Sterile subpackaging device for lentiviral vectors

Abstract

The utility model discloses a lentiviral vector sterile subpackaging device which comprises a sterile box, the front side of the sterile box is provided with a cover plate and a box door, the front side of the cover plate is fixedly connected with a PLC, the inner side of the sterile box is fixedly connected with a containing box, the lower side of the containing box is fixedly connected with a discharging pipe, and the lower side of the discharging pipe is fixedly connected with a conveying belt. A feeding pipe is fixedly connected to the right side of the containing box, a subpackaging unit is arranged on the inner side of the sterile box, supporting legs are fixedly connected to the left side and the right side of the sterile box, the subpackaging unit comprises lifting grooves, the lifting grooves are formed in the left inner wall and the right inner wall of the sterile box, and lifting blocks are slidably connected to the inner sides of the lifting grooves in the left side and the right side. By arranging the lifting groove, the lifting block, the connecting plate, the drawing plate, the connecting frame, the rotating shaft, the rotating disc, the screw rod and the split charging pipes, the multiple split charging pipes can be conveniently controlled to carry out lentiviral vector split charging operation in the sterile box, the workload of workers holding the split charging pipes for split charging is reduced, and operation is easy.

Description

Technical Field

[0001] The utility model relates to the field of lentiviral vectors, in particular to a lentiviral vector aseptic subpackaging device. Background Art

[0002] Lentiviral vector is a vector used for gene therapy, which is characterized by its ability to introduce foreign genes into host cells and stably express them in host cells. Compared with general viral vectors, lentiviral vectors have higher transfection efficiency and longer expression duration.

[0003] When the lentiviral vector solution is packaged, the staff needs to hold the test tube to package the solution, which is convenient for transporting the lentiviral vector. When the staff holds the test tube for packaging, the test tube is easily contaminated. When the number of test tubes to be packaged is large, the staff needs to repeatedly align the test tube with the discharge pipe of the container containing the lentiviral vector, which is troublesome to operate and increases the workload of the staff. Utility Model Content

[0004] In order to overcome the shortcomings of the prior art of packaging lentiviral vectors in multiple test tubes and the inconvenience of the workers holding the test tubes, one of the purposes of the utility model is to provide a lentiviral vector aseptic packaging device.

[0005] One of the purposes of the utility model is achieved by the following technical solution: a lentiviral vector aseptic subpackaging device, comprising a sterile box: a cover plate and a box door are arranged on the front side of the sterile box, a PLC controller is fixedly connected to the front side of the cover plate, a containing box is fixedly connected to the inner side of the sterile box, a discharge pipe is fixedly connected to the lower side of the containing box, a feed pipe is fixedly connected to the right side of the containing box, a subpackaging unit is arranged on the inner side of the sterile box, and support legs are fixedly connected to the left and right sides of the sterile box;

[0006] The packaging unit includes a lifting groove, which is opened on the left and right inner walls of the sterile box, and the inner sides of the lifting grooves on the left and right sides are slidably connected with lifting blocks, the inner sides of the lifting grooves are rotatably connected with screws, and a connecting plate is fixedly connected between the lifting blocks on the left and right sides, and a pulling groove is opened on the upper side of the connecting plate, and a pulling block is slidably connected to the inner side of the pulling groove, and a pulling plate is fixedly connected to the upper side of the pulling block, and a fixed frame is fixedly connected to the upper side of the pulling plate, and a rotating shaft is rotatably connected to the upper side of the rotating shaft, and a turntable is fixedly connected to the upper side of the turntable. A plurality of placement barrels are fixedly connected to the upper side of the turntable, and a filling tube is arranged on the inner side of the placement barrel.

[0007] According to the aseptic sub-packaging device for lentiviral vectors, bolts are arranged on the outside of the placement tube, so as to limit the position of the sub-packaging tube.

[0008] According to the aseptic packaging device for lentiviral vectors, the screw rod is threadedly connected to the lifting block, so as to facilitate the control of the lifting block to move up and down.

[0009] According to the aseptic packaging device for lentiviral vectors, the lower side of the aseptic box is fixedly connected with a second motor, and the output end of the second motor is fixedly connected to the upper end of the screw, so as to facilitate the control of the rotation of the screw.

[0010] According to the aseptic subpackaging device for lentiviral vectors, a motor 1 is fixedly connected to the upper side of the pull-out plate, and gears are fixedly connected to the outer side of the output end of the motor 1 and the outer side of the rotating shaft.

[0011] According to the aseptic packaging device for lentiviral vectors, the two gears are meshed and connected.

[0012] According to the aseptic packaging device for lentiviral vectors, a handle is fixedly connected to the front side of the pull-out plate to facilitate pulling the pull-out plate.

[0013] Beneficial effects:

[0014] By providing a lifting slot, a lifting block, a connecting plate, a pull-out plate, a connecting frame, a rotating shaft, a turntable, a screw and a filling tube, it is convenient to control multiple filling tubes to perform lentiviral vector filling operations in a sterile box, reducing the workload of staff members who hold the filling tubes for filling, and the operation is simple.

