Temperature regulation and control device for closed lentiviral vector culture

Abstract

The utility model relates to the technical field of temperature control devices, in particular to a temperature control device for closed lentiviral vector culture, which is characterized in that a mounting base is arranged to drive a temperature sensor to move downwards, and when an upper cover moves to a proper position, a threaded rod is attached to the inner wall of a fixing sleeve, so that the temperature can be controlled only by rotating a shifting plate; a stirring plate is arranged on the culture dish, the stirring plate drives a threaded rod to be in threaded connection to the inner wall of a fixing sleeve to be fixed, and meanwhile one end of each temperature sensor penetrates through a communicating hole and moves to an inner cavity of the culture dish to measure the temperature, and multiple sets of temperature sensors are arranged, generate electric signals according to temperature data and transmit the electric signals to a controller through a wire; the device can simultaneously control the temperature of a plurality of groups of culture dishes so as to reach a proper temperature interval, and when a large batch of lentiviral vectors are cultured, the display screen displays the temperature of the culture dishes in real time, so that time and labor are saved, and the operation time is greatly shortened.

Description

Technical Field

[0001] The utility model relates to the technical field of temperature control devices, in particular to a temperature control device for closed-type lentiviral vector culture. Background Art

[0002] In biomedical research, lentiviral vectors, as an important gene delivery tool, are widely used in gene therapy, gene editing, and drug screening. However, in the process of large-scale production of lentiviral vectors, how to efficiently and accurately culture and sample and analyze them has become an urgent problem to be solved. Especially in large-scale culture experiments, if the experimenters need to sample and analyze the lentivirus in different culture dishes one by one, it will not only greatly increase the operation time, but also the process is cumbersome and has poor practicality.

[0003] In this solution, a temperature control device for closed lentiviral vector culture is therefore needed to improve the above problems. Utility Model Content

[0004] The purpose of the utility model is to provide a temperature control device for closed lentiviral vector culture to solve the problems raised in the above background technology.

[0005] In order to achieve the above purpose, the utility model provides the following technical solutions:

[0006] A temperature control device for closed lentiviral vector culture, comprising a box body, a lifting damper is rotatably connected to the inner wall of the box body, one end of the lifting damper is rotatably connected to a temperature measuring component, an inner pad shell is installed on the inner wall of the box body, a temperature control plate is installed on the inner wall of the inner pad shell, and a culture dish is arranged on the base surface of the temperature control plate;

[0007] The temperature measuring component includes a cover plate, one end of the cover plate is installed at the port of the box body through a hinge, one end of the lifting damper is connected to the inner wall of the cover plate, a handle is installed on the outer wall of the cover plate, a controller is installed on the outer wall of the cover plate, a display screen is installed on the outer wall of the cover plate, a fixing sleeve is installed on the outer wall of the cover plate, a connecting hole is opened on the outer wall of the cover plate, a limiting sleeve is installed on the outer wall of the cover plate, a limiting spring is installed at the port of the limiting sleeve, a sliding rod is slidably connected to the inner wall of the limiting sleeve, an upper cover is installed at one end of the sliding rod, a handle is installed on the outer wall of the upper cover, a threaded rod is slidably connected to the outer wall of the upper cover, one end of the threaded rod is threadedly connected to the inner wall of the fixing sleeve, and a dial plate is installed on the other end of the threaded rod, a mounting base is installed on the inner wall of the upper cover, and a temperature sensor is installed on the base surface of the mounting base.

[0008] As a preferred solution of the utility model, the controller is respectively connected to the temperature control board, the display screen and the temperature sensor through wires and the connection method is electrical connection. The temperature control board is provided in multiple groups and is respectively located on the inner wall of the inner pad shell.

[0009] As a preferred solution of the utility model, the culture dishes are provided in multiple groups and are respectively located in the inner cavity of the inner pad shell, and the culture dishes are located directly below the connecting holes, and the handle is located on one side of the upper cover.

[0010] As a preferred solution of the utility model, the communicating holes are provided in multiple groups and are respectively located on the outer wall of the cover plate, and the limiting sleeves are provided in multiple groups and are respectively located on the outer wall of the cover plate.

