Embryo unfreezing culture plate
By designing an embryo thawing culture plate, the problems of low efficiency and high cost in existing embryo thawing operations have been solved, realizing an efficient and low-cost embryo thawing process, which is convenient for the promotion and application of embryo factories.
Patent Information
- Application Number
- CN202420619948.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-03-28
- Publication Date
- 2025-02-07
- Estimated Expiration
- 2034-03-28
AI Technical Summary
The lack of dedicated tools for embryo thawing in existing technologies means that thawing operations using culture dishes in the laboratory require constant focusing, which affects work efficiency and is costly, making it unsuitable for large-scale promotion in embryo factories.
An embryo thawing culture plate was designed, comprising a thawing tank and a resuscitation tank. The tanks are of the same depth, located on the same microscope focal plane, and are integrally formed with the culture plate. It is equipped with multiple solution tanks to facilitate aseptic handling and transfer of embryos and reduce costs.
It improves the efficiency of the embryo thawing process, reduces the number of times the microscope needs to be focused, lowers operating costs, and facilitates its widespread application in embryo factories.
Smart Images
Figure CN222434488U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of embryo thawing and resuscitation, in particular to an embryo thawing culture plate. Background Art
[0002] like Figure 1 In the prior art, a vitrification rod includes a handle 1, an inner rod 2, a rod 3, and an embryo marking point 4. The handle 1 is connected to one end of the inner rod 2, and the other end of the inner rod 2 is connected to the rod 3. The top of the rod 3 is provided with an embryo marking point 4. In the prior art, three liquids are used for thawing and resuscitation of embryo products: WS washing solution, TS thawing solution, and DS dilution solution. During thawing and resuscitation, 1 ml of TS thawing solution is aseptically dispensed on the thawing culture plate and placed in a 37°C incubator without CO2, or on a heating table for at least 30 minutes. The thawing culture plate is taken out and placed under the lens on the microscope stage. After the embryo is placed in the TS thawing solution, the embryo is left to stand for a period of time. At the same time, 1 drop of DS dilution solution is aseptically placed in the sterile embryo thawing culture plate. The embryo is then transferred to the DS and kept for a period of time. During this period, 2 50μl WS droplets are aseptically placed; then the embryo is transferred to the 2 50μl WS droplets in turn, and each is left to stand for a period of time. The embryo should expand to its original size in the WS. After thawing is completed, the embryo is transferred away and the next embryo operation is performed.
[0003] In actual work, there is a lack of special tools for embryo thawing. Experiments in the laboratory are all carried out in culture dishes. The volume of 1 ml of TS thawing solution is relatively large, and a separate container needs to be set up next to the culture dish. This container is separate from the culture dish, and the container containing the TS thawing solution and the bottom of the culture dish are not on the same focal plane of the microscope. When transferring embryos, constant focusing is required, which affects work efficiency. This cannot adapt to the large-scale promotion of embryo factories. Summary of the invention
[0004] The utility model aims to provide an embryo thawing culture plate to solve the problems raised in the above background technology.
[0005] An embryo thawing culture plate comprises a thawing culture plate body, characterized in that a thawing groove is provided at the bottom front end of the thawing culture plate body, a recovery groove is provided at the rear end of the thawing groove, a notch is provided on the left side and right side of the thawing culture plate body corresponding to the thawing groove position, and the notch depth is less than the thawing groove, the notch on the left side of the thawing culture plate body corresponds to the left thawing groove, and the notch on the right side of the thawing culture plate body corresponds to the right thawing groove. The embryo thawing culture plate is also provided with an upper cover, which can keep the embryo thawing culture plate in a sterile environment, which is beneficial to the protection of the embryo, and the depth of the thawing groove is equal to that of the recovery groove, so that the thawing groove and the bottom of the recovery groove can be on the same microscope focal plane, avoiding repeated focusing, improving work efficiency, and the thawing groove and the recovery groove are integrally formed with the embryo thawing culture plate, reducing costs and being easy to operate.
[0006] Furthermore, the thawing tank includes a left thawing tank and a right thawing tank, and the left thawing tank and the right thawing tank are staggered with each other.
[0007] Furthermore, the recovery tank has at least four columns, the left column is used for the DS dilution solution tank containing DS dilution solution, the middle two columns are used for the WS1 washing solution tank and the WS2 washing solution tank containing WS washing solution, and the right column is used for the culture tank containing culture medium containing 10% (v / v) protein.