[0015] Additional aspects and advantages of the present invention will be given in part in the following description, and in part will become apparent from the following description, or will be learned through the practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] The utility model is further described below in conjunction with the accompanying drawings and embodiments;

[0017] Figure 1 This is an overall three-dimensional structural diagram of a lentiviral vector sterile packaging device of the utility model;

[0018] Figure 2 This is a three-dimensional internal structure diagram of a lentiviral vector sterile packaging device of the utility model;

[0019] Figure 3 for Figure 2 The enlarged view of point A in the middle;

[0020] Figure 4 It is a partial three-dimensional structural diagram of a packaging unit in a sterile packaging device for lentiviral vectors of the utility model;

[0021] Figure 5 for Figure 4 Enlarged view of point B in the middle.

[0022] Legend:

[0023] 1. Sterile box; 2. Support legs; 3. Packing unit; 301. Lifting slot; 302. Screw; 303. Lifting block; 304. Connecting plate; 305. Pull-out slot; 306. Pull-out block; 307. Pull-out plate; 308. Handle; 309. Fixed frame; 310. Rotating shaft; 311. Turntable; 312. Placement cylinder; 313. Bolt; 314. Packing tube; 315. Gear; 316. Motor 1; 4. Cover plate; 5. Box door; 6. Motor 2; 7. PLC controller; 8. Container; 9. Discharge pipe; 10. Feed pipe. DETAILED DESCRIPTION

[0024] This section will describe in detail the specific embodiments of the utility model. The preferred embodiments of the utility model are shown in the accompanying drawings. The purpose of the accompanying drawings is to supplement the description of the text part of the specification with graphics, so that people can intuitively and vividly understand each technical feature and the overall technical solution of the utility model, but it cannot be understood as a limitation on the protection scope of the utility model.

[0025] Reference Figure 1-5 A lentiviral vector aseptic packaging device comprises a sterile box 1: a cover plate 4 and a box door 5 are arranged on the front side of the sterile box 1, a PLC controller 7 is fixedly connected to the front side of the cover plate 4, a holding box 8 is fixedly connected to the inner side of the sterile box 1, a discharge pipe 9 is fixedly connected to the lower side of the holding box 8, a feed pipe 10 is fixedly connected to the right side of the holding box 8, a packaging unit 3 is arranged on the inner side of the sterile box 1, and support legs 2 are fixedly connected to the left and right sides of the sterile box 1, wherein a lentiviral vector solution is held in the holding box 8, and the solution is added through the feed pipe 10, a sealing cap is arranged on the outer side of the feed pipe 10 to prevent bacteria from entering the inner side of the sterile box 1, an observation window is arranged on the front side of the cover plate 4 to facilitate observation of the internal packaging situation, and the PLC controller 7 is electrically connected to the motor 1 316 and the motor 2 6 to facilitate the operation of the control device.

[0026] The packaging unit 3 includes a lifting groove 301, which is provided on the left and right inner walls of the sterile box 1. Lifting blocks 303 are slidably connected to the inner sides of the lifting grooves 301 on both sides. Screws 302 are rotatably connected to the inner sides of the lifting grooves 301. A connecting plate 304 is fixedly connected between the lifting blocks 303 on both sides. A drawing groove 305 is provided on the upper side of the connecting plate 304. A drawing block 306 is slidably connected to the inner side of the drawing groove 305. A drawing plate 307 is fixedly connected to the upper side of the drawing block 306. A fixed frame 309 is fixedly connected to the upper side of the drawing plate 307. A rotating shaft 310 is rotatably connected to the upper side of the fixed frame 309. The upper side of the rotating shaft 310 is fixedly connected to the upper side of the rotating shaft 310. A turntable 311 is connected, a plurality of placement cylinders 312 are fixedly connected to the upper side of the turntable 311, a dispensing tube 314 is arranged on the inner side of the placement cylinder 312, the screw 302 is threadedly connected to the lifting block 303, a motor 26 is fixedly connected to the lower side of the sterile box 1, the output end of the motor 26 is fixedly connected to the upper end of the screw 302, a motor 1 316 is fixedly connected to the upper side of the pull-out plate 307, a gear 315 is fixedly connected to the outer side of the output end of the motor 1 316 and the outer side of the rotating shaft 310, the two gears 315 are meshed and connected, a handle 308 is fixedly connected to the front side of the pull-out plate 307, and when dispensing the lentiviral vector solution, the motor 26 is first controlled to start The motor 26 drives the screw 302 to rotate, the screw 302 drives the lifting block 303 to rise, the lifting block 303 drives the connecting plate 304 to rise, the connecting plate 304 drives the dispensing tube 314 on the turntable 311 to be inserted into the discharge pipe 9 at the lower side of the containing box 8, and the outer side of the discharge pipe 9 is provided with a flow pump and a solenoid valve, which is convenient for controlling the quantitative discharge of the lentiviral vector solution in the containing box 8, and dispensing the lentiviral vector solution into the dispensing tube 314, and then controlling the dispensing tube 314 to descend, and then the motor 1 316 is started, and the motor 1 316 drives the gear 315 to rotate. Since the two gears 315 are meshed and connected, the motor 1 316 drives the rotating shaft 310 to rotate, and the rotating shaft 31 0 drives the turntable 311 to rotate, and the turntable 311 drives the filling tube 314 to rotate, so as to facilitate the filling operation on another filling tube 314, and to facilitate the filling of multiple filling tubes 314. After the filling of all the filling tubes 314 is completed, the screw rod 302 is controlled to rotate, and the pull-out plate 307 is controlled to descend to the bottom, and then the box door 5 is opened, and the handle 308 is pulled, and the handle 308 drives the pull-out plate 307 to move, so that the pull-out plate 307 drives the filling tube 314 on the turntable 311 to be pulled out, so as to facilitate the removal of the filling tube 314, reduce the contact of the staff's hands with the filling tube 314 during filling, avoid infection inside the filling tube 314, and reduce the workload of the staff.

