Biological sampling swab capable of storing single-component and double-component reagents

Abstract

The utility model relates to the field of rapid detection of microorganisms, and discloses a biological sampling swab capable of storing single-component and double-component reagents, which comprises a handle, a sleeve, a swab rod, a swab head, a storage tube and a reaction tube, the lower end of the handle is inserted into a top opening of the sleeve, the bottom of the handle is connected with the swab rod, and the lower end of the swab rod is connected with the swab head; the lower-section column body of the storage tube is positioned in the reaction tube and is in close fit with the reaction tube, a sealing ring is arranged between the lower-section column body of the storage tube and the inner surface of the reaction tube, and at least one end surface of the storage tube is sealed by a film; a connecting body of the storage tube and the reaction tube is plugged into the sleeve and is tightly matched with the sleeve; the single-component reagent is stored in the reaction tube or the storage tube, and the double-component reagent is respectively stored in the reaction tube and the storage tube. According to the utility model, the storage of single-component and double-component reagents can be realized, and meanwhile, the storage tube and the reaction tube are directly coupled, so that the problem of liquid leakage in liquid transfer in the prior art is avoided.

Description

Technical Field

[0001] The utility model relates to the field of rapid microbial detection, in particular to a biological sampling swab structure capable of storing single-component and double-component reagents. Background Art

[0002] Biological sampling swabs usually include sampling swabs and cannulas, as well as detection reagents, and are widely used in food hygiene, biochemical research, medical testing, etc. After the sample to be tested is smeared with the sampling swab, the sample and reagent on the swab head react, and the detection signal emitted is measured using a detector, such as an ATP fluorescence detector and a total bacteria meter. Biological sampling swabs play an important role in the effective collection of samples and the accuracy of the final test results. The test items include surface cleanliness detection, protein detection, water quality detection, alkaline phosphatase detection, total bacteria detection, various indicator bacteria, etc. Biological detection reagents often involve different enzymes such as luciferase. Since enzymes are unstable and easily interfered by other chemical substances, their stability and shelf life are reduced. Storing the reaction reagents separately can improve stability. The advantage of single-component reagents is that they are easy to can during production and the swab assembly is simple. Different reagent storage methods are often required for different test items, so single- and double-component storage of detection swabs is helpful for the development and application of biological detection products.

[0003] Patent CN201721613970.4 proposes a two-chamber biological sampling swab, in which the liquid storage cap includes two chambers for storing two liquid components, luciferase and buffer, respectively. A through hole is provided on the partition connecting the two chambers, and a sealing plug is provided on the through hole; the chamber connected to the tube seat is connected by a threaded structure, and the ejector rod passes through the sealing plug when not in use, and there is an opening on the sealing plug. The disadvantage of this scheme is that it can only store liquids and liquid components, and is not suitable for storing single-component reagents. The chamber and the tube seat are fastened by threads. This structure will increase production costs and is prone to leakage. The ejector rod has passed through the sealing plug into the chamber when not in use, which will affect the sealing of the chamber. The liquid storage cap is located at the open end of the reaction tube, and the reagent and sample react at the bottom of the reaction tube. After the reagent is mixed, it enters the reaction tube through the hollow swab rod. The reagent remaining in the liquid storage cap and the swab rod will cause losses. At the same time, since the liquid storage cap is divided into two chambers, the extrusion force will be affected, resulting in the reagent remaining in the chamber.

[0004] Patent CN202121352213.2 proposes a push-type biological sampling swab. The handle of the swab is sealed by several circular washers and an outer casing. The liquid storage tube is located between the barrier layer and the reaction tube, the aluminum foil at the top of the reaction tube and the bottom of the liquid storage tube are in close contact, and the reaction tube and the casing are fastened by threads. If this utility model is used for two-component reagent storage, there are the following shortcomings. The reagent storage tube is sandwiched between the barrier layer in the casing and the reaction tube fastened by threads. The reaction tube and the storage tube are simply superimposed without coupling, which reduces the coaxiality of the reaction tube and the storage tube, affects the effect of the swab rod penetrating the aluminum foil and the amount of reagent flowing into the reaction tube. If the reaction tube is sealed, the reagent can be stored, but if the reaction tube is sealed with aluminum foil, it will cause too many aluminum foil diaphragms, making it difficult to puncture the cotton swab or twisting the cotton swab. In addition, the threaded mouth is sealed with aluminum foil, and the thermal deformation of the tube mouth will affect the assembly of the reaction tube and the casing. Utility Model Content

