Constant-temperature fluorescence detector and detection system

Abstract

The utility model provides a constant temperature fluorescence detector and a detection system, and relates to the technical field of medical instruments, the constant temperature fluorescence detector comprises a shell, a multi-channel fluorescence detection module, a uniform mixing module, a preheating module, a first heating module and a controller electrically connected with the multi-channel fluorescence detection module, the uniform mixing module, the preheating module and the first heating module, a mounting groove for mounting a micro-fluidic chip is formed between the multi-channel fluorescence detection module and the first heating module; a preheating module and / or a uniform mixing module for uniformly mixing the micro-fluidic chip are / is arranged in each mounting groove; the preheating module comprises a second heating module for preheating and a driving piece matched with a switch valve positioned at the upper part of the micro-fluidic chip. The pretreatment of uniform mixing and heating on the micro-fluidic chip is realized, and the detection convenience and the detection efficiency are improved.

Description

Technical Field

[0001] The utility model relates to the technical field of medical equipment, and in particular to a constant temperature fluorescence detector and a detection system. Background Art

[0002] Biological reactions such as multiple nucleic acid detection, chemiluminescence multiple indicators or biochemical multiple indicators require multiple detection. For example, Chinese patent CN114047170B discloses a constant temperature fluorescence detector, which includes a shell and a multi-channel fluorescence detection structure of a heating module. The heating module and the multi-channel fluorescence detection structure are arranged in the shell, and an installation groove for installing a microfluidic chip is formed between the heating module and the multi-channel fluorescence detection structure. In practice, before the microfluidic chip is inserted into the detector for detection, the detection item needs to use an additional instrument to mix the detection liquid in the microfluidic chip in advance. In particular, for detection items such as tuberculosis that require heating pre-treatment, the process can only be completed by an additional instrument before the detector is used for detection, which causes great inconvenience and affects the detection efficiency.

[0003] Therefore, the utility model proposes a constant temperature fluorescence detector and a detection system. Utility Model Content

[0004] In view of the deficiencies in the prior art, the utility model proposes a constant temperature fluorescence detector and a detection system, which realizes the pre-treatment of mixing and / or heating the microfluidic chip, thereby improving the convenience and efficiency of detection.

[0005] The technical solution of the utility model is achieved in this way:

[0006] A constant temperature fluorescence detector comprises a shell, a multi-channel fluorescence detection module, a mixing module, a preheating module and a first heating module, and a controller electrically connected to the multi-channel fluorescence detection module, the mixing module, the preheating module and the first heating module; an installation groove for installing a microfluidic chip is formed between the multi-channel fluorescence detection module and the first heating module; a preheating module and / or a mixing module for mixing the microfluidic chip are respectively arranged in the installation groove; the preheating module comprises a second heating module for preheating and a driving member adapted to a switch valve located on the upper part of the microfluidic chip.

[0007] Preferably, a mounting seat is provided in the shell, and the mixing module, preheating module, first heating module and multi-channel fluorescence detection module are all connected to the mounting seat; the second heating module and the driving member are arranged above the first heating module, and the second heating module is arranged on the adjacent side of the driving member.

[0008] Preferably, the mixing module comprises a vibrating member, and the vibrating member is connected to the first heating module via a connecting member.

[0009] Preferably, the second heating module includes a heating film for heating the upper part of the fluidic chip and a fixing member for fixing the heating film, wherein the fixing member is provided with a torsion spring for making the heating film close to the microfluidic chip; the heating film is electrically connected to the controller.

[0010] Preferably, a card insertion detection module is provided in the installation slot.

[0011] Preferably, it further comprises a third heating module, wherein the third heating module is arranged on the other side of the mounting groove opposite to the first heating module; the position of the third heating module is flush with the first heating module.

[0012] Preferably, it further comprises a cover plate, the shell is provided with an insertion hole for inserting the microfluidic chip, and the cover plate is rotatably connected to the shell; a Hall sensor is provided in the shell, and a sensing element adapted to the Hall sensor is provided on the cover plate.

[0013] Preferably, it further comprises a display module for human-computer interaction, and the display module is electrically connected to the controller.

[0014] The utility model also provides a constant temperature fluorescence detection system, including a constant temperature fluorescence detector and a microfluidic chip, wherein the microfluidic chip is provided with a switch valve adapted to a driving member of a preheating module, and the switch valve is used to control the flow of detection liquid into a reaction chamber of the microfluidic chip.

