Pretreatment device for organ-like embedding
By designing the rolling mechanism and limiting mechanism in the pretreatment device for organoid embedding, the problems of low dehydration efficiency and insufficient applicability are solved, and a more efficient dehydration process and wider applicability are achieved.
Patent Information
- Application Number
- CN202421314247.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-06-11
- Publication Date
- 2025-02-07
- Estimated Expiration
- Not applicable · inactive patent
AI Technical Summary
The existing pretreatment device for organoid embedding cannot rotate the angle during the dehydration process, resulting in incomplete contact between the agarose block and ethanol, insufficient dehydration efficiency, and insufficient device applicability, and inability to adapt to cylinders of different diameters.
A pretreatment device including a rolling mechanism and a limiting mechanism is designed. The tumbling mechanism uses the combination of sliding components, damping oil and rolling cylinder to make the dehydration box roll during the dehydration process, increasing the contact area between the agarose block and ethanol. The limiting mechanism adjusts the overall length through the telescopic arm to adapt to cylinders of different diameters.
The agarose blocks are rolled in the dehydration box through the rolling mechanism, which improves the dehydration efficiency; through the adjustment of the limiting mechanism, the applicability of the device is improved and can be adapted to cylinders of different diameters.
Smart Images
Figure CN222439241U_ABST
Abstract
Description
Technical Field
[0001] The utility model belongs to the technical field of experimental equipment, and specifically relates to a pretreatment device for embedding an organoid. Background Art
[0002] Organoids are derived from the body's own tissues or stem cells. They are three-dimensional structural models with original tissues and organs formed through in vitro 3D culture. Organoids need to be pretreated before embedding, including dehydration and transparency. Dehydration refers to taking out the solidified agarose block containing organoid precipitation and dehydrating it in gradient ethanol. The pretreatment device for organoid embedding used for dehydration is mainly composed of a horizontal plate, a vertical plate and a perforated dehydration box. When in use, the agarose block containing organoids is placed in the box body, the dehydration box is fixedly installed in the vertical plate, the vertical plate is installed at the bottom of the horizontal plate, and the horizontal plate is placed on the cylinder filled with ethanol, so that the dehydration box is immersed in ethanol, so that ethanol can dehydrate the agarose block containing organoids.
[0003] The dehydration box of the existing pretreatment device for organoid embedding is generally fixedly connected to the vertical plate and cannot be rotated during the dehydration process. The agarose block containing the organoid is in a static state in the dehydration box, and one side of the agarose block is likely to always fit with the inner wall of the dehydration box, resulting in incomplete contact between this part of the agarose block and ethanol, insufficient dehydration efficiency, and the length of the horizontal plate is generally fixed and cannot be adjusted. When the length of the horizontal plate is insufficient, it cannot be placed on the cylinder filled with ethanol, and it is difficult to adapt to cylinders of different calibers, and the applicability is insufficient. Utility Model Content
[0004] The utility model aims to provide a pretreatment device for organoid embedding, which can make an agarose block containing organoids tumble in a dehydration box during the dehydration process through a tumbling mechanism, thereby reducing the incomplete contact between a conventional static agarose block and ethanol, improving the dehydration efficiency, and can adjust the overall length of the limiting mechanism through a telescopic arm so as to be mounted on cylinders of different calibers, thereby improving applicability.
[0005] The technical solution adopted by the utility model is as follows:
[0006] A pretreatment device for organoid embedding, comprising a housing, and further comprising:
[0007] A rolling mechanism, which is arranged inside the housing and includes a sliding assembly, damping oil and a rolling cylinder;
[0008] A limiting mechanism, wherein two limiting mechanisms are provided and are respectively located at two ends of the top of the shell, and the limiting mechanism comprises a telescopic arm;
[0009] A dehydration box, wherein two dehydration boxes are provided and are respectively fixedly connected to the two ends of the rolling drum;
[0010] The sliding assembly is hindered by the damping oil and moves slowly downward, so that the rolling cylinder drives the dehydration box to roll, and the telescopic arm slides along the top of the shell to adjust the overall length of the limiting mechanism.