[0005] The technical problem to be solved by the utility model is that the biological sampling detection reagents in the prior art contain unstable enzyme substances such as luciferase, etc., and storing all the components together will reduce the stability and validity period of the reagents, while storing them in groups can improve the stability of the reagents. The prior art lacks biological sampling swabs that can store liquid / liquid components, or liquid / solid components at the same time. At the same time, the snap-off swabs are commonly used at present. The reaction tube is long and cannot be effectively sealed, making it difficult to use for storing reagents. The storage tube away from the reaction tube is designed into two chambers to store two components. The poor independence of the two chambers will affect the airtightness, and the threaded tight-fitting structure is also prone to leakage.

[0006] In order to solve the above technical problems, the technical solution of the utility model is a biological sampling swab capable of storing single-component and double-component reagents, comprising a sampling handle, a sleeve, a swab rod, a swab head, a storage tube and a reaction tube.

[0007] The handle is provided with a socket, one end of the swab rod is inserted into the socket and fits tightly, and the other end is connected to the swab head. The lower end of the handle is inserted into the top opening of the sleeve. The storage tube includes an upper column and a lower column, and a sealing gasket is provided on the cylindrical surface or the bottom end surface of the lower column, and the lower column is located in the reaction tube and fits tightly therewith. The upper column of the storage tube and the sleeve are tightly fitted, and at least one end surface of the storage tube is sealed with a sealing film. The connector of the storage tube and the reaction tube is inserted into the sleeve from one side of the storage tube, and the inserted part is tightly fitted with the sleeve. When storing a single-component reagent in a reaction tube, any end of the storage tube can be sealed, and the sealing bottom end can maintain the independence of the two storage tubes, while the sealing top end can increase the volume of the stored reagent; when storing a single-component reagent in a storage tube or storing a two-component reagent, both ends are sealed.

[0008] By adopting the above technical solution, the reaction tube can be used to store reagents. After storing the reagents, the storage tube is sealed with the first film and the second film, and will not interfere with the reagents in the reaction tube. The reaction tube can be sealed by installing a sealing ring storage tube, and a single-component reagent can be stored at the same time. Compared with the design in which the ejector rod is inserted into the cavity and a through hole is provided on the sealing plug in the above-mentioned two-cavity combined detection swab, the storage tube and the reaction tube are always kept airtight before use, and no other structures, especially through holes, are provided on the sealing material. At the same time, the conventional method uses the sampling handle as the reagent storage area, and the handle cannot be moved. The reagent needs to be transferred to the bottom of the reaction tube by squeezing the reagent storage area. The utility model enables the swab head for collecting samples to move relative to the reagent without moving the reagent over a long distance, thereby avoiding the loss of the reagent during the transfer process and the leakage problem between accessories.

[0009] Preferably, the handle is provided with a plurality of fastening plates, and the fit force between the fastening plates and the sleeve is moderate, and the handle and the sleeve are controlled to be coaxial when stationary or in relative motion. This structure can provide a moderate tight fit force, and the handle can be moved by applying a certain external force, and the handle is fixed relative to the sleeve without applying force, thereby avoiding the problem of too loose or too tight when the handle and the sleeve move relative to each other.

[0010] Preferably, the sleeve is a thin-walled plastic tube with a wall thickness that is not too thick so that the tube wall maintains a certain elasticity.

[0011] Preferably, the reaction tube is provided with a first raised ring or the storage tube is provided with a second raised ring, and the bottom end of the sleeve is in contact with the first raised ring or the second raised ring; the first raised ring or the second raised ring plays a limiting role.

[0012] Preferably, the swab head is made of rayon, polyester fiber, or flocking fiber, which can effectively collect samples from the surface of an object.

[0013] In summary, the utility model has at least one of the following beneficial effects:

[0014] 1. The two-component reagents are stored in the reaction tube and the storage tube respectively, and liquid / liquid or liquid / solid components can be stored at the same time, and can also be used for single-component reagent storage; the utility model can be used for the diversified development of detection reagents, improve the stability and durability of reagents, and avoid opening multiple sets of molds, thereby reducing production costs.

[0015] 2. The reaction tube is the reaction area for reagents and samples. The reaction tube and the storage tube coupled with it are directly used to store reagents. The reagents do not need to be moved over long distances. You only need to push the handle to move the sample on the swab head to the bottom of the reaction tube. This can avoid the loss of reagents during transportation and leakage between many accessories.