[0011] As a preferred solution of the utility model, the limit spring is located on one side of the slide rod, the slide rod is provided with multiple groups and are respectively located on the outer wall of the upper cover, the threaded rod is located on one side of the slide rod, and the mounting base is provided with multiple groups and are respectively located on the outer wall of the upper cover.

[0012] As a preferred solution of the utility model, the temperature sensors are provided in multiple groups and are respectively located on the outer wall of the mounting base, and the temperature sensors are located directly above the culture dish.

[0013] Compared with the prior art, the beneficial effects of the utility model are:

[0014] 1. In the utility model, through the temperature measuring component in the temperature control device for closed lentiviral vector culture, the upper cover drives the mounting base to move downward, so that the mounting base drives the temperature sensor to move downward, and when the upper cover moves to a suitable position, the threaded rod fits on the inner wall of the fixed sleeve, and only the dial plate needs to be rotated to make the dial plate drive the threaded rod to be threadedly connected to the inner wall of the fixed sleeve for fixing, and at the same time, one end of the temperature sensor passes through the connecting hole and moves to the inner cavity of the culture dish for temperature measurement. By setting multiple groups of temperature sensors, and the temperature sensors generate electrical signals according to temperature data and transmit them to the controller through wires, so that the controller controls the temperature control plate to raise and lower the temperature, the device can simultaneously control the temperature of multiple groups of culture dishes to reach a suitable temperature range. When culturing a large number of lentiviral vectors, the display screen simultaneously displays the temperature of the culture dish in real time, which saves time and effort, greatly reduces the operation time, and has better practicality, thereby helping to solve the problem of how to efficiently and accurately culture and sample and analyze lentiviral vectors in the process of large-scale production. Especially when conducting large-scale culture experiments, if the experimenters need to sample and analyze the lentiviruses in different culture dishes one by one, it will not only greatly increase the operation time, but also the process is cumbersome and has poor practicality. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 It is a schematic diagram of the overall structure of the utility model;

[0016] Figure 2 This is a side view of the structure of the utility model;

[0017] Figure 3 This is a schematic diagram of the structure of the temperature measuring component of the utility model.

[0018] In the figure: 1. box body; 2. lifting damper; 3. temperature measuring component; 301. cover plate; 302. handle; 303. controller; 304. display screen; 305. fixing sleeve; 306. connecting hole; 307. limiting sleeve; 308. limiting spring; 309. sliding rod; 310. upper cover; 311. handle; 312. threaded rod; 313. dial plate; 314. mounting base; 315. temperature sensor; 4. inner pad shell; 5. temperature control board; 6. culture dish. DETAILED DESCRIPTION

[0019] The technical solutions in the embodiments of the utility model will be clearly and completely described below in conjunction with the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, rather than all of the embodiments. Based on the embodiments of the utility model, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the utility model.

[0020] For examples, see Figure 1-3 , the utility model provides a technical solution:

[0021] A temperature control device for closed lentiviral vector culture, comprising a box body 1, a lifting damper 2 is rotatably connected to the inner wall of the box body 1, one end of the lifting damper 2 is rotatably connected to a temperature measuring component 3, an inner pad shell 4 is installed on the inner wall of the box body 1, a temperature control plate 5 is installed on the inner wall of the inner pad shell 4, and a culture dish 6 is arranged on the base surface of the temperature control plate 5;