[0011] The tumbling mechanism also includes a sealed box fixedly arranged on the top of the shell, the sliding assembly and the damping oil are both arranged inside the sealed box, the sliding assembly is slidably connected to the sealed box in the vertical direction, the upper end of the sliding assembly is fixedly connected to a counterweight box, the lower end of the sliding assembly is fixedly connected to a rack, the rolling cylinder is rotatably connected to the inner walls on both sides of the sealed box, the rolling cylinder is fixedly connected to a gear, and the gear is meshed with the rack.
[0012] The sliding assembly includes a slider slidably connected to the inside of a sealing box, an oil drain hole and a plurality of first oil holes are circumferentially opened inside the slider, the aperture sizes of the plurality of first oil holes are different, a stopper is rotatably installed on the top of the slider, the stopper is rotatably connected to the sealing box, and a second oil hole is opened inside the stopper.
[0013] The counterweight box comprises a box body fixedly connected to the upper end of the sliding block, and a counterweight hole is opened on the top of the box body.
[0014] The limiting mechanism also includes a slide groove fixedly connected to the top of the shell, and the telescopic arm is slidably installed on the inner side of the slide groove.
[0015] A plurality of first limiting holes are provided inside the telescopic arm, a second limiting hole is provided inside the slide slot, and the second limiting hole is slidably connected to the first limiting hole through a limiting pin.
[0016] The technical effects achieved by the utility model are:
[0017] The tumbling mechanism provided in the utility model can make the agarose block containing organoids tumble in the dehydration box during the dehydration process. The counterweight box drives the sliding component to move downward under the action of its own gravity. The sliding component drives the rolling drum to rotate through the rack and the gear, and the rolling drum drives the dehydration box to rotate, so that the agarose block containing organoids inside the dehydration box rolls during the dehydration process, reducing the incomplete contact between the traditional static agarose block and ethanol, and improving the dehydration efficiency. During the downward movement of the sliding component, the damping oil under the sliding component moves through the sliding component to the top of the sliding component. Since the movement speed of the damping oil is relatively slow, the damping oil still located under the sliding component hinders the downward movement of the sliding component, slows down the downward movement speed of the sliding component, and prolongs the rolling time of the agarose block to adapt to the time-consuming dehydration process.
[0018] The limiting mechanism provided in the utility model can adjust the overall length through the telescopic arm so as to be mounted on cylinders of different calibers. After the telescopic arm slides on the top of the shell, the distance between the ends of the telescopic arm increases or decreases, thereby adjusting the overall length of the limiting mechanism to adapt to cylinders of different calibers and improve applicability. BRIEF DESCRIPTION OF THE DRAWINGS
[0019] Figure 1 It is a schematic diagram of the overall structure of the utility model;
[0020] Figure 2 It is a cross-sectional schematic diagram of the entire utility model;
[0021] Figure 3 This utility model Figure 2 The enlarged schematic diagram of point A in the middle;
[0022] Figure 4 It is an exploded schematic diagram of the sliding component in the utility model.
[0023] In the accompanying drawings, the components represented by the reference numerals are listed as follows:
[0024] 10. Shell; 20. Tumbling mechanism; 21. Sliding assembly; 22. Damping oil; 23. Rolling cylinder; 231. Gear; 24. Sealing box; 25. Counterweight box; 251. Box body; 252. Counterweight hole; 26. Rack; 27. Sliding block; 271. Oil drain hole; 272. First oil hole; 28. Stop block; 281. Second oil hole; 29. Cover plate; 30. Limiting mechanism; 31. Telescopic arm; 311. First limiting hole; 32. Slide; 321. Second limiting hole; 33. Limiting pin; 40. Dehydration box. DETAILED DESCRIPTION
[0025] In order to make the purpose and advantages of the utility model more clear, the utility model is specifically described in combination with the following embodiments. It should be understood that the following text is only used to describe one or several specific implementation methods of the utility model, and does not strictly limit the scope of protection of the specific request of the utility model.