[0016] 3. The lower column of the storage tube is inserted into the reaction tube, and the seal is achieved through the sealing ring between the lower column and the inner surface of the reaction tube. The connector of the storage tube and the reaction tube is inserted into the sleeve from one side of the storage tube, and the inserted part is tightly matched with the sleeve. The storage tube and the reaction tube are directly coupled, which is more secure and coaxial than the stacking method of the two. In addition, only two sealing films are required at most, and the swab rod is easy to puncture. The sleeve design is simpler, and there is no need to design too many connections and matching structures. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 This is a schematic diagram of the overall structure of the biological sampling swab of the utility model;

[0018] Figure 2 Two installation schematic diagrams of the sealing ring of the utility model;

[0019] Figure 3 A schematic diagram of a storage tube provided with a second raised ring according to the utility model;

[0020] In the figure: 1, handle; 2, fastening plate; 3, socket; 4, sleeve; 5, swab rod; 6, swab head; 7, first film; 8, storage tube; 9, first raised ring; 10, second film; 11, sealing ring; 12, reaction tube; 13, slot; 14, second raised ring; 15, step; 16, top opening DETAILED DESCRIPTION

[0021] The utility model is further described in detail below in conjunction with the accompanying drawings.

[0022] Example 1

[0023] The utility model discloses a biological sampling swab capable of storing single-component and double-component reagents, referring to Figure 1 , including a sampling handle 1, a sleeve 4, a swab rod 5, a swab head 6, a storage tube 8, and a reaction tube 12.

[0024] The handle 1 is provided with a socket 3, one end of the swab rod 5 is inserted into the socket 3 and tightly fits, and the other end is connected to the swab head 6. The lower end of the handle 1 is inserted into the top opening 16 of the sleeve 4; the lower column of the storage tube 8 is located in the reaction tube 12 and tightly fits therewith. The storage tube 8 and the reaction tube 12 are sealed by a sealing ring 11, and the sealing ring 11 is located on the cylindrical surface or the bottom end surface of the lower column of the storage tube 8; when the sealing ring 11 is located at the bottom end of the lower column of the storage tube 8, a step 15 is required to be provided inside the opening of the reaction tube 12, and the sealing gasket is placed between the bottom end of the storage tube 8 and the step 15; the storage tube 8 has at least one end face sealed. The connector of the storage tube 8 and the reaction tube 12 is inserted into the sleeve 4 from the top side of the storage tube 8, and the inserted part is tightly fitted with the sleeve 4, wherein at least one section of the upper column of the storage tube 8 and the tube mouth of the reaction tube 12 is tightly fitted with the sleeve 4, and can be reinforced by adhesive or plastic welding. When storing a single-component reagent in the reaction tube 12, any end of the storage tube 8 can be sealed. The sealing of the bottom end can maintain the independence of the two storage tubes, while the sealing of the top end can increase the volume of the stored reagent. When storing a single-component reagent in the storage tube 8 or storing a two-component reagent, both ends are sealed. When the handle 1 is fully inserted during use, the swab rod 5 will pierce the aluminum foil film, and the swab head 6 will move down to the bottom of the reaction tube. The reagent in the storage tube 8 will fall into the reaction tube 12 under the action of gravity and external force, and the reagent will react with the sample on the swab head 6 after mixing.

[0025] When the two-component reagent in this example is composed of enzyme solution and buffer solution, the buffer solution is stored in the storage tube 8, and the enzyme solution is stored in the reaction tube 12, or vice versa. When the two-component reagent is composed of enzyme lyophilized powder and buffer solution, the buffer solution is stored in the reaction tube 12, and the enzyme lyophilized powder is stored in the storage tube 8, or vice versa. First, install the sealing ring 11 in the card slot 13 of the storage tube 8, then seal the bottom opening of the storage tube 8 with the second film 10, then add the reagent to the storage tube 8, and then seal the top of the storage tube 8 with the first film 7; after adding the reagent to the reaction tube 12, insert the bottom of the storage tube 8 sealed at both ends into the reaction tube 12 until the mouth of the reaction tube 12 contacts the bottom of the upper column of the storage tube 8, and finally insert the top of the storage tube 8 into the sleeve 4. When storing a single-component mixture of enzyme solution and buffer solution, the reagent can be stored in any storage tube.

