic diagram of the structure inside the extraction box in the utility model;

[0026] Figure 4 This is a schematic diagram of the structure of the extraction box in the utility model when viewed from above.

[0027] Legend:

[0028] 1. Extraction box; 2. Opening and closing door; 3. Sealing rubber ring; 4. Feeding port; 5. Rubber plug; 6. First handle; 7. Refrigeration module; 8. Guide rail; 9. Slide; 10. Pull-out plate; 11. Second handle; 12. Turntable; 13. Insertion cylinder; 14. Reset spring; 15. Clamp; 16. Extraction tube; 17. Reducer motor. DETAILED DESCRIPTION

[0029] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments of the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0030] Reference Figure 1-Figure 4 A nucleic acid extraction device for nucleic acid detection includes an extraction box 1, a front end face of the extraction box 1 is rotatably provided with an opening and closing door 2, an inner wall of the opening and closing door 2 is fused and connected with a sealing rubber ring 3, an upper end face of the extraction box 1 is embedded with a feeding port 4, and an inner cavity of the feeding port 4 is plugged with a rubber plug 5, a first handle 6 is fixed on the upper end face of the extraction box 1, and a refrigeration module 7 is embedded and installed on the rear end face of the extraction box 1;

[0031] Guide rails 8 are fixed on both sides of the inner wall of the extraction box 1, and slide grooves 9 are inserted and inserted on the surface of the guide rails 8. A pull-out plate 10 is fixed between the two slide grooves 9, and a second handle 11 is fixed on the front end surface of the pull-out plate 10. A turntable 12 is rotatably installed on the upper end surface of the pull-out plate 10, and multiple insert tubes 13 are fixed and embedded on the outer side of the turntable 12. Reset springs 14 are fixed on both sides of the inner wall of the insert tube 13, and a clamping plate 15 is fixed on the extended end of the reset spring 14. An extraction test tube 16 is sleeved in the inner cavity of the insert tube 13, and a reduction motor 17 is fixed on the lower end surface of the pull-out plate 10;

[0032] The extraction test tube 16 is manually placed into the inner cavity of the insert tube 13, and the extraction test tube 16 is clamped and stabilized by the clamping plate 15, and then the opening and closing door 2 is closed to keep the extraction box 1 sealed. When extracting nucleic acid, the rubber plug 5 is manually pulled out to expose the feeding port 4, and the staff feeds the nucleic acid sample from the feeding port 4 into the extraction test tube 16 below. After completing the storage of a single sample, the output end of the reduction motor 17 rotates, driving the turntable 12 to rotate, so that another extraction test tube 16 rotates to the bottom of the feeding port 4, and the second sample can be stored;

[0033] After the nucleic acid extraction and storage is completed, the rubber stopper 5 is inserted into the feeding port 4, and the low temperature in the extraction box 1 is maintained by the refrigeration module 7, so that the nucleic acid sample can be stored for a longer time;

[0034] When the sample needs to be tested, the opening and closing door 2 is opened, and the second handle 11 is manually pulled to slide the pull-out plate 10 on the surface of the guide rail 8 through the slide grooves 9 on both sides, and the pull-out plate 10 is pulled out of the extraction box 1, and the extraction test tube 16 is manually taken out of the insert 13 and placed in the instrument for testing.

[0035] Furthermore, the exhaust end of the refrigeration module 7 is arranged in the inner cavity of the extraction box 1.

[0036] Furthermore, the feeding port 4 is arranged above the extraction test tube 16 .

[0037] Furthermore, the clamping plate 15 is slidably connected to the surface of the extraction test tube 16 , and the clamping plate 15 forms an elastic telescopic structure with the inserting tube 13 via the return spring 14 .

[0038] Furthermore, the extraction tube 16 is clamped and fixed by the clamping plate 15 and the inserting tube 13 .

[0039] Furthermore, the output end of the reduction motor 17 is connected to the turntable 12 through the pull-out plate 10 for driving the turntable 12 to rotate.

[0040] Furthermore, the pull-out plate 10 forms a slidable structure through the slide groove 9 and the guide rail 8 .