[0008] Furthermore, the resuscitation tank has at least three columns, the left column is a DS dilution solution tank for containing DS dilution solution, and the right two columns are a WS1 washing solution tank and a WS2 washing solution tank for containing WS washing solution.
[0009] Furthermore, the thawing tank is marked with TS above, the front end of the DS dilution solution tank column is marked with DS, the front end of the WS1 washing solution tank column is marked with WS1, and the front end of the WS2 washing solution tank column is marked with WS2.
[0010] Furthermore, the left thawing tank and the right thawing tank are symmetrically arranged on the thawing culture plate, and the two are connected or separated.
[0011] Further, the resuscitation tank includes a left resuscitation tank and a right resuscitation tank, the left resuscitation tank has at least three columns, the left column is a DS dilution solution tank for DS dilution solution, the right two columns are WS1 washing solution tank and WS2 washing solution tank for WS washing solution, and the right resuscitation tank has at least three columns, the left column is a DS dilution solution tank for DS dilution solution, and the right two columns are WS1 washing solution tank and WS2 washing solution tank for WS washing solution. Further, the resuscitation tank can also be set to multiple rows, preferably 10 rows, so that more tanks can be used in the thawing culture plate, and a thawing culture plate can thaw more embryos in one batch than a culture dish.
[0012] Furthermore, the thawing groove and the resuscitation groove have the same depth, and the resuscitation groove is configured as a hemispherical groove, a cylindrical groove or a conical groove.
[0013] Furthermore, the capacity of the thawing tank is not less than 1 ml and is used to load the TS thawing solution, and the capacity of each resuscitation tank is not less than 50 μL.
[0014] Furthermore, the left side of the left thawing tank and the bottom of the left side notch are on the same inclined plane, and the right side of the right thawing tank and the bottom of the right side notch are on the same inclined plane. In this way, when the vitrification freezing carrier rod is placed into the thawing tank through the notch, the embryo marking point is located at the bottom of the thawing tank. The thawing tank and the recovery tank are integrally formed with the thawing culture plate body.
[0015] The embryo thawing and recovery method using this device is as follows:
[0016] S1: Add about 1 ml of TS thawing solution to the thawing tank, cover it with the lid, and place the embryo thawing culture plate in a sterile CO2 incubator at 37°C, or on a heating table for at least 30 minutes. ;
[0017] S2: Take out the embryo thawing culture plate, keep the room temperature at 22-27°C, take out the carrier straw from the liquid nitrogen, and put the carrier straw with the embryo into the thawing tank through the notch. During the operation, when the liquid nitrogen tank is on the right side of the operator, put the carrier straw into the right thawing tank through the notch on the right side. When the liquid nitrogen tank is on the left side of the operator, put the carrier straw into the left thawing tank through the notch on the left side. After the embryo falls into the thawing tank, take out the carrier straw and let the embryo stand in the thawing tank for a total of 1 minute. During the standing period, drop 50-100 μL of DS dilution solution into the DS dilution solution tank;
[0018] S3: transfer the embryos to the DS dilution solution tank and keep them for 4 minutes. Gently pipette the embryos once to ensure that they are completely washed in the DS dilution solution tank. During this period, drop 50-100 μL of WS washing solution into the WS1 washing solution tank and the WS2 washing solution tank.
[0019] S4: Transfer the embryos to the WS1 wash solution tank, then the WS2 wash solution tank, and let stand for 4 minutes each.
[0020] S5: After the embryo is thawed, there are two options for immediate transplantation: transfer the embryo to a pre-equilibrated "transplant" medium, or further culture the embryo by transferring it to a pre-equilibrated medium for 4 hours of recovery. After recovery, transfer the embryo to a medium containing 10% (v / v) protein until it reaches the desired developmental stage for transplantation.
[0021] When the next embryo is thawed, the TS thawing solution can still be used, and the used WS1 washing solution tank, WS2 washing solution tank and DS dilution solution tank are no longer used.