[0022] In this embodiment, please refer to Figure 1 , Figure 2 and Figure 3The temperature measuring component 3 includes a cover plate 301, one end of the cover plate 301 is hingedly mounted at the port of the box body 1, one end of the lifting damper 2 is connected to the inner wall of the cover plate 301, a handle 302 is mounted on the outer wall of the cover plate 301, and the handle 302 is located on one side of the upper cover 310, a controller 303 is mounted on the outer wall of the cover plate 301, a display screen 304 is mounted on the outer wall of the cover plate 301, a fixing sleeve 305 is mounted on the outer wall of the cover plate 301, a connecting hole 306 is opened on the outer wall of the cover plate 301, and the connecting holes 306 are provided in multiple groups and are respectively located on the outer wall of the cover plate 301, a limiting sleeve 307 is installed on the outer wall of the cover plate 301, and the limiting sleeve 307 is provided in multiple groups and is respectively located on the outer wall of the cover plate 301, and a limiting spring 308 is installed at the port of the limiting sleeve 307, and the limiting spring 308 is located on one side of the slide bar 309, and the limiting A sliding rod 309 is slidably connected to the inner wall of the positioning sleeve 307. The sliding rod 309 is provided with multiple groups and are respectively located on the outer wall of the upper cover 310. The upper cover 310 is installed at one end of the sliding rod 309. A handle 311 is installed on the outer wall of the upper cover 310. A threaded rod 312 is slidably connected to the outer wall of the upper cover 310. The threaded rod 312 is located on one side of the sliding rod 309. One end of the threaded rod 312 is threadedly connected to the inner wall of the fixing sleeve 305, and a dial plate 313 is installed on the other end of the threaded rod 312. A mounting base 314 is installed on the inner wall of the upper cover 310. The mounting base 314 is provided with multiple groups and are respectively located on the outer wall of the upper cover 310. A temperature sensor 315 is installed on the base surface of the mounting base 314. The temperature sensor 315 is provided with multiple groups and are respectively located on the outer wall of the mounting base 314, and the temperature sensor 315 is located directly above the culture dish 6.

[0023] Furthermore, the controller 303 is respectively connected to the temperature control board 5, the display screen 304 and the temperature sensor 315 through wires and the connection method is electrical connection, so that the device is powered on, a plurality of temperature control boards 5 are provided and are respectively located on the inner wall of the inner pad shell 4, a plurality of culture dishes 6 are provided and are respectively located in the inner cavity of the inner pad shell 4, and the culture dishes 6 are located directly below the connecting hole 306 for easy detection, and the lifting damper 2 plays a buffering role when the cover plate 301 is opened and closed, preventing the temperature measuring component 3 from hitting the culture dish 6.

[0024] The working process of the utility model is as follows: when the temperature control device for closed lentiviral vector culture designed by the present invention is in operation, the controller 303 is respectively connected to the temperature control board 5, the display screen 304 and the temperature sensor 315 through wires, and the connection mode is electrical connection, so that the device is powered on, and the switch of the controller 303 is turned on, so that the controller 303 controls the temperature control board 5 to operate and heat up, and reaches the set temperature value range, and then the closed lentiviral vector culture is carried out, and the virus vector is placed in the inner cavity of the culture dish 6, and then only the handle 302 needs to be pulled, so that the handle 302 applies a pulling force to the cover plate 301, so that the cover plate 301 When the hinge rotates and the cover 301 is closed, a limiting sleeve 307 is then installed on the outer wall of the cover 301, a limiting spring 308 is installed at the end of the limiting sleeve 307, a sliding rod 309 is slidably connected to the inner wall of the limiting sleeve 307, an upper cover 310 is installed at one end of the sliding rod 309, a handle 311 is installed on the outer wall of the upper cover 310, a threaded rod 312 is slidably connected to the outer wall of the upper cover 310, one end of the threaded rod 312 is threadedly connected to the inner wall of the fixing sleeve 305, and a dial plate 313 is installed at the other end of the threaded rod 312, a mounting base 314 is installed on the inner wall of the upper cover 310, and a base surface of the mounting base 314 is installed With the temperature sensor 315, it is only necessary to press down the upper cover 310 to move the upper cover 310 downward, and the upper cover 310 applies pressure to the limit spring 308 to compress it. At the same time, the upper cover 310 drives the mounting base 314 to move downward, so that the mounting base 314 drives the temperature sensor 315 to move downward. At the same time, when the upper cover 310 moves to a suitable position, the threaded rod 312 fits on the inner wall of the fixing sleeve 305. It is only necessary to rotate the dial plate 313 so that the dial plate 313 drives the threaded rod 312 to be threadedly connected to the inner wall of the fixing sleeve 305 for fixing. At the same time, one end of the temperature sensor 315 passes through the connecting hole 306 and moves to the inner cavity of the culture dish 6. Temperature measurement is performed by setting multiple groups of temperature sensors 315, and the temperature sensors 315 generate electrical signals according to the temperature data and transmit them to the controller 303 through the wire, so that the controller 303 controls the temperature control plate 5 to raise and lower the temperature. The device can simultaneously control the temperature of multiple groups of culture dishes 6 to achieve a suitable temperature range. When culturing a large number of lentiviral vectors, the display screen 304 simultaneously displays the temperature of the culture dishes 6 in real time, which saves time and effort, greatly reduces the operation time, and has better practicality, thereby helping to solve the problem of how to efficiently and accurately cultivate and sample and analyze lentiviral vectors in the process of large-scale production, which has become an urgent problem to be solved. Especially when conducting large-scale culture experiments, if the experimenters need to sample and analyze the lentiviruses in different culture dishes one by one, it not only greatly increases the operation time, but also the process is cumbersome and has poor practicality.