[0015] Preferably, the switch valve is provided with a switch gear for opening and closing, and the output end of the driving member is provided with a transmission gear meshing with the switch gear.

[0016] Compared with the prior art, the utility model has the following advantages:

[0017] By adopting this scheme, for detection items that require heating pretreatment, when the microfluidic chip is inserted into the installation groove, the switch valve of the microfluidic chip is closed, so that the detection liquid stays, and the second heating module is executed to preheat the detection liquid; after preheating, the driving member opens the switch valve, and the detection liquid flows to the reaction chamber. Under the action of the working environment provided by the first heating module, after the mixing module mixes the detection liquid in the reaction chamber of the microfluidic chip, the multi-channel fluorescence detection module starts the detection work. For detection items that do not require preheating, the mixing module is directly used to mix the detection liquid in the reaction chamber of the microfluidic chip before starting the detection work, thereby realizing the pretreatment of mixing and / or heating the microfluidic chip, and improving the detection convenience and efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative labor.

[0019] Figure 1 This is a schematic diagram of the explosion structure of a constant temperature fluorescence detector of the utility model;

[0020] Figure 2 for Figure 1 Schematic diagram of the explosion structure of the middle A part;

[0021] Figure 3 for Figure 1 Schematic diagram of the explosion structure of the middle B part;

[0022] Figure 4 It is a schematic diagram of the explosion structure of the mixing module;

[0023] Figure 5 It is a schematic diagram of the explosion structure of the preheating module;

[0024] Figure 6 This is a schematic cross-sectional structure diagram of a constant temperature fluorescence detector of the utility model;

[0025] Figure 7 It is a schematic diagram of the working principle of the utility model;

[0026] Figure 8 It is a schematic diagram of the working principle of the microfluidic chip and the preheating module;

[0027] Figure identification: 1-shell; 11-cover; 111-Hall sensor; 12-mounting seat; 13-mounting slot; 131-insertion hole; 14-card detection module; 2-multi-channel fluorescence detection module; 3-mixing module; 31-vibrating member; 32-connecting member; 4-second heating module; 41-heating film; 42-fixing member; 43-torsion spring; 5-first heating module; 6-driving member; 61-transmission gear; 7-display module; 8-microfluidic chip; 81-switch valve; 811-switch gear; 9-third heating module. DETAILED DESCRIPTION

[0028] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0029] In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicating the orientation or positional relationship, are based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present invention. In addition, the terms "first", "second", "third", "fourth", etc. are only used for descriptive purposes and cannot be understood as indicating or implying relative importance.

[0030] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "connected" and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0031] Example 1

[0032] This embodiment provides a constant temperature fluorescence detector, such as Figure 1 and Figure 6 As shown, it includes a housing 1, a multi-channel fluorescence detection module 2, a mixing module 3, a preheating module, a first heating module 5 and a controller electrically connected to the multi-channel fluorescence detection module 2, the mixing module 3, the preheating module and the first heating module 5, and a mounting groove 13 for mounting the microfluidic chip 8 is formed between the multi-channel fluorescence detection module 2 and the first heating module 5; the mounting groove 13 is respectively provided with a preheating module and a mixing module 3 for mixing the microfluidic chip 8, and the preheating module and the mixing module 3 can be arranged on the same side or the other side, that is, the preheating module and the mixing module 3 are installed on the same side of the mounting groove 13, or the preheating module is installed on one side of the mounting groove 13, and the mixing module 3 is installed on the other side. The preheating module and the mixing module 3 can be arranged on the same side or the other side, that is, the preheating module and the mixing module 3 are installed on the same side of the mounting groove 13, or the preheating module is installed on one side of the mounting groove 13, and the mixing module 3 is installed on the other side.

[0033] The first heating module 5 is used to provide a working environment for the microfluidic chip 8 to complete detection; in a constant temperature fluorescence detector disclosed in Chinese patent CN114047170B, the first heating module 5 and the multi-channel fluorescence detection module 2 are established prior art, and this device can adopt the above prior art to realize the working principle.

[0034] The preheating module comprises a second heating module 4 for preheating and a driving member 6 adapted to a switch valve 81 located on the upper part of the microfluidic chip 8 .