[0022] Compared with the prior art, the utility model has the following beneficial effects: the embryo thawing culture plate of the utility model, the thawing tank, the recovery tank and the thawing culture plate are integrally formed, and the operation is convenient; the left thawing tank and the right thawing tank are staggered with each other on the thawing culture plate, which is beneficial to reducing the size of the embryo thawing culture plate and reducing the cost; and the thawing tank and the recovery tank have the same depth and are both on the same focal plane of the microscope, which is convenient for the transfer of embryos, and the microscope does not need to be repeatedly focused, thereby improving work efficiency. These advantages combined together can be conveniently promoted and used in embryo factories. BRIEF DESCRIPTION OF THE DRAWINGS
[0023] Figure 1 It is a schematic diagram of the structure of a vitrification freezing rod in the prior art;
[0024] Figure 2 This is a schematic diagram of the three-dimensional structure of the embryo thawing culture plate in the first embodiment of the utility model;
[0025] Figure 3 for Figure 2 Schematic diagram of the top view of the embryo thawing culture plate;
[0026] Figure 4 It is a schematic diagram of the three-dimensional structure of the embryo thawing culture plate in the second embodiment of the present utility model.
[0027] Figure 5 for Figure 4 Schematic diagram of the top view of the embryo thawing culture plate.
[0028] Figure 6 for Figure 4 Schematic diagram of the three-dimensional structure of the upper cover of the embryo thawing culture plate.
[0029] In the figure: 1. handle; 2. inner rod; 3. carrier rod; 4. embryo marking point; 10. thawing culture plate body; 20. thawing tank; 21. left thawing tank; 22. right thawing tank; 30. recovery tank; 31. left recovery tank; 32. right recovery tank; 40. notch; 41. left side notch; 42. right side notch; 50. enclosure; 60. upper cover. DETAILED DESCRIPTION
[0030] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.
[0031] Example 1
[0032] like Figure 1 In the prior art, a vitrification rod includes a handle 1, an inner rod 2, a rod 3, and an embryo marking point 4. The handle 1 is connected to one end of the inner rod 2, and the other end of the inner rod 2 is connected to the rod 3. The top of the rod 3 is provided with an embryo marking point 4.
[0033] like Figure 2-3 The utility model discloses an embryo thawing culture plate, comprising a thawing culture plate body 10, a thawing groove 20 is arranged at the bottom front end of the thawing culture plate body 10, a resuscitation groove 30 is arranged at the rear end of the thawing groove 20, a notch 40 is arranged on the left side and right side of the thawing culture plate body 10 corresponding to the thawing groove position, and the depth of the notch 40 is less than the depth of the thawing groove 20, and the thawing groove 20 comprises a left thawing groove 21 and a right thawing groove 22, and the left thawing groove 21 and the right thawing groove 22 are staggered with each other. The left side notch 41 of the thawing culture plate body 10 corresponds to the left thawing groove 21, and the right side notch 42 of the thawing culture plate body 10 corresponds to the right thawing groove 22. Both the left side notch 41 and the right side notch 42 can be used to fix the vitrification freezing carrier rod. In specific work, in which notch the vitrification freezing carrier rod is placed can be determined according to the left and right sides of the liquid nitrogen tank relative to the operator. When the liquid nitrogen tank is located on the left side of the operator, it is placed in the left side notch 41, and when the liquid nitrogen tank is located on the right side of the operator, it is placed in the right side notch 42. This can facilitate operation and improve work efficiency.
[0034] Furthermore, the recovery tank 30 has at least three columns, the left column is a DS dilution solution tank for containing DS dilution solution, and the right two columns are a WS1 washing solution tank and a WS2 washing solution tank for containing WS washing solution.
[0035] Furthermore, TS is marked above the thawing tank 20, the front end of the DS dilution solution tank array is marked DS, the front end of the WS1 washing solution tank array is marked WS1, and the front end of the WS2 washing solution tank array is marked WS2.
[0036] Further, the resuscitation tank 20 includes a left resuscitation tank 21 and a right resuscitation tank 22. The left resuscitation tank 21 has at least three columns, one column on the left side is a DS dilution solution tank for DS dilution solution, and two columns on the right side are WS1 washing solution tank and WS2 washing solution tank for WS washing solution. The right resuscitation tank 22 has at least three columns, one column on the left side is a DS dilution solution tank for DS dilution solution, and two columns on the right side are WS1 washing solution tank and WS2 washing solution tank for WS washing solution. Further, the resuscitation tank 20 can be arranged in multiple rows, preferably 10 rows.
[0037] Further, the thawing groove 20 has the same depth as the resuscitation groove 30, and the resuscitation groove is configured as a hemispherical groove, a cylindrical groove or a conical groove, so that the thawing groove 20 and the resuscitation groove 30 can be on the same focal plane.