[0041] Working principle: When in use, first manually put the extraction test tube 16 into the inner cavity of the insert tube 13, clamp the extraction test tube 16 with the clamp 15 to stabilize it, and then close the opening and closing door 2 to keep the extraction box 1 sealed. When extracting nucleic acid, manually pull out the rubber plug 5 to expose the feeding port 4. The staff will feed the nucleic acid sample from the feeding port 4 into the extraction test tube 16 below. After completing the storage of a single sample, the output end of the reduction motor 17 rotates, driving the turntable 12 to rotate, so that another extraction test tube 16 rotates to the bottom of the feeding port 4, and can be carried out. The second sample is stored. After the nucleic acid extraction and storage are completed, the rubber stopper 5 is inserted into the feeding port 4, and the low temperature in the extraction box 1 is maintained by the refrigeration module 7, so that the nucleic acid sample can be stored for a longer time. When the sample needs to be tested, the opening and closing door 2 is opened, and the second handle 11 is manually pulled to make the pull-out plate 10 slide on the surface of the guide rail 8 through the slide grooves 9 on both sides, and the pull-out plate 10 is pulled out of the extraction box 1, and the extraction test tube 16 is manually taken out of the insert 13 and placed in the instrument for testing. The working principle of the utility model is thus completed.

[0042] The above are only preferred specific implementation methods of the utility model, but the protection scope of the utility model is not limited to this. Any technician familiar with the technical field within the technical scope disclosed by the utility model, who makes equivalent replacements or changes based on the technical scheme and utility model concept of the utility model, should be covered by the protection scope of the utility model.

Claims

1. A nucleic acid extraction device for nucleic acid detection, comprising an extraction box (1), characterized in that: A door (2) is rotatably mounted on one side of the front end face of the extraction box (1) via a hinge, a sealing rubber ring (3) is fused to the inner wall of the door (2), a feeding port (4) is embedded in the upper end face of the extraction box (1), and a rubber plug (5) is inserted into the inner cavity of the feeding port (4), a first handle (6) is fixed on the upper end face of the extraction box (1), and a refrigeration module (7) is embedded in the rear end face of the extraction box (1); Guide rails (8) are fixed on both sides of the inner wall of the extraction box (1), and a slide groove (9) is inserted and inserted on the surface of the guide rail (8). A pull-out plate (10) is fixed between the two slide grooves (9), and a second handle (11) is fixed on the front end surface of the pull-out plate (10). A turntable (12) is rotatably mounted on the upper end surface of the pull-out plate (10), and a plurality of insert tubes (13) are embedded and fixed on the outer side of the turntable (12). Reset springs (14) are fixed on both sides of the inner wall of the insert tube (13), and a clamping plate (15) is fixed on the extended end of the reset spring (14). An extraction test tube (16) is sleeved in the inner cavity of the insert tube (13), and a reduction motor (17) is fixed on the lower end surface of the pull-out plate (10).

2. A nucleic acid extraction device for nucleic acid detection according to claim 1, characterized in that: The exhaust end of the refrigeration module (7) is arranged in the inner cavity of the extraction box (1).

3. A nucleic acid extraction device for nucleic acid detection according to claim 1, characterized in that: The feeding port (4) is arranged above the extraction test tube (16).

4. A nucleic acid extraction device for nucleic acid detection according to claim 1, characterized in that: The clamping plate (15) is slidably connected to the surface of the extraction test tube (16), and the clamping plate (15) forms an elastic telescopic structure with the inserting tube (13) via a reset spring (14).

5. A nucleic acid extraction device for nucleic acid detection according to claim 1, characterized in that: The extraction test tube (16) forms a clampable and fixed structure between the clamping plate (15) and the inserting tube (13).

6. A nucleic acid extraction device for nucleic acid detection according to claim 1, characterized in that: The output end of the reduction motor (17) passes through the pull-out plate (10) and is transmission-connected to the turntable (12), so as to drive the turntable (12) to rotate.

7. A nucleic acid extraction device for nucleic acid detection according to claim 1, characterized in that: The pull-out plate (10) forms a slidable structure with the guide rail (8) through the slide groove (9).

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