Improved cell culture dish with pore plate

Abstract

The utility model discloses an improved cell culture dish with a pore plate, which comprises a bottom plate and a transparent cover, a base plate is integrally arranged on the surface of the bottom plate, and the transparent cover is covered above the bottom plate on the outer ring of the base plate; a plurality of culture grooves are uniformly formed in the surface of the base plate, liquid adding holes are formed in the positions, corresponding to the culture grooves, of the transparent cover, the liquid adding holes penetrate through the transparent cover, two arched rubber films are transversely fixed to the same horizontal position below the inner walls of the liquid adding holes, and the rubber films are distributed in a staggered mode and are attached to each other. The rubber films which are mutually staggered and sealed are transversely arranged in the liquid adding hole, a dropper can penetrate through the rubber films to drop a reagent during liquid adding, the rubber films are tightly attached to the outer surface of the dropper in the process to keep a certain sealing effect, and after the dropper is drawn away, the rubber films are staggered and contacted and have elasticity, so that the dropper is prevented from falling off. Therefore, cell exposure before and after liquid adding is reduced, and bacterial pollution is avoided.

Description

Technical Field

[0001] The utility model relates to an improved cell culture dish with a hole plate, belonging to the technical field of cell culture dishes. Background Art

[0002] Cell culture dishes are used for cell culture in laboratories. When nutrient solution or required reagents need to be added during the culture, the upper cover of the culture dish needs to be opened and added dropwise. However, when the upper cover is removed, the cells in the culture dish will be exposed for too long, which is prone to bacterial contamination and inconvenient for adding liquid.

[0003] For example, Chinese patent application CN219470043U discloses a cell culture dish with a perforated plate for easy liquid addition, which can avoid the problem of nutrient solution splashing during the addition process by providing a liquid injection cylinder. However, in the above solution, liquid addition requires opening the cover plate and using the liquid injection cylinder for multiple times, which may cause the cells in the culture dish to be exposed for too long during use, and the steps of using the liquid injection cylinder multiple times are relatively cumbersome.

[0004] Therefore, it is obviously necessary to propose an improved cell culture dish with well plates. Utility Model Content

[0005] The utility model aims to provide an improved cell culture dish with a perforated plate, wherein a liquid adding hole is provided on the surface of a transparent cover above a culture tank, and rubber membranes which are sealed and staggered with each other are arranged transversely inside the liquid adding hole, so that a dropper can pass through the rubber membrane to drop reagents when adding liquid, and during this process, the rubber membrane is tightly attached to the outer surface of the dropper to maintain a certain sealing effect, thereby reducing cell exposure during liquid addition and avoiding bacterial contamination, facilitating liquid addition operations, and solving the problems raised in the background technology.

[0006] In order to achieve the above-mentioned invention object, the technical solution adopted by the utility model is: an improved cell culture dish with a perforated plate, including a bottom plate and a transparent cover, the surface of the bottom plate is integrally provided with a base plate, and the transparent cover is placed above the bottom plate on the outer circle of the base plate; a plurality of culture grooves are evenly opened on the surface of the base plate, and the transparent cover is provided with liquid adding holes at positions corresponding to the culture grooves, and the liquid adding holes penetrate the transparent cover, and two rubber membranes are horizontally fixed at the same horizontal position below the inner wall of the liquid adding hole, and the rubber membranes are arched, and the rubber membranes are staggered and in contact with each other.

[0007] In a preferred technical solution, a protrusion is provided on the outer periphery of the rubber membrane, and an annular groove is provided on the inner wall of the liquid adding hole, and the protrusion is embedded in the groove on the inner wall of the liquid adding hole.

[0008] In a preferred technical solution, a rubber plug is further provided for use with the liquid adding hole, and the rubber plug is used to seal the liquid adding hole.

[0009] In a further technical solution, an annular sealing ring is provided on the outer ring of the rubber stopper, and the annular sealing ring contacts the inner surface of the liquid adding hole.

[0010] In a further technical solution, a resist is integrally connected to the upper part of the rubber stopper, the diameter of the resist is larger than the diameter of the liquid adding hole, and the resist contacts the surface of the transparent cover of the outer circle of the liquid adding hole.

[0011] Due to the application of the above technical solution, the utility model has the following advantages compared with the prior art:

[0012] 1. The utility model has rubber membranes that are staggered and sealed in a transverse manner inside the liquid adding hole. When adding liquid, the dropper can pass through the rubber membrane to drop the reagent. During this process, the rubber membrane is tightly attached to the outer surface of the dropper to maintain a certain sealing effect. When the dropper is withdrawn, the rubber membrane will immediately reset and contact the sealed opening structure because of its staggered contact and elasticity, thereby reducing cell exposure before and after adding liquid, avoiding bacterial contamination, and facilitating the liquid adding operation.

[0013] 2. The utility model can seal the liquid adding hole by setting a rubber plug, completely sealing the liquid adding hole during the culture process to avoid the influence of liquid leakage at the rubber membrane. The cooperation between the rubber plug, the liquid adding hole and the rubber membrane further increases the sealing performance inside the culture dish. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 The utility model is a schematic diagram of the overall structure of the improved cell culture dish with a hole plate.

[0015] Figure 2 This is a schematic diagram of the structure of the bottom plate and the surface above it of the improved cell culture dish with holes in the utility model.

[0016] Figure 3 The utility model is a schematic diagram of the structure of the liquid adding hole of the improved perforated plate cell culture dish.