[0035] In this embodiment, a mounting seat 12 is provided in the shell 1, and the mixing module 3, the preheating module, the first heating module 5 and the multi-channel fluorescence detection module 2 are all connected to the mounting seat 12; the second heating module 4 and the driving member 6 are arranged above the first heating module 5, and the second heating module 4 is arranged on the adjacent side of the driving member 6. Since the microfluidic chip 8 has some features of the prior art disclosed in CN114047170B, that is, the utility model is also provided with a switch valve 81 to make the detection liquid stagnate in the upper cavity of the microfluidic chip 8, so as to cooperate with the preheating module to complete the preheating. After the preheating is completed, the switch valve 81 is opened, and the detection liquid flows into the reaction chamber to carry out the subsequent mixing and detection processes.

[0036] In this embodiment, the mixing module 3 includes a vibrating member 31, and the vibrating member 31 is connected to the first heating module 5 through a connecting member 32. Figure 4 As shown, the vibrating member 31 uses a vibration motor, which is flexibly connected to the mounting base 12 through a connecting member 32, and is rigidly connected to the first heating module 5. The vibrating member 31 is not limited to a vibration motor, and can be other electronic devices. Its actual function is to mix the detection liquid flowing into the reaction chamber while the first heating module 5 continuously provides the required temperature (working environment) for detection.

[0037] In this embodiment, the second heating module 4 includes a heating film 41 for heating the upper part of the microfluidic chip 8 and a fixing member 42 for fixing the heating film 41. The fixing member 42 is provided with a torsion spring 43 for making the heating film 41 close to the microfluidic chip 8. The heating film 41 is electrically connected to the controller; Figure 5 As shown, the fixing member 42 is rigidly connected to the mounting base 12 as a structural member. At the same time, through the setting of the torsion spring 43, when the microfluidic chip 8 is inserted, the torsion spring 43 will apply elastic force to the heating film 41, so that the heating film 41 is tightly attached to the upper part of the microfluidic chip 8. The selection of the heating film 41 is not limited to this, and it can be other electronic components used for heating.

[0038] In this embodiment, a third heating module 9 is further included. The third heating module 9 is disposed on the other side of the mounting groove 13 opposite to the first heating module 5. The position of the third heating module 9 is flush with the first heating module 5. Figure 3 and Figure 6 As shown, in actual operation, the third heating module 9 is adaptively connected to the mounting seat 12, and cooperates with the first heating module to achieve double-sided heating of the reaction chamber to improve the stability of the internal working temperature.

[0039] In this embodiment, a card insertion detection module 14 is provided in the installation slot 13, and the card insertion detection module 14 is electrically connected to the controller, that is, when the microfluidic chip 8 is inserted into the installation slot 13, the controller can feedback the situation to the display module of the human-computer interaction, such as Figure 3 As shown, the card insertion detection module 14 is provided with a rotating member (not marked in the figure) for detection, through which the rotating member is displaced to detect whether the microfluidic chip 8 is inserted.

[0040] In this embodiment, if Figure 6 As shown, it also includes a cover plate 11, and the shell 1 is provided with an insertion hole 131 for inserting the microfluidic chip 8, and the cover plate 11 is rotatably connected to the shell 1; a Hall sensor 111 is provided in the shell 1, and a sensing element adapted to the Hall sensor 111 is provided on the cover plate 11, that is, when the detection work is actually performed, when the cover plate 11 is accidentally opened, the sensing element changes, and the Hall sensor 111 transmits an electrical signal to the controller to stop the detection project. The Hall sensor 111 can be used to determine whether an accident occurs and whether the microfluidic chip 8 being detected needs to be scrapped.

[0041] In this embodiment, a display module 7 for human-computer interaction is also included, and the display module 7 is electrically connected to the controller.

[0042] Working principle: For detection items that require heating pre-treatment, when the microfluidic chip 8 is inserted into the installation groove 13, the switch valve 81 of the microfluidic chip 8 is closed, so that the detection liquid stays until the second heating module 4 performs preheating on the detection liquid; after preheating, the driving member 6 opens the switch valve 81, and the detection liquid flows into the reaction chamber. Under the action of the working environment provided by the first heating module 5, after the mixing module 3 mixes the detection liquid in the reaction chamber of the microfluidic chip 8, the multi-channel fluorescence detection module 2 starts the detection work. For detection items that do not require preheating, the mixing module 3 is directly used to mix the detection liquid in the reaction chamber of the microfluidic chip 8 before starting the detection work, thereby realizing the pre-treatment of mixing and / or heating the microfluidic chip 8, thereby improving the detection convenience and detection efficiency.