[0025] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A temperature control device for closed lentiviral vector culture, comprising a box body (1), characterized in that: A lifting damper (2) is rotatably connected to the inner wall of the box body (1), one end of the lifting damper (2) is rotatably connected to a temperature measuring component (3), an inner pad shell (4) is mounted on the inner wall of the box body (1), a temperature control plate (5) is mounted on the inner wall of the inner pad shell (4), and a culture dish (6) is arranged on the base surface of the temperature control plate (5); The temperature measuring assembly (3) comprises a cover plate (301), one end of the cover plate (301) is hingedly mounted at a port of the box body (1), one end of the lifting damper (2) is connected to the inner wall of the cover plate (301), a handle (302) is mounted on the outer wall of the cover plate (301), a controller (303) is mounted on the outer wall of the cover plate (301), a display screen (304) is mounted on the outer wall of the cover plate (301), a fixing sleeve (305) is mounted on the outer wall of the cover plate (301), a connecting hole (306) is provided on the outer wall of the cover plate (301), and a limiting sleeve (307) is mounted on the outer wall of the cover plate (301), the limiting sleeve ( A limit spring (308) is installed at the port of the fixing sleeve (307), a sliding rod (309) is slidably connected to the inner wall of the limit sleeve (307), an upper cover (310) is installed at one end of the sliding rod (309), a handle (311) is installed on the outer wall of the upper cover (310), a threaded rod (312) is slidably connected to the outer wall of the upper cover (310), one end of the threaded rod (312) is threadedly connected to the inner wall of the fixing sleeve (305), and a dial plate (313) is installed at the other end of the threaded rod (312), a mounting base (314) is installed on the inner wall of the upper cover (310), and a temperature sensor (315) is installed on the base surface of the mounting base (314).

2. A temperature control device for closed lentiviral vector culture according to claim 1, characterized in that: The controller (303) is respectively connected to a temperature control panel (5), a display screen (304) and a temperature sensor (315) via wires and the connection method is electrical connection. A plurality of groups of temperature control panels (5) are provided and are respectively located on the inner wall of the inner pad shell (4).

3. A temperature control device for closed lentiviral vector culture according to claim 1, characterized in that: The culture dishes (6) are provided in multiple groups and are respectively located in the inner cavity of the inner pad shell (4), and the culture dishes (6) are located directly below the connecting holes (306). The handle (302) is located on one side of the upper cover (310).

4. A temperature control device for closed lentiviral vector culture according to claim 1, characterized in that: The communicating holes (306) are provided in multiple groups and are respectively located on the outer wall of the cover plate (301); the limiting sleeves (307) are provided in multiple groups and are respectively located on the outer wall of the cover plate (301).

5. A temperature control device for closed lentiviral vector culture according to claim 1, characterized in that: The limit spring (308) is located on one side of the slide rod (309), the slide rod (309) is provided in multiple groups and are respectively located on the outer wall of the upper cover (310), the threaded rod (312) is located on one side of the slide rod (309), and the mounting base (314) is provided in multiple groups and are respectively located on the outer wall of the upper cover (310).

6. A temperature control device for closed lentiviral vector culture according to claim 1, characterized in that: The temperature sensors (315) are arranged in multiple groups and are respectively located on the outer wall of the mounting base (314), and the temperature sensors (315) are located directly above the culture dish (6).

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