[0038] Furthermore, the capacity of the thawing tank is not less than 1 ml and is used to load the TS thawing solution, and the capacity of each resuscitation tank is not less than 50 μL.
[0039] Furthermore, the left side of the left thawing tank 21 and the bottom of the left side notch 41 are on the same inclined plane, and the right side of the right thawing tank 22 and the bottom of the right side notch 42 are on the same inclined plane. In this way, when the vitrification freezing carrier rod is placed into the thawing tank 20 through the notch, the embryo marking point is located at the bottom of the thawing tank.
[0040] Embodiment 2:
[0041] like Figure 4-5 , further improved on the basis of Example 1. The left thawing tank and the right thawing tank are combined into a thawing tank 20, and the thawing tank 20 is provided with a notch 40 at both ends. A recovery tank 30 is provided below the thawing tank 20. The recovery tank 30 has at least three columns, the left column is a DS dilution solution tank for DS dilution solution, and the right two columns are WS1 washing solution tank and WS2 washing solution tank for WS washing solution. Preferably, the recovery tank 30 has a total of nine columns, which are divided into three groups, one group of three columns. Within the group, the left column is a DS dilution solution tank for DS dilution solution, and the right two columns are WS1 washing solution tank and WS2 washing solution tank for WS washing solution. The thawing tank 20 and the recovery tank 30 are integrally formed with the embryo thawing culture plate. Preferably, the culture plate is surrounded by a fence 50, and the fence 50 is 1-2 mm wide and 5-10 mm high. Furthermore, in order to protect the embryo thawing culture plate in a sterile environment, the culture plate is further provided with an upper cover 60, which can protect the embryo thawing culture plate in a sterile environment.
[0042] Obviously, the above embodiments are merely examples for the purpose of clear explanation, and are not intended to limit the implementation methods. For those skilled in the art, other different forms of changes or modifications can be made based on the above description. It is not necessary and impossible to list all the implementation methods here. The obvious changes or modifications derived therefrom are still within the scope of protection of the invention of the utility model.
Claims
1. An embryo thawing culture plate, comprising a thawing culture plate body, characterized in that: A thawing groove is arranged at the front end of the bottom of the thawing culture plate body, and a recovery groove is arranged at the rear end of the thawing groove. Notches are arranged on the left and right sides of the thawing culture plate body corresponding to the positions of the thawing grooves, and the depth of the notches is smaller than the thawing grooves. The notch on the left side of the thawing culture plate body corresponds to the left thawing groove, and the notch on the right side of the thawing culture plate body corresponds to the right thawing groove, and the depths of the thawing groove and the recovery groove are equal.
2. The embryo thawing culture plate according to claim 1, characterized in that: The thawing tank comprises a left thawing tank and a right thawing tank, and the left thawing tank and the right thawing tank are staggered with each other.
3. The embryo thawing culture plate according to claim 1, characterized in that: The embryo thawing culture plate is also provided with an upper cover.
4. The embryo thawing culture plate according to claim 3, characterized in that: The thawing tank is marked with TS above, the front end of the DS dilution solution tank column is marked with DS, the front end of the WS1 washing solution tank column is marked with WS1, and the front end of the WS2 washing solution tank column is marked with WS2.
5. The embryo thawing culture plate according to claim 1, characterized in that: The resuscitation tank has at least three columns, the left column is a DS dilution solution tank for containing DS dilution solution, and the right two columns are a WS1 washing solution tank and a WS2 washing solution tank for containing WS washing solution.
6. The embryo thawing culture plate according to claim 1, characterized in that: The left thawing tank and the right thawing tank are symmetrically arranged on the thawing culture plate, and the two are connected or separated.
7. The embryo thawing culture plate according to claim 5, characterized in that: The recovery slots include a left recovery slot and a right recovery slot, the left recovery slot has at least three rows, and the right recovery slot has at least three rows.
8. The embryo thawing culture plate according to claim 1, characterized in that: The thawing groove has the same depth as the recovery groove, and the recovery groove is configured as a hemispherical groove, a cylindrical groove or a conical groove.
9. The embryo thawing culture plate according to claim 1, characterized in that: The capacity of the thawing tank shall not be less than 1 ml, and the capacity of each recovery tank shall not be less than 50 μL.