[0027] A bolt 313 is provided on the outer side of the placement tube 312 , and by rotating the bolt 313 , the bolt 313 can limit the dispensing tube 314 .

[0028] Working principle: When in use, the staff first places the filling tube 314 on the placing cylinder 312, and then rotates the bolt 313 to limit the filling tube 314, and then pushes the pull-out plate 307, and starts the motor 2 6 to control the screw 302 to rotate. Under the action of the lifting block 303, it is convenient to control the filling tube 314 to rise and dock with the discharge pipe 9, and then perform the filling operation on the filling tube 314, and control the filling tube 314 to descend. The motor 1 316 is started, and under the action of the gear 315 and the rotating shaft 310, it is convenient to control the filling tube 314 on the turntable 311 to rotate, so as to facilitate the filling operation of multiple filling tubes 314. After the filling is completed, the pull-out plate 307 is controlled to descend to the bottom, and the box door 5 is opened, and then the handle 308 is directly pulled to facilitate the pull-out plate 307 to take out the filling tube 314, thereby reducing the workload of the staff and avoiding the filling tube 314 from being infected during the filling.

[0029] The embodiments of the present invention are described in detail above in conjunction with the accompanying drawings, but the present invention is not limited to the above embodiments, and various changes can be made within the knowledge scope of ordinary technicians in the relevant technical field without departing from the purpose of the present invention.

Claims

1. A lentiviral vector aseptic packaging device, characterized in that: The invention comprises a sterile box (1): a cover plate (4) and a box door (5) are arranged on the front side of the sterile box (1); a PLC controller (7) is fixedly connected to the front side of the cover plate (4); a storage box (8) is fixedly connected to the inner side of the sterile box (1); a discharge pipe (9) is fixedly connected to the lower side of the storage box (8); a feed pipe (10) is fixedly connected to the right side of the storage box (8); a subpackaging unit (3) is arranged on the inner side of the sterile box (1); and support legs (2) are fixedly connected to the left and right sides of the sterile box (1); The packaging unit (3) comprises a lifting groove (301), the lifting groove (301) is provided on the left and right inner walls of the sterile box (1), the inner sides of the lifting grooves (301) on the left and right sides are slidably connected with lifting blocks (303), the inner sides of the lifting grooves (301) are rotatably connected with screws (302), a connecting plate (304) is fixedly connected between the lifting blocks (303) on the left and right sides, a drawing groove (305) is provided on the upper side of the connecting plate (304), and the inner side of the drawing groove (305) is provided with a screw rod (302) on the inner side of the lifting groove (301). A pull-out block (306) is slidably connected, a pull-out plate (307) is fixedly connected to the upper side of the pull-out block (306), a fixed frame (309) is fixedly connected to the upper side of the pull-out plate (307), a rotating shaft (310) is rotatably connected to the upper side of the fixed frame (309), a rotating disk (311) is fixedly connected to the upper side of the rotating shaft (310), a plurality of placement tubes (312) are fixedly connected to the upper side of the rotating disk (311), and a dispensing tube (314) is provided inside the placement tube (312).

2. A lentiviral vector aseptic packaging device according to claim 1, characterized in that: Bolts (313) are arranged on the outside of the placement tube (312).

3. The aseptic packaging device for lentiviral vector according to claim 1, characterized in that: The screw rod (302) is threadedly connected to the lifting block (303).

4. The aseptic packaging device for lentiviral vector according to claim 1, characterized in that: The lower side of the sterile box (1) is fixedly connected to a second motor (6), and the output end of the second motor (6) is fixedly connected to the upper end of the screw rod (302).

5. The aseptic packaging device for lentiviral vector according to claim 1, characterized in that: The upper side of the pull-out plate (307) is fixedly connected to a motor 1 (316), and the outer side of the output end of the motor 1 (316) and the outer side of the rotating shaft (310) are both fixedly connected to a gear (315).

6. The aseptic packaging device for lentiviral vectors according to claim 5, characterized in that: The two gears (315) are meshed and connected.

7. The aseptic packaging device for lentiviral vector according to claim 1, characterized in that: A pull handle (308) is fixedly connected to the front side of the pull plate (307).

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