[0043] Example 2

[0044] This embodiment provides a constant temperature fluorescence detection system, such as Figure 7 and Figure 8As shown, it includes a constant temperature fluorescence detector and a microfluidic chip 8, and the microfluidic chip 8 is provided with a switch valve 81 adapted to the driving part 6 of the preheating module; the switch valve 81 is used to control the flow of detection liquid into the reaction chamber of the microfluidic chip 8, such as the microfluidic chip 8 disclosed in the prior art CN114047170B. This embodiment adds a switch valve 81 based on the prior art to cooperate with the preheating module.

[0045] In this embodiment, if Figure 8 As shown, a switch gear 811 for opening and closing is provided on the switch valve 81, and a transmission gear 61 meshing with the switch gear 811 is provided at the output end of the driving member 6, that is, in actual operation, the driving member 6 can be driven by a motor or an electric motor, and its output shaft is connected with the switch gear 811, and the switch gear 811 drives the switch valve 81 by meshing the driving transmission gear 61 so that the detection liquid flows into the reaction chamber.

[0046] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention shall be included in the protection scope of the present invention.

Claims

1. A constant temperature fluorescence detector, characterized in that: The invention comprises a housing (1), a multi-channel fluorescence detection module (2), a mixing module (3), a preheating module, a first heating module (5), and a controller electrically connected to the multi-channel fluorescence detection module (2), the mixing module (3), the preheating module, and the first heating module (5); a mounting groove (13) for mounting a microfluidic chip (8) is formed between the multi-channel fluorescence detection module (2) and the first heating module (5); the preheating module and / or the mixing module (3) for mixing the microfluidic chip (8) are respectively arranged in the mounting groove (13); The first heating module (5) is used to provide a working environment for the microfluidic chip (8) to complete detection; The preheating module comprises a second heating module (4) for preheating and a driving member (6) adapted to a switch valve (81) located on the upper part of the microfluidic chip (8).

2. A constant temperature fluorescence detector according to claim 1, characterized in that: A mounting seat (12) is provided in the housing (1), and the mixing module (3), the preheating module, the first heating module (5) and the multi-channel fluorescence detection module (2) are all connected to the mounting seat (12); the second heating module (4) and the driving member (6) are arranged above the first heating module (5), and the second heating module (4) is arranged on the adjacent side of the driving member (6).

3. A constant temperature fluorescence detector according to claim 2, characterized in that: The mixing module (3) comprises a vibrating member (31), and the vibrating member (31) is connected to the first heating module (5) via a connecting member (32).

4. A constant temperature fluorescence detector according to claim 2, characterized in that: The second heating module (4) comprises a heating film (41) for heating the upper part of the microfluidic chip (8) and a fixing member (42) for fixing the heating film (41), wherein a torsion spring (43) is arranged inside the fixing member (42) for making the heating film (41) close to the microfluidic chip (8); the heating film (41) is electrically connected to the controller.

5. A constant temperature fluorescence detector according to claim 2, characterized in that: It also comprises a third heating module (9), which is arranged on the other side of the installation groove (13) opposite to the first heating module (5); the position of the third heating module (9) is flush with the first heating module (5).

6. A constant temperature fluorescence detector according to claim 1, characterized in that: A card insertion detection module (14) is arranged in the installation slot (13).

7. A constant temperature fluorescence detector according to claim 5, characterized in that: The invention also comprises a cover plate (11); the shell (1) is provided with an insertion hole (131) for inserting the microfluidic chip (8); the cover plate (11) is rotatably connected to the shell (1); a Hall sensor (111) is provided in the shell (1); and the cover plate (11) is provided with a sensing element adapted to the Hall sensor (111).

8. A constant temperature fluorescence detector according to claim 1, characterized in that: It also includes a display module (7) for human-computer interaction, and the display module (7) is electrically connected to the controller.

9. A constant temperature fluorescence detection system, comprising a constant temperature fluorescence detector and a microfluidic chip (8) according to any one of claims 1 to 8, wherein the microfluidic chip (8) is provided with a switch valve (81) adapted to a driving component (6) of a preheating module; the switch valve (81) is used to control the flow of detection liquid into a reaction chamber of the microfluidic chip (8).

10. A constant temperature fluorescence detection system according to claim 9, characterized in that: The switch valve (81) is provided with a switch gear (811) for opening and closing, and the output end of the driving member (6) is provided with a transmission gear (61) meshing with the switch gear (811).

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