[0026] The first film and the second film are plastic films or aluminum-plastic composite films. The reaction tube 12 is made of highly transparent plastic material and is used for reagent and sample reactions. The sleeve 4 is a thin-walled plastic tube, and the wall thickness is not easy to be large so that the tube wall maintains a certain elasticity. The swab head 6 can be a fiber-wrapped structure, and its fiber can be polyester fiber, rayon or flocking fiber, which is usually used for surface sampling of objects, or a plastic head containing multiple holes for water sample collection. A slot 13 can be set on the lower cylinder surface of the storage tube 8, but when the lower cylinder is shorter, the slot 13 can be omitted, and the sealing ring 11 can be clamped by the bottom surface of the upper cylinder of the storage tube 8.

[0027] Example 2

[0028] Based on the further improvement of the embodiment 1, the handle 1 is provided with a plurality of fastening pieces 2. The fastening pieces 2 are in the form of sheets, and the matching force between the fastening pieces 2 and the sleeve 4 is moderate, and the handle 1 and the sleeve 4 are controlled to be coaxial when stationary or in relative motion.

[0029] Example 3

[0030] Further improved on the basis of Example 1, the reaction tube 12 is provided with a first raised ring 9 or the storage tube 8 is provided with a second raised ring 14, and the bottom end of the sleeve 4 is in contact with the first raised ring 9 or the second raised ring 14; the first raised ring 9 or the second raised ring 14 plays a limiting role.

[0031] The above is only a preferred embodiment of the utility model, and is not any formal or substantial limitation of the utility model. It should be pointed out that ordinary technicians in this technical field can make some improvements and supplements without departing from the utility model, and these improvements and supplements should also be regarded as the protection scope of the utility model. Any technician familiar with this profession can make some changes, modifications and equivalent changes made by using the technical content disclosed above without departing from the spirit and scope of the utility model, which are all equivalent embodiments of the utility model; at the same time, any changes, modifications and evolutions of any equivalent changes made to the above embodiments based on the essential technology of the utility model are still within the scope of the technical solution of the utility model.

Claims

1. A biological sampling swab capable of storing single-component and double-component reagents, characterized in that: The invention comprises a handle (1), a sleeve (4), a swab rod (5), a swab head (6), a storage tube (8), and a reaction tube (12); the lower end of the handle (1) is inserted into the top opening (16) of the sleeve (4), the bottom of the handle (1) is connected to the swab rod (5), and the lower end of the swab rod (5) is connected to the swab head (6); the lower column of the storage tube (8) is located in the reaction tube (12) and is tightly matched therewith, and a sealing ring (12) is provided between the lower column of the storage tube (8) and the inner surface of the reaction tube (12). 1) Sealing; the storage tube (8) has at least one end face sealed, the top end of the storage tube (8) is sealed with a first film (7), and the bottom end of the storage tube (8) is sealed with a second film (10); the connector between the storage tube (8) and the reaction tube (12) is inserted into the sleeve (4) from the top end side of the storage tube (8), and the inserted part is tightly matched with the sleeve (4); the single-component reagent is stored in the reaction tube (12) or the storage tube (8), and the two-component reagent is stored in the reaction tube (12) and the storage tube (8) respectively.

2. The biological sampling swab capable of storing single-component and double-component reagents according to claim 1, characterized in that: The reaction tube (12) is provided with a first raised ring (9) or the storage tube (8) is provided with a second raised ring (14), and the bottom end of the sleeve (4) is in contact with the first raised ring (9) or the second raised ring (14).

3. The biological sampling swab capable of storing single-component and double-component reagents according to claim 1, characterized in that: The handle (1) is provided with a plurality of fastening plates (2), and the matching force between the fastening plates (2) and the sleeve (4) is moderate.

4. The biological sampling swab capable of storing single-component and double-component reagents according to claim 1, characterized in that The bottom of the handle (1) is provided with an insertion hole (3), and the swab rod (5) is located in the insertion hole (3) and is tightly fitted.

5. The biological sampling swab capable of storing single-component and double-component reagents according to claim 1, characterized in that: The reaction tube (12) is made of high-transparency plastic.

6. The biological sampling swab capable of storing single-component and double-component reagents according to claim 1, characterized in that: The sleeve (4) is a thin-walled plastic tube.

7. The biological sampling swab capable of storing single-component and double-component reagents according to claim 1, characterized in that: The swab head (6) is made of rayon, polyester fiber or flocking material.

8. The biological sampling swab capable of storing single-component and double-component reagents according to claim 1, characterized in that: The first film (7) and the second film (10) are plastic films or aluminum-plastic composite films.

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