[0026] like Figure 1 and Figure 2 As shown, a pretreatment device for organoid embedding includes a housing 10, and further includes:
[0027] A rolling mechanism 20, which is disposed inside the housing 10 and includes a sliding assembly 21, damping oil 22 and a rolling cylinder 23;
[0028] A limiting mechanism 30, wherein two limiting mechanisms 30 are provided and are respectively located at two ends of the top of the housing 10, and the limiting mechanism 30 includes a telescopic arm 31;
[0029] Dehydration boxes 40, two of which are provided and fixedly connected to both ends of the rolling drum 23;
[0030] The sliding assembly 21 is hindered by the damping oil 22 and moves slowly downward, so that the rolling cylinder 23 drives the dehydration box 40 to roll, and the telescopic arm 31 slides along the top of the shell 10 to adjust the overall length of the limiting mechanism 30 .
[0031] It should be noted that the dehydration box 40 includes a box body and a box cover, and a plurality of through holes are provided on the box body and the box cover for ethanol to enter the dehydration box 40 .
[0032] In this embodiment, the agarose block containing the organoids is placed in the dehydration box 40. After the tumbling mechanism 20 is actuated, the sliding assembly 21 moves downward and drives the rolling drum 23 to rotate. The rolling drum 23 drives the dehydration box 40 to rotate, and the agarose block containing the organoids tumbles, thereby reducing the incomplete contact between the traditional static agarose block and ethanol and improving the dehydration efficiency. During the downward movement of the sliding assembly 21, the damping oil 22 hinders the downward movement of the sliding assembly 21, thereby slowing down the downward movement of the sliding assembly 21, so that the agarose block can keep tumbling during the time-consuming dehydration process. After the telescopic arm 31 slides along the top of the shell 10, the distance between the ends of the telescopic arm 31 increases or decreases, so as to adjust the overall length of the limiting mechanism 30, so as to facilitate mounting on cylinders of different calibers and improve applicability.
[0033] like Figure 1 and Figure 2 As shown, the tumbling mechanism 20 also includes a sealed box 24 fixedly arranged on the top of the shell 10, the sliding component 21 and the damping oil 22 are both arranged inside the sealed box 24, the sliding component 21 is slidably connected to the sealed box 24 in the vertical direction, the upper end of the sliding component 21 is fixedly connected to a counterweight box 25, the lower end of the sliding component 21 is fixedly connected to a rack 26, the rolling cylinder 23 is rotatably connected to the inner walls on both sides of the sealed box 24, the rolling cylinder 23 is fixedly connected to a gear 231, and the gear 231 is meshed with the rack 26.
[0034] It should be noted that the upper end of the sliding component 21 is rotatably connected to the sealing box 24 , and the upper end of the sliding component 21 is also slidably connected to the sealing box 24 along the vertical direction.
[0035] In this embodiment, the counterweight box 25 drives the sliding assembly 21 to slide downward along the inside of the sealing box 24 under the action of its own gravity, and the sliding assembly 21 drives the rack 26 to move downward. The rack 26 drives the rolling drum 23 to rotate along the shell 10 through the gear 231, and the rolling drum 23 drives the dehydration box 40 to rotate, thereby causing the agarose block containing organoids inside the dehydration box 40 to roll. During the downward movement of the sliding assembly 21, the damping oil 22 on the side of the sliding assembly 21 slows down the speed of the sliding assembly 21 moving downward, thereby reducing the rotation speed of the counterweight box 25, which facilitates the agarose block to slowly roll during the time-consuming dehydration process.
[0036] like Figure 2 and Figure 4 As shown, the sliding assembly 21 includes a slider 27 slidably connected to the inside of the sealing box 24, and the inner circumference of the slider 27 is provided with an oil drain hole 271 and a plurality of first oil holes 272, and the aperture sizes of the plurality of first oil holes 272 are different. A stopper 28 is rotatably installed on the top of the slider 27, and the stopper 28 is rotatably connected to the sealing box 24, and a second oil hole 281 is provided inside the stopper 28.