[0017] Among them: 1. bottom plate; 11. substrate; 111. culture tank; 2. transparent cover; 3. liquid adding hole; 31. rubber membrane; 311. protrusion; 4. rubber plug; 41. stopper; 42. sealing ring. DETAILED DESCRIPTION

[0018] The utility model is further described below in conjunction with the accompanying drawings and embodiments: Embodiment 1

[0019] See also Figure 1~Figure 2As shown, an improved cell culture dish with a perforated plate includes a bottom plate 1 and a transparent cover 2. The surface of the bottom plate 1 is provided with a base plate 11 as a whole. The base plate 11 is located in the middle of the surface of the bottom plate 1. Six culture grooves 111 are evenly opened on the surface of the base plate 11. Cells and culture fluid can be added to the culture grooves 111. Then the transparent cover 2 is placed on the bottom plate 1 on the outer circle of the base plate 11 to carry out cell culture. The outer diameter of the transparent cover 2 is the same as the outer diameter of the bottom plate 1.

[0020] The transparent cover 2 is provided with liquid adding holes 3 at positions corresponding to the culture tanks 111. The sizes of all the liquid adding holes 3 are the same and the liquid adding holes 3 pass through the transparent cover 2. The inside of the liquid adding holes 3 is plugged with a rubber plug 4. The outer ring of the rubber plug 4 is integrally connected and fixed with an annular sealing ring 42. The sealing ring 42 abuts against the inner surface of the liquid adding hole 3, so that the liquid adding hole 3 is sealed with the rubber plug 4. The top of the rubber plug 4 is integrally connected with a resist piece 41. The resist piece 41 abuts against the surface of the transparent cover 2 at the outer ring of the liquid adding hole 3. The rubber plug 4 can be removed by pulling it upwards through the resist piece 41, and the rubber plug 4 can be prevented from slipping into the liquid adding hole 3.

[0021] See also Figure 3 As shown, two rubber membranes 31 are transversely fixed at the same horizontal position below the inner wall of each liquid adding hole 3, and the arc-shaped edge of the outer periphery of the rubber membrane 31 is provided with a protrusion 311, which is embedded in the annular groove of the inner wall of the liquid adding hole 3 to achieve stable fixation of the rubber membrane 31. The rubber membrane is arched, and the shape of the arch is larger than the semicircular shape. The edges of the rubber membranes 31 on both sides are staggered and in contact with each other, so as to achieve the blocking of the liquid adding hole 3. When liquid needs to be added, the rubber plug 4 can be removed first, and the dropper can be inserted into the liquid adding hole 3, and the rubber membrane 31 can be pushed open on both sides and placed above the culture tank 111. Then the liquid can be dripped. The rubber membrane 31 in contact with the outer surface of the dropper can maintain the sealing effect during liquid addition and avoid bacterial contamination caused by cell exposure.

[0022] When in use, the transparent cover 2 can be directly removed, and cells and culture solutions can be added to different culture tanks 111, and then the transparent cover 2 can be put on to carry out cell culture work. During this process, the real-time situation in each culture tank 111 can be conveniently observed through the transparent cover 2. When reagents or nutrient solutions need to be added, the transparent cover 2 is kept placed on the top of the bottom plate 1, the push piece 41 is pulled upward and the rubber plug 4 is pulled out from the liquid adding hole 3, and then the bottom of the dropper is inserted into the liquid adding hole 3, the rubber membrane 31 is pushed open and passed between the rubber membranes 31 on both sides, and then extended to the bottom of the transparent cover 2 and to the top position of the corresponding culture tank 111, and then the dropper is squeezed to add the reagents or nutrient solutions to be added into the culture tank 111, and then the dropper is removed and the rubber plug 4 is plugged again. In this process, a good sealing effect can be maintained to avoid liquid splashing, reduce the contact between cells and air, and avoid bacterial contamination.

Claims

1. An improved cell culture dish with a well plate, comprising a bottom plate (1) and a transparent cover (2), characterized in that: The surface of the bottom plate (1) is integrally provided with a base plate (11), and the transparent cover (2) is placed above the bottom plate (1) on the outer circle of the base plate (11); The surface of the substrate (11) is evenly provided with a plurality of culture grooves (111); the transparent cover (2) is provided with liquid adding holes (3) at positions corresponding to the culture grooves (111); the liquid adding holes (3) penetrate the transparent cover (2); two rubber membranes (31) are transversely fixed at the same horizontal position below the inner wall of the liquid adding holes (3); the rubber membranes (31) are arched, and the rubber membranes (31) are staggered and in contact with each other.

2. The improved cell culture dish with a hole plate according to claim 1, characterized in that: A protrusion (311) is provided on the outer periphery of the rubber membrane (31), an annular groove is provided on the inner wall of the liquid adding hole (3), and the protrusion (311) is embedded in the groove on the inner wall of the liquid adding hole (3).

3. The improved cell culture dish with a well plate according to claim 1, characterized in that: A rubber plug (4) is also provided for use with the liquid adding hole (3), and the rubber plug (4) is used to seal the liquid adding hole (3).

4. The improved cell culture dish with a well plate according to claim 3, characterized in that: The outer ring of the rubber stopper (4) is provided with an annular sealing ring (42), and the annular sealing ring (42) contacts the inner surface of the liquid adding hole (3).

5. The improved cell culture dish with a hole plate according to claim 3, characterized in that: A resist piece (41) is integrally connected to the upper portion of the rubber stopper (4); the diameter of the resist piece (41) is larger than the diameter of the liquid adding hole (3); and the resist piece (41) contacts the surface of the transparent cover (2) on the outer ring of the liquid adding hole (3).

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