[0037] It should be noted that the aperture of the oil drain hole 271 is larger than the aperture of the first oil hole 272, the aperture of the second oil hole 281 is larger than the aperture of the oil drain hole 271, the upper end of the block 28 is fixedly connected to the counterweight box 25, the lower end of the slider 27 is fixedly connected to the rack 26, the block 28 is also slidably connected to the sealing box 24 along the vertical direction, a cover plate 29 is provided above the block 28, the cover plate 29 is fixedly connected to the slider 27, the inner diameter of the cover plate 29 is smaller than the outer diameter of the slider 27, and is used to prevent the block 28 from being separated from the top of the slider 27, so that the process of the block 28 rotating along the top of the slider 27 is more stable.
[0038] In this embodiment, before dehydration, the counterweight box 25 is rotated, and the counterweight box 25 drives the block 28 to rotate in the slider 27, so that the second oil hole 281 corresponds to the oil drain hole 271, and the counterweight box 25 is moved upward, so that the counterweight box 25 drives the block 28 and the slider 27 to move upward. Since the aperture of the oil drain hole 271 is large, it is convenient for the damping oil 22 above the block 28 to move to the bottom of the slider 27 through the oil drain hole 271. At this time, the block 28 blocks the first oil hole 272, preventing the damping oil 22 above the block 28 from passing through the first oil hole 272. When dehydration is required, the counterweight box 25 is rotated, and the counterweight box 25 drives the block 28 to rotate in the slider 27, so that the second oil hole 281 corresponds to an oil drain hole 271, and the counterweight box 25 Moving downward under the action of its own gravity, the counterweight box 25 drives the block 28 and the slider 27 to move downward. Since the aperture of the first oil hole 272 is small, the speed at which the damping oil 22 below the slider 27 moves to the top of the block 28 through the first oil hole 272 is small, which facilitates the sliding component 21 to move slowly downward in the damping oil 22, so that the agarose block can slowly roll over during the time-consuming dehydration process. In the process of the rotation of the block 28, by adjusting the rotation angle of the second oil hole 281, the second oil hole 281 is made to correspond to the first oil hole 272 of a suitable aperture, so as to adjust the moving speed of the damping oil 22 through the first oil hole 272, and then adjust the downward moving speed of the sliding component 21 to adjust the rolling speed of the agarose block.
[0039] like Figure 1 and Figure 2 As shown, the counterweight box 25 includes a box body 251 fixedly connected to the upper end of the slider 27, and a counterweight hole 252 is opened on the top of the box body 251.
[0040] In this embodiment, counterweight sand is added to the interior of the box body 251 through the counterweight hole 252, so that the overall weight of the counterweight box 25 is changed to adjust the force with which the counterweight box 25 drives the sliding assembly 21 to move downward, so that the speed at which the sliding assembly 21 squeezes the damping oil 22 below to the top is changed, thereby adjusting the speed at which the sliding assembly 21 moves downward to adapt to dehydration processes of different time-consuming times.
[0041] like Figure 1 and Figure 2 As shown, the limiting mechanism 30 further includes a slide groove 32 fixedly connected to the top of the housing 10 , and the telescopic arm 31 is slidably mounted on the inner side of the slide groove 32 .
[0042] It should be noted that the cross-sectional shapes of the telescopic arm 31 and the slide slot 32 are both rectangular.
[0043] In this embodiment, after the telescopic arm 31 slides along the slide groove 32 , the overall length of the limiting mechanism 30 is adjusted, so that the device can be placed on cylinders of different calibers through the limiting mechanism 30 .
[0044] like Figure 3 As shown, a plurality of first limiting holes 311 are formed inside the telescopic arm 31 , a second limiting hole 321 is formed inside the slide slot 32 , and the second limiting hole 321 is slidably connected to the first limiting hole 311 via a limiting pin 33 .
[0045] In this embodiment, when it is necessary to adjust the overall length of the limiting mechanism 30, the limiting pin 33 is pulled out from the inside of the first limiting hole 311 and the second limiting hole 321, and the telescopic arm 31 is slid along the slide groove 32 so that the second limiting hole 321 corresponds to the first limiting hole 311 at an appropriate position, and the limiting pin 33 is slidably inserted into the inside of the first limiting hole 311 and the second limiting hole 321 to form a limit on the telescopic arm 31 and the slide groove 32, so as to facilitate the adjustment of the overall length of the limiting mechanism 30.
[0046] The working principle of the utility model is as follows: the weight box 25 drives the sliding assembly 21 to move slowly downward in the damping oil 22 under the action of its own gravity, the sliding assembly 21 drives the rack 26 to move downward, the rack 26 drives the rolling cylinder 23 to rotate through the gear 231, and the rolling cylinder 23 drives the sealing box 24 to rotate, so that the agarose block containing the organoid inside the sealing box 24 rolls, reducing the incomplete contact between the traditional static agarose block and ethanol, improving the dehydration efficiency, and by sliding the telescopic arm 31 along the shell 10 to increase or decrease the distance between the ends of the telescopic arm 31, the overall length of the limiting mechanism 30 is adjusted, so that it is convenient to be mounted on cylinders of different calibers, thereby improving applicability.
[0047] The above is only a preferred embodiment of the present invention. It should be noted that, for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of this utility model, and these improvements and modifications should also be regarded as the protection scope of this utility model. The structures, devices and operating methods not specifically described and explained in this utility model shall be implemented according to the conventional means in this field unless otherwise specified and limited.
Claims
1. A pretreatment device for organoid embedding, characterized in that: It comprises a housing (10), and also comprises: A rolling mechanism (20), the rolling mechanism (20) being arranged inside the housing (10), the rolling mechanism (20) comprising a sliding assembly (21), damping oil (22) and a rolling cylinder (23); A limiting mechanism (30), wherein two limiting mechanisms (30) are provided and are respectively located at two ends of the top of the shell (10), and the limiting mechanism (30) comprises a telescopic arm (31); A dehydration box (40), wherein two dehydration boxes (40) are provided and are respectively fixedly connected to the two ends of the rolling drum (23); The sliding assembly (21) is hindered by the damping oil (22) and moves slowly downward, so that the rolling cylinder (23) drives the dehydration box (40) to roll, and the telescopic arm (31) slides along the top of the shell (10) to adjust the overall length of the limiting mechanism (30).
2. The pretreatment device for organoid embedding according to claim 1, characterized in that: The tumbling mechanism (20) further comprises a sealing box (24) fixedly arranged on the top of the housing (10); the sliding assembly (21) and the damping oil (22) are both arranged inside the sealing box (24); the sliding assembly (21) and the sealing box (24) are slidably connected in a vertical direction; the upper end of the sliding assembly (21) is fixedly connected to a counterweight box (25); the lower end of the sliding assembly (21) is fixedly connected to a rack (26); the rolling cylinder (23) is rotatably connected to the inner walls on both sides of the sealing box (24); the rolling cylinder (23) is fixedly connected to a gear (231); the gear (231) is meshed with the rack (26).
3. The pretreatment device for organoid embedding according to claim 2, characterized in that: The sliding assembly (21) comprises a sliding block (27) slidably connected to the inside of a sealing box (24); an oil drain hole (271) and a plurality of first oil holes (272) are circumferentially opened inside the sliding block (27); the aperture sizes of the plurality of first oil holes (272) are different; a stopper (28) is rotatably mounted on the top of the sliding block (27); the stopper (28) is rotatably connected to the sealing box (24); and a second oil hole (281) is opened inside the stopper (28).
4. The pretreatment device for organoid embedding according to claim 3, characterized in that: The counterweight box (25) comprises a box body (251) fixedly connected to the upper end of the slider (27), and a counterweight hole (252) is provided on the top of the box body (251).
5. The pretreatment device for organoid embedding according to claim 1, characterized in that: The limiting mechanism (30) further comprises a slide groove (32) fixedly connected to the top of the housing (10), and the telescopic arm (31) is slidably mounted on the inner side of the slide groove (32).
6. The pretreatment device for organoid embedding according to claim 5, characterized in that: A plurality of first limiting holes (311) are provided inside the telescopic arm (31), a second limiting hole (321) is provided inside the sliding groove (32), and the second limiting hole (321) is slidably connected to the first limiting hole (311) by a limiting pin (33).